AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene007459

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007459.t1 MTR_7g100760 99.400 500 3 0 1 500 1 500 0.0 1023
MS.gene007459.t1 MTR_4g083340 93.763 497 31 0 4 500 1 497 0.0 969
MS.gene007459.t1 MTR_4g083330 88.400 500 31 2 1 500 1 473 0.0 879
MS.gene007459.t1 MTR_6g004020 77.800 491 109 0 10 500 20 510 0.0 811
MS.gene007459.t1 MTR_7g103420 42.685 499 274 7 12 500 30 526 1.11e-128 384
MS.gene007459.t1 MTR_1g091040 41.553 515 283 9 1 500 14 525 4.44e-127 380
MS.gene007459.t1 MTR_7g103420 41.829 514 272 9 12 500 30 541 3.71e-126 379
MS.gene007459.t1 MTR_1g061630 42.659 504 269 10 12 500 30 528 4.27e-124 373
MS.gene007459.t1 MTR_1g061630 42.063 504 256 10 12 500 30 512 4.94e-122 367
MS.gene007459.t1 MTR_7g103420 40.319 439 250 7 72 500 1 437 3.37e-100 308
MS.gene007459.t1 MTR_1g091040 43.768 345 188 5 157 497 47 389 2.53e-92 286
MS.gene007459.t1 MTR_5g014140 35.759 481 274 11 6 482 73 522 3.29e-88 281
MS.gene007459.t1 MTR_6g034195 36.082 485 261 12 9 482 104 550 2.49e-84 271
MS.gene007459.t1 MTR_1g099360 35.052 485 266 12 9 482 102 548 6.09e-82 265
MS.gene007459.t1 MTR_5g014140 41.317 334 182 7 6 333 73 398 7.55e-78 250
MS.gene007459.t1 MTR_1g076540 32.136 501 281 12 11 485 90 557 1.04e-72 241
MS.gene007459.t1 MTR_1g105965 32.383 491 294 11 11 488 91 556 6.47e-71 236
MS.gene007459.t1 MTR_1g105965 35.577 312 187 7 11 313 91 397 7.40e-53 184
MS.gene007459.t1 MTR_1g105965 31.481 216 135 6 11 218 91 301 2.84e-23 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007459.t1 AT5G56350 90.081 494 49 0 7 500 5 498 0.0 919
MS.gene007459.t1 AT4G26390 84.306 497 78 0 4 500 1 497 0.0 877
MS.gene007459.t1 AT5G08570 78.208 491 107 0 10 500 20 510 0.0 814
MS.gene007459.t1 AT5G08570 78.208 491 107 0 10 500 20 510 0.0 814
MS.gene007459.t1 AT5G08570 78.208 491 107 0 10 500 20 510 0.0 814
MS.gene007459.t1 AT5G63680 78.093 493 108 0 8 500 18 510 0.0 813
MS.gene007459.t1 AT5G63680 78.093 493 108 0 8 500 18 510 0.0 813
MS.gene007459.t1 AT5G63680 78.093 493 108 0 8 500 18 510 0.0 813
MS.gene007459.t1 AT3G04050 74.597 496 122 3 9 500 15 510 0.0 762
MS.gene007459.t1 AT3G55650 72.177 496 134 3 9 500 15 510 0.0 733
MS.gene007459.t1 AT3G25960 72.874 494 121 4 9 500 15 497 0.0 732
MS.gene007459.t1 AT3G55810 68.952 496 132 4 9 500 15 492 0.0 682
MS.gene007459.t1 AT3G52990 43.912 501 265 9 12 500 30 526 3.23e-130 389
MS.gene007459.t1 AT2G36580 43.086 499 272 7 12 500 30 526 6.19e-129 385
MS.gene007459.t1 AT3G52990 43.187 477 255 9 36 500 1 473 4.36e-121 363
MS.gene007459.t1 AT5G52920 36.066 488 253 14 11 482 111 555 6.90e-83 268
MS.gene007459.t1 AT1G32440 39.563 412 228 10 7 412 96 492 2.90e-80 261
MS.gene007459.t1 AT3G22960 31.919 495 288 12 11 485 118 583 9.11e-72 239
MS.gene007459.t1 AT3G49160 31.855 248 148 7 91 322 349 591 1.27e-21 99.0
MS.gene007459.t1 AT3G49160 31.855 248 148 7 91 322 437 679 1.51e-21 99.0

Find 128 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAATTCAGATGCATTTA+TGG 0.114929 7.3:+12172902 MS.gene007459:CDS
AAATACATCTCCATAGTTAA+TGG 0.247321 7.3:-12173213 None:intergenic
CAAAGTTTGCAACCCCTTCT+TGG 0.251729 7.3:-12172869 None:intergenic
CAGGGAAGATATTATGGTTT+GGG 0.252468 7.3:+12172291 MS.gene007459:CDS
ACTATGGAGATGTATTTAAA+AGG 0.271975 7.3:+12173219 MS.gene007459:CDS
AGCTTCAGCTCTGGTTGGCC+TGG 0.294659 7.3:-12173063 None:intergenic
AGCTTCTTCTGCAGTTAAAA+TGG 0.294876 7.3:+12173286 MS.gene007459:CDS
GAAAGACAGGGAAGATATTA+TGG 0.300369 7.3:+12172285 MS.gene007459:CDS
AGCTAAAGCAGCACTTATAT+TGG 0.310879 7.3:+12173316 MS.gene007459:CDS
TGTGAGAATTCTGCTGTTCT+AGG 0.317802 7.3:+12172205 MS.gene007459:CDS
TGATGTCGCCAATGCAGTTC+TGG 0.323821 7.3:+12173088 MS.gene007459:CDS
CGATAAAACTGTAAATGATA+TGG 0.332818 7.3:-12172149 None:intergenic
CAAAATTAGTGGCTAAATAC+AGG 0.342121 7.3:+12173363 MS.gene007459:CDS
TGATCATTGACTCCAACATC+TGG 0.357864 7.3:-12173034 None:intergenic
TTTATCGTGTGACAAAGAAT+TGG 0.366431 7.3:+12172165 MS.gene007459:CDS
ATTGCAGAGACCCTTTGTTT+TGG 0.368851 7.3:-12173579 None:intergenic
AGAACTTGCTGTTCGAACTA+TGG 0.369701 7.3:+12173163 MS.gene007459:CDS
CAAAGGGTCTCTGCAATAAC+GGG 0.379781 7.3:+12173585 MS.gene007459:CDS
CGCCGTCATGCTCGATACTA+AGG 0.388196 7.3:+12171320 MS.gene007459:CDS
TCGTATTCTCATCACCCTTT+AGG 0.399274 7.3:-12172057 None:intergenic
ACAGGGAAGATATTATGGTT+TGG 0.412591 7.3:+12172290 MS.gene007459:CDS
GTTCTGATCTGGTGCAAGTT+CGG 0.424518 7.3:+12172362 MS.gene007459:CDS
GGAACGACAACAGAAAGAAT+TGG 0.428468 7.3:-12173395 None:intergenic
AGATGTATTTAAAAGGATAA+TGG 0.428807 7.3:+12173226 MS.gene007459:CDS
TTAAGACTGACACCTTTGAT+TGG 0.431136 7.3:+12173423 MS.gene007459:CDS
GCTTCAGCTCTGGTTGGCCT+GGG 0.431308 7.3:-12173062 None:intergenic
GCTGAGAGTACCATTAACTA+TGG 0.439742 7.3:+12173203 MS.gene007459:CDS
CAGTAACAACAGGCTTTCCT+TGG 0.442626 7.3:-12173007 None:intergenic
TGTTCGAAAAGGTTCTGATC+TGG 0.444704 7.3:+12172351 MS.gene007459:CDS
TTATCGTGTGACAAAGAATT+GGG 0.446712 7.3:+12172166 MS.gene007459:CDS
TTTCATTACCTTGTTTCAGT+TGG 0.451778 7.3:-12172012 None:intergenic
TTACCTTGTTTCAGTTGGAT+AGG 0.452533 7.3:-12172007 None:intergenic
TAGACCTAGATGCGGGTCCC+AGG 0.456358 7.3:-12171164 None:intergenic
TCCAATGGGCTCATGGGTAC+TGG 0.457116 7.3:-12173257 None:intergenic
GGCAGGGGCCTCATCACTGC+AGG 0.458802 7.3:-12173447 None:intergenic
ATCTGGGTTGCAGTAACAAC+AGG 0.461731 7.3:-12173017 None:intergenic
ACCATGAGAGAAGTTGAATC+TGG 0.466351 7.3:-12171229 None:intergenic
GCAGAACCTGCGCTCAGCAC+TGG 0.467151 7.3:-12173500 None:intergenic
ATAGTTCGAACAGCAAGTTC+TGG 0.469788 7.3:-12173161 None:intergenic
GCACCCTGGGACCCGCATCT+AGG 0.471777 7.3:+12171160 MS.gene007459:CDS
TTGGCTGAGGATGTGAAGCC+CGG 0.472020 7.3:+12172100 MS.gene007459:CDS
ACTAAGATCGTTTGCACCCT+GGG 0.474644 7.3:+12171147 MS.gene007459:CDS
GCCAGATTCAACTTCTCTCA+TGG 0.474681 7.3:+12171228 MS.gene007459:CDS
CCACTTTGACTGAGAAAGAC+AGG 0.477943 7.3:+12172272 MS.gene007459:CDS
TAGACTCTCCAATGGGCTCA+TGG 0.478114 7.3:-12173264 None:intergenic
CAATTGGAATTTCCATTCCA+AGG 0.480988 7.3:-12172938 None:intergenic
AGAGCTGCCATGGAGAATAC+TGG 0.485585 7.3:+12171288 MS.gene007459:CDS
GTGTGACAAAGAATTGGGTT+TGG 0.488491 7.3:+12172171 MS.gene007459:CDS
TCGGAATATCAAGCTATGTC+TGG 0.489128 7.3:-12173468 None:intergenic
AGGGAGTACTGCAAAATTAG+TGG 0.490170 7.3:+12173352 MS.gene007459:CDS
AGAAAGAATGTTAATCTGCC+TGG 0.492316 7.3:+12172232 MS.gene007459:CDS
AGAAATCCAGTACGAATCTC+AGG 0.495410 7.3:-12171971 None:intergenic
ACAAAGGGTCTCTGCAATAA+CGG 0.495790 7.3:+12173584 MS.gene007459:CDS
TCAACCGACTATAGCCTAAA+GGG 0.495846 7.3:+12172043 MS.gene007459:CDS
AATATCAAGCTATGTCTGGC+AGG 0.498082 7.3:-12173464 None:intergenic
AGTGTCATACTTTGCGCAGA+TGG 0.504536 7.3:+12172124 MS.gene007459:CDS
TTCAACCGACTATAGCCTAA+AGG 0.505055 7.3:+12172042 MS.gene007459:CDS
CAGGGGCCTGAGATTCGTAC+TGG 0.508790 7.3:+12171965 MS.gene007459:intron
TGTGGTGGCTCTGCATCGCG+TGG 0.510579 7.3:+12173613 MS.gene007459:CDS
TGGTTCTCATGAGTATCATC+AGG 0.514740 7.3:+12171248 MS.gene007459:CDS
TGCGCAAAGTATGACACTCC+CGG 0.522253 7.3:-12172119 None:intergenic
CGTACTGGATTTCTCAAGGA+TGG 0.522596 7.3:+12171980 MS.gene007459:CDS
CGTAAAAGCTTCTCGACCAT+GGG 0.524474 7.3:-12171189 None:intergenic
TTGATTCCAGTGCTGAGCGC+AGG 0.525636 7.3:+12173494 MS.gene007459:CDS
AAGGTGTCAGTCTTAATCTC+AGG 0.528436 7.3:-12173416 None:intergenic
AAGTGCTGCTTTAGCTGAGT+TGG 0.530010 7.3:-12173309 None:intergenic
CGCATCTAGGTCTATTCCCA+TGG 0.531643 7.3:+12171173 MS.gene007459:CDS
TGATAATCTTAGAGCTGCCA+TGG 0.531806 7.3:+12171278 MS.gene007459:CDS
GACAGGTTGAAAACCAAGAA+GGG 0.533301 7.3:+12172856 MS.gene007459:intron
TCAGTAGCTTCAGCTCTGGT+TGG 0.537028 7.3:-12173068 None:intergenic
CTTAGCGGTGAAACTGCTGC+TGG 0.542033 7.3:+12173131 MS.gene007459:CDS
AACTAAGATCGTTTGCACCC+TGG 0.542271 7.3:+12171146 MS.gene007459:CDS
ATTCAGTATGCCAAAACAAA+GGG 0.544551 7.3:+12173569 MS.gene007459:CDS
GATCATTGACTCCAACATCT+GGG 0.548144 7.3:-12173033 None:intergenic
GACATCAGTAGCTTCAGCTC+TGG 0.552264 7.3:-12173072 None:intergenic
GGAAGATCCACTACAACTCC+AGG 0.552416 7.3:-12172250 None:intergenic
TCGTAAAAGCTTCTCGACCA+TGG 0.552997 7.3:-12171190 None:intergenic
GAACATTCTTCTCATGTCAA+AGG 0.556045 7.3:+12172408 MS.gene007459:CDS
AGACTCTCCAATGGGCTCAT+GGG 0.558290 7.3:-12173263 None:intergenic
CTGTCTTTCTCAGTCAAAGT+GGG 0.559857 7.3:-12172271 None:intergenic
GTGCAAGTTCGGAAACTGCT+AGG 0.563474 7.3:+12172373 MS.gene007459:CDS
ATCACCCTTTAGGCTATAGT+CGG 0.565528 7.3:-12172047 None:intergenic
TGTGCCATCCAGAACTGCAT+TGG 0.567212 7.3:-12173096 None:intergenic
AGACCTAGATGCGGGTCCCA+GGG 0.567340 7.3:-12171163 None:intergenic
TGACAGGTTGAAAACCAAGA+AGG 0.568293 7.3:+12172855 MS.gene007459:intron
ACCAGTACCCATGAGCCCAT+TGG 0.569139 7.3:+12173256 MS.gene007459:CDS
ATATCAAGCTATGTCTGGCA+GGG 0.569800 7.3:-12173463 None:intergenic
CCTGTCTTTCTCAGTCAAAG+TGG 0.571808 7.3:-12172272 None:intergenic
CTGCAATAACGGGGATTCTG+TGG 0.578914 7.3:+12173595 MS.gene007459:CDS
GGCACGTGGTGACCTTGGAA+TGG 0.579482 7.3:+12172926 MS.gene007459:CDS
AAGAAGCTAGACTCTCCAAT+GGG 0.579536 7.3:-12173271 None:intergenic
GATTCGTACTGGATTTCTCA+AGG 0.582404 7.3:+12171976 MS.gene007459:CDS
ACAGACTGTGTCATGCTTAG+CGG 0.586904 7.3:+12173116 MS.gene007459:CDS
ATCACTGCAGGACCAATCAA+AGG 0.592052 7.3:-12173435 None:intergenic
CAATAACGGGGATTCTGTGG+TGG 0.592591 7.3:+12173598 MS.gene007459:CDS
TCAGCACTGGAATCAATCCT+CGG 0.594323 7.3:-12173487 None:intergenic
TAAATGCATCTGAATTTGCA+AGG 0.595113 7.3:-12172899 None:intergenic
GCGCAAAGTATGACACTCCC+GGG 0.596294 7.3:-12172118 None:intergenic
ATGGTGGCACGTGGTGACCT+TGG 0.597261 7.3:+12172921 MS.gene007459:CDS
ATTTATAAGTGCAATATCCA+AGG 0.598187 7.3:+12172990 MS.gene007459:CDS
AAATTCAGATGCATTTATGG+TGG 0.599650 7.3:+12172905 MS.gene007459:CDS
TGGCTGAGGATGTGAAGCCC+GGG 0.599847 7.3:+12172101 MS.gene007459:CDS
TCACACAGAAACAACAGAAG+AGG 0.601211 7.3:+12173532 MS.gene007459:CDS
TTAGTGGCTAAATACAGGGC+AGG 0.603386 7.3:+12173368 MS.gene007459:CDS
TGATTGGTCCTGCAGTGATG+AGG 0.607592 7.3:+12173439 MS.gene007459:CDS
TGTTACTGCAACCCAGATGT+TGG 0.608418 7.3:+12173022 MS.gene007459:CDS
CATTCAGTATGCCAAAACAA+AGG 0.608524 7.3:+12173568 MS.gene007459:CDS
TGAGAAGAATGTTCTTAGCA+TGG 0.609210 7.3:-12172399 None:intergenic
CACTTTGACTGAGAAAGACA+GGG 0.613117 7.3:+12172273 MS.gene007459:CDS
AGACATAGCTTGATATTCCG+AGG 0.613725 7.3:+12173470 MS.gene007459:CDS
TAATCTGCCTGGAGTTGTAG+TGG 0.614488 7.3:+12172243 MS.gene007459:CDS
GAAGAAGCTAGACTCTCCAA+TGG 0.622268 7.3:-12173272 None:intergenic
GTGGTGGCTCTGCATCGCGT+GGG 0.624225 7.3:+12173614 MS.gene007459:CDS
AGGGAAGATATTATGGTTTG+GGG 0.626962 7.3:+12172292 MS.gene007459:CDS
ATGGGAATAGACCTAGATGC+GGG 0.628393 7.3:-12171171 None:intergenic
AAGAATACCAGTATTCTCCA+TGG 0.629052 7.3:-12171295 None:intergenic
GTCGCCAATGCAGTTCTGGA+TGG 0.630881 7.3:+12173092 MS.gene007459:CDS
AAACCTATCCAACTGAAACA+AGG 0.639584 7.3:+12172004 MS.gene007459:CDS
TATCAAGCTATGTCTGGCAG+GGG 0.639890 7.3:-12173462 None:intergenic
AGTCAATGATCAAATCTCCC+AGG 0.644547 7.3:+12173045 MS.gene007459:CDS
AAAATTAGTGGCTAAATACA+GGG 0.653786 7.3:+12173364 MS.gene007459:CDS
ACAGGTTGAAAACCAAGAAG+GGG 0.682754 7.3:+12172857 MS.gene007459:intron
TAATCTAAAGATGTCGACGA+TGG 0.682849 7.3:+12171104 None:intergenic
TACCTTAGTATCGAGCATGA+CGG 0.683613 7.3:-12171322 None:intergenic
CATGGGAATAGACCTAGATG+CGG 0.689751 7.3:-12171172 None:intergenic
AAAGGGTCTCTGCAATAACG+GGG 0.712446 7.3:+12173586 MS.gene007459:CDS
GATGCATTTATGGTGGCACG+TGG 0.737087 7.3:+12172912 MS.gene007459:CDS
GAACAGCAGAATTCTCACAG+CGG 0.826305 7.3:-12172201 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAATAAATAAAAAAA+GGG - chr7.3:12171353-12171372 None:intergenic 0.0%
!! TAAATAAAATAAATAAAAAA+AGG - chr7.3:12171354-12171373 None:intergenic 0.0%
!! AAATGAAAGATATGAAATGA+AGG - chr7.3:12171916-12171935 None:intergenic 20.0%
!! AGATGTATTTAAAAGGATAA+TGG + chr7.3:12173226-12173245 MS.gene007459:CDS 20.0%
!! AGTAGACATAAGATTATATA+AGG - chr7.3:12171485-12171504 None:intergenic 20.0%
!! ATATAATCTTATGTCTACTA+TGG + chr7.3:12171486-12171505 MS.gene007459:intron 20.0%
!! TTTGAAAAATTATGGATTTG+AGG + chr7.3:12172688-12172707 MS.gene007459:intron 20.0%
!!! TTTCTGATTTTTTTTTTCCA+TGG + chr7.3:12172447-12172466 MS.gene007459:intron 20.0%
!!! TTTTTTCTAGGACAAAATTT+AGG + chr7.3:12172633-12172652 MS.gene007459:intron 20.0%
!!! TTTTTTTTTCCATGGAAAAT+AGG + chr7.3:12172455-12172474 MS.gene007459:intron 20.0%
! AAAATTAGTGGCTAAATACA+GGG + chr7.3:12173364-12173383 MS.gene007459:CDS 25.0%
! AAATACATCTCCATAGTTAA+TGG - chr7.3:12173216-12173235 None:intergenic 25.0%
! AAATTTAGGCACATTTCTTT+AGG + chr7.3:12172647-12172666 MS.gene007459:intron 25.0%
! AACTATACAAAAAACACCTA+AGG + chr7.3:12172734-12172753 MS.gene007459:intron 25.0%
! ACTATGGAGATGTATTTAAA+AGG + chr7.3:12173219-12173238 MS.gene007459:CDS 25.0%
! ATTTATAAGTGCAATATCCA+AGG + chr7.3:12172990-12173009 MS.gene007459:CDS 25.0%
! CCAATATAACACACATTAAT+AGG - chr7.3:12172555-12172574 None:intergenic 25.0%
! CCTATTAATGTGTGTTATAT+TGG + chr7.3:12172552-12172571 MS.gene007459:intron 25.0%
! CGATAAAACTGTAAATGATA+TGG - chr7.3:12172152-12172171 None:intergenic 25.0%
!! AAAAAATCACCTATTTTCCA+TGG - chr7.3:12172467-12172486 None:intergenic 25.0%
!! AGGCAAACTTTGAAAAATTA+TGG + chr7.3:12172680-12172699 MS.gene007459:intron 25.0%
!! GCAATCATGTCAATTTTATT+GGG - chr7.3:12172322-12172341 None:intergenic 25.0%
!! GCTAGAAATATTTTCTCAAT+TGG - chr7.3:12172957-12172976 None:intergenic 25.0%
!!! CTTATATTGGTTTTAACTAG+AGG + chr7.3:12173329-12173348 MS.gene007459:CDS 25.0%
!!! TTTCTTTAGGTGTTTTTTGT+AGG + chr7.3:12172660-12172679 MS.gene007459:intron 25.0%
AAAACTTAGTGCATTTCCTT+AGG - chr7.3:12172753-12172772 None:intergenic 30.0%
AAATTCAGATGCATTTATGG+TGG + chr7.3:12172905-12172924 MS.gene007459:CDS 30.0%
AGAACTAAAGAAAGTCTGTT+TGG - chr7.3:12171740-12171759 None:intergenic 30.0%
ATTCAGTATGCCAAAACAAA+GGG + chr7.3:12173569-12173588 MS.gene007459:CDS 30.0%
CAAAATTAGTGGCTAAATAC+AGG + chr7.3:12173363-12173382 MS.gene007459:CDS 30.0%
CATCAATTTGCAATGTAAGT+AGG - chr7.3:12171945-12171964 None:intergenic 30.0%
CATGAATGGCTTAATCATTT+AGG + chr7.3:12171784-12171803 MS.gene007459:intron 30.0%
TAAATGCATCTGAATTTGCA+AGG - chr7.3:12172902-12172921 None:intergenic 30.0%
TCATAAGTCAAGAACATGAA+TGG + chr7.3:12171770-12171789 MS.gene007459:intron 30.0%
TGCAAATTCAGATGCATTTA+TGG + chr7.3:12172902-12172921 MS.gene007459:CDS 30.0%
TTACATTGCAAATTGATGCA+GGG + chr7.3:12171947-12171966 MS.gene007459:intron 30.0%
TTATCGTGTGACAAAGAATT+GGG + chr7.3:12172166-12172185 MS.gene007459:CDS 30.0%
TTTATCGTGTGACAAAGAAT+TGG + chr7.3:12172165-12172184 MS.gene007459:CDS 30.0%
! AGTGTTTATGGTAGTGAATA+AGG - chr7.3:12171564-12171583 None:intergenic 30.0%
! CGCAATCATGTCAATTTTAT+TGG - chr7.3:12172323-12172342 None:intergenic 30.0%
! GAATCTGTTATTTGTCGTAA+AGG + chr7.3:12171516-12171535 MS.gene007459:intron 30.0%
! TTTCATTACCTTGTTTCAGT+TGG - chr7.3:12172015-12172034 None:intergenic 30.0%
!!! AGTTGCTACTGATTTTTTCT+AGG + chr7.3:12172621-12172640 MS.gene007459:intron 30.0%
!!! ATATTGGTTTTAACTAGAGG+AGG + chr7.3:12173332-12173351 MS.gene007459:CDS 30.0%
!!! TATTGGTTTTAACTAGAGGA+GGG + chr7.3:12173333-12173352 MS.gene007459:CDS 30.0%
AAACCTATCCAACTGAAACA+AGG + chr7.3:12172004-12172023 MS.gene007459:CDS 35.0%
AACTTGCAAATGTATGTGAC+AGG + chr7.3:12172839-12172858 MS.gene007459:intron 35.0%
AAGAATACCAGTATTCTCCA+TGG - chr7.3:12171298-12171317 None:intergenic 35.0%
ACAGGGAAGATATTATGGTT+TGG + chr7.3:12172290-12172309 MS.gene007459:CDS 35.0%
AGAAAGAATGTTAATCTGCC+TGG + chr7.3:12172232-12172251 MS.gene007459:CDS 35.0%
AGCTAAAGCAGCACTTATAT+TGG + chr7.3:12173316-12173335 MS.gene007459:CDS 35.0%
AGCTTCTTCTGCAGTTAAAA+TGG + chr7.3:12173286-12173305 MS.gene007459:CDS 35.0%
AGGGAAGATATTATGGTTTG+GGG + chr7.3:12172292-12172311 MS.gene007459:CDS 35.0%
ATGAAGGAACTTGAAAATGC+TGG - chr7.3:12171900-12171919 None:intergenic 35.0%
CAATTGGAATTTCCATTCCA+AGG - chr7.3:12172941-12172960 None:intergenic 35.0%
CAGGGAAGATATTATGGTTT+GGG + chr7.3:12172291-12172310 MS.gene007459:CDS 35.0%
CATGCAAGCGATAGAAAAAA+AGG - chr7.3:12172778-12172797 None:intergenic 35.0%
CATTCAGTATGCCAAAACAA+AGG + chr7.3:12173568-12173587 MS.gene007459:CDS 35.0%
CTTACATTGCAAATTGATGC+AGG + chr7.3:12171946-12171965 MS.gene007459:intron 35.0%
GAAAGACAGGGAAGATATTA+TGG + chr7.3:12172285-12172304 MS.gene007459:CDS 35.0%
GAACATTCTTCTCATGTCAA+AGG + chr7.3:12172408-12172427 MS.gene007459:CDS 35.0%
TACATTGCAAATTGATGCAG+GGG + chr7.3:12171948-12171967 MS.gene007459:intron 35.0%
TATTTGTCGTAAAGGCTGTT+TGG + chr7.3:12171524-12171543 MS.gene007459:intron 35.0%
TGAGAAGAATGTTCTTAGCA+TGG - chr7.3:12172402-12172421 None:intergenic 35.0%
TTAAGACTGACACCTTTGAT+TGG + chr7.3:12173423-12173442 MS.gene007459:CDS 35.0%
! TTACCTTGTTTCAGTTGGAT+AGG - chr7.3:12172010-12172029 None:intergenic 35.0%
!! AACAGTCTCAGAAGTGTTTA+TGG - chr7.3:12171576-12171595 None:intergenic 35.0%
!! GTGCAAACGATCTTAGTTTT+CGG - chr7.3:12171144-12171163 None:intergenic 35.0%
!!! CTTTGTTTTGGCATACTGAA+TGG - chr7.3:12173570-12173589 None:intergenic 35.0%
AAATGAGCGCTTGTCATGAT+AGG + chr7.3:12171679-12171698 MS.gene007459:intron 40.0%
AAGAAGCTAGACTCTCCAAT+GGG - chr7.3:12173274-12173293 None:intergenic 40.0%
AATATCAAGCTATGTCTGGC+AGG - chr7.3:12173467-12173486 None:intergenic 40.0%
ACAAAGGGTCTCTGCAATAA+CGG + chr7.3:12173584-12173603 MS.gene007459:CDS 40.0%
ACCATGAGAGAAGTTGAATC+TGG - chr7.3:12171232-12171251 None:intergenic 40.0%
AGAAATCCAGTACGAATCTC+AGG - chr7.3:12171974-12171993 None:intergenic 40.0%
AGACATAGCTTGATATTCCG+AGG + chr7.3:12173470-12173489 MS.gene007459:CDS 40.0%
AGTCAATGATCAAATCTCCC+AGG + chr7.3:12173045-12173064 MS.gene007459:CDS 40.0%
ATAGTTCGAACAGCAAGTTC+TGG - chr7.3:12173164-12173183 None:intergenic 40.0%
ATATCAAGCTATGTCTGGCA+GGG - chr7.3:12173466-12173485 None:intergenic 40.0%
ATCACCCTTTAGGCTATAGT+CGG - chr7.3:12172050-12172069 None:intergenic 40.0%
CACTTTGACTGAGAAAGACA+GGG + chr7.3:12172273-12172292 MS.gene007459:CDS 40.0%
CATACATTTGCAAGTTGCAG+AGG - chr7.3:12172835-12172854 None:intergenic 40.0%
CTGCATGAGCTACAAAAAGT+TGG + chr7.3:12172081-12172100 MS.gene007459:CDS 40.0%
CTGTCTTTCTCAGTCAAAGT+GGG - chr7.3:12172274-12172293 None:intergenic 40.0%
GATTCGTACTGGATTTCTCA+AGG + chr7.3:12171976-12171995 MS.gene007459:CDS 40.0%
GGAACGACAACAGAAAGAAT+TGG - chr7.3:12173398-12173417 None:intergenic 40.0%
GTGTGACAAAGAATTGGGTT+TGG + chr7.3:12172171-12172190 MS.gene007459:CDS 40.0%
TCAACCGACTATAGCCTAAA+GGG + chr7.3:12172043-12172062 MS.gene007459:CDS 40.0%
TCACACAGAAACAACAGAAG+AGG + chr7.3:12173532-12173551 MS.gene007459:CDS 40.0%
TCGGAATATCAAGCTATGTC+TGG - chr7.3:12173471-12173490 None:intergenic 40.0%
TCGTATTCTCATCACCCTTT+AGG - chr7.3:12172060-12172079 None:intergenic 40.0%
TCTTTCACTGCATGCTGAAT+TGG + chr7.3:12171823-12171842 MS.gene007459:intron 40.0%
TGATAATCTTAGAGCTGCCA+TGG + chr7.3:12171278-12171297 MS.gene007459:CDS 40.0%
TGGTTCTCATGAGTATCATC+AGG + chr7.3:12171248-12171267 MS.gene007459:CDS 40.0%
TGTGAGAATTCTGCTGTTCT+AGG + chr7.3:12172205-12172224 MS.gene007459:CDS 40.0%
TTCAACCGACTATAGCCTAA+AGG + chr7.3:12172042-12172061 MS.gene007459:CDS 40.0%
TTCAGCATGCAGTGAAAGAT+AGG - chr7.3:12171822-12171841 None:intergenic 40.0%
! AAGGTGTCAGTCTTAATCTC+AGG - chr7.3:12173419-12173438 None:intergenic 40.0%
! ACAGGTTGAAAACCAAGAAG+GGG + chr7.3:12172857-12172876 MS.gene007459:intron 40.0%
! GACAGGTTGAAAACCAAGAA+GGG + chr7.3:12172856-12172875 MS.gene007459:intron 40.0%
! GCGCTATCTTTTGTTCGAAA+AGG + chr7.3:12172340-12172359 MS.gene007459:CDS 40.0%
! TACCTTAGTATCGAGCATGA+CGG - chr7.3:12171325-12171344 None:intergenic 40.0%
! TGACAGGTTGAAAACCAAGA+AGG + chr7.3:12172855-12172874 MS.gene007459:intron 40.0%
! TGTTCGAAAAGGTTCTGATC+TGG + chr7.3:12172351-12172370 MS.gene007459:CDS 40.0%
!! AGAACTTGCTGTTCGAACTA+TGG + chr7.3:12173163-12173182 MS.gene007459:CDS 40.0%
!! AGGGAGTACTGCAAAATTAG+TGG + chr7.3:12173352-12173371 MS.gene007459:CDS 40.0%
!! ATTGCAGAGACCCTTTGTTT+TGG - chr7.3:12173582-12173601 None:intergenic 40.0%
!! GATCATTGACTCCAACATCT+GGG - chr7.3:12173036-12173055 None:intergenic 40.0%
!! GCTGAGAGTACCATTAACTA+TGG + chr7.3:12173203-12173222 MS.gene007459:CDS 40.0%
!! TGATCATTGACTCCAACATC+TGG - chr7.3:12173037-12173056 None:intergenic 40.0%
AAAGGGTCTCTGCAATAACG+GGG + chr7.3:12173586-12173605 MS.gene007459:CDS 45.0%
AACTAAGATCGTTTGCACCC+TGG + chr7.3:12171146-12171165 MS.gene007459:CDS 45.0%
ACAGACTGTGTCATGCTTAG+CGG + chr7.3:12173116-12173135 MS.gene007459:CDS 45.0%
ACTAAGATCGTTTGCACCCT+GGG + chr7.3:12171147-12171166 MS.gene007459:CDS 45.0%
AGTGTCATACTTTGCGCAGA+TGG + chr7.3:12172124-12172143 MS.gene007459:CDS 45.0%
ATCACTGCAGGACCAATCAA+AGG - chr7.3:12173438-12173457 None:intergenic 45.0%
ATCTGGGTTGCAGTAACAAC+AGG - chr7.3:12173020-12173039 None:intergenic 45.0%
ATGGGAATAGACCTAGATGC+GGG - chr7.3:12171174-12171193 None:intergenic 45.0%
CAAAGGGTCTCTGCAATAAC+GGG + chr7.3:12173585-12173604 MS.gene007459:CDS 45.0%
CAAAGTTTGCAACCCCTTCT+TGG - chr7.3:12172872-12172891 None:intergenic 45.0%
CAGTAACAACAGGCTTTCCT+TGG - chr7.3:12173010-12173029 None:intergenic 45.0%
CATGGGAATAGACCTAGATG+CGG - chr7.3:12171175-12171194 None:intergenic 45.0%
CCACTTTGACTGAGAAAGAC+AGG + chr7.3:12172272-12172291 MS.gene007459:CDS 45.0%
CCTGTCTTTCTCAGTCAAAG+TGG - chr7.3:12172275-12172294 None:intergenic 45.0%
CGTAAAAGCTTCTCGACCAT+GGG - chr7.3:12171192-12171211 None:intergenic 45.0%
CGTACTGGATTTCTCAAGGA+TGG + chr7.3:12171980-12171999 MS.gene007459:CDS 45.0%
GAACAGCAGAATTCTCACAG+CGG - chr7.3:12172204-12172223 None:intergenic 45.0%
GAAGAAGCTAGACTCTCCAA+TGG - chr7.3:12173275-12173294 None:intergenic 45.0%
GAGCTACAAAAAGTTGGCTG+AGG + chr7.3:12172087-12172106 MS.gene007459:CDS 45.0%
GCCAGATTCAACTTCTCTCA+TGG + chr7.3:12171228-12171247 MS.gene007459:CDS 45.0%
GTTCTGATCTGGTGCAAGTT+CGG + chr7.3:12172362-12172381 MS.gene007459:CDS 45.0%
TAATCTGCCTGGAGTTGTAG+TGG + chr7.3:12172243-12172262 MS.gene007459:CDS 45.0%
TATCAAGCTATGTCTGGCAG+GGG - chr7.3:12173465-12173484 None:intergenic 45.0%
TCGTAAAAGCTTCTCGACCA+TGG - chr7.3:12171193-12171212 None:intergenic 45.0%
TGTTACTGCAACCCAGATGT+TGG + chr7.3:12173022-12173041 MS.gene007459:CDS 45.0%
TTAGTGGCTAAATACAGGGC+AGG + chr7.3:12173368-12173387 MS.gene007459:CDS 45.0%
! TCAGCACTGGAATCAATCCT+CGG - chr7.3:12173490-12173509 None:intergenic 45.0%
!! AAGTGCTGCTTTAGCTGAGT+TGG - chr7.3:12173312-12173331 None:intergenic 45.0%
AGAGCTGCCATGGAGAATAC+TGG + chr7.3:12171288-12171307 MS.gene007459:CDS 50.0%
CGCATCTAGGTCTATTCCCA+TGG + chr7.3:12171173-12171192 MS.gene007459:CDS 50.0%
GACATCAGTAGCTTCAGCTC+TGG - chr7.3:12173075-12173094 None:intergenic 50.0%
GATGCATTTATGGTGGCACG+TGG + chr7.3:12172912-12172931 MS.gene007459:CDS 50.0%
GGAAGATCCACTACAACTCC+AGG - chr7.3:12172253-12172272 None:intergenic 50.0%
GTGCAAGTTCGGAAACTGCT+AGG + chr7.3:12172373-12172392 MS.gene007459:CDS 50.0%
TGATGTCGCCAATGCAGTTC+TGG + chr7.3:12173088-12173107 MS.gene007459:CDS 50.0%
TGCGCAAAGTATGACACTCC+CGG - chr7.3:12172122-12172141 None:intergenic 50.0%
TGTGCCATCCAGAACTGCAT+TGG - chr7.3:12173099-12173118 None:intergenic 50.0%
! AGACTCTCCAATGGGCTCAT+GGG - chr7.3:12173266-12173285 None:intergenic 50.0%
! CAATAACGGGGATTCTGTGG+TGG + chr7.3:12173598-12173617 MS.gene007459:CDS 50.0%
! CTGCAATAACGGGGATTCTG+TGG + chr7.3:12173595-12173614 MS.gene007459:CDS 50.0%
! GTCGAGAAGCTTTTACGAGC+AGG + chr7.3:12171195-12171214 MS.gene007459:CDS 50.0%
! TAGACTCTCCAATGGGCTCA+TGG - chr7.3:12173267-12173286 None:intergenic 50.0%
! TCAGTAGCTTCAGCTCTGGT+TGG - chr7.3:12173071-12173090 None:intergenic 50.0%
! TGATTGGTCCTGCAGTGATG+AGG + chr7.3:12173439-12173458 MS.gene007459:CDS 50.0%
ACCAGTACCCATGAGCCCAT+TGG + chr7.3:12173256-12173275 MS.gene007459:CDS 55.0%
CGCCGTCATGCTCGATACTA+AGG + chr7.3:12171320-12171339 MS.gene007459:CDS 55.0%
CTTAGCGGTGAAACTGCTGC+TGG + chr7.3:12173131-12173150 MS.gene007459:CDS 55.0%
GCGCAAAGTATGACACTCCC+GGG - chr7.3:12172121-12172140 None:intergenic 55.0%
TGGTGCGAGATGCGAGTTCA+AGG + chr7.3:12171843-12171862 MS.gene007459:intron 55.0%
TTGGCTGAGGATGTGAAGCC+CGG + chr7.3:12172100-12172119 MS.gene007459:CDS 55.0%
! TCCAATGGGCTCATGGGTAC+TGG - chr7.3:12173260-12173279 None:intergenic 55.0%
! TTGATTCCAGTGCTGAGCGC+AGG + chr7.3:12173494-12173513 MS.gene007459:CDS 55.0%
!! GTCGCCAATGCAGTTCTGGA+TGG + chr7.3:12173092-12173111 MS.gene007459:CDS 55.0%
AGACCTAGATGCGGGTCCCA+GGG - chr7.3:12171166-12171185 None:intergenic 60.0%
ATGGTGGCACGTGGTGACCT+TGG + chr7.3:12172921-12172940 MS.gene007459:CDS 60.0%
CAGGGGCCTGAGATTCGTAC+TGG + chr7.3:12171965-12171984 MS.gene007459:intron 60.0%
GGCACGTGGTGACCTTGGAA+TGG + chr7.3:12172926-12172945 MS.gene007459:CDS 60.0%
TAGACCTAGATGCGGGTCCC+AGG - chr7.3:12171167-12171186 None:intergenic 60.0%
TGGCTGAGGATGTGAAGCCC+GGG + chr7.3:12172101-12172120 MS.gene007459:CDS 60.0%
! AGCTTCAGCTCTGGTTGGCC+TGG - chr7.3:12173066-12173085 None:intergenic 60.0%
!! GCTTCAGCTCTGGTTGGCCT+GGG - chr7.3:12173065-12173084 None:intergenic 60.0%
GCAGAACCTGCGCTCAGCAC+TGG - chr7.3:12173503-12173522 None:intergenic 65.0%
! GTGGTGGCTCTGCATCGCGT+GGG + chr7.3:12173614-12173633 MS.gene007459:CDS 65.0%
! TGTGGTGGCTCTGCATCGCG+TGG + chr7.3:12173613-12173632 MS.gene007459:CDS 65.0%
GCACCCTGGGACCCGCATCT+AGG + chr7.3:12171160-12171179 MS.gene007459:CDS 70.0%
GGCAGGGGCCTCATCACTGC+AGG - chr7.3:12173450-12173469 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 12171114 12173673 12171114 ID=MS.gene007459
chr7.3 mRNA 12171114 12173673 12171114 ID=MS.gene007459.t1;Parent=MS.gene007459
chr7.3 exon 12171114 12171341 12171114 ID=MS.gene007459.t1.exon1;Parent=MS.gene007459.t1
chr7.3 CDS 12171114 12171341 12171114 ID=cds.MS.gene007459.t1;Parent=MS.gene007459.t1
chr7.3 exon 12171968 12172429 12171968 ID=MS.gene007459.t1.exon2;Parent=MS.gene007459.t1
chr7.3 CDS 12171968 12172429 12171968 ID=cds.MS.gene007459.t1;Parent=MS.gene007459.t1
chr7.3 exon 12172861 12173673 12172861 ID=MS.gene007459.t1.exon3;Parent=MS.gene007459.t1
chr7.3 CDS 12172861 12173673 12172861 ID=cds.MS.gene007459.t1;Parent=MS.gene007459.t1
Gene Sequence

>MS.gene007459

ATGTCGACGATGGTAGCAGAGAAGAAACCGAAAACTAAGATCGTTTGCACCCTGGGACCCGCATCTAGGTCTATTCCCATGGTCGAGAAGCTTTTACGAGCAGGTATGAACGTTGCCAGATTCAACTTCTCTCATGGTTCTCATGAGTATCATCAGGAAACTCTTGATAATCTTAGAGCTGCCATGGAGAATACTGGTATTCTTTGCGCCGTCATGCTCGATACTAAGGTATAAAACCCTTTTTTTATTTATTTTATTTATTATGCATCTTTAAGTTTTCTTTGTTAATGCTTTTGATTTGAATTGATAGTGTATGTATGTAATTTGTGTTTGTTATTGATGTGTGAGATGTATCAAGTTGATTTTGACCTTATATAATCTTATGTCTACTATGGATCATAAGAATCTGTTATTTGTCGTAAAGGCTGTTTGGATTGACTTATTTCACCTTATTCACTACCATAAACACTTCTGAGACTGTTTGAGAGAAGTTGTGAAAATAATTTGTGATATGTTCATGAGTTAGTTAAACTTTATTTTATCTTTTGTTATAGAAATAACTTATAAATGAGCGCTTGTCATGATAGGTGATTATGCTATAAACTGCAAATTAAGTTGTTTATCCAAACAGACTTTCTTTAGTTCTTTTAATGTTTTCATAAGTCAAGAACATGAATGGCTTAATCATTTAGGCATATTACATTTCCTATCTTTCACTGCATGCTGAATTGGTGCGAGATGCGAGTTCAAGGCATTATTATTCATGAACTTAATAAAGTGAAACCAGCATTTTCAAGTTCCTTCATTTCATATCTTTCATTTTATGCACCTACTTACATTGCAAATTGATGCAGGGGCCTGAGATTCGTACTGGATTTCTCAAGGATGGCAAACCTATCCAACTGAAACAAGGTAATGAAATAACTATTTCAACCGACTATAGCCTAAAGGGTGATGAGAATACGATCTGCATGAGCTACAAAAAGTTGGCTGAGGATGTGAAGCCCGGGAGTGTCATACTTTGCGCAGATGGAACCATATCATTTACAGTTTTATCGTGTGACAAAGAATTGGGTTTGGTTCGAGTCCGCTGTGAGAATTCTGCTGTTCTAGGCGAGAGAAAGAATGTTAATCTGCCTGGAGTTGTAGTGGATCTTCCCACTTTGACTGAGAAAGACAGGGAAGATATTATGGTTTGGGGTGTTCCCAATAAAATTGACATGATTGCGCTATCTTTTGTTCGAAAAGGTTCTGATCTGGTGCAAGTTCGGAAACTGCTAGGACACCATGCTAAGAACATTCTTCTCATGTCAAAGGTATGCTATATTATGAATTTCTGATTTTTTTTTTCCATGGAAAATAGGTGATTTTTTAGCTTGTTTAACAATGTGTCAAGAGTTCTAATCAGACATGATATCATAGAGATGTTTGCTGCATTCCTATTAATGTGTGTTATATTGGACATCATCTAACAAAATTTGAGATGCTCTTCAAAGAAAATATGTTGAGTTGCTACTGATTTTTTCTAGGACAAAATTTAGGCACATTTCTTTAGGTGTTTTTTGTAGGCAAACTTTGAAAAATTATGGATTTGAGGAAATCATAGATAGTTTAGTTAAGAACTATACAAAAAACACCTAAGGAAATGCACTAAGTTTTTTCCTTTTTTTCTATCGCTTGCATGTGTTATGTTCAATTACTTATTTCTAGTTGCTTTTCCTCTGCAACTTGCAAATGTATGTGACAGGTTGAAAACCAAGAAGGGGTTGCAAACTTTGATGATATCCTTGCAAATTCAGATGCATTTATGGTGGCACGTGGTGACCTTGGAATGGAAATTCCAATTGAGAAAATATTTCTAGCACAGAAAGTGATGATTTATAAGTGCAATATCCAAGGAAAGCCTGTTGTTACTGCAACCCAGATGTTGGAGTCAATGATCAAATCTCCCAGGCCAACCAGAGCTGAAGCTACTGATGTCGCCAATGCAGTTCTGGATGGCACAGACTGTGTCATGCTTAGCGGTGAAACTGCTGCTGGAGCTTATCCAGAACTTGCTGTTCGAACTATGGCTAAAATTTGTGTTGAAGCTGAGAGTACCATTAACTATGGAGATGTATTTAAAAGGATAATGGAGCACTCACCAGTACCCATGAGCCCATTGGAGAGTCTAGCTTCTTCTGCAGTTAAAATGGCCAACTCAGCTAAAGCAGCACTTATATTGGTTTTAACTAGAGGAGGGAGTACTGCAAAATTAGTGGCTAAATACAGGGCAGGCATGCCAATTCTTTCTGTTGTCGTTCCTGAGATTAAGACTGACACCTTTGATTGGTCCTGCAGTGATGAGGCCCCTGCCAGACATAGCTTGATATTCCGAGGATTGATTCCAGTGCTGAGCGCAGGTTCTGCTAGAGCTTCTCACACAGAAACAACAGAAGAGGCACTAGACTTTGCCATTCAGTATGCCAAAACAAAGGGTCTCTGCAATAACGGGGATTCTGTGGTGGCTCTGCATCGCGTGGGTGTCGCATCAGTCATCAAAATCTTGACTGTGAAATGA
Protein sequence

>MS.gene007459.t1

MSTMVAEKKPKTKIVCTLGPASRSIPMVEKLLRAGMNVARFNFSHGSHEYHQETLDNLRAAMENTGILCAVMLDTKGPEIRTGFLKDGKPIQLKQGNEITISTDYSLKGDENTICMSYKKLAEDVKPGSVILCADGTISFTVLSCDKELGLVRVRCENSAVLGERKNVNLPGVVVDLPTLTEKDREDIMVWGVPNKIDMIALSFVRKGSDLVQVRKLLGHHAKNILLMSKVENQEGVANFDDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTINYGDVFKRIMEHSPVPMSPLESLASSAVKMANSAKAALILVLTRGGSTAKLVAKYRAGMPILSVVVPEIKTDTFDWSCSDEAPARHSLIFRGLIPVLSAGSARASHTETTEEALDFAIQYAKTKGLCNNGDSVVALHRVGVASVIKILTVK