AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01229

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01229.t1 MTR_2g009800 98.559 555 8 0 1 555 1 555 0.0 1126
MS.gene01229.t1 MTR_2g101620 91.351 555 48 0 1 555 1 555 0.0 1050
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01229.t1 AT5G14050 62.832 565 181 11 1 555 1 546 0.0 681

Find 114 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTATTCACCTCCTGAATT+TGG 0.168421 2.4:-73373838 MS.gene01229:CDS
GACTGGGCGCAGCTGGATTC+AGG 0.199184 2.4:-73373502 MS.gene01229:CDS
ATGGAACTGAACTGAATTAA+TGG 0.221155 2.4:+73373279 None:intergenic
GAGGATGAGGATTTGATATC+TGG 0.277710 2.4:-73373580 MS.gene01229:CDS
TAGCCAAAGTCAACAGATTA+AGG 0.291410 2.4:-73373618 MS.gene01229:CDS
CTGAGAAGTCAGCATCTTCT+TGG 0.323321 2.4:+73373719 None:intergenic
AAAGCTATCATTTGAGAATC+AGG 0.336697 2.4:+73372989 None:intergenic
AAGGAAGATACTTTCAATCT+TGG 0.340340 2.4:+73373180 None:intergenic
GAAGAGTATTGAAGTGTTTG+AGG 0.342182 2.4:-73373017 MS.gene01229:CDS
AGATTATCAATGGTCTTGAT+AGG 0.358024 2.4:+73372650 None:intergenic
TACTTCAGGCATGGTGATAA+TGG 0.362722 2.4:+73372393 None:intergenic
TCAACAGATTAAGGAAGTTA+AGG 0.371537 2.4:-73373609 MS.gene01229:CDS
AGGAACTGACTGGGCGCAGC+TGG 0.391668 2.4:-73373509 MS.gene01229:CDS
GTTAAGGAAGGAGGAGGATG+AGG 0.392451 2.4:-73373593 MS.gene01229:CDS
TGGGCTAAAATCAATACAAC+GGG 0.399262 2.4:+73372475 None:intergenic
TGGAACTGAACTGAATTAAT+GGG 0.402013 2.4:+73373280 None:intergenic
CTTCCTTAATCTGTTGACTT+TGG 0.407960 2.4:+73373615 None:intergenic
GGGTCTCAGGTGATAATATC+AGG 0.411556 2.4:-73373118 MS.gene01229:CDS
TTTCTTGCCCGATGGGTCTC+AGG 0.414581 2.4:-73373131 MS.gene01229:CDS
ATGGCTGTAGGAAATGCTTC+AGG 0.420095 2.4:-73372440 MS.gene01229:CDS
TTATAGTCTTGATTTAGTTA+AGG 0.420893 2.4:-73373080 MS.gene01229:CDS
TTCGAATGGGGCAATCTTCA+AGG 0.424695 2.4:+73373161 None:intergenic
GGTCAGCTGCTTCTTACTGC+TGG 0.428798 2.4:-73373250 MS.gene01229:CDS
TCAGTTCAGTTCCATCGAAA+TGG 0.438541 2.4:-73373271 MS.gene01229:CDS
AAATACTAGCAATCTGTTCA+AGG 0.442805 2.4:-73372586 MS.gene01229:CDS
TTCAGTATTCAGATTATCAA+TGG 0.445525 2.4:+73372640 None:intergenic
AATATACAAGATCCCGCTAA+TGG 0.453091 2.4:-73373304 MS.gene01229:CDS
TACAATAGCGAGGAATTTCT+CGG 0.460074 2.4:-73372686 MS.gene01229:CDS
GAGCCGAGGTTACGAGGATC+TGG 0.461776 2.4:-73373419 MS.gene01229:CDS
GTAGATGAAGGCTGTTTAAA+CGG 0.466326 2.4:-73372779 MS.gene01229:CDS
TCACCTCCTGAATTTGGAAA+GGG 0.467907 2.4:-73373832 MS.gene01229:CDS
AAGGCTAGAGTTGATAAGAT+TGG 0.470242 2.4:-73373061 MS.gene01229:CDS
AGCTCAACCGAGGAACTGAC+TGG 0.472580 2.4:-73373519 MS.gene01229:CDS
TGATGGTCATATTTACCATT+GGG 0.474225 2.4:-73372834 MS.gene01229:CDS
GATAAGATTGGTCCGCTGTT+GGG 0.475592 2.4:-73373049 MS.gene01229:CDS
ACTGGGCGCAGCTGGATTCA+GGG 0.478998 2.4:-73373501 MS.gene01229:CDS
AAGCATCTTTCTTGCCCGAT+GGG 0.479466 2.4:-73373138 MS.gene01229:CDS
TTTGCAGCTGGTTCAGCTAG+TGG 0.484592 2.4:-73372722 MS.gene01229:CDS
GTGATGGTCATATTTACCAT+TGG 0.489547 2.4:-73372835 MS.gene01229:CDS
AAGGAAGTTAAGGAAGGAGG+AGG 0.489554 2.4:-73373599 MS.gene01229:CDS
AAAGCCGGTGTGGGTGGATG+AGG 0.489666 2.4:-73373665 MS.gene01229:CDS
CAGAGTATGTGTCAAGATTG+AGG 0.492768 2.4:-73373555 MS.gene01229:CDS
ACAAAGTCGAAGCAATTAGT+TGG 0.498298 2.4:-73372932 MS.gene01229:CDS
ACAATAGCGAGGAATTTCTC+GGG 0.503579 2.4:-73372685 MS.gene01229:CDS
TTCTTACTGCTGGATTAGAC+CGG 0.504668 2.4:-73373240 MS.gene01229:CDS
AGAGTATGTGTCAAGATTGA+GGG 0.513752 2.4:-73373554 MS.gene01229:CDS
GAATTAATGGGACCATTAGC+GGG 0.514356 2.4:+73373292 None:intergenic
TGATAAGATTGGTCCGCTGT+TGG 0.514998 2.4:-73373050 MS.gene01229:CDS
GATTGGTCCGCTGTTGGGTA+GGG 0.516250 2.4:-73373044 MS.gene01229:CDS
GGATGCCGCGGCTTCTGCAA+AGG 0.518753 2.4:-73373794 MS.gene01229:CDS
GCTGCGCCCAGTCAGTTCCT+CGG 0.520974 2.4:+73373512 None:intergenic
CTCTTTGTACTTCTCGAGTA+GGG 0.521391 2.4:-73372751 MS.gene01229:CDS
TGAATTAATGGGACCATTAG+CGG 0.525771 2.4:+73373291 None:intergenic
ATTAAGGAAGTTAAGGAAGG+AGG 0.530708 2.4:-73373602 MS.gene01229:CDS
GGTGGATGAGGAAGAAGAAA+AGG 0.532095 2.4:-73373653 MS.gene01229:CDS
TTCGAGTATTCAAGATGTCC+TGG 0.533881 2.4:+73373340 None:intergenic
AAAGGAAATGAGGTTGAAGA+TGG 0.535620 2.4:-73373982 MS.gene01229:CDS
GCTCTTTGTACTTCTCGAGT+AGG 0.536198 2.4:-73372752 MS.gene01229:CDS
AGATTGGTCCGCTGTTGGGT+AGG 0.539038 2.4:-73373045 MS.gene01229:CDS
TTCACCTCCTGAATTTGGAA+AGG 0.542160 2.4:-73373833 MS.gene01229:CDS
ATTATCACCTGAGACCCATC+GGG 0.550131 2.4:+73373124 None:intergenic
AGATGGAAAGGAAGAACAGT+TGG 0.550771 2.4:-73373914 MS.gene01229:CDS
TTGAGCTACTCTTCACAACC+AGG 0.551752 2.4:+73373371 None:intergenic
CAATACAACGGGGATAATGC+AGG 0.553209 2.4:+73372486 None:intergenic
AAAGAGGAAGCTAGATGGAA+AGG 0.553316 2.4:-73373926 MS.gene01229:CDS
TGATAATCTGAATACTGAAG+TGG 0.554817 2.4:-73372636 MS.gene01229:CDS
TTGTTAAGCGCTGGTGGTGA+TGG 0.557212 2.4:-73372851 MS.gene01229:CDS
AAAGCATCTTTCTTGCCCGA+TGG 0.557386 2.4:-73373139 MS.gene01229:CDS
TATTATCACCTGAGACCCAT+CGG 0.558404 2.4:+73373123 None:intergenic
AAATGAGGTTGAAGATGGGA+AGG 0.558756 2.4:-73373977 MS.gene01229:CDS
AAGGAAATGAGGTTGAAGAT+GGG 0.562237 2.4:-73373981 MS.gene01229:CDS
AGTAGCTCAAAATTGTTGCC+AGG 0.564255 2.4:-73373358 MS.gene01229:CDS
GGAGTTTGATGTTGATACTG+AGG 0.566399 2.4:-73373956 MS.gene01229:CDS
CAGATTAAGGAAGTTAAGGA+AGG 0.571312 2.4:-73373605 MS.gene01229:CDS
ACCTCCTGAATTTGGAAAGG+GGG 0.574743 2.4:-73373830 MS.gene01229:CDS
TGCTGTGAGCCGAGGTTACG+AGG 0.576924 2.4:-73373425 MS.gene01229:CDS
CATCCAGATCCTCGTAACCT+CGG 0.587962 2.4:+73373416 None:intergenic
AATACTAGCAATCTGTTCAA+GGG 0.593971 2.4:-73372585 MS.gene01229:CDS
GGAAGTTGTGAAAGGAAATG+AGG 0.594864 2.4:-73373992 MS.gene01229:CDS
TTTCTGATCTACTTCAGGCA+TGG 0.600646 2.4:+73372384 None:intergenic
ATACACAAAGGTGTAGATGA+AGG 0.605326 2.4:-73372791 MS.gene01229:CDS
CTTCAGTAAAAGCTAATGAA+CGG 0.605560 2.4:+73372885 None:intergenic
TGTGAATATTTACAATAGCG+AGG 0.608077 2.4:-73372696 MS.gene01229:CDS
GAAGCAGCTGACCATTTCGA+TGG 0.609256 2.4:+73373260 None:intergenic
AAAAGGGAGGAAGTTGTGAA+AGG 0.616065 2.4:-73374000 MS.gene01229:CDS
AATAAAGCTGCTGTGAGCCG+AGG 0.616455 2.4:-73373433 MS.gene01229:CDS
AGAAGCCTTTGCAGAAGCCG+CGG 0.617253 2.4:+73373789 None:intergenic
TCTCAGAACAAATGAAGACC+TGG 0.621951 2.4:-73373389 MS.gene01229:CDS
ACCATTGGGATCTGAGAACA+AGG 0.623692 2.4:-73372820 MS.gene01229:CDS
CTGGGCTAAAATCAATACAA+CGG 0.628701 2.4:+73372474 None:intergenic
ACAGCCATAAAACCACCACC+TGG 0.630227 2.4:+73372455 None:intergenic
TGATGATGAGGTGGATGCCG+CGG 0.633167 2.4:-73373806 MS.gene01229:CDS
AAAGGAAGAACAGTTGGCAA+AGG 0.637301 2.4:-73373908 MS.gene01229:CDS
CACCTCCTGAATTTGGAAAG+GGG 0.637398 2.4:-73373831 MS.gene01229:CDS
GCTCAACCGAGGAACTGACT+GGG 0.639373 2.4:-73373518 MS.gene01229:CDS
CCAGTTAGAGAAAACAGTGT+AGG 0.639938 2.4:+73372526 None:intergenic
GTTGGTACTCTGAAGATGAA+CGG 0.640176 2.4:-73372914 MS.gene01229:CDS
TTAGAGAAAACAGTGTAGGA+TGG 0.640295 2.4:+73372530 None:intergenic
CAATAGCGAGGAATTTCTCG+GGG 0.640898 2.4:-73372684 MS.gene01229:CDS
GAAAAGAAAGCCGGTGTGGG+TGG 0.643363 2.4:-73373671 MS.gene01229:CDS
TGCAGGCTCGCATTTGAACG+CGG 0.645712 2.4:+73372503 None:intergenic
TCTTCCTCATCCACCCACAC+CGG 0.648855 2.4:+73373661 None:intergenic
CTTTGAAAAGAAAGCCGGTG+TGG 0.649038 2.4:-73373675 MS.gene01229:CDS
TTCTCTTCCCTACCCAACAG+CGG 0.649632 2.4:+73373037 None:intergenic
TGATGCTTTGAAAAGAAAGC+CGG 0.651576 2.4:-73373680 MS.gene01229:CDS
CAGCCATAAAACCACCACCT+GGG 0.668450 2.4:+73372456 None:intergenic
CATCTTCTTGGTAAACAGTG+AGG 0.681529 2.4:+73373731 None:intergenic
TGAGGAGACTATCTGCAGAA+CGG 0.693456 2.4:+73373749 None:intergenic
TTTGAAAAGAAAGCCGGTGT+GGG 0.693980 2.4:-73373674 MS.gene01229:CDS
ACAAGGACTTGTATACACAA+AGG 0.694652 2.4:-73372803 MS.gene01229:CDS
CAGCATGCAAAGCTCAACCG+AGG 0.712589 2.4:-73373529 MS.gene01229:CDS
TCCTTGTTCTCAGATCCCAA+TGG 0.719029 2.4:+73372819 None:intergenic
AATAGCGAGGAATTTCTCGG+GGG 0.758971 2.4:-73372683 MS.gene01229:CDS
GGGCTAAAATCAATACAACG+GGG 0.814054 2.4:+73372476 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTAAATTTTTTCAAATTGA+TGG - chr2.4:73373225-73373244 MS.gene01229:CDS 15.0%
!! TTTGAAAAAATTTAAGCTTC+CGG + chr2.4:73373221-73373240 None:intergenic 20.0%
!!! TTATAGTCTTGATTTAGTTA+AGG - chr2.4:73373359-73373378 MS.gene01229:CDS 20.0%
! TTCAGTATTCAGATTATCAA+TGG + chr2.4:73373802-73373821 None:intergenic 25.0%
!! CAATGAAGGTTATATTTTAC+TGG - chr2.4:73373479-73373498 MS.gene01229:CDS 25.0%
!!! TTTTTCAAATTGATGGCAAA+CGG - chr2.4:73373232-73373251 MS.gene01229:CDS 25.0%
AAAGCTATCATTTGAGAATC+AGG + chr2.4:73373453-73373472 None:intergenic 30.0%
AAATACTAGCAATCTGTTCA+AGG - chr2.4:73373853-73373872 MS.gene01229:CDS 30.0%
AAGGAAGATACTTTCAATCT+TGG + chr2.4:73373262-73373281 None:intergenic 30.0%
AATACTAGCAATCTGTTCAA+GGG - chr2.4:73373854-73373873 MS.gene01229:CDS 30.0%
ATGGAACTGAACTGAATTAA+TGG + chr2.4:73373163-73373182 None:intergenic 30.0%
CTTCAGTAAAAGCTAATGAA+CGG + chr2.4:73373557-73373576 None:intergenic 30.0%
TCAACAGATTAAGGAAGTTA+AGG - chr2.4:73372830-73372849 MS.gene01229:CDS 30.0%
TGATAATCTGAATACTGAAG+TGG - chr2.4:73373803-73373822 MS.gene01229:CDS 30.0%
TGATGGTCATATTTACCATT+GGG - chr2.4:73373605-73373624 MS.gene01229:CDS 30.0%
TGGAACTGAACTGAATTAAT+GGG + chr2.4:73373162-73373181 None:intergenic 30.0%
TGTGAATATTTACAATAGCG+AGG - chr2.4:73373743-73373762 MS.gene01229:CDS 30.0%
! AAGAAAGATGCTTTTCGAAT+GGG + chr2.4:73373294-73373313 None:intergenic 30.0%
! AGATTATCAATGGTCTTGAT+AGG + chr2.4:73373792-73373811 None:intergenic 30.0%
! CTCTAAAAATGAGACGAAAA+GGG - chr2.4:73372423-73372442 MS.gene01229:CDS 30.0%
! TCTCAAATGATAGCTTTTGT+GGG - chr2.4:73373456-73373475 MS.gene01229:CDS 30.0%
! TTCTCAAATGATAGCTTTTG+TGG - chr2.4:73373455-73373474 MS.gene01229:CDS 30.0%
!!! TCATTAGCTTTTACTGAAGA+TGG - chr2.4:73373558-73373577 MS.gene01229:CDS 30.0%
AAAGGAAATGAGGTTGAAGA+TGG - chr2.4:73372457-73372476 MS.gene01229:CDS 35.0%
AAGGAAATGAGGTTGAAGAT+GGG - chr2.4:73372458-73372477 MS.gene01229:CDS 35.0%
AATATACAAGATCCCGCTAA+TGG - chr2.4:73373135-73373154 MS.gene01229:CDS 35.0%
ACAAAGTCGAAGCAATTAGT+TGG - chr2.4:73373507-73373526 MS.gene01229:CDS 35.0%
ACAAGGACTTGTATACACAA+AGG - chr2.4:73373636-73373655 MS.gene01229:CDS 35.0%
AGAGTATGTGTCAAGATTGA+GGG - chr2.4:73372885-73372904 MS.gene01229:CDS 35.0%
ATACTGAGGAAACGAAAAAG+AGG - chr2.4:73372497-73372516 MS.gene01229:CDS 35.0%
ATTAAGGAAGTTAAGGAAGG+AGG - chr2.4:73372837-73372856 MS.gene01229:CDS 35.0%
CAGATTAAGGAAGTTAAGGA+AGG - chr2.4:73372834-73372853 MS.gene01229:CDS 35.0%
CTGGGCTAAAATCAATACAA+CGG + chr2.4:73373968-73373987 None:intergenic 35.0%
GTAGATGAAGGCTGTTTAAA+CGG - chr2.4:73373660-73373679 MS.gene01229:CDS 35.0%
GTGATGGTCATATTTACCAT+TGG - chr2.4:73373604-73373623 MS.gene01229:CDS 35.0%
TAAAAATGAGACGAAAAGGG+AGG - chr2.4:73372426-73372445 MS.gene01229:CDS 35.0%
TACAATAGCGAGGAATTTCT+CGG - chr2.4:73373753-73373772 MS.gene01229:CDS 35.0%
TAGCCAAAGTCAACAGATTA+AGG - chr2.4:73372821-73372840 MS.gene01229:CDS 35.0%
TGAATTAATGGGACCATTAG+CGG + chr2.4:73373151-73373170 None:intergenic 35.0%
TGGGCTAAAATCAATACAAC+GGG + chr2.4:73373967-73373986 None:intergenic 35.0%
TTAGAGAAAACAGTGTAGGA+TGG + chr2.4:73373912-73373931 None:intergenic 35.0%
! AAGCTTGAGAGCTTTTTGTT+TGG - chr2.4:73372574-73372593 MS.gene01229:CDS 35.0%
! AGAAAGATGCTTTTCGAATG+GGG + chr2.4:73373293-73373312 None:intergenic 35.0%
! ATACACAAAGGTGTAGATGA+AGG - chr2.4:73373648-73373667 MS.gene01229:CDS 35.0%
! CAAGAAAGATGCTTTTCGAA+TGG + chr2.4:73373295-73373314 None:intergenic 35.0%
!! AAGGCTAGAGTTGATAAGAT+TGG - chr2.4:73373378-73373397 MS.gene01229:CDS 35.0%
!! AGCTTGAGAGCTTTTTGTTT+GGG - chr2.4:73372575-73372594 MS.gene01229:CDS 35.0%
!! CTTCCTTAATCTGTTGACTT+TGG + chr2.4:73372827-73372846 None:intergenic 35.0%
!! GAAGAGTATTGAAGTGTTTG+AGG - chr2.4:73373422-73373441 MS.gene01229:CDS 35.0%
!! GCTCTAAAAATGAGACGAAA+AGG - chr2.4:73372422-73372441 MS.gene01229:CDS 35.0%
!! TGATGCTTTGAAAAGAAAGC+CGG - chr2.4:73372759-73372778 MS.gene01229:CDS 35.0%
AAAAGGGAGGAAGTTGTGAA+AGG - chr2.4:73372439-73372458 MS.gene01229:CDS 40.0%
AAAGAGGAAGCTAGATGGAA+AGG - chr2.4:73372513-73372532 MS.gene01229:CDS 40.0%
AAAGGAAGAACAGTTGGCAA+AGG - chr2.4:73372531-73372550 MS.gene01229:CDS 40.0%
AAATGAGGTTGAAGATGGGA+AGG - chr2.4:73372462-73372481 MS.gene01229:CDS 40.0%
ACAATAGCGAGGAATTTCTC+GGG - chr2.4:73373754-73373773 MS.gene01229:CDS 40.0%
ACGAAAAAGAGGAAGCTAGA+TGG - chr2.4:73372508-73372527 MS.gene01229:CDS 40.0%
AGATGGAAAGGAAGAACAGT+TGG - chr2.4:73372525-73372544 MS.gene01229:CDS 40.0%
AGTAGCTCAAAATTGTTGCC+AGG - chr2.4:73373081-73373100 MS.gene01229:CDS 40.0%
CAGAGTATGTGTCAAGATTG+AGG - chr2.4:73372884-73372903 MS.gene01229:CDS 40.0%
CATCTTCTTGGTAAACAGTG+AGG + chr2.4:73372711-73372730 None:intergenic 40.0%
CCAGTTAGAGAAAACAGTGT+AGG + chr2.4:73373916-73373935 None:intergenic 40.0%
CTCTATTCACCTCCTGAATT+TGG - chr2.4:73372601-73372620 MS.gene01229:CDS 40.0%
CTCTTTGTACTTCTCGAGTA+GGG - chr2.4:73373688-73373707 MS.gene01229:CDS 40.0%
GAATTAATGGGACCATTAGC+GGG + chr2.4:73373150-73373169 None:intergenic 40.0%
GAGGATGAGGATTTGATATC+TGG - chr2.4:73372859-73372878 MS.gene01229:CDS 40.0%
GATTCAGGGTCAAAAATGGA+TGG - chr2.4:73372952-73372971 MS.gene01229:CDS 40.0%
GGAAGTTGTGAAAGGAAATG+AGG - chr2.4:73372447-73372466 MS.gene01229:CDS 40.0%
GGACAAAAATTGTTAAGCGC+TGG - chr2.4:73373579-73373598 MS.gene01229:CDS 40.0%
GGAGTTTGATGTTGATACTG+AGG - chr2.4:73372483-73372502 MS.gene01229:CDS 40.0%
GGGCTAAAATCAATACAACG+GGG + chr2.4:73373966-73373985 None:intergenic 40.0%
TATTATCACCTGAGACCCAT+CGG + chr2.4:73373319-73373338 None:intergenic 40.0%
TCACCTCCTGAATTTGGAAA+GGG - chr2.4:73372607-73372626 MS.gene01229:CDS 40.0%
TCAGTTCAGTTCCATCGAAA+TGG - chr2.4:73373168-73373187 MS.gene01229:CDS 40.0%
TCTCAGAACAAATGAAGACC+TGG - chr2.4:73373050-73373069 MS.gene01229:CDS 40.0%
TTCACCTCCTGAATTTGGAA+AGG - chr2.4:73372606-73372625 MS.gene01229:CDS 40.0%
TTCGAGTATTCAAGATGTCC+TGG + chr2.4:73373102-73373121 None:intergenic 40.0%
TTTGAAAAGAAAGCCGGTGT+GGG - chr2.4:73372765-73372784 MS.gene01229:CDS 40.0%
! ATAGCTTTTGTGGGCAATGA+AGG - chr2.4:73373465-73373484 MS.gene01229:CDS 40.0%
! CCTACACTGTTTTCTCTAAC+TGG - chr2.4:73373913-73373932 MS.gene01229:CDS 40.0%
! GTTGGTACTCTGAAGATGAA+CGG - chr2.4:73373525-73373544 MS.gene01229:CDS 40.0%
! TTCTTACTGCTGGATTAGAC+CGG - chr2.4:73373199-73373218 MS.gene01229:CDS 40.0%
!! CAAAAATTGTTAAGCGCTGG+TGG - chr2.4:73373582-73373601 MS.gene01229:CDS 40.0%
!!! CGTTGTATTGATTTTAGCCC+AGG - chr2.4:73373966-73373985 MS.gene01229:CDS 40.0%
!!! TGTATTGATTTTAGCCCAGG+TGG - chr2.4:73373969-73373988 MS.gene01229:CDS 40.0%
AAGCATCTTTCTTGCCCGAT+GGG - chr2.4:73373301-73373320 MS.gene01229:CDS 45.0%
AAGGAAGTTAAGGAAGGAGG+AGG - chr2.4:73372840-73372859 MS.gene01229:CDS 45.0%
AATAGCGAGGAATTTCTCGG+GGG - chr2.4:73373756-73373775 MS.gene01229:CDS 45.0%
ACCATTGGGATCTGAGAACA+AGG - chr2.4:73373619-73373638 MS.gene01229:CDS 45.0%
ACCTCCTGAATTTGGAAAGG+GGG - chr2.4:73372609-73372628 MS.gene01229:CDS 45.0%
ATGGCTGTAGGAAATGCTTC+AGG - chr2.4:73373999-73374018 MS.gene01229:CDS 45.0%
ATTATCACCTGAGACCCATC+GGG + chr2.4:73373318-73373337 None:intergenic 45.0%
CAATACAACGGGGATAATGC+AGG + chr2.4:73373956-73373975 None:intergenic 45.0%
CAATAGCGAGGAATTTCTCG+GGG - chr2.4:73373755-73373774 MS.gene01229:CDS 45.0%
CACCTCCTGAATTTGGAAAG+GGG - chr2.4:73372608-73372627 MS.gene01229:CDS 45.0%
CTGAGAAGTCAGCATCTTCT+TGG + chr2.4:73372723-73372742 None:intergenic 45.0%
GCTCTTTGTACTTCTCGAGT+AGG - chr2.4:73373687-73373706 MS.gene01229:CDS 45.0%
GCTGGATTCAGGGTCAAAAA+TGG - chr2.4:73372948-73372967 MS.gene01229:CDS 45.0%
GGGTCTCAGGTGATAATATC+AGG - chr2.4:73373321-73373340 MS.gene01229:CDS 45.0%
GGTGGATGAGGAAGAAGAAA+AGG - chr2.4:73372786-73372805 MS.gene01229:CDS 45.0%
TCATCCCCCTTTCCAAATTC+AGG + chr2.4:73372616-73372635 None:intergenic 45.0%
TCCTTGTTCTCAGATCCCAA+TGG + chr2.4:73373623-73373642 None:intergenic 45.0%
TGAGGAGACTATCTGCAGAA+CGG + chr2.4:73372693-73372712 None:intergenic 45.0%
TTCGAATGGGGCAATCTTCA+AGG + chr2.4:73373281-73373300 None:intergenic 45.0%
TTGAGCTACTCTTCACAACC+AGG + chr2.4:73373071-73373090 None:intergenic 45.0%
! AAAGCATCTTTCTTGCCCGA+TGG - chr2.4:73373300-73373319 MS.gene01229:CDS 45.0%
! CTTTGAAAAGAAAGCCGGTG+TGG - chr2.4:73372764-73372783 MS.gene01229:CDS 45.0%
! GATAAGATTGGTCCGCTGTT+GGG - chr2.4:73373390-73373409 MS.gene01229:CDS 45.0%
! TGATAAGATTGGTCCGCTGT+TGG - chr2.4:73373389-73373408 MS.gene01229:CDS 45.0%
!! ATTGATTTTAGCCCAGGTGG+TGG - chr2.4:73373972-73373991 MS.gene01229:CDS 45.0%
AATAAAGCTGCTGTGAGCCG+AGG - chr2.4:73373006-73373025 MS.gene01229:CDS 50.0%
ACAGCCATAAAACCACCACC+TGG + chr2.4:73373987-73374006 None:intergenic 50.0%
CAGCCATAAAACCACCACCT+GGG + chr2.4:73373986-73374005 None:intergenic 50.0%
CATCCAGATCCTCGTAACCT+CGG + chr2.4:73373026-73373045 None:intergenic 50.0%
GAAGCAGCTGACCATTTCGA+TGG + chr2.4:73373182-73373201 None:intergenic 50.0%
GTTAAGGAAGGAGGAGGATG+AGG - chr2.4:73372846-73372865 MS.gene01229:CDS 50.0%
TCCCCCTTTCCAAATTCAGG+AGG + chr2.4:73372613-73372632 None:intergenic 50.0%
TTCTCTTCCCTACCCAACAG+CGG + chr2.4:73373405-73373424 None:intergenic 50.0%
! AAAGGGGGATGATGATGAGG+TGG - chr2.4:73372624-73372643 MS.gene01229:CDS 50.0%
! GTAGGGACACATTTTGCAGC+TGG - chr2.4:73373705-73373724 MS.gene01229:CDS 50.0%
! TGGAAAGGGGGATGATGATG+AGG - chr2.4:73372621-73372640 MS.gene01229:CDS 50.0%
! TTTGCAGCTGGTTCAGCTAG+TGG - chr2.4:73373717-73373736 MS.gene01229:CDS 50.0%
!! TAGCCCAGGTGGTGGTTTTA+TGG - chr2.4:73373980-73373999 MS.gene01229:CDS 50.0%
!! TTGTTAAGCGCTGGTGGTGA+TGG - chr2.4:73373588-73373607 MS.gene01229:CDS 50.0%
!!! GGTGGTGGTTTTATGGCTGT+AGG - chr2.4:73373987-73374006 MS.gene01229:CDS 50.0%
AGAAGCCTTTGCAGAAGCCG+CGG + chr2.4:73372653-73372672 None:intergenic 55.0%
AGCTCAACCGAGGAACTGAC+TGG - chr2.4:73372920-73372939 MS.gene01229:CDS 55.0%
CAGCATGCAAAGCTCAACCG+AGG - chr2.4:73372910-73372929 MS.gene01229:CDS 55.0%
GAAAAGAAAGCCGGTGTGGG+TGG - chr2.4:73372768-73372787 MS.gene01229:CDS 55.0%
GCTCAACCGAGGAACTGACT+GGG - chr2.4:73372921-73372940 MS.gene01229:CDS 55.0%
GGTCAGCTGCTTCTTACTGC+TGG - chr2.4:73373189-73373208 MS.gene01229:CDS 55.0%
TCTTCCTCATCCACCCACAC+CGG + chr2.4:73372781-73372800 None:intergenic 55.0%
TGCAGGCTCGCATTTGAACG+CGG + chr2.4:73373939-73373958 None:intergenic 55.0%
TTTCTTGCCCGATGGGTCTC+AGG - chr2.4:73373308-73373327 MS.gene01229:CDS 55.0%
! AGATTGGTCCGCTGTTGGGT+AGG - chr2.4:73373394-73373413 MS.gene01229:CDS 55.0%
! GATTGGTCCGCTGTTGGGTA+GGG - chr2.4:73373395-73373414 MS.gene01229:CDS 55.0%
! TGATGATGAGGTGGATGCCG+CGG - chr2.4:73372633-73372652 MS.gene01229:CDS 55.0%
AAAGCCGGTGTGGGTGGATG+AGG - chr2.4:73372774-73372793 MS.gene01229:CDS 60.0%
ACTGGGCGCAGCTGGATTCA+GGG - chr2.4:73372938-73372957 MS.gene01229:CDS 60.0%
GAGCCGAGGTTACGAGGATC+TGG - chr2.4:73373020-73373039 MS.gene01229:CDS 60.0%
TGCTGTGAGCCGAGGTTACG+AGG - chr2.4:73373014-73373033 MS.gene01229:CDS 60.0%
AGGAACTGACTGGGCGCAGC+TGG - chr2.4:73372930-73372949 MS.gene01229:CDS 65.0%
GACTGGGCGCAGCTGGATTC+AGG - chr2.4:73372937-73372956 MS.gene01229:CDS 65.0%
GCTGCGCCCAGTCAGTTCCT+CGG + chr2.4:73372930-73372949 None:intergenic 65.0%
GGATGCCGCGGCTTCTGCAA+AGG - chr2.4:73372645-73372664 MS.gene01229:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 73372397 73374064 73372397 ID=MS.gene01229
chr2.4 mRNA 73372397 73374064 73372397 ID=MS.gene01229.t1;Parent=MS.gene01229
chr2.4 exon 73372397 73374064 73372397 ID=MS.gene01229.t1.exon1;Parent=MS.gene01229.t1
chr2.4 CDS 73372397 73374064 73372397 ID=cds.MS.gene01229.t1;Parent=MS.gene01229.t1
Gene Sequence

>MS.gene01229

ATGAGTTTAATATCTCAAAATGCTCGCTCTAAAAATGAGACGAAAAGGGAGGAAGTTGTGAAAGGAAATGAGGTTGAAGATGGGAAGGAGTTTGATGTTGATACTGAGGAAACGAAAAAGAGGAAGCTAGATGGAAAGGAAGAACAGTTGGCAAAGGAACAAGTTAAAGAAATGAAAAAGCTTGAGAGCTTTTTGTTTGGGTCTCTCTATTCACCTCCTGAATTTGGAAAGGGGGATGATGATGAGGTGGATGCCGCGGCTTCTGCAAAGGCTTCTAATTTGTTCTTCACTGACCGTTCTGCAGATAGTCTCCTCACTGTTTACCAAGAAGATGCTGACTTCTCAGATGAAAGTGATAATGATGATGCTTTGAAAAGAAAGCCGGTGTGGGTGGATGAGGAAGAAGAAAAGGTTACTGTAAACATAGCCAAAGTCAACAGATTAAGGAAGTTAAGGAAGGAGGAGGATGAGGATTTGATATCTGGTTCAGAGTATGTGTCAAGATTGAGGGCACAGCATGCAAAGCTCAACCGAGGAACTGACTGGGCGCAGCTGGATTCAGGGTCAAAAATGGATGGATCTTCTGATGATGAATTGACAGATGATGAAAATAAAGCTGCTGTGAGCCGAGGTTACGAGGATCTGGATGATATTCTCAGAACAAATGAAGACCTGGTTGTGAAGAGTAGCTCAAAATTGTTGCCAGGACATCTTGAATACTCGAAGCTTGTCGATGCAAATATACAAGATCCCGCTAATGGTCCCATTAATTCAGTTCAGTTCCATCGAAATGGTCAGCTGCTTCTTACTGCTGGATTAGACCGGAAGCTTAAATTTTTTCAAATTGATGGCAAACGGAACACCAAGATTGAAAGTATCTTCCTTGAAGATTGCCCCATTCGAAAAGCATCTTTCTTGCCCGATGGGTCTCAGGTGATAATATCAGGAAGAAGAAAGTTCTTTTATAGTCTTGATTTAGTTAAGGCTAGAGTTGATAAGATTGGTCCGCTGTTGGGTAGGGAAGAGAAGAGTATTGAAGTGTTTGAGGTCTCTCCTGATTCTCAAATGATAGCTTTTGTGGGCAATGAAGGTTATATTTTACTGGTTTCAACAAAGTCGAAGCAATTAGTTGGTACTCTGAAGATGAACGGAACAGTCCGTTCATTAGCTTTTACTGAAGATGGACAAAAATTGTTAAGCGCTGGTGGTGATGGTCATATTTACCATTGGGATCTGAGAACAAGGACTTGTATACACAAAGGTGTAGATGAAGGCTGTTTAAACGGCACAGCTCTTTGTACTTCTCGAGTAGGGACACATTTTGCAGCTGGTTCAGCTAGTGGAGTTGTGAATATTTACAATAGCGAGGAATTTCTCGGGGGAAAGAGAAAGCCTATCAAGACCATTGATAATCTGAATACTGAAGTGGATTTCATGAAATTCAATCATGACTCTCAAATACTAGCAATCTGTTCAAGGGCGAAGAACAGCAGTTTGAAATTAGTACATATACCATCCTACACTGTTTTCTCTAACTGGCCGCGTTCAAATGCGAGCCTGCATTATCCCCGTTGTATTGATTTTAGCCCAGGTGGTGGTTTTATGGCTGTAGGAAATGCTTCAGGCAAAGTGTTACTGTATAAGTTGCACCATTATCACCATGCCTGA
Protein sequence

>MS.gene01229.t1

MSLISQNARSKNETKREEVVKGNEVEDGKEFDVDTEETKKRKLDGKEEQLAKEQVKEMKKLESFLFGSLYSPPEFGKGDDDEVDAAASAKASNLFFTDRSADSLLTVYQEDADFSDESDNDDALKRKPVWVDEEEEKVTVNIAKVNRLRKLRKEEDEDLISGSEYVSRLRAQHAKLNRGTDWAQLDSGSKMDGSSDDELTDDENKAAVSRGYEDLDDILRTNEDLVVKSSSKLLPGHLEYSKLVDANIQDPANGPINSVQFHRNGQLLLTAGLDRKLKFFQIDGKRNTKIESIFLEDCPIRKASFLPDGSQVIISGRRKFFYSLDLVKARVDKIGPLLGREEKSIEVFEVSPDSQMIAFVGNEGYILLVSTKSKQLVGTLKMNGTVRSLAFTEDGQKLLSAGGDGHIYHWDLRTRTCIHKGVDEGCLNGTALCTSRVGTHFAAGSASGVVNIYNSEEFLGGKRKPIKTIDNLNTEVDFMKFNHDSQILAICSRAKNSSLKLVHIPSYTVFSNWPRSNASLHYPRCIDFSPGGGFMAVGNASGKVLLYKLHHYHHA