AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82768

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82768.t1 MTR_6g004020 99.020 510 5 0 1 510 1 510 0.0 1041
MS.gene82768.t1 MTR_4g083340 78.004 491 108 0 20 510 7 497 0.0 815
MS.gene82768.t1 MTR_7g100760 77.597 491 110 0 20 510 10 500 0.0 806
MS.gene82768.t1 MTR_4g083330 74.949 491 96 2 20 510 10 473 0.0 741
MS.gene82768.t1 MTR_7g103420 44.153 496 263 9 22 506 30 522 1.00e-133 398
MS.gene82768.t1 MTR_1g091040 43.232 495 268 7 22 506 30 521 5.55e-133 396
MS.gene82768.t1 MTR_7g103420 43.249 511 261 11 22 506 30 537 4.79e-131 391
MS.gene82768.t1 MTR_1g061630 43.461 497 267 6 22 506 30 524 5.52e-129 386
MS.gene82768.t1 MTR_1g061630 43.260 497 252 7 22 506 30 508 8.72e-127 379
MS.gene82768.t1 MTR_7g103420 41.284 436 242 9 82 506 1 433 2.43e-104 319
MS.gene82768.t1 MTR_1g091040 43.526 363 199 5 148 506 28 388 5.79e-99 304
MS.gene82768.t1 MTR_5g014140 36.920 474 268 11 21 492 78 522 9.40e-85 272
MS.gene82768.t1 MTR_1g099360 36.875 480 260 12 21 492 104 548 1.13e-84 273
MS.gene82768.t1 MTR_6g034195 36.175 481 262 12 21 492 106 550 1.05e-83 270
MS.gene82768.t1 MTR_5g014140 41.590 327 181 6 21 343 78 398 1.72e-74 242
MS.gene82768.t1 MTR_1g076540 30.661 499 291 11 21 495 90 557 5.93e-73 242
MS.gene82768.t1 MTR_1g105965 32.172 488 293 11 21 495 91 553 7.14e-73 242
MS.gene82768.t1 MTR_1g105965 33.974 312 192 6 21 323 91 397 4.04e-54 187
MS.gene82768.t1 MTR_1g105965 29.630 216 139 5 21 228 91 301 6.01e-25 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82768.t1 AT5G08570 88.039 510 61 0 1 510 1 510 0.0 946
MS.gene82768.t1 AT5G08570 88.039 510 61 0 1 510 1 510 0.0 946
MS.gene82768.t1 AT5G08570 88.039 510 61 0 1 510 1 510 0.0 946
MS.gene82768.t1 AT5G63680 87.059 510 66 0 1 510 1 510 0.0 934
MS.gene82768.t1 AT5G63680 87.059 510 66 0 1 510 1 510 0.0 934
MS.gene82768.t1 AT5G63680 87.059 510 66 0 1 510 1 510 0.0 934
MS.gene82768.t1 AT5G56350 79.430 491 101 0 20 510 8 498 0.0 816
MS.gene82768.t1 AT4G26390 77.800 491 109 0 20 510 7 497 0.0 800
MS.gene82768.t1 AT3G04050 69.565 506 149 4 6 506 1 506 0.0 721
MS.gene82768.t1 AT3G55650 67.992 503 157 3 12 510 8 510 0.0 699
MS.gene82768.t1 AT3G25960 69.261 501 141 4 12 510 8 497 0.0 699
MS.gene82768.t1 AT3G55810 65.992 494 146 4 21 510 17 492 0.0 650
MS.gene82768.t1 AT2G36580 44.848 495 261 8 22 506 30 522 4.55e-135 401
MS.gene82768.t1 AT3G52990 44.646 495 262 8 22 506 30 522 1.09e-134 400
MS.gene82768.t1 AT3G52990 43.312 471 255 8 46 506 1 469 3.66e-124 372
MS.gene82768.t1 AT1G32440 38.958 480 260 13 15 492 95 543 3.57e-87 279
MS.gene82768.t1 AT5G52920 36.287 474 271 11 21 492 111 555 2.17e-83 270
MS.gene82768.t1 AT3G22960 30.972 494 294 11 21 495 118 583 3.64e-72 240
MS.gene82768.t1 AT3G49160 34.783 207 122 6 135 332 389 591 9.05e-21 96.3
MS.gene82768.t1 AT3G49160 34.783 207 122 6 135 332 477 679 1.01e-20 96.3

Find 129 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAAAATTGTTTGTACTTT+GGG 0.213355 6.1:+76362 MS.gene82768:CDS
GAGTTTCGCTCTGGCCTTAT+TGG 0.243879 6.1:-78475 None:intergenic
ACCCAAAGTACAAACAATTT+TGG 0.262629 6.1:-76363 None:intergenic
CAGAGGCTTGATTCCTATAT+TGG 0.264607 6.1:+78653 MS.gene82768:CDS
TGTCCTTCTTTGAGTTGAAT+AGG 0.285375 6.1:-77305 None:intergenic
TATATTAAGCTGTGCCTTGC+TGG 0.290018 6.1:-78630 None:intergenic
TCATTTCAAGGTTGAGAATC+AGG 0.297800 6.1:+78017 MS.gene82768:intron
GTTGTTGCTCTTCATCGTAT+TGG 0.325415 6.1:+78780 MS.gene82768:CDS
GACCAAAATTGTTTGTACTT+TGG 0.339741 6.1:+76361 MS.gene82768:CDS
GCAGAATCATCCCTTGATTA+TGG 0.346101 6.1:+78369 MS.gene82768:CDS
TGCTGTACGCACAGCCAATA+AGG 0.350511 6.1:+78461 MS.gene82768:CDS
GCCAGGAAAATCTTCTCAAC+TGG 0.350809 6.1:-78120 None:intergenic
GTTAATGTCCGCAAGGTTCT+CGG 0.353104 6.1:+77671 MS.gene82768:CDS
GCAATCATATCAATATTGTT+AGG 0.355092 6.1:-77617 None:intergenic
TGCTCTTTCATTTGTTCGTA+AGG 0.360649 6.1:+77637 MS.gene82768:CDS
ATTTATAAGTGTAATCTTGT+TGG 0.364853 6.1:+78156 MS.gene82768:CDS
TGACCACAGACTCATTTGAT+TGG 0.381256 6.1:+78589 MS.gene82768:CDS
GCAGATCCTTCTGCCAATAT+AGG 0.382735 6.1:-78666 None:intergenic
GAGCATAGAATGGTATTTCC+AGG 0.388661 6.1:-77416 None:intergenic
TGATCATGGATTCAAGCATC+TGG 0.395013 6.1:-78200 None:intergenic
CTACATCAGTTGCTTCGGCT+CGG 0.397785 6.1:-78239 None:intergenic
AGGCTTTCCAATGGACTCAT+AGG 0.405256 6.1:-78429 None:intergenic
TGCTGGTGCCTCATCACTGC+AGG 0.408792 6.1:-78613 None:intergenic
GCGTGAGACTGATGCGTTCA+TGG 0.409117 6.1:+78068 MS.gene82768:CDS
CGGACTGGCTTTCTGAAAGA+TGG 0.416803 6.1:+77278 MS.gene82768:CDS
ATGGTGGCCCGTGGTGATCT+TGG 0.421038 6.1:+78087 MS.gene82768:CDS
AGAATCTACAGATGCAATTC+TGG 0.426172 6.1:+78704 MS.gene82768:CDS
TAAGGAAGATATTCTTGAAT+GGG 0.428016 6.1:+77589 MS.gene82768:CDS
TACATCAGTTGCTTCGGCTC+GGG 0.429321 6.1:-78238 None:intergenic
GATTTCATAATCAGTAGTTA+TGG 0.432621 6.1:-77336 None:intergenic
CTTAAGTCAGCAACACAAAA+GGG 0.437722 6.1:+78735 MS.gene82768:CDS
AATCTTGTCTGTGGTAGTTC+CGG 0.438220 6.1:+78563 MS.gene82768:CDS
CTGCCTGTCCACTTGAAGCC+TGG 0.442454 6.1:+77398 MS.gene82768:CDS
CACGACCATCGTTTGGTAGT+TGG 0.447300 6.1:-76331 None:intergenic
AACTACTGATTATGAAATCA+AGG 0.451183 6.1:+77340 MS.gene82768:CDS
AGAAAGCCAGTCCGAATCTC+AGG 0.453908 6.1:-77269 None:intergenic
GTAGCCAATGCAGTTCTTGA+TGG 0.460232 6.1:+78258 MS.gene82768:CDS
TGAATATAGCACCATAATCA+AGG 0.462445 6.1:-78380 None:intergenic
AATGAGTCTGTGGTCAAAAC+CGG 0.463086 6.1:-78582 None:intergenic
CTGTCAAAACTCTGCAATGT+TGG 0.465660 6.1:+77502 MS.gene82768:CDS
TCTTAAGTCAGCAACACAAA+AGG 0.468356 6.1:+78734 MS.gene82768:CDS
ATTGGCTACATCAGTTGCTT+CGG 0.471027 6.1:-78244 None:intergenic
CCAAGTTAGTTGCTAAGTAT+AGG 0.476764 6.1:+78529 MS.gene82768:CDS
TTAGGAACACGACCATCGTT+TGG 0.481093 6.1:-76338 None:intergenic
CAAAAGGGGCTTTGTAAACC+TGG 0.486157 6.1:+78750 MS.gene82768:CDS
TCCATTGCCTATGAGTCCAT+TGG 0.486716 6.1:+78422 MS.gene82768:CDS
CAGGGGCCTGAGATTCGGAC+TGG 0.487007 6.1:+77263 MS.gene82768:intron
TTAATGTCCGCAAGGTTCTC+GGG 0.488664 6.1:+77672 MS.gene82768:CDS
GGAACTACCACAGACAAGAT+TGG 0.490094 6.1:-78561 None:intergenic
GCTTCGGCTCGGGTTGGCCG+AGG 0.492369 6.1:-78228 None:intergenic
TGTCAAAACTCTGCAATGTT+GGG 0.494616 6.1:+77503 MS.gene82768:CDS
ATAAGGAAGATATTCTTGAA+TGG 0.496374 6.1:+77588 MS.gene82768:CDS
CACTCTTACTGACAAGGATA+AGG 0.497352 6.1:+77571 MS.gene82768:CDS
CTTATCCTTGTCAGTAAGAG+TGG 0.498847 6.1:-77570 None:intergenic
ACCGACTGTGTCATGCTTAG+TGG 0.500132 6.1:+78282 MS.gene82768:CDS
CTATATTCAAAGAGATGATA+AGG 0.502593 6.1:+78394 MS.gene82768:CDS
GGAAGATCCACCACAACACC+AGG 0.502856 6.1:-77548 None:intergenic
TTTCCAGGCTTCAAGTGGAC+AGG 0.509567 6.1:-77401 None:intergenic
CTTAGTGGTGAAAGTGCTGC+TGG 0.513325 6.1:+78297 MS.gene82768:CDS
CAATCATATCAATATTGTTA+GGG 0.517496 6.1:-77616 None:intergenic
GCTCTTTCATTTGTTCGTAA+GGG 0.518972 6.1:+77638 MS.gene82768:CDS
TAACTCATTGATATCATCTC+CGG 0.519842 6.1:-77368 None:intergenic
TGGTACTCATGAATATCATC+AGG 0.520603 6.1:+76463 MS.gene82768:CDS
TTATGAAATCAAGGGAGATC+CGG 0.521337 6.1:+77349 MS.gene82768:CDS
TGAACTTGCTGTCAAAATCA+TGG 0.526619 6.1:+78329 MS.gene82768:CDS
CACGACAATGAGTTTCGCTC+TGG 0.526714 6.1:-78484 None:intergenic
AAGTCTGCTATGCATTCTAC+TGG 0.527158 6.1:+76503 MS.gene82768:CDS
AATACCATTCTATGCTCTGA+TGG 0.528435 6.1:+77422 MS.gene82768:CDS
TTCCATTCCAAGATCACCAC+GGG 0.528799 6.1:-78094 None:intergenic
ATGTCGAACATAGACATTGA+AGG 0.535384 6.1:+76299 MS.gene82768:CDS
ATCTCAACAGATCTCGAAGC+AGG 0.538705 6.1:-76386 None:intergenic
ACAGACAAGATTGGAACTGC+TGG 0.538859 6.1:-78552 None:intergenic
CCAGGAAAATCTTCTCAACT+GGG 0.540531 6.1:-78119 None:intergenic
CCTATACTTAGCAACTAACT+TGG 0.544461 6.1:-78529 None:intergenic
AAACCTATTCAACTCAAAGA+AGG 0.544786 6.1:+77302 MS.gene82768:CDS
TGGCCGAGGAGACTTGATCA+TGG 0.546979 6.1:-78214 None:intergenic
TCAGTTGCTTCGGCTCGGGT+TGG 0.547204 6.1:-78234 None:intergenic
ATCTGGGTAGCAGTCACCAC+AGG 0.548128 6.1:-78183 None:intergenic
CAATCTCCCTGGTGTTGTGG+TGG 0.549734 6.1:+77541 MS.gene82768:CDS
TTGTCCTGTGATCAAGAAGC+CGG 0.554022 6.1:+77464 MS.gene82768:CDS
TGGTATTTCCAGGCTTCAAG+TGG 0.557830 6.1:-77406 None:intergenic
ACTCTGCAATGTTGGGTGAG+AGG 0.560793 6.1:+77510 MS.gene82768:CDS
TGCGTACAGCAGATGATGCA+AGG 0.569708 6.1:-78449 None:intergenic
AGAGCAACAACGGCGTCACC+AGG 0.569748 6.1:-78768 None:intergenic
TCCAATGGACTCATAGGCAA+TGG 0.571271 6.1:-78423 None:intergenic
ACAGCGACATGTAACAGTAC+CGG 0.573187 6.1:-77483 None:intergenic
GGTTCCATCAAGAACTGCAT+TGG 0.573593 6.1:-78262 None:intergenic
GCATATTCAGTTGATGTCAA+AGG 0.575993 6.1:+77706 MS.gene82768:CDS
TGTTCCATCAGAGCATAGAA+TGG 0.578771 6.1:-77426 None:intergenic
TTGATTCCTATATTGGCAGA+AGG 0.579355 6.1:+78660 MS.gene82768:CDS
AGCAGACTTGAGATTATCGA+GGG 0.582018 6.1:-76489 None:intergenic
TGTCAATCTCCCTGGTGTTG+TGG 0.583872 6.1:+77538 MS.gene82768:CDS
TGTCGAACATAGACATTGAA+GGG 0.588964 6.1:+76300 MS.gene82768:CDS
GATCATGGATTCAAGCATCT+GGG 0.589960 6.1:-78199 None:intergenic
CTCATTGTCGTGCTGACACG+AGG 0.591073 6.1:+78495 MS.gene82768:CDS
TTATCCTTGTCAGTAAGAGT+GGG 0.593774 6.1:-77569 None:intergenic
AATCCATGATCAAGTCTCCT+CGG 0.597161 6.1:+78211 MS.gene82768:CDS
TGATCTTGTTAATGTCCGCA+AGG 0.600121 6.1:+77664 MS.gene82768:CDS
GGCCCGTGGTGATCTTGGAA+TGG 0.607650 6.1:+78092 MS.gene82768:CDS
AGCAGTTCCAATCTTGTCTG+TGG 0.608373 6.1:+78554 MS.gene82768:CDS
ACTACTGATTATGAAATCAA+GGG 0.609026 6.1:+77341 MS.gene82768:CDS
TGCCGTCATGCTCGACACCA+AGG 0.609600 6.1:+76535 MS.gene82768:CDS
GATGATGCAAGGCTTTCCAA+TGG 0.615392 6.1:-78438 None:intergenic
GAATATAGCACCATAATCAA+GGG 0.615983 6.1:-78379 None:intergenic
AAGGAAGATATTCTTGAATG+GGG 0.623075 6.1:+77590 MS.gene82768:CDS
TTGTCGTGCTGACACGAGGT+GGG 0.624494 6.1:+78499 MS.gene82768:CDS
ACCACTAAGCATGACACAGT+CGG 0.629348 6.1:-78283 None:intergenic
TTCAAGGTTGAGAATCAGGA+GGG 0.631704 6.1:+78021 MS.gene82768:intron
AGTACCGGCTTCTTGATCAC+AGG 0.634918 6.1:-77468 None:intergenic
TTTCAAGGTTGAGAATCAGG+AGG 0.635083 6.1:+78020 MS.gene82768:intron
TTAAGTCAGCAACACAAAAG+GGG 0.635960 6.1:+78736 MS.gene82768:CDS
GATGCGTTCATGGTGGCCCG+TGG 0.637855 6.1:+78078 MS.gene82768:CDS
AACTGAATATGCTTAGCATG+TGG 0.639472 6.1:-77695 None:intergenic
ATTGTCGTGCTGACACGAGG+TGG 0.639978 6.1:+78498 MS.gene82768:CDS
GGTCCAATCAAATGAGTCTG+TGG 0.640580 6.1:-78592 None:intergenic
TAGCAGACTTGAGATTATCG+AGG 0.648969 6.1:-76490 None:intergenic
AACCTTGGTGTCGAGCATGA+CGG 0.653974 6.1:-76537 None:intergenic
TGATTGGACCTGCAGTGATG+AGG 0.656525 6.1:+78605 MS.gene82768:CDS
TCTTCCCACTCTTACTGACA+AGG 0.660905 6.1:+77565 MS.gene82768:CDS
GCAGTGATGAGGCACCAGCA+AGG 0.676635 6.1:+78616 MS.gene82768:CDS
AATACGATGAAGAGCAACAA+CGG 0.684681 6.1:-78778 None:intergenic
TAATCTTGTTGGCAAGCCTG+TGG 0.690354 6.1:+78167 MS.gene82768:CDS
AGGCACAGCTTAATATACAG+AGG 0.690557 6.1:+78636 MS.gene82768:CDS
TTTCCATTCCAAGATCACCA+CGG 0.695446 6.1:-78095 None:intergenic
TTGAACCAACTACCAAACGA+TGG 0.710339 6.1:+76326 MS.gene82768:CDS
TGAACGCATCAGTCTCACGC+AGG 0.710992 6.1:-78065 None:intergenic
TGAGACTGATGCGTTCATGG+TGG 0.741662 6.1:+78071 MS.gene82768:CDS
GAAGATCCACCACAACACCA+GGG 0.757023 6.1:-77547 None:intergenic
CATGTGGCCCGAGAACCTTG+CGG 0.783590 6.1:-77679 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATTTTAATAATGATATAA+AGG + chr6.1:77753-77772 MS.gene82768:intron 10.0%
!!! TTATTATTTCAATTTTTGTA+AGG + chr6.1:76822-76841 MS.gene82768:intron 10.0%
!!! GTTTTTAAATAATGATTTTG+TGG + chr6.1:77990-78009 MS.gene82768:intron 15.0%
!! ATTTATAAGTGTAATCTTGT+TGG + chr6.1:78156-78175 MS.gene82768:CDS 20.0%
!! CAATCATATCAATATTGTTA+GGG - chr6.1:77619-77638 None:intergenic 20.0%
!! TTGAAAAGTTGTTAATTGAA+GGG - chr6.1:76772-76791 None:intergenic 20.0%
!! TTTGAAAAGTTGTTAATTGA+AGG - chr6.1:76773-76792 None:intergenic 20.0%
!!! AATCAATTTTGTGTTTAATC+AGG + chr6.1:76740-76759 MS.gene82768:intron 20.0%
! ACCAAAATTGTTTGTACTTT+GGG + chr6.1:76362-76381 MS.gene82768:CDS 25.0%
! ATAAGGAAGATATTCTTGAA+TGG + chr6.1:77588-77607 MS.gene82768:CDS 25.0%
! ATTCAAAAACTTCTTAAAGC+TGG + chr6.1:76410-76429 MS.gene82768:CDS 25.0%
! CATTAGAAGATATATCAACA+AGG - chr6.1:77841-77860 None:intergenic 25.0%
! CTATATTCAAAGAGATGATA+AGG + chr6.1:78394-78413 MS.gene82768:CDS 25.0%
! GATTTCATAATCAGTAGTTA+TGG - chr6.1:77339-77358 None:intergenic 25.0%
! GCAATCATATCAATATTGTT+AGG - chr6.1:77620-77639 None:intergenic 25.0%
! TAAGGAAGATATTCTTGAAT+GGG + chr6.1:77589-77608 MS.gene82768:CDS 25.0%
! TAGCAATAAAATAGTCTCTA+AGG - chr6.1:77131-77150 None:intergenic 25.0%
! TCATATTAAACAGACAATCA+TGG - chr6.1:77912-77931 None:intergenic 25.0%
!! AACTACTGATTATGAAATCA+AGG + chr6.1:77340-77359 MS.gene82768:CDS 25.0%
!! ACTACTGATTATGAAATCAA+GGG + chr6.1:77341-77360 MS.gene82768:CDS 25.0%
!! ATTCTTAACGTTTTCATGTA+CGG + chr6.1:77209-77228 MS.gene82768:intron 25.0%
!! TTTTGTGGAATTTCATTTCA+AGG + chr6.1:78005-78024 MS.gene82768:intron 25.0%
AAACCTATTCAACTCAAAGA+AGG + chr6.1:77302-77321 MS.gene82768:CDS 30.0%
AACACCACAAACAAATGAAA+TGG - chr6.1:77783-77802 None:intergenic 30.0%
AAGAGAATCATAACTGTACA+AGG - chr6.1:77158-77177 None:intergenic 30.0%
AAGGAAGATATTCTTGAATG+GGG + chr6.1:77590-77609 MS.gene82768:CDS 30.0%
AAGGACATGCAATTCATTAA+TGG - chr6.1:77822-77841 None:intergenic 30.0%
ATAGAAACAAGAACAAACCT+TGG - chr6.1:76555-76574 None:intergenic 30.0%
ATCACCATTTCATTTGTTTG+TGG + chr6.1:77776-77795 MS.gene82768:intron 30.0%
ATGCAATTCATTAATGGTGA+AGG - chr6.1:77816-77835 None:intergenic 30.0%
GAATATAGCACCATAATCAA+GGG - chr6.1:78382-78401 None:intergenic 30.0%
GACCAAAATTGTTTGTACTT+TGG + chr6.1:76361-76380 MS.gene82768:CDS 30.0%
TAACTCATTGATATCATCTC+CGG - chr6.1:77371-77390 None:intergenic 30.0%
TGAATATAGCACCATAATCA+AGG - chr6.1:78383-78402 None:intergenic 30.0%
TGCAATTCATTAATGGTGAA+GGG - chr6.1:77815-77834 None:intergenic 30.0%
! ACCCAAAGTACAAACAATTT+TGG - chr6.1:76366-76385 None:intergenic 30.0%
! TTGAACAAGTGATTTGTGTT+TGG + chr6.1:76671-76690 MS.gene82768:intron 30.0%
!! GTACAAACAATTTTGGTCTT+AGG - chr6.1:76359-76378 None:intergenic 30.0%
!!! TTTTTTGTCTGTAGACAAAG+TGG + chr6.1:77028-77047 MS.gene82768:intron 30.0%
AACTGAATATGCTTAGCATG+TGG - chr6.1:77698-77717 None:intergenic 35.0%
AATACCATTCTATGCTCTGA+TGG + chr6.1:77422-77441 MS.gene82768:CDS 35.0%
AATACGATGAAGAGCAACAA+CGG - chr6.1:78781-78800 None:intergenic 35.0%
AGAATCTACAGATGCAATTC+TGG + chr6.1:78704-78723 MS.gene82768:CDS 35.0%
ATGTCGAACATAGACATTGA+AGG + chr6.1:76299-76318 MS.gene82768:CDS 35.0%
ATTAAGCACGACTTCAGAAT+TGG - chr6.1:77969-77988 None:intergenic 35.0%
CAATTCATTAATGGTGAAGG+GGG - chr6.1:77813-77832 None:intergenic 35.0%
CATAGCAATAGCATAGCATA+GGG + chr6.1:76580-76599 MS.gene82768:intron 35.0%
CCAAGTTAGTTGCTAAGTAT+AGG + chr6.1:78529-78548 MS.gene82768:CDS 35.0%
CCTATACTTAGCAACTAACT+TGG - chr6.1:78532-78551 None:intergenic 35.0%
CTTAAGTCAGCAACACAAAA+GGG + chr6.1:78735-78754 MS.gene82768:CDS 35.0%
GCAATTCATTAATGGTGAAG+GGG - chr6.1:77814-77833 None:intergenic 35.0%
GCTCTTTCATTTGTTCGTAA+GGG + chr6.1:77638-77657 MS.gene82768:CDS 35.0%
TCATTTCAAGGTTGAGAATC+AGG + chr6.1:78017-78036 MS.gene82768:intron 35.0%
TCTTAAGTCAGCAACACAAA+AGG + chr6.1:78734-78753 MS.gene82768:CDS 35.0%
TGCTCTTTCATTTGTTCGTA+AGG + chr6.1:77637-77656 MS.gene82768:CDS 35.0%
TGGTACTCATGAATATCATC+AGG + chr6.1:76463-76482 MS.gene82768:CDS 35.0%
TGTCAAAACTCTGCAATGTT+GGG + chr6.1:77503-77522 MS.gene82768:CDS 35.0%
TGTCGAACATAGACATTGAA+GGG + chr6.1:76300-76319 MS.gene82768:CDS 35.0%
TTAAGTCAGCAACACAAAAG+GGG + chr6.1:78736-78755 MS.gene82768:CDS 35.0%
TTATCCTTGTCAGTAAGAGT+GGG - chr6.1:77572-77591 None:intergenic 35.0%
TTATGAAATCAAGGGAGATC+CGG + chr6.1:77349-77368 MS.gene82768:CDS 35.0%
TTGACATTGACAATTGAGCT+AGG - chr6.1:76848-76867 None:intergenic 35.0%
TTGATTCCTATATTGGCAGA+AGG + chr6.1:78660-78679 MS.gene82768:CDS 35.0%
! AAATCATCATTTTCTGTGCC+AGG - chr6.1:78140-78159 None:intergenic 35.0%
! GCATATTCAGTTGATGTCAA+AGG + chr6.1:77706-77725 MS.gene82768:CDS 35.0%
! GCTCGTTTCAATTTTTCACA+TGG + chr6.1:76443-76462 MS.gene82768:CDS 35.0%
!! AATTCTGAAGTCGTGCTTAA+TGG + chr6.1:77968-77987 MS.gene82768:intron 35.0%
!! ATGATTTTGACAGCAAGTTC+AGG - chr6.1:78330-78349 None:intergenic 35.0%
!! TACTGTACTGTGTTTTTGCA+GGG + chr6.1:77245-77264 MS.gene82768:intron 35.0%
!! TGAACTTGCTGTCAAAATCA+TGG + chr6.1:78329-78348 MS.gene82768:CDS 35.0%
!! TGATTTTGACAGCAAGTTCA+GGG - chr6.1:78329-78348 None:intergenic 35.0%
!! TGTCCTTCTTTGAGTTGAAT+AGG - chr6.1:77308-77327 None:intergenic 35.0%
!!! TTTTTTGTCTGTAGACAACG+CGG - chr6.1:76950-76969 None:intergenic 35.0%
AAGTCACACAAACGACTGTA+AGG + chr6.1:77073-77092 MS.gene82768:intron 40.0%
AAGTCTGCTATGCATTCTAC+TGG + chr6.1:76503-76522 MS.gene82768:CDS 40.0%
AATCCATGATCAAGTCTCCT+CGG + chr6.1:78211-78230 MS.gene82768:CDS 40.0%
AATCTTGTCTGTGGTAGTTC+CGG + chr6.1:78563-78582 MS.gene82768:CDS 40.0%
AATGAGTCTGTGGTCAAAAC+CGG - chr6.1:78585-78604 None:intergenic 40.0%
AGCAGACTTGAGATTATCGA+GGG - chr6.1:76492-76511 None:intergenic 40.0%
AGGAAAAATGTCAATCTCCC+TGG + chr6.1:77530-77549 MS.gene82768:CDS 40.0%
AGGCACAGCTTAATATACAG+AGG + chr6.1:78636-78655 MS.gene82768:CDS 40.0%
ATTGGCTACATCAGTTGCTT+CGG - chr6.1:78247-78266 None:intergenic 40.0%
CACTCTTACTGACAAGGATA+AGG + chr6.1:77571-77590 MS.gene82768:CDS 40.0%
CAGAGGCTTGATTCCTATAT+TGG + chr6.1:78653-78672 MS.gene82768:CDS 40.0%
CCAGGAAAATCTTCTCAACT+GGG - chr6.1:78122-78141 None:intergenic 40.0%
CCATCAATATCATGTTGCTG+AGG + chr6.1:76978-76997 MS.gene82768:intron 40.0%
CCTCAGCAACATGATATTGA+TGG - chr6.1:76981-77000 None:intergenic 40.0%
CTGTCAAAACTCTGCAATGT+TGG + chr6.1:77502-77521 MS.gene82768:CDS 40.0%
CTTATCCTTGTCAGTAAGAG+TGG - chr6.1:77573-77592 None:intergenic 40.0%
GAGCATAGAATGGTATTTCC+AGG - chr6.1:77419-77438 None:intergenic 40.0%
GATCATGGATTCAAGCATCT+GGG - chr6.1:78202-78221 None:intergenic 40.0%
GCAGAATCATCCCTTGATTA+TGG + chr6.1:78369-78388 MS.gene82768:CDS 40.0%
GCATAGCAATAGCATAGCAT+AGG + chr6.1:76579-76598 MS.gene82768:intron 40.0%
GTTGTTGCTCTTCATCGTAT+TGG + chr6.1:78780-78799 MS.gene82768:CDS 40.0%
TAGCAGACTTGAGATTATCG+AGG - chr6.1:76493-76512 None:intergenic 40.0%
TATATTAAGCTGTGCCTTGC+TGG - chr6.1:78633-78652 None:intergenic 40.0%
TCGTTTGTGTGACTTTCAAG+TGG - chr6.1:77068-77087 None:intergenic 40.0%
TGACCACAGACTCATTTGAT+TGG + chr6.1:78589-78608 MS.gene82768:CDS 40.0%
TGATCATGGATTCAAGCATC+TGG - chr6.1:78203-78222 None:intergenic 40.0%
TGATCTTGTTAATGTCCGCA+AGG + chr6.1:77664-77683 MS.gene82768:CDS 40.0%
TGTTCCATCAGAGCATAGAA+TGG - chr6.1:77429-77448 None:intergenic 40.0%
TTCAAGGTTGAGAATCAGGA+GGG + chr6.1:78021-78040 MS.gene82768:intron 40.0%
TTGAACCAACTACCAAACGA+TGG + chr6.1:76326-76345 MS.gene82768:CDS 40.0%
TTTCAAGGTTGAGAATCAGG+AGG + chr6.1:78020-78039 MS.gene82768:intron 40.0%
TTTCCATTCCAAGATCACCA+CGG - chr6.1:78098-78117 None:intergenic 40.0%
!! ACTGTACTGTGTTTTTGCAG+GGG + chr6.1:77246-77265 MS.gene82768:intron 40.0%
!! CTACTGTACTGTGTTTTTGC+AGG + chr6.1:77244-77263 MS.gene82768:intron 40.0%
AAGGTTTGACACCTTCACCT+GGG - chr6.1:77112-77131 None:intergenic 45.0%
ACAAACGACTGTAAGGTCCT+AGG + chr6.1:77080-77099 MS.gene82768:intron 45.0%
ACAGACAAGATTGGAACTGC+TGG - chr6.1:78555-78574 None:intergenic 45.0%
ACAGCGACATGTAACAGTAC+CGG - chr6.1:77486-77505 None:intergenic 45.0%
ACCACTAAGCATGACACAGT+CGG - chr6.1:78286-78305 None:intergenic 45.0%
AGCAGTTCCAATCTTGTCTG+TGG + chr6.1:78554-78573 MS.gene82768:CDS 45.0%
ATCTCAACAGATCTCGAAGC+AGG - chr6.1:76389-76408 None:intergenic 45.0%
GATGATGCAAGGCTTTCCAA+TGG - chr6.1:78441-78460 None:intergenic 45.0%
GCAGATCCTTCTGCCAATAT+AGG - chr6.1:78669-78688 None:intergenic 45.0%
GCCAGGAAAATCTTCTCAAC+TGG - chr6.1:78123-78142 None:intergenic 45.0%
GGAACTACCACAGACAAGAT+TGG - chr6.1:78564-78583 None:intergenic 45.0%
GGTCCAATCAAATGAGTCTG+TGG - chr6.1:78595-78614 None:intergenic 45.0%
GGTTCCATCAAGAACTGCAT+TGG - chr6.1:78265-78284 None:intergenic 45.0%
GTAGCCAATGCAGTTCTTGA+TGG + chr6.1:78258-78277 MS.gene82768:CDS 45.0%
GTTAATGTCCGCAAGGTTCT+CGG + chr6.1:77671-77690 MS.gene82768:CDS 45.0%
TAAGGTTTGACACCTTCACC+TGG - chr6.1:77113-77132 None:intergenic 45.0%
TAATCTTGTTGGCAAGCCTG+TGG + chr6.1:78167-78186 MS.gene82768:CDS 45.0%
TAGACAAGACATCCTCACCT+GGG + chr6.1:76874-76893 MS.gene82768:intron 45.0%
TCCATTGCCTATGAGTCCAT+TGG + chr6.1:78422-78441 MS.gene82768:CDS 45.0%
TCTTCCCACTCTTACTGACA+AGG + chr6.1:77565-77584 MS.gene82768:CDS 45.0%
TGGTATTTCCAGGCTTCAAG+TGG - chr6.1:77409-77428 None:intergenic 45.0%
TTAATGTCCGCAAGGTTCTC+GGG + chr6.1:77672-77691 MS.gene82768:CDS 45.0%
TTAGACAAGACATCCTCACC+TGG + chr6.1:76873-76892 MS.gene82768:intron 45.0%
TTAGGAACACGACCATCGTT+TGG - chr6.1:76341-76360 None:intergenic 45.0%
TTCCATTCCAAGATCACCAC+GGG - chr6.1:78097-78116 None:intergenic 45.0%
TTGTCCTGTGATCAAGAAGC+CGG + chr6.1:77464-77483 MS.gene82768:CDS 45.0%
! AGGCTTTCCAATGGACTCAT+AGG - chr6.1:78432-78451 None:intergenic 45.0%
! CAAAAGGGGCTTTGTAAACC+TGG + chr6.1:78750-78769 MS.gene82768:CDS 45.0%
! CCCAGTTGAGAAGATTTTCC+TGG + chr6.1:78119-78138 MS.gene82768:CDS 45.0%
! TCCAATGGACTCATAGGCAA+TGG - chr6.1:78426-78445 None:intergenic 45.0%
ACCGACTGTGTCATGCTTAG+TGG + chr6.1:78282-78301 MS.gene82768:CDS 50.0%
ACGCACAACTGCGAGATTGT+GGG - chr6.1:76906-76925 None:intergenic 50.0%
ACTCTGCAATGTTGGGTGAG+AGG + chr6.1:77510-77529 MS.gene82768:CDS 50.0%
AGAAAGCCAGTCCGAATCTC+AGG - chr6.1:77272-77291 None:intergenic 50.0%
CACGACAATGAGTTTCGCTC+TGG - chr6.1:78487-78506 None:intergenic 50.0%
CTACATCAGTTGCTTCGGCT+CGG - chr6.1:78242-78261 None:intergenic 50.0%
GAAGATCCACCACAACACCA+GGG - chr6.1:77550-77569 None:intergenic 50.0%
GAGATTGTGGGTACAAACCC+AGG - chr6.1:76894-76913 None:intergenic 50.0%
TACATCAGTTGCTTCGGCTC+GGG - chr6.1:78241-78260 None:intergenic 50.0%
TGAGACTGATGCGTTCATGG+TGG + chr6.1:78071-78090 MS.gene82768:CDS 50.0%
TGATTGGACCTGCAGTGATG+AGG + chr6.1:78605-78624 MS.gene82768:CDS 50.0%
TGCGTACAGCAGATGATGCA+AGG - chr6.1:78452-78471 None:intergenic 50.0%
TGCTGTACGCACAGCCAATA+AGG + chr6.1:78461-78480 MS.gene82768:CDS 50.0%
TTTCCAGGCTTCAAGTGGAC+AGG - chr6.1:77404-77423 None:intergenic 50.0%
! AAGGTCCTAGGTTCTAACCC+AGG + chr6.1:77092-77111 MS.gene82768:intron 50.0%
! CACGACCATCGTTTGGTAGT+TGG - chr6.1:76334-76353 None:intergenic 50.0%
! CTAGGTTCTAACCCAGGTGA+AGG + chr6.1:77098-77117 MS.gene82768:intron 50.0%
! CTTCACCTGGGTTAGAACCT+AGG - chr6.1:77100-77119 None:intergenic 50.0%
! TGTCAATCTCCCTGGTGTTG+TGG + chr6.1:77538-77557 MS.gene82768:CDS 50.0%
! TTTTGCAGGGGCCTGAGATT+CGG + chr6.1:77258-77277 MS.gene82768:intron 50.0%
!! AACCTTGGTGTCGAGCATGA+CGG - chr6.1:76540-76559 None:intergenic 50.0%
!! AGTACCGGCTTCTTGATCAC+AGG - chr6.1:77471-77490 None:intergenic 50.0%
!! CGGACTGGCTTTCTGAAAGA+TGG + chr6.1:77278-77297 MS.gene82768:CDS 50.0%
!! CTTAGTGGTGAAAGTGCTGC+TGG + chr6.1:78297-78316 MS.gene82768:CDS 50.0%
!! GAGTTTCGCTCTGGCCTTAT+TGG - chr6.1:78478-78497 None:intergenic 50.0%
ATCTGGGTAGCAGTCACCAC+AGG - chr6.1:78186-78205 None:intergenic 55.0%
CACGCACAACTGCGAGATTG+TGG - chr6.1:76907-76926 None:intergenic 55.0%
GCGTGAGACTGATGCGTTCA+TGG + chr6.1:78068-78087 MS.gene82768:CDS 55.0%
GGAAGATCCACCACAACACC+AGG - chr6.1:77551-77570 None:intergenic 55.0%
TGAACGCATCAGTCTCACGC+AGG - chr6.1:78068-78087 None:intergenic 55.0%
TGGCCGAGGAGACTTGATCA+TGG - chr6.1:78217-78236 None:intergenic 55.0%
TGTGGGTACAAACCCAGGTG+AGG - chr6.1:76889-76908 None:intergenic 55.0%
! ATTGTCGTGCTGACACGAGG+TGG + chr6.1:78498-78517 MS.gene82768:CDS 55.0%
! CTCATTGTCGTGCTGACACG+AGG + chr6.1:78495-78514 MS.gene82768:CDS 55.0%
! TTGTCGTGCTGACACGAGGT+GGG + chr6.1:78499-78518 MS.gene82768:CDS 55.0%
!! CAATCTCCCTGGTGTTGTGG+TGG + chr6.1:77541-77560 MS.gene82768:CDS 55.0%
AGAGCAACAACGGCGTCACC+AGG - chr6.1:78771-78790 None:intergenic 60.0%
ATGGTGGCCCGTGGTGATCT+TGG + chr6.1:78087-78106 MS.gene82768:CDS 60.0%
CATGTGGCCCGAGAACCTTG+CGG - chr6.1:77682-77701 None:intergenic 60.0%
CTGCCTGTCCACTTGAAGCC+TGG + chr6.1:77398-77417 MS.gene82768:CDS 60.0%
GGCCCGTGGTGATCTTGGAA+TGG + chr6.1:78092-78111 MS.gene82768:CDS 60.0%
TGCCGTCATGCTCGACACCA+AGG + chr6.1:76535-76554 MS.gene82768:CDS 60.0%
TGCTGGTGCCTCATCACTGC+AGG - chr6.1:78616-78635 None:intergenic 60.0%
! GCAGTGATGAGGCACCAGCA+AGG + chr6.1:78616-78635 MS.gene82768:CDS 60.0%
!! TCAGTTGCTTCGGCTCGGGT+TGG - chr6.1:78237-78256 None:intergenic 60.0%
CAGGGGCCTGAGATTCGGAC+TGG + chr6.1:77263-77282 MS.gene82768:intron 65.0%
GATGCGTTCATGGTGGCCCG+TGG + chr6.1:78078-78097 MS.gene82768:CDS 65.0%
!! GCTTCGGCTCGGGTTGGCCG+AGG - chr6.1:78231-78250 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 76299 78839 76299 ID=MS.gene82768
chr6.1 mRNA 76299 78839 76299 ID=MS.gene82768.t1;Parent=MS.gene82768
chr6.1 exon 76299 76556 76299 ID=MS.gene82768.t1.exon1;Parent=MS.gene82768.t1
chr6.1 CDS 76299 76556 76299 ID=cds.MS.gene82768.t1;Parent=MS.gene82768.t1
chr6.1 exon 77266 77727 77266 ID=MS.gene82768.t1.exon2;Parent=MS.gene82768.t1
chr6.1 CDS 77266 77727 77266 ID=cds.MS.gene82768.t1;Parent=MS.gene82768.t1
chr6.1 exon 78027 78839 78027 ID=MS.gene82768.t1.exon3;Parent=MS.gene82768.t1
chr6.1 CDS 78027 78839 78027 ID=cds.MS.gene82768.t1;Parent=MS.gene82768.t1
Gene Sequence

>MS.gene82768

ATGTCGAACATAGACATTGAAGGGATCTTGAACCAACTACCAAACGATGGTCGTGTTCCTAAGACCAAAATTGTTTGTACTTTGGGTCCTGCTTCGAGATCTGTTGAGATGATTCAAAAACTTCTTAAAGCTGGCATGAATGTTGCTCGTTTCAATTTTTCACATGGTACTCATGAATATCATCAGGAAACCCTCGATAATCTCAAGTCTGCTATGCATTCTACTGGTATTCTCTGTGCCGTCATGCTCGACACCAAGGTTTGTTCTTGTTTCTATACATGCATAGCAATAGCATAGCATAGGGATCACTATATATGTTCTTGCTTGCATTGAATTTGATTTTGAGTGAATTGATTTTTGTCTAAAACTCAGTTGAACAAGTGATTTGTGTTTGGATACATTTATATAAACAATCAATTTGAGTGTAAAAATCAATTCTAGAATCAATTTTGTGTTTAATCAGGATTGTGCCCTTCAATTAACAACTTTTCAAAGCTAGTACTCAACATTTTTTTATGCTTTTTTATTATTTCAATTTTTGTAAGGCCTAGCTCAATTGTCAATGTCAATATTGTTAGACAAGACATCCTCACCTGGGTTTGTACCCACAATCTCGCAGTTGTGCGTGTGATTTTCACGTTATGCTTGCCGCGTTGTCTACAGACAAAAAAATATTATTCCATCAATATCATGTTGCTGAGGATGATGTTTTATTGCTGTTTTTTTTTTTTTTTTGTCTGTAGACAAAGTGGTAATTTAGTGATTACCACTTGAAAGTCACACAAACGACTGTAAGGTCCTAGGTTCTAACCCAGGTGAAGGTGTCAAACCTTAGAGACTATTTTATTGCTATTTTCCTTGTACAGTTATGATTCTCTTATATGTAGTGATGAATTTCTTTCTTAATGAAATTCTTAACGTTTTCATGTACGGTACATATGCTCTCTACTGTACTGTGTTTTTGCAGGGGCCTGAGATTCGGACTGGCTTTCTGAAAGATGGAAAACCTATTCAACTCAAAGAAGGACAAGAAATCACCATAACTACTGATTATGAAATCAAGGGAGATCCGGAGATGATATCAATGAGTTACAAGAAACTGCCTGTCCACTTGAAGCCTGGAAATACCATTCTATGCTCTGATGGAACAATCAGTCTCACTGTTTTGTCCTGTGATCAAGAAGCCGGTACTGTTACATGTCGCTGTCAAAACTCTGCAATGTTGGGTGAGAGGAAAAATGTCAATCTCCCTGGTGTTGTGGTGGATCTTCCCACTCTTACTGACAAGGATAAGGAAGATATTCTTGAATGGGGTGTCCCTAACAATATTGATATGATTGCTCTTTCATTTGTTCGTAAGGGCTCTGATCTTGTTAATGTCCGCAAGGTTCTCGGGCCACATGCTAAGCATATTCAGTTGATGTCAAAGGTAGAATTTTAATTGCATTCTTCTGCAATTTTAATAATGATATAAAGGATCACCATTTCATTTGTTTGTGGTGTTAATTTTTCCCCCTTCACCATTAATGAATTGCATGTCCTTGTTGATATATCTTCTAATGCATCTTTAAGTTGAATGTAATTCATCATTTTTCTCTTCAACACAAATTCCATGATTGTCTGTTTAATATGAACAAGTACTAACTTGTGTGTTTGTGTTTCTTACACCAATTCTGAAGTCGTGCTTAATGGTTTTTAAATAATGATTTTGTGGAATTTCATTTCAAGGTTGAGAATCAGGAGGGAGTCATGAACTTTGATGAGATCCTGCGTGAGACTGATGCGTTCATGGTGGCCCGTGGTGATCTTGGAATGGAAATCCCAGTTGAGAAGATTTTCCTGGCACAGAAAATGATGATTTATAAGTGTAATCTTGTTGGCAAGCCTGTGGTGACTGCTACCCAGATGCTTGAATCCATGATCAAGTCTCCTCGGCCAACCCGAGCCGAAGCAACTGATGTAGCCAATGCAGTTCTTGATGGAACCGACTGTGTCATGCTTAGTGGTGAAAGTGCTGCTGGAGCATACCCTGAACTTGCTGTCAAAATCATGGCTCGCATTTGTGTTGAAGCAGAATCATCCCTTGATTATGGTGCTATATTCAAAGAGATGATAAGGTCTACTCCATTGCCTATGAGTCCATTGGAAAGCCTTGCATCATCTGCTGTACGCACAGCCAATAAGGCCAGAGCGAAACTCATTGTCGTGCTGACACGAGGTGGGAGTACTGCCAAGTTAGTTGCTAAGTATAGGCCAGCAGTTCCAATCTTGTCTGTGGTAGTTCCGGTTTTGACCACAGACTCATTTGATTGGACCTGCAGTGATGAGGCACCAGCAAGGCACAGCTTAATATACAGAGGCTTGATTCCTATATTGGCAGAAGGATCTGCAAGAGCTACTGATGCAGAATCTACAGATGCAATTCTGGAAGCTTCTCTTAAGTCAGCAACACAAAAGGGGCTTTGTAAACCTGGTGACGCCGTTGTTGCTCTTCATCGTATTGGAGCTGCTTCTGTTATAAAGATCTGCATAGTGAAGTGA
Protein sequence

>MS.gene82768.t1

MSNIDIEGILNQLPNDGRVPKTKIVCTLGPASRSVEMIQKLLKAGMNVARFNFSHGTHEYHQETLDNLKSAMHSTGILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEITITTDYEIKGDPEMISMSYKKLPVHLKPGNTILCSDGTISLTVLSCDQEAGTVTCRCQNSAMLGERKNVNLPGVVVDLPTLTDKDKEDILEWGVPNNIDMIALSFVRKGSDLVNVRKVLGPHAKHIQLMSKVENQEGVMNFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLVGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPELAVKIMARICVEAESSLDYGAIFKEMIRSTPLPMSPLESLASSAVRTANKARAKLIVVLTRGGSTAKLVAKYRPAVPILSVVVPVLTTDSFDWTCSDEAPARHSLIYRGLIPILAEGSARATDAESTDAILEASLKSATQKGLCKPGDAVVALHRIGAASVIKICIVK