AlfalfaGEDB | MS.gene07179

M. sativa cultivar XinJiangDaYe / MS.gene07179

KEGG: K03676
GO: GO:0003674 GO:0003824 GO:0008150 GO:0009055 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0019725 GO:0042592 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008
PFAM: PF00462.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07179.t1	XP_013469215.1	100	99	0	0	1	99	1	99	8.50E-49	202.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07179.t1	Q9M9Y9	74.2	97	25	0	1	97	1	97	2.6e-38	159.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07179.t1	A0A072VPA8	100.0	99	0	0	1	99	1	99	6.1e-49	202.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052011	MS.gene07179	-0.801703	7.95E-49	-1.69E-46
MS.gene054183	MS.gene07179	0.820012	8.59E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene035290	MS.gene07179	PPI
MS.gene23007	MS.gene07179	PPI
MS.gene07179	MS.gene022311	PPI
MS.gene70642	MS.gene07179	PPI
MS.gene20703	MS.gene07179	PPI
MS.gene07179	MS.gene07180	PPI
MS.gene07179	MS.gene20701	PPI
MS.gene07179	MS.gene07182	PPI
MS.gene07179	MS.gene03026	PPI
MS.gene25976	MS.gene07179	PPI
MS.gene07179	MS.gene036527	PPI
MS.gene07179	MS.gene29370	PPI
MS.gene07179	MS.gene006982	PPI
MS.gene23005	MS.gene07179	PPI
MS.gene036524	MS.gene07179	PPI
MS.gene07179	MS.gene050229	PPI
MS.gene07179	MS.gene52509	PPI
MS.gene022312	MS.gene07179	PPI
MS.gene07179	MS.gene041521	PPI
MS.gene07179	MS.gene07181	PPI
MS.gene07179	MS.gene23008	PPI
MS.gene07179	MS.gene03024	PPI
MS.gene07179	MS.gene005629	PPI
MS.gene07179	MS.gene07183	PPI
MS.gene07179	MS.gene03022	PPI
MS.gene005632	MS.gene07179	PPI
MS.gene58935	MS.gene07179	PPI
MS.gene07180	MS.gene07179	PPI
MS.gene07179	MS.gene20705	PPI
MS.gene07179	MS.gene23009	PPI
MS.gene07179	MS.gene041519	PPI
MS.gene07179	MS.gene005628	PPI
MS.gene07179	MS.gene005630	PPI
MS.gene07179	MS.gene035294	PPI
MS.gene07179	MS.gene59643	PPI
MS.gene29371	MS.gene07179	PPI
MS.gene03023	MS.gene07179	PPI
MS.gene07179	MS.gene035291	PPI
MS.gene07179	MS.gene20704	PPI
MS.gene006980	MS.gene07179	PPI
MS.gene07179	MS.gene58937	PPI
MS.gene07179	MS.gene87167	PPI
MS.gene005633	MS.gene07179	PPI
MS.gene07179	MS.gene005631	PPI
MS.gene07179	MS.gene29979	PPI
MS.gene07179	MS.gene25978	PPI
MS.gene07179	MS.gene041518	PPI
MS.gene036528	MS.gene07179	PPI
MS.gene07179	MS.gene20702	PPI
MS.gene07179	MS.gene70641	PPI
MS.gene20702	MS.gene07179	PPI
MS.gene006983	MS.gene07179	PPI
MS.gene07179	MS.gene006984	PPI
MS.gene07183	MS.gene07179	PPI
MS.gene07179	MS.gene58938	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07179.t1	MTR_1g088925	100.000	99	0	0	1	99	1	99	1.58e-69	202
MS.gene07179.t1	MTR_1g088920	96.970	99	3	0	1	99	1	99	1.58e-68	200
MS.gene07179.t1	MTR_7g108200	77.551	98	22	0	1	98	1	98	9.79e-58	173
MS.gene07179.t1	MTR_2g019900	56.842	95	41	0	1	95	1	95	7.47e-39	125
MS.gene07179.t1	MTR_1g088910	52.041	98	47	0	1	98	1	98	3.49e-36	118
MS.gene07179.t1	MTR_2g019950	54.737	95	43	0	1	95	1	95	7.51e-36	117
MS.gene07179.t1	MTR_7g022690	51.042	96	46	1	1	95	30	125	3.39e-34	114
MS.gene07179.t1	MTR_7g022710	50.000	96	47	1	1	95	30	125	1.12e-33	113
MS.gene07179.t1	MTR_7g022550	50.000	96	47	1	1	95	30	125	9.00e-33	110
MS.gene07179.t1	MTR_4g119050	50.000	96	47	1	1	95	1	96	4.99e-32	108
MS.gene07179.t1	MTR_4g119030	50.000	96	47	1	1	95	1	96	4.99e-32	108
MS.gene07179.t1	MTR_4g119040	50.000	96	47	1	1	95	1	96	4.99e-32	108
MS.gene07179.t1	MTR_7g026770	51.042	96	46	1	1	95	22	117	2.37e-31	112
MS.gene07179.t1	MTR_7g108220	49.474	95	47	1	1	94	1	95	8.12e-31	105
MS.gene07179.t1	MTR_7g108210	48.421	95	48	1	1	94	1	95	5.89e-30	103
MS.gene07179.t1	MTR_7g108250	41.053	95	56	0	1	95	1	95	1.78e-25	91.7
MS.gene07179.t1	MTR_1g088905	38.298	94	58	0	1	94	1	94	1.02e-24	89.7
MS.gene07179.t1	MTR_1g088895	42.857	98	56	0	1	98	1	98	3.39e-21	80.9
MS.gene07179.t1	MTR_2g048970	38.776	98	56	1	2	95	29	126	1.67e-20	79.7
MS.gene07179.t1	MTR_7g108260	40.426	94	56	0	1	94	1	94	6.77e-20	77.4
MS.gene07179.t1	MTR_2g014760	37.234	94	59	0	2	95	27	120	2.77e-19	76.6
MS.gene07179.t1	MTR_2g090755	37.500	96	53	2	4	95	41	133	1.01e-18	75.5
MS.gene07179.t1	MTR_4g079110	38.710	93	52	3	6	95	58	148	5.50e-16	68.9
MS.gene07179.t1	MTR_3g077570	34.737	95	62	0	1	95	18	112	6.27e-16	67.8
MS.gene07179.t1	MTR_3g077560	32.979	94	63	0	1	94	21	114	2.67e-15	66.2
MS.gene07179.t1	MTR_7g035245	36.170	94	60	0	1	94	3	96	5.25e-15	65.1
MS.gene07179.t1	MTR_1g069255	32.979	94	62	1	2	94	84	177	4.37e-14	64.7
MS.gene07179.t1	MTR_1g015890	36.170	94	58	1	3	94	48	141	6.19e-14	63.5
MS.gene07179.t1	MTR_3g104510	35.484	93	54	3	3	94	23	110	3.88e-13	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07179.t1	AT1G06830	74.227	97	25	0	1	97	1	97	2.30e-52	159
MS.gene07179.t1	AT2G30540	67.347	98	32	0	1	98	1	98	6.91e-52	158
MS.gene07179.t1	AT2G47880	71.429	98	28	0	1	98	1	98	7.97e-52	158
MS.gene07179.t1	AT3G62960	69.388	98	30	0	1	98	1	98	2.70e-50	154
MS.gene07179.t1	AT3G62950	50.505	99	48	1	1	98	1	99	4.64e-37	120
MS.gene07179.t1	AT2G47870	54.167	96	43	1	1	95	1	96	1.77e-36	119
MS.gene07179.t1	AT4G15670	50.526	95	47	0	1	95	1	95	1.91e-34	114
MS.gene07179.t1	AT4G15680	51.579	95	46	0	1	95	1	95	6.22e-34	113
MS.gene07179.t1	AT3G21460	53.684	95	44	0	1	95	1	95	7.33e-34	112
MS.gene07179.t1	AT4G15660	49.485	97	49	0	1	97	1	97	7.41e-34	112
MS.gene07179.t1	AT4G15700	51.579	95	46	0	1	95	1	95	7.49e-34	112
MS.gene07179.t1	AT4G15690	50.526	95	47	0	1	95	1	95	6.41e-33	110
MS.gene07179.t1	AT5G18600	46.316	95	51	0	1	95	1	95	4.73e-31	105
MS.gene07179.t1	AT5G14070	42.718	103	51	2	1	95	31	133	6.76e-26	94.0
MS.gene07179.t1	AT3G62930	41.053	95	56	0	1	95	1	95	3.75e-25	90.9
MS.gene07179.t1	AT3G02000	42.424	99	51	1	3	95	31	129	1.54e-24	90.5
MS.gene07179.t1	AT1G03020	43.158	95	54	0	1	95	1	95	1.89e-24	89.0
MS.gene07179.t1	AT1G28480	37.755	98	57	2	2	95	33	130	8.39e-18	73.2
MS.gene07179.t1	AT4G33040	40.206	97	52	2	3	94	41	136	9.22e-17	70.5
MS.gene07179.t1	AT5G40370	34.043	94	62	0	1	94	3	96	5.86e-15	65.1
MS.gene07179.t1	AT5G11930	39.785	93	46	3	3	94	57	140	1.56e-14	65.1
MS.gene07179.t1	AT4G28730	31.915	94	63	1	2	94	71	164	6.37e-14	63.9
MS.gene07179.t1	AT5G63030	36.585	82	52	0	13	94	31	112	2.14e-12	58.9
MS.gene07179.t1	AT2G20270	32.979	94	62	1	2	94	76	169	9.95e-12	58.2

Find 37 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTCCATTTCTTTCCCTTC+AGG	0.260417	1.4:-70671904	None:intergenic
CAGGGTACACTCCAATTTCT+TGG	0.312373	1.4:-70671858	None:intergenic
AGATCCTGAAGGGAAAGAAA+TGG	0.410754	1.4:+70671900	MS.gene07179:CDS
GGAGAAAGCTATAACAAAGT+TGG	0.418322	1.4:+70671921	MS.gene07179:CDS
CCTGTCCCTGCAGTATTCAT+TGG	0.431206	1.4:+70671955	MS.gene07179:CDS
GTGTAGGGACATTACTTCAT+TGG	0.438643	1.4:-70671998	None:intergenic
GAGAAAGCTATAACAAAGTT+GGG	0.448427	1.4:+70671922	MS.gene07179:CDS
AAATCAAAGTAAGCTAAATT+CGG	0.449656	1.4:-70672056	None:intergenic
GATGAGGTTGGCTACAGAAA+AGG	0.457049	1.4:+70671777	MS.gene07179:CDS
TTGTCAATTTCATGAATCAC+AGG	0.463222	1.4:-70671877	None:intergenic
CTTCCTCCAATGAATACTGC+AGG	0.488474	1.4:-70671961	None:intergenic
GCATAGACAACAAGAGCTCT+TGG	0.505172	1.4:-70671815	None:intergenic
TCATTGGAGGAAGGCTGGTG+GGG	0.511910	1.4:+70671971	MS.gene07179:CDS
TTCATTGGAGGAAGGCTGGT+GGG	0.512409	1.4:+70671970	MS.gene07179:CDS
ATGAGGTTGGCTACAGAAAA+GGG	0.514459	1.4:+70671778	MS.gene07179:CDS
GATGGAGAAGGTGATGAGGT+TGG	0.515048	1.4:+70671765	None:intergenic
CCAATGAATACTGCAGGGAC+AGG	0.522380	1.4:-70671955	None:intergenic
AGTATTCATTGGAGGAAGGC+TGG	0.536333	1.4:+70671966	MS.gene07179:CDS
GTCCCTGCAGTATTCATTGG+AGG	0.543693	1.4:+70671958	MS.gene07179:CDS
AAAGTAAGCTAAATTCGGTA+TGG	0.551896	1.4:-70672051	None:intergenic
TAGGGACATTACTTCATTGG+TGG	0.552920	1.4:-70671995	None:intergenic
ATTCGGTATGGCCTGAGTAG+TGG	0.556723	1.4:-70672039	None:intergenic
CGAGTGAACCACTTAGGTGT+AGG	0.564629	1.4:-70672014	None:intergenic
ATTCATTGGAGGAAGGCTGG+TGG	0.573512	1.4:+70671969	MS.gene07179:CDS
GTTCACTCGTTCCACTACTC+AGG	0.574323	1.4:+70672028	MS.gene07179:CDS
GAAATTGACAAAGATCCTGA+AGG	0.576983	1.4:+70671889	MS.gene07179:CDS
GAGTGAACCACTTAGGTGTA+GGG	0.588505	1.4:-70672013	None:intergenic
GGCTACAGAAAAGGGTGTGG+TGG	0.590705	1.4:+70671786	MS.gene07179:CDS
TTATAATATTAAGATGGAGA+AGG	0.591892	1.4:+70671753	None:intergenic
GTGGAACGAGTGAACCACTT+AGG	0.595515	1.4:-70672020	None:intergenic
CTGCAGTATTCATTGGAGGA+AGG	0.598971	1.4:+70671962	MS.gene07179:CDS
GTAATGTCCCTACACCTAAG+TGG	0.609456	1.4:+70672006	MS.gene07179:CDS
AAATTGACAAAGATCCTGAA+GGG	0.633441	1.4:+70671890	MS.gene07179:CDS
TTCCTCCAATGAATACTGCA+GGG	0.637919	1.4:-70671960	None:intergenic
TGTCAATTTCATGAATCACA+GGG	0.646979	1.4:-70671876	None:intergenic
GTTGGCTACAGAAAAGGGTG+TGG	0.660529	1.4:+70671783	MS.gene07179:CDS
TTAAGATGGAGAAGGTGATG+AGG	0.668317	1.4:+70671761	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACATTTTGTTCCAAGAAAT+TGG	+	chr1.4:70671847-70671866	MS.gene07179:CDS	25.0%
	AAATTGACAAAGATCCTGAA+GGG	+	chr1.4:70671890-70671909	MS.gene07179:CDS	30.0%
	GAGAAAGCTATAACAAAGTT+GGG	+	chr1.4:70671922-70671941	MS.gene07179:CDS	30.0%
	TGTCAATTTCATGAATCACA+GGG	-	chr1.4:70671879-70671898	None:intergenic	30.0%
	TTGTCAATTTCATGAATCAC+AGG	-	chr1.4:70671880-70671899	None:intergenic	30.0%
	GAAATTGACAAAGATCCTGA+AGG	+	chr1.4:70671889-70671908	MS.gene07179:CDS	35.0%
	GGAGAAAGCTATAACAAAGT+TGG	+	chr1.4:70671921-70671940	MS.gene07179:CDS	35.0%
	AGATCCTGAAGGGAAAGAAA+TGG	+	chr1.4:70671900-70671919	MS.gene07179:CDS	40.0%
	GTGTAGGGACATTACTTCAT+TGG	-	chr1.4:70672001-70672020	None:intergenic	40.0%
	TAGGGACATTACTTCATTGG+TGG	-	chr1.4:70671998-70672017	None:intergenic	40.0%
	TTCCTCCAATGAATACTGCA+GGG	-	chr1.4:70671963-70671982	None:intergenic	40.0%
	TTCTCCATTTCTTTCCCTTC+AGG	-	chr1.4:70671907-70671926	None:intergenic	40.0%
!	ATGAGGTTGGCTACAGAAAA+GGG	+	chr1.4:70671778-70671797	MS.gene07179:CDS	40.0%
	AGTATTCATTGGAGGAAGGC+TGG	+	chr1.4:70671966-70671985	MS.gene07179:CDS	45.0%
	CAGGGTACACTCCAATTTCT+TGG	-	chr1.4:70671861-70671880	None:intergenic	45.0%
	CTGCAGTATTCATTGGAGGA+AGG	+	chr1.4:70671962-70671981	MS.gene07179:CDS	45.0%
	CTTCCTCCAATGAATACTGC+AGG	-	chr1.4:70671964-70671983	None:intergenic	45.0%
	GAGTGAACCACTTAGGTGTA+GGG	-	chr1.4:70672016-70672035	None:intergenic	45.0%
	GCATAGACAACAAGAGCTCT+TGG	-	chr1.4:70671818-70671837	None:intergenic	45.0%
	GTAATGTCCCTACACCTAAG+TGG	+	chr1.4:70672006-70672025	MS.gene07179:CDS	45.0%
!	GATGAGGTTGGCTACAGAAA+AGG	+	chr1.4:70671777-70671796	MS.gene07179:CDS	45.0%
	ATTCATTGGAGGAAGGCTGG+TGG	+	chr1.4:70671969-70671988	MS.gene07179:CDS	50.0%
	ATTCGGTATGGCCTGAGTAG+TGG	-	chr1.4:70672042-70672061	None:intergenic	50.0%
	CCAATGAATACTGCAGGGAC+AGG	-	chr1.4:70671958-70671977	None:intergenic	50.0%
	CCTGTCCCTGCAGTATTCAT+TGG	+	chr1.4:70671955-70671974	MS.gene07179:CDS	50.0%
	CGAGTGAACCACTTAGGTGT+AGG	-	chr1.4:70672017-70672036	None:intergenic	50.0%
	GTCCCTGCAGTATTCATTGG+AGG	+	chr1.4:70671958-70671977	MS.gene07179:CDS	50.0%
	GTGGAACGAGTGAACCACTT+AGG	-	chr1.4:70672023-70672042	None:intergenic	50.0%
	GTTCACTCGTTCCACTACTC+AGG	+	chr1.4:70672028-70672047	MS.gene07179:CDS	50.0%
	GTTGGCTACAGAAAAGGGTG+TGG	+	chr1.4:70671783-70671802	MS.gene07179:CDS	50.0%
	TTCATTGGAGGAAGGCTGGT+GGG	+	chr1.4:70671970-70671989	MS.gene07179:CDS	50.0%
	TCATTGGAGGAAGGCTGGTG+GGG	+	chr1.4:70671971-70671990	MS.gene07179:CDS	55.0%
!	GGCTACAGAAAAGGGTGTGG+TGG	+	chr1.4:70671786-70671805	MS.gene07179:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	70671766	70672065	70671766	ID=MS.gene07179
chr1.4	mRNA	70671766	70672065	70671766	ID=MS.gene07179.t1;Parent=MS.gene07179
chr1.4	exon	70671766	70672065	70671766	ID=MS.gene07179.t1.exon1;Parent=MS.gene07179.t1
chr1.4	CDS	70671766	70672065	70671766	ID=cds.MS.gene07179.t1;Parent=MS.gene07179.t1

Gene Sequence	>MS.gene07179 ATGGAGAAGGTGATGAGGTTGGCTACAGAAAAGGGTGTGGTGGTATTCACCAAGAGCTCTTGTTGTCTATGCTATGCAGTGAACATTTTGTTCCAAGAAATTGGAGTGTACCCTGTGATTCATGAAATTGACAAAGATCCTGAAGGGAAAGAAATGGAGAAAGCTATAACAAAGTTGGGTTGTAATGCACCTGTCCCTGCAGTATTCATTGGAGGAAGGCTGGTGGGGTCCACCAATGAAGTAATGTCCCTACACCTAAGTGGTTCACTCGTTCCACTACTCAGGCCATACCGAATTTAG
Protein sequence	>MS.gene07179.t1 MEKVMRLATEKGVVVFTKSSCCLCYAVNILFQEIGVYPVIHEIDKDPEGKEMEKAITKLGCNAPVPAVFIGGRLVGSTNEVMSLHLSGSLVPLLRPYRI