AlfalfaGEDB | MS.gene20702

M. sativa cultivar XinJiangDaYe / MS.gene20702

KEGG: K03676
GO: GO:0003674 GO:0003824 GO:0008150 GO:0009055 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0019725 GO:0042592 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008
PFAM: PF00462.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20702.t1	XP_013469214.1	99	99	1	0	1	99	1	99	3.20E-48	200.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20702.t1	Q9M9Y9	75.3	97	24	0	1	97	1	97	2.0e-38	159.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20702.t1	A0A072VN37	99.0	99	1	0	1	99	1	99	2.3e-48	200.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055217	MS.gene20702	0.803457	3.45E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene20702	MS.gene050229	PPI
MS.gene022312	MS.gene20702	PPI
MS.gene70642	MS.gene20702	PPI
MS.gene20702	MS.gene041521	PPI
MS.gene20702	MS.gene07181	PPI
MS.gene20702	MS.gene23008	PPI
MS.gene005632	MS.gene20702	PPI
MS.gene20702	MS.gene07180	PPI
MS.gene07180	MS.gene20702	PPI
MS.gene20702	MS.gene20705	PPI
MS.gene20702	MS.gene036527	PPI
MS.gene20702	MS.gene005628	PPI
MS.gene20702	MS.gene005630	PPI
MS.gene23005	MS.gene20702	PPI
MS.gene20702	MS.gene035294	PPI
MS.gene29371	MS.gene20702	PPI
MS.gene03023	MS.gene20702	PPI
MS.gene20702	MS.gene52509	PPI
MS.gene20702	MS.gene20704	PPI
MS.gene20702	MS.gene03024	PPI
MS.gene20702	MS.gene005629	PPI
MS.gene20702	MS.gene07183	PPI
MS.gene20702	MS.gene03022	PPI
MS.gene58935	MS.gene20702	PPI
MS.gene20702	MS.gene58937	PPI
MS.gene20702	MS.gene23009	PPI
MS.gene20702	MS.gene041519	PPI
MS.gene20702	MS.gene87167	PPI
MS.gene20702	MS.gene25978	PPI
MS.gene20702	MS.gene041518	PPI
MS.gene036528	MS.gene20702	PPI
MS.gene20702	MS.gene59643	PPI
MS.gene006983	MS.gene20702	PPI
MS.gene20702	MS.gene035291	PPI
MS.gene006980	MS.gene20702	PPI
MS.gene035290	MS.gene20702	PPI
MS.gene20702	MS.gene58938	PPI
MS.gene005633	MS.gene20702	PPI
MS.gene20702	MS.gene005631	PPI
MS.gene20702	MS.gene29979	PPI
MS.gene20702	MS.gene022311	PPI
MS.gene20702	MS.gene70641	PPI
MS.gene20703	MS.gene20702	PPI
MS.gene07179	MS.gene20702	PPI
MS.gene20702	MS.gene006984	PPI
MS.gene20702	MS.gene07179	PPI
MS.gene20702	MS.gene20701	PPI
MS.gene20702	MS.gene07182	PPI
MS.gene07183	MS.gene20702	PPI
MS.gene20702	MS.gene03026	PPI
MS.gene25976	MS.gene20702	PPI
MS.gene20702	MS.gene29370	PPI
MS.gene23007	MS.gene20702	PPI
MS.gene20702	MS.gene006982	PPI
MS.gene036524	MS.gene20702	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20702.t1	MTR_1g088920	98.990	99	1	0	1	99	1	99	6.71e-69	201
MS.gene20702.t1	MTR_1g088925	95.960	99	4	0	1	99	1	99	5.12e-68	199
MS.gene20702.t1	MTR_7g108200	79.592	98	20	0	1	98	1	98	4.02e-58	174
MS.gene20702.t1	MTR_2g019900	58.947	95	39	0	1	95	1	95	2.67e-39	126
MS.gene20702.t1	MTR_1g088910	55.102	98	44	0	1	98	1	98	6.05e-37	120
MS.gene20702.t1	MTR_2g019950	55.789	95	42	0	1	95	1	95	2.51e-36	119
MS.gene20702.t1	MTR_7g022690	54.167	96	43	1	1	95	30	125	7.68e-35	116
MS.gene20702.t1	MTR_7g022710	53.125	96	44	1	1	95	30	125	2.42e-34	115
MS.gene20702.t1	MTR_7g022550	53.125	96	44	1	1	95	30	125	2.25e-33	112
MS.gene20702.t1	MTR_4g119050	52.083	96	45	1	1	95	1	96	2.08e-32	109
MS.gene20702.t1	MTR_4g119030	52.083	96	45	1	1	95	1	96	2.08e-32	109
MS.gene20702.t1	MTR_4g119040	52.083	96	45	1	1	95	1	96	2.08e-32	109
MS.gene20702.t1	MTR_7g026770	54.167	96	43	1	1	95	22	117	5.64e-32	113
MS.gene20702.t1	MTR_7g108220	52.632	95	44	1	1	94	1	95	1.63e-31	107
MS.gene20702.t1	MTR_7g108210	51.579	95	45	1	1	94	1	95	1.12e-30	104
MS.gene20702.t1	MTR_7g108250	43.158	95	54	0	1	95	1	95	1.16e-25	92.0
MS.gene20702.t1	MTR_1g088905	40.426	94	56	0	1	94	1	94	3.99e-25	90.9
MS.gene20702.t1	MTR_1g088895	44.898	98	54	0	1	98	1	98	2.10e-21	81.6
MS.gene20702.t1	MTR_2g048970	41.837	98	53	1	2	95	29	126	6.13e-21	80.9
MS.gene20702.t1	MTR_2g014760	40.426	94	56	0	2	95	27	120	5.44e-20	78.6
MS.gene20702.t1	MTR_7g108260	41.489	94	55	0	1	94	1	94	6.07e-20	77.8
MS.gene20702.t1	MTR_2g090755	36.458	96	54	2	4	95	41	133	1.93e-18	74.7
MS.gene20702.t1	MTR_3g077570	35.789	95	61	0	1	95	18	112	3.28e-16	68.6
MS.gene20702.t1	MTR_4g079110	38.710	93	52	3	6	95	58	148	9.40e-16	68.2
MS.gene20702.t1	MTR_3g077560	34.043	94	62	0	1	94	21	114	1.35e-15	67.0
MS.gene20702.t1	MTR_7g035245	35.106	94	61	0	1	94	3	96	8.27e-15	64.7
MS.gene20702.t1	MTR_1g015890	38.298	94	56	1	3	94	48	141	2.21e-14	64.7
MS.gene20702.t1	MTR_1g069255	32.979	94	62	1	2	94	84	177	6.47e-14	64.3
MS.gene20702.t1	MTR_3g104510	37.634	93	52	3	3	94	23	110	3.88e-13	60.5
MS.gene20702.t1	MTR_2g038560	30.108	93	65	0	2	94	29	121	9.22e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20702.t1	AT1G06830	75.258	97	24	0	1	97	1	97	1.50e-52	160
MS.gene20702.t1	AT2G30540	70.408	98	29	0	1	98	1	98	4.04e-52	159
MS.gene20702.t1	AT2G47880	72.449	98	27	0	1	98	1	98	5.19e-52	158
MS.gene20702.t1	AT3G62960	70.408	98	29	0	1	98	1	98	2.03e-50	154
MS.gene20702.t1	AT3G62950	51.042	96	46	1	1	95	1	96	2.13e-36	119
MS.gene20702.t1	AT2G47870	53.125	96	44	1	1	95	1	96	9.34e-36	117
MS.gene20702.t1	AT4G15670	50.526	95	47	0	1	95	1	95	3.26e-34	114
MS.gene20702.t1	AT3G21460	54.737	95	43	0	1	95	1	95	4.10e-34	113
MS.gene20702.t1	AT4G15680	51.579	95	46	0	1	95	1	95	1.11e-33	112
MS.gene20702.t1	AT4G15660	49.485	97	49	0	1	97	1	97	1.12e-33	112
MS.gene20702.t1	AT4G15700	51.579	95	46	0	1	95	1	95	1.25e-33	112
MS.gene20702.t1	AT4G15690	50.526	95	47	0	1	95	1	95	1.10e-32	110
MS.gene20702.t1	AT5G18600	48.421	95	49	0	1	95	1	95	1.57e-31	107
MS.gene20702.t1	AT5G14070	45.631	103	48	2	1	95	31	133	1.26e-26	95.9
MS.gene20702.t1	AT3G02000	45.455	99	48	1	3	95	31	129	2.50e-25	92.4
MS.gene20702.t1	AT3G62930	43.158	95	54	0	1	95	1	95	2.82e-25	91.3
MS.gene20702.t1	AT1G03020	45.263	95	52	0	1	95	1	95	6.76e-25	90.1
MS.gene20702.t1	AT4G33040	42.268	97	50	2	3	94	41	136	1.55e-17	72.8
MS.gene20702.t1	AT1G28480	36.735	98	58	2	2	95	33	130	2.53e-17	72.0
MS.gene20702.t1	AT5G40370	32.979	94	63	0	1	94	3	96	9.74e-15	64.7
MS.gene20702.t1	AT5G11930	41.935	93	44	3	3	94	57	140	1.49e-14	65.1
MS.gene20702.t1	AT4G28730	35.106	94	60	1	2	94	71	164	1.60e-14	65.5
MS.gene20702.t1	AT5G63030	36.585	82	52	0	13	94	31	112	2.59e-12	58.9
MS.gene20702.t1	AT2G20270	34.043	94	61	1	2	94	76	169	6.60e-12	58.9
MS.gene20702.t1	AT5G20500	31.868	91	62	0	4	94	35	125	1.44e-11	57.0
MS.gene20702.t1	AT4G28730	37.143	70	43	1	2	70	71	140	8.26e-11	55.1
MS.gene20702.t1	AT1G03850	34.884	86	52	2	10	95	62	143	9.42e-11	55.1

Find 33 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATTCTGTTTCAAGAAAT+TGG	0.120000	1.2:+66677832	MS.gene20702:CDS
AGATCCTGAAGGAAAAGAAA+TGG	0.394593	1.2:+66677885	MS.gene20702:CDS
GTGGAGGGACATTACTTCAT+TGG	0.411453	1.2:-66677983	None:intergenic
GGAGAAAGCTATAACAAAGT+TGG	0.418322	1.2:+66677906	MS.gene20702:CDS
CCTGTCCCTGCAGTATTCAT+TGG	0.431206	1.2:+66677940	MS.gene20702:CDS
GAGAAAGCTATAACAAAGTT+GGG	0.448427	1.2:+66677907	MS.gene20702:CDS
GATGAGGTTGGCTACAGAAA+AGG	0.457049	1.2:+66677762	MS.gene20702:CDS
TTCATTGGAGGAAAGCTTGT+GGG	0.469806	1.2:+66677955	MS.gene20702:CDS
TTTCCTCCAATGAATACTGC+AGG	0.495376	1.2:-66677946	None:intergenic
TTGTCAATTTCATGAACCAC+AGG	0.499275	1.2:-66677862	None:intergenic
TGATCAAATTAAGCTAAATA+CGG	0.503808	1.2:-66678041	None:intergenic
ATGAGGTTGGCTACAGAAAA+GGG	0.514459	1.2:+66677763	MS.gene20702:CDS
GATGGAGAAGGTGATGAGGT+TGG	0.515048	1.2:+66677750	None:intergenic
GCACAAACAACAAGAGCTCT+TGG	0.517591	1.2:-66677800	None:intergenic
CCAATGAATACTGCAGGGAC+AGG	0.522380	1.2:-66677940	None:intergenic
GAGGGACATTACTTCATTGG+TGG	0.543110	1.2:-66677980	None:intergenic
GTCCCTGCAGTATTCATTGG+AGG	0.543693	1.2:+66677943	MS.gene20702:CDS
AAATTAAGCTAAATACGGTA+TGG	0.562707	1.2:-66678036	None:intergenic
CGAGTGAACCACTTAGGTGG+AGG	0.574745	1.2:-66677999	None:intergenic
ATACGGTATGGCCTAAGTAG+TGG	0.575235	1.2:-66678024	None:intergenic
GTAATGTCCCTCCACCTAAG+TGG	0.591093	1.2:+66677991	MS.gene20702:CDS
GTTCACTCGTTCCACTACTT+AGG	0.591318	1.2:+66678013	MS.gene20702:CDS
GTGGAACGAGTGAACCACTT+AGG	0.595515	1.2:-66678005	None:intergenic
CAGTTATATTAAGATGGAGA+AGG	0.604195	1.2:+66677738	None:intergenic
TCATTGGAGGAAAGCTTGTG+GGG	0.613505	1.2:+66677956	MS.gene20702:CDS
GAAATTGACAAAGATCCTGA+AGG	0.616874	1.2:+66677874	MS.gene20702:CDS
ATTCATTGGAGGAAAGCTTG+TGG	0.621609	1.2:+66677954	MS.gene20702:CDS
GAACGAGTGAACCACTTAGG+TGG	0.634239	1.2:-66678002	None:intergenic
TTCCTCCAATGAATACTGCA+GGG	0.637919	1.2:-66677945	None:intergenic
GAGTGAACCACTTAGGTGGA+GGG	0.652910	1.2:-66677998	None:intergenic
TTAAGATGGAGAAGGTGATG+AGG	0.668317	1.2:+66677746	None:intergenic
TGTCAATTTCATGAACCACA+GGG	0.678104	1.2:-66677861	None:intergenic
AGAAATTGGAGTGTTCCCTG+TGG	0.708961	1.2:+66677846	MS.gene20702:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AACATTCTGTTTCAAGAAAT+TGG	+	chr1.2:66677832-66677851	MS.gene20702:CDS	25.0%
	GAGAAAGCTATAACAAAGTT+GGG	+	chr1.2:66677907-66677926	MS.gene20702:CDS	30.0%
	AGATCCTGAAGGAAAAGAAA+TGG	+	chr1.2:66677885-66677904	MS.gene20702:CDS	35.0%
	GAAATTGACAAAGATCCTGA+AGG	+	chr1.2:66677874-66677893	MS.gene20702:CDS	35.0%
	GGAGAAAGCTATAACAAAGT+TGG	+	chr1.2:66677906-66677925	MS.gene20702:CDS	35.0%
	TGTCAATTTCATGAACCACA+GGG	-	chr1.2:66677864-66677883	None:intergenic	35.0%
	TTGTCAATTTCATGAACCAC+AGG	-	chr1.2:66677865-66677884	None:intergenic	35.0%
!	TTCTCCATTTCTTTTCCTTC+AGG	-	chr1.2:66677892-66677911	None:intergenic	35.0%
	ATTCATTGGAGGAAAGCTTG+TGG	+	chr1.2:66677954-66677973	MS.gene20702:CDS	40.0%
	TTCATTGGAGGAAAGCTTGT+GGG	+	chr1.2:66677955-66677974	MS.gene20702:CDS	40.0%
	TTCCTCCAATGAATACTGCA+GGG	-	chr1.2:66677948-66677967	None:intergenic	40.0%
	TTTCCTCCAATGAATACTGC+AGG	-	chr1.2:66677949-66677968	None:intergenic	40.0%
!	ATGAGGTTGGCTACAGAAAA+GGG	+	chr1.2:66677763-66677782	MS.gene20702:CDS	40.0%
	ATACGGTATGGCCTAAGTAG+TGG	-	chr1.2:66678027-66678046	None:intergenic	45.0%
	GAGGGACATTACTTCATTGG+TGG	-	chr1.2:66677983-66678002	None:intergenic	45.0%
	GCACAAACAACAAGAGCTCT+TGG	-	chr1.2:66677803-66677822	None:intergenic	45.0%
	GTGGAGGGACATTACTTCAT+TGG	-	chr1.2:66677986-66678005	None:intergenic	45.0%
	GTTCACTCGTTCCACTACTT+AGG	+	chr1.2:66678013-66678032	MS.gene20702:CDS	45.0%
	TCATTGGAGGAAAGCTTGTG+GGG	+	chr1.2:66677956-66677975	MS.gene20702:CDS	45.0%
!	AGAAATTGGAGTGTTCCCTG+TGG	+	chr1.2:66677846-66677865	MS.gene20702:CDS	45.0%
!	GATGAGGTTGGCTACAGAAA+AGG	+	chr1.2:66677762-66677781	MS.gene20702:CDS	45.0%
	CCAATGAATACTGCAGGGAC+AGG	-	chr1.2:66677943-66677962	None:intergenic	50.0%
	CCTGTCCCTGCAGTATTCAT+TGG	+	chr1.2:66677940-66677959	MS.gene20702:CDS	50.0%
	GAACGAGTGAACCACTTAGG+TGG	-	chr1.2:66678005-66678024	None:intergenic	50.0%
	GAGTGAACCACTTAGGTGGA+GGG	-	chr1.2:66678001-66678020	None:intergenic	50.0%
	GTAATGTCCCTCCACCTAAG+TGG	+	chr1.2:66677991-66678010	MS.gene20702:CDS	50.0%
	GTCCCTGCAGTATTCATTGG+AGG	+	chr1.2:66677943-66677962	MS.gene20702:CDS	50.0%
	GTGGAACGAGTGAACCACTT+AGG	-	chr1.2:66678008-66678027	None:intergenic	50.0%
	CGAGTGAACCACTTAGGTGG+AGG	-	chr1.2:66678002-66678021	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	66677751	66678050	66677751	ID=MS.gene20702
chr1.2	mRNA	66677751	66678050	66677751	ID=MS.gene20702.t1;Parent=MS.gene20702
chr1.2	exon	66677751	66678050	66677751	ID=MS.gene20702.t1.exon1;Parent=MS.gene20702.t1
chr1.2	CDS	66677751	66678050	66677751	ID=cds.MS.gene20702.t1;Parent=MS.gene20702.t1

Gene Sequence	>MS.gene20702 ATGGAGAAGGTGATGAGGTTGGCTACAGAAAAGGGTGTTGTGATATTCACCAAGAGCTCTTGTTGTTTGTGCTATGCAGTGAACATTCTGTTTCAAGAAATTGGAGTGTTCCCTGTGGTTCATGAAATTGACAAAGATCCTGAAGGAAAAGAAATGGAGAAAGCTATAACAAAGTTGGGTTGTAATGCACCTGTCCCTGCAGTATTCATTGGAGGAAAGCTTGTGGGGTCCACCAATGAAGTAATGTCCCTCCACCTAAGTGGTTCACTCGTTCCACTACTTAGGCCATACCGTATTTAG
Protein sequence	>MS.gene20702.t1 MEKVMRLATEKGVVIFTKSSCCLCYAVNILFQEIGVFPVVHEIDKDPEGKEMEKAITKLGCNAPVPAVFIGGKLVGSTNEVMSLHLSGSLVPLLRPYRI