Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29382.t1 | XP_004505282.1 | 96.9 | 97 | 3 | 0 | 1 | 97 | 1 | 97 | 2.10E-44 | 188 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29382.t1 | P34893 | 72.2 | 97 | 27 | 0 | 1 | 97 | 1 | 97 | 3.1e-36 | 152.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29382.t1 | A0A1S2YIG4 | 96.9 | 97 | 3 | 0 | 1 | 97 | 1 | 97 | 1.5e-44 | 188.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene29382 | MS.gene074000 | PPI |
| MS.gene006641 | MS.gene29382 | PPI |
| MS.gene29382 | MS.gene21355 | PPI |
| MS.gene29382 | MS.gene005698 | PPI |
| MS.gene29382 | MS.gene24508 | PPI |
| MS.gene29382 | MS.gene00537 | PPI |
| MS.gene29382 | MS.gene00538 | PPI |
| MS.gene29382 | MS.gene21583 | PPI |
| MS.gene29382 | MS.gene25990 | PPI |
| MS.gene66498 | MS.gene29382 | PPI |
| MS.gene29382 | MS.gene051662 | PPI |
| MS.gene29382 | MS.gene21581 | PPI |
| MS.gene29382 | MS.gene40328 | PPI |
| MS.gene29382 | MS.gene031197 | PPI |
| MS.gene29382 | MS.gene039040 | PPI |
| MS.gene29382 | MS.gene75319 | PPI |
| MS.gene29382 | MS.gene045566 | PPI |
| MS.gene70655 | MS.gene29382 | PPI |
| MS.gene29382 | MS.gene75317 | PPI |
| MS.gene29382 | MS.gene66497 | PPI |
| MS.gene29382 | MS.gene92186 | PPI |
| MS.gene022326 | MS.gene29382 | PPI |
| MS.gene014672 | MS.gene29382 | PPI |
| MS.gene21357 | MS.gene29382 | PPI |
| MS.gene051663 | MS.gene29382 | PPI |
| MS.gene031195 | MS.gene29382 | PPI |
| MS.gene29382 | MS.gene24509 | PPI |
| MS.gene005697 | MS.gene29382 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29382.t1 | MTR_3g110030 | 94.845 | 97 | 5 | 0 | 1 | 97 | 1 | 97 | 1.44e-62 | 185 |
| MS.gene29382.t1 | MTR_7g089300 | 74.227 | 97 | 25 | 0 | 1 | 97 | 1 | 97 | 1.30e-48 | 149 |
| MS.gene29382.t1 | MTR_3g077350 | 84.706 | 85 | 13 | 0 | 1 | 85 | 1 | 85 | 2.44e-47 | 146 |
| MS.gene29382.t1 | MTR_3g089960 | 88.095 | 42 | 5 | 0 | 56 | 97 | 212 | 253 | 1.90e-20 | 82.4 |
| MS.gene29382.t1 | MTR_3g077580 | 89.189 | 37 | 4 | 0 | 1 | 37 | 1 | 37 | 7.25e-17 | 69.7 |
| MS.gene29382.t1 | MTR_2g043230 | 40.000 | 95 | 55 | 2 | 3 | 95 | 159 | 253 | 1.89e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29382.t1 | AT1G23100 | 74.227 | 97 | 25 | 0 | 1 | 97 | 1 | 97 | 1.97e-51 | 157 |
| MS.gene29382.t1 | AT1G14980 | 72.165 | 97 | 27 | 0 | 1 | 97 | 1 | 97 | 6.30e-50 | 153 |
| MS.gene29382.t1 | AT1G14980 | 70.732 | 82 | 24 | 0 | 1 | 82 | 1 | 82 | 9.76e-39 | 124 |
Find 38 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGTCATCAAGCTTAATT+TGG | 0.119195 | 3.2:+68434948 | None:intergenic |
| GTTTAGGGATGAGGACATTT+TGG | 0.181417 | 3.2:-68434807 | MS.gene29382:CDS |
| TTTCAGGTTCCATTTGTTTA+GGG | 0.258181 | 3.2:-68434822 | MS.gene29382:intron |
| CCTGGGTTGCGAGACAAATC+TGG | 0.318556 | 3.2:-68435034 | MS.gene29382:CDS |
| AAGGTGGTAGCTGTTGGTCC+TGG | 0.358380 | 3.2:-68435052 | MS.gene29382:CDS |
| GAGAATACCGGCGGAGGTCT+TGG | 0.361115 | 3.2:+68435108 | None:intergenic |
| ATTTGGGAGCCACCATATTC+CGG | 0.363161 | 3.2:+68434965 | None:intergenic |
| TTAAGCTGGGAAGTCTTCTC+TGG | 0.397570 | 3.2:+68435082 | None:intergenic |
| TTTGTCATCAAGCTTAATTT+GGG | 0.416340 | 3.2:+68434949 | None:intergenic |
| CACGTGCTCTTGCCGGAATA+TGG | 0.430635 | 3.2:-68434977 | MS.gene29382:CDS |
| TGTCCTCATCCCTAAACAAA+TGG | 0.441581 | 3.2:+68434813 | None:intergenic |
| AGGTGACCACGTGCTCTTGC+CGG | 0.446397 | 3.2:-68434984 | MS.gene29382:CDS |
| GTTCCATTTGTTTAGGGATG+AGG | 0.449740 | 3.2:-68434816 | MS.gene29382:CDS |
| CCACCTTTGCTGAATTAAGC+TGG | 0.450426 | 3.2:+68435068 | None:intergenic |
| TTCTACGAGGATGCGGTTGA+AGG | 0.455539 | 3.2:+68435147 | None:intergenic |
| ACGAGGATGCGGTTGAAGGT+TGG | 0.500516 | 3.2:+68435151 | None:intergenic |
| CTTCTCTGGGAGGAGAATAC+CGG | 0.509700 | 3.2:+68435096 | None:intergenic |
| CACCTTTGCTGAATTAAGCT+GGG | 0.525240 | 3.2:+68435069 | None:intergenic |
| TGGGCATTCTGCATGATTGA+CGG | 0.526635 | 3.2:-68434787 | None:intergenic |
| CCGGCGGAGGTCTTGGAAGG+AGG | 0.534438 | 3.2:+68435115 | None:intergenic |
| AGGTGGTAGCTGTTGGTCCT+GGG | 0.536005 | 3.2:-68435051 | MS.gene29382:CDS |
| CTGGGTTGCGAGACAAATCT+GGG | 0.543620 | 3.2:-68435033 | MS.gene29382:CDS |
| TTTGATTCCATTGTCTGTCA+AGG | 0.556707 | 3.2:-68435008 | MS.gene29382:CDS |
| CGGCGGAGGTCTTGGAAGGA+GGG | 0.557534 | 3.2:+68435116 | None:intergenic |
| ATTCCATTGTCTGTCAAGGA+AGG | 0.559362 | 3.2:-68435004 | MS.gene29382:CDS |
| CCAGATTTGTCTCGCAACCC+AGG | 0.568286 | 3.2:+68435034 | None:intergenic |
| CCAGCTTAATTCAGCAAAGG+TGG | 0.576003 | 3.2:-68435068 | MS.gene29382:CDS |
| TCACCTTCCTTGACAGACAA+TGG | 0.578277 | 3.2:+68435001 | None:intergenic |
| CCTCCTTCCAAGACCTCCGC+CGG | 0.582903 | 3.2:-68435115 | MS.gene29382:CDS |
| TCAGCAAAGGTGGTAGCTGT+TGG | 0.591051 | 3.2:-68435058 | MS.gene29382:CDS |
| TTCCCAGCTTAATTCAGCAA+AGG | 0.592493 | 3.2:-68435071 | MS.gene29382:CDS |
| ATACCGGCGGAGGTCTTGGA+AGG | 0.597082 | 3.2:+68435112 | None:intergenic |
| TAAGCTGGGAAGTCTTCTCT+GGG | 0.609151 | 3.2:+68435083 | None:intergenic |
| GTGCTCTTGCCGGAATATGG+TGG | 0.611791 | 3.2:-68434974 | MS.gene29382:CDS |
| TGGGAGGAGAATACCGGCGG+AGG | 0.622626 | 3.2:+68435102 | None:intergenic |
| CATATTCCGGCAAGAGCACG+TGG | 0.633716 | 3.2:+68434978 | None:intergenic |
| CTCTGGGAGGAGAATACCGG+CGG | 0.652833 | 3.2:+68435099 | None:intergenic |
| GCTGGGAAGTCTTCTCTGGG+AGG | 0.712584 | 3.2:+68435086 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTGTCATCAAGCTTAATTT+GGG | + | chr3.2:68435013-68435032 | None:intergenic | 25.0% |
| !! | ATGACAAAGAGTATGTAATA+TGG | - | chr3.2:68435025-68435044 | MS.gene29382:CDS | 25.0% |
| !! | TGAATGTGTTTCTTTTTATG+TGG | - | chr3.2:68435070-68435089 | MS.gene29382:CDS | 25.0% |
| !!! | TACAAGTTGCTTTATTTTTC+AGG | - | chr3.2:68435121-68435140 | MS.gene29382:CDS | 25.0% |
| CTTTGTCATCAAGCTTAATT+TGG | + | chr3.2:68435014-68435033 | None:intergenic | 30.0% | |
| ! | TTTTATGTGGAATGCTAACT+TGG | - | chr3.2:68435083-68435102 | MS.gene29382:CDS | 30.0% |
| !! | TTTCAGGTTCCATTTGTTTA+GGG | - | chr3.2:68435137-68435156 | MS.gene29382:CDS | 30.0% |
| !!! | TTTTCAGGTTCCATTTGTTT+AGG | - | chr3.2:68435136-68435155 | MS.gene29382:CDS | 30.0% |
| TTTGATTCCATTGTCTGTCA+AGG | - | chr3.2:68434951-68434970 | MS.gene29382:CDS | 35.0% | |
| ! | TTTAGGGATGAGGACATTTT+GGG | - | chr3.2:68435153-68435172 | MS.gene29382:CDS | 35.0% |
| !!! | CAATTTTTTCTACGAGGATG+CGG | + | chr3.2:68434822-68434841 | None:intergenic | 35.0% |
| ATTCCATTGTCTGTCAAGGA+AGG | - | chr3.2:68434955-68434974 | MS.gene29382:CDS | 40.0% | |
| CACCTTTGCTGAATTAAGCT+GGG | + | chr3.2:68434893-68434912 | None:intergenic | 40.0% | |
| GTTCCATTTGTTTAGGGATG+AGG | - | chr3.2:68435143-68435162 | MS.gene29382:CDS | 40.0% | |
| TGTCCTCATCCCTAAACAAA+TGG | + | chr3.2:68435149-68435168 | None:intergenic | 40.0% | |
| TTCCCAGCTTAATTCAGCAA+AGG | - | chr3.2:68434888-68434907 | MS.gene29382:intron | 40.0% | |
| ! | GTTTAGGGATGAGGACATTT+TGG | - | chr3.2:68435152-68435171 | MS.gene29382:CDS | 40.0% |
| !!! | GAGGGACAATTTTTTCTACG+AGG | + | chr3.2:68434828-68434847 | None:intergenic | 40.0% |
| ATTTGGGAGCCACCATATTC+CGG | + | chr3.2:68434997-68435016 | None:intergenic | 45.0% | |
| CCACCTTTGCTGAATTAAGC+TGG | + | chr3.2:68434894-68434913 | None:intergenic | 45.0% | |
| CCAGCTTAATTCAGCAAAGG+TGG | - | chr3.2:68434891-68434910 | MS.gene29382:intron | 45.0% | |
| TAAGCTGGGAAGTCTTCTCT+GGG | + | chr3.2:68434879-68434898 | None:intergenic | 45.0% | |
| TCACCTTCCTTGACAGACAA+TGG | + | chr3.2:68434961-68434980 | None:intergenic | 45.0% | |
| TTAAGCTGGGAAGTCTTCTC+TGG | + | chr3.2:68434880-68434899 | None:intergenic | 45.0% | |
| CTGGGTTGCGAGACAAATCT+GGG | - | chr3.2:68434926-68434945 | MS.gene29382:intron | 50.0% | |
| TCAGCAAAGGTGGTAGCTGT+TGG | - | chr3.2:68434901-68434920 | MS.gene29382:intron | 50.0% | |
| TTCTACGAGGATGCGGTTGA+AGG | + | chr3.2:68434815-68434834 | None:intergenic | 50.0% | |
| ! | CTTCTCTGGGAGGAGAATAC+CGG | + | chr3.2:68434866-68434885 | None:intergenic | 50.0% |
| AAGGTGGTAGCTGTTGGTCC+TGG | - | chr3.2:68434907-68434926 | MS.gene29382:intron | 55.0% | |
| ACGAGGATGCGGTTGAAGGT+TGG | + | chr3.2:68434811-68434830 | None:intergenic | 55.0% | |
| AGGTGGTAGCTGTTGGTCCT+GGG | - | chr3.2:68434908-68434927 | MS.gene29382:intron | 55.0% | |
| CACGTGCTCTTGCCGGAATA+TGG | - | chr3.2:68434982-68435001 | MS.gene29382:CDS | 55.0% | |
| CCAGATTTGTCTCGCAACCC+AGG | + | chr3.2:68434928-68434947 | None:intergenic | 55.0% | |
| CCTGGGTTGCGAGACAAATC+TGG | - | chr3.2:68434925-68434944 | MS.gene29382:intron | 55.0% | |
| GTGCTCTTGCCGGAATATGG+TGG | - | chr3.2:68434985-68435004 | MS.gene29382:CDS | 55.0% | |
| ! | CATATTCCGGCAAGAGCACG+TGG | + | chr3.2:68434984-68435003 | None:intergenic | 55.0% |
| AGGTGACCACGTGCTCTTGC+CGG | - | chr3.2:68434975-68434994 | MS.gene29382:CDS | 60.0% | |
| ATACCGGCGGAGGTCTTGGA+AGG | + | chr3.2:68434850-68434869 | None:intergenic | 60.0% | |
| GAGAATACCGGCGGAGGTCT+TGG | + | chr3.2:68434854-68434873 | None:intergenic | 60.0% | |
| GCTGGGAAGTCTTCTCTGGG+AGG | + | chr3.2:68434876-68434895 | None:intergenic | 60.0% | |
| ! | CTCTGGGAGGAGAATACCGG+CGG | + | chr3.2:68434863-68434882 | None:intergenic | 60.0% |
| CCTCCTTCCAAGACCTCCGC+CGG | - | chr3.2:68434844-68434863 | MS.gene29382:intron | 65.0% | |
| CGGCGGAGGTCTTGGAAGGA+GGG | + | chr3.2:68434846-68434865 | None:intergenic | 65.0% | |
| TGGGAGGAGAATACCGGCGG+AGG | + | chr3.2:68434860-68434879 | None:intergenic | 65.0% | |
| CCGGCGGAGGTCTTGGAAGG+AGG | + | chr3.2:68434847-68434866 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 68434790 | 68435191 | 68434790 | ID=MS.gene29382 |
| chr3.2 | mRNA | 68434790 | 68435191 | 68434790 | ID=MS.gene29382.t1;Parent=MS.gene29382 |
| chr3.2 | exon | 68434947 | 68435191 | 68434947 | ID=MS.gene29382.t1.exon1;Parent=MS.gene29382.t1 |
| chr3.2 | CDS | 68434947 | 68435191 | 68434947 | ID=cds.MS.gene29382.t1;Parent=MS.gene29382.t1 |
| chr3.2 | exon | 68434790 | 68434838 | 68434790 | ID=MS.gene29382.t1.exon2;Parent=MS.gene29382.t1 |
| chr3.2 | CDS | 68434790 | 68434838 | 68434790 | ID=cds.MS.gene29382.t1;Parent=MS.gene29382.t1 |
| Gene Sequence |
| Protein sequence |
