Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70655.t1 | XP_004505282.1 | 96.9 | 97 | 3 | 0 | 1 | 97 | 1 | 97 | 2.10E-44 | 188 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70655.t1 | P34893 | 72.2 | 97 | 27 | 0 | 1 | 97 | 1 | 97 | 3.1e-36 | 152.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70655.t1 | A0A1S2YIG4 | 96.9 | 97 | 3 | 0 | 1 | 97 | 1 | 97 | 1.5e-44 | 188.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049666 | MS.gene70655 | 0.811902 | 5.55E-51 | -1.69E-46 |
| MS.gene060634 | MS.gene70655 | -0.804501 | 2.10E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene70655 | MS.gene24509 | PPI |
| MS.gene005697 | MS.gene70655 | PPI |
| MS.gene70655 | MS.gene074000 | PPI |
| MS.gene70655 | MS.gene25990 | PPI |
| MS.gene70655 | MS.gene40328 | PPI |
| MS.gene70655 | MS.gene21355 | PPI |
| MS.gene70655 | MS.gene24508 | PPI |
| MS.gene051663 | MS.gene70655 | PPI |
| MS.gene70655 | MS.gene00537 | PPI |
| MS.gene70655 | MS.gene00538 | PPI |
| MS.gene70655 | MS.gene031195 | PPI |
| MS.gene70655 | MS.gene21583 | PPI |
| MS.gene70655 | MS.gene051662 | PPI |
| MS.gene70655 | MS.gene21581 | PPI |
| MS.gene006641 | MS.gene70655 | PPI |
| MS.gene70655 | MS.gene031197 | PPI |
| MS.gene70655 | MS.gene29382 | PPI |
| MS.gene70655 | MS.gene039040 | PPI |
| MS.gene70655 | MS.gene75319 | PPI |
| MS.gene70655 | MS.gene045566 | PPI |
| MS.gene66498 | MS.gene70655 | PPI |
| MS.gene70655 | MS.gene75317 | PPI |
| MS.gene70655 | MS.gene66497 | PPI |
| MS.gene70655 | MS.gene92186 | PPI |
| MS.gene70655 | MS.gene21357 | PPI |
| MS.gene022326 | MS.gene70655 | PPI |
| MS.gene014672 | MS.gene70655 | PPI |
| MS.gene70655 | MS.gene005698 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70655.t1 | MTR_3g110030 | 94.845 | 97 | 5 | 0 | 1 | 97 | 1 | 97 | 1.44e-62 | 185 |
| MS.gene70655.t1 | MTR_7g089300 | 74.227 | 97 | 25 | 0 | 1 | 97 | 1 | 97 | 1.30e-48 | 149 |
| MS.gene70655.t1 | MTR_3g077350 | 84.706 | 85 | 13 | 0 | 1 | 85 | 1 | 85 | 2.44e-47 | 146 |
| MS.gene70655.t1 | MTR_3g089960 | 88.095 | 42 | 5 | 0 | 56 | 97 | 212 | 253 | 1.90e-20 | 82.4 |
| MS.gene70655.t1 | MTR_3g077580 | 89.189 | 37 | 4 | 0 | 1 | 37 | 1 | 37 | 7.25e-17 | 69.7 |
| MS.gene70655.t1 | MTR_2g043230 | 40.000 | 95 | 55 | 2 | 3 | 95 | 159 | 253 | 1.89e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70655.t1 | AT1G23100 | 74.227 | 97 | 25 | 0 | 1 | 97 | 1 | 97 | 1.97e-51 | 157 |
| MS.gene70655.t1 | AT1G14980 | 72.165 | 97 | 27 | 0 | 1 | 97 | 1 | 97 | 6.30e-50 | 153 |
| MS.gene70655.t1 | AT1G14980 | 70.732 | 82 | 24 | 0 | 1 | 82 | 1 | 82 | 9.76e-39 | 124 |
Find 37 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGTCATCAAGCTTAATT+TGG | 0.119195 | 3.1:+63588259 | None:intergenic |
| GTTTAGGGATGAGGACATTT+TGG | 0.181417 | 3.1:-63588118 | MS.gene70655:CDS |
| TTTCAGGTTCCATTTGTTTA+GGG | 0.258181 | 3.1:-63588133 | MS.gene70655:intron |
| CCTGGGTTGCGAGACAAATC+TGG | 0.318556 | 3.1:-63588345 | MS.gene70655:CDS |
| AAGGTGGTAGCTGTTGGTCC+TGG | 0.358380 | 3.1:-63588363 | MS.gene70655:CDS |
| GAGAATACCGGCGGAGGTCT+TGG | 0.361115 | 3.1:+63588419 | None:intergenic |
| ATTTGGGAGCCACCATATTC+CGG | 0.363161 | 3.1:+63588276 | None:intergenic |
| TTAAGCTGGGAAGTCTTCTC+TGG | 0.397570 | 3.1:+63588393 | None:intergenic |
| TTTGTCATCAAGCTTAATTT+GGG | 0.416340 | 3.1:+63588260 | None:intergenic |
| AGGTGACCACGTGCTCTTGC+CGG | 0.430535 | 3.1:-63588295 | MS.gene70655:CDS |
| CACGTGCTCTTGCCGGAATA+TGG | 0.430635 | 3.1:-63588288 | MS.gene70655:CDS |
| TGTCCTCATCCCTAAACAAA+TGG | 0.441581 | 3.1:+63588124 | None:intergenic |
| GTTCCATTTGTTTAGGGATG+AGG | 0.449740 | 3.1:-63588127 | MS.gene70655:CDS |
| CCACCTTTGCTGAATTAAGC+TGG | 0.450426 | 3.1:+63588379 | None:intergenic |
| TTCTACGAGGATGCGGTTGA+AGG | 0.455539 | 3.1:+63588458 | None:intergenic |
| ATTCCATTGTCTGTCAAAGA+AGG | 0.468211 | 3.1:-63588315 | MS.gene70655:CDS |
| ACGAGGATGCGGTTGAAGGT+TGG | 0.500516 | 3.1:+63588462 | None:intergenic |
| CTTCTCTGGGAGGAGAATAC+CGG | 0.509700 | 3.1:+63588407 | None:intergenic |
| CACCTTTGCTGAATTAAGCT+GGG | 0.525240 | 3.1:+63588380 | None:intergenic |
| TGGGCATTCTGCATGATTGA+CGG | 0.526635 | 3.1:-63588098 | None:intergenic |
| CCGGCGGAGGTCTTGGAAGG+AGG | 0.534438 | 3.1:+63588426 | None:intergenic |
| AGGTGGTAGCTGTTGGTCCT+GGG | 0.536005 | 3.1:-63588362 | MS.gene70655:CDS |
| CTGGGTTGCGAGACAAATCT+GGG | 0.543620 | 3.1:-63588344 | MS.gene70655:CDS |
| CGGCGGAGGTCTTGGAAGGA+GGG | 0.557534 | 3.1:+63588427 | None:intergenic |
| TCACCTTCTTTGACAGACAA+TGG | 0.564478 | 3.1:+63588312 | None:intergenic |
| CCAGATTTGTCTCGCAACCC+AGG | 0.568286 | 3.1:+63588345 | None:intergenic |
| CCAGCTTAATTCAGCAAAGG+TGG | 0.576003 | 3.1:-63588379 | MS.gene70655:CDS |
| CCTCCTTCCAAGACCTCCGC+CGG | 0.582903 | 3.1:-63588426 | MS.gene70655:CDS |
| TCAGCAAAGGTGGTAGCTGT+TGG | 0.591051 | 3.1:-63588369 | MS.gene70655:CDS |
| TTCCCAGCTTAATTCAGCAA+AGG | 0.592493 | 3.1:-63588382 | MS.gene70655:CDS |
| ATACCGGCGGAGGTCTTGGA+AGG | 0.597082 | 3.1:+63588423 | None:intergenic |
| TAAGCTGGGAAGTCTTCTCT+GGG | 0.609151 | 3.1:+63588394 | None:intergenic |
| GTGCTCTTGCCGGAATATGG+TGG | 0.611791 | 3.1:-63588285 | MS.gene70655:CDS |
| TGGGAGGAGAATACCGGCGG+AGG | 0.622626 | 3.1:+63588413 | None:intergenic |
| CATATTCCGGCAAGAGCACG+TGG | 0.633716 | 3.1:+63588289 | None:intergenic |
| CTCTGGGAGGAGAATACCGG+CGG | 0.652833 | 3.1:+63588410 | None:intergenic |
| GCTGGGAAGTCTTCTCTGGG+AGG | 0.712584 | 3.1:+63588397 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTGTCATCAAGCTTAATTT+GGG | + | chr3.1:63588324-63588343 | None:intergenic | 25.0% |
| !! | ATGACAAAGAGTATGTAATA+TGG | - | chr3.1:63588336-63588355 | MS.gene70655:CDS | 25.0% |
| !! | TGAATGTGTTTCTTTTTATG+TGG | - | chr3.1:63588381-63588400 | MS.gene70655:CDS | 25.0% |
| !!! | TACAAGTTGCTTTATTTTTC+AGG | - | chr3.1:63588432-63588451 | MS.gene70655:CDS | 25.0% |
| CTTTGTCATCAAGCTTAATT+TGG | + | chr3.1:63588325-63588344 | None:intergenic | 30.0% | |
| !! | TTTCAGGTTCCATTTGTTTA+GGG | - | chr3.1:63588448-63588467 | MS.gene70655:CDS | 30.0% |
| !!! | TTTTCAGGTTCCATTTGTTT+AGG | - | chr3.1:63588447-63588466 | MS.gene70655:CDS | 30.0% |
| ATTCCATTGTCTGTCAAAGA+AGG | - | chr3.1:63588266-63588285 | MS.gene70655:CDS | 35.0% | |
| ! | TTTAGGGATGAGGACATTTT+GGG | - | chr3.1:63588464-63588483 | MS.gene70655:CDS | 35.0% |
| !! | TTTTATGTGGACTGCTAACT+TGG | - | chr3.1:63588394-63588413 | MS.gene70655:CDS | 35.0% |
| !!! | CAATTTTTTCTACGAGGATG+CGG | + | chr3.1:63588133-63588152 | None:intergenic | 35.0% |
| CACCTTTGCTGAATTAAGCT+GGG | + | chr3.1:63588204-63588223 | None:intergenic | 40.0% | |
| GTTCCATTTGTTTAGGGATG+AGG | - | chr3.1:63588454-63588473 | MS.gene70655:CDS | 40.0% | |
| TCACCTTCTTTGACAGACAA+TGG | + | chr3.1:63588272-63588291 | None:intergenic | 40.0% | |
| TGTCCTCATCCCTAAACAAA+TGG | + | chr3.1:63588460-63588479 | None:intergenic | 40.0% | |
| TTCCCAGCTTAATTCAGCAA+AGG | - | chr3.1:63588199-63588218 | MS.gene70655:intron | 40.0% | |
| ! | GTTTAGGGATGAGGACATTT+TGG | - | chr3.1:63588463-63588482 | MS.gene70655:CDS | 40.0% |
| !!! | GAGGGACAATTTTTTCTACG+AGG | + | chr3.1:63588139-63588158 | None:intergenic | 40.0% |
| ATTTGGGAGCCACCATATTC+CGG | + | chr3.1:63588308-63588327 | None:intergenic | 45.0% | |
| CCACCTTTGCTGAATTAAGC+TGG | + | chr3.1:63588205-63588224 | None:intergenic | 45.0% | |
| CCAGCTTAATTCAGCAAAGG+TGG | - | chr3.1:63588202-63588221 | MS.gene70655:intron | 45.0% | |
| TAAGCTGGGAAGTCTTCTCT+GGG | + | chr3.1:63588190-63588209 | None:intergenic | 45.0% | |
| TTAAGCTGGGAAGTCTTCTC+TGG | + | chr3.1:63588191-63588210 | None:intergenic | 45.0% | |
| CTGGGTTGCGAGACAAATCT+GGG | - | chr3.1:63588237-63588256 | MS.gene70655:intron | 50.0% | |
| TCAGCAAAGGTGGTAGCTGT+TGG | - | chr3.1:63588212-63588231 | MS.gene70655:intron | 50.0% | |
| TTCTACGAGGATGCGGTTGA+AGG | + | chr3.1:63588126-63588145 | None:intergenic | 50.0% | |
| ! | CTTCTCTGGGAGGAGAATAC+CGG | + | chr3.1:63588177-63588196 | None:intergenic | 50.0% |
| AAGGTGGTAGCTGTTGGTCC+TGG | - | chr3.1:63588218-63588237 | MS.gene70655:intron | 55.0% | |
| ACGAGGATGCGGTTGAAGGT+TGG | + | chr3.1:63588122-63588141 | None:intergenic | 55.0% | |
| AGGTGGTAGCTGTTGGTCCT+GGG | - | chr3.1:63588219-63588238 | MS.gene70655:intron | 55.0% | |
| CACGTGCTCTTGCCGGAATA+TGG | - | chr3.1:63588293-63588312 | MS.gene70655:CDS | 55.0% | |
| CCAGATTTGTCTCGCAACCC+AGG | + | chr3.1:63588239-63588258 | None:intergenic | 55.0% | |
| CCTGGGTTGCGAGACAAATC+TGG | - | chr3.1:63588236-63588255 | MS.gene70655:intron | 55.0% | |
| GTGCTCTTGCCGGAATATGG+TGG | - | chr3.1:63588296-63588315 | MS.gene70655:CDS | 55.0% | |
| ! | CATATTCCGGCAAGAGCACG+TGG | + | chr3.1:63588295-63588314 | None:intergenic | 55.0% |
| AGGTGACCACGTGCTCTTGC+CGG | - | chr3.1:63588286-63588305 | MS.gene70655:CDS | 60.0% | |
| ATACCGGCGGAGGTCTTGGA+AGG | + | chr3.1:63588161-63588180 | None:intergenic | 60.0% | |
| GAGAATACCGGCGGAGGTCT+TGG | + | chr3.1:63588165-63588184 | None:intergenic | 60.0% | |
| GCTGGGAAGTCTTCTCTGGG+AGG | + | chr3.1:63588187-63588206 | None:intergenic | 60.0% | |
| ! | CTCTGGGAGGAGAATACCGG+CGG | + | chr3.1:63588174-63588193 | None:intergenic | 60.0% |
| CCTCCTTCCAAGACCTCCGC+CGG | - | chr3.1:63588155-63588174 | MS.gene70655:intron | 65.0% | |
| CGGCGGAGGTCTTGGAAGGA+GGG | + | chr3.1:63588157-63588176 | None:intergenic | 65.0% | |
| TGGGAGGAGAATACCGGCGG+AGG | + | chr3.1:63588171-63588190 | None:intergenic | 65.0% | |
| CCGGCGGAGGTCTTGGAAGG+AGG | + | chr3.1:63588158-63588177 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 63588101 | 63588502 | 63588101 | ID=MS.gene70655 |
| chr3.1 | mRNA | 63588101 | 63588502 | 63588101 | ID=MS.gene70655.t1;Parent=MS.gene70655 |
| chr3.1 | exon | 63588258 | 63588502 | 63588258 | ID=MS.gene70655.t1.exon1;Parent=MS.gene70655.t1 |
| chr3.1 | CDS | 63588258 | 63588502 | 63588258 | ID=cds.MS.gene70655.t1;Parent=MS.gene70655.t1 |
| chr3.1 | exon | 63588101 | 63588149 | 63588101 | ID=MS.gene70655.t1.exon2;Parent=MS.gene70655.t1 |
| chr3.1 | CDS | 63588101 | 63588149 | 63588101 | ID=cds.MS.gene70655.t1;Parent=MS.gene70655.t1 |
| Gene Sequence |
| Protein sequence |
