AlfalfaGEDB | MS.gene050229

M. sativa cultivar XinJiangDaYe / MS.gene050229

KEGG: K03676
GO: GO:0003674 GO:0003824 GO:0008150 GO:0009055 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0019725 GO:0042592 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008
PFAM: PF00462.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050229.t1	XP_013469212.1	95.1	102	5	0	3	104	1	102	2.40E-46	194.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050229.t1	Q9LYC8	62.7	102	38	0	3	104	1	102	4.1e-34	145.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050229.t1	A0A072VYV0	95.1	102	5	0	3	104	1	102	1.8e-46	194.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene006984	MS.gene050229	PPI
MS.gene20702	MS.gene050229	PPI
MS.gene050229	MS.gene03024	PPI
MS.gene006983	MS.gene050229	PPI
MS.gene050229	MS.gene03022	PPI
MS.gene07183	MS.gene050229	PPI
MS.gene035290	MS.gene050229	PPI
MS.gene87167	MS.gene050229	PPI
MS.gene036527	MS.gene050229	PPI
MS.gene23007	MS.gene050229	PPI
MS.gene050229	MS.gene005630	PPI
MS.gene005629	MS.gene050229	PPI
MS.gene23008	MS.gene050229	PPI
MS.gene03022	MS.gene050229	PPI
MS.gene70642	MS.gene050229	PPI
MS.gene20703	MS.gene050229	PPI
MS.gene07179	MS.gene050229	PPI
MS.gene23009	MS.gene050229	PPI
MS.gene25976	MS.gene050229	PPI
MS.gene041521	MS.gene050229	PPI
MS.gene050229	MS.gene005631	PPI
MS.gene050229	MS.gene022311	PPI
MS.gene58938	MS.gene050229	PPI
MS.gene23005	MS.gene050229	PPI
MS.gene036524	MS.gene050229	PPI
MS.gene03023	MS.gene050229	PPI
MS.gene022312	MS.gene050229	PPI
MS.gene050229	MS.gene006984	PPI
MS.gene035294	MS.gene050229	PPI
MS.gene050229	MS.gene20701	PPI
MS.gene70641	MS.gene050229	PPI
MS.gene005632	MS.gene050229	PPI
MS.gene58935	MS.gene050229	PPI
MS.gene07180	MS.gene050229	PPI
MS.gene041519	MS.gene050229	PPI
MS.gene035291	MS.gene050229	PPI
MS.gene036528	MS.gene050229	PPI
MS.gene29370	MS.gene050229	PPI
MS.gene58937	MS.gene050229	PPI
MS.gene050229	MS.gene07181	PPI
MS.gene29371	MS.gene050229	PPI
MS.gene03026	MS.gene050229	PPI
MS.gene25978	MS.gene050229	PPI
MS.gene005630	MS.gene050229	PPI
MS.gene006980	MS.gene050229	PPI
MS.gene041518	MS.gene050229	PPI
MS.gene20704	MS.gene050229	PPI
MS.gene07182	MS.gene050229	PPI
MS.gene52509	MS.gene050229	PPI
MS.gene20705	MS.gene050229	PPI
MS.gene050229	MS.gene006982	PPI
MS.gene050229	MS.gene005628	PPI
MS.gene59643	MS.gene050229	PPI
MS.gene03024	MS.gene050229	PPI
MS.gene005633	MS.gene050229	PPI
MS.gene29979	MS.gene050229	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050229.t1	MTR_1g088905	95.098	102	5	0	3	104	1	102	1.29e-68	201
MS.gene050229.t1	MTR_7g108250	77.451	102	23	0	3	104	1	102	2.19e-58	175
MS.gene050229.t1	MTR_4g119050	58.252	103	42	1	3	104	1	103	5.04e-42	133
MS.gene050229.t1	MTR_4g119030	58.252	103	42	1	3	104	1	103	5.04e-42	133
MS.gene050229.t1	MTR_4g119040	58.252	103	42	1	3	104	1	103	5.04e-42	133
MS.gene050229.t1	MTR_2g019900	54.902	102	46	0	3	104	1	102	1.42e-41	132
MS.gene050229.t1	MTR_1g088895	53.922	102	47	0	3	104	1	102	4.48e-39	126
MS.gene050229.t1	MTR_7g108260	55.446	101	45	0	3	103	1	101	3.85e-38	124
MS.gene050229.t1	MTR_2g019950	53.922	102	47	0	3	104	1	102	4.59e-36	118
MS.gene050229.t1	MTR_1g088910	50.000	102	51	0	3	104	1	102	2.01e-32	109
MS.gene050229.t1	MTR_7g108220	48.544	103	52	1	3	104	1	103	1.32e-30	104
MS.gene050229.t1	MTR_7g108210	47.573	103	53	1	3	104	1	103	7.37e-30	102
MS.gene050229.t1	MTR_7g022690	43.689	103	57	1	3	104	30	132	2.34e-27	97.4
MS.gene050229.t1	MTR_7g026770	48.544	103	52	1	3	104	22	124	4.55e-27	100
MS.gene050229.t1	MTR_7g022710	41.748	103	59	1	3	104	30	132	6.74e-27	96.3
MS.gene050229.t1	MTR_1g088920	41.489	94	55	0	3	96	1	94	9.67e-27	95.1
MS.gene050229.t1	MTR_7g108200	43.617	94	53	0	3	96	1	94	1.12e-26	94.7
MS.gene050229.t1	MTR_1g088925	39.362	94	57	0	3	96	1	94	1.50e-26	94.4
MS.gene050229.t1	MTR_7g022550	40.777	103	60	1	3	104	30	132	4.98e-25	91.7
MS.gene050229.t1	MTR_2g090755	38.462	104	59	2	4	104	39	140	5.51e-21	81.6
MS.gene050229.t1	MTR_2g048970	40.000	105	59	1	4	104	29	133	2.06e-20	79.7
MS.gene050229.t1	MTR_2g014760	35.354	99	64	0	6	104	29	127	9.44e-20	78.2
MS.gene050229.t1	MTR_3g077570	38.000	100	62	0	3	102	18	117	1.38e-19	77.4
MS.gene050229.t1	MTR_4g079110	37.255	102	61	2	5	104	55	155	3.29e-19	77.4
MS.gene050229.t1	MTR_3g077560	36.000	100	64	0	3	102	21	120	2.75e-18	74.3
MS.gene050229.t1	MTR_7g035245	43.158	95	54	0	8	102	8	102	3.93e-18	73.2
MS.gene050229.t1	MTR_1g015890	36.634	101	62	1	6	104	49	149	2.49e-17	72.4
MS.gene050229.t1	MTR_1g069255	36.634	101	63	1	2	101	82	182	5.99e-16	69.7
MS.gene050229.t1	MTR_3g104510	35.354	99	58	3	6	103	24	117	1.76e-14	64.3
MS.gene050229.t1	MTR_5g077550	38.462	104	60	2	4	104	21	123	2.07e-14	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050229.t1	AT3G62930	62.745	102	38	0	3	104	1	102	2.66e-48	149
MS.gene050229.t1	AT1G03020	58.824	102	42	0	3	104	1	102	2.02e-46	144
MS.gene050229.t1	AT5G18600	59.804	102	41	0	3	104	1	102	4.26e-46	144
MS.gene050229.t1	AT4G15680	57.843	102	43	0	3	104	1	102	1.19e-43	137
MS.gene050229.t1	AT4G15690	57.843	102	43	0	3	104	1	102	4.65e-43	136
MS.gene050229.t1	AT4G15700	56.863	102	44	0	3	104	1	102	5.24e-43	136
MS.gene050229.t1	AT4G15670	53.922	102	47	0	3	104	1	102	2.40e-41	132
MS.gene050229.t1	AT4G15660	54.902	102	46	0	3	104	1	102	7.35e-41	130
MS.gene050229.t1	AT3G21460	48.039	102	53	0	3	104	1	102	2.19e-33	112
MS.gene050229.t1	AT3G62950	45.631	103	55	1	3	104	1	103	2.69e-29	101
MS.gene050229.t1	AT2G47870	40.777	103	60	1	3	104	1	103	4.27e-28	98.6
MS.gene050229.t1	AT1G06830	43.617	94	53	0	3	96	1	94	4.69e-28	98.2
MS.gene050229.t1	AT2G30540	43.617	94	53	0	3	96	1	94	9.84e-28	97.4
MS.gene050229.t1	AT2G47880	41.489	94	55	0	3	96	1	94	1.69e-26	94.4
MS.gene050229.t1	AT5G14070	42.991	107	53	2	6	104	34	140	6.02e-26	94.4
MS.gene050229.t1	AT3G62960	40.426	94	56	0	3	96	1	94	3.15e-25	91.3
MS.gene050229.t1	AT3G02000	43.810	105	53	1	6	104	32	136	3.39e-23	87.0
MS.gene050229.t1	AT5G63030	40.196	102	61	0	1	102	17	118	1.38e-20	80.1
MS.gene050229.t1	AT4G33040	37.864	103	60	1	6	104	42	144	2.47e-18	74.7
MS.gene050229.t1	AT5G40370	39.216	102	62	0	1	102	1	102	3.47e-18	73.6
MS.gene050229.t1	AT1G28480	31.429	105	68	2	4	104	33	137	6.64e-17	70.9
MS.gene050229.t1	AT2G20270	34.653	101	65	1	2	101	74	174	1.69e-16	70.9
MS.gene050229.t1	AT5G11930	34.343	99	55	3	6	103	58	147	1.93e-16	70.1
MS.gene050229.t1	AT1G03850	32.292	96	61	1	9	104	59	150	4.13e-15	66.6
MS.gene050229.t1	AT5G40370	40.698	86	51	0	17	102	42	127	1.12e-13	62.8
MS.gene050229.t1	AT4G28730	33.333	99	65	1	4	101	71	169	1.67e-12	60.5
MS.gene050229.t1	AT5G20500	32.990	97	65	0	4	100	33	129	3.93e-12	58.5

Find 27 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTGTACCAGTTGTGTTTAT+TGG	0.272819	1.4:-70615747	MS.gene050229:CDS
TCATCAAGCTCATAAACAAT+TGG	0.354952	1.4:+70615825	None:intergenic
TCATGAATGCCGGAGCTATA+TGG	0.387749	1.4:-70615656	MS.gene050229:CDS
TAGAGCCACCAATGAATTGT+TGG	0.395335	1.4:+70615724	None:intergenic
GAGCTTGATGAGATAACAAA+TGG	0.402669	1.4:-70615813	MS.gene050229:CDS
CATCAAGCTCATAAACAATT+GGG	0.430944	1.4:+70615826	None:intergenic
GAATCATGACCCTTCATCTT+CGG	0.437884	1.4:-70615698	MS.gene050229:CDS
TGTTGGCCAATAAACACAAC+TGG	0.465473	1.4:+70615741	None:intergenic
TTTATTGGCCAACAATTCAT+TGG	0.465893	1.4:-70615732	MS.gene050229:CDS
AAAATAATTCTAGCTAATCA+TGG	0.472950	1.4:-70615955	None:intergenic
ATGACATTGATACGAAGCTT+TGG	0.484478	1.4:-70615855	MS.gene050229:CDS
CTAGCTCATTCCGAAGATGA+AGG	0.506749	1.4:+70615688	None:intergenic
ATTGGCCAACAATTCATTGG+TGG	0.532087	1.4:-70615729	MS.gene050229:CDS
AAATTAAATCCATATAGCTC+CGG	0.538653	1.4:+70615647	None:intergenic
GAAAGAGCACTGATTCAACT+AGG	0.544425	1.4:-70615780	MS.gene050229:CDS
ACACAACTGGTACACTTGGT+TGG	0.550739	1.4:+70615754	None:intergenic
GTACCTATGCTCATGAATGC+CGG	0.552236	1.4:-70615666	MS.gene050229:CDS
CTCTTGCTGAATATCACCAC+AGG	0.554486	1.4:+70615903	None:intergenic
ATAAACACAACTGGTACACT+TGG	0.565056	1.4:+70615750	None:intergenic
GCTTCGTATCAATGTCATGA+TGG	0.581724	1.4:+70615860	None:intergenic
AGCTTGATGAGATAACAAAT+GGG	0.597901	1.4:-70615812	MS.gene050229:CDS
TAATCATGGACATGATTACA+AGG	0.612323	1.4:-70615941	MS.gene050229:CDS
TAGCTCATTCCGAAGATGAA+GGG	0.619643	1.4:+70615689	None:intergenic
AAAGAGCACTGATTCAACTA+GGG	0.626942	1.4:-70615779	MS.gene050229:CDS
GTATCAATGTCATGATGGAG+TGG	0.644776	1.4:+70615865	None:intergenic
GCTCCGGCATTCATGAGCAT+AGG	0.659701	1.4:+70615663	None:intergenic
GATAGTGAGTGATAAACCTG+TGG	0.751670	1.4:-70615919	MS.gene050229:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGCTTGATGAGATAACAAAT+GGG	-	chr1.4:70615780-70615799	MS.gene050229:CDS	30.0%
	CATCAAGCTCATAAACAATT+GGG	+	chr1.4:70615769-70615788	None:intergenic	30.0%
	TAATCATGGACATGATTACA+AGG	-	chr1.4:70615651-70615670	MS.gene050229:CDS	30.0%
	TCATCAAGCTCATAAACAAT+TGG	+	chr1.4:70615770-70615789	None:intergenic	30.0%
	TTTATTGGCCAACAATTCAT+TGG	-	chr1.4:70615860-70615879	MS.gene050229:CDS	30.0%
	AGTGTACCAGTTGTGTTTAT+TGG	-	chr1.4:70615845-70615864	MS.gene050229:CDS	35.0%
	ATAAACACAACTGGTACACT+TGG	+	chr1.4:70615845-70615864	None:intergenic	35.0%
	ATGACATTGATACGAAGCTT+TGG	-	chr1.4:70615737-70615756	MS.gene050229:CDS	35.0%
	GAGCTTGATGAGATAACAAA+TGG	-	chr1.4:70615779-70615798	MS.gene050229:CDS	35.0%
!!	AAAGAGCACTGATTCAACTA+GGG	-	chr1.4:70615813-70615832	MS.gene050229:CDS	35.0%
	ATTGGCCAACAATTCATTGG+TGG	-	chr1.4:70615863-70615882	MS.gene050229:CDS	40.0%
	GAATCATGACCCTTCATCTT+CGG	-	chr1.4:70615894-70615913	MS.gene050229:CDS	40.0%
	GATAGTGAGTGATAAACCTG+TGG	-	chr1.4:70615673-70615692	MS.gene050229:CDS	40.0%
	GCTTCGTATCAATGTCATGA+TGG	+	chr1.4:70615735-70615754	None:intergenic	40.0%
	TAGAGCCACCAATGAATTGT+TGG	+	chr1.4:70615871-70615890	None:intergenic	40.0%
	TAGCTCATTCCGAAGATGAA+GGG	+	chr1.4:70615906-70615925	None:intergenic	40.0%
	TGTTGGCCAATAAACACAAC+TGG	+	chr1.4:70615854-70615873	None:intergenic	40.0%
!	GTATCAATGTCATGATGGAG+TGG	+	chr1.4:70615730-70615749	None:intergenic	40.0%
!!	GAAAGAGCACTGATTCAACT+AGG	-	chr1.4:70615812-70615831	MS.gene050229:CDS	40.0%
	ACACAACTGGTACACTTGGT+TGG	+	chr1.4:70615841-70615860	None:intergenic	45.0%
	CTAGCTCATTCCGAAGATGA+AGG	+	chr1.4:70615907-70615926	None:intergenic	45.0%
	CTCTTGCTGAATATCACCAC+AGG	+	chr1.4:70615692-70615711	None:intergenic	45.0%
	GTACCTATGCTCATGAATGC+CGG	-	chr1.4:70615926-70615945	MS.gene050229:CDS	45.0%
	TCATGAATGCCGGAGCTATA+TGG	-	chr1.4:70615936-70615955	MS.gene050229:CDS	45.0%
	GCTCCGGCATTCATGAGCAT+AGG	+	chr1.4:70615932-70615951	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	70615650	70615964	70615650	ID=MS.gene050229
chr1.4	mRNA	70615650	70615964	70615650	ID=MS.gene050229.t1;Parent=MS.gene050229
chr1.4	exon	70615650	70615964	70615650	ID=MS.gene050229.t1.exon1;Parent=MS.gene050229.t1
chr1.4	CDS	70615650	70615964	70615650	ID=cds.MS.gene050229.t1;Parent=MS.gene050229.t1

Gene Sequence	>MS.gene050229 CTAATCATGGACATGATTACAAGGATAGTGAGTGATAAACCTGTGGTGATATTCAGCAAGAGCACATGTTGTTTGAGCCACTCCATCATGACATTGATACGAAGCTTTGGTGCAAACCCAATTGTTTATGAGCTTGATGAGATAACAAATGGGTCACAGATAGAAAGAGCACTGATTCAACTAGGGTGCCAACCAAGTGTACCAGTTGTGTTTATTGGCCAACAATTCATTGGTGGCTCTAAAAGAATCATGACCCTTCATCTTCGGAATGAGCTAGTACCTATGCTCATGAATGCCGGAGCTATATGGATTTAA
Protein sequence	>MS.gene050229.t1 LIMDMITRIVSDKPVVIFSKSTCCLSHSIMTLIRSFGANPIVYELDEITNGSQIERALIQLGCQPSVPVVFIGQQFIGGSKRIMTLHLRNELVPMLMNAGAIWI