Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75317.t1 | XP_013461973.1 | 100 | 97 | 0 | 0 | 1 | 97 | 1 | 97 | 8.60E-46 | 192.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75317.t1 | P34893 | 75.3 | 97 | 24 | 0 | 1 | 97 | 1 | 97 | 3.7e-37 | 155.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75317.t1 | G7ZWL8 | 100.0 | 97 | 0 | 0 | 1 | 97 | 1 | 97 | 6.2e-46 | 192.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene24509 | MS.gene75317 | PPI |
| MS.gene051662 | MS.gene75317 | PPI |
| MS.gene75317 | MS.gene039040 | PPI |
| MS.gene051663 | MS.gene75317 | PPI |
| MS.gene031197 | MS.gene75317 | PPI |
| MS.gene031195 | MS.gene75317 | PPI |
| MS.gene75317 | MS.gene75319 | PPI |
| MS.gene005697 | MS.gene75317 | PPI |
| MS.gene045566 | MS.gene75317 | PPI |
| MS.gene75317 | MS.gene031197 | PPI |
| MS.gene75317 | MS.gene66497 | PPI |
| MS.gene21357 | MS.gene75317 | PPI |
| MS.gene75317 | MS.gene00537 | PPI |
| MS.gene66498 | MS.gene75317 | PPI |
| MS.gene006641 | MS.gene75317 | PPI |
| MS.gene92186 | MS.gene75317 | PPI |
| MS.gene24508 | MS.gene75317 | PPI |
| MS.gene005698 | MS.gene75317 | PPI |
| MS.gene21583 | MS.gene75317 | PPI |
| MS.gene40328 | MS.gene75317 | PPI |
| MS.gene29382 | MS.gene75317 | PPI |
| MS.gene074000 | MS.gene75317 | PPI |
| MS.gene70655 | MS.gene75317 | PPI |
| MS.gene75317 | MS.gene00538 | PPI |
| MS.gene75317 | MS.gene25990 | PPI |
| MS.gene21355 | MS.gene75317 | PPI |
| MS.gene75317 | MS.gene21581 | PPI |
| MS.gene75317 | MS.gene40328 | PPI |
| MS.gene022326 | MS.gene75317 | PPI |
| MS.gene014672 | MS.gene75317 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75317.t1 | MTR_3g110030 | 100.000 | 97 | 0 | 0 | 1 | 97 | 1 | 97 | 9.29e-66 | 193 |
| MS.gene75317.t1 | MTR_3g077350 | 84.706 | 85 | 13 | 0 | 1 | 85 | 1 | 85 | 2.00e-48 | 149 |
| MS.gene75317.t1 | MTR_7g089300 | 74.227 | 97 | 25 | 0 | 1 | 97 | 1 | 97 | 3.13e-48 | 149 |
| MS.gene75317.t1 | MTR_3g089960 | 85.714 | 42 | 6 | 0 | 56 | 97 | 212 | 253 | 2.84e-19 | 79.3 |
| MS.gene75317.t1 | MTR_3g077580 | 86.486 | 37 | 5 | 0 | 1 | 37 | 1 | 37 | 1.21e-16 | 68.9 |
| MS.gene75317.t1 | MTR_2g043230 | 38.947 | 95 | 56 | 2 | 3 | 95 | 159 | 253 | 1.45e-11 | 58.5 |
| MS.gene75317.t1 | MTR_4g124910 | 37.778 | 90 | 54 | 2 | 7 | 95 | 64 | 152 | 3.05e-11 | 57.8 |
| MS.gene75317.t1 | MTR_4g124910 | 37.895 | 95 | 57 | 2 | 3 | 95 | 158 | 252 | 8.06e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75317.t1 | AT1G23100 | 75.258 | 97 | 24 | 0 | 1 | 97 | 1 | 97 | 7.18e-52 | 158 |
| MS.gene75317.t1 | AT1G14980 | 75.258 | 97 | 24 | 0 | 1 | 97 | 1 | 97 | 5.22e-51 | 155 |
| MS.gene75317.t1 | AT1G14980 | 75.610 | 82 | 20 | 0 | 1 | 82 | 1 | 82 | 5.04e-41 | 130 |
| MS.gene75317.t1 | AT5G20720 | 36.842 | 95 | 58 | 2 | 3 | 95 | 158 | 252 | 3.53e-11 | 57.8 |
| MS.gene75317.t1 | AT5G20720 | 36.842 | 95 | 58 | 2 | 3 | 95 | 158 | 252 | 3.53e-11 | 57.8 |
| MS.gene75317.t1 | AT5G20720 | 36.842 | 95 | 58 | 2 | 3 | 95 | 158 | 252 | 3.53e-11 | 57.8 |
| MS.gene75317.t1 | AT5G20720 | 36.842 | 95 | 58 | 2 | 3 | 95 | 158 | 252 | 3.53e-11 | 57.8 |
Find 33 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTAGGGATGAGGACATTT+TGG | 0.181417 | 3.3:+90315970 | MS.gene75317:CDS |
| ACAAAGAGTTTCATTTGTTT+AGG | 0.264498 | 3.3:+90315954 | MS.gene75317:CDS |
| GTTTGAAAACAGCTTAATTC+AGG | 0.277309 | 3.3:+90315809 | MS.gene75317:intron |
| CAAAGAGTTTCATTTGTTTA+GGG | 0.318510 | 3.3:+90315955 | MS.gene75317:CDS |
| CACGTTCTCTTGCCGGAATA+TGG | 0.350941 | 3.3:+90315908 | MS.gene75317:CDS |
| CTCGACGAGGATGCGGTTGA+AGG | 0.354705 | 3.3:-90315649 | None:intergenic |
| CAAACCTGGGAGGTCTTCTC+AGG | 0.357034 | 3.3:-90315714 | None:intergenic |
| AAGAATACCGGCGGAGGTCT+TGG | 0.361115 | 3.3:-90315688 | None:intergenic |
| ATTTGAGAGCCACCATATTC+CGG | 0.366812 | 3.3:-90315920 | None:intergenic |
| CAAATCTGGGAATTTGATTC+CGG | 0.387419 | 3.3:+90315865 | MS.gene75317:CDS |
| CCTGGATCGAGAGACAAATC+TGG | 0.418950 | 3.3:+90315851 | MS.gene75317:CDS |
| AGGTGACCACGTTCTCTTGC+CGG | 0.420998 | 3.3:+90315901 | MS.gene75317:CDS |
| GTTTCATTTGTTTAGGGATG+AGG | 0.443213 | 3.3:+90315961 | MS.gene75317:CDS |
| ACGAGGATGCGGTTGAAGGT+TGG | 0.459998 | 3.3:-90315645 | None:intergenic |
| CCAGATTTGTCTCTCGATCC+AGG | 0.466866 | 3.3:-90315851 | None:intergenic |
| TCAGGAAAGGTTGTAGCTGT+TGG | 0.477328 | 3.3:+90315827 | MS.gene75317:CDS |
| TCTCCCTGAGAAGACCTCCC+AGG | 0.483550 | 3.3:+90315710 | MS.gene75317:CDS |
| ATTCCGGTGTCTGTCAAAGA+AGG | 0.501127 | 3.3:+90315881 | MS.gene75317:CDS |
| CCGGCGGAGGTCTTGGAAGG+AGG | 0.529929 | 3.3:-90315681 | None:intergenic |
| AAAACAGCTTAATTCAGGAA+AGG | 0.534414 | 3.3:+90315814 | MS.gene75317:intron |
| CGGCGGAGGTCTTGGAAGGA+GGG | 0.543498 | 3.3:-90315680 | None:intergenic |
| AAGGTTGTAGCTGTTGGACC+TGG | 0.547728 | 3.3:+90315833 | MS.gene75317:CDS |
| CTTCTCAGGGAGAAGAATAC+CGG | 0.548614 | 3.3:-90315700 | None:intergenic |
| CTGGATCGAGAGACAAATCT+GGG | 0.579563 | 3.3:+90315852 | MS.gene75317:CDS |
| CCTCCTTCCAAGACCTCCGC+CGG | 0.595748 | 3.3:+90315681 | MS.gene75317:CDS |
| ATACCGGCGGAGGTCTTGGA+AGG | 0.597082 | 3.3:-90315684 | None:intergenic |
| GTTCTCTTGCCGGAATATGG+TGG | 0.611791 | 3.3:+90315911 | MS.gene75317:CDS |
| TCACCTTCTTTGACAGACAC+CGG | 0.618503 | 3.3:-90315884 | None:intergenic |
| AGGGAGAAGAATACCGGCGG+AGG | 0.620138 | 3.3:-90315694 | None:intergenic |
| CATATTCCGGCAAGAGAACG+TGG | 0.633016 | 3.3:-90315907 | None:intergenic |
| AAACCTGGGAGGTCTTCTCA+GGG | 0.670497 | 3.3:-90315713 | None:intergenic |
| CTCAGGGAGAAGAATACCGG+CGG | 0.675295 | 3.3:-90315697 | None:intergenic |
| AGAGTTTCTACAAACCTGGG+AGG | 0.716301 | 3.3:-90315724 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAAGAGTTTCATTTGTTT+AGG | + | chr3.3:90315954-90315973 | MS.gene75317:CDS | 25.0% |
| ! | CAAAGAGTTTCATTTGTTTA+GGG | + | chr3.3:90315955-90315974 | MS.gene75317:CDS | 25.0% |
| !! | GATTTGGATTTAATTGATTC+TGG | + | chr3.3:90315787-90315806 | MS.gene75317:intron | 25.0% |
| AAAACAGCTTAATTCAGGAA+AGG | + | chr3.3:90315814-90315833 | MS.gene75317:intron | 30.0% | |
| GTTTGAAAACAGCTTAATTC+AGG | + | chr3.3:90315809-90315828 | MS.gene75317:intron | 30.0% | |
| AAGAGAGTTTCTACAAACCT+GGG | - | chr3.3:90315730-90315749 | None:intergenic | 35.0% | |
| CAAATCTGGGAATTTGATTC+CGG | + | chr3.3:90315865-90315884 | MS.gene75317:CDS | 35.0% | |
| GTTTCATTTGTTTAGGGATG+AGG | + | chr3.3:90315961-90315980 | MS.gene75317:CDS | 35.0% | |
| TGTCTCTGTGTTAATCGATT+TGG | + | chr3.3:90315771-90315790 | MS.gene75317:intron | 35.0% | |
| ! | TTTAGGGATGAGGACATTTT+GGG | + | chr3.3:90315971-90315990 | MS.gene75317:CDS | 35.0% |
| ATTTGAGAGCCACCATATTC+CGG | - | chr3.3:90315923-90315942 | None:intergenic | 40.0% | |
| GAAGAGAGTTTCTACAAACC+TGG | - | chr3.3:90315731-90315750 | None:intergenic | 40.0% | |
| ! | GTTTAGGGATGAGGACATTT+TGG | + | chr3.3:90315970-90315989 | MS.gene75317:CDS | 40.0% |
| AGAGTTTCTACAAACCTGGG+AGG | - | chr3.3:90315727-90315746 | None:intergenic | 45.0% | |
| ATTCCGGTGTCTGTCAAAGA+AGG | + | chr3.3:90315881-90315900 | MS.gene75317:CDS | 45.0% | |
| CTGGATCGAGAGACAAATCT+GGG | + | chr3.3:90315852-90315871 | MS.gene75317:CDS | 45.0% | |
| CTTCTCAGGGAGAAGAATAC+CGG | - | chr3.3:90315703-90315722 | None:intergenic | 45.0% | |
| TCACCTTCTTTGACAGACAC+CGG | - | chr3.3:90315887-90315906 | None:intergenic | 45.0% | |
| TCAGGAAAGGTTGTAGCTGT+TGG | + | chr3.3:90315827-90315846 | MS.gene75317:CDS | 45.0% | |
| ! | TGATTTTCTCGACGAGGATG+CGG | - | chr3.3:90315659-90315678 | None:intergenic | 45.0% |
| AAACCTGGGAGGTCTTCTCA+GGG | - | chr3.3:90315716-90315735 | None:intergenic | 50.0% | |
| AAGGTTGTAGCTGTTGGACC+TGG | + | chr3.3:90315833-90315852 | MS.gene75317:CDS | 50.0% | |
| CACGTTCTCTTGCCGGAATA+TGG | + | chr3.3:90315908-90315927 | MS.gene75317:CDS | 50.0% | |
| CATATTCCGGCAAGAGAACG+TGG | - | chr3.3:90315910-90315929 | None:intergenic | 50.0% | |
| CCAGATTTGTCTCTCGATCC+AGG | - | chr3.3:90315854-90315873 | None:intergenic | 50.0% | |
| CCTGGATCGAGAGACAAATC+TGG | + | chr3.3:90315851-90315870 | MS.gene75317:CDS | 50.0% | |
| GTTCTCTTGCCGGAATATGG+TGG | + | chr3.3:90315911-90315930 | MS.gene75317:CDS | 50.0% | |
| ! | GAGGGATGATTTTCTCGACG+AGG | - | chr3.3:90315665-90315684 | None:intergenic | 50.0% |
| AAGAATACCGGCGGAGGTCT+TGG | - | chr3.3:90315691-90315710 | None:intergenic | 55.0% | |
| ACGAGGATGCGGTTGAAGGT+TGG | - | chr3.3:90315648-90315667 | None:intergenic | 55.0% | |
| AGGTGACCACGTTCTCTTGC+CGG | + | chr3.3:90315901-90315920 | MS.gene75317:CDS | 55.0% | |
| CAAACCTGGGAGGTCTTCTC+AGG | - | chr3.3:90315717-90315736 | None:intergenic | 55.0% | |
| CTCAGGGAGAAGAATACCGG+CGG | - | chr3.3:90315700-90315719 | None:intergenic | 55.0% | |
| AGGGAGAAGAATACCGGCGG+AGG | - | chr3.3:90315697-90315716 | None:intergenic | 60.0% | |
| ATACCGGCGGAGGTCTTGGA+AGG | - | chr3.3:90315687-90315706 | None:intergenic | 60.0% | |
| CTCGACGAGGATGCGGTTGA+AGG | - | chr3.3:90315652-90315671 | None:intergenic | 60.0% | |
| TCTCCCTGAGAAGACCTCCC+AGG | + | chr3.3:90315710-90315729 | MS.gene75317:CDS | 60.0% | |
| CCTCCTTCCAAGACCTCCGC+CGG | + | chr3.3:90315681-90315700 | MS.gene75317:CDS | 65.0% | |
| CGGCGGAGGTCTTGGAAGGA+GGG | - | chr3.3:90315683-90315702 | None:intergenic | 65.0% | |
| CCGGCGGAGGTCTTGGAAGG+AGG | - | chr3.3:90315684-90315703 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 90315627 | 90316009 | 90315627 | ID=MS.gene75317 |
| chr3.3 | mRNA | 90315627 | 90316009 | 90315627 | ID=MS.gene75317.t1;Parent=MS.gene75317 |
| chr3.3 | exon | 90315627 | 90315731 | 90315627 | ID=MS.gene75317.t1.exon1;Parent=MS.gene75317.t1 |
| chr3.3 | CDS | 90315627 | 90315731 | 90315627 | ID=cds.MS.gene75317.t1;Parent=MS.gene75317.t1 |
| chr3.3 | exon | 90315821 | 90316009 | 90315821 | ID=MS.gene75317.t1.exon2;Parent=MS.gene75317.t1 |
| chr3.3 | CDS | 90315821 | 90316009 | 90315821 | ID=cds.MS.gene75317.t1;Parent=MS.gene75317.t1 |
| Gene Sequence |
| Protein sequence |
