AlfalfaGEDB | MS.gene20705

M. sativa cultivar XinJiangDaYe / MS.gene20705

KEGG: K03676
GO: GO:0003674 GO:0003824 GO:0008150 GO:0009055 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0019725 GO:0042592 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008
PFAM: PF00462.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20705.t1	PNX99618.1	100	102	0	0	1	102	52	153	1.20E-50	208.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20705.t1	Q9LYC6	75.5	102	24	1	1	101	1	102	8.3e-40	164.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20705.t1	B7FMA1	100.0	102	0	0	1	102	1	102	8.8e-51	208.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene20705	MS.gene03022	PPI
MS.gene07182	MS.gene20705	PPI
MS.gene52509	MS.gene20705	PPI
MS.gene20705	MS.gene23009	PPI
MS.gene005633	MS.gene20705	PPI
MS.gene20705	MS.gene005628	PPI
MS.gene29979	MS.gene20705	PPI
MS.gene20705	MS.gene005630	PPI
MS.gene036528	MS.gene20705	PPI
MS.gene20705	MS.gene035294	PPI
MS.gene20702	MS.gene20705	PPI
MS.gene20705	MS.gene59643	PPI
MS.gene006983	MS.gene20705	PPI
MS.gene20705	MS.gene20704	PPI
MS.gene20705	MS.gene005629	PPI
MS.gene20705	MS.gene58937	PPI
MS.gene07183	MS.gene20705	PPI
MS.gene035290	MS.gene20705	PPI
MS.gene87167	MS.gene20705	PPI
MS.gene23007	MS.gene20705	PPI
MS.gene20705	MS.gene005631	PPI
MS.gene70642	MS.gene20705	PPI
MS.gene20703	MS.gene20705	PPI
MS.gene20705	MS.gene006984	PPI
MS.gene07179	MS.gene20705	PPI
MS.gene25976	MS.gene20705	PPI
MS.gene20705	MS.gene58938	PPI
MS.gene041521	MS.gene20705	PPI
MS.gene23005	MS.gene20705	PPI
MS.gene036524	MS.gene20705	PPI
MS.gene20705	MS.gene022311	PPI
MS.gene022312	MS.gene20705	PPI
MS.gene70641	MS.gene20705	PPI
MS.gene005632	MS.gene20705	PPI
MS.gene20705	MS.gene20701	PPI
MS.gene58935	MS.gene20705	PPI
MS.gene07180	MS.gene20705	PPI
MS.gene23009	MS.gene20705	PPI
MS.gene20705	MS.gene036527	PPI
MS.gene20705	MS.gene29370	PPI
MS.gene20705	MS.gene006982	PPI
MS.gene041519	MS.gene20705	PPI
MS.gene035291	MS.gene20705	PPI
MS.gene20705	MS.gene050229	PPI
MS.gene29371	MS.gene20705	PPI
MS.gene03023	MS.gene20705	PPI
MS.gene03026	MS.gene20705	PPI
MS.gene25978	MS.gene20705	PPI
MS.gene20705	MS.gene07181	PPI
MS.gene20705	MS.gene23008	PPI
MS.gene20705	MS.gene03024	PPI
MS.gene006980	MS.gene20705	PPI
MS.gene041518	MS.gene20705	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20705.t1	MTR_1g088910	100.000	102	0	0	1	102	1	102	6.54e-74	214
MS.gene20705.t1	MTR_7g108220	89.320	103	10	1	1	102	1	103	1.42e-63	187
MS.gene20705.t1	MTR_7g108210	88.350	103	11	1	1	102	1	103	6.74e-63	186
MS.gene20705.t1	MTR_2g019950	63.725	102	37	0	1	102	1	102	8.83e-45	140
MS.gene20705.t1	MTR_2g019900	58.416	101	42	0	1	101	1	101	4.46e-39	126
MS.gene20705.t1	MTR_7g108200	57.447	94	40	0	1	94	1	94	1.79e-37	122
MS.gene20705.t1	MTR_1g088920	54.082	98	45	0	1	98	1	98	1.74e-36	119
MS.gene20705.t1	MTR_1g088925	52.041	98	47	0	1	98	1	98	3.60e-36	118
MS.gene20705.t1	MTR_4g119050	53.398	103	47	1	1	102	1	103	3.11e-33	111
MS.gene20705.t1	MTR_4g119030	53.398	103	47	1	1	102	1	103	3.11e-33	111
MS.gene20705.t1	MTR_4g119040	53.398	103	47	1	1	102	1	103	3.11e-33	111
MS.gene20705.t1	MTR_7g026770	51.456	103	49	1	1	102	22	124	5.95e-32	113
MS.gene20705.t1	MTR_7g108250	49.020	102	52	0	1	102	1	102	2.62e-30	103
MS.gene20705.t1	MTR_1g088905	49.020	102	52	0	1	102	1	102	5.10e-30	103
MS.gene20705.t1	MTR_7g022550	47.573	103	53	1	1	102	30	132	7.81e-30	103
MS.gene20705.t1	MTR_7g022690	47.573	103	53	1	1	102	30	132	9.00e-30	103
MS.gene20705.t1	MTR_7g022710	47.573	103	53	1	1	102	30	132	2.80e-29	102
MS.gene20705.t1	MTR_7g108260	45.098	102	56	0	1	102	1	102	7.09e-29	100
MS.gene20705.t1	MTR_1g088895	48.485	99	51	0	4	102	4	102	2.74e-25	91.7
MS.gene20705.t1	MTR_2g048970	39.048	105	60	1	2	102	29	133	5.99e-18	73.6
MS.gene20705.t1	MTR_3g077570	37.000	100	63	0	1	100	18	117	8.17e-17	70.1
MS.gene20705.t1	MTR_7g035245	35.000	100	65	0	1	100	3	102	6.95e-16	67.4
MS.gene20705.t1	MTR_1g069255	34.343	99	64	1	2	99	84	182	1.71e-15	68.6
MS.gene20705.t1	MTR_2g090755	32.673	101	61	2	6	102	43	140	2.04e-15	67.0
MS.gene20705.t1	MTR_3g077560	34.000	100	66	0	1	100	21	120	1.46e-14	64.7
MS.gene20705.t1	MTR_3g104510	35.000	100	59	2	3	101	23	117	2.51e-14	63.9
MS.gene20705.t1	MTR_4g079110	33.333	99	63	2	6	102	58	155	5.98e-14	63.5
MS.gene20705.t1	MTR_1g015890	37.255	102	60	2	3	101	48	148	1.03e-13	63.2
MS.gene20705.t1	MTR_2g014760	30.000	100	70	0	3	102	28	127	1.16e-13	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20705.t1	AT3G62950	74.757	103	25	1	1	102	1	103	1.02e-55	168
MS.gene20705.t1	AT2G47870	74.757	103	25	1	1	102	1	103	2.51e-53	162
MS.gene20705.t1	AT3G21460	55.882	102	45	0	1	102	1	102	1.21e-39	127
MS.gene20705.t1	AT2G47880	49.020	102	52	0	1	102	1	102	1.67e-34	114
MS.gene20705.t1	AT4G15690	48.039	102	53	0	1	102	1	102	2.90e-33	111
MS.gene20705.t1	AT5G18600	50.495	101	50	0	1	101	1	101	3.10e-33	111
MS.gene20705.t1	AT4G15670	47.059	102	54	0	1	102	1	102	5.24e-33	110
MS.gene20705.t1	AT4G15700	48.039	102	53	0	1	102	1	102	5.66e-33	110
MS.gene20705.t1	AT4G15680	47.059	102	54	0	1	102	1	102	6.46e-33	110
MS.gene20705.t1	AT3G62960	52.128	94	45	0	1	94	1	94	1.18e-32	110
MS.gene20705.t1	AT2G30540	48.485	99	51	0	1	99	1	99	1.08e-31	107
MS.gene20705.t1	AT4G15660	46.078	102	55	0	1	102	1	102	1.20e-31	107
MS.gene20705.t1	AT1G06830	52.222	90	43	0	1	90	1	90	3.79e-31	105
MS.gene20705.t1	AT3G62930	49.505	101	51	0	1	101	1	101	1.43e-29	102
MS.gene20705.t1	AT5G14070	46.364	110	51	2	1	102	31	140	2.61e-29	102
MS.gene20705.t1	AT1G03020	47.059	102	54	0	1	102	1	102	4.79e-28	98.2
MS.gene20705.t1	AT3G02000	47.170	106	50	1	3	102	31	136	1.04e-25	93.6
MS.gene20705.t1	AT5G40370	34.000	100	66	0	1	100	3	102	9.75e-16	67.4
MS.gene20705.t1	AT1G28480	30.476	105	69	2	2	102	33	137	4.02e-15	66.2
MS.gene20705.t1	AT4G28730	32.323	99	66	1	2	99	71	169	2.29e-14	65.1
MS.gene20705.t1	AT5G63030	34.000	100	66	0	1	100	19	118	5.89e-14	63.2
MS.gene20705.t1	AT4G33040	33.333	105	64	2	3	102	41	144	2.40e-13	62.0
MS.gene20705.t1	AT5G20500	37.634	93	58	0	2	94	33	125	5.12e-13	60.8
MS.gene20705.t1	AT2G20270	31.313	99	67	1	2	99	76	174	5.61e-12	58.9
MS.gene20705.t1	AT5G40370	36.047	86	55	0	15	100	42	127	3.33e-11	56.2
MS.gene20705.t1	AT5G11930	33.000	100	57	3	3	101	57	147	3.40e-11	56.6

Find 26 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATGGCTGCTAGGGCCATT+TGG	0.373171	1.2:-66572783	MS.gene20705:CDS
TTCATTGGTGGAAAATTTGT+AGG	0.394277	1.2:-66572859	MS.gene20705:CDS
TCAGTGCCAGCAGTGTTCAT+TGG	0.405054	1.2:-66572874	MS.gene20705:CDS
GTGATATCTCTTCATGTTGA+TGG	0.409792	1.2:-66572823	MS.gene20705:CDS
GAAAGAGCTTTGAGGAGTCT+TGG	0.445088	1.2:-66572907	MS.gene20705:CDS
TGAACCCTATGGTAGGGAAA+TGG	0.445205	1.2:-66572929	MS.gene20705:CDS
AATGGATAGAGTGAAGGATT+TGG	0.446072	1.2:-66573064	MS.gene20705:CDS
ATGAACACTGCTGGCACTGA+AGG	0.449282	1.2:+66572877	None:intergenic
CTTTCCATTTCCCTACCATA+GGG	0.473255	1.2:+66572925	None:intergenic
TTGTCAAGCTCATGCACAGC+AGG	0.496748	1.2:+66572952	None:intergenic
TCTTTCCATTTCCCTACCAT+AGG	0.504914	1.2:+66572924	None:intergenic
ACAAATGCTCATGGCTGCTA+GGG	0.507037	1.2:-66572791	MS.gene20705:CDS
AACAAATGCTCATGGCTGCT+AGG	0.510969	1.2:-66572792	MS.gene20705:CDS
TTTCCACCAATGAACACTGC+TGG	0.522815	1.2:+66572868	None:intergenic
GGGAAATGGAAAGAGCTTTG+AGG	0.524086	1.2:-66572915	MS.gene20705:CDS
GTGCCAGCAGTGTTCATTGG+TGG	0.525941	1.2:-66572871	MS.gene20705:CDS
TCAAGTAATGGATAGAGTGA+AGG	0.542952	1.2:-66573070	None:intergenic
CTCACTCAAACAAATGCTCA+TGG	0.566311	1.2:-66572800	MS.gene20705:CDS
TCATTGGTGGAAAATTTGTA+GGG	0.566956	1.2:-66572858	MS.gene20705:CDS
TAGTTATTACTAGAACCAAA+TGG	0.577198	1.2:+66572768	None:intergenic
ACACAATTGTTCTATGAGCT+AGG	0.582609	1.2:-66572982	MS.gene20705:CDS
AAAATTTGTAGGGTCATCAA+AGG	0.583330	1.2:-66572848	MS.gene20705:CDS
TTGACAATGAACCCTATGGT+AGG	0.584348	1.2:-66572936	MS.gene20705:CDS
GAGCTTGACAATGAACCCTA+TGG	0.586058	1.2:-66572940	MS.gene20705:CDS
TGACAATGAACCCTATGGTA+GGG	0.596777	1.2:-66572935	MS.gene20705:CDS
TGAACACTGCTGGCACTGAA+GGG	0.607813	1.2:+66572878	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAATTTGTAGGGTCATCAA+AGG	-	chr1.2:66572992-66573011	MS.gene20705:CDS	30.0%
	TCATTGGTGGAAAATTTGTA+GGG	-	chr1.2:66572982-66573001	MS.gene20705:CDS	30.0%
	TTCATTGGTGGAAAATTTGT+AGG	-	chr1.2:66572981-66573000	MS.gene20705:CDS	30.0%
	ACACAATTGTTCTATGAGCT+AGG	-	chr1.2:66572858-66572877	MS.gene20705:CDS	35.0%
	GTGATATCTCTTCATGTTGA+TGG	-	chr1.2:66573017-66573036	MS.gene20705:CDS	35.0%
	CTCACTCAAACAAATGCTCA+TGG	-	chr1.2:66573040-66573059	MS.gene20705:CDS	40.0%
	CTTTCCATTTCCCTACCATA+GGG	+	chr1.2:66572918-66572937	None:intergenic	40.0%
	TCTTTCCATTTCCCTACCAT+AGG	+	chr1.2:66572919-66572938	None:intergenic	40.0%
	TGACAATGAACCCTATGGTA+GGG	-	chr1.2:66572905-66572924	MS.gene20705:CDS	40.0%
	TTGACAATGAACCCTATGGT+AGG	-	chr1.2:66572904-66572923	MS.gene20705:CDS	40.0%
	AACAAATGCTCATGGCTGCT+AGG	-	chr1.2:66573048-66573067	MS.gene20705:CDS	45.0%
	ACAAATGCTCATGGCTGCTA+GGG	-	chr1.2:66573049-66573068	MS.gene20705:CDS	45.0%
	GAGCTTGACAATGAACCCTA+TGG	-	chr1.2:66572900-66572919	MS.gene20705:CDS	45.0%
	GGGAAATGGAAAGAGCTTTG+AGG	-	chr1.2:66572925-66572944	MS.gene20705:CDS	45.0%
	TGAACCCTATGGTAGGGAAA+TGG	-	chr1.2:66572911-66572930	MS.gene20705:CDS	45.0%
	TTTCCACCAATGAACACTGC+TGG	+	chr1.2:66572975-66572994	None:intergenic	45.0%
!!	GAAAGAGCTTTGAGGAGTCT+TGG	-	chr1.2:66572933-66572952	MS.gene20705:CDS	45.0%
	TCAGTGCCAGCAGTGTTCAT+TGG	-	chr1.2:66572966-66572985	MS.gene20705:CDS	50.0%
	TTGTCAAGCTCATGCACAGC+AGG	+	chr1.2:66572891-66572910	None:intergenic	50.0%
!	ATGAACACTGCTGGCACTGA+AGG	+	chr1.2:66572966-66572985	None:intergenic	50.0%
!	TGAACACTGCTGGCACTGAA+GGG	+	chr1.2:66572965-66572984	None:intergenic	50.0%
	GTGCCAGCAGTGTTCATTGG+TGG	-	chr1.2:66572969-66572988	MS.gene20705:CDS	55.0%
	TCATGGCTGCTAGGGCCATT+TGG	-	chr1.2:66573057-66573076	MS.gene20705:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	66572777	66573085	66572777	ID=MS.gene20705
chr1.2	mRNA	66572777	66573085	66572777	ID=MS.gene20705.t1;Parent=MS.gene20705
chr1.2	exon	66572777	66573085	66572777	ID=MS.gene20705.t1.exon1;Parent=MS.gene20705.t1
chr1.2	CDS	66572777	66573085	66572777	ID=cds.MS.gene20705.t1;Parent=MS.gene20705.t1

Gene Sequence	>MS.gene20705 ATGGATAGAGTGAAGGATTTGGCATCAAAGAAAGCTGCAGTTATATTCACAAAGAGTTCATGCTACATGTGTCACAGCATAACACAATTGTTCTATGAGCTAGGAGCAAGTCCTGCTGTGCATGAGCTTGACAATGAACCCTATGGTAGGGAAATGGAAAGAGCTTTGAGGAGTCTTGGATGTAACCCTTCAGTGCCAGCAGTGTTCATTGGTGGAAAATTTGTAGGGTCATCAAAGGATGTGATATCTCTTCATGTTGATGGCTCACTCAAACAAATGCTCATGGCTGCTAGGGCCATTTGGTTCTAG
Protein sequence	>MS.gene20705.t1 MDRVKDLASKKAAVIFTKSSCYMCHSITQLFYELGASPAVHELDNEPYGREMERALRSLGCNPSVPAVFIGGKFVGSSKDVISLHVDGSLKQMLMAARAIWF