Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83301.t1 | XP_003601257.1 | 84.1 | 164 | 25 | 1 | 1 | 163 | 1 | 164 | 1.10E-66 | 262.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83301.t1 | Q8LR34 | 73.9 | 138 | 34 | 2 | 28 | 163 | 34 | 171 | 1.6e-48 | 193.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83301.t1 | B7FHC6 | 84.1 | 164 | 25 | 1 | 1 | 163 | 1 | 164 | 8.2e-67 | 262.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene83301 | MS.gene29363 | PPI |
| MS.gene83301 | MS.gene009043 | PPI |
| MS.gene83301 | MS.gene022304 | PPI |
| MS.gene83301 | MS.gene29099 | PPI |
| MS.gene83301 | MS.gene009053 | PPI |
| MS.gene83301 | MS.gene25969 | PPI |
| MS.gene022304 | MS.gene83301 | PPI |
| MS.gene83301 | MS.gene29101 | PPI |
| MS.gene83301 | MS.gene29054 | PPI |
| MS.gene83301 | MS.gene35660 | PPI |
| MS.gene038024 | MS.gene83301 | PPI |
| MS.gene83301 | MS.gene61049 | PPI |
| MS.gene83301 | MS.gene069794 | PPI |
| MS.gene83301 | MS.gene031844 | PPI |
| MS.gene83301 | MS.gene94805 | PPI |
| MS.gene30769 | MS.gene83301 | PPI |
| MS.gene83301 | MS.gene70632 | PPI |
| MS.gene83301 | MS.gene038497 | PPI |
| MS.gene83301 | MS.gene29055 | PPI |
| MS.gene83301 | MS.gene24026 | PPI |
| MS.gene83301 | MS.gene031845 | PPI |
| MS.gene83301 | MS.gene059021 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83301.t1 | MTR_3g077720 | 84.146 | 164 | 25 | 1 | 1 | 163 | 1 | 164 | 2.74e-98 | 280 |
| MS.gene83301.t1 | MTR_3g077730 | 78.626 | 131 | 25 | 2 | 32 | 159 | 5 | 135 | 1.98e-67 | 202 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83301.t1 | AT4G22220 | 73.485 | 132 | 33 | 2 | 26 | 155 | 27 | 158 | 1.15e-65 | 198 |
| MS.gene83301.t1 | AT4G04080 | 60.510 | 157 | 61 | 1 | 1 | 156 | 1 | 157 | 1.12e-63 | 193 |
| MS.gene83301.t1 | AT3G01020 | 59.146 | 164 | 63 | 2 | 1 | 163 | 3 | 163 | 2.41e-60 | 184 |
Find 47 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAATTAAGGTTGATGATAA+TGG | 0.228001 | 6.1:-62921031 | MS.gene83301:CDS |
| TGTCATGAAGTTTCAAATTA+AGG | 0.237273 | 6.1:-62921044 | MS.gene83301:CDS |
| AGCTGCCAATTCAAGCAATT+AGG | 0.287621 | 6.1:-62921189 | MS.gene83301:CDS |
| CCGAAGATCTTTCTTATTGA+AGG | 0.314287 | 6.1:+62921102 | None:intergenic |
| GATTCTTGCTTTAAGACCTT+TGG | 0.331092 | 6.1:-62920998 | MS.gene83301:CDS |
| GGTAAAGGGAAAGCAAATTA+AGG | 0.338517 | 6.1:-62920788 | MS.gene83301:CDS |
| ATGCTGCAGTGAAGCTTAAC+TGG | 0.354352 | 6.1:+62920599 | None:intergenic |
| GCTGCCAATTCAAGCAATTA+GGG | 0.356677 | 6.1:-62921188 | MS.gene83301:CDS |
| AGAACCCTAATTGCTTGAAT+TGG | 0.366684 | 6.1:+62921184 | None:intergenic |
| CGCAAAGAAACTCGTGCAAA+CGG | 0.368310 | 6.1:-62921221 | MS.gene83301:CDS |
| CTTCAATAAGAAAGATCTTC+GGG | 0.392132 | 6.1:-62921101 | MS.gene83301:CDS |
| GCAAAGAAACTCGTGCAAAC+GGG | 0.401810 | 6.1:-62921220 | MS.gene83301:CDS |
| ATTGAAGGATCCAACGTTCC+GGG | 0.412834 | 6.1:+62921117 | None:intergenic |
| TATTGAAGGATCCAACGTTC+CGG | 0.415466 | 6.1:+62921116 | None:intergenic |
| GCAGCTACTGAATGGGTAAA+GGG | 0.419402 | 6.1:-62920802 | MS.gene83301:intron |
| TGCCTCGGCTACTGCGGCCA+CGG | 0.446467 | 6.1:-62920525 | MS.gene83301:CDS |
| CCTTCAATAAGAAAGATCTT+CGG | 0.452805 | 6.1:-62921102 | MS.gene83301:CDS |
| TGCTTTAAGACCTTTGGATG+TGG | 0.461939 | 6.1:-62920992 | MS.gene83301:CDS |
| TGCAGCTACTGAATGGGTAA+AGG | 0.478916 | 6.1:-62920803 | MS.gene83301:intron |
| CTTCGGGAGGTGGGATCTGT+TGG | 0.484742 | 6.1:-62921085 | MS.gene83301:CDS |
| TAAGAAAGATCTTCGGGAGG+TGG | 0.491830 | 6.1:-62921095 | MS.gene83301:CDS |
| GCCTCGGCTACTGCGGCCAC+GGG | 0.517239 | 6.1:-62920524 | MS.gene83301:CDS |
| TGCAGTGAAGCTTAACTGGC+GGG | 0.542392 | 6.1:+62920603 | None:intergenic |
| TCCCGTGGCCGCAGTAGCCG+AGG | 0.550070 | 6.1:+62920523 | None:intergenic |
| TACAACAATCCCCGGAACGT+TGG | 0.563672 | 6.1:-62921127 | MS.gene83301:CDS |
| AAGAAAGATCTTCGGGAGGT+GGG | 0.571174 | 6.1:-62921094 | MS.gene83301:CDS |
| TTATGAAGCTAAGCGTGCCT+CGG | 0.575006 | 6.1:-62920540 | MS.gene83301:CDS |
| CTTCATGACATCACCACAAG+CGG | 0.586806 | 6.1:+62921057 | None:intergenic |
| TTCCACGATTCTACAACAAG+AGG | 0.593896 | 6.1:-62921162 | MS.gene83301:CDS |
| TTGAAGGATCCAACGTTCCG+GGG | 0.597622 | 6.1:+62921118 | None:intergenic |
| TAAGCGTGCCTCGGCTACTG+CGG | 0.598393 | 6.1:-62920531 | MS.gene83301:CDS |
| CAAAGAAACTCGTGCAAACG+GGG | 0.599049 | 6.1:-62921219 | MS.gene83301:CDS |
| TTCATGACATCACCACAAGC+GGG | 0.599308 | 6.1:+62921058 | None:intergenic |
| TGGATCACTACAACAATCCC+CGG | 0.601326 | 6.1:-62921135 | MS.gene83301:CDS |
| ACCCTCTTGTTGTAGAATCG+TGG | 0.607283 | 6.1:+62921160 | None:intergenic |
| TCACTGCAGCATGCTTGCTG+AGG | 0.608636 | 6.1:-62920588 | MS.gene83301:CDS |
| TCTGTTGGTGCACCCGCTTG+TGG | 0.618008 | 6.1:-62921070 | MS.gene83301:CDS |
| ATTCTACAACAAGAGGGTGG+TGG | 0.622053 | 6.1:-62921155 | MS.gene83301:CDS |
| AGTGGCAGATTTGTCTCCCG+TGG | 0.628353 | 6.1:+62920508 | None:intergenic |
| CTGCAGTGAAGCTTAACTGG+CGG | 0.631259 | 6.1:+62920602 | None:intergenic |
| AATAGCGGAACCACATCCAA+AGG | 0.639771 | 6.1:+62920982 | None:intergenic |
| TCCACGATTCTACAACAAGA+GGG | 0.643893 | 6.1:-62921161 | MS.gene83301:CDS |
| CAATGGGAGAAATCATGCAG+TGG | 0.672265 | 6.1:+62920490 | None:intergenic |
| AACGGACGATGAAGCAATAG+CGG | 0.680554 | 6.1:+62920967 | None:intergenic |
| TGAGGATGCTATAAAAGCAG+CGG | 0.695297 | 6.1:-62920570 | MS.gene83301:CDS |
| CAATAAGAAAGATCTTCGGG+AGG | 0.711849 | 6.1:-62921098 | MS.gene83301:CDS |
| ACGATTCTACAACAAGAGGG+TGG | 0.727989 | 6.1:-62921158 | MS.gene83301:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTAAGGATTTTTTTTTA+GGG | - | chr6.1:62920826-62920845 | MS.gene83301:intron | 10.0% |
| !!! | GAAATTAAGGATTTTTTTTT+AGG | - | chr6.1:62920825-62920844 | MS.gene83301:intron | 15.0% |
| ! | ATGAAAAAACAACCTACTAA+CGG | + | chr6.1:62920788-62920807 | None:intergenic | 25.0% |
| ! | GAATTTGATTATTACGACTA+TGG | - | chr6.1:62920880-62920899 | MS.gene83301:intron | 25.0% |
| ! | TGTCATGAAGTTTCAAATTA+AGG | - | chr6.1:62920690-62920709 | MS.gene83301:intron | 25.0% |
| !!! | CAAATTAAGGTTGATGATAA+TGG | - | chr6.1:62920703-62920722 | MS.gene83301:intron | 25.0% |
| !!! | CTACTAAACAGTTTTATCTT+TGG | - | chr6.1:62921032-62921051 | MS.gene83301:CDS | 25.0% |
| !!! | TAGGTTGTTTTTTCATGTAT+TGG | - | chr6.1:62920792-62920811 | MS.gene83301:CDS | 25.0% |
| !!! | TGGTTTGTTATAGGAAATTA+AGG | - | chr6.1:62920812-62920831 | MS.gene83301:intron | 25.0% |
| !!! | TTCATGTATTGGTTTGTTAT+AGG | - | chr6.1:62920803-62920822 | MS.gene83301:intron | 25.0% |
| ACTTCAAAAACTACCAATCA+TGG | + | chr6.1:62921066-62921085 | None:intergenic | 30.0% | |
| ATTAAGAACACGTAAGTAAC+TGG | - | chr6.1:62920980-62920999 | MS.gene83301:CDS | 30.0% | |
| CCTTCAATAAGAAAGATCTT+CGG | - | chr6.1:62920632-62920651 | MS.gene83301:intron | 30.0% | |
| CTTCAATAAGAAAGATCTTC+GGG | - | chr6.1:62920633-62920652 | MS.gene83301:intron | 30.0% | |
| AGAACCCTAATTGCTTGAAT+TGG | + | chr6.1:62920553-62920572 | None:intergenic | 35.0% | |
| CCGAAGATCTTTCTTATTGA+AGG | + | chr6.1:62920635-62920654 | None:intergenic | 35.0% | |
| GGTAAAGGGAAAGCAAATTA+AGG | - | chr6.1:62920946-62920965 | MS.gene83301:intron | 35.0% | |
| !! | GATTCTTGCTTTAAGACCTT+TGG | - | chr6.1:62920736-62920755 | MS.gene83301:intron | 35.0% |
| !! | TTTGGTAATGCTTCCATGAT+TGG | - | chr6.1:62921050-62921069 | MS.gene83301:CDS | 35.0% |
| AGCTGCCAATTCAAGCAATT+AGG | - | chr6.1:62920545-62920564 | MS.gene83301:CDS | 40.0% | |
| CAATAAGAAAGATCTTCGGG+AGG | - | chr6.1:62920636-62920655 | MS.gene83301:intron | 40.0% | |
| GCTGCCAATTCAAGCAATTA+GGG | - | chr6.1:62920546-62920565 | MS.gene83301:CDS | 40.0% | |
| TATTGAAGGATCCAACGTTC+CGG | + | chr6.1:62920621-62920640 | None:intergenic | 40.0% | |
| TCCACGATTCTACAACAAGA+GGG | - | chr6.1:62920573-62920592 | MS.gene83301:CDS | 40.0% | |
| TGAGGATGCTATAAAAGCAG+CGG | - | chr6.1:62921164-62921183 | MS.gene83301:CDS | 40.0% | |
| TTCCACGATTCTACAACAAG+AGG | - | chr6.1:62920572-62920591 | MS.gene83301:CDS | 40.0% | |
| ! | ATCGTTTTGCAGCTACTGAA+TGG | - | chr6.1:62920924-62920943 | MS.gene83301:intron | 40.0% |
| ! | TCGTTTTGCAGCTACTGAAT+GGG | - | chr6.1:62920925-62920944 | MS.gene83301:intron | 40.0% |
| !! | TGCTTTAAGACCTTTGGATG+TGG | - | chr6.1:62920742-62920761 | MS.gene83301:intron | 40.0% |
| AACGGACGATGAAGCAATAG+CGG | + | chr6.1:62920770-62920789 | None:intergenic | 45.0% | |
| AAGAAAGATCTTCGGGAGGT+GGG | - | chr6.1:62920640-62920659 | MS.gene83301:intron | 45.0% | |
| ACGATTCTACAACAAGAGGG+TGG | - | chr6.1:62920576-62920595 | MS.gene83301:CDS | 45.0% | |
| ATGCTGCAGTGAAGCTTAAC+TGG | + | chr6.1:62921138-62921157 | None:intergenic | 45.0% | |
| ATTCTACAACAAGAGGGTGG+TGG | - | chr6.1:62920579-62920598 | MS.gene83301:CDS | 45.0% | |
| ATTGAAGGATCCAACGTTCC+GGG | + | chr6.1:62920620-62920639 | None:intergenic | 45.0% | |
| CAAAGAAACTCGTGCAAACG+GGG | - | chr6.1:62920515-62920534 | MS.gene83301:CDS | 45.0% | |
| CGCAAAGAAACTCGTGCAAA+CGG | - | chr6.1:62920513-62920532 | MS.gene83301:CDS | 45.0% | |
| CTTCATGACATCACCACAAG+CGG | + | chr6.1:62920680-62920699 | None:intergenic | 45.0% | |
| GCAAAGAAACTCGTGCAAAC+GGG | - | chr6.1:62920514-62920533 | MS.gene83301:CDS | 45.0% | |
| GCAGCTACTGAATGGGTAAA+GGG | - | chr6.1:62920932-62920951 | MS.gene83301:intron | 45.0% | |
| TAAGAAAGATCTTCGGGAGG+TGG | - | chr6.1:62920639-62920658 | MS.gene83301:intron | 45.0% | |
| TGCAGCTACTGAATGGGTAA+AGG | - | chr6.1:62920931-62920950 | MS.gene83301:intron | 45.0% | |
| TGGATCACTACAACAATCCC+CGG | - | chr6.1:62920599-62920618 | MS.gene83301:CDS | 45.0% | |
| TTATGAAGCTAAGCGTGCCT+CGG | - | chr6.1:62921194-62921213 | MS.gene83301:CDS | 45.0% | |
| TTCATGACATCACCACAAGC+GGG | + | chr6.1:62920679-62920698 | None:intergenic | 45.0% | |
| TTGCTTCATCGTCCGTTAGT+AGG | - | chr6.1:62920773-62920792 | MS.gene83301:CDS | 45.0% | |
| ! | AATAGCGGAACCACATCCAA+AGG | + | chr6.1:62920755-62920774 | None:intergenic | 45.0% |
| ! | ACCCTCTTGTTGTAGAATCG+TGG | + | chr6.1:62920577-62920596 | None:intergenic | 45.0% |
| CTGCAGTGAAGCTTAACTGG+CGG | + | chr6.1:62921135-62921154 | None:intergenic | 50.0% | |
| TACAACAATCCCCGGAACGT+TGG | - | chr6.1:62920607-62920626 | MS.gene83301:CDS | 50.0% | |
| TGCAGTGAAGCTTAACTGGC+GGG | + | chr6.1:62921134-62921153 | None:intergenic | 50.0% | |
| TTGAAGGATCCAACGTTCCG+GGG | + | chr6.1:62920619-62920638 | None:intergenic | 50.0% | |
| AGTGGCAGATTTGTCTCCCG+TGG | + | chr6.1:62921229-62921248 | None:intergenic | 55.0% | |
| ! | TCACTGCAGCATGCTTGCTG+AGG | - | chr6.1:62921146-62921165 | MS.gene83301:CDS | 55.0% |
| CTTCGGGAGGTGGGATCTGT+TGG | - | chr6.1:62920649-62920668 | MS.gene83301:intron | 60.0% | |
| TAAGCGTGCCTCGGCTACTG+CGG | - | chr6.1:62921203-62921222 | MS.gene83301:CDS | 60.0% | |
| TCTGTTGGTGCACCCGCTTG+TGG | - | chr6.1:62920664-62920683 | MS.gene83301:intron | 60.0% | |
| TGCCTCGGCTACTGCGGCCA+CGG | - | chr6.1:62921209-62921228 | MS.gene83301:CDS | 70.0% | |
| GCCTCGGCTACTGCGGCCAC+GGG | - | chr6.1:62921210-62921229 | MS.gene83301:CDS | 75.0% | |
| TCCCGTGGCCGCAGTAGCCG+AGG | + | chr6.1:62921214-62921233 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 62920502 | 62921254 | 62920502 | ID=MS.gene83301 |
| chr6.1 | mRNA | 62920502 | 62921254 | 62920502 | ID=MS.gene83301.t1;Parent=MS.gene83301 |
| chr6.1 | exon | 62920966 | 62921254 | 62920966 | ID=MS.gene83301.t1.exon1;Parent=MS.gene83301.t1 |
| chr6.1 | CDS | 62920966 | 62921254 | 62920966 | ID=cds.MS.gene83301.t1;Parent=MS.gene83301.t1 |
| chr6.1 | exon | 62920766 | 62920820 | 62920766 | ID=MS.gene83301.t1.exon2;Parent=MS.gene83301.t1 |
| chr6.1 | CDS | 62920766 | 62920820 | 62920766 | ID=cds.MS.gene83301.t1;Parent=MS.gene83301.t1 |
| chr6.1 | exon | 62920502 | 62920649 | 62920502 | ID=MS.gene83301.t1.exon3;Parent=MS.gene83301.t1 |
| chr6.1 | CDS | 62920502 | 62920649 | 62920502 | ID=cds.MS.gene83301.t1;Parent=MS.gene83301.t1 |
| Gene Sequence |
| Protein sequence |
