Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022326.t1 | XP_013461973.1 | 97.9 | 97 | 2 | 0 | 1 | 97 | 1 | 97 | 1.90E-45 | 191.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022326.t1 | P34893 | 73.2 | 97 | 26 | 0 | 1 | 97 | 1 | 97 | 8.2e-37 | 154.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022326.t1 | G7ZWL8 | 97.9 | 97 | 2 | 0 | 1 | 97 | 1 | 97 | 1.4e-45 | 191.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene022326 | MS.gene66497 | PPI |
| MS.gene022326 | MS.gene92186 | PPI |
| MS.gene022326 | MS.gene005697 | PPI |
| MS.gene022326 | MS.gene21357 | PPI |
| MS.gene022326 | MS.gene66498 | PPI |
| MS.gene022326 | MS.gene006641 | PPI |
| MS.gene022326 | MS.gene24509 | PPI |
| MS.gene022326 | MS.gene074000 | PPI |
| MS.gene022326 | MS.gene21355 | PPI |
| MS.gene022326 | MS.gene005698 | PPI |
| MS.gene022326 | MS.gene24508 | PPI |
| MS.gene022326 | MS.gene00537 | PPI |
| MS.gene022326 | MS.gene00538 | PPI |
| MS.gene022326 | MS.gene031195 | PPI |
| MS.gene022326 | MS.gene21583 | PPI |
| MS.gene022326 | MS.gene25990 | PPI |
| MS.gene022326 | MS.gene051662 | PPI |
| MS.gene022326 | MS.gene21581 | PPI |
| MS.gene022326 | MS.gene40328 | PPI |
| MS.gene022326 | MS.gene031197 | PPI |
| MS.gene022326 | MS.gene051663 | PPI |
| MS.gene022326 | MS.gene29382 | PPI |
| MS.gene022326 | MS.gene039040 | PPI |
| MS.gene022326 | MS.gene014672 | PPI |
| MS.gene022326 | MS.gene75319 | PPI |
| MS.gene022326 | MS.gene045566 | PPI |
| MS.gene022326 | MS.gene70655 | PPI |
| MS.gene022326 | MS.gene75317 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022326.t1 | MTR_3g110030 | 97.938 | 97 | 2 | 0 | 1 | 97 | 1 | 97 | 4.32e-65 | 191 |
| MS.gene022326.t1 | MTR_3g077350 | 87.059 | 85 | 11 | 0 | 1 | 85 | 1 | 85 | 4.02e-49 | 150 |
| MS.gene022326.t1 | MTR_7g089300 | 74.227 | 97 | 25 | 0 | 1 | 97 | 1 | 97 | 6.32e-48 | 148 |
| MS.gene022326.t1 | MTR_3g089960 | 85.714 | 42 | 6 | 0 | 56 | 97 | 212 | 253 | 1.86e-19 | 79.7 |
| MS.gene022326.t1 | MTR_3g077580 | 89.189 | 37 | 4 | 0 | 1 | 37 | 1 | 37 | 7.91e-17 | 69.3 |
| MS.gene022326.t1 | MTR_2g043230 | 40.000 | 95 | 55 | 2 | 3 | 95 | 159 | 253 | 4.68e-12 | 60.1 |
| MS.gene022326.t1 | MTR_4g124910 | 38.947 | 95 | 56 | 2 | 3 | 95 | 158 | 252 | 2.25e-11 | 58.2 |
| MS.gene022326.t1 | MTR_4g124910 | 37.778 | 90 | 54 | 2 | 7 | 95 | 64 | 152 | 4.05e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022326.t1 | AT1G23100 | 74.227 | 97 | 25 | 0 | 1 | 97 | 1 | 97 | 3.53e-51 | 156 |
| MS.gene022326.t1 | AT1G14980 | 73.196 | 97 | 26 | 0 | 1 | 97 | 1 | 97 | 2.74e-50 | 154 |
| MS.gene022326.t1 | AT1G14980 | 73.171 | 82 | 22 | 0 | 1 | 82 | 1 | 82 | 2.59e-40 | 128 |
| MS.gene022326.t1 | AT5G20720 | 37.895 | 95 | 57 | 2 | 3 | 95 | 158 | 252 | 8.60e-12 | 59.3 |
| MS.gene022326.t1 | AT5G20720 | 37.895 | 95 | 57 | 2 | 3 | 95 | 158 | 252 | 8.60e-12 | 59.3 |
| MS.gene022326.t1 | AT5G20720 | 37.895 | 95 | 57 | 2 | 3 | 95 | 158 | 252 | 8.60e-12 | 59.3 |
| MS.gene022326.t1 | AT5G20720 | 37.895 | 95 | 57 | 2 | 3 | 95 | 158 | 252 | 8.60e-12 | 59.3 |
Find 39 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGTCATCAAGCTTAATT+TGG | 0.119195 | 3.4:+74256701 | None:intergenic |
| GTTTAGGGATGAGGACATTT+TGG | 0.181417 | 3.4:-74256553 | MS.gene022326:CDS |
| TTTGCAGGTTCCATTTGTTT+AGG | 0.240342 | 3.4:-74256569 | MS.gene022326:intron |
| AAGACTTCCCAGCTTAATTC+TGG | 0.252049 | 3.4:-74256829 | MS.gene022326:CDS |
| TTGCAGGTTCCATTTGTTTA+GGG | 0.300222 | 3.4:-74256568 | MS.gene022326:intron |
| CCCGGGTCGCGAGACAAATC+TGG | 0.306875 | 3.4:-74256787 | MS.gene022326:CDS |
| CACGTTCTCTTGCCGGAATA+TGG | 0.349479 | 3.4:-74256730 | MS.gene022326:CDS |
| ATTTGGGAGCCACCATATTC+CGG | 0.363161 | 3.4:+74256718 | None:intergenic |
| GAGAATACCGGCAGAGGTCT+TGG | 0.370880 | 3.4:+74256861 | None:intergenic |
| TTCGACGAGGATGCGGTTGA+AGG | 0.399797 | 3.4:+74256900 | None:intergenic |
| TTAAGCTGGGAAGTCTTCTC+TGG | 0.400010 | 3.4:+74256835 | None:intergenic |
| TTTGTCATCAAGCTTAATTT+GGG | 0.416340 | 3.4:+74256702 | None:intergenic |
| AGGTGATCACGTTCTCTTGC+CGG | 0.428049 | 3.4:-74256737 | MS.gene022326:CDS |
| CCCAGATTTGTCTCGCGACC+CGG | 0.430265 | 3.4:+74256786 | None:intergenic |
| TGTCCTCATCCCTAAACAAA+TGG | 0.441581 | 3.4:+74256559 | None:intergenic |
| GTTCCATTTGTTTAGGGATG+AGG | 0.449740 | 3.4:-74256562 | MS.gene022326:CDS |
| ACGAGGATGCGGTTGAAGGT+TGG | 0.451370 | 3.4:+74256904 | None:intergenic |
| AAGGTGGTAGCTGTTGGTCC+CGG | 0.456091 | 3.4:-74256805 | MS.gene022326:CDS |
| CCACCTTTCCAGAATTAAGC+TGG | 0.456517 | 3.4:+74256821 | None:intergenic |
| TGGGCATTCTGCGTGATTGA+CGG | 0.466133 | 3.4:-74256533 | None:intergenic |
| CTTCTCTGGGAGGAGAATAC+CGG | 0.509700 | 3.4:+74256849 | None:intergenic |
| CCTCCTTCCAAGACCTCTGC+CGG | 0.525221 | 3.4:-74256868 | MS.gene022326:CDS |
| AGGTGGTAGCTGTTGGTCCC+GGG | 0.533844 | 3.4:-74256804 | MS.gene022326:CDS |
| CACCTTTCCAGAATTAAGCT+GGG | 0.554181 | 3.4:+74256822 | None:intergenic |
| CCGGGTCGCGAGACAAATCT+GGG | 0.555950 | 3.4:-74256786 | MS.gene022326:CDS |
| TTTGATTCCATTGTCTGTCA+AGG | 0.556707 | 3.4:-74256761 | MS.gene022326:CDS |
| ATTCCATTGTCTGTCAAGGA+AGG | 0.559362 | 3.4:-74256757 | MS.gene022326:CDS |
| CGGCAGAGGTCTTGGAAGGA+GGG | 0.568131 | 3.4:+74256869 | None:intergenic |
| CCGGCAGAGGTCTTGGAAGG+AGG | 0.571190 | 3.4:+74256868 | None:intergenic |
| TTCCCAGCTTAATTCTGGAA+AGG | 0.575103 | 3.4:-74256824 | MS.gene022326:CDS |
| TCACCTTCCTTGACAGACAA+TGG | 0.578277 | 3.4:+74256754 | None:intergenic |
| TCTGGAAAGGTGGTAGCTGT+TGG | 0.588174 | 3.4:-74256811 | MS.gene022326:CDS |
| CCAGCTTAATTCTGGAAAGG+TGG | 0.590212 | 3.4:-74256821 | MS.gene022326:CDS |
| CCAGATTTGTCTCGCGACCC+GGG | 0.593410 | 3.4:+74256787 | None:intergenic |
| TGGGAGGAGAATACCGGCAG+AGG | 0.603629 | 3.4:+74256855 | None:intergenic |
| TAAGCTGGGAAGTCTTCTCT+GGG | 0.609151 | 3.4:+74256836 | None:intergenic |
| GTTCTCTTGCCGGAATATGG+TGG | 0.611791 | 3.4:-74256727 | MS.gene022326:CDS |
| ATACCGGCAGAGGTCTTGGA+AGG | 0.632905 | 3.4:+74256865 | None:intergenic |
| GCTGGGAAGTCTTCTCTGGG+AGG | 0.712584 | 3.4:+74256839 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGCAATTATATTTACAATT+TGG | - | chr3.4:74256849-74256868 | MS.gene022326:CDS | 15.0% |
| !!! | TTTCTTTGTTTTATATTTGC+AGG | - | chr3.4:74256874-74256893 | MS.gene022326:CDS | 20.0% |
| ! | CATAAAAAGAAACACATTCA+AGG | + | chr3.4:74256811-74256830 | None:intergenic | 25.0% |
| ! | TTTGTCATCAAGCTTAATTT+GGG | + | chr3.4:74256759-74256778 | None:intergenic | 25.0% |
| !! | TGAATGTGTTTCTTTTTATG+TGG | - | chr3.4:74256811-74256830 | MS.gene022326:CDS | 25.0% |
| CTTTGTCATCAAGCTTAATT+TGG | + | chr3.4:74256760-74256779 | None:intergenic | 30.0% | |
| ! | ATGACAAAGAGTATGTCATA+GGG | - | chr3.4:74256771-74256790 | MS.gene022326:CDS | 30.0% |
| TTGCAGGTTCCATTTGTTTA+GGG | - | chr3.4:74256890-74256909 | MS.gene022326:CDS | 35.0% | |
| TTTGATTCCATTGTCTGTCA+AGG | - | chr3.4:74256697-74256716 | MS.gene022326:intron | 35.0% | |
| TTTGCAGGTTCCATTTGTTT+AGG | - | chr3.4:74256889-74256908 | MS.gene022326:CDS | 35.0% | |
| ! | GATGACAAAGAGTATGTCAT+AGG | - | chr3.4:74256770-74256789 | MS.gene022326:CDS | 35.0% |
| ! | TTTAGGGATGAGGACATTTT+GGG | - | chr3.4:74256906-74256925 | MS.gene022326:CDS | 35.0% |
| !! | TTTTATGTGGACTGCTAACT+TGG | - | chr3.4:74256824-74256843 | MS.gene022326:CDS | 35.0% |
| AAGACTTCCCAGCTTAATTC+TGG | - | chr3.4:74256629-74256648 | MS.gene022326:intron | 40.0% | |
| ATTCCATTGTCTGTCAAGGA+AGG | - | chr3.4:74256701-74256720 | MS.gene022326:CDS | 40.0% | |
| CACCTTTCCAGAATTAAGCT+GGG | + | chr3.4:74256639-74256658 | None:intergenic | 40.0% | |
| GTTCCATTTGTTTAGGGATG+AGG | - | chr3.4:74256896-74256915 | MS.gene022326:CDS | 40.0% | |
| TGTCCTCATCCCTAAACAAA+TGG | + | chr3.4:74256902-74256921 | None:intergenic | 40.0% | |
| ! | GTTTAGGGATGAGGACATTT+TGG | - | chr3.4:74256905-74256924 | MS.gene022326:CDS | 40.0% |
| ! | TTCCCAGCTTAATTCTGGAA+AGG | - | chr3.4:74256634-74256653 | MS.gene022326:intron | 40.0% |
| !!! | CAATTTTTTCGACGAGGATG+CGG | + | chr3.4:74256568-74256587 | None:intergenic | 40.0% |
| ATTTGGGAGCCACCATATTC+CGG | + | chr3.4:74256743-74256762 | None:intergenic | 45.0% | |
| CCACCTTTCCAGAATTAAGC+TGG | + | chr3.4:74256640-74256659 | None:intergenic | 45.0% | |
| TAAGCTGGGAAGTCTTCTCT+GGG | + | chr3.4:74256625-74256644 | None:intergenic | 45.0% | |
| TCACCTTCCTTGACAGACAA+TGG | + | chr3.4:74256707-74256726 | None:intergenic | 45.0% | |
| TTAAGCTGGGAAGTCTTCTC+TGG | + | chr3.4:74256626-74256645 | None:intergenic | 45.0% | |
| ! | CCAGCTTAATTCTGGAAAGG+TGG | - | chr3.4:74256637-74256656 | MS.gene022326:intron | 45.0% |
| !!! | GAGGGACAATTTTTTCGACG+AGG | + | chr3.4:74256574-74256593 | None:intergenic | 45.0% |
| AGGTGATCACGTTCTCTTGC+CGG | - | chr3.4:74256721-74256740 | MS.gene022326:CDS | 50.0% | |
| CACGTTCTCTTGCCGGAATA+TGG | - | chr3.4:74256728-74256747 | MS.gene022326:CDS | 50.0% | |
| GTTCTCTTGCCGGAATATGG+TGG | - | chr3.4:74256731-74256750 | MS.gene022326:CDS | 50.0% | |
| TCTGGAAAGGTGGTAGCTGT+TGG | - | chr3.4:74256647-74256666 | MS.gene022326:intron | 50.0% | |
| ! | CTTCTCTGGGAGGAGAATAC+CGG | + | chr3.4:74256612-74256631 | None:intergenic | 50.0% |
| AAGGTGGTAGCTGTTGGTCC+CGG | - | chr3.4:74256653-74256672 | MS.gene022326:intron | 55.0% | |
| ACGAGGATGCGGTTGAAGGT+TGG | + | chr3.4:74256557-74256576 | None:intergenic | 55.0% | |
| ATACCGGCAGAGGTCTTGGA+AGG | + | chr3.4:74256596-74256615 | None:intergenic | 55.0% | |
| GAGAATACCGGCAGAGGTCT+TGG | + | chr3.4:74256600-74256619 | None:intergenic | 55.0% | |
| TTCGACGAGGATGCGGTTGA+AGG | + | chr3.4:74256561-74256580 | None:intergenic | 55.0% | |
| AGGTGGTAGCTGTTGGTCCC+GGG | - | chr3.4:74256654-74256673 | MS.gene022326:intron | 60.0% | |
| CCAGATTTGTCTCGCGACCC+GGG | + | chr3.4:74256674-74256693 | None:intergenic | 60.0% | |
| CCCAGATTTGTCTCGCGACC+CGG | + | chr3.4:74256675-74256694 | None:intergenic | 60.0% | |
| CCGGGTCGCGAGACAAATCT+GGG | - | chr3.4:74256672-74256691 | MS.gene022326:intron | 60.0% | |
| CCTCCTTCCAAGACCTCTGC+CGG | - | chr3.4:74256590-74256609 | MS.gene022326:intron | 60.0% | |
| CGGCAGAGGTCTTGGAAGGA+GGG | + | chr3.4:74256592-74256611 | None:intergenic | 60.0% | |
| GCTGGGAAGTCTTCTCTGGG+AGG | + | chr3.4:74256622-74256641 | None:intergenic | 60.0% | |
| TGGGAGGAGAATACCGGCAG+AGG | + | chr3.4:74256606-74256625 | None:intergenic | 60.0% | |
| CCCGGGTCGCGAGACAAATC+TGG | - | chr3.4:74256671-74256690 | MS.gene022326:intron | 65.0% | |
| CCGGCAGAGGTCTTGGAAGG+AGG | + | chr3.4:74256593-74256612 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 74256536 | 74256944 | 74256536 | ID=MS.gene022326 |
| chr3.4 | mRNA | 74256536 | 74256944 | 74256536 | ID=MS.gene022326.t1;Parent=MS.gene022326 |
| chr3.4 | exon | 74256700 | 74256944 | 74256700 | ID=MS.gene022326.t1.exon1;Parent=MS.gene022326.t1 |
| chr3.4 | CDS | 74256700 | 74256944 | 74256700 | ID=cds.MS.gene022326.t1;Parent=MS.gene022326.t1 |
| chr3.4 | exon | 74256536 | 74256584 | 74256536 | ID=MS.gene022326.t1.exon2;Parent=MS.gene022326.t1 |
| chr3.4 | CDS | 74256536 | 74256584 | 74256536 | ID=cds.MS.gene022326.t1;Parent=MS.gene022326.t1 |
| Gene Sequence |
| Protein sequence |
