AlfalfaGEDB | MS.gene07182

M. sativa cultivar XinJiangDaYe / MS.gene07182

KEGG: K03676
GO: GO:0003674 GO:0003824 GO:0008150 GO:0009055 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0019725 GO:0042592 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008
PFAM: PF00462.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07182.t1	XP_013469212.1	95.1	102	5	0	1	102	1	102	1.80E-46	194.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07182.t1	Q9LYC8	62.7	102	38	0	1	102	1	102	3.1e-34	145.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07182.t1	A0A072VYV0	95.1	102	5	0	1	102	1	102	1.3e-46	194.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene25976	MS.gene07182	PPI
MS.gene07182	MS.gene20705	PPI
MS.gene07182	MS.gene23009	PPI
MS.gene035290	MS.gene07182	PPI
MS.gene041521	MS.gene07182	PPI
MS.gene87167	MS.gene07182	PPI
MS.gene23007	MS.gene07182	PPI
MS.gene07182	MS.gene005628	PPI
MS.gene07182	MS.gene005630	PPI
MS.gene07182	MS.gene035294	PPI
MS.gene07182	MS.gene59643	PPI
MS.gene70642	MS.gene07182	PPI
MS.gene07182	MS.gene035291	PPI
MS.gene20703	MS.gene07182	PPI
MS.gene07182	MS.gene20704	PPI
MS.gene07182	MS.gene005629	PPI
MS.gene07179	MS.gene07182	PPI
MS.gene07182	MS.gene58937	PPI
MS.gene07182	MS.gene005631	PPI
MS.gene23005	MS.gene07182	PPI
MS.gene036524	MS.gene07182	PPI
MS.gene07182	MS.gene041518	PPI
MS.gene07182	MS.gene70641	PPI
MS.gene03023	MS.gene07182	PPI
MS.gene022312	MS.gene07182	PPI
MS.gene07182	MS.gene006984	PPI
MS.gene005632	MS.gene07182	PPI
MS.gene58935	MS.gene07182	PPI
MS.gene07180	MS.gene07182	PPI
MS.gene07182	MS.gene58938	PPI
MS.gene041519	MS.gene07182	PPI
MS.gene07182	MS.gene022311	PPI
MS.gene036528	MS.gene07182	PPI
MS.gene29371	MS.gene07182	PPI
MS.gene25978	MS.gene07182	PPI
MS.gene006980	MS.gene07182	PPI
MS.gene07182	MS.gene20701	PPI
MS.gene07182	MS.gene03026	PPI
MS.gene07182	MS.gene036527	PPI
MS.gene07182	MS.gene29370	PPI
MS.gene07182	MS.gene006982	PPI
MS.gene005633	MS.gene07182	PPI
MS.gene29979	MS.gene07182	PPI
MS.gene07182	MS.gene050229	PPI
MS.gene07182	MS.gene52509	PPI
MS.gene20702	MS.gene07182	PPI
MS.gene07182	MS.gene07181	PPI
MS.gene07182	MS.gene23008	PPI
MS.gene006983	MS.gene07182	PPI
MS.gene07182	MS.gene03024	PPI
MS.gene07182	MS.gene03022	PPI
MS.gene07183	MS.gene07182	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07182.t1	MTR_1g088905	95.098	102	5	0	1	102	1	102	1.62e-68	200
MS.gene07182.t1	MTR_7g108250	77.451	102	23	0	1	102	1	102	2.52e-58	174
MS.gene07182.t1	MTR_4g119050	58.252	103	42	1	1	102	1	103	5.49e-42	133
MS.gene07182.t1	MTR_4g119030	58.252	103	42	1	1	102	1	103	5.49e-42	133
MS.gene07182.t1	MTR_4g119040	58.252	103	42	1	1	102	1	103	5.49e-42	133
MS.gene07182.t1	MTR_2g019900	54.902	102	46	0	1	102	1	102	1.68e-41	132
MS.gene07182.t1	MTR_1g088895	53.922	102	47	0	1	102	1	102	4.67e-39	126
MS.gene07182.t1	MTR_7g108260	55.446	101	45	0	1	101	1	101	3.89e-38	124
MS.gene07182.t1	MTR_2g019950	53.922	102	47	0	1	102	1	102	4.78e-36	118
MS.gene07182.t1	MTR_1g088910	50.000	102	51	0	1	102	1	102	2.45e-32	108
MS.gene07182.t1	MTR_7g108220	48.544	103	52	1	1	102	1	103	1.44e-30	104
MS.gene07182.t1	MTR_7g108210	47.573	103	53	1	1	102	1	103	7.87e-30	102
MS.gene07182.t1	MTR_7g022690	43.689	103	57	1	1	102	30	132	2.15e-27	97.4
MS.gene07182.t1	MTR_7g026770	48.544	103	52	1	1	102	22	124	4.78e-27	100
MS.gene07182.t1	MTR_7g022710	41.748	103	59	1	1	102	30	132	6.18e-27	96.3
MS.gene07182.t1	MTR_1g088920	41.489	94	55	0	1	94	1	94	8.68e-27	95.1
MS.gene07182.t1	MTR_7g108200	43.617	94	53	0	1	94	1	94	1.12e-26	94.7
MS.gene07182.t1	MTR_1g088925	39.362	94	57	0	1	94	1	94	1.32e-26	94.4
MS.gene07182.t1	MTR_7g022550	40.777	103	60	1	1	102	30	132	4.14e-25	91.7
MS.gene07182.t1	MTR_2g090755	38.462	104	59	2	2	102	39	140	4.30e-21	81.6
MS.gene07182.t1	MTR_2g048970	40.000	105	59	1	2	102	29	133	2.00e-20	79.7
MS.gene07182.t1	MTR_2g014760	35.354	99	64	0	4	102	29	127	8.88e-20	78.2
MS.gene07182.t1	MTR_3g077570	38.000	100	62	0	1	100	18	117	1.20e-19	77.4
MS.gene07182.t1	MTR_4g079110	37.255	102	61	2	3	102	55	155	2.60e-19	77.4
MS.gene07182.t1	MTR_3g077560	36.000	100	64	0	1	100	21	120	2.25e-18	74.3
MS.gene07182.t1	MTR_7g035245	43.158	95	54	0	6	100	8	102	3.43e-18	73.6
MS.gene07182.t1	MTR_1g015890	36.634	101	62	1	4	102	49	149	2.30e-17	72.4
MS.gene07182.t1	MTR_1g069255	37.374	99	61	1	2	99	84	182	5.83e-16	69.7
MS.gene07182.t1	MTR_3g104510	35.354	99	58	3	4	101	24	117	1.43e-14	64.3
MS.gene07182.t1	MTR_5g077550	38.462	104	60	2	2	102	21	123	1.91e-14	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07182.t1	AT3G62930	62.745	102	38	0	1	102	1	102	3.02e-48	149
MS.gene07182.t1	AT1G03020	58.824	102	42	0	1	102	1	102	2.17e-46	144
MS.gene07182.t1	AT5G18600	59.804	102	41	0	1	102	1	102	4.24e-46	144
MS.gene07182.t1	AT4G15680	57.843	102	43	0	1	102	1	102	1.27e-43	137
MS.gene07182.t1	AT4G15690	57.843	102	43	0	1	102	1	102	4.63e-43	136
MS.gene07182.t1	AT4G15700	56.863	102	44	0	1	102	1	102	5.83e-43	135
MS.gene07182.t1	AT4G15670	53.922	102	47	0	1	102	1	102	2.37e-41	132
MS.gene07182.t1	AT4G15660	54.902	102	46	0	1	102	1	102	6.94e-41	130
MS.gene07182.t1	AT3G21460	48.039	102	53	0	1	102	1	102	2.52e-33	111
MS.gene07182.t1	AT3G62950	45.631	103	55	1	1	102	1	103	2.87e-29	101
MS.gene07182.t1	AT1G06830	43.617	94	53	0	1	94	1	94	4.55e-28	98.2
MS.gene07182.t1	AT2G47870	40.777	103	60	1	1	102	1	103	4.87e-28	98.2
MS.gene07182.t1	AT2G30540	43.617	94	53	0	1	94	1	94	9.13e-28	97.4
MS.gene07182.t1	AT2G47880	41.489	94	55	0	1	94	1	94	1.65e-26	94.4
MS.gene07182.t1	AT5G14070	42.991	107	53	2	4	102	34	140	6.23e-26	94.0
MS.gene07182.t1	AT3G62960	40.426	94	56	0	1	94	1	94	2.54e-25	91.3
MS.gene07182.t1	AT3G02000	43.810	105	53	1	4	102	32	136	2.59e-23	87.4
MS.gene07182.t1	AT5G63030	43.158	95	54	0	6	100	24	118	3.63e-20	79.0
MS.gene07182.t1	AT4G33040	37.864	103	60	1	4	102	42	144	1.81e-18	75.1
MS.gene07182.t1	AT5G40370	40.000	100	60	0	1	100	3	102	4.89e-18	73.2
MS.gene07182.t1	AT1G28480	31.429	105	68	2	2	102	33	137	5.99e-17	71.2
MS.gene07182.t1	AT5G11930	34.343	99	55	3	4	101	58	147	1.62e-16	70.1
MS.gene07182.t1	AT2G20270	35.354	99	63	1	2	99	76	174	1.81e-16	70.9
MS.gene07182.t1	AT1G03850	32.292	96	61	1	7	102	59	150	3.98e-15	66.6
MS.gene07182.t1	AT5G40370	40.698	86	51	0	15	100	42	127	1.06e-13	62.8
MS.gene07182.t1	AT4G28730	33.333	99	65	1	2	99	71	169	1.56e-12	60.5
MS.gene07182.t1	AT5G20500	32.990	97	65	0	2	98	33	129	4.32e-12	58.5

Find 26 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTGTACCAGTTGTGTTTAT+TGG	0.272819	1.4:-70630139	MS.gene07182:CDS
TCATCAAGCTCATAAACAAT+TGG	0.354952	1.4:+70630217	None:intergenic
TCATGAATGCCGGAGCTATA+TGG	0.387749	1.4:-70630048	MS.gene07182:CDS
TAGAGCCACCAATGAATTGT+TGG	0.395335	1.4:+70630116	None:intergenic
GAGCTTGATGAGATAACAAA+TGG	0.402669	1.4:-70630205	MS.gene07182:CDS
CATCAAGCTCATAAACAATT+GGG	0.430944	1.4:+70630218	None:intergenic
GAATCATGACCCTTCATCTT+CGG	0.437884	1.4:-70630090	MS.gene07182:CDS
TGTTGGCCAATAAACACAAC+TGG	0.465473	1.4:+70630133	None:intergenic
TTTATTGGCCAACAATTCAT+TGG	0.465893	1.4:-70630124	MS.gene07182:CDS
ATGACATTGATACGAAGCTT+TGG	0.484478	1.4:-70630247	MS.gene07182:CDS
CTAGCTCATTCCGAAGATGA+AGG	0.506749	1.4:+70630080	None:intergenic
ATTGGCCAACAATTCATTGG+TGG	0.532087	1.4:-70630121	MS.gene07182:CDS
AAATTAAATCCATATAGCTC+CGG	0.539907	1.4:+70630039	None:intergenic
GAAAGAGCACTGATTCAACT+AGG	0.544425	1.4:-70630172	MS.gene07182:CDS
ACACAACTGGTACACTTGGT+TGG	0.550739	1.4:+70630146	None:intergenic
GTACCTATGCTCATGAATGC+CGG	0.552236	1.4:-70630058	MS.gene07182:CDS
CTCTTGCTGAATATCACCAC+AGG	0.554486	1.4:+70630295	None:intergenic
ATAAACACAACTGGTACACT+TGG	0.565056	1.4:+70630142	None:intergenic
GCTTCGTATCAATGTCATGA+TGG	0.581724	1.4:+70630252	None:intergenic
AGCTTGATGAGATAACAAAT+GGG	0.597901	1.4:-70630204	MS.gene07182:CDS
TAATCATGGACATGATTACA+AGG	0.612323	1.4:-70630333	None:intergenic
TAGCTCATTCCGAAGATGAA+GGG	0.619643	1.4:+70630081	None:intergenic
AAAGAGCACTGATTCAACTA+GGG	0.626942	1.4:-70630171	MS.gene07182:CDS
GTATCAATGTCATGATGGAG+TGG	0.644776	1.4:+70630257	None:intergenic
GCTCCGGCATTCATGAGCAT+AGG	0.659701	1.4:+70630055	None:intergenic
GATAGTGAGTGATAAACCTG+TGG	0.751670	1.4:-70630311	MS.gene07182:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGCTTGATGAGATAACAAAT+GGG	-	chr1.4:70630166-70630185	MS.gene07182:CDS	30.0%
	CATCAAGCTCATAAACAATT+GGG	+	chr1.4:70630155-70630174	None:intergenic	30.0%
	TCATCAAGCTCATAAACAAT+TGG	+	chr1.4:70630156-70630175	None:intergenic	30.0%
	TTTATTGGCCAACAATTCAT+TGG	-	chr1.4:70630246-70630265	MS.gene07182:CDS	30.0%
	AGTGTACCAGTTGTGTTTAT+TGG	-	chr1.4:70630231-70630250	MS.gene07182:CDS	35.0%
	ATAAACACAACTGGTACACT+TGG	+	chr1.4:70630231-70630250	None:intergenic	35.0%
	ATGACATTGATACGAAGCTT+TGG	-	chr1.4:70630123-70630142	MS.gene07182:CDS	35.0%
	GAGCTTGATGAGATAACAAA+TGG	-	chr1.4:70630165-70630184	MS.gene07182:CDS	35.0%
!!	AAAGAGCACTGATTCAACTA+GGG	-	chr1.4:70630199-70630218	MS.gene07182:CDS	35.0%
	ATTGGCCAACAATTCATTGG+TGG	-	chr1.4:70630249-70630268	MS.gene07182:CDS	40.0%
	GAATCATGACCCTTCATCTT+CGG	-	chr1.4:70630280-70630299	MS.gene07182:CDS	40.0%
	GATAGTGAGTGATAAACCTG+TGG	-	chr1.4:70630059-70630078	MS.gene07182:CDS	40.0%
	GCTTCGTATCAATGTCATGA+TGG	+	chr1.4:70630121-70630140	None:intergenic	40.0%
	TAGAGCCACCAATGAATTGT+TGG	+	chr1.4:70630257-70630276	None:intergenic	40.0%
	TAGCTCATTCCGAAGATGAA+GGG	+	chr1.4:70630292-70630311	None:intergenic	40.0%
	TGTTGGCCAATAAACACAAC+TGG	+	chr1.4:70630240-70630259	None:intergenic	40.0%
!	GTATCAATGTCATGATGGAG+TGG	+	chr1.4:70630116-70630135	None:intergenic	40.0%
!!	GAAAGAGCACTGATTCAACT+AGG	-	chr1.4:70630198-70630217	MS.gene07182:CDS	40.0%
	ACACAACTGGTACACTTGGT+TGG	+	chr1.4:70630227-70630246	None:intergenic	45.0%
	CTAGCTCATTCCGAAGATGA+AGG	+	chr1.4:70630293-70630312	None:intergenic	45.0%
	CTCTTGCTGAATATCACCAC+AGG	+	chr1.4:70630078-70630097	None:intergenic	45.0%
	GTACCTATGCTCATGAATGC+CGG	-	chr1.4:70630312-70630331	MS.gene07182:CDS	45.0%
	TCATGAATGCCGGAGCTATA+TGG	-	chr1.4:70630322-70630341	MS.gene07182:CDS	45.0%
	GCTCCGGCATTCATGAGCAT+AGG	+	chr1.4:70630318-70630337	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	70630042	70630350	70630042	ID=MS.gene07182
chr1.4	mRNA	70630042	70630350	70630042	ID=MS.gene07182.t1;Parent=MS.gene07182
chr1.4	exon	70630042	70630350	70630042	ID=MS.gene07182.t1.exon1;Parent=MS.gene07182.t1
chr1.4	CDS	70630042	70630350	70630042	ID=cds.MS.gene07182.t1;Parent=MS.gene07182.t1

Gene Sequence	>MS.gene07182 ATGGACATGATTACAAGGATAGTGAGTGATAAACCTGTGGTGATATTCAGCAAGAGCACATGTTGTTTGAGCCACTCCATCATGACATTGATACGAAGCTTTGGTGCAAACCCAATTGTTTATGAGCTTGATGAGATAACAAATGGGTCACAGATAGAAAGAGCACTGATTCAACTAGGGTGCCAACCAAGTGTACCAGTTGTGTTTATTGGCCAACAATTCATTGGTGGCTCTAAAAGAATCATGACCCTTCATCTTCGGAATGAGCTAGTACCTATGCTCATGAATGCCGGAGCTATATGGATTTAA
Protein sequence	>MS.gene07182.t1 MDMITRIVSDKPVVIFSKSTCCLSHSIMTLIRSFGANPIVYELDEITNGSQIERALIQLGCQPSVPVVFIGQQFIGGSKRIMTLHLRNELVPMLMNAGAIWI