AlfalfaGEDB | MS.gene07181

M. sativa cultivar XinJiangDaYe / MS.gene07181

KEGG: K03676
GO: GO:0003674 GO:0003824 GO:0008150 GO:0009055 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0019725 GO:0042592 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008
PFAM: PF00462.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07181.t1	PNX99618.1	100	102	0	0	1	102	52	153	1.20E-50	208.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07181.t1	Q9LYC6	75.5	102	24	1	1	101	1	102	8.3e-40	164.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07181.t1	B7FMA1	100.0	102	0	0	1	102	1	102	8.8e-51	208.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene005629	MS.gene07181	PPI
MS.gene03024	MS.gene07181	PPI
MS.gene07181	MS.gene20701	PPI
MS.gene005633	MS.gene07181	PPI
MS.gene29979	MS.gene07181	PPI
MS.gene20701	MS.gene07181	PPI
MS.gene006984	MS.gene07181	PPI
MS.gene20702	MS.gene07181	PPI
MS.gene20703	MS.gene07181	PPI
MS.gene07181	MS.gene006982	PPI
MS.gene07183	MS.gene07181	PPI
MS.gene25976	MS.gene07181	PPI
MS.gene041521	MS.gene07181	PPI
MS.gene006982	MS.gene07181	PPI
MS.gene23007	MS.gene07181	PPI
MS.gene58938	MS.gene07181	PPI
MS.gene23008	MS.gene07181	PPI
MS.gene036524	MS.gene07181	PPI
MS.gene03022	MS.gene07181	PPI
MS.gene022312	MS.gene07181	PPI
MS.gene70642	MS.gene07181	PPI
MS.gene005628	MS.gene07181	PPI
MS.gene70641	MS.gene07181	PPI
MS.gene07181	MS.gene005628	PPI
MS.gene005632	MS.gene07181	PPI
MS.gene07179	MS.gene07181	PPI
MS.gene23009	MS.gene07181	PPI
MS.gene041519	MS.gene07181	PPI
MS.gene005631	MS.gene07181	PPI
MS.gene035291	MS.gene07181	PPI
MS.gene23005	MS.gene07181	PPI
MS.gene29370	MS.gene07181	PPI
MS.gene58937	MS.gene07181	PPI
MS.gene29371	MS.gene07181	PPI
MS.gene03023	MS.gene07181	PPI
MS.gene035294	MS.gene07181	PPI
MS.gene58935	MS.gene07181	PPI
MS.gene07180	MS.gene07181	PPI
MS.gene050229	MS.gene07181	PPI
MS.gene036528	MS.gene07181	PPI
MS.gene03026	MS.gene07181	PPI
MS.gene25978	MS.gene07181	PPI
MS.gene006983	MS.gene07181	PPI
MS.gene005630	MS.gene07181	PPI
MS.gene006980	MS.gene07181	PPI
MS.gene041518	MS.gene07181	PPI
MS.gene07181	MS.gene005631	PPI
MS.gene20704	MS.gene07181	PPI
MS.gene07182	MS.gene07181	PPI
MS.gene52509	MS.gene07181	PPI
MS.gene035290	MS.gene07181	PPI
MS.gene87167	MS.gene07181	PPI
MS.gene20705	MS.gene07181	PPI
MS.gene022311	MS.gene07181	PPI
MS.gene036527	MS.gene07181	PPI
MS.gene59643	MS.gene07181	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07181.t1	MTR_1g088910	100.000	102	0	0	1	102	1	102	6.54e-74	214
MS.gene07181.t1	MTR_7g108220	89.320	103	10	1	1	102	1	103	1.42e-63	187
MS.gene07181.t1	MTR_7g108210	88.350	103	11	1	1	102	1	103	6.74e-63	186
MS.gene07181.t1	MTR_2g019950	63.725	102	37	0	1	102	1	102	8.83e-45	140
MS.gene07181.t1	MTR_2g019900	58.416	101	42	0	1	101	1	101	4.46e-39	126
MS.gene07181.t1	MTR_7g108200	57.447	94	40	0	1	94	1	94	1.79e-37	122
MS.gene07181.t1	MTR_1g088920	54.082	98	45	0	1	98	1	98	1.74e-36	119
MS.gene07181.t1	MTR_1g088925	52.041	98	47	0	1	98	1	98	3.60e-36	118
MS.gene07181.t1	MTR_4g119050	53.398	103	47	1	1	102	1	103	3.11e-33	111
MS.gene07181.t1	MTR_4g119030	53.398	103	47	1	1	102	1	103	3.11e-33	111
MS.gene07181.t1	MTR_4g119040	53.398	103	47	1	1	102	1	103	3.11e-33	111
MS.gene07181.t1	MTR_7g026770	51.456	103	49	1	1	102	22	124	5.95e-32	113
MS.gene07181.t1	MTR_7g108250	49.020	102	52	0	1	102	1	102	2.62e-30	103
MS.gene07181.t1	MTR_1g088905	49.020	102	52	0	1	102	1	102	5.10e-30	103
MS.gene07181.t1	MTR_7g022550	47.573	103	53	1	1	102	30	132	7.81e-30	103
MS.gene07181.t1	MTR_7g022690	47.573	103	53	1	1	102	30	132	9.00e-30	103
MS.gene07181.t1	MTR_7g022710	47.573	103	53	1	1	102	30	132	2.80e-29	102
MS.gene07181.t1	MTR_7g108260	45.098	102	56	0	1	102	1	102	7.09e-29	100
MS.gene07181.t1	MTR_1g088895	48.485	99	51	0	4	102	4	102	2.74e-25	91.7
MS.gene07181.t1	MTR_2g048970	39.048	105	60	1	2	102	29	133	5.99e-18	73.6
MS.gene07181.t1	MTR_3g077570	37.000	100	63	0	1	100	18	117	8.17e-17	70.1
MS.gene07181.t1	MTR_7g035245	35.000	100	65	0	1	100	3	102	6.95e-16	67.4
MS.gene07181.t1	MTR_1g069255	34.343	99	64	1	2	99	84	182	1.71e-15	68.6
MS.gene07181.t1	MTR_2g090755	32.673	101	61	2	6	102	43	140	2.04e-15	67.0
MS.gene07181.t1	MTR_3g077560	34.000	100	66	0	1	100	21	120	1.46e-14	64.7
MS.gene07181.t1	MTR_3g104510	35.000	100	59	2	3	101	23	117	2.51e-14	63.9
MS.gene07181.t1	MTR_4g079110	33.333	99	63	2	6	102	58	155	5.98e-14	63.5
MS.gene07181.t1	MTR_1g015890	37.255	102	60	2	3	101	48	148	1.03e-13	63.2
MS.gene07181.t1	MTR_2g014760	30.000	100	70	0	3	102	28	127	1.16e-13	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07181.t1	AT3G62950	74.757	103	25	1	1	102	1	103	1.02e-55	168
MS.gene07181.t1	AT2G47870	74.757	103	25	1	1	102	1	103	2.51e-53	162
MS.gene07181.t1	AT3G21460	55.882	102	45	0	1	102	1	102	1.21e-39	127
MS.gene07181.t1	AT2G47880	49.020	102	52	0	1	102	1	102	1.67e-34	114
MS.gene07181.t1	AT4G15690	48.039	102	53	0	1	102	1	102	2.90e-33	111
MS.gene07181.t1	AT5G18600	50.495	101	50	0	1	101	1	101	3.10e-33	111
MS.gene07181.t1	AT4G15670	47.059	102	54	0	1	102	1	102	5.24e-33	110
MS.gene07181.t1	AT4G15700	48.039	102	53	0	1	102	1	102	5.66e-33	110
MS.gene07181.t1	AT4G15680	47.059	102	54	0	1	102	1	102	6.46e-33	110
MS.gene07181.t1	AT3G62960	52.128	94	45	0	1	94	1	94	1.18e-32	110
MS.gene07181.t1	AT2G30540	48.485	99	51	0	1	99	1	99	1.08e-31	107
MS.gene07181.t1	AT4G15660	46.078	102	55	0	1	102	1	102	1.20e-31	107
MS.gene07181.t1	AT1G06830	52.222	90	43	0	1	90	1	90	3.79e-31	105
MS.gene07181.t1	AT3G62930	49.505	101	51	0	1	101	1	101	1.43e-29	102
MS.gene07181.t1	AT5G14070	46.364	110	51	2	1	102	31	140	2.61e-29	102
MS.gene07181.t1	AT1G03020	47.059	102	54	0	1	102	1	102	4.79e-28	98.2
MS.gene07181.t1	AT3G02000	47.170	106	50	1	3	102	31	136	1.04e-25	93.6
MS.gene07181.t1	AT5G40370	34.000	100	66	0	1	100	3	102	9.75e-16	67.4
MS.gene07181.t1	AT1G28480	30.476	105	69	2	2	102	33	137	4.02e-15	66.2
MS.gene07181.t1	AT4G28730	32.323	99	66	1	2	99	71	169	2.29e-14	65.1
MS.gene07181.t1	AT5G63030	34.000	100	66	0	1	100	19	118	5.89e-14	63.2
MS.gene07181.t1	AT4G33040	33.333	105	64	2	3	102	41	144	2.40e-13	62.0
MS.gene07181.t1	AT5G20500	37.634	93	58	0	2	94	33	125	5.12e-13	60.8
MS.gene07181.t1	AT2G20270	31.313	99	67	1	2	99	76	174	5.61e-12	58.9
MS.gene07181.t1	AT5G40370	36.047	86	55	0	15	100	42	127	3.33e-11	56.2
MS.gene07181.t1	AT5G11930	33.000	100	57	3	3	101	57	147	3.40e-11	56.6

Find 28 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATGGCTGCTAGGGCCATT+TGG	0.373171	1.4:+70656169	MS.gene07181:CDS
TTCATTGGTGGAAAATTTGT+AGG	0.394277	1.4:+70656093	MS.gene07181:CDS
TCAGTGCCAGCAGTGTTCAT+TGG	0.405054	1.4:+70656078	MS.gene07181:CDS
GTGATATCTCTTCATGTTGA+TGG	0.409792	1.4:+70656129	MS.gene07181:CDS
TGAACCCTATGGTAGGGAAA+TGG	0.445205	1.4:+70656023	MS.gene07181:CDS
AATGGATAGAGTGAAGGATT+TGG	0.446072	1.4:+70655888	None:intergenic
ATGAACACTGCTGGCACTGA+AGG	0.449282	1.4:-70656075	None:intergenic
GAAAGGGCTTTGAGGAGTCT+TGG	0.459714	1.4:+70656045	MS.gene07181:CDS
GGGAAATGGAAAGGGCTTTG+AGG	0.466190	1.4:+70656037	MS.gene07181:CDS
CTTTCCATTTCCCTACCATA+GGG	0.475427	1.4:-70656027	None:intergenic
CCTTTCCATTTCCCTACCAT+AGG	0.487337	1.4:-70656028	None:intergenic
TTGTCAAGCTCATGCACAGC+AGG	0.496748	1.4:-70656000	None:intergenic
ACAAATGCTCATGGCTGCTA+GGG	0.507037	1.4:+70656161	MS.gene07181:CDS
AACAAATGCTCATGGCTGCT+AGG	0.510969	1.4:+70656160	MS.gene07181:CDS
TTTCCACCAATGAACACTGC+TGG	0.522815	1.4:-70656084	None:intergenic
GTGCCAGCAGTGTTCATTGG+TGG	0.525941	1.4:+70656081	MS.gene07181:CDS
TTAAGTAATGGATAGAGTGA+AGG	0.538058	1.4:+70655882	None:intergenic
CTATGGTAGGGAAATGGAAA+GGG	0.543036	1.4:+70656029	MS.gene07181:CDS
CTCACTCAAACAAATGCTCA+TGG	0.566311	1.4:+70656152	MS.gene07181:CDS
TCATTGGTGGAAAATTTGTA+GGG	0.566956	1.4:+70656094	MS.gene07181:CDS
ACACAATTGTTCTATGAGCT+AGG	0.582609	1.4:+70655970	MS.gene07181:CDS
AAAATTTGTAGGGTCATCAA+AGG	0.583330	1.4:+70656104	MS.gene07181:CDS
TTGACAATGAACCCTATGGT+AGG	0.584348	1.4:+70656016	MS.gene07181:CDS
GAGCTTGACAATGAACCCTA+TGG	0.586058	1.4:+70656012	MS.gene07181:CDS
TGACAATGAACCCTATGGTA+GGG	0.596777	1.4:+70656017	MS.gene07181:CDS
TAGTTAGTACTAGAACCAAA+TGG	0.602036	1.4:-70656184	None:intergenic
TGAACACTGCTGGCACTGAA+GGG	0.607813	1.4:-70656074	None:intergenic
CCTATGGTAGGGAAATGGAA+AGG	0.608746	1.4:+70656028	MS.gene07181:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAATTTGTAGGGTCATCAA+AGG	+	chr1.4:70656104-70656123	MS.gene07181:CDS	30.0%
	TCATTGGTGGAAAATTTGTA+GGG	+	chr1.4:70656094-70656113	MS.gene07181:CDS	30.0%
	TTCATTGGTGGAAAATTTGT+AGG	+	chr1.4:70656093-70656112	MS.gene07181:CDS	30.0%
	ACACAATTGTTCTATGAGCT+AGG	+	chr1.4:70655970-70655989	MS.gene07181:CDS	35.0%
	GTGATATCTCTTCATGTTGA+TGG	+	chr1.4:70656129-70656148	MS.gene07181:CDS	35.0%
	CTATGGTAGGGAAATGGAAA+GGG	+	chr1.4:70656029-70656048	MS.gene07181:CDS	40.0%
	CTCACTCAAACAAATGCTCA+TGG	+	chr1.4:70656152-70656171	MS.gene07181:CDS	40.0%
	CTTTCCATTTCCCTACCATA+GGG	-	chr1.4:70656030-70656049	None:intergenic	40.0%
	TGACAATGAACCCTATGGTA+GGG	+	chr1.4:70656017-70656036	MS.gene07181:CDS	40.0%
	TTGACAATGAACCCTATGGT+AGG	+	chr1.4:70656016-70656035	MS.gene07181:CDS	40.0%
	AACAAATGCTCATGGCTGCT+AGG	+	chr1.4:70656160-70656179	MS.gene07181:CDS	45.0%
	ACAAATGCTCATGGCTGCTA+GGG	+	chr1.4:70656161-70656180	MS.gene07181:CDS	45.0%
	CCTATGGTAGGGAAATGGAA+AGG	+	chr1.4:70656028-70656047	MS.gene07181:CDS	45.0%
	CCTTTCCATTTCCCTACCAT+AGG	-	chr1.4:70656031-70656050	None:intergenic	45.0%
	GAGCTTGACAATGAACCCTA+TGG	+	chr1.4:70656012-70656031	MS.gene07181:CDS	45.0%
	TGAACCCTATGGTAGGGAAA+TGG	+	chr1.4:70656023-70656042	MS.gene07181:CDS	45.0%
	TTTCCACCAATGAACACTGC+TGG	-	chr1.4:70656087-70656106	None:intergenic	45.0%
	GGGAAATGGAAAGGGCTTTG+AGG	+	chr1.4:70656037-70656056	MS.gene07181:CDS	50.0%
	TCAGTGCCAGCAGTGTTCAT+TGG	+	chr1.4:70656078-70656097	MS.gene07181:CDS	50.0%
	TTGTCAAGCTCATGCACAGC+AGG	-	chr1.4:70656003-70656022	None:intergenic	50.0%
!	ATGAACACTGCTGGCACTGA+AGG	-	chr1.4:70656078-70656097	None:intergenic	50.0%
!	TGAACACTGCTGGCACTGAA+GGG	-	chr1.4:70656077-70656096	None:intergenic	50.0%
!!	GAAAGGGCTTTGAGGAGTCT+TGG	+	chr1.4:70656045-70656064	MS.gene07181:CDS	50.0%
	GTGCCAGCAGTGTTCATTGG+TGG	+	chr1.4:70656081-70656100	MS.gene07181:CDS	55.0%
	TCATGGCTGCTAGGGCCATT+TGG	+	chr1.4:70656169-70656188	MS.gene07181:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	70655889	70656197	70655889	ID=MS.gene07181
chr1.4	mRNA	70655889	70656197	70655889	ID=MS.gene07181.t1;Parent=MS.gene07181
chr1.4	exon	70655889	70656197	70655889	ID=MS.gene07181.t1.exon1;Parent=MS.gene07181.t1
chr1.4	CDS	70655889	70656197	70655889	ID=cds.MS.gene07181.t1;Parent=MS.gene07181.t1

Gene Sequence	>MS.gene07181 ATGGATAGAGTGAAGGATTTGGCATCAAAGAAAGCTGCAGTTATATTCACAAAGAGTTCATGCTACATGTGTCACAGCATAACACAATTGTTCTATGAGCTAGGAGCAAGTCCTGCTGTGCATGAGCTTGACAATGAACCCTATGGTAGGGAAATGGAAAGGGCTTTGAGGAGTCTTGGATGTAACCCTTCAGTGCCAGCAGTGTTCATTGGTGGAAAATTTGTAGGGTCATCAAAGGATGTGATATCTCTTCATGTTGATGGCTCACTCAAACAAATGCTCATGGCTGCTAGGGCCATTTGGTTCTAG
Protein sequence	>MS.gene07181.t1 MDRVKDLASKKAAVIFTKSSCYMCHSITQLFYELGASPAVHELDNEPYGREMERALRSLGCNPSVPAVFIGGKFVGSSKDVISLHVDGSLKQMLMAARAIWF