AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene04856

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04856.t1 MTR_7g092450 100.000 145 0 0 1 145 1 145 2.53e-100 284
MS.gene04856.t1 MTR_0019s0160 83.448 145 23 1 1 145 1 144 5.71e-73 215
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04856.t1 AT5G51940 79.452 146 27 2 1 145 1 144 1.97e-70 208
MS.gene04856.t1 AT2G04630 78.082 146 29 2 1 145 1 144 6.51e-69 204

Find 57 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATACTTGGAAGTCCTTC+TGG 0.228416 7.2:+16657825 None:intergenic
CCGCTCTCGTAGCTCCTTCA+TGG 0.261897 7.2:+16655292 None:intergenic
AAGTATGGTACCTCAATTTC+AGG 0.283991 7.2:+16658011 None:intergenic
AGGTCATCATTCTTGTTATC+AGG 0.290460 7.2:+16657910 None:intergenic
ACCACCCACTTGTCTCTTCC+AGG 0.359364 7.2:+16655070 None:intergenic
AAGTAGCGACGGATGGTAAA+AGG 0.371530 7.2:+16655263 None:intergenic
GTCCTTCTGGGACGTTCAAC+CGG 0.388301 7.2:+16657838 None:intergenic
GTACCCGCGCTCTTCAAATC+AGG 0.398664 7.2:-16657774 MS.gene04856:intron
AACTCTTCAGATTGCCATGA+AGG 0.418174 7.2:-16655306 MS.gene04856:intron
ATTGTCCAGCTATGAAGATT+GGG 0.418900 7.2:-16655120 MS.gene04856:intron
ACCAATTGAAACTGAGGAAA+AGG 0.424157 7.2:-16657872 MS.gene04856:CDS
TATGAGCGTGCTAGGATTCT+CGG 0.433316 7.2:-16657796 MS.gene04856:CDS
AATTGTCCAGCTATGAAGAT+TGG 0.435283 7.2:-16655121 MS.gene04856:intron
CTCATACTTAGTCATATACT+TGG 0.444475 7.2:+16657813 None:intergenic
ACCTGTCATGGTTGAGTTGG+AGG 0.445018 7.2:-16656370 MS.gene04856:CDS
AGCTGATTGTGGAAGACTCC+TGG 0.445700 7.2:-16655088 MS.gene04856:CDS
GGTACCTCAATTTCAGGCTC+TGG 0.453336 7.2:+16658017 None:intergenic
AGTAGTATTTACCTTCCATC+AGG 0.479574 7.2:+16655239 None:intergenic
GCCACCAGAGCCTGAAATTG+AGG 0.481155 7.2:-16658021 MS.gene04856:intron
ATATACTTGGAAGTCCTTCT+GGG 0.481308 7.2:+16657826 None:intergenic
CCCTCCAACTCAACCATGAC+AGG 0.481823 7.2:+16656369 None:intergenic
TTCGTAGTCATCGTCGGCCA+TGG 0.482815 7.2:+16658158 None:intergenic
TTGGTTCTCCTTCTGCATCT+AGG 0.484900 7.2:+16657890 None:intergenic
AATGGTTAAATTACCTCAAG+TGG 0.493654 7.2:+16656336 None:intergenic
CCTGTCATGGTTGAGTTGGA+GGG 0.500999 7.2:-16656369 MS.gene04856:CDS
GCAGGAAGGTGCAGAGGAGG+AGG 0.505888 7.2:-16657935 MS.gene04856:intron
ACCTCAATTTCAGGCTCTGG+TGG 0.507724 7.2:+16658020 None:intergenic
GGGAGTTGATGAGCTGATTG+TGG 0.508386 7.2:-16655099 MS.gene04856:CDS
GGAAAAGGAAGATGCTGCTC+CGG 0.509327 7.2:-16657857 MS.gene04856:CDS
TGACTAAGTATGAGCGTGCT+AGG 0.520533 7.2:-16657804 MS.gene04856:CDS
ATCCGTCGCTACTTGCCTGA+TGG 0.540830 7.2:-16655254 MS.gene04856:CDS
TGCACCTGTCATGGTTGAGT+TGG 0.547769 7.2:-16656373 MS.gene04856:CDS
TTCCATCAGGCAAGTAGCGA+CGG 0.559984 7.2:+16655252 None:intergenic
TGTGCAGGAAGGTGCAGAGG+AGG 0.563259 7.2:-16657938 MS.gene04856:intron
GTCGCTACTTGCCTGATGGA+AGG 0.581718 7.2:-16655250 MS.gene04856:intron
AAACCTGATTTGAAGAGCGC+GGG 0.585792 7.2:+16657771 None:intergenic
GACTCCTGGAAGAGACAAGT+GGG 0.586530 7.2:-16655074 MS.gene04856:CDS
AGGAGAACCAATTGAAACTG+AGG 0.593065 7.2:-16657878 MS.gene04856:CDS
CAGTATGAATGCACCTGTCA+TGG 0.596445 7.2:-16656382 MS.gene04856:CDS
CTCCGGTTGAACGTCCCAGA+AGG 0.600340 7.2:-16657840 MS.gene04856:CDS
ATCAGGCAAGTAGCGACGGA+TGG 0.606515 7.2:+16655256 None:intergenic
CTGTCATGGTTGAGTTGGAG+GGG 0.614709 7.2:-16656368 MS.gene04856:CDS
AATGATGACCTAGATGCAGA+AGG 0.620982 7.2:-16657898 MS.gene04856:CDS
TGTCATGGTTGAGTTGGAGG+GGG 0.622677 7.2:-16656367 MS.gene04856:CDS
TAAACCTGATTTGAAGAGCG+CGG 0.623407 7.2:+16657770 None:intergenic
GACTACGAAGATATCGACAT+GGG 0.632471 7.2:-16658144 MS.gene04856:CDS
ATGGTTAAATTACCTCAAGT+GGG 0.636541 7.2:+16656337 None:intergenic
AGACTCCTGGAAGAGACAAG+TGG 0.644950 7.2:-16655075 MS.gene04856:CDS
TTGTCCAGCTATGAAGATTG+GGG 0.646097 7.2:-16655119 MS.gene04856:intron
GATATCTTCGTAGTCATCGT+CGG 0.659789 7.2:+16658152 None:intergenic
TGACTACGAAGATATCGACA+TGG 0.677574 7.2:-16658145 MS.gene04856:CDS
GCATGTGCAGGAAGGTGCAG+AGG 0.686778 7.2:-16657941 MS.gene04856:intron
ATTAAAACGATGCAGGTACG+AGG 0.688503 7.2:-16658048 MS.gene04856:intron
CCATGAAGGAGCTACGAGAG+CGG 0.693186 7.2:-16655292 MS.gene04856:CDS
AAAACGATGCAGGTACGAGG+AGG 0.694919 7.2:-16658045 MS.gene04856:intron
TCCTGGAAGAGACAAGTGGG+TGG 0.726072 7.2:-16655071 MS.gene04856:CDS
ACTACGAAGATATCGACATG+GGG 0.760905 7.2:-16658143 MS.gene04856:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CACATAATTAATAATTAATT+AGG - chr7.2:16655126-16655145 MS.gene04856:intron 10.0%
!!! AATAGGCTTATTTTTATATT+AGG - chr7.2:16657288-16657307 MS.gene04856:intron 15.0%
!!! ATGATTGTTTAAGATTTTAA+AGG + chr7.2:16656770-16656789 None:intergenic 15.0%
!!! CTTCTTTTTTTCTTTAATTA+GGG - chr7.2:16655232-16655251 MS.gene04856:intron 15.0%
!!! TCTTCTTTTTTTCTTTAATT+AGG - chr7.2:16655231-16655250 MS.gene04856:intron 15.0%
!! AAAGAAAAAAAGAAGAAGTA+TGG + chr7.2:16655227-16655246 None:intergenic 20.0%
!! AAATTGAACTATGTTAACAT+GGG - chr7.2:16655715-16655734 MS.gene04856:intron 20.0%
!! AATAAGCAAAATCATACATA+GGG + chr7.2:16655492-16655511 None:intergenic 20.0%
!! ATTTATGTAACATGCAATAA+AGG + chr7.2:16656341-16656360 None:intergenic 20.0%
!! TAAATTGAACTATGTTAACA+TGG - chr7.2:16655714-16655733 MS.gene04856:intron 20.0%
!! TACTGTTTGTTTATTAAGTT+GGG - chr7.2:16658000-16658019 MS.gene04856:intron 20.0%
!! TGAAAATTATAGCATAAACT+GGG - chr7.2:16657434-16657453 MS.gene04856:intron 20.0%
!! TTAAATCCTAAATTAACATG+TGG + chr7.2:16657602-16657621 None:intergenic 20.0%
!! TTAACATATACTTGTGTAAT+AGG - chr7.2:16655745-16655764 MS.gene04856:intron 20.0%
!! TTAGGATTTAAATATTCTCA+TGG - chr7.2:16657611-16657630 MS.gene04856:intron 20.0%
!! TTGAAAATTATAGCATAAAC+TGG - chr7.2:16657433-16657452 MS.gene04856:intron 20.0%
!! TTTAAAAAAAGTTTCATCGA+TGG + chr7.2:16657500-16657519 None:intergenic 20.0%
!! TTTCTATATTTCAATTGAGT+GGG + chr7.2:16657019-16657038 None:intergenic 20.0%
!!! TTTTCTATATTTCAATTGAG+TGG + chr7.2:16657020-16657039 None:intergenic 20.0%
! AAACAACTTATCAATAGAGT+AGG - chr7.2:16656424-16656443 MS.gene04856:intron 25.0%
! AAGTTGTCAAACAAAATTTG+AGG - chr7.2:16656042-16656061 MS.gene04856:intron 25.0%
! ACATACATGTATAGTAACAA+GGG - chr7.2:16657198-16657217 MS.gene04856:intron 25.0%
! ACTCTATTGATAAGTTGTTT+GGG + chr7.2:16656424-16656443 None:intergenic 25.0%
! AGATCAATAAAGTAGAAGTA+AGG + chr7.2:16657061-16657080 None:intergenic 25.0%
! ATTAGTATTTGTGTTAGCTA+TGG - chr7.2:16655513-16655532 MS.gene04856:intron 25.0%
! CTAACAAAAAGTTCTTTCAA+CGG + chr7.2:16656747-16656766 None:intergenic 25.0%
! CTACTGTTTGTTTATTAAGT+TGG - chr7.2:16657999-16658018 MS.gene04856:intron 25.0%
! GAATAAGCAAAATCATACAT+AGG + chr7.2:16655493-16655512 None:intergenic 25.0%
! TACTCTATTGATAAGTTGTT+TGG + chr7.2:16656425-16656444 None:intergenic 25.0%
! TGATAATTAAGTTCTTGTTG+AGG - chr7.2:16655935-16655954 MS.gene04856:intron 25.0%
! TTCTATATTTCAATTGAGTG+GGG + chr7.2:16657018-16657037 None:intergenic 25.0%
!! AACTTTTATGTTGGAGAATA+TGG + chr7.2:16655961-16655980 None:intergenic 25.0%
!! ACATAAATTGACAAGCTTTT+TGG - chr7.2:16656353-16656372 MS.gene04856:CDS 25.0%
!! TGCTATAATTTTCAAAGTTC+AGG + chr7.2:16657428-16657447 None:intergenic 25.0%
!! TGTATGTTCTAAATAGAGTA+AGG + chr7.2:16655687-16655706 None:intergenic 25.0%
!! TTGCAACTTTTATTGAAGTA+TGG + chr7.2:16656476-16656495 None:intergenic 25.0%
!!! AAAAATAAGCCTATTTCTGA+TGG + chr7.2:16657283-16657302 None:intergenic 25.0%
!!! ATCAATGGGTTATGTAAAAT+GGG - chr7.2:16655543-16655562 MS.gene04856:intron 25.0%
!!! GTTTTATTTTTAGAGTCCTT+TGG - chr7.2:16657650-16657669 MS.gene04856:intron 25.0%
!!! TCCATATTTTTTTGTTGTGT+TGG - chr7.2:16657813-16657832 MS.gene04856:CDS 25.0%
AATGGTTAAATTACCTCAAG+TGG + chr7.2:16656887-16656906 None:intergenic 30.0%
ACAAGAAAGACAACAAGTTA+TGG + chr7.2:16656810-16656829 None:intergenic 30.0%
ACATGTATGTCAGCATATAT+TGG + chr7.2:16657188-16657207 None:intergenic 30.0%
ACTGATACAAAATGAAACCA+TGG + chr7.2:16657844-16657863 None:intergenic 30.0%
ATAACATGTAGCAATACCAA+AGG + chr7.2:16657669-16657688 None:intergenic 30.0%
ATACAAACAAGATCAACATC+TGG + chr7.2:16657128-16657147 None:intergenic 30.0%
ATCAATGGATGATGTAAAAC+AGG + chr7.2:16655596-16655615 None:intergenic 30.0%
ATCTGACCACATGTTAATTT+AGG - chr7.2:16657593-16657612 MS.gene04856:intron 30.0%
ATCTTAAACAATCATACTGC+AGG - chr7.2:16656775-16656794 MS.gene04856:intron 30.0%
ATGGTTAAATTACCTCAAGT+GGG + chr7.2:16656886-16656905 None:intergenic 30.0%
ATTGCAATATGAACCTTACA+AGG + chr7.2:16656399-16656418 None:intergenic 30.0%
CTCATACTTAGTCATATACT+TGG + chr7.2:16655410-16655429 None:intergenic 30.0%
CTCTATTGATAAGTTGTTTG+GGG + chr7.2:16656423-16656442 None:intergenic 30.0%
CTGAACAAATGAACTATAAC+AGG + chr7.2:16657325-16657344 None:intergenic 30.0%
GACATACATGTATAGTAACA+AGG - chr7.2:16657197-16657216 MS.gene04856:intron 30.0%
GATCAAATTCCATCAGAAAT+AGG - chr7.2:16657271-16657290 MS.gene04856:intron 30.0%
GCCAACACAACAAAAAAATA+TGG + chr7.2:16657817-16657836 None:intergenic 30.0%
TAAGAAATCTACATACGTGT+CGG - chr7.2:16655897-16655916 MS.gene04856:intron 30.0%
TGATCTCGTCTTCAAAATAT+AGG + chr7.2:16657256-16657275 None:intergenic 30.0%
TTTACATCATCCATTGATCA+AGG - chr7.2:16655598-16655617 MS.gene04856:intron 30.0%
! AGCTTTTTGGACAACAAAAT+AGG - chr7.2:16656366-16656385 MS.gene04856:CDS 30.0%
! CTTTCTCCCTTTAAAGAAAA+AGG - chr7.2:16657401-16657420 MS.gene04856:intron 30.0%
! TATGCTTAGATCATTGACTA+TGG - chr7.2:16657145-16657164 MS.gene04856:intron 30.0%
! TCAGGACCTTTTTCTTTAAA+GGG + chr7.2:16657410-16657429 None:intergenic 30.0%
! TTATGTCGTACTTACCATTT+TGG - chr7.2:16658075-16658094 MS.gene04856:intron 30.0%
! TTCAGGACCTTTTTCTTTAA+AGG + chr7.2:16657411-16657430 None:intergenic 30.0%
! TTTTATTGAAGTATGGTGCT+TGG + chr7.2:16656469-16656488 None:intergenic 30.0%
!! GATCAATGGGTTATGTAAAA+TGG - chr7.2:16655542-16655561 MS.gene04856:intron 30.0%
AAAGTACAAGCTATGGACAA+TGG + chr7.2:16657368-16657387 None:intergenic 35.0%
AAGTACGACATAAAAGATGG+AGG + chr7.2:16658068-16658087 None:intergenic 35.0%
AAGTATGGTACCTCAATTTC+AGG + chr7.2:16655212-16655231 None:intergenic 35.0%
AATTGTCCAGCTATGAAGAT+TGG - chr7.2:16658099-16658118 MS.gene04856:intron 35.0%
ACCAATTGAAACTGAGGAAA+AGG - chr7.2:16655348-16655367 MS.gene04856:intron 35.0%
ACTCATGATTAAAACGATGC+AGG - chr7.2:16655165-16655184 MS.gene04856:intron 35.0%
AGCTATGGAATCTTGATCAA+TGG - chr7.2:16655528-16655547 MS.gene04856:intron 35.0%
AGCTGTAAAAACGAATACTC+AGG + chr7.2:16656308-16656327 None:intergenic 35.0%
AGGGGAGAATTTAAACCAAA+CGG + chr7.2:16656660-16656679 None:intergenic 35.0%
AGTAGTATTTACCTTCCATC+AGG + chr7.2:16657984-16658003 None:intergenic 35.0%
ATAGAGTAAGGATGAGAAAG+AGG + chr7.2:16655675-16655694 None:intergenic 35.0%
ATATACTTGGAAGTCCTTCT+GGG + chr7.2:16655397-16655416 None:intergenic 35.0%
ATGTGCAATTGTGCATTACA+TGG + chr7.2:16657102-16657121 None:intergenic 35.0%
ATTGTCCAGCTATGAAGATT+GGG - chr7.2:16658100-16658119 MS.gene04856:intron 35.0%
CAAAAATCCAGAATAGATGC+TGG - chr7.2:16656217-16656236 MS.gene04856:intron 35.0%
CAGTACTGTAACAAGAAAGA+AGG + chr7.2:16656931-16656950 None:intergenic 35.0%
CATGGTACACTACTTAATGA+AGG + chr7.2:16657084-16657103 None:intergenic 35.0%
CATTGATCAAGGTAACCAAA+AGG - chr7.2:16655609-16655628 MS.gene04856:intron 35.0%
CATTTGTTCAGCTTGTAGAT+TGG - chr7.2:16657334-16657353 MS.gene04856:intron 35.0%
GCATAGTAGTGTATTGTACT+GGG + chr7.2:16656584-16656603 None:intergenic 35.0%
GCTATGGAATCTTGATCAAT+GGG - chr7.2:16655529-16655548 MS.gene04856:intron 35.0%
GGTAAGTACGACATAAAAGA+TGG + chr7.2:16658071-16658090 None:intergenic 35.0%
TGAATCGCAAAGAGATCAAA+TGG + chr7.2:16656905-16656924 None:intergenic 35.0%
TGATGTAAAACAGGACAAAC+TGG + chr7.2:16655587-16655606 None:intergenic 35.0%
TGCATAGTAGTGTATTGTAC+TGG + chr7.2:16656585-16656604 None:intergenic 35.0%
TTTCAAGAAGCTTGACAGTT+AGG + chr7.2:16656518-16656537 None:intergenic 35.0%
! AGGTCATCATTCTTGTTATC+AGG + chr7.2:16655313-16655332 None:intergenic 35.0%
! AGTTGGGTACTTCTTTTTCT+TGG - chr7.2:16658016-16658035 MS.gene04856:intron 35.0%
! CTTTTGTCAAAAAGTTGCCT+TGG + chr7.2:16656711-16656730 None:intergenic 35.0%
! GGAGTTGACAACTTTTATGT+TGG + chr7.2:16655970-16655989 None:intergenic 35.0%
! GGTACTTCTTTTTCTTGGAT+GGG - chr7.2:16658021-16658040 MS.gene04856:intron 35.0%
! TCCTTTTCCTCAGTTTCAAT+TGG + chr7.2:16655352-16655371 None:intergenic 35.0%
!! AAAATTTGAGGAGTGCTGAA+AGG - chr7.2:16656054-16656073 MS.gene04856:intron 35.0%
!! CTTTTGGTTACCTTGATCAA+TGG + chr7.2:16655611-16655630 None:intergenic 35.0%
AACTCTTCAGATTGCCATGA+AGG - chr7.2:16657914-16657933 MS.gene04856:CDS 40.0%
AATGATGACCTAGATGCAGA+AGG - chr7.2:16655322-16655341 MS.gene04856:intron 40.0%
ACAATGACTAAATGCCCGTT+TGG - chr7.2:16656642-16656661 MS.gene04856:intron 40.0%
ACACAGAAGCTGAAATCCTA+TGG + chr7.2:16656109-16656128 None:intergenic 40.0%
ACTACGAAGATATCGACATG+GGG - chr7.2:16655077-16655096 MS.gene04856:CDS 40.0%
ACTCCAGTGGTTTCTAATCT+AGG + chr7.2:16656142-16656161 None:intergenic 40.0%
AGACATCCAACGAAAATTCG+TGG + chr7.2:16655801-16655820 None:intergenic 40.0%
AGGAGAACCAATTGAAACTG+AGG - chr7.2:16655342-16655361 MS.gene04856:intron 40.0%
ATTAAAACGATGCAGGTACG+AGG - chr7.2:16655172-16655191 MS.gene04856:intron 40.0%
CATATACTTGGAAGTCCTTC+TGG + chr7.2:16655398-16655417 None:intergenic 40.0%
CGAATACTCAGGTATCTTAG+AGG + chr7.2:16656297-16656316 None:intergenic 40.0%
GACTACGAAGATATCGACAT+GGG - chr7.2:16655076-16655095 MS.gene04856:CDS 40.0%
GAGGATTGCATTATGAGACA+AGG - chr7.2:16656010-16656029 MS.gene04856:intron 40.0%
GATATCTTCGTAGTCATCGT+CGG + chr7.2:16655071-16655090 None:intergenic 40.0%
GCTTGACAGTTAGGTTAAGT+TGG + chr7.2:16656509-16656528 None:intergenic 40.0%
GGGGAGAATTTAAACCAAAC+GGG + chr7.2:16656659-16656678 None:intergenic 40.0%
GTAAAACAGGACAAACTGGA+AGG + chr7.2:16655583-16655602 None:intergenic 40.0%
TACAAGCTATGGACAATGGT+TGG + chr7.2:16657364-16657383 None:intergenic 40.0%
TAGCTGGACAATTGCCAAAA+TGG + chr7.2:16658092-16658111 None:intergenic 40.0%
TGAACATAGATCACAGCCAT+AGG - chr7.2:16656090-16656109 MS.gene04856:intron 40.0%
TGACTACGAAGATATCGACA+TGG - chr7.2:16655075-16655094 MS.gene04856:CDS 40.0%
TGGACAACAAAATAGGTTGC+AGG - chr7.2:16656373-16656392 MS.gene04856:CDS 40.0%
TTCATGGCAATCTGAAGAGT+TGG + chr7.2:16657915-16657934 None:intergenic 40.0%
TTGTCCAGCTATGAAGATTG+GGG - chr7.2:16658101-16658120 MS.gene04856:intron 40.0%
! AACCACTGGAGTTTTCTTCA+TGG - chr7.2:16656150-16656169 MS.gene04856:intron 40.0%
! ACTTCTTTTTCTTGGATGGG+AGG - chr7.2:16658024-16658043 MS.gene04856:CDS 40.0%
! AGCCAAGAAAGTACAAGCTA+TGG + chr7.2:16657375-16657394 None:intergenic 40.0%
! CTTCTTTTTCTTGGATGGGA+GGG - chr7.2:16658025-16658044 MS.gene04856:CDS 40.0%
! GGATTTTTGTGATGCAGACT+AGG + chr7.2:16656206-16656225 None:intergenic 40.0%
! GGGTACTTCTTTTTCTTGGA+TGG - chr7.2:16658020-16658039 MS.gene04856:intron 40.0%
! GTCCATAGCTTGTACTTTCT+TGG - chr7.2:16657370-16657389 MS.gene04856:intron 40.0%
! TAAACCTGATTTGAAGAGCG+CGG + chr7.2:16655453-16655472 None:intergenic 40.0%
! TAGCTTGTACTTTCTTGGCT+CGG - chr7.2:16657375-16657394 MS.gene04856:intron 40.0%
! TTGATGATTGATGCATGTGC+AGG - chr7.2:16655267-16655286 MS.gene04856:CDS 40.0%
! TTTCCTCCACGAATTTTCGT+TGG - chr7.2:16655792-16655811 MS.gene04856:intron 40.0%
AACTCCCCAATCTTCATAGC+TGG + chr7.2:16658108-16658127 None:intergenic 45.0%
AAGTAGCGACGGATGGTAAA+AGG + chr7.2:16657960-16657979 None:intergenic 45.0%
ACCATCCTGAGTTTGTCTCT+AGG - chr7.2:16655837-16655856 MS.gene04856:intron 45.0%
ACCTAGAGACAAACTCAGGA+TGG + chr7.2:16655841-16655860 None:intergenic 45.0%
AGCTTGTACTTTCTTGGCTC+GGG - chr7.2:16657376-16657395 MS.gene04856:intron 45.0%
AGTAAGGATGAGAAAGAGGC+TGG + chr7.2:16655671-16655690 None:intergenic 45.0%
AGTTGTCAACTCCACTTGCA+TGG - chr7.2:16655977-16655996 MS.gene04856:intron 45.0%
ATGGGGGAAAACACTTACCA+AGG - chr7.2:16656691-16656710 MS.gene04856:intron 45.0%
CACAACCTAGAGACAAACTC+AGG + chr7.2:16655845-16655864 None:intergenic 45.0%
CAGTATGAATGCACCTGTCA+TGG - chr7.2:16656838-16656857 MS.gene04856:intron 45.0%
CATCCAACGAAAATTCGTGG+AGG + chr7.2:16655798-16655817 None:intergenic 45.0%
CATGGTTCACTCCTACAGAA+CGG + chr7.2:16656257-16656276 None:intergenic 45.0%
CCACAAAAGAACCATGCAAG+TGG + chr7.2:16655991-16656010 None:intergenic 45.0%
CTGCCTAGATTAGAAACCAC+TGG - chr7.2:16656136-16656155 MS.gene04856:intron 45.0%
GACAGAACCAGCATCTATTC+TGG + chr7.2:16656227-16656246 None:intergenic 45.0%
GTCCATGAAGAAAACTCCAG+TGG + chr7.2:16656155-16656174 None:intergenic 45.0%
TATGAGCGTGCTAGGATTCT+CGG - chr7.2:16655424-16655443 MS.gene04856:intron 45.0%
TCTCTAGGTTGTGAAACGCA+GGG - chr7.2:16655852-16655871 MS.gene04856:intron 45.0%
TGACTAAGTATGAGCGTGCT+AGG - chr7.2:16655416-16655435 MS.gene04856:intron 45.0%
TGATTGATGCATGTGCAGGA+AGG - chr7.2:16655271-16655290 MS.gene04856:CDS 45.0%
TTTCTTGGATGGGAGGGTTA+AGG - chr7.2:16658031-16658050 MS.gene04856:CDS 45.0%
! AAACCTGATTTGAAGAGCGC+GGG + chr7.2:16655452-16655471 None:intergenic 45.0%
! AATAGGTTGCAGGCCTTGTA+AGG - chr7.2:16656383-16656402 MS.gene04856:intron 45.0%
! CATTGCTCTTGCTTGCCTTT+TGG + chr7.2:16655627-16655646 None:intergenic 45.0%
! CCACTTGCATGGTTCTTTTG+TGG - chr7.2:16655988-16656007 MS.gene04856:intron 45.0%
! CTTGCATGGTTCTTTTGTGG+AGG - chr7.2:16655991-16656010 MS.gene04856:intron 45.0%
! TGTCAGGTTTTCCGTTCTGT+AGG - chr7.2:16656243-16656262 MS.gene04856:intron 45.0%
! TTGGTTCTCCTTCTGCATCT+AGG + chr7.2:16655333-16655352 None:intergenic 45.0%
!! CTTAGAGGCACTATCACTCA+AGG + chr7.2:16656282-16656301 None:intergenic 45.0%
!! GAATAGATGCTGGTTCTGTC+AGG - chr7.2:16656227-16656246 MS.gene04856:intron 45.0%
!! TCTACATACGTGTCGGTTGT+TGG - chr7.2:16655904-16655923 MS.gene04856:intron 45.0%
!! ACATAATTAATAATTAATTA+GGG - chr7.2:16655127-16655146 MS.gene04856:intron 5.0%
AAAACGATGCAGGTACGAGG+AGG - chr7.2:16655175-16655194 MS.gene04856:intron 50.0%
AGACTCCTGGAAGAGACAAG+TGG - chr7.2:16658145-16658164 MS.gene04856:CDS 50.0%
AGCTGATTGTGGAAGACTCC+TGG - chr7.2:16658132-16658151 MS.gene04856:intron 50.0%
CGTTCTGTAGGAGTGAACCA+TGG - chr7.2:16656255-16656274 MS.gene04856:intron 50.0%
CTCTAGGTTGTGAAACGCAG+GGG - chr7.2:16655853-16655872 MS.gene04856:intron 50.0%
GACTCCTGGAAGAGACAAGT+GGG - chr7.2:16658146-16658165 MS.gene04856:CDS 50.0%
GCACTATCACTCAAGGACCA+TGG + chr7.2:16656275-16656294 None:intergenic 50.0%
GGAAAAGGAAGATGCTGCTC+CGG - chr7.2:16655363-16655382 MS.gene04856:intron 50.0%
GGTACCTCAATTTCAGGCTC+TGG + chr7.2:16655206-16655225 None:intergenic 50.0%
GTCTCTAGGTTGTGAAACGC+AGG - chr7.2:16655851-16655870 MS.gene04856:intron 50.0%
TCCCCCATAGTAGTCAGTGA+GGG + chr7.2:16656679-16656698 None:intergenic 50.0%
TGCACCTGTCATGGTTGAGT+TGG - chr7.2:16656847-16656866 MS.gene04856:intron 50.0%
TGTGTATCCCTAACCACCGA+TGG - chr7.2:16655645-16655664 MS.gene04856:intron 50.0%
TTCCATCAGGCAAGTAGCGA+CGG + chr7.2:16657971-16657990 None:intergenic 50.0%
TTCCCCCATAGTAGTCAGTG+AGG + chr7.2:16656680-16656699 None:intergenic 50.0%
! ACCTCAATTTCAGGCTCTGG+TGG + chr7.2:16655203-16655222 None:intergenic 50.0%
! ATGAGAAAGAGGCTGGCCAT+CGG + chr7.2:16655664-16655683 None:intergenic 50.0%
! CTCCCCTCACTGACTACTAT+GGG - chr7.2:16656673-16656692 MS.gene04856:intron 50.0%
! TCCCCTCACTGACTACTATG+GGG - chr7.2:16656674-16656693 MS.gene04856:intron 50.0%
! TCTCCCCTCACTGACTACTA+TGG - chr7.2:16656672-16656691 MS.gene04856:intron 50.0%
!! ACCTGTCATGGTTGAGTTGG+AGG - chr7.2:16656850-16656869 MS.gene04856:intron 50.0%
!! CCTGTCATGGTTGAGTTGGA+GGG - chr7.2:16656851-16656870 MS.gene04856:intron 50.0%
!! CTGTCATGGTTGAGTTGGAG+GGG - chr7.2:16656852-16656871 MS.gene04856:intron 50.0%
!! GCTTGGTGCAATCTCTAAGC+AGG + chr7.2:16656452-16656471 None:intergenic 50.0%
!! GGGAGTTGATGAGCTGATTG+TGG - chr7.2:16658121-16658140 MS.gene04856:intron 50.0%
!! TGTCATGGTTGAGTTGGAGG+GGG - chr7.2:16656853-16656872 MS.gene04856:intron 50.0%
!! TTGTTGTGTTGGCTCTGCCA+TGG - chr7.2:16657824-16657843 MS.gene04856:CDS 50.0%
ACCACCCACTTGTCTCTTCC+AGG + chr7.2:16658153-16658172 None:intergenic 55.0%
ATCAGGCAAGTAGCGACGGA+TGG + chr7.2:16657967-16657986 None:intergenic 55.0%
ATCCGTCGCTACTTGCCTGA+TGG - chr7.2:16657966-16657985 MS.gene04856:intron 55.0%
CCATGAAGGAGCTACGAGAG+CGG - chr7.2:16657928-16657947 MS.gene04856:CDS 55.0%
CCCCCATAGTAGTCAGTGAG+GGG + chr7.2:16656678-16656697 None:intergenic 55.0%
CCCTCCAACTCAACCATGAC+AGG + chr7.2:16656854-16656873 None:intergenic 55.0%
GCCACCAGAGCCTGAAATTG+AGG - chr7.2:16655199-16655218 MS.gene04856:intron 55.0%
GTACCCGCGCTCTTCAAATC+AGG - chr7.2:16655446-16655465 MS.gene04856:intron 55.0%
GTCGCTACTTGCCTGATGGA+AGG - chr7.2:16657970-16657989 MS.gene04856:intron 55.0%
TCCTGGAAGAGACAAGTGGG+TGG - chr7.2:16658149-16658168 MS.gene04856:CDS 55.0%
! CCCCTCACTGACTACTATGG+GGG - chr7.2:16656675-16656694 MS.gene04856:intron 55.0%
!! GTCCTTCTGGGACGTTCAAC+CGG + chr7.2:16655385-16655404 None:intergenic 55.0%
CCGCTCTCGTAGCTCCTTCA+TGG + chr7.2:16657931-16657950 None:intergenic 60.0%
CTCCGGTTGAACGTCCCAGA+AGG - chr7.2:16655380-16655399 MS.gene04856:intron 60.0%
GGGGGAAACTGACCCACTTG+AGG - chr7.2:16656871-16656890 MS.gene04856:intron 60.0%
! AGAAAGAGGCTGGCCATCGG+TGG + chr7.2:16655661-16655680 None:intergenic 60.0%
! AGGCTGGCCATCGGTGGTTA+GGG + chr7.2:16655655-16655674 None:intergenic 60.0%
! GCATGTGCAGGAAGGTGCAG+AGG - chr7.2:16655279-16655298 MS.gene04856:CDS 60.0%
! TGTGCAGGAAGGTGCAGAGG+AGG - chr7.2:16655282-16655301 MS.gene04856:CDS 60.0%
! GAGGCTGGCCATCGGTGGTT+AGG + chr7.2:16655656-16655675 None:intergenic 65.0%
! GCAGGAAGGTGCAGAGGAGG+AGG - chr7.2:16655285-16655304 MS.gene04856:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 16655064 16658178 16655064 ID=MS.gene04856
chr7.2 mRNA 16655064 16658178 16655064 ID=MS.gene04856.t1;Parent=MS.gene04856
chr7.2 exon 16658144 16658178 16658144 ID=MS.gene04856.t1.exon1;Parent=MS.gene04856.t1
chr7.2 CDS 16658144 16658178 16658144 ID=cds.MS.gene04856.t1;Parent=MS.gene04856.t1
chr7.2 exon 16658022 16658055 16658022 ID=MS.gene04856.t1.exon2;Parent=MS.gene04856.t1
chr7.2 CDS 16658022 16658055 16658022 ID=cds.MS.gene04856.t1;Parent=MS.gene04856.t1
chr7.2 exon 16657775 16657953 16657775 ID=MS.gene04856.t1.exon3;Parent=MS.gene04856.t1
chr7.2 CDS 16657775 16657953 16657775 ID=cds.MS.gene04856.t1;Parent=MS.gene04856.t1
chr7.2 exon 16656350 16656401 16656350 ID=MS.gene04856.t1.exon4;Parent=MS.gene04856.t1
chr7.2 CDS 16656350 16656401 16656350 ID=cds.MS.gene04856.t1;Parent=MS.gene04856.t1
chr7.2 exon 16655251 16655318 16655251 ID=MS.gene04856.t1.exon5;Parent=MS.gene04856.t1
chr7.2 CDS 16655251 16655318 16655251 ID=cds.MS.gene04856.t1;Parent=MS.gene04856.t1
chr7.2 exon 16655064 16655133 16655064 ID=MS.gene04856.t1.exon6;Parent=MS.gene04856.t1
chr7.2 CDS 16655064 16655133 16655064 ID=cds.MS.gene04856.t1;Parent=MS.gene04856.t1
Gene Sequence

>MS.gene04856

ATGGCCGACGATGACTACGAAGATATCGACATGGGGTTCTCTTTCTCTTTCTCTCTCTCACTCACATAATTAATAATTAATTAGGGTTTGTATTATTTTTTACTCATGATTAAAACGATGCAGGTACGAGGAGGAGCCACCAGAGCCTGAAATTGAGGTACCATACTTCTTCTTTTTTTCTTTAATTAGGGTTTATGAAATTGTTGATGATTGATGCATGTGCAGGAAGGTGCAGAGGAGGAGGATCCTGATAACAAGAATGATGACCTAGATGCAGAAGGAGAACCAATTGAAACTGAGGAAAAGGAAGATGCTGCTCCGGTTGAACGTCCCAGAAGGACTTCCAAGTATATGACTAAGTATGAGCGTGCTAGGATTCTCGGTACCCGCGCTCTTCAAATCAGGTTTACAGCTTAATTTCATCACCCTATGTATGATTTTGCTTATTCATTAGTATTTGTGTTAGCTATGGAATCTTGATCAATGGGTTATGTAAAATGGGACAAGCACGAGCTTCCTTCCAGTTTGTCCTGTTTTACATCATCCATTGATCAAGGTAACCAAAAGGCAAGCAAGAGCAATGTGTATCCCTAACCACCGATGGCCAGCCTCTTTCTCATCCTTACTCTATTTAGAACATACAATTAAATTAAATTGAACTATGTTAACATGGGTCTGCGCTTAACATATACTTGTGTAATAGGAACTCTACTAACAGTATTCTTGTATTTCCTCCACGAATTTTCGTTGGATGTCTAAGAACAAATTTGTCAACCATCCTGAGTTTGTCTCTAGGTTGTGAAACGCAGGGGAGAGAGAGAGAGACAATTTGTTAAGAAATCTACATACGTGTCGGTTGTTGGAATATGACTGATAATTAAGTTCTTGTTGAGGCCATATTCTCCAACATAAAAGTTGTCAACTCCACTTGCATGGTTCTTTTGTGGAGGATTGCATTATGAGACAAGGATACTGCTCAAGTTGTCAAACAAAATTTGAGGAGTGCTGAAAGGAATCTGAAGTTCGTGAACATAGATCACAGCCATAGGATTTCAGCTTCTGTGTTCTGCTTCTGCCTAGATTAGAAACCACTGGAGTTTTCTTCATGGACATAGACTTACTGACATCTGAGTCAGATGCCTAGTCTGCATCACAAAAATCCAGAATAGATGCTGGTTCTGTCAGGTTTTCCGTTCTGTAGGAGTGAACCATGGTCCTTGAGTGATAGTGCCTCTAAGATACCTGAGTATTCGTTTTTACAGCTTTCTAATGCTCCTTTATTGCATGTTACATAAATTGACAAGCTTTTTGGACAACAAAATAGGTTGCAGGCCTTGTAAGGTTCATATTGCAATGCCCCAAACAACTTATCAATAGAGTAGGATCCTGCTTAGAGATTGCACCAAGCACCATACTTCAATAAAAGTTGCAATAACTAACTGCCAACTTAACCTAACTGTCAAGCTTCTTGAAAGAGAACTAATTCTCTGCACAGTGAGCTTACAAAGAATGCAAAACCCAGTACAATACACTACTATGCAATATGCATATTAAAAACAACAATGACTAAATGAAACAACAATGACTAAATGCCCGTTTGGTTTAAATTCTCCCCTCACTGACTACTATGGGGGAAAACACTTACCAAGGCAACTTTTTGACAAAAGTTACTTTTTTCTTCCGTTGAAAGAACTTTTTGTTAGCCTTTAAAATCTTAAACAATCATACTGCAGGTCTAATATGCCATAACTTGTTGTCTTTCTTGTTGTATGTGCAGTATGAATGCACCTGTCATGGTTGAGTTGGAGGGGGAAACTGACCCACTTGAGGTAATTTAACCATTTGATCTCTTTGCGATTCAATACCTTCTTTCTTGTTACAGTACTGCATTTGACTACTAATTGTGAGAATATCGTTGTCAAATTTTCAAACATATGTCAGTTGCGCTATGCCCCACTCAATTGAAATATAGAAAATGATTCATTACGAAGCTTCCTTACTTCTACTTTATTGATCTCCTTCATTAAGTAGTGTACCATGTAATGCACAATTGCACATACTCCAGATGTTGATCTTGTTTGTATGCTTAGATCATTGACTATGGTCAACTATACAGTTGTACCAATATATGCTGACATACATGTATAGTAACAAGGGTTTCATCTTCTCATATATTCATTTTCAATTAACCTATATTTTGAAGACGAGATCAAATTCCATCAGAAATAGGCTTATTTTTATATTAGGTTTAATAAATTCCTGTTATAGTTCATTTGTTCAGCTTGTAGATTGGTTTTCCAACCATTGTCCATAGCTTGTACTTTCTTGGCTCGGGATCTTTCTCCCTTTAAAGAAAAAGGTCCTGAACTTTGAAAATTATAGCATAAACTGGGTTGTTCTTCAGTTTGACAGTCGCATGATTTTTTCAATGTTCCATCGATGAAACTTTTTTTAAAAAATTATATAAGACTGTCATCATGACTTCGCTGAACTGATATCTTTGATGAAATTCTTGTCTTCTAATGTTGAATCTGACCACATGTTAATTTAGGATTTAAATATTCTCATGGTTGAGTAGTGTATCTTGTTTTATTTTTAGAGTCCTTTGGTATTGCTACATGTTATCTGATTCTCAAGCTCTTTAAATTTGTAAAGAATACATTGTGCACTCTATACTACGAATTACATGATTAAAATTTGACGATTTCTTATAGTAGTTTAGACGTTGTCTGTGTTCGATTCAGCAATATCCATATTTTTTTGTTGTGTTGGCTCTGCCATGGTTTCATTTTGTATCAGTTGCAATGCTATGCATTTTTTTTAGTGTAATGTCTGATATTTTATTTTTCCAACTCTTCAGATTGCCATGAAGGAGCTACGAGAGCGGAAAATTCCTTTTACCATCCGTCGCTACTTGCCTGATGGAAGGTAAATACTACTGTTTGTTTATTAAGTTGGGTACTTCTTTTTCTTGGATGGGAGGGTTAAGGCTGATACAATTCCTCCATCTTTTATGTCGTACTTACCATTTTGGCAATTGTCCAGCTATGAAGATTGGGGAGTTGATGAGCTGATTGTGGAAGACTCCTGGAAGAGACAAGTGGGTGGTGATTGA
Protein sequence

>MS.gene04856.t1

MADDDYEDIDMGYEEEPPEPEIEEGAEEEDPDNKNDDLDAEGEPIETEEKEDAAPVERPRRTSKYMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDGSYEDWGVDELIVEDSWKRQVGGD