AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02213

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02213.t1 MTR_2g009800 98.378 555 9 0 1 555 1 555 0.0 1122
MS.gene02213.t1 MTR_2g101620 91.351 555 48 0 1 555 1 555 0.0 1048
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02213.t1 AT5G14050 61.947 565 186 8 1 555 1 546 0.0 676

Find 115 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTATTCACCTCCTGAATT+TGG 0.168421 2.1:-73347148 MS.gene02213:CDS
ATGGAACTGAACTGAATTAA+TGG 0.221155 2.1:+73346589 None:intergenic
TAGCCAAAGTCAACAGATTA+AGG 0.291410 2.1:-73346928 MS.gene02213:CDS
CTGAGAAGTCAGCATCTTCT+TGG 0.323321 2.1:+73347029 None:intergenic
AAAGCTATCATTTGAGAATC+AGG 0.336697 2.1:+73346299 None:intergenic
AAGGAAGATACTTTCAATCT+TGG 0.340340 2.1:+73346490 None:intergenic
GAAGAGTATTGAAGTGTTTG+AGG 0.342182 2.1:-73346327 MS.gene02213:CDS
AGATTATCAATGGTCTTGAT+AGG 0.358024 2.1:+73345960 None:intergenic
TACTTCAGGCATGGTGATAA+TGG 0.362722 2.1:+73345703 None:intergenic
GGGGATGAGGATTTGATATC+TGG 0.363071 2.1:-73346890 MS.gene02213:CDS
GGTCAGCTGCTTCTTGCTGC+TGG 0.365476 2.1:-73346560 MS.gene02213:CDS
TCAACAGATTAAGGAAGTTA+AGG 0.371537 2.1:-73346919 MS.gene02213:CDS
AGGAACTGACTGGGCGCAGC+TGG 0.391668 2.1:-73346819 MS.gene02213:CDS
TGGGCTAAAATCAATACAAC+GGG 0.399262 2.1:+73345785 None:intergenic
TGGAACTGAACTGAATTAAT+GGG 0.402013 2.1:+73346590 None:intergenic
CTTCCTTAATCTGTTGACTT+TGG 0.407960 2.1:+73346925 None:intergenic
GGGTCTCAGGTGATAATATC+AGG 0.411556 2.1:-73346428 MS.gene02213:CDS
TTTCTTGCCCGATGGGTCTC+AGG 0.414581 2.1:-73346441 MS.gene02213:CDS
ATGGCTGTAGGAAATGCTTC+AGG 0.420095 2.1:-73345750 MS.gene02213:CDS
TTATAGTCTTGATTTAGTTA+AGG 0.420893 2.1:-73346390 MS.gene02213:CDS
TTCGAATGGGGCAATCTTCA+AGG 0.424695 2.1:+73346471 None:intergenic
TCAGTTCAGTTCCATCGAAA+TGG 0.438541 2.1:-73346581 MS.gene02213:CDS
AAATACTAGCAATCTGTTCA+AGG 0.442805 2.1:-73345896 MS.gene02213:CDS
TTCAGTATTCAGATTATCAA+TGG 0.445525 2.1:+73345950 None:intergenic
TACAATAGCGAGGAATTTCT+CGG 0.460074 2.1:-73345996 MS.gene02213:CDS
GAGCCGAGGTTACGAGGATC+TGG 0.461776 2.1:-73346729 MS.gene02213:CDS
TTAAGGAAGTTAAGGAAGGA+GGG 0.465681 2.1:-73346911 MS.gene02213:CDS
GTAGATGAAGGCTGTTTAAA+CGG 0.466326 2.1:-73346089 MS.gene02213:CDS
TCACCTCCTGAATTTGGAAA+GGG 0.467907 2.1:-73347142 MS.gene02213:CDS
GCTGCAAAATGTGTCCCTAC+TGG 0.467974 2.1:+73346047 None:intergenic
AAGGCTAGAGTTGATAAGAT+TGG 0.470242 2.1:-73346371 MS.gene02213:CDS
AGCTCAACCGAGGAACTGAC+TGG 0.472580 2.1:-73346829 MS.gene02213:CDS
TGATGGTCATATTTACCATT+GGG 0.474225 2.1:-73346144 MS.gene02213:CDS
GATAAGATTGGTCCGCTGTT+GGG 0.475592 2.1:-73346359 MS.gene02213:CDS
AAGCATCTTTCTTGCCCGAT+GGG 0.479466 2.1:-73346448 MS.gene02213:CDS
TTTGCAGCTGGTTCAGCTAG+TGG 0.484592 2.1:-73346032 MS.gene02213:CDS
GAATTAATGGGACCGTTAGC+GGG 0.485707 2.1:+73346602 None:intergenic
GTGATGGTCATATTTACCAT+TGG 0.489547 2.1:-73346145 MS.gene02213:CDS
AAAGCCGGTGTGGGTGGATG+AGG 0.489666 2.1:-73346975 MS.gene02213:CDS
CAGAGTATGTGTCAAGATTG+AGG 0.492768 2.1:-73346865 MS.gene02213:CDS
TAAGGAAGTTAAGGAAGGAG+GGG 0.494442 2.1:-73346910 MS.gene02213:CDS
ACAAAGTCGAAGCAATTAGT+TGG 0.498298 2.1:-73346242 MS.gene02213:CDS
ACAATAGCGAGGAATTTCTC+GGG 0.503579 2.1:-73345995 MS.gene02213:CDS
AGAGTATGTGTCAAGATTGA+GGG 0.513752 2.1:-73346864 MS.gene02213:CDS
ATTAAGGAAGTTAAGGAAGG+AGG 0.514380 2.1:-73346912 MS.gene02213:CDS
TGATAAGATTGGTCCGCTGT+TGG 0.514998 2.1:-73346360 MS.gene02213:CDS
GATTGGTCCGCTGTTGGGTA+GGG 0.516250 2.1:-73346354 MS.gene02213:CDS
TGAATTAATGGGACCGTTAG+CGG 0.516834 2.1:+73346601 None:intergenic
GTATGCCGCGGCTTCTGCAA+AGG 0.519704 2.1:-73347104 MS.gene02213:CDS
GCTGCGCCCAGTCAGTTCCT+CGG 0.520974 2.1:+73346822 None:intergenic
GGTGGATGAGGAAGAAGAAA+AGG 0.532095 2.1:-73346963 MS.gene02213:CDS
TTCGAGTATTCAAGATGTCC+TGG 0.533881 2.1:+73346650 None:intergenic
AAAGGAAATGAGGTTGAAGA+TGG 0.535620 2.1:-73347292 MS.gene02213:CDS
ATCTTCTGATGATGAATTGA+CGG 0.535703 2.1:-73346774 MS.gene02213:CDS
AGATTGGTCCGCTGTTGGGT+AGG 0.539038 2.1:-73346355 MS.gene02213:CDS
TTCACCTCCTGAATTTGGAA+AGG 0.542160 2.1:-73347143 MS.gene02213:CDS
GCTCTTTGTACTTCTCCAGT+AGG 0.543723 2.1:-73346062 MS.gene02213:CDS
ATTATCACCTGAGACCCATC+GGG 0.550131 2.1:+73346434 None:intergenic
AAGGAAGTTAAGGAAGGAGG+GGG 0.550575 2.1:-73346909 MS.gene02213:CDS
AGATGGAAAGGAAGAACAGT+TGG 0.550771 2.1:-73347224 MS.gene02213:CDS
TTGAGCTACTCTTCACAACC+AGG 0.551752 2.1:+73346681 None:intergenic
CAATACAACGGGGATAATGC+AGG 0.553209 2.1:+73345796 None:intergenic
AAAGAGGAAGCTAGATGGAA+AGG 0.553316 2.1:-73347236 MS.gene02213:CDS
TGATAATCTGAATACTGAAG+TGG 0.554817 2.1:-73345946 MS.gene02213:CDS
TTGTTAAGCGCTGGTGGTGA+TGG 0.557212 2.1:-73346161 MS.gene02213:CDS
AAAGCATCTTTCTTGCCCGA+TGG 0.557386 2.1:-73346449 MS.gene02213:CDS
TTCTTGCTGCTGGATTAGAC+CGG 0.558175 2.1:-73346550 MS.gene02213:CDS
TATTATCACCTGAGACCCAT+CGG 0.558404 2.1:+73346433 None:intergenic
AAATGAGGTTGAAGATGGGA+AGG 0.558756 2.1:-73347287 MS.gene02213:CDS
AAGGAAATGAGGTTGAAGAT+GGG 0.562237 2.1:-73347291 MS.gene02213:CDS
AGTAGCTCAAAATTGTTGCC+AGG 0.564255 2.1:-73346668 MS.gene02213:CDS
GGAGTTTGATGTTGATACTG+AGG 0.566399 2.1:-73347266 MS.gene02213:CDS
CTCTTTGTACTTCTCCAGTA+GGG 0.567092 2.1:-73346061 MS.gene02213:CDS
CAGATTAAGGAAGTTAAGGA+AGG 0.571312 2.1:-73346915 MS.gene02213:CDS
ACCTCCTGAATTTGGAAAGG+GGG 0.574743 2.1:-73347140 MS.gene02213:CDS
TGCTGTGAGCCGAGGTTACG+AGG 0.576924 2.1:-73346735 MS.gene02213:CDS
CATCCAGATCCTCGTAACCT+CGG 0.587962 2.1:+73346726 None:intergenic
AATACTAGCAATCTGTTCAA+GGG 0.593971 2.1:-73345895 MS.gene02213:CDS
GGAAGTTGTGAAAGGAAATG+AGG 0.594864 2.1:-73347302 MS.gene02213:CDS
TTTCTGATCTACTTCAGGCA+TGG 0.600646 2.1:+73345694 None:intergenic
GAAGCAGCTGACCATTTCGA+TGG 0.604108 2.1:+73346570 None:intergenic
ATACACAAAGGTGTAGATGA+AGG 0.605326 2.1:-73346101 MS.gene02213:CDS
CTTCAGTAAAAGCTAATGAA+CGG 0.605560 2.1:+73346195 None:intergenic
TGTGAATATTTACAATAGCG+AGG 0.608077 2.1:-73346006 MS.gene02213:CDS
AAAAGGGAGGAAGTTGTGAA+AGG 0.616065 2.1:-73347310 MS.gene02213:CDS
AATAAAGCTGCTGTGAGCCG+AGG 0.616455 2.1:-73346743 MS.gene02213:CDS
AGAAGCCTTTGCAGAAGCCG+CGG 0.617253 2.1:+73347099 None:intergenic
TCTCAGAACAAATGAAGACC+TGG 0.621951 2.1:-73346699 MS.gene02213:CDS
ACCATTGGGATCTGAGAACA+AGG 0.623692 2.1:-73346130 MS.gene02213:CDS
CTGGGCTAAAATCAATACAA+CGG 0.628701 2.1:+73345784 None:intergenic
ACAGCCATAAAACCACCACC+TGG 0.630227 2.1:+73345765 None:intergenic
AATATACAAGATCCCGCTAA+CGG 0.631954 2.1:-73346614 MS.gene02213:CDS
AAAGGAAGAACAGTTGGCAA+AGG 0.637301 2.1:-73347218 MS.gene02213:CDS
CACCTCCTGAATTTGGAAAG+GGG 0.637398 2.1:-73347141 MS.gene02213:CDS
GCTCAACCGAGGAACTGACT+GGG 0.639373 2.1:-73346828 MS.gene02213:CDS
CCAGTTAGAGAAAACAGTGT+AGG 0.639938 2.1:+73345836 None:intergenic
GTTGGTACTCTGAAGATGAA+CGG 0.640176 2.1:-73346224 MS.gene02213:CDS
TTAGAGAAAACAGTGTAGGA+TGG 0.640295 2.1:+73345840 None:intergenic
CAATAGCGAGGAATTTCTCG+GGG 0.640898 2.1:-73345994 MS.gene02213:CDS
GAAAAGAAAGCCGGTGTGGG+TGG 0.643363 2.1:-73346981 MS.gene02213:CDS
TGCAGGCTCGCATTTGAACG+CGG 0.645712 2.1:+73345813 None:intergenic
TCTTCCTCATCCACCCACAC+CGG 0.648855 2.1:+73346971 None:intergenic
CTTTGAAAAGAAAGCCGGTG+TGG 0.649038 2.1:-73346985 MS.gene02213:CDS
TTCTCTTCCCTACCCAACAG+CGG 0.649632 2.1:+73346347 None:intergenic
TGATGCTTTGAAAAGAAAGC+CGG 0.651576 2.1:-73346990 MS.gene02213:CDS
CAGCCATAAAACCACCACCT+GGG 0.668450 2.1:+73345766 None:intergenic
CATCTTCTTGGTAAACAGTG+AGG 0.681529 2.1:+73347041 None:intergenic
TGAGGAGACTATCTGCAGAA+CGG 0.693456 2.1:+73347059 None:intergenic
TTTGAAAAGAAAGCCGGTGT+GGG 0.693980 2.1:-73346984 MS.gene02213:CDS
ACAAGGACTTGTATACACAA+AGG 0.694652 2.1:-73346113 MS.gene02213:CDS
TGATGATGAGGTGTATGCCG+CGG 0.712264 2.1:-73347116 MS.gene02213:CDS
CAGCATGCAAAGCTCAACCG+AGG 0.712589 2.1:-73346839 MS.gene02213:CDS
TCCTTGTTCTCAGATCCCAA+TGG 0.719029 2.1:+73346129 None:intergenic
AATAGCGAGGAATTTCTCGG+GGG 0.758971 2.1:-73345993 MS.gene02213:CDS
GGGCTAAAATCAATACAACG+GGG 0.814054 2.1:+73345786 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTAAATTTTTTCAAATTGA+TGG - chr2.1:73346535-73346554 MS.gene02213:CDS 15.0%
!! TTTGAAAAAATTTAAGCTTC+CGG + chr2.1:73346531-73346550 None:intergenic 20.0%
!!! TTATAGTCTTGATTTAGTTA+AGG - chr2.1:73346669-73346688 MS.gene02213:CDS 20.0%
! TTCAGTATTCAGATTATCAA+TGG + chr2.1:73347112-73347131 None:intergenic 25.0%
!! CAATGAAGGTTATATTTTAC+TGG - chr2.1:73346789-73346808 MS.gene02213:CDS 25.0%
!!! TTTTTCAAATTGATGGCAAA+CGG - chr2.1:73346542-73346561 MS.gene02213:CDS 25.0%
AAAGCTATCATTTGAGAATC+AGG + chr2.1:73346763-73346782 None:intergenic 30.0%
AAATACTAGCAATCTGTTCA+AGG - chr2.1:73347163-73347182 MS.gene02213:CDS 30.0%
AAGGAAGATACTTTCAATCT+TGG + chr2.1:73346572-73346591 None:intergenic 30.0%
AATACTAGCAATCTGTTCAA+GGG - chr2.1:73347164-73347183 MS.gene02213:CDS 30.0%
ATGGAACTGAACTGAATTAA+TGG + chr2.1:73346473-73346492 None:intergenic 30.0%
CTTCAGTAAAAGCTAATGAA+CGG + chr2.1:73346867-73346886 None:intergenic 30.0%
TCAACAGATTAAGGAAGTTA+AGG - chr2.1:73346140-73346159 MS.gene02213:CDS 30.0%
TGATAATCTGAATACTGAAG+TGG - chr2.1:73347113-73347132 MS.gene02213:CDS 30.0%
TGATGGTCATATTTACCATT+GGG - chr2.1:73346915-73346934 MS.gene02213:CDS 30.0%
TGGAACTGAACTGAATTAAT+GGG + chr2.1:73346472-73346491 None:intergenic 30.0%
TGTGAATATTTACAATAGCG+AGG - chr2.1:73347053-73347072 MS.gene02213:CDS 30.0%
! AAGAAAGATGCTTTTCGAAT+GGG + chr2.1:73346604-73346623 None:intergenic 30.0%
! AGATTATCAATGGTCTTGAT+AGG + chr2.1:73347102-73347121 None:intergenic 30.0%
! ATCTTCTGATGATGAATTGA+CGG - chr2.1:73346285-73346304 MS.gene02213:CDS 30.0%
! CTCTAAAAATGAGACGAAAA+GGG - chr2.1:73345733-73345752 MS.gene02213:CDS 30.0%
! TCTCAAATGATAGCTTTTGT+GGG - chr2.1:73346766-73346785 MS.gene02213:CDS 30.0%
! TTCTCAAATGATAGCTTTTG+TGG - chr2.1:73346765-73346784 MS.gene02213:CDS 30.0%
!!! TCATTAGCTTTTACTGAAGA+TGG - chr2.1:73346868-73346887 MS.gene02213:CDS 30.0%
AAAGGAAATGAGGTTGAAGA+TGG - chr2.1:73345767-73345786 MS.gene02213:CDS 35.0%
AAGGAAATGAGGTTGAAGAT+GGG - chr2.1:73345768-73345787 MS.gene02213:CDS 35.0%
AATATACAAGATCCCGCTAA+CGG - chr2.1:73346445-73346464 MS.gene02213:CDS 35.0%
ACAAAGTCGAAGCAATTAGT+TGG - chr2.1:73346817-73346836 MS.gene02213:CDS 35.0%
ACAAGGACTTGTATACACAA+AGG - chr2.1:73346946-73346965 MS.gene02213:CDS 35.0%
AGAGTATGTGTCAAGATTGA+GGG - chr2.1:73346195-73346214 MS.gene02213:CDS 35.0%
ATACTGAGGAAACGAAAAAG+AGG - chr2.1:73345807-73345826 MS.gene02213:CDS 35.0%
ATTAAGGAAGTTAAGGAAGG+AGG - chr2.1:73346147-73346166 MS.gene02213:CDS 35.0%
CAGATTAAGGAAGTTAAGGA+AGG - chr2.1:73346144-73346163 MS.gene02213:CDS 35.0%
CTGGGCTAAAATCAATACAA+CGG + chr2.1:73347278-73347297 None:intergenic 35.0%
GATTCAGAGTCAAAAATGGA+TGG - chr2.1:73346262-73346281 MS.gene02213:CDS 35.0%
GTAGATGAAGGCTGTTTAAA+CGG - chr2.1:73346970-73346989 MS.gene02213:CDS 35.0%
GTGATGGTCATATTTACCAT+TGG - chr2.1:73346914-73346933 MS.gene02213:CDS 35.0%
TAAAAATGAGACGAAAAGGG+AGG - chr2.1:73345736-73345755 MS.gene02213:CDS 35.0%
TACAATAGCGAGGAATTTCT+CGG - chr2.1:73347063-73347082 MS.gene02213:CDS 35.0%
TAGCCAAAGTCAACAGATTA+AGG - chr2.1:73346131-73346150 MS.gene02213:CDS 35.0%
TGGGCTAAAATCAATACAAC+GGG + chr2.1:73347277-73347296 None:intergenic 35.0%
TTAAGGAAGTTAAGGAAGGA+GGG - chr2.1:73346148-73346167 MS.gene02213:CDS 35.0%
TTAGAGAAAACAGTGTAGGA+TGG + chr2.1:73347222-73347241 None:intergenic 35.0%
! AAGCTTGAGAGCTTTTTGTT+TGG - chr2.1:73345884-73345903 MS.gene02213:CDS 35.0%
! AGAAAGATGCTTTTCGAATG+GGG + chr2.1:73346603-73346622 None:intergenic 35.0%
! ATACACAAAGGTGTAGATGA+AGG - chr2.1:73346958-73346977 MS.gene02213:CDS 35.0%
! CAAGAAAGATGCTTTTCGAA+TGG + chr2.1:73346605-73346624 None:intergenic 35.0%
!! AAGGCTAGAGTTGATAAGAT+TGG - chr2.1:73346688-73346707 MS.gene02213:CDS 35.0%
!! AGCTTGAGAGCTTTTTGTTT+GGG - chr2.1:73345885-73345904 MS.gene02213:CDS 35.0%
!! CTTCCTTAATCTGTTGACTT+TGG + chr2.1:73346137-73346156 None:intergenic 35.0%
!! GAAGAGTATTGAAGTGTTTG+AGG - chr2.1:73346732-73346751 MS.gene02213:CDS 35.0%
!! GCTCTAAAAATGAGACGAAA+AGG - chr2.1:73345732-73345751 MS.gene02213:CDS 35.0%
!! TGATGCTTTGAAAAGAAAGC+CGG - chr2.1:73346069-73346088 MS.gene02213:CDS 35.0%
AAAAGGGAGGAAGTTGTGAA+AGG - chr2.1:73345749-73345768 MS.gene02213:CDS 40.0%
AAAGAGGAAGCTAGATGGAA+AGG - chr2.1:73345823-73345842 MS.gene02213:CDS 40.0%
AAAGGAAGAACAGTTGGCAA+AGG - chr2.1:73345841-73345860 MS.gene02213:CDS 40.0%
AAATGAGGTTGAAGATGGGA+AGG - chr2.1:73345772-73345791 MS.gene02213:CDS 40.0%
ACAATAGCGAGGAATTTCTC+GGG - chr2.1:73347064-73347083 MS.gene02213:CDS 40.0%
ACGAAAAAGAGGAAGCTAGA+TGG - chr2.1:73345818-73345837 MS.gene02213:CDS 40.0%
AGATGGAAAGGAAGAACAGT+TGG - chr2.1:73345835-73345854 MS.gene02213:CDS 40.0%
AGTAGCTCAAAATTGTTGCC+AGG - chr2.1:73346391-73346410 MS.gene02213:CDS 40.0%
CAGAGTATGTGTCAAGATTG+AGG - chr2.1:73346194-73346213 MS.gene02213:CDS 40.0%
CATCTTCTTGGTAAACAGTG+AGG + chr2.1:73346021-73346040 None:intergenic 40.0%
CCAGTTAGAGAAAACAGTGT+AGG + chr2.1:73347226-73347245 None:intergenic 40.0%
CTCTATTCACCTCCTGAATT+TGG - chr2.1:73345911-73345930 MS.gene02213:CDS 40.0%
CTCTTTGTACTTCTCCAGTA+GGG - chr2.1:73346998-73347017 MS.gene02213:CDS 40.0%
GCTGGATTCAGAGTCAAAAA+TGG - chr2.1:73346258-73346277 MS.gene02213:CDS 40.0%
GGAAGTTGTGAAAGGAAATG+AGG - chr2.1:73345757-73345776 MS.gene02213:CDS 40.0%
GGACAAAAATTGTTAAGCGC+TGG - chr2.1:73346889-73346908 MS.gene02213:CDS 40.0%
GGAGTTTGATGTTGATACTG+AGG - chr2.1:73345793-73345812 MS.gene02213:CDS 40.0%
GGGCTAAAATCAATACAACG+GGG + chr2.1:73347276-73347295 None:intergenic 40.0%
TAAGGAAGTTAAGGAAGGAG+GGG - chr2.1:73346149-73346168 MS.gene02213:CDS 40.0%
TATTATCACCTGAGACCCAT+CGG + chr2.1:73346629-73346648 None:intergenic 40.0%
TCACCTCCTGAATTTGGAAA+GGG - chr2.1:73345917-73345936 MS.gene02213:CDS 40.0%
TCAGTTCAGTTCCATCGAAA+TGG - chr2.1:73346478-73346497 MS.gene02213:CDS 40.0%
TCTCAGAACAAATGAAGACC+TGG - chr2.1:73346360-73346379 MS.gene02213:CDS 40.0%
TGAATTAATGGGACCGTTAG+CGG + chr2.1:73346461-73346480 None:intergenic 40.0%
TTCACCTCCTGAATTTGGAA+AGG - chr2.1:73345916-73345935 MS.gene02213:CDS 40.0%
TTCGAGTATTCAAGATGTCC+TGG + chr2.1:73346412-73346431 None:intergenic 40.0%
TTTGAAAAGAAAGCCGGTGT+GGG - chr2.1:73346075-73346094 MS.gene02213:CDS 40.0%
! ATAGCTTTTGTGGGCAATGA+AGG - chr2.1:73346775-73346794 MS.gene02213:CDS 40.0%
! CCTACACTGTTTTCTCTAAC+TGG - chr2.1:73347223-73347242 MS.gene02213:CDS 40.0%
! GTTGGTACTCTGAAGATGAA+CGG - chr2.1:73346835-73346854 MS.gene02213:CDS 40.0%
!! CAAAAATTGTTAAGCGCTGG+TGG - chr2.1:73346892-73346911 MS.gene02213:CDS 40.0%
!!! CGTTGTATTGATTTTAGCCC+AGG - chr2.1:73347276-73347295 MS.gene02213:CDS 40.0%
!!! TGTATTGATTTTAGCCCAGG+TGG - chr2.1:73347279-73347298 MS.gene02213:CDS 40.0%
AAGCATCTTTCTTGCCCGAT+GGG - chr2.1:73346611-73346630 MS.gene02213:CDS 45.0%
AAGGAAGTTAAGGAAGGAGG+GGG - chr2.1:73346150-73346169 MS.gene02213:CDS 45.0%
AATAGCGAGGAATTTCTCGG+GGG - chr2.1:73347066-73347085 MS.gene02213:CDS 45.0%
ACCATTGGGATCTGAGAACA+AGG - chr2.1:73346929-73346948 MS.gene02213:CDS 45.0%
ACCTCCTGAATTTGGAAAGG+GGG - chr2.1:73345919-73345938 MS.gene02213:CDS 45.0%
ATGGCTGTAGGAAATGCTTC+AGG - chr2.1:73347309-73347328 MS.gene02213:CDS 45.0%
ATTATCACCTGAGACCCATC+GGG + chr2.1:73346628-73346647 None:intergenic 45.0%
CAATACAACGGGGATAATGC+AGG + chr2.1:73347266-73347285 None:intergenic 45.0%
CAATAGCGAGGAATTTCTCG+GGG - chr2.1:73347065-73347084 MS.gene02213:CDS 45.0%
CACCTCCTGAATTTGGAAAG+GGG - chr2.1:73345918-73345937 MS.gene02213:CDS 45.0%
CTGAGAAGTCAGCATCTTCT+TGG + chr2.1:73346033-73346052 None:intergenic 45.0%
GAATTAATGGGACCGTTAGC+GGG + chr2.1:73346460-73346479 None:intergenic 45.0%
GCTCTTTGTACTTCTCCAGT+AGG - chr2.1:73346997-73347016 MS.gene02213:CDS 45.0%
GGGGATGAGGATTTGATATC+TGG - chr2.1:73346169-73346188 MS.gene02213:CDS 45.0%
GGGTCTCAGGTGATAATATC+AGG - chr2.1:73346631-73346650 MS.gene02213:CDS 45.0%
GGTGGATGAGGAAGAAGAAA+AGG - chr2.1:73346096-73346115 MS.gene02213:CDS 45.0%
TCATCCCCCTTTCCAAATTC+AGG + chr2.1:73345926-73345945 None:intergenic 45.0%
TCCTTGTTCTCAGATCCCAA+TGG + chr2.1:73346933-73346952 None:intergenic 45.0%
TGAGGAGACTATCTGCAGAA+CGG + chr2.1:73346003-73346022 None:intergenic 45.0%
TTCGAATGGGGCAATCTTCA+AGG + chr2.1:73346591-73346610 None:intergenic 45.0%
TTGAGCTACTCTTCACAACC+AGG + chr2.1:73346381-73346400 None:intergenic 45.0%
! AAAGCATCTTTCTTGCCCGA+TGG - chr2.1:73346610-73346629 MS.gene02213:CDS 45.0%
! CTTTGAAAAGAAAGCCGGTG+TGG - chr2.1:73346074-73346093 MS.gene02213:CDS 45.0%
! GATAAGATTGGTCCGCTGTT+GGG - chr2.1:73346700-73346719 MS.gene02213:CDS 45.0%
! TGATAAGATTGGTCCGCTGT+TGG - chr2.1:73346699-73346718 MS.gene02213:CDS 45.0%
! TTCTTGCTGCTGGATTAGAC+CGG - chr2.1:73346509-73346528 MS.gene02213:CDS 45.0%
!! ATTGATTTTAGCCCAGGTGG+TGG - chr2.1:73347282-73347301 MS.gene02213:CDS 45.0%
AATAAAGCTGCTGTGAGCCG+AGG - chr2.1:73346316-73346335 MS.gene02213:CDS 50.0%
ACAGCCATAAAACCACCACC+TGG + chr2.1:73347297-73347316 None:intergenic 50.0%
CAGCCATAAAACCACCACCT+GGG + chr2.1:73347296-73347315 None:intergenic 50.0%
CATCCAGATCCTCGTAACCT+CGG + chr2.1:73346336-73346355 None:intergenic 50.0%
GAAGCAGCTGACCATTTCGA+TGG + chr2.1:73346492-73346511 None:intergenic 50.0%
GCTGCAAAATGTGTCCCTAC+TGG + chr2.1:73347015-73347034 None:intergenic 50.0%
TCCCCCTTTCCAAATTCAGG+AGG + chr2.1:73345923-73345942 None:intergenic 50.0%
TTCTCTTCCCTACCCAACAG+CGG + chr2.1:73346715-73346734 None:intergenic 50.0%
! GTAGGGACACATTTTGCAGC+TGG - chr2.1:73347015-73347034 MS.gene02213:CDS 50.0%
! TGATGATGAGGTGTATGCCG+CGG - chr2.1:73345943-73345962 MS.gene02213:CDS 50.0%
! TGGAAAGGGGGATGATGATG+AGG - chr2.1:73345931-73345950 MS.gene02213:CDS 50.0%
! TTTGCAGCTGGTTCAGCTAG+TGG - chr2.1:73347027-73347046 MS.gene02213:CDS 50.0%
!! TAGCCCAGGTGGTGGTTTTA+TGG - chr2.1:73347290-73347309 MS.gene02213:CDS 50.0%
!! TTGTTAAGCGCTGGTGGTGA+TGG - chr2.1:73346898-73346917 MS.gene02213:CDS 50.0%
!!! GGTGGTGGTTTTATGGCTGT+AGG - chr2.1:73347297-73347316 MS.gene02213:CDS 50.0%
AGAAGCCTTTGCAGAAGCCG+CGG + chr2.1:73345963-73345982 None:intergenic 55.0%
AGCTCAACCGAGGAACTGAC+TGG - chr2.1:73346230-73346249 MS.gene02213:CDS 55.0%
CAGCATGCAAAGCTCAACCG+AGG - chr2.1:73346220-73346239 MS.gene02213:CDS 55.0%
GAAAAGAAAGCCGGTGTGGG+TGG - chr2.1:73346078-73346097 MS.gene02213:CDS 55.0%
GCTCAACCGAGGAACTGACT+GGG - chr2.1:73346231-73346250 MS.gene02213:CDS 55.0%
GTTAAGGAAGGAGGGGGATG+AGG - chr2.1:73346156-73346175 MS.gene02213:CDS 55.0%
TCTTCCTCATCCACCCACAC+CGG + chr2.1:73346091-73346110 None:intergenic 55.0%
TGCAGGCTCGCATTTGAACG+CGG + chr2.1:73347249-73347268 None:intergenic 55.0%
TTTCTTGCCCGATGGGTCTC+AGG - chr2.1:73346618-73346637 MS.gene02213:CDS 55.0%
! AGATTGGTCCGCTGTTGGGT+AGG - chr2.1:73346704-73346723 MS.gene02213:CDS 55.0%
! GATTGGTCCGCTGTTGGGTA+GGG - chr2.1:73346705-73346724 MS.gene02213:CDS 55.0%
AAAGCCGGTGTGGGTGGATG+AGG - chr2.1:73346084-73346103 MS.gene02213:CDS 60.0%
GAGCCGAGGTTACGAGGATC+TGG - chr2.1:73346330-73346349 MS.gene02213:CDS 60.0%
GGTCAGCTGCTTCTTGCTGC+TGG - chr2.1:73346499-73346518 MS.gene02213:CDS 60.0%
GTATGCCGCGGCTTCTGCAA+AGG - chr2.1:73345955-73345974 MS.gene02213:CDS 60.0%
TGCTGTGAGCCGAGGTTACG+AGG - chr2.1:73346324-73346343 MS.gene02213:CDS 60.0%
AGGAACTGACTGGGCGCAGC+TGG - chr2.1:73346240-73346259 MS.gene02213:CDS 65.0%
GCTGCGCCCAGTCAGTTCCT+CGG + chr2.1:73346240-73346259 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 73345707 73347374 73345707 ID=MS.gene02213
chr2.1 mRNA 73345707 73347374 73345707 ID=MS.gene02213.t1;Parent=MS.gene02213
chr2.1 exon 73345707 73347374 73345707 ID=MS.gene02213.t1.exon1;Parent=MS.gene02213.t1
chr2.1 CDS 73345707 73347374 73345707 ID=cds.MS.gene02213.t1;Parent=MS.gene02213.t1
Gene Sequence

>MS.gene02213

ATGAGTTTAATATCTCAAAATGCTCGCTCTAAAAATGAGACGAAAAGGGAGGAAGTTGTGAAAGGAAATGAGGTTGAAGATGGGAAGGAGTTTGATGTTGATACTGAGGAAACGAAAAAGAGGAAGCTAGATGGAAAGGAAGAACAGTTGGCAAAGGAACAAGTTAAAGAAATGAAAAAGCTTGAGAGCTTTTTGTTTGGGTCTCTCTATTCACCTCCTGAATTTGGAAAGGGGGATGATGATGAGGTGTATGCCGCGGCTTCTGCAAAGGCTTCTAATTTGTTCTTCACTGACCGTTCTGCAGATAGTCTCCTCACTGTTTACCAAGAAGATGCTGACTTCTCAGATGAAAGTGATAATGATGATGCTTTGAAAAGAAAGCCGGTGTGGGTGGATGAGGAAGAAGAAAAGGTTACTGTAAACATAGCCAAAGTCAACAGATTAAGGAAGTTAAGGAAGGAGGGGGATGAGGATTTGATATCTGGTTCAGAGTATGTGTCAAGATTGAGGGCACAGCATGCAAAGCTCAACCGAGGAACTGACTGGGCGCAGCTGGATTCAGAGTCAAAAATGGATGGATCTTCTGATGATGAATTGACGGATGATGAAAATAAAGCTGCTGTGAGCCGAGGTTACGAGGATCTGGATGATATTCTCAGAACAAATGAAGACCTGGTTGTGAAGAGTAGCTCAAAATTGTTGCCAGGACATCTTGAATACTCGAAGCTTGTCGATGCAAATATACAAGATCCCGCTAACGGTCCCATTAATTCAGTTCAGTTCCATCGAAATGGTCAGCTGCTTCTTGCTGCTGGATTAGACCGGAAGCTTAAATTTTTTCAAATTGATGGCAAACGGAACACCAAGATTGAAAGTATCTTCCTTGAAGATTGCCCCATTCGAAAAGCATCTTTCTTGCCCGATGGGTCTCAGGTGATAATATCAGGAAGAAGAAAGTTCTTTTATAGTCTTGATTTAGTTAAGGCTAGAGTTGATAAGATTGGTCCGCTGTTGGGTAGGGAAGAGAAGAGTATTGAAGTGTTTGAGGTCTCTCCTGATTCTCAAATGATAGCTTTTGTGGGCAATGAAGGTTATATTTTACTGGTTTCAACAAAGTCGAAGCAATTAGTTGGTACTCTGAAGATGAACGGAACAGTCCGTTCATTAGCTTTTACTGAAGATGGACAAAAATTGTTAAGCGCTGGTGGTGATGGTCATATTTACCATTGGGATCTGAGAACAAGGACTTGTATACACAAAGGTGTAGATGAAGGCTGTTTAAACGGCACAGCTCTTTGTACTTCTCCAGTAGGGACACATTTTGCAGCTGGTTCAGCTAGTGGAGTTGTGAATATTTACAATAGCGAGGAATTTCTCGGGGGAAAGAGAAAGCCTATCAAGACCATTGATAATCTGAATACTGAAGTGGATTTCATGAAATTCAATCATGACTCTCAAATACTAGCAATCTGTTCAAGGGCGAAGAACAGCAGTTTGAAGTTAGTACATATACCATCCTACACTGTTTTCTCTAACTGGCCGCGTTCAAATGCGAGCCTGCATTATCCCCGTTGTATTGATTTTAGCCCAGGTGGTGGTTTTATGGCTGTAGGAAATGCTTCAGGCAAAGTGTTACTGTATAAGTTGCACCATTATCACCATGCCTGA
Protein sequence

>MS.gene02213.t1

MSLISQNARSKNETKREEVVKGNEVEDGKEFDVDTEETKKRKLDGKEEQLAKEQVKEMKKLESFLFGSLYSPPEFGKGDDDEVYAAASAKASNLFFTDRSADSLLTVYQEDADFSDESDNDDALKRKPVWVDEEEEKVTVNIAKVNRLRKLRKEGDEDLISGSEYVSRLRAQHAKLNRGTDWAQLDSESKMDGSSDDELTDDENKAAVSRGYEDLDDILRTNEDLVVKSSSKLLPGHLEYSKLVDANIQDPANGPINSVQFHRNGQLLLAAGLDRKLKFFQIDGKRNTKIESIFLEDCPIRKASFLPDGSQVIISGRRKFFYSLDLVKARVDKIGPLLGREEKSIEVFEVSPDSQMIAFVGNEGYILLVSTKSKQLVGTLKMNGTVRSLAFTEDGQKLLSAGGDGHIYHWDLRTRTCIHKGVDEGCLNGTALCTSPVGTHFAAGSASGVVNIYNSEEFLGGKRKPIKTIDNLNTEVDFMKFNHDSQILAICSRAKNSSLKLVHIPSYTVFSNWPRSNASLHYPRCIDFSPGGGFMAVGNASGKVLLYKLHHYHHA