AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20258

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20258.t1 MTR_5g016220 91.239 331 27 1 1 329 1 331 0.0 616
MS.gene20258.t1 MTR_5g016200 90.547 201 16 1 125 325 2 199 1.46e-131 373
MS.gene20258.t1 MTR_5g016200 79.365 126 22 2 1 124 1 124 6.69e-61 193
MS.gene20258.t1 MTR_5g005470 80.000 205 41 0 125 329 2 206 3.41e-122 349
MS.gene20258.t1 MTR_5g005470 72.441 127 32 2 1 124 1 127 5.31e-56 181
MS.gene20258.t1 MTR_6g042230 61.765 204 77 1 126 329 3 205 2.95e-82 248
MS.gene20258.t1 MTR_6g042230 47.368 133 65 2 1 131 1 130 5.88e-29 110
MS.gene20258.t1 MTR_6g088010 56.311 206 88 1 125 328 2 207 3.62e-80 243
MS.gene20258.t1 MTR_6g088010 45.985 137 64 3 1 131 1 133 2.67e-29 112
MS.gene20258.t1 MTR_6g088020 55.825 206 89 1 125 328 2 207 3.74e-77 235
MS.gene20258.t1 MTR_6g088020 42.105 133 75 1 1 131 1 133 3.38e-24 98.2
MS.gene20258.t1 MTR_7g090220 32.932 249 157 4 88 328 1 247 4.31e-39 139
MS.gene20258.t1 MTR_3g058520 50.769 65 31 1 253 317 95 158 1.32e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20258.t1 AT3G22320 65.854 205 70 0 125 329 1 205 1.55e-100 295
MS.gene20258.t1 AT3G22320 53.968 126 55 2 2 124 1 126 6.43e-38 134
MS.gene20258.t1 AT5G57980 43.204 206 113 2 128 329 5 210 1.02e-55 181
MS.gene20258.t1 AT5G57980 32.283 127 79 4 5 124 5 131 8.27e-16 75.5
MS.gene20258.t1 AT2G41340 40.394 203 118 2 127 328 17 217 1.33e-44 152
MS.gene20258.t1 AT3G57080 35.023 217 138 2 113 328 7 221 6.98e-40 140
MS.gene20258.t1 AT3G54490 32.338 201 133 2 130 329 35 233 1.35e-30 116
MS.gene20258.t1 AT2G41340 36.313 179 102 3 127 304 17 184 1.40e-28 109
MS.gene20258.t1 AT2G41340 38.650 163 97 2 127 288 17 177 1.87e-28 108
MS.gene20258.t1 AT3G16680 44.156 77 41 1 207 281 2 78 3.45e-14 67.8

Find 82 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTTGGCGTCCAGACTTTC+AGG 0.249538 5.1:-8523450 MS.gene20258:CDS
ACATACCGATTTGTTGTCTA+AGG 0.261902 5.1:-8522332 None:intergenic
CAAGAGTTCAAGGAGAAATA+TGG 0.263586 5.1:-8523565 MS.gene20258:CDS
GGATCCCTCACCTGGATTCT+AGG 0.310631 5.1:+8522434 None:intergenic
GATCCTGTTGCAAGATATTA+TGG 0.316385 5.1:-8522416 MS.gene20258:CDS
CCAGATTTATGTCTTCTTCC+CGG 0.347022 5.1:-8523482 MS.gene20258:CDS
TCAGTCAGCACCTGATGTTC+TGG 0.354438 5.1:+8523164 None:intergenic
CTTGCAGGAGGCTGAATTGC+TGG 0.356558 5.1:-8523213 MS.gene20258:intron
ACATGTTAGTAAGGCTGTTC+TGG 0.357577 5.1:+8523374 None:intergenic
ATGAAATAATGAAGCTGTAT+AGG 0.373700 5.1:-8524032 MS.gene20258:CDS
AATCGCCTCCAAATTTCATT+TGG 0.374113 5.1:-8523710 MS.gene20258:CDS
GTTTGCCTCCAAATTTCATT+TGG 0.384993 5.1:-8523332 MS.gene20258:CDS
ACGTTTGGTGTAAGTCCTTA+AGG 0.390806 5.1:+8523810 None:intergenic
ACTGACCTGAGTTTCTTTAA+CGG 0.397368 5.1:+8523109 None:intergenic
GCATCTCCATCACTGTCTTC+CGG 0.401574 5.1:+8523638 None:intergenic
AAGAGTTCAAGGAGAAATAT+GGG 0.406548 5.1:-8523564 MS.gene20258:CDS
CAAGCTTGTGCCAGAACATC+AGG 0.413166 5.1:-8523174 MS.gene20258:CDS
CATGTCCAAGCAAGAGTTCA+AGG 0.415521 5.1:-8523575 MS.gene20258:CDS
CCGCTTTAGTAAGGCTGGTC+TGG 0.426050 5.1:+8523749 None:intergenic
TAATGAAGCTGTATAGGATC+TGG 0.428962 5.1:-8524026 MS.gene20258:CDS
ATGCCATAATATCTTGCAAC+AGG 0.434750 5.1:+8522413 None:intergenic
ATGAAATATCGAAGCTGTAT+AGG 0.436885 5.1:-8523663 MS.gene20258:CDS
TTTCTCCTTGAACTCTTGCT+TGG 0.437286 5.1:+8523570 None:intergenic
CGCCAACCTTGTCATTCAAC+GGG 0.438451 5.1:+8523839 None:intergenic
AGGTTGGCGTCCACACCTTA+AGG 0.442532 5.1:-8523825 MS.gene20258:CDS
TTGCAACAGGATCCCTCACC+TGG 0.449627 5.1:+8522426 None:intergenic
ACGCCAACCTTGTCATCCTC+CGG 0.452689 5.1:+8523463 None:intergenic
TGGTGTAAGTCCTGAAAGTC+TGG 0.458394 5.1:+8523440 None:intergenic
ATTCTCGGAGTTCATACGTT+TGG 0.464348 5.1:+8523420 None:intergenic
GACAAGTTGTCAAGATAATC+CGG 0.473708 5.1:-8522382 MS.gene20258:CDS
CAGCCTTACTAAAGCGGCAC+TGG 0.478297 5.1:-8523743 MS.gene20258:CDS
AGTCCAGTGCCGCTTTAGTA+AGG 0.480596 5.1:+8523740 None:intergenic
ACGGGAAGAACACATAAATC+TGG 0.480965 5.1:+8523857 None:intergenic
GATGGCTCTGTAGACATTCT+CGG 0.488016 5.1:+8523405 None:intergenic
GATGGCTCTGTAGACATTCT+CGG 0.488016 5.1:+8523780 None:intergenic
TTCCTGCAGTCTCACTTGGC+CGG 0.488931 5.1:+8522363 None:intergenic
AACACATAAATCTGGTCAAA+GGG 0.490918 5.1:+8523865 None:intergenic
TATCGAAGCTGTATAGGATC+CGG 0.503346 5.1:-8523657 MS.gene20258:CDS
GCTGTTCTGGCAAACGATGA+TGG 0.506683 5.1:+8523387 None:intergenic
GCTGGTCTGGCAAACGATGA+TGG 0.506683 5.1:+8523762 None:intergenic
ATTCTCGGAGTCCATACGTT+TGG 0.507587 5.1:+8523795 None:intergenic
CCGGGAAGAAGACATAAATC+TGG 0.508593 5.1:+8523482 None:intergenic
TGTTCCATGACATGTTAGTA+AGG 0.511336 5.1:+8523365 None:intergenic
AACTTCCAAATGAAATTTGG+AGG 0.512170 5.1:+8523327 None:intergenic
AACTTCCAAATGAAATTTGG+AGG 0.512170 5.1:+8523705 None:intergenic
TAGGATCTGGAAGAAGGTGA+TGG 0.515671 5.1:-8524013 MS.gene20258:CDS
GGTTAACATACAAGACTTCA+AGG 0.520090 5.1:-8523950 MS.gene20258:CDS
TGATGGAGATGCTTCGTGAC+AGG 0.522662 5.1:-8523627 MS.gene20258:CDS
TGATGGAGATGCTTCGCGAC+AGG 0.523509 5.1:-8523996 MS.gene20258:CDS
TATCTTCCTGCAGTCTCACT+TGG 0.525204 5.1:+8522359 None:intergenic
CGCCAACCTTGTCATCCTCC+GGG 0.525327 5.1:+8523464 None:intergenic
ATCTCAGCTGCCTAGAATCC+AGG 0.526579 5.1:-8522444 MS.gene20258:intron
GAAATCCTTAGACAACAAAT+CGG 0.526850 5.1:+8522327 None:intergenic
CAGTGCCGCTTTAGTAAGGC+TGG 0.528736 5.1:+8523744 None:intergenic
TTCCCGGAGGATGACAAGGT+TGG 0.531846 5.1:-8523466 MS.gene20258:CDS
TTACCTCGTAGAAGATTTCG+AGG 0.547378 5.1:-8523971 MS.gene20258:CDS
GCTGTATAGGATCTGGAAGA+AGG 0.551009 5.1:-8524019 MS.gene20258:CDS
CCAGACCAGCCTTACTAAAG+CGG 0.552518 5.1:-8523749 MS.gene20258:CDS
GAACACATAAATCTGGTCAA+AGG 0.555684 5.1:+8523864 None:intergenic
ACGCCAACCTTGTCATTCAA+CGG 0.558382 5.1:+8523838 None:intergenic
TTCCCGTTGAATGACAAGGT+TGG 0.562118 5.1:-8523841 MS.gene20258:CDS
AACGGTGTATCTCTCAAGTA+CGG 0.566449 5.1:+8523127 None:intergenic
CTTCTTCCCGGAGGATGACA+AGG 0.570040 5.1:-8523470 MS.gene20258:CDS
AGATATTATGGCATGAAGCG+TGG 0.584475 5.1:-8522404 MS.gene20258:CDS
ACAGCCTTACTAACATGTCA+TGG 0.590641 5.1:-8523369 MS.gene20258:CDS
AAGACATAAATCTGGTCAGA+GGG 0.592633 5.1:+8523490 None:intergenic
ATCCGGCCAAGTGAGACTGC+AGG 0.593487 5.1:-8522365 MS.gene20258:CDS
AAGGACTTACACCAAACGTA+TGG 0.593798 5.1:-8523806 MS.gene20258:CDS
TGGTGTAAGTCCTTAAGGTG+TGG 0.594467 5.1:+8523815 None:intergenic
CAAGACTTCAAGGAGAAGTA+CGG 0.597427 5.1:-8523940 MS.gene20258:CDS
AGCTGCCTAGAATCCAGGTG+AGG 0.614847 5.1:-8522439 MS.gene20258:CDS
TAGGATCCGGAAGACAGTGA+TGG 0.617671 5.1:-8523644 MS.gene20258:CDS
ATACACCGTTAAAGAAACTC+AGG 0.619813 5.1:-8523114 MS.gene20258:intron
GCTGCCTAGAATCCAGGTGA+GGG 0.628324 5.1:-8522438 MS.gene20258:CDS
GTTCTTCCCGTTGAATGACA+AGG 0.631293 5.1:-8523845 MS.gene20258:CDS
GAAGACATAAATCTGGTCAG+AGG 0.641158 5.1:+8523489 None:intergenic
AGAGTTCAAGGAGAAATATG+GGG 0.671436 5.1:-8523563 MS.gene20258:CDS
CGTGACAGGAATTACCTCGT+AGG 0.675089 5.1:-8523613 MS.gene20258:CDS
GATTTATGTCTTCTTCCCGG+AGG 0.681678 5.1:-8523479 MS.gene20258:CDS
AGAAGACCTTGTCATCAACA+AGG 0.684606 5.1:-8523527 MS.gene20258:CDS
TAACCTCAAAATCTCCTACG+AGG 0.757108 5.1:+8523599 None:intergenic
TAACCTCGAAATCTTCTACG+AGG 0.763929 5.1:+8523968 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCACTATATCTAATATTTA+TGG - chr5.1:8523910-8523929 MS.gene20258:CDS 15.0%
!! AAAAACTTCCAAATGAAATT+TGG + chr5.1:8522683-8522702 None:intergenic 20.0%
!! TATATAATAGTATTTCACCT+AGG - chr5.1:8523355-8523374 MS.gene20258:CDS 20.0%
!! TTTATTTGCATGAAATACAA+TGG + chr5.1:8523665-8523684 None:intergenic 20.0%
!!! GATCAATCAATCAAATTTTA+TGG - chr5.1:8523098-8523117 MS.gene20258:intron 20.0%
!!! TATAACTTTACTTTAGAATC+AGG + chr5.1:8523473-8523492 None:intergenic 20.0%
! AGATAATATGCAAACAAGAA+CGG + chr5.1:8523838-8523857 None:intergenic 25.0%
! ATGAAATAATGAAGCTGTAT+AGG - chr5.1:8522350-8522369 MS.gene20258:CDS 25.0%
! CCAATGTAAATAGATTCAAA+AGG + chr5.1:8523762-8523781 None:intergenic 25.0%
! GTCAGTTATAAAAAACATTG+TGG - chr5.1:8523290-8523309 MS.gene20258:intron 25.0%
! TCCAAATCTAAATTAGAATC+AGG + chr5.1:8523607-8523626 None:intergenic 25.0%
! TCCTGATTCTAATTTAGATT+TGG - chr5.1:8523603-8523622 MS.gene20258:CDS 25.0%
!! AATTTTATGGTGGTTGTTAA+TGG - chr5.1:8523111-8523130 MS.gene20258:intron 25.0%
!! ATTTCATTTGGAAGTTTTTC+AGG - chr5.1:8522684-8522703 MS.gene20258:intron 25.0%
!! CAATCAATCAAATTTTATGG+TGG - chr5.1:8523101-8523120 MS.gene20258:intron 25.0%
!! CCTTTTGAATCTATTTACAT+TGG - chr5.1:8523759-8523778 MS.gene20258:CDS 25.0%
AAATAGATTCAAAAGGTCTC+AGG + chr5.1:8523755-8523774 None:intergenic 30.0%
AACACATAAATCTGGTCAAA+GGG + chr5.1:8522520-8522539 None:intergenic 30.0%
AACTAGATTGTACATCATCA+TGG + chr5.1:8523450-8523469 None:intergenic 30.0%
AACTTCCAAATGAAATTTGG+AGG + chr5.1:8522680-8522699 None:intergenic 30.0%
AAGAGTTCAAGGAGAAATAT+GGG - chr5.1:8522818-8522837 MS.gene20258:intron 30.0%
ATGAAATATCGAAGCTGTAT+AGG - chr5.1:8522719-8522738 MS.gene20258:intron 30.0%
CTGCTTTAATCTTTGTCTAA+TGG - chr5.1:8523807-8523826 MS.gene20258:CDS 30.0%
! GGAAGTTTTTCATGTGTTTT+CGG - chr5.1:8522693-8522712 MS.gene20258:intron 30.0%
! TTTACATTGGATAGTTCTAC+TGG - chr5.1:8523772-8523791 MS.gene20258:CDS 30.0%
! TTTTAACCTTTCTTCTTTCC+TGG - chr5.1:8523422-8523441 MS.gene20258:CDS 30.0%
!! CAGAAATTTTTCAAATGCCT+AGG + chr5.1:8523375-8523394 None:intergenic 30.0%
AAGACATAAATCTGGTCAGA+GGG + chr5.1:8522895-8522914 None:intergenic 35.0%
AATCGCCTCCAAATTTCATT+TGG - chr5.1:8522672-8522691 MS.gene20258:intron 35.0%
ACTGACCTGAGTTTCTTTAA+CGG + chr5.1:8523276-8523295 None:intergenic 35.0%
AGAGTTCAAGGAGAAATATG+GGG - chr5.1:8522819-8522838 MS.gene20258:intron 35.0%
ATACACCGTTAAAGAAACTC+AGG - chr5.1:8523268-8523287 MS.gene20258:intron 35.0%
ATGCCATAATATCTTGCAAC+AGG + chr5.1:8523972-8523991 None:intergenic 35.0%
ATTGAACCAGGAAAGAAGAA+AGG + chr5.1:8523565-8523584 None:intergenic 35.0%
CAAGAGTTCAAGGAGAAATA+TGG - chr5.1:8522817-8522836 MS.gene20258:intron 35.0%
GAACACATAAATCTGGTCAA+AGG + chr5.1:8522521-8522540 None:intergenic 35.0%
GACAAGTTGTCAAGATAATC+CGG - chr5.1:8524000-8524019 MS.gene20258:CDS 35.0%
GATCCTGTTGCAAGATATTA+TGG - chr5.1:8523966-8523985 MS.gene20258:CDS 35.0%
GGTTAACATACAAGACTTCA+AGG - chr5.1:8522432-8522451 MS.gene20258:CDS 35.0%
GTTTGCCTCCAAATTTCATT+TGG - chr5.1:8523050-8523069 MS.gene20258:intron 35.0%
TAATGAAGCTGTATAGGATC+TGG - chr5.1:8522356-8522375 MS.gene20258:CDS 35.0%
TGTTCCATGACATGTTAGTA+AGG + chr5.1:8523020-8523039 None:intergenic 35.0%
TTGTACATCATCATTGAACC+AGG + chr5.1:8523577-8523596 None:intergenic 35.0%
! AAATTGTACTGACACTGACA+TGG - chr5.1:8523887-8523906 MS.gene20258:CDS 35.0%
! AGTTTTTCATGTGTTTTCGG+AGG - chr5.1:8522696-8522715 MS.gene20258:intron 35.0%
!!! GTCAAAGGGATTGTCTTTTT+TGG + chr5.1:8522506-8522525 None:intergenic 35.0%
!!! TTTTGGCTTTCTTGATGACA+AGG + chr5.1:8522489-8522508 None:intergenic 35.0%
AACGGTGTATCTCTCAAGTA+CGG + chr5.1:8523258-8523277 None:intergenic 40.0%
AAGGACTTACACCAAACGTA+TGG - chr5.1:8522576-8522595 MS.gene20258:intron 40.0%
ACAGCCTTACTAACATGTCA+TGG - chr5.1:8523013-8523032 MS.gene20258:intron 40.0%
ACATGTTAGTAAGGCTGTTC+TGG + chr5.1:8523011-8523030 None:intergenic 40.0%
ACGGGAAGAACACATAAATC+TGG + chr5.1:8522528-8522547 None:intergenic 40.0%
AGAAGACCTTGTCATCAACA+AGG - chr5.1:8522855-8522874 MS.gene20258:intron 40.0%
AGATATTATGGCATGAAGCG+TGG - chr5.1:8523978-8523997 MS.gene20258:CDS 40.0%
ATGGAACCAGGAAAGAAGAA+AGG + chr5.1:8523431-8523450 None:intergenic 40.0%
ATGGTGGTTGTTAATGGTTG+AGG - chr5.1:8523117-8523136 MS.gene20258:CDS 40.0%
ATTCTCGGAGTTCATACGTT+TGG + chr5.1:8522965-8522984 None:intergenic 40.0%
CAAGACTTCAAGGAGAAGTA+CGG - chr5.1:8522442-8522461 MS.gene20258:intron 40.0%
CCAGATTTATGTCTTCTTCC+CGG - chr5.1:8522900-8522919 MS.gene20258:intron 40.0%
GAAGACATAAATCTGGTCAG+AGG + chr5.1:8522896-8522915 None:intergenic 40.0%
TAACCTCAAAATCTCCTACG+AGG + chr5.1:8522786-8522805 None:intergenic 40.0%
TAACCTCGAAATCTTCTACG+AGG + chr5.1:8522417-8522436 None:intergenic 40.0%
TATCGAAGCTGTATAGGATC+CGG - chr5.1:8522725-8522744 MS.gene20258:intron 40.0%
TTACCTCGTAGAAGATTTCG+AGG - chr5.1:8522411-8522430 MS.gene20258:CDS 40.0%
TTGTACATCATCATGGAACC+AGG + chr5.1:8523443-8523462 None:intergenic 40.0%
TTTCTCCTTGAACTCTTGCT+TGG + chr5.1:8522815-8522834 None:intergenic 40.0%
! TTACCTCGTAGGAGATTTTG+AGG - chr5.1:8522780-8522799 MS.gene20258:intron 40.0%
!! ACGTTTGGTGTAAGTCCTTA+AGG + chr5.1:8522575-8522594 None:intergenic 40.0%
!!! GTCAGAGGGATTGTCTTTTT+TGG + chr5.1:8522881-8522900 None:intergenic 40.0%
!!! TTTTGGCCTTGTTGATGACA+AGG + chr5.1:8522864-8522883 None:intergenic 40.0%
ACGCCAACCTTGTCATTCAA+CGG + chr5.1:8522547-8522566 None:intergenic 45.0%
ATTCTCGGAGTCCATACGTT+TGG + chr5.1:8522590-8522609 None:intergenic 45.0%
CATGTCCAAGCAAGAGTTCA+AGG - chr5.1:8522807-8522826 MS.gene20258:intron 45.0%
CCGGGAAGAAGACATAAATC+TGG + chr5.1:8522903-8522922 None:intergenic 45.0%
GATTTATGTCTTCTTCCCGG+AGG - chr5.1:8522903-8522922 MS.gene20258:intron 45.0%
GCTGTATAGGATCTGGAAGA+AGG - chr5.1:8522363-8522382 MS.gene20258:CDS 45.0%
GTTCTTCCCGTTGAATGACA+AGG - chr5.1:8522537-8522556 MS.gene20258:intron 45.0%
TAGGATCTGGAAGAAGGTGA+TGG - chr5.1:8522369-8522388 MS.gene20258:CDS 45.0%
TATCTTCCTGCAGTCTCACT+TGG + chr5.1:8524026-8524045 None:intergenic 45.0%
TGGTGTAAGTCCTGAAAGTC+TGG + chr5.1:8522945-8522964 None:intergenic 45.0%
TGGTGTAAGTCCTTAAGGTG+TGG + chr5.1:8522570-8522589 None:intergenic 45.0%
TTCCCGTTGAATGACAAGGT+TGG - chr5.1:8522541-8522560 MS.gene20258:intron 45.0%
! GATGGCTCTGTAGACATTCT+CGG + chr5.1:8522605-8522624 None:intergenic 45.0%
ATCTCAGCTGCCTAGAATCC+AGG - chr5.1:8523938-8523957 MS.gene20258:CDS 50.0%
CAAGCTTGTGCCAGAACATC+AGG - chr5.1:8523208-8523227 MS.gene20258:CDS 50.0%
CCAGACCAGCCTTACTAAAG+CGG - chr5.1:8522633-8522652 MS.gene20258:intron 50.0%
CGCCAACCTTGTCATTCAAC+GGG + chr5.1:8522546-8522565 None:intergenic 50.0%
CGTGACAGGAATTACCTCGT+AGG - chr5.1:8522769-8522788 MS.gene20258:intron 50.0%
GCATCTCCATCACTGTCTTC+CGG + chr5.1:8522747-8522766 None:intergenic 50.0%
GCTGTTCTGGCAAACGATGA+TGG + chr5.1:8522998-8523017 None:intergenic 50.0%
TAGGATCCGGAAGACAGTGA+TGG - chr5.1:8522738-8522757 MS.gene20258:intron 50.0%
TCAGGACAAGCGAACGACAA+AGG + chr5.1:8523737-8523756 None:intergenic 50.0%
TCAGTCAGCACCTGATGTTC+TGG + chr5.1:8523221-8523240 None:intergenic 50.0%
TGATGGAGATGCTTCGTGAC+AGG - chr5.1:8522755-8522774 MS.gene20258:intron 50.0%
!! AGTCCAGTGCCGCTTTAGTA+AGG + chr5.1:8522645-8522664 None:intergenic 50.0%
ACGCCAACCTTGTCATCCTC+CGG + chr5.1:8522922-8522941 None:intergenic 55.0%
AGCTGCCTAGAATCCAGGTG+AGG - chr5.1:8523943-8523962 MS.gene20258:CDS 55.0%
AGGTTGGCGTCCACACCTTA+AGG - chr5.1:8522557-8522576 MS.gene20258:intron 55.0%
AGGTTGGCGTCCAGACTTTC+AGG - chr5.1:8522932-8522951 MS.gene20258:intron 55.0%
CAGCCTTACTAAAGCGGCAC+TGG - chr5.1:8522639-8522658 MS.gene20258:intron 55.0%
CTTCTTCCCGGAGGATGACA+AGG - chr5.1:8522912-8522931 MS.gene20258:intron 55.0%
CTTGCAGGAGGCTGAATTGC+TGG - chr5.1:8523169-8523188 MS.gene20258:CDS 55.0%
GCTGCCTAGAATCCAGGTGA+GGG - chr5.1:8523944-8523963 MS.gene20258:CDS 55.0%
GGATCCCTCACCTGGATTCT+AGG + chr5.1:8523951-8523970 None:intergenic 55.0%
TGATGGAGATGCTTCGCGAC+AGG - chr5.1:8522386-8522405 MS.gene20258:CDS 55.0%
TTCCCGGAGGATGACAAGGT+TGG - chr5.1:8522916-8522935 MS.gene20258:intron 55.0%
TTCCTGCAGTCTCACTTGGC+CGG + chr5.1:8524022-8524041 None:intergenic 55.0%
TTGCAACAGGATCCCTCACC+TGG + chr5.1:8523959-8523978 None:intergenic 55.0%
! CCGCTTTAGTAAGGCTGGTC+TGG + chr5.1:8522636-8522655 None:intergenic 55.0%
! GCTGGTCTGGCAAACGATGA+TGG + chr5.1:8522623-8522642 None:intergenic 55.0%
!! CAGTGCCGCTTTAGTAAGGC+TGG + chr5.1:8522641-8522660 None:intergenic 55.0%
ATCCGGCCAAGTGAGACTGC+AGG - chr5.1:8524017-8524036 MS.gene20258:CDS 60.0%
CGCCAACCTTGTCATCCTCC+GGG + chr5.1:8522921-8522940 None:intergenic 60.0%
GTGTGTGTGCGCGCGCTTGC+AGG - chr5.1:8523154-8523173 MS.gene20258:CDS 70.0%
TGTGTGCGCGCGCTTGCAGG+AGG - chr5.1:8523157-8523176 MS.gene20258:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 8522334 8524070 8522334 ID=MS.gene20258
chr5.1 mRNA 8522334 8524070 8522334 ID=MS.gene20258.t1;Parent=MS.gene20258
chr5.1 exon 8523321 8524070 8523321 ID=MS.gene20258.t1.exon1;Parent=MS.gene20258.t1
chr5.1 CDS 8523321 8524070 8523321 ID=cds.MS.gene20258.t1;Parent=MS.gene20258.t1
chr5.1 exon 8523115 8523228 8523115 ID=MS.gene20258.t1.exon2;Parent=MS.gene20258.t1
chr5.1 CDS 8523115 8523228 8523115 ID=cds.MS.gene20258.t1;Parent=MS.gene20258.t1
chr5.1 exon 8522334 8522459 8522334 ID=MS.gene20258.t1.exon3;Parent=MS.gene20258.t1
chr5.1 CDS 8522334 8522459 8522334 ID=cds.MS.gene20258.t1;Parent=MS.gene20258.t1
Gene Sequence

>MS.gene20258

ATGGTGTTTTCGGAGGATGAAATAATGAAGCTGTATAGGATCTGGAAGAAGGTGATGGAGATGCTTCGCGACAGGAATTACCTCGTAGAAGATTTCGAGGTTAACATACAAGACTTCAAGGAGAAGTACGGTGAGAATGTGAAGAGAGAAGACCTTGTCATCAAGAAAGCCAAAAAAGACAATCCCTTTGACCAGATTTATGTGTTCTTCCCGTTGAATGACAAGGTTGGCGTCCACACCTTAAGGACTTACACCAAACGTATGGACTCCGAGAATGTCTACAGAGCCATCATCGTTTGCCAGACCAGCCTTACTAAAGCGGCACTGGACTTCTCTGAAATCGCCTCCAAATTTCATTTGGAAGTTTTTCATGTGTTTTCGGAGGATGAAATATCGAAGCTGTATAGGATCCGGAAGACAGTGATGGAGATGCTTCGTGACAGGAATTACCTCGTAGGAGATTTTGAGGTTAACATGTCCAAGCAAGAGTTCAAGGAGAAATATGGGGAGCACATGAAGAGAGAAGACCTTGTCATCAACAAGGCCAAAAAAGACAATCCCTCTGACCAGATTTATGTCTTCTTCCCGGAGGATGACAAGGTTGGCGTCCAGACTTTCAGGACTTACACCAAACGTATGAACTCCGAGAATGTCTACAGAGCCATCATCGTTTGCCAGAACAGCCTTACTAACATGTCATGGAACAGTATCTCTCAGTTTGCCTCCAAATTTCATTTGGAAGTTTTTCAGGTAATTCATCAGTTGATCAATCAATCAAATTTTATGGTGGTTGTTAATGGTTGAGGTAACTGTTGTTAGTGTGTGTGTGCGCGCGCTTGCAGGAGGCTGAATTGCTGGTTAATGTAAGATATCACAAGCTTGTGCCAGAACATCAGGTGCTGACTGACAATGAGAAGAATTCCGTACTTGAGAGATACACCGTTAAAGAAACTCAGGTCAGTTATAAAAAACATTGTGGAAATTTTGTGTTATAGAATGTGACTAATCTAATGAGTAAAGTTATATAATAGTATTTCACCTAGGCATTTGAAAAATTTCTGCACACGCACAAGTGCTTCGTCTCTAATTTTTAACCTTTCTTCTTTCCTGGTTCCATGATGATGTACAATCTAGTTCCTGATTCTAAAGTAAAGTTATATAATAGTATTTCACCTAGGCATTTGAAAAATTTCTGCACACGCACAAGTGCTTCGTCTCTAATTTTTAACCTTTCTTCTTTCCTGGTTCAATGATGATGTACAATCTAGTTCCTGATTCTAATTTAGATTTGGATGTAATTAAGAATGCATGAAAAGTAAAATTAGCATCCATTGTATTTCATGCAAATAAAACTTCTTTTCATTGTTTATTTCTATAGTGAAAGTTTGTCAGATTTGTCACCTTTGTCGTTCGCTTGTCCTGAGACCTTTTGAATCTATTTACATTGGATAGTTCTACTGGTTTTAGTGTTTACTGCTTTAATCTTTGTCTAATGGATGTACCGTTCTTGTTTGCATATTATCTCGTATTTGCAATTGTATCATTTTACTACAAAATTGTACTGACACTGACATGGTTCACTATATCTAATATTTATGGTCTGTATCTCAGCTGCCTAGAATCCAGGTGAGGGATCCTGTTGCAAGATATTATGGCATGAAGCGTGGACAAGTTGTCAAGATAATCCGGCCAAGTGAGACTGCAGGAAGATATGTTACATACCGATTTGTTGTCTAA
Protein sequence

>MS.gene20258.t1

MVFSEDEIMKLYRIWKKVMEMLRDRNYLVEDFEVNIQDFKEKYGENVKREDLVIKKAKKDNPFDQIYVFFPLNDKVGVHTLRTYTKRMDSENVYRAIIVCQTSLTKAALDFSEIASKFHLEVFHVFSEDEISKLYRIRKTVMEMLRDRNYLVGDFEVNMSKQEFKEKYGEHMKREDLVINKAKKDNPSDQIYVFFPEDDKVGVQTFRTYTKRMNSENVYRAIIVCQNSLTNMSWNSISQFASKFHLEVFQEAELLVNVRYHKLVPEHQVLTDNEKNSVLERYTVKETQLPRIQVRDPVARYYGMKRGQVVKIIRPSETAGRYVTYRFVV