AlfalfaGEDB | MS.gene23005

M. sativa cultivar XinJiangDaYe / MS.gene23005

KEGG: K03676
GO: GO:0003674 GO:0003824 GO:0008150 GO:0009055 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0019725 GO:0042592 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008
PFAM: PF00462.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23005.t1	XP_003625874.1	99	102	1	0	1	102	1	102	1.80E-49	204.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23005.t1	Q9LYC8	63.7	102	37	0	1	102	1	102	3.4e-33	142.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23005.t1	B7FMW2	99.0	102	1	0	1	102	1	102	1.3e-49	204.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene23005	MS.gene035290	PPI
MS.gene23005	MS.gene59643	PPI
MS.gene23005	MS.gene035291	PPI
MS.gene23005	MS.gene20704	PPI
MS.gene23005	MS.gene58938	PPI
MS.gene23005	MS.gene87167	PPI
MS.gene23005	MS.gene005631	PPI
MS.gene23005	MS.gene29979	PPI
MS.gene23005	MS.gene022311	PPI
MS.gene23005	MS.gene005633	PPI
MS.gene23005	MS.gene006980	PPI
MS.gene23005	MS.gene041518	PPI
MS.gene23005	MS.gene022312	PPI
MS.gene23005	MS.gene20702	PPI
MS.gene23005	MS.gene70641	PPI
MS.gene23005	MS.gene29371	PPI
MS.gene23005	MS.gene006984	PPI
MS.gene23005	MS.gene07179	PPI
MS.gene23005	MS.gene20701	PPI
MS.gene23005	MS.gene006983	PPI
MS.gene23005	MS.gene07182	PPI
MS.gene23005	MS.gene03026	PPI
MS.gene23005	MS.gene58935	PPI
MS.gene23005	MS.gene050229	PPI
MS.gene23005	MS.gene041521	PPI
MS.gene23005	MS.gene07181	PPI
MS.gene23005	MS.gene70642	PPI
MS.gene23005	MS.gene23008	PPI
MS.gene23005	MS.gene07180	PPI
MS.gene23005	MS.gene23007	PPI
MS.gene23005	MS.gene20705	PPI
MS.gene23005	MS.gene036527	PPI
MS.gene23005	MS.gene29370	PPI
MS.gene23005	MS.gene006982	PPI
MS.gene23005	MS.gene005628	PPI
MS.gene23005	MS.gene036528	PPI
MS.gene23007	MS.gene23005	PPI
MS.gene23005	MS.gene20703	PPI
MS.gene23005	MS.gene035294	PPI
MS.gene23005	MS.gene52509	PPI
MS.gene23005	MS.gene03024	PPI
MS.gene23005	MS.gene005629	PPI
MS.gene23005	MS.gene07183	PPI
MS.gene23005	MS.gene03022	PPI
MS.gene005632	MS.gene23005	PPI
MS.gene23005	MS.gene58937	PPI
MS.gene23005	MS.gene03023	PPI
MS.gene23005	MS.gene23009	PPI
MS.gene23005	MS.gene25976	PPI
MS.gene23005	MS.gene005632	PPI
MS.gene23005	MS.gene041519	PPI
MS.gene23005	MS.gene005630	PPI
MS.gene23005	MS.gene25978	PPI
MS.gene23005	MS.gene036524	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23005.t1	MTR_7g108250	99.020	102	1	0	1	102	1	102	6.73e-72	209
MS.gene23005.t1	MTR_1g088905	75.490	102	25	0	1	102	1	102	4.57e-56	169
MS.gene23005.t1	MTR_1g088895	58.824	102	42	0	1	102	1	102	6.57e-40	128
MS.gene23005.t1	MTR_2g019900	52.941	102	48	0	1	102	1	102	2.84e-39	126
MS.gene23005.t1	MTR_4g119050	55.340	103	45	1	1	102	1	103	3.81e-39	126
MS.gene23005.t1	MTR_4g119030	55.340	103	45	1	1	102	1	103	3.81e-39	126
MS.gene23005.t1	MTR_4g119040	55.340	103	45	1	1	102	1	103	3.81e-39	126
MS.gene23005.t1	MTR_7g108260	56.436	101	44	0	1	101	1	101	1.98e-36	119
MS.gene23005.t1	MTR_2g019950	46.078	102	55	0	1	102	1	102	6.27e-32	108
MS.gene23005.t1	MTR_1g088910	49.020	102	52	0	1	102	1	102	5.16e-30	103
MS.gene23005.t1	MTR_7g108220	48.544	103	52	1	1	102	1	103	9.12e-29	100
MS.gene23005.t1	MTR_7g108210	47.573	103	53	1	1	102	1	103	5.25e-28	98.2
MS.gene23005.t1	MTR_7g108200	45.263	95	52	0	1	95	1	95	6.19e-26	92.8
MS.gene23005.t1	MTR_1g088920	43.158	95	54	0	1	95	1	95	2.57e-25	91.3
MS.gene23005.t1	MTR_1g088925	41.053	95	56	0	1	95	1	95	3.94e-25	90.9
MS.gene23005.t1	MTR_7g022690	37.864	103	63	1	1	102	30	132	3.01e-23	87.0
MS.gene23005.t1	MTR_7g022550	37.864	103	63	1	1	102	30	132	4.71e-23	86.7
MS.gene23005.t1	MTR_7g022710	35.922	103	65	1	1	102	30	132	8.75e-23	85.9
MS.gene23005.t1	MTR_7g026770	39.806	103	61	1	1	102	22	124	2.69e-22	88.2
MS.gene23005.t1	MTR_2g014760	40.594	101	56	2	4	102	29	127	7.29e-22	83.2
MS.gene23005.t1	MTR_2g048970	40.952	105	58	1	2	102	29	133	1.23e-21	82.8
MS.gene23005.t1	MTR_4g079110	41.837	98	56	1	6	102	58	155	2.04e-20	80.5
MS.gene23005.t1	MTR_2g090755	35.922	103	59	3	4	102	41	140	5.61e-20	79.0
MS.gene23005.t1	MTR_3g077570	37.000	100	63	0	1	100	18	117	3.80e-18	73.6
MS.gene23005.t1	MTR_3g077560	35.000	100	65	0	1	100	21	120	1.86e-16	69.3
MS.gene23005.t1	MTR_1g015890	36.634	101	62	1	4	102	49	149	2.37e-16	69.7
MS.gene23005.t1	MTR_5g077550	36.047	86	53	1	19	102	38	123	1.49e-15	67.0
MS.gene23005.t1	MTR_7g035245	39.362	94	57	0	7	100	9	102	1.56e-14	63.9
MS.gene23005.t1	MTR_1g069255	36.364	99	62	1	2	99	84	182	2.44e-14	65.5
MS.gene23005.t1	MTR_3g104510	33.333	99	60	3	4	101	24	117	5.32e-13	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23005.t1	AT3G62930	63.725	102	37	0	1	102	1	102	6.51e-47	145
MS.gene23005.t1	AT1G03020	57.843	102	43	0	1	102	1	102	3.19e-44	139
MS.gene23005.t1	AT5G18600	56.863	102	44	0	1	102	1	102	1.63e-43	137
MS.gene23005.t1	AT4G15680	53.922	102	47	0	1	102	1	102	4.99e-40	128
MS.gene23005.t1	AT4G15690	53.922	102	47	0	1	102	1	102	7.57e-40	128
MS.gene23005.t1	AT4G15700	52.941	102	48	0	1	102	1	102	1.40e-39	127
MS.gene23005.t1	AT4G15670	50.000	102	51	0	1	102	1	102	1.12e-37	122
MS.gene23005.t1	AT4G15660	50.000	102	51	0	1	102	1	102	1.61e-37	122
MS.gene23005.t1	AT3G21460	44.118	102	57	0	1	102	1	102	5.31e-31	105
MS.gene23005.t1	AT3G62950	43.689	103	57	1	1	102	1	103	3.75e-28	98.6
MS.gene23005.t1	AT2G47870	42.718	103	58	1	1	102	1	103	1.79e-27	97.1
MS.gene23005.t1	AT1G06830	46.316	95	51	0	1	95	1	95	5.68e-27	95.5
MS.gene23005.t1	AT2G30540	45.263	95	52	0	1	95	1	95	2.01e-26	94.4
MS.gene23005.t1	AT2G47880	42.574	101	58	0	1	101	1	101	9.50e-26	92.4
MS.gene23005.t1	AT5G14070	42.056	107	54	2	4	102	34	140	1.03e-25	93.6
MS.gene23005.t1	AT3G62960	42.105	95	55	0	1	95	1	95	8.55e-24	87.4
MS.gene23005.t1	AT3G02000	39.048	105	58	1	4	102	32	136	1.99e-21	82.4
MS.gene23005.t1	AT1G28480	33.019	106	65	2	2	102	33	137	2.84e-18	74.3
MS.gene23005.t1	AT4G33040	36.893	103	61	1	4	102	42	144	1.08e-16	70.5
MS.gene23005.t1	AT5G63030	36.000	100	64	0	1	100	19	118	3.00e-16	68.9
MS.gene23005.t1	AT5G40370	39.000	100	61	0	1	100	3	102	4.42e-16	68.2
MS.gene23005.t1	AT2G20270	37.374	99	61	1	2	99	76	174	4.84e-16	69.7
MS.gene23005.t1	AT1G03850	34.694	98	56	2	7	102	59	150	2.30e-15	67.4
MS.gene23005.t1	AT5G11930	32.292	96	55	3	7	101	61	147	2.01e-14	64.7
MS.gene23005.t1	AT5G40370	40.698	86	51	0	15	100	42	127	1.39e-12	59.7

Find 24 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTATCAAGCTCATAAACAAT+TGG	0.370369	7.2:+6725479	None:intergenic
GAGCTTGATAAAATCGTAAA+CGG	0.380521	7.2:-6725467	MS.gene23005:CDS
TGAAAGTGAGTTGCTTCAAA+TGG	0.389664	7.2:-6725435	MS.gene23005:CDS
TATCAAGCTCATAAACAATT+GGG	0.416819	7.2:+6725480	None:intergenic
TGAAGGATGCTGGAGCTATA+TGG	0.425149	7.2:-6725310	MS.gene23005:CDS
GCTCCAGCATCCTTCAACAT+AGG	0.451145	7.2:+6725317	None:intergenic
ACATCACTGATACGTAGCTT+TGG	0.479409	7.2:-6725509	MS.gene23005:CDS
GAAAGTGAGTTGCTTCAAAT+GGG	0.482885	7.2:-6725434	MS.gene23005:CDS
GTACCTATGTTGAAGGATGC+TGG	0.486318	7.2:-6725320	MS.gene23005:CDS
AGCTTGATAAAATCGTAAAC+GGG	0.516191	7.2:-6725466	MS.gene23005:CDS
TCAATTAGTACCTATGTTGA+AGG	0.516443	7.2:-6725327	MS.gene23005:CDS
ATGAAAACAGCAGGTACACT+TGG	0.531554	7.2:+6725404	None:intergenic
CTCTTGCTGAATATCACCAC+TGG	0.562622	7.2:+6725557	None:intergenic
GACACAGTCACAAGCATGGT+TGG	0.566247	7.2:-6725587	MS.gene23005:CDS
TGTTGTCCTATGAAAACAGC+AGG	0.566301	7.2:+6725395	None:intergenic
CTAATTGATTCCTCACATGA+AGG	0.570406	7.2:+6725342	None:intergenic
AAGCTCATAAACAATTGGGT+TGG	0.585004	7.2:+6725484	None:intergenic
TTCATAGGACAACAATTCAG+AGG	0.593248	7.2:-6725386	MS.gene23005:CDS
ATAGGACAACAATTCAGAGG+TGG	0.626085	7.2:-6725383	MS.gene23005:CDS
AAATCATGAGCCTTCATGTG+AGG	0.628762	7.2:-6725352	MS.gene23005:CDS
CATGGACACAGTCACAAGCA+TGG	0.652752	7.2:-6725591	MS.gene23005:CDS
AAAGTGAGTTGCTTCAAATG+GGG	0.727772	7.2:-6725433	MS.gene23005:CDS
GTATCAGTGATGTTACTGAG+TGG	0.733718	7.2:+6725519	None:intergenic
CATGGTTGGTGAAAAGCCAG+TGG	0.752701	7.2:-6725573	MS.gene23005:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TATCAAGCTCATAAACAATT+GGG	+	chr7.2:6725417-6725436	None:intergenic	25.0%
!	TTATCAAGCTCATAAACAAT+TGG	+	chr7.2:6725418-6725437	None:intergenic	25.0%
	AGCTTGATAAAATCGTAAAC+GGG	-	chr7.2:6725428-6725447	MS.gene23005:CDS	30.0%
	GAGCTTGATAAAATCGTAAA+CGG	-	chr7.2:6725427-6725446	MS.gene23005:CDS	30.0%
	TCAATTAGTACCTATGTTGA+AGG	-	chr7.2:6725567-6725586	MS.gene23005:CDS	30.0%
!!!	ATGAAGGCTCATGATTTTTT+TGG	+	chr7.2:6725539-6725558	None:intergenic	30.0%
	AAAGTGAGTTGCTTCAAATG+GGG	-	chr7.2:6725461-6725480	MS.gene23005:CDS	35.0%
	AAGCTCATAAACAATTGGGT+TGG	+	chr7.2:6725413-6725432	None:intergenic	35.0%
	GAAAGTGAGTTGCTTCAAAT+GGG	-	chr7.2:6725460-6725479	MS.gene23005:CDS	35.0%
	TGAAAGTGAGTTGCTTCAAA+TGG	-	chr7.2:6725459-6725478	MS.gene23005:CDS	35.0%
	TTCATAGGACAACAATTCAG+AGG	-	chr7.2:6725508-6725527	MS.gene23005:CDS	35.0%
!	CTAATTGATTCCTCACATGA+AGG	+	chr7.2:6725555-6725574	None:intergenic	35.0%
	AAATCATGAGCCTTCATGTG+AGG	-	chr7.2:6725542-6725561	MS.gene23005:CDS	40.0%
	ACATCACTGATACGTAGCTT+TGG	-	chr7.2:6725385-6725404	MS.gene23005:CDS	40.0%
	ATAGGACAACAATTCAGAGG+TGG	-	chr7.2:6725511-6725530	MS.gene23005:CDS	40.0%
	ATGAAAACAGCAGGTACACT+TGG	+	chr7.2:6725493-6725512	None:intergenic	40.0%
	GTATCAGTGATGTTACTGAG+TGG	+	chr7.2:6725378-6725397	None:intergenic	40.0%
	TGTTGTCCTATGAAAACAGC+AGG	+	chr7.2:6725502-6725521	None:intergenic	40.0%
!!!	AGTGTACCTGCTGTTTTCAT+AGG	-	chr7.2:6725493-6725512	MS.gene23005:CDS	40.0%
	CTCTTGCTGAATATCACCAC+TGG	+	chr7.2:6725340-6725359	None:intergenic	45.0%
	GTACCTATGTTGAAGGATGC+TGG	-	chr7.2:6725574-6725593	MS.gene23005:CDS	45.0%
	TGAAGGATGCTGGAGCTATA+TGG	-	chr7.2:6725584-6725603	MS.gene23005:CDS	45.0%
	GACACAGTCACAAGCATGGT+TGG	-	chr7.2:6725307-6725326	MS.gene23005:CDS	50.0%
	GCTCCAGCATCCTTCAACAT+AGG	+	chr7.2:6725580-6725599	None:intergenic	50.0%
!!	CATGGTTGGTGAAAAGCCAG+TGG	-	chr7.2:6725321-6725340	MS.gene23005:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	6725304	6725612	6725304	ID=MS.gene23005
chr7.2	mRNA	6725304	6725612	6725304	ID=MS.gene23005.t1;Parent=MS.gene23005
chr7.2	exon	6725304	6725612	6725304	ID=MS.gene23005.t1.exon1;Parent=MS.gene23005.t1
chr7.2	CDS	6725304	6725612	6725304	ID=cds.MS.gene23005.t1;Parent=MS.gene23005.t1

Gene Sequence	>MS.gene23005 ATGGACACAGTCACAAGCATGGTTGGTGAAAAGCCAGTGGTGATATTCAGCAAGAGCACGTGTTGCTTGAGCCACTCAGTAACATCACTGATACGTAGCTTTGGAGCCAACCCAATTGTTTATGAGCTTGATAAAATCGTAAACGGGTCACAGATTGAAAGTGAGTTGCTTCAAATGGGGTGCAAACCAAGTGTACCTGCTGTTTTCATAGGACAACAATTCAGAGGTGGTTCCAAAAAAATCATGAGCCTTCATGTGAGGAATCAATTAGTACCTATGTTGAAGGATGCTGGAGCTATATGGATTTGA
Protein sequence	>MS.gene23005.t1 MDTVTSMVGEKPVVIFSKSTCCLSHSVTSLIRSFGANPIVYELDKIVNGSQIESELLQMGCKPSVPAVFIGQQFRGGSKKIMSLHVRNQLVPMLKDAGAIWI