AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25978


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25978.t1 MTR_3g077560 91.339 127 5 1 1 121 1 127 4.12e-82 236
MS.gene25978.t1 MTR_3g077570 76.667 120 19 2 1 114 1 117 2.86e-63 188
MS.gene25978.t1 MTR_7g035245 53.333 105 43 1 21 119 3 107 8.71e-37 121
MS.gene25978.t1 MTR_1g069255 46.000 100 45 2 25 117 87 184 4.98e-23 88.6
MS.gene25978.t1 MTR_2g038560 40.000 100 47 2 15 108 29 121 5.75e-19 76.6
MS.gene25978.t1 MTR_2g038560 40.000 100 47 2 15 108 70 162 8.15e-19 77.4
MS.gene25978.t1 MTR_2g038560 40.000 100 47 2 15 108 73 165 8.69e-19 77.4
MS.gene25978.t1 MTR_2g019900 35.000 100 59 1 21 114 1 100 2.60e-14 63.9
MS.gene25978.t1 MTR_5g021090 38.824 85 46 1 30 108 42 126 3.47e-14 64.3
MS.gene25978.t1 MTR_1g088905 32.000 100 62 1 21 114 1 100 1.24e-13 62.4
MS.gene25978.t1 MTR_7g022690 37.500 104 58 2 18 114 27 130 1.36e-13 63.2
MS.gene25978.t1 MTR_7g022710 37.500 104 58 2 18 114 27 130 5.57e-13 61.2
MS.gene25978.t1 MTR_7g022550 36.538 104 59 2 18 114 27 130 9.50e-13 60.8
MS.gene25978.t1 MTR_7g108250 33.000 100 61 1 21 114 1 100 1.25e-12 59.7
MS.gene25978.t1 MTR_4g119050 30.693 101 63 1 21 114 1 101 4.40e-12 58.2
MS.gene25978.t1 MTR_4g119030 30.693 101 63 1 21 114 1 101 4.40e-12 58.2
MS.gene25978.t1 MTR_4g119040 30.693 101 63 1 21 114 1 101 4.40e-12 58.2
MS.gene25978.t1 MTR_7g108210 32.673 101 61 2 21 114 1 101 4.84e-12 58.2
MS.gene25978.t1 MTR_7g108220 32.673 101 61 2 21 114 1 101 5.34e-12 58.2
MS.gene25978.t1 MTR_2g014760 34.343 99 58 3 22 114 28 125 2.15e-11 57.0
MS.gene25978.t1 MTR_7g026770 34.862 109 64 2 13 114 14 122 7.59e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25978.t1 AT5G63030 61.417 127 41 2 1 121 1 125 5.32e-53 162
MS.gene25978.t1 AT5G40370 58.095 105 38 1 21 119 3 107 3.45e-40 130
MS.gene25978.t1 AT5G40370 59.341 91 31 1 35 119 42 132 2.27e-34 116
MS.gene25978.t1 AT2G20270 45.000 100 47 2 20 111 73 172 1.17e-20 82.4
MS.gene25978.t1 AT5G20500 38.947 95 52 1 24 112 35 129 2.99e-19 77.8
MS.gene25978.t1 AT4G28730 39.423 104 53 2 16 112 68 168 1.99e-18 76.6
MS.gene25978.t1 AT1G77370 41.860 86 44 1 30 109 43 128 3.07e-16 69.7
MS.gene25978.t1 AT2G20270 35.433 127 47 2 20 111 73 199 4.09e-16 71.2
MS.gene25978.t1 AT5G14070 35.780 109 56 2 20 114 30 138 2.91e-15 67.4
MS.gene25978.t1 AT4G33040 32.203 118 70 3 7 114 25 142 1.13e-13 63.5
MS.gene25978.t1 AT5G18600 34.000 100 60 1 21 114 1 100 6.70e-13 60.5
MS.gene25978.t1 AT1G77370 32.432 111 43 2 30 108 43 153 1.66e-12 60.8
MS.gene25978.t1 AT5G11930 32.673 101 64 2 15 114 49 146 1.21e-11 58.2
MS.gene25978.t1 AT1G03020 35.000 100 59 1 21 114 1 100 2.38e-11 56.6
MS.gene25978.t1 AT3G02000 34.862 109 59 2 18 114 26 134 2.70e-11 57.0
MS.gene25978.t1 AT4G15700 35.354 99 57 2 22 114 3 100 6.39e-11 55.5

Find 33 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTGTTTCCATCTCTTCTT+TGG 0.212250 3.3:-65816767 None:intergenic
ACAGTACCTAATGTGTTTAT+TGG 0.265313 3.3:+65823697 MS.gene25978:CDS
TTTATTTGTTGCAGCTGTTT+TGG 0.272729 3.3:+65823858 MS.gene25978:intron
TCAGAGCTGAGCAGAGTTCT+TGG 0.303817 3.3:-65823935 None:intergenic
CATCATTTCAAGGACCTTGT+AGG 0.332359 3.3:-65817380 None:intergenic
CTAAAACTTTCATCATTTCA+AGG 0.373897 3.3:-65817390 None:intergenic
CTTATTGTGGTTACTGCAAA+CGG 0.407824 3.3:+65817326 MS.gene25978:CDS
CAAATAATGGGTTCTATGCT+TGG 0.415863 3.3:+65816724 None:intergenic
ATTCCCCTTCTCACTGATGC+TGG 0.424957 3.3:+65823904 MS.gene25978:CDS
TTGCCTCCAATAAACACATT+AGG 0.466203 3.3:-65823703 None:intergenic
CGCTCCAGCATCAGTGAGAA+GGG 0.479868 3.3:-65823908 None:intergenic
TTATTGTGGTTACTGCAAAC+GGG 0.502636 3.3:+65817327 MS.gene25978:CDS
AATAACCTGAAGACAAAGAC+GGG 0.504868 3.3:-65816823 None:intergenic
TGGTTACTGCAAACGGGTCA+AGG 0.522187 3.3:+65817333 MS.gene25978:CDS
GTACCTAATGTGTTTATTGG+AGG 0.525018 3.3:+65823700 MS.gene25978:CDS
GCAGCATTAACTGAGTGGAC+AGG 0.525614 3.3:+65823667 MS.gene25978:CDS
ATTGGAGGCAAGCACATTGG+TGG 0.528449 3.3:+65823715 MS.gene25978:CDS
TATGACAGTAAGACTTATTG+TGG 0.543804 3.3:+65817313 MS.gene25978:intron
TCAAGGGACCAAAGAAGAGA+TGG 0.547018 3.3:+65816759 MS.gene25978:CDS
TCGCTCCAGCATCAGTGAGA+AGG 0.547446 3.3:-65823909 None:intergenic
CTTGGTTCTCAGAAGACTCA+AGG 0.550504 3.3:+65816742 MS.gene25978:CDS
CAGCATTAACTGAGTGGACA+GGG 0.563697 3.3:+65823668 MS.gene25978:CDS
TAACTGAGTGGACAGGGCAA+AGG 0.599599 3.3:+65823674 MS.gene25978:CDS
AAGGATCTACTCAAACAGCT+AGG 0.624288 3.3:+65817352 MS.gene25978:CDS
AAATAACCTGAAGACAAAGA+CGG 0.625074 3.3:-65816824 None:intergenic
TTGGTTCTCAGAAGACTCAA+GGG 0.625272 3.3:+65816743 MS.gene25978:CDS
ACAGCTAGGAGCAACCTACA+AGG 0.634355 3.3:+65817366 MS.gene25978:CDS
TTTATTGGAGGCAAGCACAT+TGG 0.645273 3.3:+65823712 MS.gene25978:CDS
GGGGAATCAATTGACCTGCA+CGG 0.659613 3.3:-65823888 None:intergenic
GCTCCAGCATCAGTGAGAAG+GGG 0.673549 3.3:-65823907 None:intergenic
TTCATGCAGCATTAACTGAG+TGG 0.679113 3.3:+65823662 MS.gene25978:CDS
ATAACCTGAAGACAAAGACG+GGG 0.734989 3.3:-65816822 None:intergenic
TAACCTGAAGACAAAGACGG+GGG 0.745087 3.3:-65816821 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr3.3 gene 65816730 65823957 65816730 ID=MS.gene25978
chr3.3 mRNA 65816730 65823957 65816730 ID=MS.gene25978.t1;Parent=MS.gene25978
chr3.3 exon 65816730 65816839 65816730 ID=MS.gene25978.t1.exon1;Parent=MS.gene25978.t1
chr3.3 CDS 65816730 65816839 65816730 ID=cds.MS.gene25978.t1;Parent=MS.gene25978.t1
chr3.3 exon 65817321 65817405 65817321 ID=MS.gene25978.t1.exon2;Parent=MS.gene25978.t1
chr3.3 CDS 65817321 65817405 65817321 ID=cds.MS.gene25978.t1;Parent=MS.gene25978.t1
chr3.3 exon 65823661 65823745 65823661 ID=MS.gene25978.t1.exon3;Parent=MS.gene25978.t1
chr3.3 CDS 65823661 65823745 65823661 ID=cds.MS.gene25978.t1;Parent=MS.gene25978.t1
chr3.3 exon 65823872 65823957 65823872 ID=MS.gene25978.t1.exon4;Parent=MS.gene25978.t1
chr3.3 CDS 65823872 65823957 65823872 ID=cds.MS.gene25978.t1;Parent=MS.gene25978.t1
Gene Sequence

>MS.gene25978

ATGGGTTCTATGCTTGGTTCTCAGAAGACTCAAGGGACCAAAGAAGAGATGGAAACAACGTTGAATAAAACTAAACAGATTGCTGCCTCTTCCCCCGTCTTTGTCTTCAGGTTATTTTTATTTCTATCTAATCTTAGTAACATGTTTTTGTTGATCAAAATACTAGTTAAGGAGATGTCAATATTTTTTTAGGTAATAGGGGAATCTCGAAATAGAGAGACTATATTTATTTCTATATACTGTGCATGGAAGTTTAGAAATATGTCTTTTGATTTATGGTCAAGCCCGTGTTTGGTTTTGTAGTGTCACAAATTAATTTTGAATGAATTGATTATAGCTAAGAATGAATTGAATGTAAAAAGAAAATGAGTTGAAAAATAAATTCAAGGCTAAAAAAATAATTCTATAAAACTACATATTCTAGCTTCAAATTAGAATGAGGTAAAATTAGTTCTGCAACATGTATTGTATTGAATTGGTTGTTAAGGTATAGTTTGGTGTTTTTCACATGTAGCCCTCCTTGTTCATTTTCATAAAGCATTTTTTTGTTTTTAACCTTGATAATCCCATCTGTAATTTATGTTATGACAGTAAGACTTATTGTGGTTACTGCAAACGGGTCAAGGATCTACTCAAACAGCTAGGAGCAACCTACAAGGTCCTTGAAATGATGAAAGTTTTAGTGTTTAATCATGGATCACTAGATACAAATTATAGCATATCATCAATAACAAAAAAAGAACAAGAGATTGTGTACCTTTGAATTAGGTTGATGAAGGATAACCATAGACAACGCCGACAACTATGAGAGGGGTAACTACCCTCATTCCGTCTAGCTCTAGGTTTTCTCACCAATCTTCAGAATAATTAACCGTCTAATGGGATGACGTTTAAATAGCTAAACGGCTGAGGGCAGGGACCTCTCATTGGGCTTATCTAAATGGTCCAACCCATCACGGTCCATTTAGTAACAAAAGCCCAATCACTCAAATTCATCATATAATTAGTGCTTAACACAATAGACCATAATTTATATTATGACATCAACTTATAATAATTGATTATTGTCAATCCATAATTGATTAATTAAATAATTAATCCAACAATCTCCCACTTGGATGACAATACTCAATTACTGAAAAATAAAAAAATTCACTTGTCATAAAGAATTTAAAAATTTAAAAGTACATGTATGGTCAAAGCACTAATCATTCAAAAAAGAAGTAAGCTTTAATGCTGCAGAAATTGAATCCGATAGGGAAAACAAATCATACAGATCAAAGACTCACTTTTCTAACAAATGATGAATCTATAGTGACACGTTTGAATTAAAAAGAATATGTCACTAAAGAATAGCCCAAGCATTTTAAAAATGCTATAACAAACTCCCACCAACCCAAAATAACATAGACTTAATCAAATCATTATGAGTTACAATACTCATATATAAGTATGTGGTTAGTGGTTCTGCCAATGAGTTTTCAATTCCATAAAAGTCATATATCCACATAGGTATAAGAGTAGTAATTACTTATAGGGATAGCCTATAAAGAGATAGTAATTACATTTAGTCAAAGTCGATAAATGTTCTATGACATTCAATTAATCCAAGTTTTATAAAAAAAAAATGTGACTATGAAAATCCATAAAAGCAAAATAGGAAATTCAATAATCCCCAAATTGGATTAACATAGACACGTAGTTTAAAGAAAAATATGTGGATTAATGTGGCCACTAGAATGTCAATAACAAAAGAACATTTATGATTATCTCAAATTATAAGTACCACAAGAACACAATATCATACTCGATAGGAGTATTGATATTATACATTGTACTTACTATTTATGACCTCAAAAATGTGGAATGTTGGTAAAACAGAAACACAACGTATATTTGCATGCCAAGAAGTTATTGTGCATATTCCAACAAAATTGACTTAAGCATCCAATTATGATGCTCATTGAGATCGATAGAATTTTAACATATATCTCAATATAATAGAATGAGACTAAAGTTTAAAGTCTCTTAAGCTATGCCATATTTTATAGTGTGCACAAGAGTATATGGCGATAGCGACAATAATATTGTGTACACATTTCTCTTGATCTCACATATATATATAAACAAGAGTGAGATTTCTCATTTGAGCTCATAATATATAATATTGTGTACACATTCCCACTGATCCCACATATATATAAACAAGAGTAAGTGGAATTCTCATTAGTGATCACTGATACAAAATATTATAATAATGTAATATTCAACAGTGGGATACTCAATTACTAGTGCAAGATCATCAACATAAGTGGTTATATAATTTTAGCCAATAGAGTTTCATGAACTCAACCACATATTTTGCACATGAGCATAGCAGAATTATTAACACAAGTGGTAACTCAAAGATAATATGAAACCCCATAAATACATATTTTATCAATGGCAGATATTATCAAGAATTGGGTTTCGTAGTTAACCACATATTCTGCATTTACAATTAAGGAGAACGTAAATTTTCATTTAAGAATTTTATAACACAAGATCTCCTTTTCACAATTAAAATTCCACAGAATTTTATAAACATAGTAACCGTTATATGACAGAGTATGATGATTATATAAAAAATTTATCATCATATGAGTGAGATGAGTCTCTAAAAATAGGATATAAGACTCTGTTCACAATTTTAATAACTCACTCAAAACTCAAACGCTACAATTATACTTACACAAATTTGAGAAACAATTAGGTTACAAGCAGCGGAAATACACAAGACAAATAAATATATTTGTTTATGAGATATAGTTAGTTGACATGTAGAATTTTTCTCACTTGTTGAAGAATGATAGTTAAACTATCTTACCCACAAAAGGATGATTATGTTAAGTAATGTCATACCGATAGGGCATGTCCATCACGTTACACAATACATAGCAATGATTCTCCCACTTGATAGAGAATAGACAATGATAGTTGAATTAAAAAGGTTTGAAAGATATTTTAAATAAAATATTCAACTATTTCTCTATCACAAAATGATATGTTAAGTAACGTCACAGCGATAGGGTATGTCCGTCACTCACATAACAAAGGGATAGTAGCAATCCGATATATTTATATATATATAAAAATCACATTTAGGTAGAAATTTAATTCAAAATGTAATAATATTGGATTGCAAAATTTATACAATTTTCTACATGTCTTTTAACTATGTAACTTACTTATATTAATCTCATTCAAGGATCTTAACGATAATAAGTAAGAACTATATCATCACAATGTACCATTCTTATGTCTTACAAAATTTAAACATTGATAGAGTATTCTAATTATAATTGACATAAGTATAAACATTTTTGGTACATTGAATAATAACAATTATATTAATAACAACACACCGATAGGGTATGACTGTTACCAATATAATGCTAATCTATTTAACAAAATTTGCATAATTGTACCCACGATAGGTGAGATTACAATTATACAAATAAATAATGTTTATGCTTAAGAGAATATGATACAATAATATCATGCATAAACAATTATACTTAAATTTACTCAACTTCGGTCATATTCAAATGATAGAAAAATGTGCTTAACAATATTTTAAATTCACATCATAAATATGACTTTAGAATTTCTTTTATATGAAGTAGTATAATAACAAAAGTTGTTATTTGGAATTGAAGTGTGTCAAATAGTTTTCTAATTTATCCAGAAAAGAACTCTTAGAATAAAATTTTTCAATACTCCCACTATAAATTAGAAAAAAACACAAATACAAAATGGAGCATTATAGTAAGAAAATAGTATATAACAACAGAGTTCTGTTTAGAATATTCTGAAAAAGGATTTCACTTCTATATAGGATATCATAATTGCACAACAAAAGAGAAGAAGAACTAAATTTCTTCATATGTGCAATAATAAGCAATAAGTCACAAGAGGATAAAATAATTAAATAACAACTTACCTCGCAGAGTGTGACATTCATCCTTATACTTAGAGCCACTGAATTTTGATGACTTAGCGAGAATATCTTATAATATAGTCATCTCAAAAATATTCATCAATAGAGAATAAGCAATTATAAATAAGCATATGTGAGACTCTAATTATATATCAATATGATTTATATAAGAATTCAGATGTAAGGATGATACATATTTCAAAAATTGCCTCAATTCTTAATAAGTTGTTTTGATAATAATTAACGGATAAACAACAAAGAGACGTACAAATTTTAATTCATAATTAGAATTTTAGCATAAAAATGCAACAATGCATTATTCACTATATATAAAATATATAGTTTAATATTATTGTGATCCAAACATTTAATTGCGACAAGACACATAATTATATAGTTAATGGTGTTTGTCACAAAATTTAGAGTAATAAACGACGGGTCTAGATTAGAGAGTATAGTAGAAAGCATAATGTATATTAAAGAGAAATATGCCAATTTTAGATTCAATTAATATAAATGACATATATATACAACTCACTATACTCATTTTGAAAACAAAATATTATGAAGACCCTTTAAAACAATTTGTGACAAAAAATCAAAGACAAGTGTCTTAGTTTAAACATGTCGTCAAAAGAATGATCAAATAGTAAATTTTGGATCTTTATGCTAAGACAATATGAATTAAAGAATATCTTATATTAGAATATTTAAAATGCCAAGAATATTTAAAATCACAAAATTTAAATCAACTATATTTGGCATTAGAATTAGTAAAATTGAGCCTTTCATAGTAACAATTTAATTGATCAAATAGATAGTGTTACTAATATAGGAATAAAAAAGGAATTTTCAAAACAGTTTAGGCTCAAAATTGAGAATATATGATTTGAAATTAATTATTCTCAATTTAACTGATATATGATCTTTTTAAGATTTGACAAAATTTTGAAACCCACAAATCAAGAAATCAAGAATATATATAGTATAATAGTCAAGTTATTCTTGTTAGGACAAATACTTTAGAGGTTATCAATAAATCTCAAAATATATATTAATATAGAATATTTGATGCAAGAATATACAAAATTGAGTCTAAGAATTTTCACAATCATAACACTTTAAAAGAATATATTAAAAGTGTTATTACTATAAAAAAAATATATTTTCACATAGTATATTTTAGGCTCAAATATAGAGAATATACGATTTAAAAGTGTATATGATTCTCATATCAATTTACATCAAATATTTAGGAATTTCATAAGTAATTTCAATCCCAAATTTTAAGGGTGTAAAATAAATCAAATCTTCCAAATATTTTGGGTACAAGAGAAAATTATATTTTTATCCTAAAATAATATAATATTTACTAGTTAATTAGGGATGAAAAAATTCCTCACTGACACATTAATTAAGAAAATGAGAATTGTCTCAAAGTGTCGTAAAAGAGTAAAGCATCTTCGCGCAAAGTGAGAGAATATGATAATTTAAAAAGCAATTTATCCCCTCATAAAATCATGACAAATATGTACATAAGTCAAAAAATTCTAAAAAGGTTTTACCAAAATTTTAGAATAATAAATATTATTTATTTGGTAAAAATAAAATGAAATATGGAATTTTAAGGGTTAGTACCTCTCAGTATTGTAAAATCGAAAAAACATAAAAGTTAGATCTCTCATGAAATCGGCAACTTCTGATTGGGATTCCAAAGGTTTGATCGCCGAACCATAGAAAATTTGGAACACGAAAAAACAGTTGCTCACGACAACATGCTATTCATAAAACCCTTGAAACAATTATAGCATTCAGATTTGAGAATTAAAGCCATATATTAAGGGTTGAAAAGATACACAGCAACTTGCAAAACCAATAGTGCACACCAAGATTTGTGAAAGAAAATCGATAATGACACAAAACGGCAAAAAAGAAACACAATCGGTTTTAGTTGTGAAACAAGCACTATGGATTCAATAGGATGAGAATTTGAATCGGTATATTAAAAAAAATGTAGATTGATATTAACCATGATTCAATAGTATGAAAATTGATTGTTCATTCAAGATTACGATTCAACCATGATTTCAATAAAGTATGCAATTTTATATATATGATTACAATCATAGTCAAGTATGAATCATATGAATATGGTAAGTAACTAATCAATAATTCGATAACACAGAATAGCCACGATGATGGATTGCAAAAATATGGCAGAACAACCGATTCGATTTGTACATTTCATATTCAATTTATACAATATAATTGGCAAACAACAATAGCTATAACATGTATCTATTTGGCTCTGATACCAATTGATGAAAGTTTTAGTGTTTAATCATGGATCACTAGATACAAATTATAGCATATCATCAATAATTAAAAAAGAACAAGAGATTGTGTACCTTTGAATTAGGTTGATGAAGGATAACCATAGACAACGCCGACAACTATGAGAGGGGTAACTACCCTCATTCCGTCTAGCTCTAGGTTTTCTCACCAATCTTCAGAATAATTAACCGTCTAATGGGATGACGTTTAAATAGCTAAACGGCTGAGGGCAGGGACCTCTCATTGGGCTTATCTAAATGGTCCAACCCATCACGGTCCATTTAGTAACAAAAGCCCAATCACTCAAATTCATCATATAATTAGTGCTTAACACAATAGACCATAATTTATATTATGACATCAACTTATAATAATTGATTATTGTCAATCCATAATTGATTAATTAAATAATTAATCCAACATGAAATGGATATAGAAAGTGAGTTTACTTATTTCTCATGCTTTGTACCTTTTCTATCTGGGTTGAGATTGTATGTGAATGTGCAAAATGCAAAAATGAGAGTCTTATTTCTCAATAAATACAAAGTGGGTCTATCTATTATTATTATCTTCTGTTCTTGAATTTGTCTTTACCTATTTGAAGGTGATGGAGATGAGATTCATGCAGCATTAACTGAGTGGACAGGGCAAAGGACAGTACCTAATGTGTTTATTGGAGGCAAGCACATTGGTGGTTGTGACTGTAAGGCTACACACTCCTGTTGCTTCTTTTTTCATGTGGCAAAGACAAAATTAGAGTTGTTTTTTTCTTTCTCTGTTTTCATTATTTGTGTGTGTAATATTTTTATCACATGTTTATTTGTTGCAGCTGTTTTGGAGAAACACCGTGCAGGTCAATTGATTCCCCTTCTCACTGATGCTGGAGCGATCGCCAAGAACTCTGCTCAGCTCTGA

Protein sequence

>MS.gene25978.t1

MGSMLGSQKTQGTKEEMETTLNKTKQIAASSPVFVFSKTYCGYCKRVKDLLKQLGATYKVLEMMKIHAALTEWTGQRTVPNVFIGGKHIGGCDSVLEKHRAGQLIPLLTDAGAIAKNSAQL