AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028301

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028301.t1 MTR_4g096810 100.000 176 0 0 1 176 1 176 4.65e-129 359
MS.gene028301.t1 MTR_1g475140 34.615 156 96 6 22 173 30 183 8.15e-19 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028301.t1 AT5G59180 91.429 175 15 0 1 175 1 175 5.84e-120 336
MS.gene028301.t1 AT4G14660 32.680 153 98 4 23 172 27 177 1.20e-17 76.6
MS.gene028301.t1 AT4G14660 32.680 153 98 4 23 172 27 177 1.20e-17 76.6
MS.gene028301.t1 AT4G14660 32.680 153 98 4 23 172 27 177 1.20e-17 76.6
MS.gene028301.t1 AT3G22900 31.373 153 101 3 19 168 21 172 2.03e-16 73.2
MS.gene028301.t1 AT3G22900 31.373 153 101 3 19 168 21 172 2.03e-16 73.2

Find 44 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGTGAGGCTAAAGATAAT+TGG 0.250831 4.2:-17456289 MS.gene028301:CDS
ACCCAAGAAATCGTCTTTAA+TGG 0.252979 4.2:+17455004 None:intergenic
TTCGACGTCTTTCATTAGTT+TGG 0.291336 4.2:+17457790 None:intergenic
TCTAAGATCTCTCCCTTAAA+TGG 0.305785 4.2:+17457250 None:intergenic
TCAGTTGATTCCGGATGATA+TGG 0.308719 4.2:-17456498 MS.gene028301:intron
ACAACACATTGATACTTAAC+TGG 0.334226 4.2:+17457280 None:intergenic
TTTCTCCTTTCAGTTGATTC+CGG 0.335903 4.2:-17456507 MS.gene028301:intron
AGGTGAATTTAAACTGTTGC+AGG 0.336659 4.2:+17454969 None:intergenic
TACCATTAAAGACGATTTCT+TGG 0.338650 4.2:-17455006 MS.gene028301:CDS
AAGACGTCGAAGGCACTTGC+AGG 0.412801 4.2:-17457778 MS.gene028301:intron
CCATCTGAAGTAGTGTAGTT+TGG 0.431946 4.2:+17456455 None:intergenic
AAGTGAGGCTAAAGATAATT+GGG 0.441196 4.2:-17456288 MS.gene028301:CDS
TATAAATAAACTTACATGGT+TGG 0.447507 4.2:+17457045 None:intergenic
CAACTGCATCCACGCTACTT+TGG 0.456540 4.2:-17457839 MS.gene028301:CDS
ACCATTAAAGACGATTTCTT+GGG 0.460088 4.2:-17455005 MS.gene028301:CDS
GGATTTGTGGTGGCGGTGAC+GGG 0.462372 4.2:-17457358 MS.gene028301:CDS
TGGATTTGTGGTGGCGGTGA+CGG 0.486501 4.2:-17457359 MS.gene028301:CDS
AGTTTGGCATGTCTCCAGTC+TGG 0.489328 4.2:+17456471 None:intergenic
GTCTGGAATTCCATATCATC+CGG 0.502025 4.2:+17456488 None:intergenic
TTGGAAACGAAGATTTGAAC+TGG 0.502780 4.2:+17457064 None:intergenic
AACTGGAAATGTCACAAATC+CGG 0.505554 4.2:+17457297 None:intergenic
GGAATTGAGAACATTGGTAA+AGG 0.511677 4.2:-17457337 MS.gene028301:CDS
GGGATAATTCGTGATGGGAC+CGG 0.514333 4.2:-17457316 MS.gene028301:CDS
GAATTGAGAACATTGGTAAA+GGG 0.515497 4.2:-17457336 MS.gene028301:CDS
CCAAACTACACTACTTCAGA+TGG 0.523620 4.2:-17456455 MS.gene028301:CDS
CAGTGGTCGACATGGATTTG+TGG 0.554130 4.2:-17457371 MS.gene028301:CDS
TGTTGTTACCATGGTGAACA+AGG 0.559537 4.2:-17457224 MS.gene028301:intron
CTACACTACTTCAGATGGAT+CGG 0.562802 4.2:-17456450 MS.gene028301:intron
GGTGAATTTAAACTGTTGCA+GGG 0.565464 4.2:+17454970 None:intergenic
CTTAGAAGCTGTTGTTACCA+TGG 0.567172 4.2:-17457233 MS.gene028301:CDS
CTAACACACAGTGGTCGACA+TGG 0.581383 4.2:-17457379 MS.gene028301:intron
GGTAAAGGGATAATTCGTGA+TGG 0.598133 4.2:-17457322 MS.gene028301:CDS
GTAAAGGGATAATTCGTGAT+GGG 0.604324 4.2:-17457321 MS.gene028301:CDS
GATGATATGGAATTCCAGAC+TGG 0.604450 4.2:-17456485 MS.gene028301:CDS
TGTTTCGCTCCAGCACTATG+TGG 0.610423 4.2:+17457864 None:intergenic
AAAGATAATTGGGACTCGAG+TGG 0.614804 4.2:-17456278 MS.gene028301:CDS
TCGACATGGATTTGTGGTGG+CGG 0.622425 4.2:-17457365 MS.gene028301:CDS
TGGAAACAAGATTATCACGA+AGG 0.623150 4.2:+17457810 None:intergenic
TGCACAAACCTTGTTCACCA+TGG 0.635657 4.2:+17457216 None:intergenic
GTGACGGGAATTGAGAACAT+TGG 0.640814 4.2:-17457343 MS.gene028301:CDS
TTCAGAAAGATAGTGAAGTG+AGG 0.652885 4.2:-17456303 MS.gene028301:CDS
AAACTAATGAAAGACGTCGA+AGG 0.661613 4.2:-17457788 MS.gene028301:CDS
AGGTTACGACCAAAGTAGCG+TGG 0.680189 4.2:+17457830 None:intergenic
TGGTCGACATGGATTTGTGG+TGG 0.690678 4.2:-17457368 MS.gene028301:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAATTATCATAAACTA+AGG + chr4.2:17456795-17456814 None:intergenic 10.0%
!!! TAATTTTATTTTTAAGTTCA+TGG - chr4.2:17456805-17456824 MS.gene028301:intron 10.0%
!! AAGTTTAATTACTACAATTA+TGG + chr4.2:17455121-17455140 None:intergenic 15.0%
!! AGTTTAATTACTACAATTAT+GGG + chr4.2:17455120-17455139 None:intergenic 15.0%
!! TAATTGTAGTAATTAAACTT+AGG - chr4.2:17455121-17455140 MS.gene028301:intron 15.0%
!!! GTTTTATAAATAAACTTACA+TGG + chr4.2:17455811-17455830 None:intergenic 15.0%
!!! TAACTATTTATCATGTTTTA+TGG - chr4.2:17457753-17457772 MS.gene028301:intron 15.0%
!!! TTCATATTTGCTTTTATAAT+TGG + chr4.2:17456861-17456880 None:intergenic 15.0%
!!! TTTCTTTTCAATTTTGTTTT+TGG - chr4.2:17455219-17455238 MS.gene028301:intron 15.0%
!! AACAATAATAACAGTATCTT+TGG + chr4.2:17455853-17455872 None:intergenic 20.0%
!! AGTTAATTAAAACATCTTAC+AGG + chr4.2:17457500-17457519 None:intergenic 20.0%
!! AGTTATATACAAAAGATCAA+AGG + chr4.2:17457738-17457757 None:intergenic 20.0%
!! ATAAAAGATTAACCTATACT+TGG + chr4.2:17457267-17457286 None:intergenic 20.0%
!! ATAATTTAGCATAAAACTGA+TGG + chr4.2:17456664-17456683 None:intergenic 20.0%
!! ATTTGTACATATCATAAAAG+TGG - chr4.2:17457144-17457163 MS.gene028301:intron 20.0%
!! GAAATAAACCTGTAATAATT+TGG - chr4.2:17455394-17455413 MS.gene028301:intron 20.0%
!! TAATTTAGCATAAAACTGAT+GGG + chr4.2:17456663-17456682 None:intergenic 20.0%
!! TATAAATAAACTTACATGGT+TGG + chr4.2:17455807-17455826 None:intergenic 20.0%
!! TATTATAGACATTGTTAGTT+TGG + chr4.2:17455342-17455361 None:intergenic 20.0%
!! TGATTCTCATTAAGAAATAT+TGG + chr4.2:17457588-17457607 None:intergenic 20.0%
!! TTTAAGAGATTCGAATTAAT+AGG - chr4.2:17456687-17456706 MS.gene028301:intron 20.0%
!!! AAATGAGAATCATGATTTTA+AGG + chr4.2:17455888-17455907 None:intergenic 20.0%
!!! ATTATAGACATTGTTAGTTT+GGG + chr4.2:17455341-17455360 None:intergenic 20.0%
!!! GTTAATCTTTTATCACTTTT+GGG - chr4.2:17457274-17457293 MS.gene028301:CDS 20.0%
!!! TTAATCTTTTATCACTTTTG+GGG - chr4.2:17457275-17457294 MS.gene028301:CDS 20.0%
!!! TTTATTTTTAAGTTCATGGT+AGG - chr4.2:17456809-17456828 MS.gene028301:intron 20.0%
! AAGAAAGAATAACTTAAGAC+AGG + chr4.2:17455944-17455963 None:intergenic 25.0%
! ACAGTGCATTATTTCATTAA+AGG + chr4.2:17457063-17457082 None:intergenic 25.0%
! ACCATTATCTAATGCTAAAA+CGG + chr4.2:17457553-17457572 None:intergenic 25.0%
! AGTTTGATCCAAATTATTAC+AGG + chr4.2:17455405-17455424 None:intergenic 25.0%
! ATAATAATTTCTCGTTGAAG+AGG - chr4.2:17456434-17456453 MS.gene028301:intron 25.0%
! ATTTATGTACTTGAGATTAG+AGG - chr4.2:17455904-17455923 MS.gene028301:intron 25.0%
!! AATGTGTTTTCTTCTGTTTT+TGG - chr4.2:17456491-17456510 MS.gene028301:CDS 25.0%
!! GGTTAATCTTTTATCACTTT+TGG - chr4.2:17457273-17457292 MS.gene028301:CDS 25.0%
!! TAATAAACCTTTTTGTGTCT+TGG - chr4.2:17455357-17455376 MS.gene028301:intron 25.0%
!! TTATAGACATTGTTAGTTTG+GGG + chr4.2:17455340-17455359 None:intergenic 25.0%
!!! AGAGAGCAATTTGTTTTTTT+AGG - chr4.2:17456458-17456477 MS.gene028301:CDS 25.0%
AAGTGAGGCTAAAGATAATT+GGG - chr4.2:17456561-17456580 MS.gene028301:intron 30.0%
AATGAAAAAACCTCAAGTGA+TGG + chr4.2:17455157-17455176 None:intergenic 30.0%
AATTAGTCCAAGACACAAAA+AGG + chr4.2:17455367-17455386 None:intergenic 30.0%
ACAACACATTGATACTTAAC+TGG + chr4.2:17455572-17455591 None:intergenic 30.0%
ACATGAGGAAATAGTAACAA+AGG + chr4.2:17456297-17456316 None:intergenic 30.0%
ACCATTAAAGACGATTTCTT+GGG - chr4.2:17457844-17457863 MS.gene028301:CDS 30.0%
ACTGAAAGCTGCAATATATA+TGG + chr4.2:17456617-17456636 None:intergenic 30.0%
ACTTATGTTCTTCTGAATGA+TGG - chr4.2:17456043-17456062 MS.gene028301:intron 30.0%
AGAAGTAACATCAACAAAGT+AGG + chr4.2:17457446-17457465 None:intergenic 30.0%
AGTAAAGTAAAATTGCAGCA+TGG + chr4.2:17457329-17457348 None:intergenic 30.0%
ATCTTTGGTATAAGTGCAAA+AGG + chr4.2:17455838-17455857 None:intergenic 30.0%
ATTGGAGGAAATAGTAACAA+AGG + chr4.2:17456244-17456263 None:intergenic 30.0%
CAACTGAAAGGAGAAAAAAA+GGG + chr4.2:17456338-17456357 None:intergenic 30.0%
CAAGAAACAAAAGCTATATC+TGG + chr4.2:17456736-17456755 None:intergenic 30.0%
CTAGAGATAGTGTATGAAAA+TGG - chr4.2:17456122-17456141 MS.gene028301:intron 30.0%
CTGAAAGCTGCAATATATAT+GGG + chr4.2:17456616-17456635 None:intergenic 30.0%
GAATTGAGAACATTGGTAAA+GGG - chr4.2:17455513-17455532 MS.gene028301:intron 30.0%
GATCAAGACAAAAATACATG+AGG + chr4.2:17456312-17456331 None:intergenic 30.0%
GTGATAAAAGATTAACCAAG+TGG + chr4.2:17457194-17457213 None:intergenic 30.0%
TACCATTAAAGACGATTTCT+TGG - chr4.2:17457843-17457862 MS.gene028301:CDS 30.0%
TCAACTGAAAGGAGAAAAAA+AGG + chr4.2:17456339-17456358 None:intergenic 30.0%
TTCTTGCAAGGTTTGAAAAA+AGG - chr4.2:17456156-17456175 MS.gene028301:intron 30.0%
! TGTCTTGGACTAATTTTGTT+TGG - chr4.2:17455372-17455391 MS.gene028301:intron 30.0%
! TTTTGAACGTTACTTGAAGA+TGG - chr4.2:17455278-17455297 MS.gene028301:intron 30.0%
!! ACCGTTTTAGCATTAGATAA+TGG - chr4.2:17457549-17457568 MS.gene028301:intron 30.0%
!! ATATAGCTTTTGTTTCTTGC+AGG - chr4.2:17456737-17456756 MS.gene028301:intron 30.0%
!! ATTGTGATATTGAGTTGGAT+AGG - chr4.2:17456962-17456981 MS.gene028301:intron 30.0%
!! GTTGTTTTCAGACCATTTAA+GGG - chr4.2:17455587-17455606 MS.gene028301:intron 30.0%
!! TTTGTGTTGATGCAACTTTT+GGG - chr4.2:17455440-17455459 MS.gene028301:intron 30.0%
!!! GTTTTCATTGATTTCTTGCA+AGG - chr4.2:17456144-17456163 MS.gene028301:intron 30.0%
!!! TGATCGTTTGTTTTTTTTGC+AGG - chr4.2:17456517-17456536 MS.gene028301:intron 30.0%
!!! TGTTGTTTTCAGACCATTTA+AGG - chr4.2:17455586-17455605 MS.gene028301:intron 30.0%
AAACTAATGAAAGACGTCGA+AGG - chr4.2:17455061-17455080 MS.gene028301:intron 35.0%
AACTGGAAATGTCACAAATC+CGG + chr4.2:17455555-17455574 None:intergenic 35.0%
AAGGCAAAACAGAATAGCTA+CGG + chr4.2:17456225-17456244 None:intergenic 35.0%
ACCCAAGAAATCGTCTTTAA+TGG + chr4.2:17457848-17457867 None:intergenic 35.0%
AGGAGAATAATAACCCTTCT+CGG + chr4.2:17457426-17457445 None:intergenic 35.0%
AGTGAGGGGAATGAAAAAAT+AGG + chr4.2:17457635-17457654 None:intergenic 35.0%
ATAAACTAAGGCTGTTCTCT+AGG + chr4.2:17456783-17456802 None:intergenic 35.0%
ATATGAATAGACCCACTACA+TGG - chr4.2:17456874-17456893 MS.gene028301:intron 35.0%
CGAATACAATGAACACTGAT+CGG - chr4.2:17457674-17457693 MS.gene028301:intron 35.0%
CTAATGCTAAAACGGTAACT+AGG + chr4.2:17457545-17457564 None:intergenic 35.0%
CTGTATTAAGATGCAGAACA+TGG - chr4.2:17457350-17457369 MS.gene028301:CDS 35.0%
GAAGTGAGGCTAAAGATAAT+TGG - chr4.2:17456560-17456579 MS.gene028301:intron 35.0%
GGAATTGAGAACATTGGTAA+AGG - chr4.2:17455512-17455531 MS.gene028301:intron 35.0%
GTAAAGGGATAATTCGTGAT+GGG - chr4.2:17455528-17455547 MS.gene028301:intron 35.0%
TATCAGTTCATTGCATTCAC+AGG - chr4.2:17457108-17457127 MS.gene028301:intron 35.0%
TATTCGTGTTATAGCCCTAA+GGG + chr4.2:17457660-17457679 None:intergenic 35.0%
TCAGGATTGTGATATTGAGT+TGG - chr4.2:17456957-17456976 MS.gene028301:intron 35.0%
TCTTACAGGATAATCATGCA+AGG + chr4.2:17457486-17457505 None:intergenic 35.0%
TGAAAGTGACTACCAAGTAT+AGG - chr4.2:17457252-17457271 MS.gene028301:CDS 35.0%
TGAGTTCTGTTTGTACAGAT+GGG - chr4.2:17455743-17455762 MS.gene028301:intron 35.0%
TGGAAACAAGATTATCACGA+AGG + chr4.2:17455042-17455061 None:intergenic 35.0%
TTCAGAAAGATAGTGAAGTG+AGG - chr4.2:17456546-17456565 MS.gene028301:intron 35.0%
TTCGACGTCTTTCATTAGTT+TGG + chr4.2:17455062-17455081 None:intergenic 35.0%
TTGAGTTCTGTTTGTACAGA+TGG - chr4.2:17455742-17455761 MS.gene028301:intron 35.0%
TTGGAAACGAAGATTTGAAC+TGG + chr4.2:17455788-17455807 None:intergenic 35.0%
! TATGCTAGTGTTTTACCACT+TGG - chr4.2:17457176-17457195 MS.gene028301:intron 35.0%
! TCTAAGATCTCTCCCTTAAA+TGG + chr4.2:17455602-17455621 None:intergenic 35.0%
! TTGTTAGTTTGGGGTTTAAG+AGG + chr4.2:17455331-17455350 None:intergenic 35.0%
! TTTCCTCATCAGCTTTATCT+AGG - chr4.2:17455674-17455693 MS.gene028301:intron 35.0%
! TTTCTCCTTTCAGTTGATTC+CGG - chr4.2:17456342-17456361 MS.gene028301:intron 35.0%
!! AATAGGATATGAAAGCACTG+TGG - chr4.2:17456704-17456723 MS.gene028301:intron 35.0%
!! AGATCTAGCTCTACAATGAA+AGG + chr4.2:17457035-17457054 None:intergenic 35.0%
!! CTTTGTGTTGATGCAACTTT+TGG - chr4.2:17455439-17455458 MS.gene028301:intron 35.0%
!!! GGATCAAACTGTTTCTGTTT+TGG - chr4.2:17455415-17455434 MS.gene028301:intron 35.0%
AAAGATAATTGGGACTCGAG+TGG - chr4.2:17456571-17456590 MS.gene028301:intron 40.0%
ACAAAAGATGCCATCACTTG+AGG - chr4.2:17455144-17455163 MS.gene028301:intron 40.0%
ACACCTAGATAAAGCTGATG+AGG + chr4.2:17455680-17455699 None:intergenic 40.0%
AGGCAAAACAGAATAGCTAC+GGG + chr4.2:17456224-17456243 None:intergenic 40.0%
ATCATCCGGAATCAACTGAA+AGG + chr4.2:17456350-17456369 None:intergenic 40.0%
ATGGTAGGTATACACGAGAA+GGG - chr4.2:17456893-17456912 MS.gene028301:intron 40.0%
ATGTTGATCCCTGTTGACAT+GGG + chr4.2:17457228-17457247 None:intergenic 40.0%
ATTGGATCACCTGTGTCATA+AGG + chr4.2:17456843-17456862 None:intergenic 40.0%
CAACTTGACTGCAACAAGTT+TGG + chr4.2:17456016-17456035 None:intergenic 40.0%
CCAAACTACACTACTTCAGA+TGG - chr4.2:17456394-17456413 MS.gene028301:intron 40.0%
CTACACTACTTCAGATGGAT+CGG - chr4.2:17456399-17456418 MS.gene028301:intron 40.0%
CTACGGGAGAGACTAAAATT+GGG + chr4.2:17456208-17456227 None:intergenic 40.0%
CTTAGAAGCTGTTGTTACCA+TGG - chr4.2:17455616-17455635 MS.gene028301:intron 40.0%
GAGAGAATATCTCTCTGGTT+AGG - chr4.2:17456984-17457003 MS.gene028301:intron 40.0%
GATAGGAGAGAATATCTCTC+TGG - chr4.2:17456979-17456998 MS.gene028301:intron 40.0%
GATGATATGGAATTCCAGAC+TGG - chr4.2:17456364-17456383 MS.gene028301:intron 40.0%
GGTAAAGGGATAATTCGTGA+TGG - chr4.2:17455527-17455546 MS.gene028301:intron 40.0%
GTATTCGTGTTATAGCCCTA+AGG + chr4.2:17457661-17457680 None:intergenic 40.0%
GTGTATACCTACCATGTAGT+GGG + chr4.2:17456888-17456907 None:intergenic 40.0%
GTTGCATTGCTCATAACTGT+CGG - chr4.2:17457081-17457100 MS.gene028301:CDS 40.0%
TAAGTTCATGGTAGGACCTT+GGG - chr4.2:17456817-17456836 MS.gene028301:intron 40.0%
TGAGGAAAATGTAGAGACAG+AGG + chr4.2:17455662-17455681 None:intergenic 40.0%
TGTGTCGAATGTCGATAAAG+TGG - chr4.2:17455710-17455729 MS.gene028301:intron 40.0%
TGTTGTTACCATGGTGAACA+AGG - chr4.2:17455625-17455644 MS.gene028301:intron 40.0%
TTAAGTTCATGGTAGGACCT+TGG - chr4.2:17456816-17456835 MS.gene028301:intron 40.0%
! AGTTACTTTATGCACCGAGA+AGG - chr4.2:17457409-17457428 MS.gene028301:intron 40.0%
! CCATCTGAAGTAGTGTAGTT+TGG + chr4.2:17456397-17456416 None:intergenic 40.0%
! GTCTGGAATTCCATATCATC+CGG + chr4.2:17456364-17456383 None:intergenic 40.0%
! GTTACTTTATGCACCGAGAA+GGG - chr4.2:17457410-17457429 MS.gene028301:intron 40.0%
! TCAGTTGATTCCGGATGATA+TGG - chr4.2:17456351-17456370 MS.gene028301:intron 40.0%
! TTCTCGGTGCATAAAGTAAC+TGG + chr4.2:17457410-17457429 None:intergenic 40.0%
!!! ATGGGTTTTTTTGCTGAAGC+TGG - chr4.2:17455761-17455780 MS.gene028301:intron 40.0%
AAAACGGTAACTAGGCTGCT+AGG + chr4.2:17457537-17457556 None:intergenic 45.0%
ACGGGAGAGACTAAAATTGG+AGG + chr4.2:17456206-17456225 None:intergenic 45.0%
CATGGTAGGTATACACGAGA+AGG - chr4.2:17456892-17456911 MS.gene028301:intron 45.0%
CATGTCAACAGGGATCAACA+TGG - chr4.2:17457227-17457246 MS.gene028301:CDS 45.0%
CATGTTGATCCCTGTTGACA+TGG + chr4.2:17457229-17457248 None:intergenic 45.0%
CCTATGCAGAACTGCAAAAG+AGG + chr4.2:17457823-17457842 None:intergenic 45.0%
CGTGTATACCTACCATGTAG+TGG + chr4.2:17456889-17456908 None:intergenic 45.0%
GAATAGACCCACTACATGGT+AGG - chr4.2:17456878-17456897 MS.gene028301:intron 45.0%
GCTACGGGAGAGACTAAAAT+TGG + chr4.2:17456209-17456228 None:intergenic 45.0%
GGCTTTCAACTAACACACAG+TGG - chr4.2:17455461-17455480 MS.gene028301:intron 45.0%
GGGTTTAAGAGGTCTGTTAC+AGG + chr4.2:17455320-17455339 None:intergenic 45.0%
TCCAACAACCCATGTCAACA+GGG - chr4.2:17457217-17457236 MS.gene028301:intron 45.0%
TGCACAAACCTTGTTCACCA+TGG + chr4.2:17455636-17455655 None:intergenic 45.0%
TGGTAGGTATACACGAGAAG+GGG - chr4.2:17456894-17456913 MS.gene028301:intron 45.0%
TTCCAACAACCCATGTCAAC+AGG - chr4.2:17457216-17457235 MS.gene028301:intron 45.0%
! CCTCTTTTGCAGTTCTGCAT+AGG - chr4.2:17457820-17457839 MS.gene028301:CDS 45.0%
! GTGACGGGAATTGAGAACAT+TGG - chr4.2:17455506-17455525 MS.gene028301:intron 45.0%
! GTTGACATGGGTTGTTGGAA+TGG + chr4.2:17457216-17457235 None:intergenic 45.0%
AGGTTACGACCAAAGTAGCG+TGG + chr4.2:17455022-17455041 None:intergenic 50.0%
AGTTTGGCATGTCTCCAGTC+TGG + chr4.2:17456381-17456400 None:intergenic 50.0%
ATACACGAGAAGGGGAAGAC+TGG - chr4.2:17456902-17456921 MS.gene028301:intron 50.0%
CAACTGCATCCACGCTACTT+TGG - chr4.2:17455010-17455029 MS.gene028301:CDS 50.0%
CAGTGGTCGACATGGATTTG+TGG - chr4.2:17455478-17455497 MS.gene028301:intron 50.0%
CTAACACACAGTGGTCGACA+TGG - chr4.2:17455470-17455489 MS.gene028301:intron 50.0%
TCGACATGGATTTGTGGTGG+CGG - chr4.2:17455484-17455503 MS.gene028301:intron 50.0%
TGGTCGACATGGATTTGTGG+TGG - chr4.2:17455481-17455500 MS.gene028301:intron 50.0%
TGTTTCGCTCCAGCACTATG+TGG + chr4.2:17454988-17455007 None:intergenic 50.0%
! AAGGGGAAGACTGGTGTTAG+TGG - chr4.2:17456911-17456930 MS.gene028301:intron 50.0%
! AGGGGAAGACTGGTGTTAGT+GGG - chr4.2:17456912-17456931 MS.gene028301:intron 50.0%
! GGGATAATTCGTGATGGGAC+CGG - chr4.2:17455533-17455552 MS.gene028301:intron 50.0%
! TCCCTGTTGACATGGGTTGT+TGG + chr4.2:17457221-17457240 None:intergenic 50.0%
!! GTCTAGGTGTGAGGGTATTC+AGG - chr4.2:17456939-17456958 MS.gene028301:intron 50.0%
!! TGGTTAGGAGTCACTGACTC+TGG - chr4.2:17456999-17457018 MS.gene028301:intron 50.0%
ATAGCCCTAAGGGCGAGTGA+GGG + chr4.2:17457650-17457669 None:intergenic 55.0%
ATTCCCCTCACTCGCCCTTA+GGG - chr4.2:17457643-17457662 MS.gene028301:intron 55.0%
CACCTGTGTCATAAGGCCCA+AGG + chr4.2:17456836-17456855 None:intergenic 55.0%
GGTGTTAGTGGGGAGAGTCT+AGG - chr4.2:17456923-17456942 MS.gene028301:intron 55.0%
TATAGCCCTAAGGGCGAGTG+AGG + chr4.2:17457651-17457670 None:intergenic 55.0%
TGGGGAGAGTCTAGGTGTGA+GGG - chr4.2:17456931-17456950 MS.gene028301:intron 55.0%
! GACCTTGGGCCTTATGACAC+AGG - chr4.2:17456831-17456850 MS.gene028301:intron 55.0%
! GGGGAAGACTGGTGTTAGTG+GGG - chr4.2:17456913-17456932 MS.gene028301:intron 55.0%
!! AAGACGTCGAAGGCACTTGC+AGG - chr4.2:17455071-17455090 MS.gene028301:intron 55.0%
!! TGGATTTGTGGTGGCGGTGA+CGG - chr4.2:17455490-17455509 MS.gene028301:intron 55.0%
CATTCCCCTCACTCGCCCTT+AGG - chr4.2:17457642-17457661 MS.gene028301:intron 60.0%
GTGGGGAGAGTCTAGGTGTG+AGG - chr4.2:17456930-17456949 MS.gene028301:intron 60.0%
TAGCCCTAAGGGCGAGTGAG+GGG + chr4.2:17457649-17457668 None:intergenic 60.0%
!! GGATTTGTGGTGGCGGTGAC+GGG - chr4.2:17455491-17455510 MS.gene028301:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 17454977 17457894 17454977 ID=MS.gene028301
chr4.2 mRNA 17454977 17457894 17454977 ID=MS.gene028301.t1;Parent=MS.gene028301
chr4.2 exon 17457779 17457894 17457779 ID=MS.gene028301.t1.exon1;Parent=MS.gene028301.t1
chr4.2 CDS 17457779 17457894 17457779 ID=cds.MS.gene028301.t1;Parent=MS.gene028301.t1
chr4.2 exon 17457225 17457390 17457225 ID=MS.gene028301.t1.exon2;Parent=MS.gene028301.t1
chr4.2 CDS 17457225 17457390 17457225 ID=cds.MS.gene028301.t1;Parent=MS.gene028301.t1
chr4.2 exon 17457060 17457110 17457060 ID=MS.gene028301.t1.exon3;Parent=MS.gene028301.t1
chr4.2 CDS 17457060 17457110 17457060 ID=cds.MS.gene028301.t1;Parent=MS.gene028301.t1
chr4.2 exon 17456451 17456516 17456451 ID=MS.gene028301.t1.exon4;Parent=MS.gene028301.t1
chr4.2 CDS 17456451 17456516 17456451 ID=cds.MS.gene028301.t1;Parent=MS.gene028301.t1
chr4.2 exon 17456264 17456332 17456264 ID=MS.gene028301.t1.exon5;Parent=MS.gene028301.t1
chr4.2 CDS 17456264 17456332 17456264 ID=cds.MS.gene028301.t1;Parent=MS.gene028301.t1
chr4.2 exon 17454977 17455039 17454977 ID=MS.gene028301.t1.exon6;Parent=MS.gene028301.t1
chr4.2 CDS 17454977 17455039 17454977 ID=cds.MS.gene028301.t1;Parent=MS.gene028301.t1
Gene Sequence

>MS.gene028301

ATGTTTTTCCACATAGTGCTGGAGCGAAACATGCAACTGCATCCACGCTACTTTGGTCGTAACCTTCGTGATAATCTTGTTTCCAAACTAATGAAAGACGTCGAAGGCACTTGCAGGTGATTCATCTTAAGATCATTTCACCCATAATTGTAGTAATTAAACTTAGGACAAAAGATGCCATCACTTGAGGTTTTTTCATTAACAGACTACTACTTGAAGAATACGTACAATGTTGTCATTGATTTCTTTTCAATTTTGTTTTTGGTTATCATTTAAAGAAGACAAATTAAGTAGAACTACATTTTGAACGTTACTTGAAGATGGAAACAGTTTCAATTGACCTGTAACAGACCTCTTAAACCCCAAACTAACAATGTCTATAATAAACCTTTTTGTGTCTTGGACTAATTTTGTTTGGAAATAAACCTGTAATAATTTGGATCAAACTGTTTCTGTTTTGGACTTTGTGTTGATGCAACTTTTGGGCTTTCAACTAACACACAGTGGTCGACATGGATTTGTGGTGGCGGTGACGGGAATTGAGAACATTGGTAAAGGGATAATTCGTGATGGGACCGGATTTGTGACATTTCCAGTTAAGTATCAATGTGTTGTTTTCAGACCATTTAAGGGAGAGATCTTAGAAGCTGTTGTTACCATGGTGAACAAGGTTTGTGCAATTCCTCTGTCTCTACATTTTCCTCATCAGCTTTATCTAGGTGTCTGACTCTGCTGTGTCGAATGTCGATAAAGTGGTTTATATTATTGAGTTCTGTTTGTACAGATGGGTTTTTTTGCTGAAGCTGGACCAGTTCAAATCTTCGTTTCCAACCATGTAAGTTTATTTATAAAACTTCACCTTTTGCACTTATACCAAAGATACTGTTATTATTGTTCTTCATTTTAAGCCTTAAAATCATGATTCTCATTTATGTACTTGAGATTAGAGGAGTAAAAGACATTACCTGTCTTAAGTTATTCTTTCTTATCAAAAAATTAAATGCACATTCTGAAAAAAATAATTACTGAATGCTGTCCAAACTTGTTGCAGTCAAGTTGCATAAGCACTTATGTTCTTCTGAATGATGGAAAAGACATCGACTTCTTGTTTTATCATTTGTCATATTGACTATATAGTATATACACTAGAGATAGTGTATGAAAATGGTTTTCATTGATTTCTTGCAAGGTTTGAAAAAAGGATAATTTGCAAGCTTTTGAATTGTGACCCAATTTTAGTCTCTCCCGTAGCTATTCTGTTTTGCCTTTGTTACTATTTCCTCCAATTTTAGTCTCTCCCGTAGCTATTCTGTTTTGCCTTTGTTACTATTTCCTCATGTATTTTTGTCTTGATCAGACCCTTTTTTTCTCCTTTCAGTTGATTCCGGATGATATGGAATTCCAGACTGGAGACATGCCAAACTACACTACTTCAGATGGATCGGTACATATCATTTATAATAATTTCTCGTTGAAGAGGAAGAGAGCAATTTGTTTTTTTAGGTGTTCAATATAATGTGTTTTCTTCTGTTTTTGGCTTTGATCGTTTGTTTTTTTTGCAGGTTAAGATTCAGAAAGATAGTGAAGTGAGGCTAAAGATAATTGGGACTCGAGTGGATGCTACTGAAATTGTAAGCCCATATATATTGCAGCTTTCAGTTTGCTTTTATTTATGTTGTTAAGCCCATCAGTTTTATGCTAAATTATACTTTTAAGAGATTCGAATTAATAGGATATGAAAGCACTGTGGTCTGTGCCAGATATAGCTTTTGTTTCTTGCAGGTCTGTTTACTAGTACAAATTCCTAGAGAACAGCCTTAGTTTATGATAATTTTATTTTTAAGTTCATGGTAGGACCTTGGGCCTTATGACACAGGTGATCCAATTATAAAAGCAAATATGAATAGACCCACTACATGGTAGGTATACACGAGAAGGGGAAGACTGGTGTTAGTGGGGAGAGTCTAGGTGTGAGGGTATTCAGGATTGTGATATTGAGTTGGATAGGAGAGAATATCTCTCTGGTTAGGAGTCACTGACTCTGGATGTACTTTTCCTTTCATTGTAGAGCTAGATCTCATTTCCTTTAATGAAATAATGCACTGTTGCATTGCTCATAACTGTCGGTTTCTATCAGTTCATTGCATTCACAGGTTTAGCAGTAGTGATTTGTACATATCATAAAAGTGGCGATAAATTTATGCTAGTGTTTTACCACTTGGTTAATCTTTTATCACCATTCCAACAACCCATGTCAACAGGGATCAACATGGAATGAAAGTGACTACCAAGTATAGGTTAATCTTTTATCACTTTTGGGGTTTGCAAGAATCATTATTCATTACTGCTCCATGCTGCAATTTTACTTTACTACTGTATTAAGATGCAGAACATGGTAGAACTTGTGAGCAAATCGTGATCACAAATTGTCCAGTTACTTTATGCACCGAGAAGGGTTATTATTCTCCTACTTTGTTGATGTTACTTCTGATTATTTTTCATATTTCCTTGCATGATTATCCTGTAAGATGTTTTAATTAACTTGTGATAGCGATGACCTAGCAGCCTAGTTACCGTTTTAGCATTAGATAATGGTTTTTTTTTCTTTCCAATATTTCTTAATGAGAATCAAGTTATACTCTTGCACATGCTAAGCCTATTTTTTCATTCCCCTCACTCGCCCTTAGGGCTATAACACGAATACAATGAACACTGATCGGTGCAACTCTTCAGTTTCTCATAGCTATTAAATTTTTTTCCTTTGATCTTTTGTATATAACTATTTATCATGTTTTATGGTTTCAATATCTTCAAGAACTCATTTATTTAATGTCACATATTATCCTCTTTTGCAGTTCTGCATAGGTACCATTAAAGACGATTTCTTGGGTGTGATCAACGACCCTGCAACAGTTTAA
Protein sequence

>MS.gene028301.t1

MFFHIVLERNMQLHPRYFGRNLRDNLVSKLMKDVEGTCSGRHGFVVAVTGIENIGKGIIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQTGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPATV