Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031195.t1 | XP_013461973.1 | 100 | 97 | 0 | 0 | 1 | 97 | 1 | 97 | 8.60E-46 | 192.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031195.t1 | P34893 | 75.3 | 97 | 24 | 0 | 1 | 97 | 1 | 97 | 3.7e-37 | 155.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031195.t1 | G7ZWL8 | 100.0 | 97 | 0 | 0 | 1 | 97 | 1 | 97 | 6.2e-46 | 192.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene66498 | MS.gene031195 | PPI |
| MS.gene031195 | MS.gene75317 | PPI |
| MS.gene031195 | MS.gene66497 | PPI |
| MS.gene031195 | MS.gene92186 | PPI |
| MS.gene031195 | MS.gene21357 | PPI |
| MS.gene031195 | MS.gene24509 | PPI |
| MS.gene70655 | MS.gene031195 | PPI |
| MS.gene031195 | MS.gene074000 | PPI |
| MS.gene031195 | MS.gene21355 | PPI |
| MS.gene031195 | MS.gene005698 | PPI |
| MS.gene022326 | MS.gene031195 | PPI |
| MS.gene014672 | MS.gene031195 | PPI |
| MS.gene031195 | MS.gene24508 | PPI |
| MS.gene031195 | MS.gene00537 | PPI |
| MS.gene031195 | MS.gene00538 | PPI |
| MS.gene051663 | MS.gene031195 | PPI |
| MS.gene031195 | MS.gene21583 | PPI |
| MS.gene031195 | MS.gene25990 | PPI |
| MS.gene031195 | MS.gene051662 | PPI |
| MS.gene031195 | MS.gene21581 | PPI |
| MS.gene005697 | MS.gene031195 | PPI |
| MS.gene031195 | MS.gene40328 | PPI |
| MS.gene006641 | MS.gene031195 | PPI |
| MS.gene031195 | MS.gene031197 | PPI |
| MS.gene031195 | MS.gene29382 | PPI |
| MS.gene031195 | MS.gene039040 | PPI |
| MS.gene031195 | MS.gene75319 | PPI |
| MS.gene031195 | MS.gene045566 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031195.t1 | MTR_3g110030 | 100.000 | 97 | 0 | 0 | 1 | 97 | 1 | 97 | 9.29e-66 | 193 |
| MS.gene031195.t1 | MTR_3g077350 | 84.706 | 85 | 13 | 0 | 1 | 85 | 1 | 85 | 2.00e-48 | 149 |
| MS.gene031195.t1 | MTR_7g089300 | 74.227 | 97 | 25 | 0 | 1 | 97 | 1 | 97 | 3.13e-48 | 149 |
| MS.gene031195.t1 | MTR_3g089960 | 85.714 | 42 | 6 | 0 | 56 | 97 | 212 | 253 | 2.84e-19 | 79.3 |
| MS.gene031195.t1 | MTR_3g077580 | 86.486 | 37 | 5 | 0 | 1 | 37 | 1 | 37 | 1.21e-16 | 68.9 |
| MS.gene031195.t1 | MTR_2g043230 | 38.947 | 95 | 56 | 2 | 3 | 95 | 159 | 253 | 1.45e-11 | 58.5 |
| MS.gene031195.t1 | MTR_4g124910 | 37.778 | 90 | 54 | 2 | 7 | 95 | 64 | 152 | 3.05e-11 | 57.8 |
| MS.gene031195.t1 | MTR_4g124910 | 37.895 | 95 | 57 | 2 | 3 | 95 | 158 | 252 | 8.06e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031195.t1 | AT1G23100 | 75.258 | 97 | 24 | 0 | 1 | 97 | 1 | 97 | 7.18e-52 | 158 |
| MS.gene031195.t1 | AT1G14980 | 75.258 | 97 | 24 | 0 | 1 | 97 | 1 | 97 | 5.22e-51 | 155 |
| MS.gene031195.t1 | AT1G14980 | 75.610 | 82 | 20 | 0 | 1 | 82 | 1 | 82 | 5.04e-41 | 130 |
| MS.gene031195.t1 | AT5G20720 | 36.842 | 95 | 58 | 2 | 3 | 95 | 158 | 252 | 3.53e-11 | 57.8 |
| MS.gene031195.t1 | AT5G20720 | 36.842 | 95 | 58 | 2 | 3 | 95 | 158 | 252 | 3.53e-11 | 57.8 |
| MS.gene031195.t1 | AT5G20720 | 36.842 | 95 | 58 | 2 | 3 | 95 | 158 | 252 | 3.53e-11 | 57.8 |
| MS.gene031195.t1 | AT5G20720 | 36.842 | 95 | 58 | 2 | 3 | 95 | 158 | 252 | 3.53e-11 | 57.8 |
Find 33 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTAGGGATGAGGACATTT+TGG | 0.181417 | 3.1:+87521692 | MS.gene031195:CDS |
| ACAAAGAGTTTCATTTGTTT+AGG | 0.264498 | 3.1:+87521676 | MS.gene031195:CDS |
| GTTTGAAAACAGCTTAATTC+AGG | 0.277309 | 3.1:+87521531 | MS.gene031195:intron |
| CAAAGAGTTTCATTTGTTTA+GGG | 0.318510 | 3.1:+87521677 | MS.gene031195:CDS |
| CACGTTCTCTTGCCGGAATA+TGG | 0.350941 | 3.1:+87521630 | MS.gene031195:CDS |
| CTCGACGAGGATGCGGTTGA+AGG | 0.354705 | 3.1:-87521370 | None:intergenic |
| CAAACCTGGGAGGTCTTCTC+AGG | 0.357034 | 3.1:-87521435 | None:intergenic |
| AAGAATACCGGCGGAGGTCT+TGG | 0.361115 | 3.1:-87521409 | None:intergenic |
| ATTTGAGAGCCACCATATTC+CGG | 0.366812 | 3.1:-87521642 | None:intergenic |
| CAAATCTGGGAATTTGATTC+CGG | 0.387419 | 3.1:+87521587 | MS.gene031195:CDS |
| CCTGGATCGAGAGACAAATC+TGG | 0.418950 | 3.1:+87521573 | MS.gene031195:CDS |
| AGGTGACCACGTTCTCTTGC+CGG | 0.420998 | 3.1:+87521623 | MS.gene031195:CDS |
| GTTTCATTTGTTTAGGGATG+AGG | 0.443213 | 3.1:+87521683 | MS.gene031195:CDS |
| ACGAGGATGCGGTTGAAGGT+TGG | 0.459998 | 3.1:-87521366 | None:intergenic |
| CCAGATTTGTCTCTCGATCC+AGG | 0.466866 | 3.1:-87521573 | None:intergenic |
| TCAGGAAAGGTTGTAGCTGT+TGG | 0.477328 | 3.1:+87521549 | MS.gene031195:CDS |
| TCTCCCTGAGAAGACCTCCC+AGG | 0.483550 | 3.1:+87521431 | MS.gene031195:CDS |
| ATTCCGGTGTCTGTCAAAGA+AGG | 0.501127 | 3.1:+87521603 | MS.gene031195:CDS |
| CCGGCGGAGGTCTTGGAAGG+AGG | 0.529929 | 3.1:-87521402 | None:intergenic |
| AAAACAGCTTAATTCAGGAA+AGG | 0.534414 | 3.1:+87521536 | MS.gene031195:intron |
| CGGCGGAGGTCTTGGAAGGA+GGG | 0.543498 | 3.1:-87521401 | None:intergenic |
| AAGGTTGTAGCTGTTGGACC+TGG | 0.547728 | 3.1:+87521555 | MS.gene031195:CDS |
| CTTCTCAGGGAGAAGAATAC+CGG | 0.548614 | 3.1:-87521421 | None:intergenic |
| CTGGATCGAGAGACAAATCT+GGG | 0.579563 | 3.1:+87521574 | MS.gene031195:CDS |
| CCTCCTTCCAAGACCTCCGC+CGG | 0.595748 | 3.1:+87521402 | MS.gene031195:CDS |
| ATACCGGCGGAGGTCTTGGA+AGG | 0.597082 | 3.1:-87521405 | None:intergenic |
| GTTCTCTTGCCGGAATATGG+TGG | 0.611791 | 3.1:+87521633 | MS.gene031195:CDS |
| TCACCTTCTTTGACAGACAC+CGG | 0.618503 | 3.1:-87521606 | None:intergenic |
| AGGGAGAAGAATACCGGCGG+AGG | 0.620138 | 3.1:-87521415 | None:intergenic |
| CATATTCCGGCAAGAGAACG+TGG | 0.633016 | 3.1:-87521629 | None:intergenic |
| AAACCTGGGAGGTCTTCTCA+GGG | 0.670497 | 3.1:-87521434 | None:intergenic |
| CTCAGGGAGAAGAATACCGG+CGG | 0.675295 | 3.1:-87521418 | None:intergenic |
| AGAGTTTCTACAAACCTGGG+AGG | 0.716301 | 3.1:-87521445 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAAGAGTTTCATTTGTTT+AGG | + | chr3.1:87521676-87521695 | MS.gene031195:CDS | 25.0% |
| ! | CAAAGAGTTTCATTTGTTTA+GGG | + | chr3.1:87521677-87521696 | MS.gene031195:CDS | 25.0% |
| !! | GATTTGGATTTAATTGATTC+TGG | + | chr3.1:87521509-87521528 | MS.gene031195:intron | 25.0% |
| AAAACAGCTTAATTCAGGAA+AGG | + | chr3.1:87521536-87521555 | MS.gene031195:intron | 30.0% | |
| GTTTGAAAACAGCTTAATTC+AGG | + | chr3.1:87521531-87521550 | MS.gene031195:intron | 30.0% | |
| AAGAGAGTTTCTACAAACCT+GGG | - | chr3.1:87521451-87521470 | None:intergenic | 35.0% | |
| CAAATCTGGGAATTTGATTC+CGG | + | chr3.1:87521587-87521606 | MS.gene031195:CDS | 35.0% | |
| GTTTCATTTGTTTAGGGATG+AGG | + | chr3.1:87521683-87521702 | MS.gene031195:CDS | 35.0% | |
| TGTCTCTGTGTTAATCGATT+TGG | + | chr3.1:87521493-87521512 | MS.gene031195:intron | 35.0% | |
| ! | TTTAGGGATGAGGACATTTT+GGG | + | chr3.1:87521693-87521712 | MS.gene031195:CDS | 35.0% |
| ATTTGAGAGCCACCATATTC+CGG | - | chr3.1:87521645-87521664 | None:intergenic | 40.0% | |
| GAAGAGAGTTTCTACAAACC+TGG | - | chr3.1:87521452-87521471 | None:intergenic | 40.0% | |
| ! | GTTTAGGGATGAGGACATTT+TGG | + | chr3.1:87521692-87521711 | MS.gene031195:CDS | 40.0% |
| AGAGTTTCTACAAACCTGGG+AGG | - | chr3.1:87521448-87521467 | None:intergenic | 45.0% | |
| ATTCCGGTGTCTGTCAAAGA+AGG | + | chr3.1:87521603-87521622 | MS.gene031195:CDS | 45.0% | |
| CTGGATCGAGAGACAAATCT+GGG | + | chr3.1:87521574-87521593 | MS.gene031195:CDS | 45.0% | |
| CTTCTCAGGGAGAAGAATAC+CGG | - | chr3.1:87521424-87521443 | None:intergenic | 45.0% | |
| TCACCTTCTTTGACAGACAC+CGG | - | chr3.1:87521609-87521628 | None:intergenic | 45.0% | |
| TCAGGAAAGGTTGTAGCTGT+TGG | + | chr3.1:87521549-87521568 | MS.gene031195:CDS | 45.0% | |
| ! | TGATTTTCTCGACGAGGATG+CGG | - | chr3.1:87521380-87521399 | None:intergenic | 45.0% |
| AAACCTGGGAGGTCTTCTCA+GGG | - | chr3.1:87521437-87521456 | None:intergenic | 50.0% | |
| AAGGTTGTAGCTGTTGGACC+TGG | + | chr3.1:87521555-87521574 | MS.gene031195:CDS | 50.0% | |
| CACGTTCTCTTGCCGGAATA+TGG | + | chr3.1:87521630-87521649 | MS.gene031195:CDS | 50.0% | |
| CATATTCCGGCAAGAGAACG+TGG | - | chr3.1:87521632-87521651 | None:intergenic | 50.0% | |
| CCAGATTTGTCTCTCGATCC+AGG | - | chr3.1:87521576-87521595 | None:intergenic | 50.0% | |
| CCTGGATCGAGAGACAAATC+TGG | + | chr3.1:87521573-87521592 | MS.gene031195:CDS | 50.0% | |
| GTTCTCTTGCCGGAATATGG+TGG | + | chr3.1:87521633-87521652 | MS.gene031195:CDS | 50.0% | |
| ! | GAGGGATGATTTTCTCGACG+AGG | - | chr3.1:87521386-87521405 | None:intergenic | 50.0% |
| AAGAATACCGGCGGAGGTCT+TGG | - | chr3.1:87521412-87521431 | None:intergenic | 55.0% | |
| ACGAGGATGCGGTTGAAGGT+TGG | - | chr3.1:87521369-87521388 | None:intergenic | 55.0% | |
| AGGTGACCACGTTCTCTTGC+CGG | + | chr3.1:87521623-87521642 | MS.gene031195:CDS | 55.0% | |
| CAAACCTGGGAGGTCTTCTC+AGG | - | chr3.1:87521438-87521457 | None:intergenic | 55.0% | |
| CTCAGGGAGAAGAATACCGG+CGG | - | chr3.1:87521421-87521440 | None:intergenic | 55.0% | |
| AGGGAGAAGAATACCGGCGG+AGG | - | chr3.1:87521418-87521437 | None:intergenic | 60.0% | |
| ATACCGGCGGAGGTCTTGGA+AGG | - | chr3.1:87521408-87521427 | None:intergenic | 60.0% | |
| CTCGACGAGGATGCGGTTGA+AGG | - | chr3.1:87521373-87521392 | None:intergenic | 60.0% | |
| TCTCCCTGAGAAGACCTCCC+AGG | + | chr3.1:87521431-87521450 | MS.gene031195:CDS | 60.0% | |
| CCTCCTTCCAAGACCTCCGC+CGG | + | chr3.1:87521402-87521421 | MS.gene031195:CDS | 65.0% | |
| CGGCGGAGGTCTTGGAAGGA+GGG | - | chr3.1:87521404-87521423 | None:intergenic | 65.0% | |
| CCGGCGGAGGTCTTGGAAGG+AGG | - | chr3.1:87521405-87521424 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 87521348 | 87521731 | 87521348 | ID=MS.gene031195 |
| chr3.1 | mRNA | 87521348 | 87521731 | 87521348 | ID=MS.gene031195.t1;Parent=MS.gene031195 |
| chr3.1 | exon | 87521348 | 87521452 | 87521348 | ID=MS.gene031195.t1.exon1;Parent=MS.gene031195.t1 |
| chr3.1 | CDS | 87521348 | 87521452 | 87521348 | ID=cds.MS.gene031195.t1;Parent=MS.gene031195.t1 |
| chr3.1 | exon | 87521543 | 87521731 | 87521543 | ID=MS.gene031195.t1.exon2;Parent=MS.gene031195.t1 |
| chr3.1 | CDS | 87521543 | 87521731 | 87521543 | ID=cds.MS.gene031195.t1;Parent=MS.gene031195.t1 |
| Gene Sequence |
| Protein sequence |
