AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006980


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006980.t1 MTR_7g108250 100.000 102 0 0 1 102 1 102 1.04e-72 211
MS.gene006980.t1 MTR_1g088905 76.471 102 24 0 1 102 1 102 6.34e-57 171
MS.gene006980.t1 MTR_2g019900 52.941 102 48 0 1 102 1 102 8.42e-40 127
MS.gene006980.t1 MTR_1g088895 57.843 102 43 0 1 102 1 102 1.60e-39 127
MS.gene006980.t1 MTR_4g119050 55.340 103 45 1 1 102 1 103 1.89e-39 127
MS.gene006980.t1 MTR_4g119030 55.340 103 45 1 1 102 1 103 1.89e-39 127
MS.gene006980.t1 MTR_4g119040 55.340 103 45 1 1 102 1 103 1.89e-39 127
MS.gene006980.t1 MTR_7g108260 56.436 101 44 0 1 101 1 101 1.10e-36 120
MS.gene006980.t1 MTR_2g019950 46.078 102 55 0 1 102 1 102 2.73e-32 108
MS.gene006980.t1 MTR_1g088910 49.020 102 52 0 1 102 1 102 2.62e-30 103
MS.gene006980.t1 MTR_7g108220 49.515 103 51 1 1 102 1 103 1.64e-29 102
MS.gene006980.t1 MTR_7g108210 48.544 103 52 1 1 102 1 103 9.32e-29 100
MS.gene006980.t1 MTR_7g108200 45.263 95 52 0 1 95 1 95 3.82e-26 93.6
MS.gene006980.t1 MTR_1g088920 43.158 95 54 0 1 95 1 95 1.24e-25 92.0
MS.gene006980.t1 MTR_1g088925 41.053 95 56 0 1 95 1 95 1.83e-25 91.7
MS.gene006980.t1 MTR_7g022690 37.864 103 63 1 1 102 30 132 1.59e-23 87.8
MS.gene006980.t1 MTR_7g022550 37.864 103 63 1 1 102 30 132 2.15e-23 87.4
MS.gene006980.t1 MTR_7g022710 35.922 103 65 1 1 102 30 132 4.13e-23 86.7
MS.gene006980.t1 MTR_7g026770 39.806 103 61 1 1 102 22 124 1.30e-22 89.4
MS.gene006980.t1 MTR_2g014760 40.000 100 58 2 4 102 29 127 4.06e-22 84.0
MS.gene006980.t1 MTR_2g048970 40.952 105 58 1 2 102 29 133 9.81e-22 83.2
MS.gene006980.t1 MTR_4g079110 41.837 98 56 1 6 102 58 155 8.77e-21 81.3
MS.gene006980.t1 MTR_2g090755 36.000 100 63 1 4 102 41 140 8.47e-20 78.6
MS.gene006980.t1 MTR_3g077570 37.000 100 63 0 1 100 18 117 1.28e-18 74.7
MS.gene006980.t1 MTR_3g077560 35.000 100 65 0 1 100 21 120 6.32e-17 70.9
MS.gene006980.t1 MTR_1g015890 36.634 101 62 1 4 102 49 149 1.79e-16 70.1
MS.gene006980.t1 MTR_5g077550 35.922 103 64 1 2 102 21 123 1.19e-15 67.4
MS.gene006980.t1 MTR_7g035245 39.362 94 57 0 7 100 9 102 5.16e-15 65.5
MS.gene006980.t1 MTR_1g069255 36.364 99 62 1 2 99 84 182 1.89e-14 65.9
MS.gene006980.t1 MTR_3g104510 33.333 99 60 3 4 101 24 117 4.99e-13 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006980.t1 AT3G62930 63.725 102 37 0 1 102 1 102 1.53e-47 147
MS.gene006980.t1 AT1G03020 57.843 102 43 0 1 102 1 102 9.25e-45 140
MS.gene006980.t1 AT5G18600 56.863 102 44 0 1 102 1 102 3.93e-44 139
MS.gene006980.t1 AT4G15680 53.922 102 47 0 1 102 1 102 1.31e-40 130
MS.gene006980.t1 AT4G15690 53.922 102 47 0 1 102 1 102 1.90e-40 129
MS.gene006980.t1 AT4G15700 52.941 102 48 0 1 102 1 102 3.63e-40 129
MS.gene006980.t1 AT4G15670 50.000 102 51 0 1 102 1 102 3.50e-38 124
MS.gene006980.t1 AT4G15660 50.000 102 51 0 1 102 1 102 4.32e-38 123
MS.gene006980.t1 AT3G21460 44.118 102 57 0 1 102 1 102 7.71e-31 105
MS.gene006980.t1 AT3G62950 43.689 103 57 1 1 102 1 103 2.22e-28 99.4
MS.gene006980.t1 AT2G47870 42.718 103 58 1 1 102 1 103 1.00e-27 97.4
MS.gene006980.t1 AT1G06830 46.316 95 51 0 1 95 1 95 2.50e-27 96.3
MS.gene006980.t1 AT2G30540 45.263 95 52 0 1 95 1 95 9.16e-27 95.1
MS.gene006980.t1 AT5G14070 42.056 107 54 2 4 102 34 140 4.40e-26 94.4
MS.gene006980.t1 AT2G47880 42.574 101 58 0 1 101 1 101 5.09e-26 93.2
MS.gene006980.t1 AT3G62960 42.105 95 55 0 1 95 1 95 4.39e-24 88.2
MS.gene006980.t1 AT3G02000 39.048 105 58 1 4 102 32 136 8.36e-22 83.6
MS.gene006980.t1 AT1G28480 33.019 106 65 2 2 102 33 137 1.11e-18 75.5
MS.gene006980.t1 AT4G33040 36.893 103 61 1 4 102 42 144 5.09e-17 71.6
MS.gene006980.t1 AT5G63030 36.000 100 64 0 1 100 19 118 1.06e-16 70.1
MS.gene006980.t1 AT5G40370 39.000 100 61 0 1 100 3 102 1.59e-16 69.3
MS.gene006980.t1 AT2G20270 37.374 99 61 1 2 99 76 174 1.61e-16 70.9
MS.gene006980.t1 AT1G03850 34.694 98 56 2 7 102 59 150 7.86e-16 68.6
MS.gene006980.t1 AT5G11930 32.292 96 55 3 7 101 61 147 1.95e-14 64.7
MS.gene006980.t1 AT5G40370 40.698 86 51 0 15 100 42 127 5.64e-13 60.8

Find 0 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTTGATAAAATCACAAAT+GGG - 51716:34739-34758 MS.gene006980:CDS 20.0%
!! TATCAAGTTCATAAACAATT+GGG + 51716:34728-34747 None:intergenic 20.0%
!! TTATCAAGTTCATAAACAAT+TGG + 51716:34729-34748 None:intergenic 20.0%
! GAACTTGATAAAATCACAAA+TGG - 51716:34738-34757 MS.gene006980:CDS 25.0%
AAGTTCATAAACAATTGGGT+TGG + 51716:34724-34743 None:intergenic 30.0%
TCAATTAGTACCTATGTTGA+AGG - 51716:34878-34897 MS.gene006980:CDS 30.0%
!!! ATGAAGGCTCATGATTTTTT+TGG + 51716:34850-34869 None:intergenic 30.0%
AAAGTGAGTTGCTTCAAATG+GGG - 51716:34772-34791 MS.gene006980:CDS 35.0%
GAAAGTGAGTTGCTTCAAAT+GGG - 51716:34771-34790 MS.gene006980:CDS 35.0%
TGAAAGTGAGTTGCTTCAAA+TGG - 51716:34770-34789 MS.gene006980:CDS 35.0%
TTCATAGGACAACAATTCAG+AGG - 51716:34819-34838 MS.gene006980:CDS 35.0%
! CTAATTGATTCCTCACATGA+AGG + 51716:34866-34885 None:intergenic 35.0%
AAATCATGAGCCTTCATGTG+AGG - 51716:34853-34872 MS.gene006980:CDS 40.0%
ACATCACTGATACGTAGCTT+TGG - 51716:34696-34715 MS.gene006980:CDS 40.0%
ATAGGACAACAATTCAGAGG+TGG - 51716:34822-34841 MS.gene006980:CDS 40.0%
ATGAAAACAGCAGGTACACT+TGG + 51716:34804-34823 None:intergenic 40.0%
GTATCAGTGATGTTACAGAG+TGG + 51716:34689-34708 None:intergenic 40.0%
TGTTGTCCTATGAAAACAGC+AGG + 51716:34813-34832 None:intergenic 40.0%
!!! AGTGTACCTGCTGTTTTCAT+AGG - 51716:34804-34823 MS.gene006980:CDS 40.0%
CTCTTGCTGAATATCACCAC+TGG + 51716:34651-34670 None:intergenic 45.0%
GTACCTATGTTGAAGGATGC+TGG - 51716:34885-34904 MS.gene006980:CDS 45.0%
TGAAGGATGCTGGAGCTATA+TGG - 51716:34895-34914 MS.gene006980:CDS 45.0%
GACACAGTCACAAGCATGGT+TGG - 51716:34618-34637 MS.gene006980:CDS 50.0%
GCTCCAGCATCCTTCAACAT+AGG + 51716:34891-34910 None:intergenic 50.0%
!! CATGGTTGGTGAAAAGCCAG+TGG - 51716:34632-34651 MS.gene006980:CDS 50.0%


Chromosome Type Strat End Strand Name
51716 gene 34615 34923 34615 ID=MS.gene006980
51716 mRNA 34615 34923 34615 ID=MS.gene006980.t1;Parent=MS.gene006980
51716 exon 34615 34923 34615 ID=MS.gene006980.t1.exon1;Parent=MS.gene006980.t1
51716 CDS 34615 34923 34615 ID=cds.MS.gene006980.t1;Parent=MS.gene006980.t1
Gene Sequence

>MS.gene006980

ATGGACACAGTCACAAGCATGGTTGGTGAAAAGCCAGTGGTGATATTCAGCAAGAGTACGTGTTGCTTGAGCCACTCTGTAACATCACTGATACGTAGCTTTGGAGCCAACCCAATTGTTTATGAACTTGATAAAATCACAAATGGGTCACAGATTGAAAGTGAGTTGCTTCAAATGGGGTGTAAACCAAGTGTACCTGCTGTTTTCATAGGACAACAATTCAGAGGTGGTTCCAAAAAAATCATGAGCCTTCATGTGAGGAATCAATTAGTACCTATGTTGAAGGATGCTGGAGCTATATGGATTTGA

Protein sequence

>MS.gene006980.t1

MDTVTSMVGEKPVVIFSKSTCCLSHSVTSLIRSFGANPIVYELDKITNGSQIESELLQMGCKPSVPAVFIGQQFRGGSKKIMSLHVRNQLVPMLKDAGAIWI