AlfalfaGEDB | MS.gene025139

M. sativa cultivar XinJiangDaYe / MS.gene025139

KEGG: K02218
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025139.t1	XP_003595230.1	95.1	472	17	2	1	467	1	471	5.60E-252	879.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025139.t1	P42158	68.5	464	125	3	1	462	1	445	2.1e-178	626.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025139.t1	A2Q686	95.1	472	17	2	1	467	1	471	4.1e-252	879.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene057000	MS.gene025139	PPI
MS.gene038078	MS.gene025139	PPI
MS.gene025139	MS.gene02469	PPI
MS.gene025139	MS.gene85233	PPI
MS.gene051853	MS.gene025139	PPI
MS.gene025139	MS.gene89575	PPI
MS.gene061465	MS.gene025139	PPI
MS.gene025139	MS.gene37374	PPI
MS.gene07819	MS.gene025139	PPI
MS.gene39623	MS.gene025139	PPI
MS.gene049095	MS.gene025139	PPI
MS.gene015777	MS.gene025139	PPI
MS.gene23970	MS.gene025139	PPI
MS.gene012266	MS.gene025139	PPI
MS.gene21700	MS.gene025139	PPI
MS.gene036055	MS.gene025139	PPI
MS.gene26532	MS.gene025139	PPI
MS.gene47479	MS.gene025139	PPI
MS.gene04733	MS.gene025139	PPI
MS.gene008767	MS.gene025139	PPI
MS.gene059592	MS.gene025139	PPI
MS.gene025139	MS.gene052689	PPI
MS.gene011236	MS.gene025139	PPI
MS.gene025139	MS.gene05889	PPI
MS.gene57575	MS.gene025139	PPI
MS.gene051961	MS.gene025139	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025139.t1	MTR_2g039930	96.610	472	10	2	1	467	1	471	0.0	939
MS.gene025139.t1	MTR_2g039930	95.908	391	10	2	82	467	1	390	0.0	772
MS.gene025139.t1	MTR_4g126010	77.419	465	85	5	1	462	1	448	0.0	709
MS.gene025139.t1	MTR_2g099670	70.851	470	129	4	1	462	1	470	0.0	681
MS.gene025139.t1	MTR_8g018310	70.000	470	132	5	1	462	1	469	0.0	656
MS.gene025139.t1	MTR_8g018310	78.649	370	77	2	1	369	1	369	0.0	614
MS.gene025139.t1	MTR_2g099670	69.287	407	117	4	64	462	12	418	0.0	576
MS.gene025139.t1	MTR_1g069720	54.412	476	179	7	1	461	1	453	1.20e-179	510
MS.gene025139.t1	MTR_1g042280	73.502	317	82	1	1	315	2	318	2.14e-178	507
MS.gene025139.t1	MTR_7g097160	66.667	351	107	3	1	346	1	346	7.83e-177	504
MS.gene025139.t1	MTR_8g102930	58.525	434	163	5	1	428	1	423	1.19e-173	494
MS.gene025139.t1	MTR_1g075870	73.064	297	80	0	1	297	2	298	6.23e-173	488
MS.gene025139.t1	MTR_1g075870	73.064	297	80	0	1	297	2	298	3.68e-172	492
MS.gene025139.t1	MTR_6g087070	73.579	299	79	0	1	299	1	299	6.19e-171	487
MS.gene025139.t1	MTR_1g004990	67.188	320	98	1	1	313	1	320	4.88e-163	467
MS.gene025139.t1	MTR_3g110180	59.686	382	143	4	1	381	1	372	4.94e-163	467
MS.gene025139.t1	MTR_3g110180	59.686	382	142	5	1	381	1	371	2.56e-161	463
MS.gene025139.t1	MTR_0492s0030	60.372	376	136	5	1	370	1	369	2.14e-157	451
MS.gene025139.t1	MTR_0543s0020	60.372	376	136	5	1	370	1	369	2.14e-157	451
MS.gene025139.t1	MTR_1g042280	74.460	278	69	1	40	315	9	286	3.07e-157	448
MS.gene025139.t1	MTR_1g075870	75.000	240	60	0	58	297	2	241	5.18e-143	411
MS.gene025139.t1	MTR_7g097160	60.851	235	78	4	144	373	25	250	4.66e-93	286
MS.gene025139.t1	MTR_7g006030	37.809	283	159	5	9	277	37	316	8.90e-60	206
MS.gene025139.t1	MTR_3g110240	39.194	273	155	5	9	273	121	390	1.23e-57	202
MS.gene025139.t1	MTR_1g007150	38.462	273	157	5	9	273	124	393	2.77e-57	201
MS.gene025139.t1	MTR_7g085540	37.716	289	162	6	4	277	140	425	6.11e-57	200
MS.gene025139.t1	MTR_1g116210	37.410	278	162	5	9	277	119	393	3.55e-56	198
MS.gene025139.t1	MTR_8g014050	36.429	280	160	6	9	273	128	404	3.77e-51	184
MS.gene025139.t1	MTR_7g116400	52.703	74	28	2	82	148	3	76	4.36e-18	79.3
MS.gene025139.t1	MTR_5g022030	25.792	221	144	7	2	213	92	301	2.16e-13	72.8
MS.gene025139.t1	MTR_7g073520	28.968	252	154	12	3	243	141	378	2.26e-13	72.4
MS.gene025139.t1	MTR_5g022030	25.792	221	144	7	2	213	92	301	2.81e-13	72.4
MS.gene025139.t1	MTR_4g132860	26.389	288	172	11	11	282	82	345	1.05e-12	69.7
MS.gene025139.t1	MTR_1g105290	26.364	220	135	8	8	213	54	260	7.37e-12	68.2
MS.gene025139.t1	MTR_2g105010	29.091	220	130	10	7	215	12	216	7.67e-12	67.4
MS.gene025139.t1	MTR_1g105290	26.364	220	135	8	8	213	54	260	9.57e-12	67.8
MS.gene025139.t1	MTR_7g116390	24.766	214	145	5	8	215	15	218	1.22e-11	65.9
MS.gene025139.t1	MTR_1g105290	26.364	220	135	8	8	213	54	260	1.24e-11	67.0
MS.gene025139.t1	MTR_5g075060	29.787	235	134	12	8	230	11	226	1.45e-11	66.6
MS.gene025139.t1	MTR_3g466400	29.167	216	131	8	8	214	13	215	1.59e-11	66.6
MS.gene025139.t1	MTR_3g092230	29.046	241	124	13	3	221	132	347	2.29e-11	66.2
MS.gene025139.t1	MTR_1g101630	24.891	229	151	7	7	228	39	253	2.48e-11	66.2
MS.gene025139.t1	MTR_8g099095	31.628	215	123	10	9	213	66	266	3.17e-11	65.9
MS.gene025139.t1	MTR_1g052530	25.882	255	143	10	3	230	46	281	6.69e-11	64.3
MS.gene025139.t1	MTR_5g007590	38.095	84	38	2	71	148	53	128	7.00e-11	60.1
MS.gene025139.t1	MTR_1g052530	25.969	258	139	11	3	230	46	281	7.93e-11	64.7
MS.gene025139.t1	MTR_8g079790	26.446	242	132	9	3	221	119	337	8.76e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025139.t1	AT4G26100	68.750	464	124	4	1	462	1	445	0.0	654
MS.gene025139.t1	AT1G72710	67.516	471	132	6	1	458	1	463	0.0	624
MS.gene025139.t1	AT5G57015	66.026	468	124	6	1	467	1	434	0.0	622
MS.gene025139.t1	AT2G19470	59.915	469	121	4	1	462	1	409	0.0	557
MS.gene025139.t1	AT5G44100	75.758	297	72	0	1	297	1	297	1.82e-178	508
MS.gene025139.t1	AT5G44100	75.758	297	72	0	1	297	1	297	1.82e-178	508
MS.gene025139.t1	AT1G03930	58.482	448	159	7	1	429	1	440	3.88e-178	507
MS.gene025139.t1	AT3G23340	73.355	304	81	0	1	304	1	304	3.70e-177	504
MS.gene025139.t1	AT1G04440	62.304	382	127	3	1	365	1	382	3.20e-174	497
MS.gene025139.t1	AT4G28540	58.911	404	149	5	1	396	5	399	1.88e-172	493
MS.gene025139.t1	AT4G14340	58.795	415	150	5	1	396	7	419	1.25e-171	490
MS.gene025139.t1	AT5G43320	60.836	383	131	2	1	364	1	383	4.31e-171	490
MS.gene025139.t1	AT4G14340	72.727	297	81	0	1	297	7	303	5.62e-171	485
MS.gene025139.t1	AT4G28860	65.057	352	119	2	1	352	1	348	7.94e-171	485
MS.gene025139.t1	AT4G28860	59.158	404	158	3	1	401	1	400	1.57e-170	486
MS.gene025139.t1	AT4G28880	60.149	404	145	6	1	396	1	396	2.23e-170	485
MS.gene025139.t1	AT4G28880	60.149	404	145	6	1	396	1	396	2.23e-170	485
MS.gene025139.t1	AT4G28880	60.149	404	145	6	1	396	1	396	2.23e-170	485
MS.gene025139.t1	AT4G28880	60.149	404	145	6	1	396	1	396	2.23e-170	485
MS.gene025139.t1	AT5G43320	72.391	297	82	0	1	297	1	297	5.90e-168	478
MS.gene025139.t1	AT5G43320	72.391	297	82	0	1	297	1	297	5.90e-168	478
MS.gene025139.t1	AT3G23340	73.592	284	75	0	21	304	7	290	3.53e-164	470
MS.gene025139.t1	AT4G08800	65.677	303	72	2	1	303	1	271	6.58e-147	421
MS.gene025139.t1	AT2G25760	38.676	287	159	5	4	273	100	386	7.74e-61	211
MS.gene025139.t1	AT2G25760	38.676	287	156	6	4	273	100	383	2.37e-59	206
MS.gene025139.t1	AT3G03940	38.408	289	160	6	4	277	133	418	3.82e-57	201
MS.gene025139.t1	AT5G18190	38.062	289	161	6	4	277	123	408	8.66e-57	200
MS.gene025139.t1	AT5G18190	38.062	289	161	6	4	277	123	408	8.66e-57	200
MS.gene025139.t1	AT3G13670	36.749	283	162	5	9	277	137	416	1.12e-56	200
MS.gene025139.t1	AT2G25090	29.018	224	130	10	7	213	13	224	8.59e-12	67.4
MS.gene025139.t1	AT1G12580	24.686	239	159	7	7	238	42	266	9.59e-12	67.4
MS.gene025139.t1	AT1G61950	24.749	299	196	12	5	293	94	373	1.29e-11	67.0
MS.gene025139.t1	AT2G17890	24.766	214	145	7	7	213	106	310	1.51e-11	67.0
MS.gene025139.t1	AT3G06030	26.818	220	134	9	8	213	67	273	2.47e-11	66.2
MS.gene025139.t1	AT5G58380	30.000	220	126	11	7	214	10	213	2.98e-11	65.9
MS.gene025139.t1	AT4G23650	30.531	226	115	12	9	215	78	280	3.77e-11	65.5
MS.gene025139.t1	AT2G25880	25.738	237	148	9	2	230	31	247	3.89e-11	64.3
MS.gene025139.t1	AT1G62400	25.694	288	174	11	11	282	88	351	3.89e-11	65.1
MS.gene025139.t1	AT2G25880	25.738	237	148	9	2	230	18	234	4.05e-11	64.3
MS.gene025139.t1	AT2G38910	26.400	250	155	10	9	242	134	370	4.21e-11	65.5
MS.gene025139.t1	AT5G25110	27.679	224	134	10	3	214	37	244	4.56e-11	65.5
MS.gene025139.t1	AT2G25880	25.738	237	148	9	2	230	79	295	6.05e-11	64.3

Find 110 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTCCAAACCATTTAATATT+TGG	0.121812	2.4:+50113827	None:intergenic
CTGGGATTCCAAATATTAAA+TGG	0.220549	2.4:-50113835	MS.gene025139:CDS
AATATTTACAGAATTTAAAA+TGG	0.232763	2.4:+50110031	None:intergenic
TCATTCGGAGAGATCTATTT+AGG	0.249141	2.4:-50116790	MS.gene025139:intron
TCCATTATTGCCGGTCATTA+AGG	0.259862	2.4:-50111431	MS.gene025139:CDS
TAGGTTTACTGTATTGATTT+TGG	0.282532	2.4:-50113398	MS.gene025139:CDS
ACCTTAATGACCGGCAATAA+TGG	0.283364	2.4:+50111430	None:intergenic
CTTAATGCTGGAACTAGTTC+TGG	0.302221	2.4:-50111045	MS.gene025139:CDS
ATTCCAAATATTAAATGGTT+TGG	0.317069	2.4:-50113830	MS.gene025139:CDS
CCCATCAACACATTCCTTAC+AGG	0.339922	2.4:-50113346	MS.gene025139:intron
CTAGAAGATGTTACTCTCCT+TGG	0.347085	2.4:+50109709	None:intergenic
AGGGAAGAAGGGCGTCATTC+TGG	0.376963	2.4:-50110095	MS.gene025139:CDS
ATGTCTTGATGTACTTCCTC+AGG	0.377055	2.4:-50111768	MS.gene025139:CDS
GATAATTTCCTCATGGGCTT+GGG	0.378614	2.4:-50113521	MS.gene025139:CDS
AGATAATTTCCTCATGGGCT+TGG	0.386906	2.4:-50113522	MS.gene025139:CDS
AGCAGTAGCCGCAATGCATT+TGG	0.394778	2.4:-50109835	MS.gene025139:CDS
AGTCCGATTACATCTTTGAT+TGG	0.394843	2.4:-50111207	MS.gene025139:CDS
GGTCATTAAGGTTTGATGAT+AGG	0.395299	2.4:-50111419	MS.gene025139:CDS
AAGCCCATGAGGAAATTATC+TGG	0.401745	2.4:+50113524	None:intergenic
TTTGAATGGAACGTCGCGTT+GGG	0.404069	2.4:-50116849	None:intergenic
CATCATACTTCCATTATTGC+CGG	0.415822	2.4:-50111440	MS.gene025139:CDS
TCAAACCTTCACGAATAAAC+AGG	0.424318	2.4:+50111361	None:intergenic
TCTTCGAGGCGAAGAATGTC+GGG	0.429913	2.4:-50110056	MS.gene025139:CDS
TTGTGAAATTCTTTGTCCAC+TGG	0.438129	2.4:+50109747	None:intergenic
AAATGGGAAGAAATTATTGA+TGG	0.442997	2.4:+50109611	None:intergenic
CTTGGGAAAGCGGGAAAACC+AGG	0.448025	2.4:-50113504	MS.gene025139:intron
TGTCCAATCAAAGATGTAAT+CGG	0.448766	2.4:+50111204	None:intergenic
ATTCTAAATCGCTTCTCCAC+CGG	0.454371	2.4:-50113556	MS.gene025139:CDS
TTGTCCACTGGAATGTATGA+TGG	0.458795	2.4:+50109759	None:intergenic
TTATCAAGTGCCAATGTCTT+GGG	0.459266	2.4:-50109889	MS.gene025139:CDS
GTTATCAAGTGCCAATGTCT+TGG	0.467601	2.4:-50109890	MS.gene025139:CDS
TTCTAAATCGCTTCTCCACC+GGG	0.469845	2.4:-50113555	MS.gene025139:CDS
AAGATCGGTAGTGGCTCATT+CGG	0.470396	2.4:-50116805	MS.gene025139:CDS
TTCTGGAATCCCTCCTGCTA+TGG	0.472854	2.4:-50111028	MS.gene025139:CDS
ATATGCAGTCTTCCTTGGCA+AGG	0.473214	2.4:-50111649	MS.gene025139:intron
CCTCATGGGCTTGGGAAAGC+GGG	0.474186	2.4:-50113513	MS.gene025139:CDS
AGTGGATCAAGGCGACTTCA+TGG	0.475207	2.4:-50109859	MS.gene025139:CDS
TCTCATGCTTGCTGAGCAAA+TGG	0.476304	2.4:-50113708	MS.gene025139:intron
GTTACTCTCCTTGGATCTAA+AGG	0.477041	2.4:+50109718	None:intergenic
CGGTCCTAGTGATGCTGCTA+TGG	0.477310	2.4:-50109991	MS.gene025139:intron
GAAGGTTTCTACTTCGATTG+AGG	0.478929	2.4:-50111578	MS.gene025139:intron
ATATTTACAGAATTTAAAAT+GGG	0.479156	2.4:+50110032	None:intergenic
CTCTTCGAGGCGAAGAATGT+CGG	0.480239	2.4:-50110057	MS.gene025139:CDS
ACCTGTAAGGAATGTGTTGA+TGG	0.480755	2.4:+50113345	None:intergenic
AATCTTACAGGAAGGGAAGA+AGG	0.485014	2.4:-50110107	MS.gene025139:intron
CGAGAAAATCAGTGAAAAGA+AGG	0.488049	2.4:-50111596	MS.gene025139:CDS
AGTAGCCGCAATGCATTTGG+CGG	0.491190	2.4:-50109832	MS.gene025139:CDS
TATTAAATGGTTTGGAGTGG+AGG	0.500434	2.4:-50113822	MS.gene025139:CDS
AATTATCTGGTTTAATATCC+CGG	0.500662	2.4:+50113537	None:intergenic
GAATTATGAAACTGCACTCA+AGG	0.502622	2.4:-50109668	MS.gene025139:CDS
CGTGCTACTGAAGATATCAA+TGG	0.508424	2.4:-50109787	MS.gene025139:CDS
ACTAAATCATCATACCTGTA+AGG	0.509141	2.4:+50113332	None:intergenic
TATGCAGTCTTCCTTGGCAA+GGG	0.512407	2.4:-50111648	MS.gene025139:intron
AAACCAGATAATTTCCTCAT+GGG	0.512524	2.4:-50113527	MS.gene025139:CDS
CGAGACCTGTTTATTCGTGA+AGG	0.514655	2.4:-50111366	MS.gene025139:intron
TAAACCAGATAATTTCCTCA+TGG	0.520433	2.4:-50113528	MS.gene025139:CDS
AGAGATGATTTAGAGTCTGT+TGG	0.523766	2.4:-50111793	MS.gene025139:CDS
TCCTCATGGGCTTGGGAAAG+CGG	0.524897	2.4:-50113514	MS.gene025139:CDS
ATGAATACCCACCTTGGCAT+TGG	0.525454	2.4:-50111934	MS.gene025139:intron
ATTAAATGGTTTGGAGTGGA+GGG	0.526860	2.4:-50113821	MS.gene025139:CDS
TGGTTTCAGTAAACTCTTCG+AGG	0.527254	2.4:-50110070	MS.gene025139:CDS
TTGCAGCATTCTGTACAACT+TGG	0.530609	2.4:+50113997	None:intergenic
AGAAGGGCGTCATTCTGGTA+TGG	0.534265	2.4:-50110090	MS.gene025139:CDS
TTTCGTGTTGGTCGTAAGAT+CGG	0.535668	2.4:-50116820	MS.gene025139:CDS
CATAGCAGCATCACTAGGAC+CGG	0.537671	2.4:+50109992	None:intergenic
CTATTAGCATTAGCCATAGC+AGG	0.539572	2.4:+50111015	None:intergenic
AATGGGAAGAAATTATTGAT+GGG	0.543960	2.4:+50109612	None:intergenic
AATCAAAGATGTAATCGGAC+TGG	0.545713	2.4:+50111209	None:intergenic
GTTGGTCGTAAGATCGGTAG+TGG	0.549271	2.4:-50116814	MS.gene025139:CDS
GTTGGGAATAAGTTTCGTGT+TGG	0.550273	2.4:-50116832	MS.gene025139:CDS
AAATATTAAATGGTTTGGAG+TGG	0.550968	2.4:-50113825	MS.gene025139:CDS
TGTCTTGATGTACTTCCTCA+GGG	0.554527	2.4:-50111767	MS.gene025139:CDS
TGGGCGAGTCGAGTGGATCA+AGG	0.557536	2.4:-50109870	MS.gene025139:CDS
ACTCGACTCGCCCAAGACAT+TGG	0.558346	2.4:+50109879	None:intergenic
AGATTACAATGTTCTAGTAA+TGG	0.562002	2.4:-50113798	MS.gene025139:CDS
GTTCTAGTAATGGATCTGCT+TGG	0.563049	2.4:-50113788	MS.gene025139:CDS
CTTACCATAGCAGCATCACT+AGG	0.567272	2.4:+50109987	None:intergenic
ATCTTACAGGAAGGGAAGAA+GGG	0.569393	2.4:-50110106	MS.gene025139:intron
TATCATCAAACCTTAATGAC+CGG	0.570464	2.4:+50111421	None:intergenic
CGTATATACCAATGCCAAGG+TGG	0.570539	2.4:+50111926	None:intergenic
AATGTCTTGGGCGAGTCGAG+TGG	0.571566	2.4:-50109877	MS.gene025139:CDS
ACTGCACTCAAGGGCATCGA+GGG	0.574371	2.4:-50109658	MS.gene025139:CDS
CGTGCTTGAAGCCCTTGCCA+AGG	0.576416	2.4:+50111637	None:intergenic
AAGTATGATGCAAACTCTGT+TGG	0.578562	2.4:+50111453	None:intergenic
TTAAACATCTCTTCAAGACT+AGG	0.585069	2.4:+50113764	None:intergenic
CCACTTACGATAGCACGTGC+TGG	0.587245	2.4:+50111145	None:intergenic
CCCGCTTTCCCAAGCCCATG+AGG	0.590879	2.4:+50113513	None:intergenic
GTATTCCAGGCCTTGTGTCG+AGG	0.592620	2.4:-50111480	MS.gene025139:intron
GGACAAAGAATTTCACAAAG+TGG	0.594554	2.4:-50109742	MS.gene025139:CDS
AAAGTGGACCTTTAGATCCA+AGG	0.597314	2.4:-50109726	MS.gene025139:CDS
AATTATGAAACTGCACTCAA+GGG	0.603669	2.4:-50109667	MS.gene025139:CDS
TGATGTACTTCCTCAGGGGA+AGG	0.612571	2.4:-50111762	MS.gene025139:intron
TGTTGGATAACCTCGACACA+AGG	0.638443	2.4:+50111470	None:intergenic
TTAGCATTAGCCATAGCAGG+AGG	0.649368	2.4:+50111018	None:intergenic
GTATATACCAATGCCAAGGT+GGG	0.649447	2.4:+50111927	None:intergenic
GCTAGCATGAATACCCACCT+TGG	0.649536	2.4:-50111940	MS.gene025139:CDS
AACTGCACTCAAGGGCATCG+AGG	0.650556	2.4:-50109659	MS.gene025139:CDS
GATCGTATATACCAATGCCA+AGG	0.650591	2.4:+50111923	None:intergenic
TAGCATTAGCCATAGCAGGA+GGG	0.651919	2.4:+50111019	None:intergenic
GCTAATGCTAATAGACACAC+AGG	0.661257	2.4:-50111006	MS.gene025139:intron
CAGCACCGCCAAATGCATTG+CGG	0.663018	2.4:+50109827	None:intergenic
TTGGACTCATACAGCAACTG+AGG	0.668956	2.4:+50114016	None:intergenic
CCTGTAAGGAATGTGTTGAT+GGG	0.670053	2.4:+50113346	None:intergenic
GTCTTGATGTACTTCCTCAG+GGG	0.674830	2.4:-50111766	MS.gene025139:CDS
CTTACGATAGCACGTGCTGG+AGG	0.677704	2.4:+50111148	None:intergenic
AAAGCCATCATACATTCCAG+TGG	0.679705	2.4:-50109763	MS.gene025139:CDS
ATAGCAGCATCACTAGGACC+GGG	0.681805	2.4:+50109993	None:intergenic
AAGTTGTACAGAATGCTGCA+AGG	0.682633	2.4:-50113995	MS.gene025139:CDS
GTAATAATCACCTTCCCCTG+AGG	0.710232	2.4:+50111752	None:intergenic
TATCTGGTTTAATATCCCGG+TGG	0.734033	2.4:+50113540	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	50109624	50116866	50109624	ID=MS.gene025139
chr2.4	mRNA	50109624	50116866	50109624	ID=MS.gene025139.t1;Parent=MS.gene025139
chr2.4	exon	50116791	50116866	50116791	ID=MS.gene025139.t1.exon1;Parent=MS.gene025139.t1
chr2.4	CDS	50116791	50116866	50116791	ID=cds.MS.gene025139.t1;Parent=MS.gene025139.t1
chr2.4	exon	50116643	50116683	50116643	ID=MS.gene025139.t1.exon2;Parent=MS.gene025139.t1
chr2.4	CDS	50116643	50116683	50116643	ID=cds.MS.gene025139.t1;Parent=MS.gene025139.t1
chr2.4	exon	50113990	50114059	50113990	ID=MS.gene025139.t1.exon3;Parent=MS.gene025139.t1
chr2.4	CDS	50113990	50114059	50113990	ID=cds.MS.gene025139.t1;Parent=MS.gene025139.t1
chr2.4	exon	50113709	50113857	50113709	ID=MS.gene025139.t1.exon4;Parent=MS.gene025139.t1
chr2.4	CDS	50113709	50113857	50113709	ID=cds.MS.gene025139.t1;Parent=MS.gene025139.t1
chr2.4	exon	50113505	50113600	50113505	ID=MS.gene025139.t1.exon5;Parent=MS.gene025139.t1
chr2.4	CDS	50113505	50113600	50113505	ID=cds.MS.gene025139.t1;Parent=MS.gene025139.t1
chr2.4	exon	50113347	50113417	50113347	ID=MS.gene025139.t1.exon6;Parent=MS.gene025139.t1
chr2.4	CDS	50113347	50113417	50113347	ID=cds.MS.gene025139.t1;Parent=MS.gene025139.t1
chr2.4	exon	50111935	50111996	50111935	ID=MS.gene025139.t1.exon7;Parent=MS.gene025139.t1
chr2.4	CDS	50111935	50111996	50111935	ID=cds.MS.gene025139.t1;Parent=MS.gene025139.t1
chr2.4	exon	50111763	50111826	50111763	ID=MS.gene025139.t1.exon8;Parent=MS.gene025139.t1
chr2.4	CDS	50111763	50111826	50111763	ID=cds.MS.gene025139.t1;Parent=MS.gene025139.t1
chr2.4	exon	50111579	50111663	50111579	ID=MS.gene025139.t1.exon9;Parent=MS.gene025139.t1
chr2.4	CDS	50111579	50111663	50111579	ID=cds.MS.gene025139.t1;Parent=MS.gene025139.t1
chr2.4	exon	50111367	50111493	50111367	ID=MS.gene025139.t1.exon10;Parent=MS.gene025139.t1
chr2.4	CDS	50111367	50111493	50111367	ID=cds.MS.gene025139.t1;Parent=MS.gene025139.t1
chr2.4	exon	50111153	50111235	50111153	ID=MS.gene025139.t1.exon11;Parent=MS.gene025139.t1
chr2.4	CDS	50111153	50111235	50111153	ID=cds.MS.gene025139.t1;Parent=MS.gene025139.t1
chr2.4	exon	50111007	50111070	50111007	ID=MS.gene025139.t1.exon12;Parent=MS.gene025139.t1
chr2.4	CDS	50111007	50111070	50111007	ID=cds.MS.gene025139.t1;Parent=MS.gene025139.t1
chr2.4	exon	50109992	50110119	50109992	ID=MS.gene025139.t1.exon13;Parent=MS.gene025139.t1
chr2.4	CDS	50109992	50110119	50109992	ID=cds.MS.gene025139.t1;Parent=MS.gene025139.t1
chr2.4	exon	50109624	50109911	50109624	ID=MS.gene025139.t1.exon14;Parent=MS.gene025139.t1
chr2.4	CDS	50109624	50109911	50109624	ID=cds.MS.gene025139.t1;Parent=MS.gene025139.t1

Gene Sequence

>MS.gene025139

ATGGAACGTCGCGTTGGGAATAAGTTTCGTGTTGGTCGTAAGATCGGTAGTGGCTCATTCGGAGAGATCTATTTAGGTTGCTCTGTTTTTCTCTCTCTTATGATTTTGTTTGGTTTTTTGTTTTTTTTTTTTTATGTTTCTTGTTTTATTGATCATGTTGGTGTTTTTTGTTTTGAATTGCAGGTACTAACATTGTAACTAATGAAGATGTTGCAATTAAACTTGTAAGTGATGTTTATTAATTGTTTTTTGTTGTTGCTTCATTTGTTTGTTGAATTTGAACTATGAAGAAAAGGGGTAATTTGTTAGATTTTTGTATTGGCATTTAGTTGTTTATTGTTTAGCTATGGTTGATGCAAAACAGGGTTTAGGAAAAGTGGTTGATCTAGTGTAAACCAAATTGATGTATATGCAATCAATGTTAGTGTATATATAAGAAGCCTTCTGACACGGCGGAACTATTCTCATCCACGGTGGAGCAAGTTCTCTGGTGAGATTAGAGTAGACTCAATAGGAGAGTCGGTGGAAAAGTGGTGAAAATGTGACTTGTTCTCGTTGTGACGCGAAGCCACCTTTATATAAAGGATCCCGGCTGTAACTGATTCCATGAAACCCACCGTGGAAGGCAGGAGTTCGTTAGAGATCTTATCGACAGTCATGATTGATGGAACCATGCTCCTATGGTATAAGGACCACATGAAGTTTTCTGTCATGCCTCAAGTGGGGCGAGGATTATAGGTTATTGGACCGGGCTGTCTGGAACAGTGGTTAAATCAACGTTGTTAAACAGCGGCGCTATAGCGCTATTGCATAGCGGAATTTGAGCACATTGCTATTGTTCTGTGATTACGCTATTTAGTACAAATTATTGTGAAATAGCGGTGTTATAACAATGTTGTCTAGCGGAATTTGAAATTACCACTATTATTTGTGATCCACAATTGACAACACTGGGTTAAATGTGAAGAGAATATTTGAAATGGGTAGAGGGATTTATGTTTCAAAAAGTTAATGTAAAAGTGAGTTGACCAAAAACTTTGAGTCTAAAAATCACATGTGGAGATAAAAACTACTGATTCTAGCTTCAAGTTAGAATCAATTTTAGAGGCAAAATCAATTCTACCCGAGAACATCGGAACATGACAAAACCAATTTTGTCTCCAGAACTCTATAGCGCCCTGTGTTGTCAAATGGCGGATACCGGTGGCAAATTTTTGGAAAACCAGTATGGCCAATTTGTGAAGGAAATGGTGGTGCTATACATCAAACAGCATGTCGGATTTTTGGCTTTCTGCCATCAGCCATTGGTAGCATTGATAGCGCCGACACTTCTGATTGAAGGCGTGTTTGGTGTCCAAAACAGATCCATGTGTGTGATTCACAAGCAATTTTATTATTTTCTCAAATAATTACCAGTGCCAAGGTGTCGTGTACGGTGCCTGTGTCAGCGCTTCATAGCTCTAGAATGTCATGAGTTTCTATTCTTGCCATCTTTGACCGTATCTTTGCTGAGCTTGTTGCACTTTCTTATCTTTTGGGGAATGTGACAGGGTTATACATATATCTGATCAATATTTTCAAGCATTAAAAAAAAAGGTTGTTTCTTTTAAAAACCCTTTTGTTGTGAGTGAGGACTTTCTGTTTATGCAGTATTAGTAGGTTTGAAATGATTAATGATGTGTTTGTCTGCCATTGTTCAGAATAGAACTAGCACTTTGTGGTATTGTTCTTCTTGTTTCAACATTTCAGAGCTAGTTGAATCTAAGTTCAATTATTTTGACTGTAAAAAAGAAGTGGACCAGTGTAAATTTTATGTGGCATTGAATGGAAATGCTTCTTACTTGTTGCGTTGTTCCTGACACCATACTGACTGAGAAAAACAAGTATTTGTTTGTGCATAATATTTTCGATCCAACAATTAATTAATTATTGTTATTACTTTGATATTCAATTGTTTTCAGACTGTAGAATACATACATAATCAAGTATTACCTCTATGACATGCATGGCTCTAAGCCTCTAACATGTTTCAAAGAGGTCATTCATATGGAAGTGAAATTTTTCCTTTTACTATTTCACCTTAAAATGCTTGTGCGTGTAATATGCAACTGCTTTTTGCAAGATAAAAGAAGAGTTTCAGTTCCCAGATATTTGAAACATACCATGCAAATTACAATTAATCAAGTCTCCTCAGAAAAATATTGGCATATTCCTTTCTATTCAAAATTCTTATATGTTAATCTCAGAGTCTATGGTTGGGTATTGGATTGGAAGAAATGACATGATTATTTAATCCTGTAATTAATAATCTTTCTCCTCCCTTCCTGAACCAAAGAAAATAAACTGTTGTTAACATGTGACCCGAGTTTCTCTAATTTATTCATTATTTAATCACTTAAAGGTTTACCATATGTGTAGAAAGTAGACATGAAAATAACTATTTTTTATAGTTGAAAACGAAGCGGTCGTCAGTTGCCATTAATAGAAGTAACAGAACCAGTTTCTAACAAAATAATGTATGCTTCATTGGAAAATTTGATGATTAGACTGCAATGCTAGTCTTTGAAAAGATCCTTATATGTGAAATGAATCATCTGTCAAATCATATACTTATTGCTCCGCAAAATTATTAAAATTGAGTAACATCTATTCCTGATATATTCTCTACTCTGTACTTGAACTTTGCTGGTAATTTTGGGCATTGTTTTTCTGTAGCTAGTTACTAGATTCTGGATGTCTCTTAGTGCTAGAGATCTGACTTTGTTGTTACTTTTTGCTTCCTGTTCTTTGCTGCTCTTTTTAATCAGGAAAATGTCAGGACAAAGAATCCTCAGTTGCTGTATGAGTCCAAGTTGTACAGAATGCTGCAAGGAGAGCGTAATGGCCGTTTTCATAAGCATATAACTTTTTTTTATTTTTTATTCCACTTTTTGCAACATACCCTGCTTTTGAATAATTGATCTTATTTGTAACTTATAAATAGCTCTAACTCAAGTTTTATCTTGGCAGCTGGGATTCCAAATATTAAATGGTTTGGAGTGGAGGGAGATTACAATGTTCTAGTAATGGATCTGCTTGGACCTAGTCTTGAAGAGATGTTTAACTTCTGTAGTAGAAAGCTCTCGCTAAAGACAGTTCTCATGCTTGCTGAGCAAATGGTAGGTCCTATTTATACAATGATTTTTTTTTACTAAATACTCAGCCTACCATTGAACTAATAGTAAGTTAAAAAAACTTTTATCCTTCTGTTTTTTTGCCTATGCCAGATCAAACGTGTTGAGTTTGTTCATTCTAAATCGCTTCTCCACCGGGATATTAAACCAGATAATTTCCTCATGGGCTTGGGAAAGCGGGAAAACCAGGTGGCTAAGTTGTTTACGTGGCCTTCTAATGTATTGTATATTCATGTTCATATATTAGGCATTTATTATATATTGGTTTATATGTAGGTTTACTGTATTGATTTTGGTTTAGCGAAGAAATATAGAGATAGTTCGACCCATCAACACATTCCTTACAGGTATGATGATTTAGTTGGTTTGTGAATGCCTTAGTAAACTAGGCTATTCCTAATTGTTAGACCTTGATGGCATAGTGGAATTGATATGCTCAATTTGAAATAATTTCGTCCAATATAGCTGTAACATTTTAATTTATCATAATACTTTTATTTTATTTTAATCTTCATATCGAAAAAATCTTAGCAATGTATATACATGAGGGACGGTTTATATGTATGATTTTTTTATTATGTAATTCAAAGTTTGCGTTATAAAACATTTTTTTGTCAGTATTATCGTGAGTAGATTAACCAATCATGCTTTGCTTGTCTATTGTTGTTTTGATCGCCCACTCAATGTCTGTTTTTGAGTAGTATGATGATGCTCTGAATGTCAGTCTATTCATTAGACGATACTTTAGAAGGGTTTGGAGGCTGGCAGTTGCAATGGCTAGAGATTTGAAGGAAGCTTTAAAACCTTTGACAATCTTCACCTTACAAATAGATTTTGTGGGGTTAAGTTAGACACAAAATCTAAGTTGTATAAAATCTCCTCATACAAAGTGATGAATAAGTAGTAGGAGACCTAAATCTTGAATTCAAGCTGGGCGGATTTAAGTGGAGCGATCCTTTAGGAGTTATGTGGAATGTTAAAGCTCCTTTTGACTTATAAAGAAAACTATATTGAAAAAGACTAATGAGGCTTTGAGGTATTGAATTTTGGAGGATAAAGGGATAAAAGAGATAAAGATAAGATTGAAAAATATGAGCATGTTCTGTTGACTGCAAGAAAAGATAAGATTTTGAATGAATGTTTTGGAGAGAAAGTTAGAACATCTATTTGCCACAGTTATCAATAGCGTGGTATAGCAGCGTATAGCTGCCTCCGGTGACTAGTTTTTCAAACGGGAGTTGTTGCTTTTATGCTATTGTATTTACTATCACTAAAAAAAAAATACCATTTTGGATCTTATTGACACAATTTTTGTAAATATCAAATAGAGGTAGTCAAATCGCTAGAGGTAGACCTATAAAAACTATAAGAGATTAAGAAATACCTAGAGATTAATAAGTTGAATAAATATATGATATATGAGAGGACATTATGGCGTTGATTGTGGGATAAGGCCTGATTGTTGTTGTTTTATAGGCCGGTGATATAAGAACTATATCAAAATTAACCATGTGTAGAATGCTTACGGCATTTCTATTTCATCAAAACTTTGTTTTTCAGGACAAATTGTGATTCTTCTCGGTGATATTTCATCCATATTTAGACCCTAATTTTATATAGCCTAGTTTGGCTGCTGTTGTTTTAGTATTAATTTATTGTGAATGTAACTTGTAATGTATTTCATTTTGAAACAACAGGGAAAATAAAAATTTGACTGGAACTGCAAGATATGCTAGCATGAATACCCACCTTGGCATTGGTATATACGATCTCTTGATACTTACTTAGCATTGCTTAATGTGCATCAAAGTGGTATTATCTAACATATTTTTGTTGCTTGATTCTATTTTTCCGATCATTACTATAGAGCAAAGTCGAAGAGATGATTTAGAGTCTGTTGGTTATGTCTTGATGTACTTCCTCAGGGGAAGGTGATTATTACTGTCAACCTTGCATTGTTGTCAACCTTGCATTGTTTTCTTCTAGTGCTTTGTTGAAACCCATTGGATTGATTTTCATTTTATATGCAGTCTTCCTTGGCAAGGGCTTCAAGCACGAACCAAGAGAGAAAAGTACGAGAAAATCAGTGAAAAGAAGGTTTCTACTTCGATTGAGGTGGGTAGGTTTGATATAATTGGCCTTTGAATACGTAATATGCACTATATCTTAAATTGTACCAATGCCTTGTTATGTATTCCAGGCCTTGTGTCGAGGTTATCCAACAGAGTTTGCATCATACTTCCATTATTGCCGGTCATTAAGGTTTGATGATAGGCCAGATTATGCATACCTCAAAAGAATATTTCGAGACCTGTTTATTCGTGAAGGTTTGAAACTATTCTCATAAAAAAACACCTCTTTGGTGTCAATCTTCGAGTTTATTTTATATATGCTTGCAGGATTTGCTCTTCAATCTATTGCTGCAAATTGATTCATTATCATATTGGCATTTGAACAGGGTTCCAGTCCGATTACATCTTTGATTGGACAATCTTGAAGTATCAGCAATCACAGCTAATCACTCCTCCAGCACGTGCTATCGTAAGTGGTTTTCTCTATATTTTTTTCTCGTTTGAGATCTTTTCCATTAGCAATGGGTAGAATTCTTATGCCTGTGTTTTAGGGGCTTAATGCTGGAACTAGTTCTGGAATCCCTCCTGCTATGGCTAATGCTAATAGACACACAGGTAATTTCTTTGTTGTTTCTGTTTCCTTTTTGACATTAAATTTAGTTTGGTCAGTATAATACGTGTGTGGATGCTTTCTTTGGCAAATTTAAGTCATGTTATGTTTAGGTTACCGTGGTTTAAACCTGTCTTTATCTATCTTGCATGCACCAATATTATTTAGCTTTATTAAATTTATTTGGGAAGAATGACTAAGGACTCGTTTTCTTTTCCAATTCCCTCAGTTTTCTTCTACAATTTTTGAAAACAGATCTAGTATATTTCAAAATTTTGAAAATGTCAACATTTTTCTCATTATTTCTGAAAATGCATTATTTTTAAAATGAACATACATTAGTCTTTCATGTCGTTTGATATTGTTATTCACTAAACTATTCTATCATCTTCTTGTTTGATATTTTCAATGAACATAAATTTAAAAAATGAAAACTTAGAATATTATTAGAAAGTAAAAGGGGCTTAATTTTTTGTCTCCTATTTGTTGCTATTGCTCTGATGTTGTCTTTATTTTTCATGGCCATTTACTTTCCATTCTATGTGTTCATTTTCACTTCAACTAATAAACATAAAATAATACAGATCCAAGATTGTACTCAGCATTTTCTTTTACAATTTTCTATAACAGATCTAGCAATTTTAGAAATTTTGAAATGTCAAAACATTGTTTTCATTGTTCTTGAAAATGCTTTCTTCCTAGCTCGTCTTCCACCTTTTCTGTCACAATTGTTCACGATAAGAGTCTTCCATCTCCTTGTTAGCCTATTTAAAATGAATACTGAAAATATTTTTAGAAAGTAAACAGAGTCTTTCTAATAGGTTTTATGTATTTTTTGCCATATTGAGCTTTTGGGCTTTCTGGTTCTACTTTTTTTCCCATAATCTTACAGGAAGGGAAGAAGGGCGTCATTCTGGTATGGTTTCAGTAAACTCTTCGAGGCGAAGAATGTCGGGACCCATTTTAAATTCTGTAAATATTTCAAACCAGAAAAATCCCGGTCCTAGTGATGCTGCTATGGTAAGATCTTTTGCAAATTTCCTTCAGTTTCCTTTTAAAAAATTACAAAATAATTTGATGTGTTGTGTCAAATCATGCAGTTATCAAGTGCCAATGTCTTGGGCGAGTCGAGTGGATCAAGGCGACTTCATGGTAGCAGTAGCCGCAATGCATTTGGCGGTGCTGATTCAAATTTTCGTGCTCGTGCTACTGAAGATATCAATGGAAAAGCCATCATACATTCCAGTGGACAAAGAATTTCACAAAGTGGACCTTTAGATCCAAGGAGAGTAACATCTTCTAGAAATAGTTCTCATATCAAGAATTATGAAACTGCACTCAAGGGCATCGAGGGTTTGAAATTAAAAAATGTCGATTCCCATCAATAA

Protein sequence

>MS.gene025139.t1

MERRVGNKFRVGRKIGSGSFGEIYLGTNIVTNEDVAIKLENVRTKNPQLLYESKLYRMLQGEPGIPNIKWFGVEGDYNVLVMDLLGPSLEEMFNFCSRKLSLKTVLMLAEQMIKRVEFVHSKSLLHRDIKPDNFLMGLGKRENQVYCIDFGLAKKYRDSSTHQHIPYRENKNLTGTARYASMNTHLGIEQSRRDDLESVGYVLMYFLRGSLPWQGLQARTKREKYEKISEKKVSTSIEALCRGYPTEFASYFHYCRSLRFDDRPDYAYLKRIFRDLFIREGFQSDYIFDWTILKYQQSQLITPPARAIGLNAGTSSGIPPAMANANRHTGREEGRHSGMVSVNSSRRRMSGPILNSVNISNQKNPGPSDAAMLSSANVLGESSGSRRLHGSSSRNAFGGADSNFRARATEDINGKAIIHSSGQRISQSGPLDPRRVTSSRNSSHIKNYETALKGIEGLKLKNVDSHQ