AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022304

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022304.t1 MTR_3g077720 97.561 164 4 0 1 164 1 164 3.71e-120 336
MS.gene022304.t1 MTR_3g077730 87.023 131 15 1 32 160 5 135 2.88e-77 227
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022304.t1 AT4G22220 80.392 153 28 2 5 156 7 158 9.46e-85 246
MS.gene022304.t1 AT4G04080 70.253 158 45 2 1 157 1 157 2.16e-79 233
MS.gene022304.t1 AT3G01020 72.662 139 38 0 26 164 25 163 3.86e-71 212

Find 46 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTACTTACGTGTTCTTAA+TGG 0.261409 3.4:-74596419 None:intergenic
GCAGCTACTGAATGGGTAAA+AGG 0.280013 3.4:+74596373 MS.gene022304:intron
GATGCTCGCTTTAAGACTTT+TGG 0.312604 3.4:+74596155 MS.gene022304:CDS
ATGCTGCAGTGAAGCTTAAC+TGG 0.354352 3.4:-74598268 None:intergenic
AGACGCCAAATCCAGCGATT+AGG 0.369036 3.4:+74595961 MS.gene022304:CDS
AGAATCCTAATCGCTGGATT+TGG 0.371565 3.4:-74595966 None:intergenic
TATCGAAGGATCCAACATTC+CGG 0.389446 3.4:-74596034 None:intergenic
CAAATCAAGGTTGATGAGAA+TGG 0.396788 3.4:+74596122 MS.gene022304:CDS
ATCGAAGGATCCAACATTCC+GGG 0.431036 3.4:-74596033 None:intergenic
TGCCTCGGCTACTGCGGCCA+CGG 0.446467 3.4:+74598342 MS.gene022304:CDS
ATGGTGGGAACTGGTCTTGT+TGG 0.461756 3.4:+74596068 MS.gene022304:CDS
AAATCAAGGTTGATGAGAAT+GGG 0.463058 3.4:+74596123 MS.gene022304:CDS
CGCAAAGAAACTCGTGCAAA+CGG 0.463462 3.4:+74595929 MS.gene022304:CDS
AATGATCCGATGGTGGGAAC+TGG 0.473170 3.4:+74596059 MS.gene022304:CDS
TACGACAATCCCCGGAATGT+TGG 0.510499 3.4:+74596023 MS.gene022304:CDS
GCCTCGGCTACTGCGGCCAC+GGG 0.517239 3.4:+74598343 MS.gene022304:CDS
TGTCATGAAGCTTCAAATCA+AGG 0.528073 3.4:+74596109 MS.gene022304:CDS
GCTATTGCTTCATCGTCCGT+TGG 0.529119 3.4:+74596188 MS.gene022304:CDS
CGTGGAAGAATCCTAATCGC+TGG 0.531980 3.4:-74595972 None:intergenic
ATGGGTAAAAGGAAAGCAAA+TGG 0.536082 3.4:+74596384 MS.gene022304:CDS
TGCAGTGAAGCTTAACTGGC+GGG 0.542392 3.4:-74598264 None:intergenic
TCCCGTGGCCGCAGTAGCCG+AGG 0.550070 3.4:-74598344 None:intergenic
CGATAAGAATGATCCGATGG+TGG 0.569162 3.4:+74596052 MS.gene022304:CDS
TTATGAAGCTAAGCGTGCCT+CGG 0.575006 3.4:+74598327 MS.gene022304:CDS
TGGATCACTACGACAATCCC+CGG 0.588923 3.4:+74596015 MS.gene022304:CDS
CTTCATGACATCACCACAAG+CGG 0.590457 3.4:-74596096 None:intergenic
ATTCTACCACGAGAGGGTGG+TGG 0.593190 3.4:+74595995 MS.gene022304:CDS
TAAGCGTGCCTCGGCTACTG+CGG 0.598393 3.4:+74598336 MS.gene022304:CDS
TTCATGACATCACCACAAGC+GGG 0.602959 3.4:-74596095 None:intergenic
AAAAGGAAAGCAAATGGAGG+AGG 0.604892 3.4:+74596390 MS.gene022304:CDS
CATCGGATCATTCTTATCGA+AGG 0.605249 3.4:-74596048 None:intergenic
ACAAGACCAGTTCCCACCAT+CGG 0.608536 3.4:-74596065 None:intergenic
TCACTGCAGCATGCTTGCTG+AGG 0.608636 3.4:+74598279 MS.gene022304:CDS
GATAAGAATGATCCGATGGT+GGG 0.625202 3.4:+74596053 MS.gene022304:CDS
TCCACGATTCTACCACGAGA+GGG 0.625238 3.4:+74595989 MS.gene022304:CDS
TCGAAGGATCCAACATTCCG+GGG 0.626085 3.4:-74596032 None:intergenic
AGTGGCAGATTTGTCTCCCG+TGG 0.628353 3.4:-74598359 None:intergenic
CTGCAGTGAAGCTTAACTGG+CGG 0.631259 3.4:-74598265 None:intergenic
ACCCTCTCGTGGTAGAATCG+TGG 0.631852 3.4:-74595990 None:intergenic
CTTCGATAAGAATGATCCGA+TGG 0.633992 3.4:+74596049 MS.gene022304:CDS
CTTGTTGGTGCACCCGCTTG+TGG 0.637545 3.4:+74596083 MS.gene022304:CDS
AGTGATCCACCACCCTCTCG+TGG 0.652272 3.4:-74596001 None:intergenic
TTCCACGATTCTACCACGAG+AGG 0.658430 3.4:+74595988 MS.gene022304:CDS
CAATGGAAGAAATCATGCAG+TGG 0.672265 3.4:-74598377 None:intergenic
GGTAAAAGGAAAGCAAATGG+AGG 0.675966 3.4:+74596387 MS.gene022304:CDS
ACGATTCTACCACGAGAGGG+TGG 0.684824 3.4:+74595992 MS.gene022304:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATCAAATTCAATAAAA+AGG - chr3.4:74596292-74596311 None:intergenic 10.0%
!! ATAATCAAATTCAATAAAAA+GGG - chr3.4:74596291-74596310 None:intergenic 10.0%
!!! ACAATTTTTCATATAAAAAT+TGG - chr3.4:74596834-74596853 None:intergenic 10.0%
!! CCAAAATATAATAAAACTTA+GGG - chr3.4:74597416-74597435 None:intergenic 15.0%
!!! CCTAAGTTTTATTATATTTT+GGG + chr3.4:74597414-74597433 MS.gene022304:intron 15.0%
!!! GATAATAGAGATTTAAAATA+AGG + chr3.4:74597142-74597161 MS.gene022304:intron 15.0%
!!! TCAGGATTTTTTTTTTTTTA+GGG + chr3.4:74596248-74596267 MS.gene022304:intron 15.0%
!!! TGCTATTTAAAAAAATAGAA+GGG - chr3.4:74596701-74596720 None:intergenic 15.0%
!!! TTCAGGATTTTTTTTTTTTT+AGG + chr3.4:74596247-74596266 MS.gene022304:intron 15.0%
!! AAATTAATAGCTTTCAACAA+TGG + chr3.4:74597171-74597190 MS.gene022304:intron 20.0%
!! ATATAAAAATTGGAATCCAA+AGG - chr3.4:74596824-74596843 None:intergenic 20.0%
!! ATTGTCTATTATAAAACTAG+AGG + chr3.4:74597341-74597360 MS.gene022304:intron 20.0%
!! CCCAAAATATAATAAAACTT+AGG - chr3.4:74597417-74597436 None:intergenic 20.0%
!! GCTATTTAAAAAAATAGAAG+GGG - chr3.4:74596700-74596719 None:intergenic 20.0%
!! TATTTCCAAATACATGATTT+GGG + chr3.4:74596886-74596905 MS.gene022304:intron 20.0%
!! TTGTCTATTATAAAACTAGA+GGG + chr3.4:74597342-74597361 MS.gene022304:intron 20.0%
!!! AAGATATTTTGTTGGTTTTA+TGG + chr3.4:74597612-74597631 MS.gene022304:intron 20.0%
!!! AGTTTTAACAGATCAATTAA+CGG + chr3.4:74597264-74597283 MS.gene022304:intron 20.0%
!!! ATCTATTTTAAGAGACTTTA+GGG + chr3.4:74597311-74597330 MS.gene022304:intron 20.0%
!!! CCCTAAGTTTTATTATATTT+TGG + chr3.4:74597413-74597432 MS.gene022304:intron 20.0%
!!! GTGCTATTTAAAAAAATAGA+AGG - chr3.4:74596702-74596721 None:intergenic 20.0%
!!! GTTTCAGTAAATTAATTTGA+CGG - chr3.4:74596757-74596776 None:intergenic 20.0%
!!! TATCTATTTTAAGAGACTTT+AGG + chr3.4:74597310-74597329 MS.gene022304:intron 20.0%
!!! TTCTGAGTTAATAAGTTTTA+GGG + chr3.4:74596628-74596647 MS.gene022304:intron 20.0%
!!! TTGTTAACAATTTCCTTTTT+TGG - chr3.4:74597478-74597497 None:intergenic 20.0%
! AAAGAAAAAAAGAAACCAGA+AGG - chr3.4:74598047-74598066 None:intergenic 25.0%
! AATAAACTCTTTATCCAAAC+AGG - chr3.4:74597659-74597678 None:intergenic 25.0%
! AATAAGTGCTTATATGATCA+TGG - chr3.4:74597701-74597720 None:intergenic 25.0%
! ACATTTCTTCTTGAAATTGA+TGG + chr3.4:74597534-74597553 MS.gene022304:intron 25.0%
! ATAAACTCTTTATCCAAACA+GGG - chr3.4:74597658-74597677 None:intergenic 25.0%
! ATGTATTTGGAAATACGAAA+AGG - chr3.4:74596881-74596900 None:intergenic 25.0%
! ATTAACGGTATTAACTTAGA+AGG + chr3.4:74597279-74597298 MS.gene022304:intron 25.0%
! ATTATTGTGAATCAGTTCAT+TGG + chr3.4:74596792-74596811 MS.gene022304:intron 25.0%
! CTCTATTATCATAATTCTCT+CGG - chr3.4:74597132-74597151 None:intergenic 25.0%
! GATAAATGCTCAATAAAAAG+AGG + chr3.4:74597010-74597029 MS.gene022304:intron 25.0%
! GTATTTCCAAATACATGATT+TGG + chr3.4:74596885-74596904 MS.gene022304:intron 25.0%
! TCAAATAAGTTAATCCAAAC+AGG + chr3.4:74597909-74597928 MS.gene022304:intron 25.0%
! TCAACTTGTCATCATTAAAA+AGG - chr3.4:74597976-74597995 None:intergenic 25.0%
! TGTCTATTATAAAACTAGAG+GGG + chr3.4:74597343-74597362 MS.gene022304:intron 25.0%
! TTAACGGTATTAACTTAGAA+GGG + chr3.4:74597280-74597299 MS.gene022304:intron 25.0%
! TTATTGTGAATCAGTTCATT+GGG + chr3.4:74596793-74596812 MS.gene022304:intron 25.0%
! TTTGTTAATGCTTCTATGAT+TGG + chr3.4:74598165-74598184 MS.gene022304:intron 25.0%
!! TATATGAATTTTGTAGAAGG+TGG + chr3.4:74597557-74597576 MS.gene022304:intron 25.0%
!! TGGTATATGAATTTTGTAGA+AGG + chr3.4:74597554-74597573 MS.gene022304:intron 25.0%
!!! ATTTTGTTGGTTTTATGGAT+GGG + chr3.4:74597617-74597636 MS.gene022304:intron 25.0%
!!! GTTCTGAGTTAATAAGTTTT+AGG + chr3.4:74596627-74596646 MS.gene022304:intron 25.0%
!!! TATTTTGTTGGTTTTATGGA+TGG + chr3.4:74597616-74597635 MS.gene022304:intron 25.0%
!!! TCTATTTTAAGAGACTTTAG+GGG + chr3.4:74597312-74597331 MS.gene022304:intron 25.0%
!!! TTCATGTATTGGTTTGTTAT+AGG + chr3.4:74596221-74596240 MS.gene022304:intron 25.0%
AAACACTTGTGACACTATTT+GGG - chr3.4:74597873-74597892 None:intergenic 30.0%
AAATAGTGTCACAAGTGTTT+AGG + chr3.4:74597873-74597892 MS.gene022304:intron 30.0%
AGAAAGAACAAATACCTTGT+GGG + chr3.4:74596987-74597006 MS.gene022304:intron 30.0%
ATACATTTACGATAACCTCA+GGG - chr3.4:74596671-74596690 None:intergenic 30.0%
ATATTGTTTGCACTTGTGAT+TGG + chr3.4:74596539-74596558 MS.gene022304:intron 30.0%
GCAAAAAAGAACAAAACCAA+GGG - chr3.4:74597935-74597954 None:intergenic 30.0%
GTAAGATTAAATCCTACAGA+CGG - chr3.4:74598123-74598142 None:intergenic 30.0%
GTCATTTAAAGAGATCTCAA+GGG + chr3.4:74597374-74597393 MS.gene022304:intron 30.0%
TAAACACTTGTGACACTATT+TGG - chr3.4:74597874-74597893 None:intergenic 30.0%
TAAGATTAAATCCTACAGAC+GGG - chr3.4:74598122-74598141 None:intergenic 30.0%
TATTGTTTGCACTTGTGATT+GGG + chr3.4:74596540-74596559 MS.gene022304:intron 30.0%
TCAACAATGGCTGAAATATT+GGG + chr3.4:74597184-74597203 MS.gene022304:intron 30.0%
TGCAAAAAAGAACAAAACCA+AGG - chr3.4:74597936-74597955 None:intergenic 30.0%
TGGAAATACGAAAAGGAATT+GGG - chr3.4:74596874-74596893 None:intergenic 30.0%
TTAAATACAATCTTCACCCA+AGG - chr3.4:74597224-74597243 None:intergenic 30.0%
TTCAACAATGGCTGAAATAT+TGG + chr3.4:74597183-74597202 MS.gene022304:intron 30.0%
TTGGAAATACGAAAAGGAAT+TGG - chr3.4:74596875-74596894 None:intergenic 30.0%
TTGTTCTTTCTGTATGAACA+GGG - chr3.4:74596978-74596997 None:intergenic 30.0%
TTTCATAAGCTATCCATGAT+AGG - chr3.4:74597800-74597819 None:intergenic 30.0%
TTTGTTCTTTCTGTATGAAC+AGG - chr3.4:74596979-74596998 None:intergenic 30.0%
! ATCTGAAACATGAGAATAAG+AGG + chr3.4:74597070-74597089 MS.gene022304:intron 30.0%
! GTAGAGCAAAGATATTTTGT+TGG + chr3.4:74597604-74597623 MS.gene022304:intron 30.0%
! TAAAAAAATAGAAGGGGTGT+TGG - chr3.4:74596694-74596713 None:intergenic 30.0%
! TCAGTAAATTAATTTGACGG+AGG - chr3.4:74596754-74596773 None:intergenic 30.0%
! TGTCATTTAAAGAGATCTCA+AGG + chr3.4:74597373-74597392 MS.gene022304:intron 30.0%
!! AAATCAAGGTTGATGAGAAT+GGG + chr3.4:74596123-74596142 MS.gene022304:CDS 30.0%
!! CTATTTTAAGAGACTTTAGG+GGG + chr3.4:74597313-74597332 MS.gene022304:intron 30.0%
!! CTTTTTAAGCACACCAAAAA+AGG + chr3.4:74597462-74597481 MS.gene022304:intron 30.0%
!! TACAATGTGTTCATTTTAGC+TGG - chr3.4:74596928-74596947 None:intergenic 30.0%
!! TGGTTTGTTATAGGAAATTC+AGG + chr3.4:74596230-74596249 MS.gene022304:intron 30.0%
!!! ATCTTTAACAGCTGCTTTTA+TGG - chr3.4:74598308-74598327 None:intergenic 30.0%
!!! TGCTGTTTTATAACCTATCA+TGG + chr3.4:74597784-74597803 MS.gene022304:intron 30.0%
!!! TTTTGTTGGTTTTATGGATG+GGG + chr3.4:74597618-74597637 MS.gene022304:intron 30.0%
AACATAGCAGCAAAAGATGA+TGG - chr3.4:74598019-74598038 None:intergenic 35.0%
AATGACATCCATAACAAGCA+TGG + chr3.4:74596593-74596612 MS.gene022304:intron 35.0%
AGGCCATTATTCTTTGATGA+AGG + chr3.4:74597106-74597125 MS.gene022304:intron 35.0%
ATGGGTAAAAGGAAAGCAAA+TGG + chr3.4:74596384-74596403 MS.gene022304:CDS 35.0%
ATTTAAAGAGATCTCAAGGG+AGG + chr3.4:74597377-74597396 MS.gene022304:intron 35.0%
CAGAAAGAACAAATACCTTG+TGG + chr3.4:74596986-74597005 MS.gene022304:intron 35.0%
CAGTACTTACGTGTTCTTAA+TGG - chr3.4:74596422-74596441 None:intergenic 35.0%
CATTAAGAACACGTAAGTAC+TGG + chr3.4:74596420-74596439 MS.gene022304:intron 35.0%
GAAAGAACAAATACCTTGTG+GGG + chr3.4:74596988-74597007 MS.gene022304:intron 35.0%
GATACATTTACGATAACCTC+AGG - chr3.4:74596672-74596691 None:intergenic 35.0%
GGCATGATTCATAAACCAAA+AGG - chr3.4:74598101-74598120 None:intergenic 35.0%
TACATTTACGATAACCTCAG+GGG - chr3.4:74596670-74596689 None:intergenic 35.0%
TGCAAATTTGTGTCTTTGTC+TGG + chr3.4:74596464-74596483 MS.gene022304:intron 35.0%
TGTCATGAAGCTTCAAATCA+AGG + chr3.4:74596109-74596128 MS.gene022304:CDS 35.0%
! TACTGTTTCCATGCTTGTTA+TGG - chr3.4:74596604-74596623 None:intergenic 35.0%
! TTTAGCTGGCTTAAACATGA+TGG - chr3.4:74596914-74596933 None:intergenic 35.0%
!! CAAATCAAGGTTGATGAGAA+TGG + chr3.4:74596122-74596141 MS.gene022304:CDS 35.0%
!! GCTTCTATGATTGGTAGTTT+TGG + chr3.4:74598174-74598193 MS.gene022304:intron 35.0%
!! TTTTGTAGAAGGTGGATTTC+TGG + chr3.4:74597565-74597584 MS.gene022304:intron 35.0%
!!! GTAGGTCGTTTTTCATGTAT+TGG + chr3.4:74596210-74596229 MS.gene022304:intron 35.0%
AAAAGGAAAGCAAATGGAGG+AGG + chr3.4:74596390-74596409 MS.gene022304:CDS 40.0%
ACAATGGCTGAAATATTGGG+TGG + chr3.4:74597187-74597206 MS.gene022304:intron 40.0%
ACATGAGAATAAGAGGTCTG+CGG + chr3.4:74597077-74597096 MS.gene022304:intron 40.0%
ACCATATTCTTCTGTGCTCT+AGG + chr3.4:74597507-74597526 MS.gene022304:intron 40.0%
ACTTGTGATTGGGTGAAGAA+TGG + chr3.4:74596550-74596569 MS.gene022304:intron 40.0%
AGAATCCTAATCGCTGGATT+TGG - chr3.4:74595969-74595988 None:intergenic 40.0%
ATGGATGGGGTTTGAAGATA+TGG + chr3.4:74597631-74597650 MS.gene022304:intron 40.0%
ATTGAGCATTTATCCCCACA+AGG - chr3.4:74597004-74597023 None:intergenic 40.0%
CATCGGATCATTCTTATCGA+AGG - chr3.4:74596051-74596070 None:intergenic 40.0%
CATGAAAAACGACCTACCAA+CGG - chr3.4:74596207-74596226 None:intergenic 40.0%
CGGCCTTCATCAAAGAATAA+TGG - chr3.4:74597112-74597131 None:intergenic 40.0%
CTTCGATAAGAATGATCCGA+TGG + chr3.4:74596049-74596068 MS.gene022304:CDS 40.0%
GAAGAATGGTTATGTGCTTG+AGG + chr3.4:74596564-74596583 MS.gene022304:intron 40.0%
GATAAGAATGATCCGATGGT+GGG + chr3.4:74596053-74596072 MS.gene022304:CDS 40.0%
GGTAAAAGGAAAGCAAATGG+AGG + chr3.4:74596387-74596406 MS.gene022304:CDS 40.0%
GGTGGTTGAGTATATACCTT+GGG + chr3.4:74597205-74597224 MS.gene022304:intron 40.0%
TAGAGCACAGAAGAATATGG+TGG - chr3.4:74597508-74597527 None:intergenic 40.0%
TATCGAAGGATCCAACATTC+CGG - chr3.4:74596037-74596056 None:intergenic 40.0%
TGGATGGGGTTTGAAGATAT+GGG + chr3.4:74597632-74597651 MS.gene022304:intron 40.0%
TGGCACCCAAATCATGTATT+TGG - chr3.4:74596894-74596913 None:intergenic 40.0%
TTTGACAGTGTCAAAGGCAA+GGG - chr3.4:74596731-74596750 None:intergenic 40.0%
! AGTTCATTGGGTATTGCCTT+TGG + chr3.4:74596805-74596824 MS.gene022304:intron 40.0%
! ATCGTTTTGCAGCTACTGAA+TGG + chr3.4:74596365-74596384 MS.gene022304:intron 40.0%
! CTGCTATGTTTTCAACCTTC+TGG + chr3.4:74598029-74598048 MS.gene022304:intron 40.0%
! TCGTTTTGCAGCTACTGAAT+GGG + chr3.4:74596366-74596385 MS.gene022304:intron 40.0%
! TTTTCCTTCTTTTCCCTTCC+AGG + chr3.4:74598071-74598090 MS.gene022304:intron 40.0%
!! GGTGTGCTTAAAAAGTGTTG+CGG - chr3.4:74597457-74597476 None:intergenic 40.0%
!! GTGTGCTTAAAAAGTGTTGC+GGG - chr3.4:74597456-74597475 None:intergenic 40.0%
!! TGTGCTTAAAAAGTGTTGCG+GGG - chr3.4:74597455-74597474 None:intergenic 40.0%
!! TTTTAAGAGACTTTAGGGGG+AGG + chr3.4:74597316-74597335 MS.gene022304:intron 40.0%
!!! CGCTTTAAGACTTTTGGTTG+TGG + chr3.4:74596161-74596180 MS.gene022304:CDS 40.0%
!!! GATGCTCGCTTTAAGACTTT+TGG + chr3.4:74596155-74596174 MS.gene022304:CDS 40.0%
!!! TAAGAGGTCTGCGGTTTTTT+AGG + chr3.4:74597086-74597105 MS.gene022304:intron 40.0%
AACAAAACCAAGGGCCTGTT+TGG - chr3.4:74597926-74597945 None:intergenic 45.0%
AGTTAATCCAAACAGGCCCT+TGG + chr3.4:74597916-74597935 MS.gene022304:intron 45.0%
ATCGAAGGATCCAACATTCC+GGG - chr3.4:74596036-74596055 None:intergenic 45.0%
ATGCTGCAGTGAAGCTTAAC+TGG - chr3.4:74598271-74598290 None:intergenic 45.0%
ATTTACGATAACCTCAGGGG+AGG - chr3.4:74596667-74596686 None:intergenic 45.0%
CATAAACCAAAAGGTCCTCC+TGG - chr3.4:74598092-74598111 None:intergenic 45.0%
CGATAAGAATGATCCGATGG+TGG + chr3.4:74596052-74596071 MS.gene022304:CDS 45.0%
CGCAAAGAAACTCGTGCAAA+CGG + chr3.4:74595929-74595948 MS.gene022304:CDS 45.0%
CTTCATGACATCACCACAAG+CGG - chr3.4:74596099-74596118 None:intergenic 45.0%
GCAGCTACTGAATGGGTAAA+AGG + chr3.4:74596373-74596392 MS.gene022304:intron 45.0%
GCCTAGAGCACAGAAGAATA+TGG - chr3.4:74597511-74597530 None:intergenic 45.0%
GGGTGGTTGAGTATATACCT+TGG + chr3.4:74597204-74597223 MS.gene022304:intron 45.0%
GTTTGACAGTGTCAAAGGCA+AGG - chr3.4:74596732-74596751 None:intergenic 45.0%
TATGAATCATGCCCGTCTGT+AGG + chr3.4:74598108-74598127 MS.gene022304:intron 45.0%
TTATGAAGCTAAGCGTGCCT+CGG + chr3.4:74598327-74598346 MS.gene022304:CDS 45.0%
TTCATGACATCACCACAAGC+GGG - chr3.4:74596098-74596117 None:intergenic 45.0%
TTGAAGATATGGGCCCTGTT+TGG + chr3.4:74597642-74597661 MS.gene022304:intron 45.0%
!! TAAAAAGTGTTGCGGGGCAT+CGG - chr3.4:74597449-74597468 None:intergenic 45.0%
AACCAAAAGGTCCTCCTGGA+AGG - chr3.4:74598088-74598107 None:intergenic 50.0%
AATTACACTCACCTCCCCTG+AGG + chr3.4:74596653-74596672 MS.gene022304:intron 50.0%
ACAAGACCAGTTCCCACCAT+CGG - chr3.4:74596068-74596087 None:intergenic 50.0%
ACCAAAAGGTCCTCCTGGAA+GGG - chr3.4:74598087-74598106 None:intergenic 50.0%
AGACGCCAAATCCAGCGATT+AGG + chr3.4:74595961-74595980 MS.gene022304:CDS 50.0%
CGGAGGTTTGACAGTGTCAA+AGG - chr3.4:74596737-74596756 None:intergenic 50.0%
CGTGGAAGAATCCTAATCGC+TGG - chr3.4:74595975-74595994 None:intergenic 50.0%
CTGCAGTGAAGCTTAACTGG+CGG - chr3.4:74598268-74598287 None:intergenic 50.0%
GCTATTGCTTCATCGTCCGT+TGG + chr3.4:74596188-74596207 MS.gene022304:CDS 50.0%
TACGACAATCCCCGGAATGT+TGG + chr3.4:74596023-74596042 MS.gene022304:CDS 50.0%
TCCACGATTCTACCACGAGA+GGG + chr3.4:74595989-74596008 MS.gene022304:CDS 50.0%
TCCTCCTGGAAGGGAAAAGA+AGG - chr3.4:74598078-74598097 None:intergenic 50.0%
TCGAAGGATCCAACATTCCG+GGG - chr3.4:74596035-74596054 None:intergenic 50.0%
TGCAGTGAAGCTTAACTGGC+GGG - chr3.4:74598267-74598286 None:intergenic 50.0%
TGGATCACTACGACAATCCC+CGG + chr3.4:74596015-74596034 MS.gene022304:CDS 50.0%
TTCCACGATTCTACCACGAG+AGG + chr3.4:74595988-74596007 MS.gene022304:CDS 50.0%
TTGCTTCATCGTCCGTTGGT+AGG + chr3.4:74596192-74596211 MS.gene022304:intron 50.0%
! TCCTTCTTTTCCCTTCCAGG+AGG + chr3.4:74598074-74598093 MS.gene022304:intron 50.0%
!! AATGATCCGATGGTGGGAAC+TGG + chr3.4:74596059-74596078 MS.gene022304:CDS 50.0%
!! ATGGTGGGAACTGGTCTTGT+TGG + chr3.4:74596068-74596087 MS.gene022304:CDS 50.0%
ACCCTCTCGTGGTAGAATCG+TGG - chr3.4:74595993-74596012 None:intergenic 55.0%
ACGATTCTACCACGAGAGGG+TGG + chr3.4:74595992-74596011 MS.gene022304:CDS 55.0%
AGTGGCAGATTTGTCTCCCG+TGG - chr3.4:74598362-74598381 None:intergenic 55.0%
ATTCTACCACGAGAGGGTGG+TGG + chr3.4:74595995-74596014 MS.gene022304:CDS 55.0%
GACAGTGTCAAAGGCAAGGG+AGG - chr3.4:74596728-74596747 None:intergenic 55.0%
! TCACTGCAGCATGCTTGCTG+AGG + chr3.4:74598279-74598298 MS.gene022304:CDS 55.0%
! TCCCTTCCAGGAGGACCTTT+TGG + chr3.4:74598083-74598102 MS.gene022304:intron 55.0%
AGTGATCCACCACCCTCTCG+TGG - chr3.4:74596004-74596023 None:intergenic 60.0%
CTTGTTGGTGCACCCGCTTG+TGG + chr3.4:74596083-74596102 MS.gene022304:CDS 60.0%
TAAGCGTGCCTCGGCTACTG+CGG + chr3.4:74598336-74598355 MS.gene022304:CDS 60.0%
TGCCTCGGCTACTGCGGCCA+CGG + chr3.4:74598342-74598361 MS.gene022304:CDS 70.0%
GCCTCGGCTACTGCGGCCAC+GGG + chr3.4:74598343-74598362 MS.gene022304:CDS 75.0%
TCCCGTGGCCGCAGTAGCCG+AGG - chr3.4:74598347-74598366 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 74595918 74598387 74595918 ID=MS.gene022304
chr3.4 mRNA 74595918 74598387 74595918 ID=MS.gene022304.t1;Parent=MS.gene022304
chr3.4 exon 74595918 74596209 74595918 ID=MS.gene022304.t1.exon1;Parent=MS.gene022304.t1
chr3.4 CDS 74595918 74596209 74595918 ID=cds.MS.gene022304.t1;Parent=MS.gene022304.t1
chr3.4 exon 74596377 74596431 74596377 ID=MS.gene022304.t1.exon2;Parent=MS.gene022304.t1
chr3.4 CDS 74596377 74596431 74596377 ID=cds.MS.gene022304.t1;Parent=MS.gene022304.t1
chr3.4 exon 74598240 74598387 74598240 ID=MS.gene022304.t1.exon3;Parent=MS.gene022304.t1
chr3.4 CDS 74598240 74598387 74598240 ID=cds.MS.gene022304.t1;Parent=MS.gene022304.t1
Gene Sequence

>MS.gene022304

ATGCTGAGAATCGCAAAGAAACTCGTGCAAACGGCGTCGTTTCAGACGCCAAATCCAGCGATTAGGATTCTTCCACGATTCTACCACGAGAGGGTGGTGGATCACTACGACAATCCCCGGAATGTTGGATCCTTCGATAAGAATGATCCGATGGTGGGAACTGGTCTTGTTGGTGCACCCGCTTGTGGTGATGTCATGAAGCTTCAAATCAAGGTTGATGAGAATGGGAAAATCGTTGATGCTCGCTTTAAGACTTTTGGTTGTGGTTCTGCTATTGCTTCATCGTCCGTTGGTAGGTCGTTTTTCATGTATTGGTTTGTTATAGGAAATTCAGGATTTTTTTTTTTTTAGGGTTTGTTGAATTTCAATTCCCTTTTTATTGAATTTGATTATTACGATTATGTTATCGTTAATTGTGCTATTAATTATGTTTCTAATTTGTTGTTTATCGTTTTGCAGCTACTGAATGGGTAAAAGGAAAGCAAATGGAGGAGGTTTTAACCATTAAGAACACGTAAGTACTGGTCTAAGAATTTTGTACTTTGTTGCAAATTTGTGTCTTTGTCTGGCTGTTGTTTGTGTAGTACAGTATTACTCTGTGATTTATTCGTTAGTTATAAGATATTGTTTGCACTTGTGATTGGGTGAAGAATGGTTATGTGCTTGAGGATATTGAATGACATCCATAACAAGCATGGAAACAGTAACAGTTCTGAGTTAATAAGTTTTAGGGAAAATTACACTCACCTCCCCTGAGGTTATCGTAAATGTATCCAACACCCCTTCTATTTTTTTAAATAGCACTAACCTCCCTTGCCTTTGACACTGTCAAACCTCCGTCAAATTAATTTACTGAAACAATTAACACTGTCAAATTATTGTGAATCAGTTCATTGGGTATTGCCTTTGGATTCCAATTTTTATATGAAAAATTGTTATTGTCAATTCTAATTCCCAATTCCTTTTCGTATTTCCAAATACATGATTTGGGTGCCATCATGTTTAAGCCAGCTAAAATGAACACATTGTATGCTACAACAATCAAACATATTCACTTCCCTGTTCATACAGAAAGAACAAATACCTTGTGGGGATAAATGCTCAATAAAAAGAGGATAATTAAAACACATTCATCTTTTATGCAGCATGTGTATCTGAAACATGAGAATAAGAGGTCTGCGGTTTTTTAGGCCATTATTCTTTGATGAAGGCCGAGAGAATTATGATAATAGAGATTTAAAATAAGGAAAAAAAAATTAATAGCTTTCAACAATGGCTGAAATATTGGGTGGTTGAGTATATACCTTGGGTGAAGATTGTATTTAACAATAGAAAAGTAGCAGTTCAGTTTTAACAGATCAATTAACGGTATTAACTTAGAAGGGATATGTTTATCTATTTTAAGAGACTTTAGGGGGAGGTTATTGTCTATTATAAAACTAGAGGGGATGTTTGTGTCATTTAAAGAGATCTCAAGGGAGGTCACTATAATTTTCCCTAAGTTTTATTATATTTTGGGTCGTGCTAACCGATGCCCCGCAACACTTTTTAAGCACACCAAAAAAGGAAATTGTTAACAAATAAGATCCACCATATTCTTCTGTGCTCTAGGCTTTACATTTCTTCTTGAAATTGATGGTATATGAATTTTGTAGAAGGTGGATTTCTGGCTAGTCAAGTTTAGTTGTAGAGCAAAGATATTTTGTTGGTTTTATGGATGGGGTTTGAAGATATGGGCCCTGTTTGGATAAAGAGTTTATTTTGCAACTTATAGCTTAAGCCATGATCATATAAGCACTTATTATGATTAAACTATTTCTATAACACAAGATAAAATAAAGTCAAGCTGTTTTCATATAATCTGTATGCTGTTTTATAACCTATCATGGATAGCTTATGAAAATAAGCTGAAAACAGCTTATGAACATGTCATAGCTGTTTTCACAAGTTCTCCCAAATAGTGTCACAAGTGTTTAGGTCAATAGATCATCTCAAATAAGTTAATCCAAACAGGCCCTTGGTTTTGTTCTTTTTTGCAATGTCGTCTTCTTGTCTCCTTTTTAATGATGACAAGTTGATGAATGATGAATCTAATAAGCCATCATCTTTTGCTGCTATGTTTTCAACCTTCTGGTTTCTTTTTTTCTTTCTTTTTTTCCTTCTTTTCCCTTCCAGGAGGACCTTTTGGTTTATGAATCATGCCCGTCTGTAGGATTTAATCTTACTACTAAATAATTTTATTTTATCTTTGTTAATGCTTCTATGATTGGTAGTTTTGGACTGTTTGTTAGTTTTTGAAGTGACAAAATTACTCTTGTGCAGTGAGATTGCAAAGCATCTTTCACTCCCGCCAGTTAAGCTTCACTGCAGCATGCTTGCTGAGGATGCCATAAAAGCAGCTGTTAAAGATTATGAAGCTAAGCGTGCCTCGGCTACTGCGGCCACGGGAGACAAATCTGCCACTGCATGA
Protein sequence

>MS.gene022304.t1

MLRIAKKLVQTASFQTPNPAIRILPRFYHERVVDHYDNPRNVGSFDKNDPMVGTGLVGAPACGDVMKLQIKVDENGKIVDARFKTFGCGSAIASSSVATEWVKGKQMEEVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRASATAATGDKSATA