AlfalfaGEDB | MS.gene20701

M. sativa cultivar XinJiangDaYe / MS.gene20701

KEGG: K03676
GO: GO:0003674 GO:0003824 GO:0008150 GO:0009055 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0019725 GO:0042592 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008
PFAM: PF00462.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20701.t1	XP_013469215.1	99	99	1	0	1	99	1	99	1.90E-48	201.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20701.t1	O82255	72.4	98	27	0	1	98	1	98	2.0e-38	159.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20701.t1	A0A072VPA8	99.0	99	1	0	1	99	1	99	1.4e-48	201.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene041521	MS.gene20701	PPI
MS.gene07181	MS.gene20701	PPI
MS.gene006982	MS.gene20701	PPI
MS.gene23007	MS.gene20701	PPI
MS.gene20701	MS.gene07181	PPI
MS.gene58938	MS.gene20701	PPI
MS.gene23008	MS.gene20701	PPI
MS.gene036524	MS.gene20701	PPI
MS.gene03022	MS.gene20701	PPI
MS.gene022312	MS.gene20701	PPI
MS.gene70642	MS.gene20701	PPI
MS.gene005628	MS.gene20701	PPI
MS.gene70641	MS.gene20701	PPI
MS.gene005632	MS.gene20701	PPI
MS.gene07179	MS.gene20701	PPI
MS.gene20701	MS.gene005628	PPI
MS.gene23009	MS.gene20701	PPI
MS.gene041519	MS.gene20701	PPI
MS.gene005631	MS.gene20701	PPI
MS.gene035291	MS.gene20701	PPI
MS.gene23005	MS.gene20701	PPI
MS.gene29370	MS.gene20701	PPI
MS.gene58937	MS.gene20701	PPI
MS.gene29371	MS.gene20701	PPI
MS.gene03023	MS.gene20701	PPI
MS.gene035294	MS.gene20701	PPI
MS.gene58935	MS.gene20701	PPI
MS.gene07180	MS.gene20701	PPI
MS.gene050229	MS.gene20701	PPI
MS.gene036528	MS.gene20701	PPI
MS.gene03026	MS.gene20701	PPI
MS.gene25978	MS.gene20701	PPI
MS.gene006983	MS.gene20701	PPI
MS.gene005630	MS.gene20701	PPI
MS.gene006980	MS.gene20701	PPI
MS.gene041518	MS.gene20701	PPI
MS.gene20704	MS.gene20701	PPI
MS.gene07182	MS.gene20701	PPI
MS.gene52509	MS.gene20701	PPI
MS.gene20701	MS.gene005631	PPI
MS.gene035290	MS.gene20701	PPI
MS.gene87167	MS.gene20701	PPI
MS.gene20705	MS.gene20701	PPI
MS.gene022311	MS.gene20701	PPI
MS.gene036527	MS.gene20701	PPI
MS.gene59643	MS.gene20701	PPI
MS.gene005629	MS.gene20701	PPI
MS.gene03024	MS.gene20701	PPI
MS.gene005633	MS.gene20701	PPI
MS.gene29979	MS.gene20701	PPI
MS.gene006984	MS.gene20701	PPI
MS.gene20702	MS.gene20701	PPI
MS.gene20703	MS.gene20701	PPI
MS.gene20701	MS.gene006982	PPI
MS.gene07183	MS.gene20701	PPI
MS.gene25976	MS.gene20701	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20701.t1	MTR_1g088925	98.990	99	1	0	1	99	1	99	4.47e-69	201
MS.gene20701.t1	MTR_1g088920	95.960	99	4	0	1	99	1	99	4.02e-68	199
MS.gene20701.t1	MTR_7g108200	76.531	98	23	0	1	98	1	98	3.46e-57	171
MS.gene20701.t1	MTR_2g019900	56.842	95	41	0	1	95	1	95	5.09e-39	125
MS.gene20701.t1	MTR_1g088910	52.041	98	47	0	1	98	1	98	6.10e-36	118
MS.gene20701.t1	MTR_2g019950	54.737	95	43	0	1	95	1	95	1.37e-35	117
MS.gene20701.t1	MTR_7g022690	51.042	96	46	1	1	95	30	125	6.40e-34	114
MS.gene20701.t1	MTR_7g022710	50.000	96	47	1	1	95	30	125	1.89e-33	112
MS.gene20701.t1	MTR_7g022550	50.000	96	47	1	1	95	30	125	1.54e-32	110
MS.gene20701.t1	MTR_4g119050	50.000	96	47	1	1	95	1	96	7.39e-32	107
MS.gene20701.t1	MTR_4g119030	50.000	96	47	1	1	95	1	96	7.39e-32	107
MS.gene20701.t1	MTR_4g119040	50.000	96	47	1	1	95	1	96	7.39e-32	107
MS.gene20701.t1	MTR_7g026770	51.042	96	46	1	1	95	22	117	4.00e-31	111
MS.gene20701.t1	MTR_7g108220	49.474	95	47	1	1	94	1	95	1.65e-30	104
MS.gene20701.t1	MTR_7g108210	48.421	95	48	1	1	94	1	95	1.11e-29	102
MS.gene20701.t1	MTR_7g108250	41.053	95	56	0	1	95	1	95	9.43e-26	92.4
MS.gene20701.t1	MTR_1g088905	38.298	94	58	0	1	94	1	94	5.60e-25	90.5
MS.gene20701.t1	MTR_1g088895	42.857	98	56	0	1	98	1	98	1.84e-21	81.6
MS.gene20701.t1	MTR_2g048970	39.796	98	55	1	2	95	29	126	3.53e-21	81.6
MS.gene20701.t1	MTR_7g108260	40.426	94	56	0	1	94	1	94	3.30e-20	78.2
MS.gene20701.t1	MTR_2g090755	38.542	96	52	2	4	95	41	133	1.50e-19	77.8
MS.gene20701.t1	MTR_2g014760	36.170	94	60	0	2	95	27	120	8.30e-19	75.5
MS.gene20701.t1	MTR_3g077570	35.789	95	61	0	1	95	18	112	1.30e-16	69.7
MS.gene20701.t1	MTR_4g079110	38.710	93	52	3	6	95	58	148	5.32e-16	68.9
MS.gene20701.t1	MTR_3g077560	34.043	94	62	0	1	94	21	114	6.28e-16	68.2
MS.gene20701.t1	MTR_7g035245	37.234	94	59	0	1	94	3	96	9.98e-16	67.0
MS.gene20701.t1	MTR_1g015890	36.170	94	58	1	3	94	48	141	7.35e-14	63.2
MS.gene20701.t1	MTR_1g069255	32.979	94	62	1	2	94	84	177	8.24e-14	63.9
MS.gene20701.t1	MTR_3g104510	35.484	93	54	3	3	94	23	110	3.96e-13	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20701.t1	AT2G47880	72.449	98	27	0	1	98	1	98	1.96e-52	159
MS.gene20701.t1	AT1G06830	73.196	97	26	0	1	97	1	97	8.11e-52	158
MS.gene20701.t1	AT2G30540	66.327	98	33	0	1	98	1	98	2.61e-51	157
MS.gene20701.t1	AT3G62960	70.408	98	29	0	1	98	1	98	8.16e-51	155
MS.gene20701.t1	AT3G62950	51.042	96	46	1	1	95	1	96	1.97e-36	119
MS.gene20701.t1	AT2G47870	54.167	96	43	1	1	95	1	96	6.37e-36	118
MS.gene20701.t1	AT4G15670	50.526	95	47	0	1	95	1	95	2.92e-34	114
MS.gene20701.t1	AT4G15680	51.579	95	46	0	1	95	1	95	1.02e-33	112
MS.gene20701.t1	AT4G15660	49.485	97	49	0	1	97	1	97	1.06e-33	112
MS.gene20701.t1	AT4G15700	51.579	95	46	0	1	95	1	95	1.16e-33	112
MS.gene20701.t1	AT3G21460	53.684	95	44	0	1	95	1	95	1.27e-33	112
MS.gene20701.t1	AT4G15690	50.526	95	47	0	1	95	1	95	5.15e-33	110
MS.gene20701.t1	AT5G18600	46.316	95	51	0	1	95	1	95	7.74e-31	105
MS.gene20701.t1	AT5G14070	42.718	103	51	2	1	95	31	133	8.50e-26	93.6
MS.gene20701.t1	AT3G62930	41.053	95	56	0	1	95	1	95	1.51e-25	92.0
MS.gene20701.t1	AT1G03020	43.158	95	54	0	1	95	1	95	8.14e-25	90.1
MS.gene20701.t1	AT3G02000	42.424	99	51	1	3	95	31	129	2.33e-24	89.7
MS.gene20701.t1	AT1G28480	36.735	98	58	2	2	95	33	130	2.29e-17	72.0
MS.gene20701.t1	AT4G33040	39.175	97	53	2	3	94	41	136	3.02e-16	69.3
MS.gene20701.t1	AT5G40370	35.106	94	61	0	1	94	3	96	1.02e-15	67.0
MS.gene20701.t1	AT5G11930	41.935	93	44	4	3	94	57	140	5.11e-15	66.2
MS.gene20701.t1	AT4G28730	31.915	94	63	1	2	94	71	164	1.19e-13	63.2
MS.gene20701.t1	AT5G63030	37.805	82	51	0	13	94	31	112	3.54e-13	60.8
MS.gene20701.t1	AT5G20500	29.670	91	64	0	4	94	35	125	2.74e-11	56.2
MS.gene20701.t1	AT2G20270	31.915	94	63	1	2	94	76	169	2.76e-11	57.0
MS.gene20701.t1	AT1G03850	34.884	86	52	2	10	95	62	143	4.53e-11	56.2

Find 35 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGGGTACACTCCAATTTCT+TGG	0.312373	1.2:-66682730	None:intergenic
TTCTCCATTTCTTTCCCATC+AGG	0.358196	1.2:-66682776	None:intergenic
GGAGAAAGCTATAACAAAGT+TGG	0.418322	1.2:+66682793	MS.gene20701:CDS
AGATCCTGATGGGAAAGAAA+TGG	0.430695	1.2:+66682772	MS.gene20701:CDS
CCTGTCCCTGCAGTATTCAT+TGG	0.431206	1.2:+66682827	MS.gene20701:CDS
GTGTAGGGACATTACTTCAT+TGG	0.438643	1.2:-66682870	None:intergenic
TGATCAAAGTAAGCTAAATT+CGG	0.447173	1.2:-66682928	None:intergenic
GAGAAAGCTATAACAAAGTT+GGG	0.448427	1.2:+66682794	MS.gene20701:CDS
TTGTCAATTTCATGAATCAC+AGG	0.463222	1.2:-66682749	None:intergenic
CTTCCTCCAATGAATACTGC+AGG	0.488474	1.2:-66682833	None:intergenic
GATGAGGCTAGCTACAGAAA+AGG	0.500022	1.2:+66682649	MS.gene20701:CDS
GCATAGACAACAAGAGCTCT+TGG	0.505172	1.2:-66682687	None:intergenic
TCATTGGAGGAAGGCTGGTG+GGG	0.511910	1.2:+66682843	MS.gene20701:CDS
TTCATTGGAGGAAGGCTGGT+GGG	0.512409	1.2:+66682842	MS.gene20701:CDS
CCAATGAATACTGCAGGGAC+AGG	0.522380	1.2:-66682827	None:intergenic
GTTCACTTGTTCCACTACTC+AGG	0.536048	1.2:+66682900	MS.gene20701:CDS
AGTATTCATTGGAGGAAGGC+TGG	0.536333	1.2:+66682838	MS.gene20701:CDS
ATGAGGCTAGCTACAGAAAA+GGG	0.537218	1.2:+66682650	MS.gene20701:CDS
GTCCCTGCAGTATTCATTGG+AGG	0.543693	1.2:+66682830	MS.gene20701:CDS
GAAATTGACAAAGATCCTGA+TGG	0.544778	1.2:+66682761	MS.gene20701:CDS
AAAGTAAGCTAAATTCGGTA+TGG	0.551896	1.2:-66682923	None:intergenic
TAGGGACATTACTTCATTGG+TGG	0.552920	1.2:-66682867	None:intergenic
ATTCGGTATGGCCTGAGTAG+TGG	0.556723	1.2:-66682911	None:intergenic
CAAGTGAACCACTTAGGTGT+AGG	0.571843	1.2:-66682886	None:intergenic
ATTCATTGGAGGAAGGCTGG+TGG	0.573512	1.2:+66682841	MS.gene20701:CDS
AAGTGAACCACTTAGGTGTA+GGG	0.580489	1.2:-66682885	None:intergenic
GTGGAACAAGTGAACCACTT+AGG	0.593219	1.2:-66682892	None:intergenic
AGCTACAGAAAAGGGTGTGG+TGG	0.593409	1.2:+66682658	MS.gene20701:CDS
CTGCAGTATTCATTGGAGGA+AGG	0.598971	1.2:+66682834	MS.gene20701:CDS
GTAATGTCCCTACACCTAAG+TGG	0.609456	1.2:+66682878	MS.gene20701:CDS
AAATTGACAAAGATCCTGAT+GGG	0.612548	1.2:+66682762	MS.gene20701:CDS
TTCCTCCAATGAATACTGCA+GGG	0.637919	1.2:-66682832	None:intergenic
GCTAGCTACAGAAAAGGGTG+TGG	0.645043	1.2:+66682655	MS.gene20701:CDS
TGTCAATTTCATGAATCACA+GGG	0.646979	1.2:-66682748	None:intergenic
TTAAGATGGAGAAGGTGATG+AGG	0.659848	1.2:+66682633	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACATTTTGTTCCAAGAAAT+TGG	+	chr1.2:66682719-66682738	MS.gene20701:CDS	25.0%
	AAATTGACAAAGATCCTGAT+GGG	+	chr1.2:66682762-66682781	MS.gene20701:CDS	30.0%
	GAGAAAGCTATAACAAAGTT+GGG	+	chr1.2:66682794-66682813	MS.gene20701:CDS	30.0%
	TGTCAATTTCATGAATCACA+GGG	-	chr1.2:66682751-66682770	None:intergenic	30.0%
	TTGTCAATTTCATGAATCAC+AGG	-	chr1.2:66682752-66682771	None:intergenic	30.0%
	GAAATTGACAAAGATCCTGA+TGG	+	chr1.2:66682761-66682780	MS.gene20701:CDS	35.0%
	GGAGAAAGCTATAACAAAGT+TGG	+	chr1.2:66682793-66682812	MS.gene20701:CDS	35.0%
	AAGTGAACCACTTAGGTGTA+GGG	-	chr1.2:66682888-66682907	None:intergenic	40.0%
	AGATCCTGATGGGAAAGAAA+TGG	+	chr1.2:66682772-66682791	MS.gene20701:CDS	40.0%
	GTGTAGGGACATTACTTCAT+TGG	-	chr1.2:66682873-66682892	None:intergenic	40.0%
	TAGGGACATTACTTCATTGG+TGG	-	chr1.2:66682870-66682889	None:intergenic	40.0%
	TTCCTCCAATGAATACTGCA+GGG	-	chr1.2:66682835-66682854	None:intergenic	40.0%
	TTCTCCATTTCTTTCCCATC+AGG	-	chr1.2:66682779-66682798	None:intergenic	40.0%
!	ATGAGGCTAGCTACAGAAAA+GGG	+	chr1.2:66682650-66682669	MS.gene20701:CDS	40.0%
	AGTATTCATTGGAGGAAGGC+TGG	+	chr1.2:66682838-66682857	MS.gene20701:CDS	45.0%
	CAAGTGAACCACTTAGGTGT+AGG	-	chr1.2:66682889-66682908	None:intergenic	45.0%
	CAGGGTACACTCCAATTTCT+TGG	-	chr1.2:66682733-66682752	None:intergenic	45.0%
	CTGCAGTATTCATTGGAGGA+AGG	+	chr1.2:66682834-66682853	MS.gene20701:CDS	45.0%
	CTTCCTCCAATGAATACTGC+AGG	-	chr1.2:66682836-66682855	None:intergenic	45.0%
	GCATAGACAACAAGAGCTCT+TGG	-	chr1.2:66682690-66682709	None:intergenic	45.0%
	GTAATGTCCCTACACCTAAG+TGG	+	chr1.2:66682878-66682897	MS.gene20701:CDS	45.0%
	GTGGAACAAGTGAACCACTT+AGG	-	chr1.2:66682895-66682914	None:intergenic	45.0%
	GTTCACTTGTTCCACTACTC+AGG	+	chr1.2:66682900-66682919	MS.gene20701:CDS	45.0%
!	GATGAGGCTAGCTACAGAAA+AGG	+	chr1.2:66682649-66682668	MS.gene20701:CDS	45.0%
	ATTCATTGGAGGAAGGCTGG+TGG	+	chr1.2:66682841-66682860	MS.gene20701:CDS	50.0%
	ATTCGGTATGGCCTGAGTAG+TGG	-	chr1.2:66682914-66682933	None:intergenic	50.0%
	CCAATGAATACTGCAGGGAC+AGG	-	chr1.2:66682830-66682849	None:intergenic	50.0%
	CCTGTCCCTGCAGTATTCAT+TGG	+	chr1.2:66682827-66682846	MS.gene20701:CDS	50.0%
	GCTAGCTACAGAAAAGGGTG+TGG	+	chr1.2:66682655-66682674	MS.gene20701:CDS	50.0%
	GTCCCTGCAGTATTCATTGG+AGG	+	chr1.2:66682830-66682849	MS.gene20701:CDS	50.0%
	TTCATTGGAGGAAGGCTGGT+GGG	+	chr1.2:66682842-66682861	MS.gene20701:CDS	50.0%
!	AGCTACAGAAAAGGGTGTGG+TGG	+	chr1.2:66682658-66682677	MS.gene20701:CDS	50.0%
	TCATTGGAGGAAGGCTGGTG+GGG	+	chr1.2:66682843-66682862	MS.gene20701:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	66682638	66682937	66682638	ID=MS.gene20701
chr1.2	mRNA	66682638	66682937	66682638	ID=MS.gene20701.t1;Parent=MS.gene20701
chr1.2	exon	66682638	66682937	66682638	ID=MS.gene20701.t1.exon1;Parent=MS.gene20701.t1
chr1.2	CDS	66682638	66682937	66682638	ID=cds.MS.gene20701.t1;Parent=MS.gene20701.t1

Gene Sequence	>MS.gene20701 ATGGAGAAGGTGATGAGGCTAGCTACAGAAAAGGGTGTGGTGGTATTCACCAAGAGCTCTTGTTGTCTATGCTATGCAGTGAACATTTTGTTCCAAGAAATTGGAGTGTACCCTGTGATTCATGAAATTGACAAAGATCCTGATGGGAAAGAAATGGAGAAAGCTATAACAAAGTTGGGTTGTAATGCACCTGTCCCTGCAGTATTCATTGGAGGAAGGCTGGTGGGGTCCACCAATGAAGTAATGTCCCTACACCTAAGTGGTTCACTTGTTCCACTACTCAGGCCATACCGAATTTAG
Protein sequence	>MS.gene20701.t1 MEKVMRLATEKGVVVFTKSSCCLCYAVNILFQEIGVYPVIHEIDKDPDGKEMEKAITKLGCNAPVPAVFIGGRLVGSTNEVMSLHLSGSLVPLLRPYRI