AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006984

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006984.t1 MTR_7g108200 99.020 102 1 0 1 102 1 102 1.51e-72 210
MS.gene006984.t1 MTR_1g088920 78.571 98 21 0 1 98 1 98 2.48e-57 172
MS.gene006984.t1 MTR_1g088925 76.531 98 23 0 1 98 1 98 3.56e-57 171
MS.gene006984.t1 MTR_1g088910 57.447 94 40 0 1 94 1 94 2.57e-37 121
MS.gene006984.t1 MTR_2g019900 55.789 95 42 0 1 95 1 95 3.13e-37 121
MS.gene006984.t1 MTR_2g019950 55.789 95 42 0 1 95 1 95 5.90e-37 120
MS.gene006984.t1 MTR_7g108220 53.684 95 43 1 1 94 1 95 1.54e-32 109
MS.gene006984.t1 MTR_7g022690 46.875 96 50 1 1 95 30 125 3.84e-32 109
MS.gene006984.t1 MTR_7g108210 52.632 95 44 1 1 94 1 95 1.05e-31 107
MS.gene006984.t1 MTR_7g022710 46.875 96 50 1 1 95 30 125 1.05e-31 108
MS.gene006984.t1 MTR_4g119050 50.000 96 47 1 1 95 1 96 1.75e-31 107
MS.gene006984.t1 MTR_4g119030 50.000 96 47 1 1 95 1 96 1.75e-31 107
MS.gene006984.t1 MTR_4g119040 50.000 96 47 1 1 95 1 96 1.75e-31 107
MS.gene006984.t1 MTR_7g022550 45.833 96 51 1 1 95 30 125 6.78e-31 106
MS.gene006984.t1 MTR_7g026770 50.000 96 47 1 1 95 22 117 1.34e-30 110
MS.gene006984.t1 MTR_7g108250 45.263 95 52 0 1 95 1 95 3.91e-26 93.6
MS.gene006984.t1 MTR_1g088905 42.553 94 54 0 1 94 1 94 1.02e-24 89.7
MS.gene006984.t1 MTR_1g088895 44.681 94 52 0 1 94 1 94 6.68e-22 82.8
MS.gene006984.t1 MTR_7g108260 42.553 94 54 0 1 94 1 94 1.67e-21 81.6
MS.gene006984.t1 MTR_2g014760 38.298 94 58 0 2 95 27 120 8.30e-19 75.5
MS.gene006984.t1 MTR_2g048970 38.776 98 56 1 2 95 29 126 1.21e-17 72.8
MS.gene006984.t1 MTR_2g090755 34.375 96 56 2 4 95 41 133 1.75e-16 70.1
MS.gene006984.t1 MTR_1g069255 36.170 94 59 1 2 94 84 177 1.58e-15 68.6
MS.gene006984.t1 MTR_4g079110 36.082 97 59 2 2 95 52 148 9.10e-15 65.9
MS.gene006984.t1 MTR_3g077570 32.979 94 63 0 1 94 18 111 1.80e-13 61.6
MS.gene006984.t1 MTR_7g035245 34.043 94 62 0 1 94 3 96 4.42e-13 60.5
MS.gene006984.t1 MTR_3g077560 32.979 94 63 0 1 94 21 114 1.74e-12 59.3
MS.gene006984.t1 MTR_1g015890 34.737 95 52 2 3 91 48 138 2.95e-12 59.3
MS.gene006984.t1 MTR_2g038560 31.707 82 56 0 13 94 40 121 3.97e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006984.t1 AT2G47880 75.758 99 24 0 1 99 1 99 1.14e-54 165
MS.gene006984.t1 AT2G30540 71.000 100 29 0 1 100 1 100 4.86e-54 164
MS.gene006984.t1 AT1G06830 74.227 97 25 0 1 97 1 97 2.59e-53 162
MS.gene006984.t1 AT3G62960 71.569 102 29 0 1 102 1 102 4.92e-53 161
MS.gene006984.t1 AT3G62950 53.000 100 46 1 1 99 1 100 1.01e-37 122
MS.gene006984.t1 AT2G47870 53.125 96 44 1 1 95 1 96 4.10e-36 118
MS.gene006984.t1 AT3G21460 54.737 95 43 0 1 95 1 95 1.93e-35 117
MS.gene006984.t1 AT4G15680 49.474 95 48 0 1 95 1 95 1.27e-32 109
MS.gene006984.t1 AT5G18600 50.526 95 47 0 1 95 1 95 2.30e-32 109
MS.gene006984.t1 AT4G15670 48.421 95 49 0 1 95 1 95 5.52e-32 108
MS.gene006984.t1 AT4G15660 47.423 97 51 0 1 97 1 97 9.85e-32 107
MS.gene006984.t1 AT4G15700 48.421 95 49 0 1 95 1 95 1.43e-31 107
MS.gene006984.t1 AT4G15690 48.421 95 49 0 1 95 1 95 2.67e-31 106
MS.gene006984.t1 AT5G14070 43.689 103 50 2 1 95 31 133 3.50e-26 94.7
MS.gene006984.t1 AT3G62930 42.105 95 55 0 1 95 1 95 2.48e-25 91.3
MS.gene006984.t1 AT3G02000 43.434 99 50 1 3 95 31 129 1.23e-23 88.2
MS.gene006984.t1 AT1G03020 42.105 95 55 0 1 95 1 95 1.83e-23 86.7
MS.gene006984.t1 AT1G28480 33.673 98 61 2 2 95 33 130 3.93e-15 66.2
MS.gene006984.t1 AT4G33040 39.785 93 50 2 3 90 41 132 5.21e-15 66.2
MS.gene006984.t1 AT4G28730 35.556 90 57 1 2 90 71 160 1.08e-14 66.2
MS.gene006984.t1 AT5G40370 31.915 94 64 0 1 94 3 96 1.28e-12 59.3
MS.gene006984.t1 AT5G20500 33.333 93 62 0 2 94 33 125 4.71e-12 58.5
MS.gene006984.t1 AT4G28730 38.571 70 42 1 2 70 71 140 1.12e-11 57.8
MS.gene006984.t1 AT2G20270 31.915 94 63 1 2 94 76 169 1.62e-11 57.8

Find 0 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACATTCTGTTTCAAGAACT+TGG + 51716:56593-56612 MS.gene006984:CDS 30.0%
ACATTCTGTTTCAAGAACTT+GGG + 51716:56594-56613 MS.gene006984:CDS 30.0%
TGATCAATTTCATGAACCAT+AGG - 51716:56626-56645 None:intergenic 30.0%
AAGGGACATAATTTCATTGG+TGG - 51716:56744-56763 None:intergenic 35.0%
CAATTAGAACGAGATTGGTA+TGG - 51716:56800-56819 None:intergenic 35.0%
GTGAAGGGACATAATTTCAT+TGG - 51716:56747-56766 None:intergenic 35.0%
TGTTTCAAGAACTTGGGATT+AGG + 51716:56600-56619 MS.gene006984:CDS 35.0%
!! AAATGGAGAAAGCTTTGTTG+AGG + 51716:56663-56682 MS.gene006984:CDS 35.0%
ATGGCTTGATCAATTGAGTG+AGG - 51716:56781-56800 None:intergenic 40.0%
ATTATGTCCCTTCACCTAAG+TGG + 51716:56752-56771 MS.gene006984:CDS 40.0%
GAAATTGATCAAGACCCTGA+AGG + 51716:56635-56654 MS.gene006984:CDS 40.0%
TGGCTTGATCAATTGAGTGA+GGG - 51716:56780-56799 None:intergenic 40.0%
TTGAGGTTGGCATCTGAAAA+AGG + 51716:56524-56543 MS.gene006984:CDS 40.0%
!! GAGAAAGCTTTGTTGAGGTT+AGG + 51716:56668-56687 MS.gene006984:CDS 40.0%
AGAACTTGGGATTAGGCCTA+TGG + 51716:56607-56626 MS.gene006984:CDS 45.0%
GTTGGCATCTGAAAAAGGTG+TGG + 51716:56529-56548 MS.gene006984:CDS 45.0%
TCTCCATTTCCCTTCCTTCA+GGG - 51716:56652-56671 None:intergenic 45.0%
TGATCAAGACCCTGAAGGAA+GGG + 51716:56640-56659 MS.gene006984:CDS 45.0%
TTCATTGGAGGGCAACTGAT+GGG + 51716:56716-56735 MS.gene006984:CDS 45.0%
TTCCTGCTGTGTTCATTGGA+GGG + 51716:56705-56724 MS.gene006984:CDS 45.0%
TTCTCCATTTCCCTTCCTTC+AGG - 51716:56653-56672 None:intergenic 45.0%
TTGATCAAGACCCTGAAGGA+AGG + 51716:56639-56658 MS.gene006984:CDS 45.0%
AGACCCTGAAGGAAGGGAAA+TGG + 51716:56646-56665 MS.gene006984:CDS 50.0%
ATTGAGTGAGGGAGCCACTT+AGG - 51716:56769-56788 None:intergenic 50.0%
CCAATGAACACAGCAGGAAC+AGG - 51716:56704-56723 None:intergenic 50.0%
CCTGTTCCTGCTGTGTTCAT+TGG + 51716:56701-56720 MS.gene006984:CDS 50.0%
GTTCATTGGAGGGCAACTGA+TGG + 51716:56715-56734 MS.gene006984:CDS 50.0%
GTTCCTGCTGTGTTCATTGG+AGG + 51716:56704-56723 MS.gene006984:CDS 50.0%
TCATTGGAGGGCAACTGATG+GGG + 51716:56717-56736 MS.gene006984:CDS 50.0%
GAGGGAGCCACTTAGGTGAA+GGG - 51716:56762-56781 None:intergenic 55.0%
TGAGGGAGCCACTTAGGTGA+AGG - 51716:56763-56782 None:intergenic 55.0%
TGCCCTCCAATGAACACAGC+AGG - 51716:56710-56729 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
51716 gene 56512 56820 56512 ID=MS.gene006984
51716 mRNA 56512 56820 56512 ID=MS.gene006984.t1;Parent=MS.gene006984
51716 exon 56512 56820 56512 ID=MS.gene006984.t1.exon1;Parent=MS.gene006984.t1
51716 CDS 56512 56820 56512 ID=cds.MS.gene006984.t1;Parent=MS.gene006984.t1
Gene Sequence

>MS.gene006984

ATGGACAAAGTGTTGAGGTTGGCATCTGAAAAAGGTGTGGTGATTTTCACAAAGAGTTCATGTTGTCTATGCTATGCAGTTAACATTCTGTTTCAAGAACTTGGGATTAGGCCTATGGTTCATGAAATTGATCAAGACCCTGAAGGAAGGGAAATGGAGAAAGCTTTGTTGAGGTTAGGTTGTACTGCACCTGTTCCTGCTGTGTTCATTGGAGGGCAACTGATGGGGTCCACCAATGAAATTATGTCCCTTCACCTAAGTGGCTCCCTCACTCAATTGATCAAGCCATACCAATCTCGTTCTAATTGA
Protein sequence

>MS.gene006984.t1

MDKVLRLASEKGVVIFTKSSCCLCYAVNILFQELGIRPMVHEIDQDPEGREMEKALLRLGCTAPVPAVFIGGQLMGSTNEIMSLHLSGSLTQLIKPYQSRSN