Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40328.t1 | XP_013461973.1 | 100 | 97 | 0 | 0 | 1 | 97 | 1 | 97 | 8.60E-46 | 192.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40328.t1 | P34893 | 75.3 | 97 | 24 | 0 | 1 | 97 | 1 | 97 | 3.7e-37 | 155.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40328.t1 | G7ZWL8 | 100.0 | 97 | 0 | 0 | 1 | 97 | 1 | 97 | 6.2e-46 | 192.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene24508 | MS.gene40328 | PPI |
| MS.gene005698 | MS.gene40328 | PPI |
| MS.gene66498 | MS.gene40328 | PPI |
| MS.gene40328 | MS.gene00537 | PPI |
| MS.gene70655 | MS.gene40328 | PPI |
| MS.gene40328 | MS.gene00538 | PPI |
| MS.gene40328 | MS.gene25990 | PPI |
| MS.gene92186 | MS.gene40328 | PPI |
| MS.gene40328 | MS.gene21581 | PPI |
| MS.gene21583 | MS.gene40328 | PPI |
| MS.gene29382 | MS.gene40328 | PPI |
| MS.gene014672 | MS.gene40328 | PPI |
| MS.gene40328 | MS.gene039040 | PPI |
| MS.gene074000 | MS.gene40328 | PPI |
| MS.gene40328 | MS.gene75319 | PPI |
| MS.gene21355 | MS.gene40328 | PPI |
| MS.gene005697 | MS.gene40328 | PPI |
| MS.gene022326 | MS.gene40328 | PPI |
| MS.gene40328 | MS.gene75317 | PPI |
| MS.gene40328 | MS.gene031197 | PPI |
| MS.gene40328 | MS.gene66497 | PPI |
| MS.gene24509 | MS.gene40328 | PPI |
| MS.gene051662 | MS.gene40328 | PPI |
| MS.gene21357 | MS.gene40328 | PPI |
| MS.gene051663 | MS.gene40328 | PPI |
| MS.gene031197 | MS.gene40328 | PPI |
| MS.gene031195 | MS.gene40328 | PPI |
| MS.gene006641 | MS.gene40328 | PPI |
| MS.gene75317 | MS.gene40328 | PPI |
| MS.gene045566 | MS.gene40328 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40328.t1 | MTR_3g110030 | 100.000 | 97 | 0 | 0 | 1 | 97 | 1 | 97 | 9.29e-66 | 193 |
| MS.gene40328.t1 | MTR_3g077350 | 84.706 | 85 | 13 | 0 | 1 | 85 | 1 | 85 | 2.00e-48 | 149 |
| MS.gene40328.t1 | MTR_7g089300 | 74.227 | 97 | 25 | 0 | 1 | 97 | 1 | 97 | 3.13e-48 | 149 |
| MS.gene40328.t1 | MTR_3g089960 | 85.714 | 42 | 6 | 0 | 56 | 97 | 212 | 253 | 2.84e-19 | 79.3 |
| MS.gene40328.t1 | MTR_3g077580 | 86.486 | 37 | 5 | 0 | 1 | 37 | 1 | 37 | 1.21e-16 | 68.9 |
| MS.gene40328.t1 | MTR_2g043230 | 38.947 | 95 | 56 | 2 | 3 | 95 | 159 | 253 | 1.45e-11 | 58.5 |
| MS.gene40328.t1 | MTR_4g124910 | 37.778 | 90 | 54 | 2 | 7 | 95 | 64 | 152 | 3.05e-11 | 57.8 |
| MS.gene40328.t1 | MTR_4g124910 | 37.895 | 95 | 57 | 2 | 3 | 95 | 158 | 252 | 8.06e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40328.t1 | AT1G23100 | 75.258 | 97 | 24 | 0 | 1 | 97 | 1 | 97 | 7.18e-52 | 158 |
| MS.gene40328.t1 | AT1G14980 | 75.258 | 97 | 24 | 0 | 1 | 97 | 1 | 97 | 5.22e-51 | 155 |
| MS.gene40328.t1 | AT1G14980 | 75.610 | 82 | 20 | 0 | 1 | 82 | 1 | 82 | 5.04e-41 | 130 |
| MS.gene40328.t1 | AT5G20720 | 36.842 | 95 | 58 | 2 | 3 | 95 | 158 | 252 | 3.53e-11 | 57.8 |
| MS.gene40328.t1 | AT5G20720 | 36.842 | 95 | 58 | 2 | 3 | 95 | 158 | 252 | 3.53e-11 | 57.8 |
| MS.gene40328.t1 | AT5G20720 | 36.842 | 95 | 58 | 2 | 3 | 95 | 158 | 252 | 3.53e-11 | 57.8 |
| MS.gene40328.t1 | AT5G20720 | 36.842 | 95 | 58 | 2 | 3 | 95 | 158 | 252 | 3.53e-11 | 57.8 |
Find 33 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTAGGGATGAGGACATTT+TGG | 0.181417 | 3.4:+94890845 | MS.gene40328:CDS |
| ACAAAGAGTTTCATTTGTTT+AGG | 0.264498 | 3.4:+94890829 | MS.gene40328:CDS |
| GTTTGAAAACAGCTTAATTC+AGG | 0.277309 | 3.4:+94890684 | MS.gene40328:intron |
| CAAAGAGTTTCATTTGTTTA+GGG | 0.318510 | 3.4:+94890830 | MS.gene40328:CDS |
| CACGTTCTCTTGCCGGAATA+TGG | 0.350941 | 3.4:+94890783 | MS.gene40328:CDS |
| CTCGACGAGGATGCGGTTGA+AGG | 0.354705 | 3.4:-94890524 | None:intergenic |
| CAAACCTGGGAGGTCTTCTC+AGG | 0.357034 | 3.4:-94890589 | None:intergenic |
| AAGAATACCGGCGGAGGTCT+TGG | 0.361115 | 3.4:-94890563 | None:intergenic |
| ATTTGAGAGCCACCATATTC+CGG | 0.366812 | 3.4:-94890795 | None:intergenic |
| CAAATCTGGGAATTTGATTC+CGG | 0.387419 | 3.4:+94890740 | MS.gene40328:CDS |
| CCTGGATCGAGAGACAAATC+TGG | 0.418950 | 3.4:+94890726 | MS.gene40328:CDS |
| AGGTGACCACGTTCTCTTGC+CGG | 0.420998 | 3.4:+94890776 | MS.gene40328:CDS |
| GTTTCATTTGTTTAGGGATG+AGG | 0.443213 | 3.4:+94890836 | MS.gene40328:CDS |
| ACGAGGATGCGGTTGAAGGT+TGG | 0.459998 | 3.4:-94890520 | None:intergenic |
| CCAGATTTGTCTCTCGATCC+AGG | 0.466866 | 3.4:-94890726 | None:intergenic |
| TCAGGAAAGGTTGTAGCTGT+TGG | 0.477328 | 3.4:+94890702 | MS.gene40328:CDS |
| TCTCCCTGAGAAGACCTCCC+AGG | 0.483550 | 3.4:+94890585 | MS.gene40328:CDS |
| ATTCCGGTGTCTGTCAAAGA+AGG | 0.501127 | 3.4:+94890756 | MS.gene40328:CDS |
| CCGGCGGAGGTCTTGGAAGG+AGG | 0.529929 | 3.4:-94890556 | None:intergenic |
| AAAACAGCTTAATTCAGGAA+AGG | 0.534414 | 3.4:+94890689 | MS.gene40328:intron |
| CGGCGGAGGTCTTGGAAGGA+GGG | 0.543498 | 3.4:-94890555 | None:intergenic |
| AAGGTTGTAGCTGTTGGACC+TGG | 0.547728 | 3.4:+94890708 | MS.gene40328:CDS |
| CTTCTCAGGGAGAAGAATAC+CGG | 0.548614 | 3.4:-94890575 | None:intergenic |
| CTGGATCGAGAGACAAATCT+GGG | 0.579563 | 3.4:+94890727 | MS.gene40328:CDS |
| CCTCCTTCCAAGACCTCCGC+CGG | 0.595748 | 3.4:+94890556 | MS.gene40328:CDS |
| ATACCGGCGGAGGTCTTGGA+AGG | 0.597082 | 3.4:-94890559 | None:intergenic |
| GTTCTCTTGCCGGAATATGG+TGG | 0.611791 | 3.4:+94890786 | MS.gene40328:CDS |
| TCACCTTCTTTGACAGACAC+CGG | 0.618503 | 3.4:-94890759 | None:intergenic |
| AGGGAGAAGAATACCGGCGG+AGG | 0.620138 | 3.4:-94890569 | None:intergenic |
| CATATTCCGGCAAGAGAACG+TGG | 0.633016 | 3.4:-94890782 | None:intergenic |
| AAACCTGGGAGGTCTTCTCA+GGG | 0.670497 | 3.4:-94890588 | None:intergenic |
| CTCAGGGAGAAGAATACCGG+CGG | 0.675295 | 3.4:-94890572 | None:intergenic |
| AGAGTTTCTACAAACCTGGG+AGG | 0.716301 | 3.4:-94890599 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAAGAGTTTCATTTGTTT+AGG | + | chr3.4:94890829-94890848 | MS.gene40328:CDS | 25.0% |
| ! | CAAAGAGTTTCATTTGTTTA+GGG | + | chr3.4:94890830-94890849 | MS.gene40328:CDS | 25.0% |
| !! | GATTTGGATTTAATTGATTC+TGG | + | chr3.4:94890662-94890681 | MS.gene40328:intron | 25.0% |
| AAAACAGCTTAATTCAGGAA+AGG | + | chr3.4:94890689-94890708 | MS.gene40328:intron | 30.0% | |
| GTTTGAAAACAGCTTAATTC+AGG | + | chr3.4:94890684-94890703 | MS.gene40328:intron | 30.0% | |
| AAGAGAGTTTCTACAAACCT+GGG | - | chr3.4:94890605-94890624 | None:intergenic | 35.0% | |
| CAAATCTGGGAATTTGATTC+CGG | + | chr3.4:94890740-94890759 | MS.gene40328:CDS | 35.0% | |
| GTTTCATTTGTTTAGGGATG+AGG | + | chr3.4:94890836-94890855 | MS.gene40328:CDS | 35.0% | |
| TGTCTCTGTGTTAATCGATT+TGG | + | chr3.4:94890646-94890665 | MS.gene40328:intron | 35.0% | |
| ! | TTTAGGGATGAGGACATTTT+GGG | + | chr3.4:94890846-94890865 | MS.gene40328:CDS | 35.0% |
| ATTTGAGAGCCACCATATTC+CGG | - | chr3.4:94890798-94890817 | None:intergenic | 40.0% | |
| GAAGAGAGTTTCTACAAACC+TGG | - | chr3.4:94890606-94890625 | None:intergenic | 40.0% | |
| ! | GTTTAGGGATGAGGACATTT+TGG | + | chr3.4:94890845-94890864 | MS.gene40328:CDS | 40.0% |
| AGAGTTTCTACAAACCTGGG+AGG | - | chr3.4:94890602-94890621 | None:intergenic | 45.0% | |
| ATTCCGGTGTCTGTCAAAGA+AGG | + | chr3.4:94890756-94890775 | MS.gene40328:CDS | 45.0% | |
| CTGGATCGAGAGACAAATCT+GGG | + | chr3.4:94890727-94890746 | MS.gene40328:CDS | 45.0% | |
| CTTCTCAGGGAGAAGAATAC+CGG | - | chr3.4:94890578-94890597 | None:intergenic | 45.0% | |
| TCACCTTCTTTGACAGACAC+CGG | - | chr3.4:94890762-94890781 | None:intergenic | 45.0% | |
| TCAGGAAAGGTTGTAGCTGT+TGG | + | chr3.4:94890702-94890721 | MS.gene40328:CDS | 45.0% | |
| ! | TGATTTTCTCGACGAGGATG+CGG | - | chr3.4:94890534-94890553 | None:intergenic | 45.0% |
| AAACCTGGGAGGTCTTCTCA+GGG | - | chr3.4:94890591-94890610 | None:intergenic | 50.0% | |
| AAGGTTGTAGCTGTTGGACC+TGG | + | chr3.4:94890708-94890727 | MS.gene40328:CDS | 50.0% | |
| CACGTTCTCTTGCCGGAATA+TGG | + | chr3.4:94890783-94890802 | MS.gene40328:CDS | 50.0% | |
| CATATTCCGGCAAGAGAACG+TGG | - | chr3.4:94890785-94890804 | None:intergenic | 50.0% | |
| CCAGATTTGTCTCTCGATCC+AGG | - | chr3.4:94890729-94890748 | None:intergenic | 50.0% | |
| CCTGGATCGAGAGACAAATC+TGG | + | chr3.4:94890726-94890745 | MS.gene40328:CDS | 50.0% | |
| GTTCTCTTGCCGGAATATGG+TGG | + | chr3.4:94890786-94890805 | MS.gene40328:CDS | 50.0% | |
| ! | GAGGGATGATTTTCTCGACG+AGG | - | chr3.4:94890540-94890559 | None:intergenic | 50.0% |
| AAGAATACCGGCGGAGGTCT+TGG | - | chr3.4:94890566-94890585 | None:intergenic | 55.0% | |
| ACGAGGATGCGGTTGAAGGT+TGG | - | chr3.4:94890523-94890542 | None:intergenic | 55.0% | |
| AGGTGACCACGTTCTCTTGC+CGG | + | chr3.4:94890776-94890795 | MS.gene40328:CDS | 55.0% | |
| CAAACCTGGGAGGTCTTCTC+AGG | - | chr3.4:94890592-94890611 | None:intergenic | 55.0% | |
| CTCAGGGAGAAGAATACCGG+CGG | - | chr3.4:94890575-94890594 | None:intergenic | 55.0% | |
| AGGGAGAAGAATACCGGCGG+AGG | - | chr3.4:94890572-94890591 | None:intergenic | 60.0% | |
| ATACCGGCGGAGGTCTTGGA+AGG | - | chr3.4:94890562-94890581 | None:intergenic | 60.0% | |
| CTCGACGAGGATGCGGTTGA+AGG | - | chr3.4:94890527-94890546 | None:intergenic | 60.0% | |
| TCTCCCTGAGAAGACCTCCC+AGG | + | chr3.4:94890585-94890604 | MS.gene40328:CDS | 60.0% | |
| CCTCCTTCCAAGACCTCCGC+CGG | + | chr3.4:94890556-94890575 | MS.gene40328:CDS | 65.0% | |
| CGGCGGAGGTCTTGGAAGGA+GGG | - | chr3.4:94890558-94890577 | None:intergenic | 65.0% | |
| CCGGCGGAGGTCTTGGAAGG+AGG | - | chr3.4:94890559-94890578 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 94890502 | 94890884 | 94890502 | ID=MS.gene40328 |
| chr3.4 | mRNA | 94890502 | 94890884 | 94890502 | ID=MS.gene40328.t1;Parent=MS.gene40328 |
| chr3.4 | exon | 94890502 | 94890606 | 94890502 | ID=MS.gene40328.t1.exon1;Parent=MS.gene40328.t1 |
| chr3.4 | CDS | 94890502 | 94890606 | 94890502 | ID=cds.MS.gene40328.t1;Parent=MS.gene40328.t1 |
| chr3.4 | exon | 94890696 | 94890884 | 94890696 | ID=MS.gene40328.t1.exon2;Parent=MS.gene40328.t1 |
| chr3.4 | CDS | 94890696 | 94890884 | 94890696 | ID=cds.MS.gene40328.t1;Parent=MS.gene40328.t1 |
| Gene Sequence |
| Protein sequence |
