AlfalfaGEDB | MS.gene031197

M. sativa cultivar XinJiangDaYe / MS.gene031197

KEGG: K04078
GO: GO:0006457 GO:0008150 GO:0009987
PFAM: PF00166.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031197.t1	XP_013461973.1	100	97	0	0	1	97	1	97	8.60E-46	192.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031197.t1	P34893	75.3	97	24	0	1	97	1	97	3.7e-37	155.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031197.t1	G7ZWL8	100.0	97	0	0	1	97	1	97	6.2e-46	192.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene24509	MS.gene031197	PPI
MS.gene031197	MS.gene75317	PPI
MS.gene21357	MS.gene031197	PPI
MS.gene051663	MS.gene031197	PPI
MS.gene031197	MS.gene66497	PPI
MS.gene006641	MS.gene031197	PPI
MS.gene75317	MS.gene031197	PPI
MS.gene045566	MS.gene031197	PPI
MS.gene92186	MS.gene031197	PPI
MS.gene24508	MS.gene031197	PPI
MS.gene005698	MS.gene031197	PPI
MS.gene21583	MS.gene031197	PPI
MS.gene29382	MS.gene031197	PPI
MS.gene66498	MS.gene031197	PPI
MS.gene074000	MS.gene031197	PPI
MS.gene70655	MS.gene031197	PPI
MS.gene031197	MS.gene00537	PPI
MS.gene031197	MS.gene00538	PPI
MS.gene40328	MS.gene031197	PPI
MS.gene031197	MS.gene25990	PPI
MS.gene031197	MS.gene21581	PPI
MS.gene022326	MS.gene031197	PPI
MS.gene014672	MS.gene031197	PPI
MS.gene031197	MS.gene40328	PPI
MS.gene051662	MS.gene031197	PPI
MS.gene031195	MS.gene031197	PPI
MS.gene031197	MS.gene039040	PPI
MS.gene21355	MS.gene031197	PPI
MS.gene005697	MS.gene031197	PPI
MS.gene031197	MS.gene75319	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031197.t1	MTR_3g110030	100.000	97	0	0	1	97	1	97	9.29e-66	193
MS.gene031197.t1	MTR_3g077350	84.706	85	13	0	1	85	1	85	2.00e-48	149
MS.gene031197.t1	MTR_7g089300	74.227	97	25	0	1	97	1	97	3.13e-48	149
MS.gene031197.t1	MTR_3g089960	85.714	42	6	0	56	97	212	253	2.84e-19	79.3
MS.gene031197.t1	MTR_3g077580	86.486	37	5	0	1	37	1	37	1.21e-16	68.9
MS.gene031197.t1	MTR_2g043230	38.947	95	56	2	3	95	159	253	1.45e-11	58.5
MS.gene031197.t1	MTR_4g124910	37.778	90	54	2	7	95	64	152	3.05e-11	57.8
MS.gene031197.t1	MTR_4g124910	37.895	95	57	2	3	95	158	252	8.06e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031197.t1	AT1G23100	75.258	97	24	0	1	97	1	97	7.18e-52	158
MS.gene031197.t1	AT1G14980	75.258	97	24	0	1	97	1	97	5.22e-51	155
MS.gene031197.t1	AT1G14980	75.610	82	20	0	1	82	1	82	5.04e-41	130
MS.gene031197.t1	AT5G20720	36.842	95	58	2	3	95	158	252	3.53e-11	57.8
MS.gene031197.t1	AT5G20720	36.842	95	58	2	3	95	158	252	3.53e-11	57.8
MS.gene031197.t1	AT5G20720	36.842	95	58	2	3	95	158	252	3.53e-11	57.8
MS.gene031197.t1	AT5G20720	36.842	95	58	2	3	95	158	252	3.53e-11	57.8

Find 0 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACAAAGAGTTTCATTTGTTT+AGG	+	21207:12480-12499	MS.gene031197:CDS	25.0%
!	CAAAGAGTTTCATTTGTTTA+GGG	+	21207:12481-12500	MS.gene031197:CDS	25.0%
!!	GATTTGGATTTAATTGATTC+TGG	+	21207:12313-12332	MS.gene031197:intron	25.0%
	AAAACAGCTTAATTCAGGAA+AGG	+	21207:12340-12359	MS.gene031197:intron	30.0%
	GTTTGAAAACAGCTTAATTC+AGG	+	21207:12335-12354	MS.gene031197:intron	30.0%
	AAGAGAGTTTCTACAAACCT+GGG	-	21207:12255-12274	None:intergenic	35.0%
	CAAATCTGGGAATTTGATTC+CGG	+	21207:12391-12410	MS.gene031197:CDS	35.0%
	GTTTCATTTGTTTAGGGATG+AGG	+	21207:12487-12506	MS.gene031197:CDS	35.0%
	TGTCTCTGTGTTAATCGATT+TGG	+	21207:12297-12316	MS.gene031197:intron	35.0%
!	TTTAGGGATGAGGACATTTT+GGG	+	21207:12497-12516	MS.gene031197:CDS	35.0%
	ATTTGAGAGCCACCATATTC+CGG	-	21207:12449-12468	None:intergenic	40.0%
	GAAGAGAGTTTCTACAAACC+TGG	-	21207:12256-12275	None:intergenic	40.0%
!	GTTTAGGGATGAGGACATTT+TGG	+	21207:12496-12515	MS.gene031197:CDS	40.0%
	AGAGTTTCTACAAACCTGGG+AGG	-	21207:12252-12271	None:intergenic	45.0%
	ATTCCGGTGTCTGTCAAAGA+AGG	+	21207:12407-12426	MS.gene031197:CDS	45.0%
	CTGGATCGAGAGACAAATCT+GGG	+	21207:12378-12397	MS.gene031197:CDS	45.0%
	CTTCTCAGGGAGAAGAATAC+CGG	-	21207:12228-12247	None:intergenic	45.0%
	TCACCTTCTTTGACAGACAC+CGG	-	21207:12413-12432	None:intergenic	45.0%
	TCAGGAAAGGTTGTAGCTGT+TGG	+	21207:12353-12372	MS.gene031197:CDS	45.0%
!	TGATTTTCTCGACGAGGATG+CGG	-	21207:12184-12203	None:intergenic	45.0%
	AAACCTGGGAGGTCTTCTCA+GGG	-	21207:12241-12260	None:intergenic	50.0%
	AAGGTTGTAGCTGTTGGACC+TGG	+	21207:12359-12378	MS.gene031197:CDS	50.0%
	CACGTTCTCTTGCCGGAATA+TGG	+	21207:12434-12453	MS.gene031197:CDS	50.0%
	CATATTCCGGCAAGAGAACG+TGG	-	21207:12436-12455	None:intergenic	50.0%
	CCAGATTTGTCTCTCGATCC+AGG	-	21207:12380-12399	None:intergenic	50.0%
	CCTGGATCGAGAGACAAATC+TGG	+	21207:12377-12396	MS.gene031197:CDS	50.0%
	GTTCTCTTGCCGGAATATGG+TGG	+	21207:12437-12456	MS.gene031197:CDS	50.0%
!	GAGGGATGATTTTCTCGACG+AGG	-	21207:12190-12209	None:intergenic	50.0%
	AAGAATACCGGCGGAGGTCT+TGG	-	21207:12216-12235	None:intergenic	55.0%
	ACGAGGATGCGGTTGAAGGT+TGG	-	21207:12173-12192	None:intergenic	55.0%
	AGGTGACCACGTTCTCTTGC+CGG	+	21207:12427-12446	MS.gene031197:CDS	55.0%
	CAAACCTGGGAGGTCTTCTC+AGG	-	21207:12242-12261	None:intergenic	55.0%
	CTCAGGGAGAAGAATACCGG+CGG	-	21207:12225-12244	None:intergenic	55.0%
	AGGGAGAAGAATACCGGCGG+AGG	-	21207:12222-12241	None:intergenic	60.0%
	ATACCGGCGGAGGTCTTGGA+AGG	-	21207:12212-12231	None:intergenic	60.0%
	CTCGACGAGGATGCGGTTGA+AGG	-	21207:12177-12196	None:intergenic	60.0%
	TCTCCCTGAGAAGACCTCCC+AGG	+	21207:12235-12254	MS.gene031197:CDS	60.0%
	CCTCCTTCCAAGACCTCCGC+CGG	+	21207:12206-12225	MS.gene031197:CDS	65.0%
	CGGCGGAGGTCTTGGAAGGA+GGG	-	21207:12208-12227	None:intergenic	65.0%
	CCGGCGGAGGTCTTGGAAGG+AGG	-	21207:12209-12228	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
21207	gene	12152	12535	12152	ID=MS.gene031197
21207	mRNA	12152	12535	12152	ID=MS.gene031197.t1;Parent=MS.gene031197
21207	exon	12152	12256	12152	ID=MS.gene031197.t1.exon1;Parent=MS.gene031197.t1
21207	CDS	12152	12256	12152	ID=cds.MS.gene031197.t1;Parent=MS.gene031197.t1
21207	exon	12347	12535	12347	ID=MS.gene031197.t1.exon2;Parent=MS.gene031197.t1
21207	CDS	12347	12535	12347	ID=cds.MS.gene031197.t1;Parent=MS.gene031197.t1

Gene Sequence

>MS.gene031197

ATGGCTAAGCGTTTGATTCCAACCTTCAACCGCATCCTCGTCGAGAAAATCATCCCTCCTTCCAAGACCTCCGCCGGTATTCTTCTCCCTGAGAAGACCTCCCAGGTTTGTAGAAACTCTCTTCTTCATTTTTTTTATATATGTATGTCTCTGTGTTAATCGATTTGGATTTAATTGATTCTGGTTTGAAAACAGCTTAATTCAGGAAAGGTTGTAGCTGTTGGACCTGGATCGAGAGACAAATCTGGGAATTTGATTCCGGTGTCTGTCAAAGAAGGTGACCACGTTCTCTTGCCGGAATATGGTGGCTCTCAAATTAAGCTTGATGACAAAGAGTTTCATTTGTTTAGGGATGAGGACATTTTGGGCATTCTGCGTGATTGA

Protein sequence

>MS.gene031197.t1

MAKRLIPTFNRILVEKIIPPSKTSAGILLPEKTSQLNSGKVVAVGPGSRDKSGNLIPVSVKEGDHVLLPEYGGSQIKLDDKEFHLFRDEDILGILRD