AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34548

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34548.t1 MTR_0019s0160 97.222 144 4 0 1 144 1 144 5.60e-98 278
MS.gene34548.t1 MTR_7g092450 84.138 145 22 1 1 144 1 145 9.50e-73 214
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34548.t1 AT5G51940 79.310 145 28 2 1 144 1 144 1.04e-72 214
MS.gene34548.t1 AT2G04630 79.310 145 28 2 1 144 1 144 9.51e-72 212

Find 47 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATGAGCGTGCACGGATTT+TGG 0.198808 6.3:+53554256 MS.gene34548:CDS
TTTCTCTCGAAGCTCTTTCA+TGG 0.342410 6.3:-53555887 None:intergenic
GACTCATGGAAGAGGCAAAT+TGG 0.371145 6.3:+53556111 MS.gene34548:CDS
AATTGTGCAGCTACGAAGAT+TGG 0.389800 6.3:+53556064 MS.gene34548:intron
ATTGTGCAGCTACGAAGATT+GGG 0.412639 6.3:+53556065 MS.gene34548:intron
GCCTATTGAAACCGAGGAAA+AGG 0.427074 6.3:+53554181 MS.gene34548:CDS
GAATTCCTTACTTCAATCTC+AGG 0.430246 6.3:-53554022 None:intergenic
GTCTTTCGAGGCCTTTCTGT+TGG 0.438965 6.3:-53554215 None:intergenic
AATCCGTGCACGCTCATACT+TGG 0.443078 6.3:-53554252 None:intergenic
GTACCCGTGCTCTTCAAATC+AGG 0.451117 6.3:+53554279 MS.gene34548:CDS
GGCAAATTGGTGGTGATTGA+TGG 0.457288 6.3:+53556124 MS.gene34548:CDS
ATATATTTCGATGTCTTTCG+AGG 0.473400 6.3:-53554227 None:intergenic
ACTTCAATCTCAGGATCTAC+TGG 0.475450 6.3:-53554013 None:intergenic
GACTACAATGACTTGGACAT+CGG 0.488556 6.3:+53552876 MS.gene34548:CDS
CAAGTAACGACGGATGGTAA+AGG 0.490956 6.3:-53555917 None:intergenic
CACAGTAATTACCTTCCATC+AGG 0.492790 6.3:-53555940 None:intergenic
CCTTCCAACTCAACCATGAC+AGG 0.504355 6.3:-53554414 None:intergenic
GGGAGTTGATGAATTGATTG+TGG 0.509393 6.3:+53556086 MS.gene34548:CDS
AAGTAACGACGGATGGTAAA+GGG 0.509534 6.3:-53555916 None:intergenic
ACTACAATGACTTGGACATC+GGG 0.528904 6.3:+53552877 MS.gene34548:CDS
AAAGTTTCTCGTACCTCAAG+TGG 0.531917 6.3:-53554447 None:intergenic
ATTACCTGATTTGAAGAGCA+CGG 0.540995 6.3:-53554283 None:intergenic
CTGTCATGGTTGAGTTGGAA+GGG 0.541235 6.3:+53554415 MS.gene34548:CDS
CGACGACGACTACAATGACT+TGG 0.542431 6.3:+53552869 MS.gene34548:CDS
ATCCGTCGTTACTTGCCTGA+TGG 0.543002 6.3:+53555925 MS.gene34548:CDS
TGCACCTGTCATGGTTGAGT+TGG 0.544974 6.3:+53554410 MS.gene34548:CDS
TTACCTGATTTGAAGAGCAC+GGG 0.554617 6.3:-53554282 None:intergenic
AATTGATTGTGGAAGACTCA+TGG 0.564886 6.3:+53556097 MS.gene34548:CDS
GACAACAATGATGAGATGGC+TGG 0.565670 6.3:+53554155 MS.gene34548:CDS
AACAAGAAGAGCCAACAGAA+AGG 0.566880 6.3:+53554204 MS.gene34548:CDS
TGACCAAGTATGAGCGTGCA+CGG 0.583030 6.3:+53554249 MS.gene34548:CDS
GTCGTTACTTGCCTGATGGA+AGG 0.583890 6.3:+53555929 MS.gene34548:CDS
TTGTGGAAGACTCATGGAAG+AGG 0.587346 6.3:+53556103 MS.gene34548:CDS
CCTGTCATGGTTGAGTTGGA+AGG 0.591673 6.3:+53554414 MS.gene34548:CDS
CAAGTCATTGTAGTCGTCGT+CGG 0.596315 6.3:-53552868 None:intergenic
ATCAGGCAAGTAACGACGGA+TGG 0.598660 6.3:-53555923 None:intergenic
AAGTATGAATGCACCTGTCA+TGG 0.600527 6.3:+53554401 MS.gene34548:intron
TTGATAGGAAGGTGCAGAGG+TGG 0.602482 6.3:+53554118 MS.gene34548:intron
TGTCATGGTTGAGTTGGAAG+GGG 0.604272 6.3:+53554416 MS.gene34548:CDS
TCATGGAAGAGGCAAATTGG+TGG 0.616416 6.3:+53556114 MS.gene34548:CDS
CAACGACAACAATGATGAGA+TGG 0.626792 6.3:+53554151 MS.gene34548:CDS
TGGAGAGCCTATTGAAACCG+AGG 0.643087 6.3:+53554175 MS.gene34548:CDS
AGGGGAGACTGACCCACTTG+AGG 0.643885 6.3:+53554434 MS.gene34548:CDS
TTCCATCAGGCAAGTAACGA+CGG 0.645675 6.3:-53555927 None:intergenic
TTGTGCAGCTACGAAGATTG+GGG 0.648090 6.3:+53556066 MS.gene34548:intron
TGGTTGATAGGAAGGTGCAG+AGG 0.655672 6.3:+53554115 MS.gene34548:intron
AAGTTTCTCGTACCTCAAGT+GGG 0.679566 6.3:-53554446 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGCTAAAAAAAAAAAAAA+AGG - chr6.3:53555973-53555992 None:intergenic 10.0%
!! TCAAAAATGAAAATTAAATT+GGG - chr6.3:53552966-53552985 None:intergenic 10.0%
!! TTCAAAAATGAAAATTAAAT+TGG - chr6.3:53552967-53552986 None:intergenic 10.0%
!!! AAAATTTGTTATTATTGTAA+GGG - chr6.3:53555141-53555160 None:intergenic 10.0%
!!! AAAATTTTACAAAGAAAATT+AGG - chr6.3:53555666-53555685 None:intergenic 10.0%
!!! ATATTTTCATATTACAATAA+GGG + chr6.3:53554947-53554966 MS.gene34548:intron 10.0%
!!! TAAAATTTGTTATTATTGTA+AGG - chr6.3:53555142-53555161 None:intergenic 10.0%
!!! TGATGAATTTTTTAAATTAT+TGG + chr6.3:53554095-53554114 MS.gene34548:intron 10.0%
!!! TTTTTTTTTTTTTTTAGCTT+TGG + chr6.3:53555972-53555991 MS.gene34548:intron 10.0%
!! AAAAATAATAAAACGATATC+AGG - chr6.3:53553014-53553033 None:intergenic 15.0%
!! CAAAAATGAAAATTAAATTG+GGG - chr6.3:53552965-53552984 None:intergenic 15.0%
!! CTTATTGTAATATGAAAATA+TGG - chr6.3:53554948-53554967 None:intergenic 15.0%
!! GGAATTAATTATTATCAATA+AGG - chr6.3:53552993-53553012 None:intergenic 15.0%
!! TCAAATATCATTTATCTATA+TGG - chr6.3:53555558-53555577 None:intergenic 15.0%
!! TTTACAATCTTATTATTCAA+AGG - chr6.3:53555513-53555532 None:intergenic 15.0%
!!! CATATTTTCATATTACAATA+AGG + chr6.3:53554946-53554965 MS.gene34548:intron 15.0%
!!! TTTTTAAATTATTGGTTGAT+AGG + chr6.3:53554103-53554122 MS.gene34548:intron 15.0%
!! ACAAAACATAAAAATGAACA+TGG - chr6.3:53553068-53553087 None:intergenic 20.0%
!! ACATTAGCAGTAAAAAAAAA+GGG - chr6.3:53554899-53554918 None:intergenic 20.0%
!! AGCTAAACATACAAAATTAT+AGG - chr6.3:53555238-53555257 None:intergenic 20.0%
!! ATATACAAATATACACCAAA+TGG + chr6.3:53554821-53554840 MS.gene34548:intron 20.0%
!! ATCATGTTAAAACATTTACA+AGG - chr6.3:53554361-53554380 None:intergenic 20.0%
!! ATCATTTATCTATATGGTAT+TGG - chr6.3:53555552-53555571 None:intergenic 20.0%
!! CCAAACATATATGAATATTA+CGG + chr6.3:53554062-53554081 MS.gene34548:intron 20.0%
!! CGTAATATTCATATATGTTT+GGG - chr6.3:53554064-53554083 None:intergenic 20.0%
!! GCTTAAAAACATGAATATAT+AGG - chr6.3:53553133-53553152 None:intergenic 20.0%
!! TACATTAGCAGTAAAAAAAA+AGG - chr6.3:53554900-53554919 None:intergenic 20.0%
!! TATAGGAAGAAAAAACAAAA+AGG - chr6.3:53553116-53553135 None:intergenic 20.0%
!! TCTAATTTCAAAACATATGT+CGG + chr6.3:53554615-53554634 MS.gene34548:intron 20.0%
!! TTACAAAGAAAATTAGGTAT+TGG - chr6.3:53555660-53555679 None:intergenic 20.0%
!!! AAAAAGGTTACATTTTACTT+TGG - chr6.3:53553100-53553119 None:intergenic 20.0%
!!! AATCTTTAAAGACATGTATA+TGG - chr6.3:53553587-53553606 None:intergenic 20.0%
!!! TAAATATGTTTTGAAGTCAA+CGG - chr6.3:53555458-53555477 None:intergenic 20.0%
!!! TAACAAATTTTAATATGGGA+AGG + chr6.3:53555150-53555169 MS.gene34548:intron 20.0%
!!! TTTATTTTGAAATATGGATC+AGG - chr6.3:53554800-53554819 None:intergenic 20.0%
!!! TTTTTTTACTGCTAATGTAT+GGG + chr6.3:53554901-53554920 MS.gene34548:intron 20.0%
!!! TTTTTTTTACTGCTAATGTA+TGG + chr6.3:53554900-53554919 MS.gene34548:intron 20.0%
! AAAAGATCAGACAGTTTAAA+AGG + chr6.3:53553872-53553891 MS.gene34548:intron 25.0%
! AAACTTAAACATTTACTTCG+TGG + chr6.3:53555341-53555360 MS.gene34548:intron 25.0%
! ATAAGCCTTTATCATGATAA+AGG + chr6.3:53553511-53553530 MS.gene34548:intron 25.0%
! ATAAGCCTTTATCATGATAA+AGG - chr6.3:53553530-53553511 None:intergenic 25.0%
! CCATAAAAAGTTACCATTTA+AGG - chr6.3:53555010-53555029 None:intergenic 25.0%
! CCGTAATATTCATATATGTT+TGG - chr6.3:53554065-53554084 None:intergenic 25.0%
! TCTCTCATAACAAATTTGAA+TGG + chr6.3:53553969-53553988 MS.gene34548:intron 25.0%
! TTGAATGTAATAACATGTGT+CGG + chr6.3:53553747-53553766 MS.gene34548:intron 25.0%
!! CCTTAAATGGTAACTTTTTA+TGG + chr6.3:53555007-53555026 MS.gene34548:intron 25.0%
!! GCTTATTTGAGTTTATCTAA+TGG + chr6.3:53553183-53553202 MS.gene34548:intron 25.0%
!! GGAAAAATTTGTTCTTTCAA+AGG - chr6.3:53553849-53553868 None:intergenic 25.0%
!! GTGAAGTTAAAATTTTGTTC+TGG + chr6.3:53554326-53554345 MS.gene34548:intron 25.0%
!! TACATGTCTTTAAAGATTGA+AGG + chr6.3:53553589-53553608 MS.gene34548:intron 25.0%
!! TGTAACCATTTGATACATTT+TGG + chr6.3:53555822-53555841 MS.gene34548:intron 25.0%
!! TTGTAAATTTCTCCTTTTGA+TGG + chr6.3:53555526-53555545 MS.gene34548:intron 25.0%
!!! TAAACTGTCTGATCTTTTTT+AGG - chr6.3:53553870-53553889 None:intergenic 25.0%
!!! TAAATTATTGGTTGATAGGA+AGG + chr6.3:53554107-53554126 MS.gene34548:intron 25.0%
!!! TTAAGCTTGTTTTGCATTAT+GGG + chr6.3:53553147-53553166 MS.gene34548:intron 25.0%
!!! TTTAAGCTTGTTTTGCATTA+TGG + chr6.3:53553146-53553165 MS.gene34548:intron 25.0%
!!! TTTTTTTTTGTAAGTTCTCC+AGG + chr6.3:53553415-53553434 MS.gene34548:intron 25.0%
AAAACACATATCTCATGAAC+TGG - chr6.3:53553925-53553944 None:intergenic 30.0%
ACAAATATACACCAAATGGT+GGG + chr6.3:53554825-53554844 MS.gene34548:intron 30.0%
AGATACTAGCATAACAAGTT+AGG - chr6.3:53554558-53554577 None:intergenic 30.0%
ATATATTTCGATGTCTTTCG+AGG - chr6.3:53554230-53554249 None:intergenic 30.0%
ATCAGACAGTTTAAAAGGAA+TGG + chr6.3:53553877-53553896 MS.gene34548:intron 30.0%
CATTATGGGTATGTTTGAAT+TGG + chr6.3:53553161-53553180 MS.gene34548:intron 30.0%
CTGGTTACTTCTATTATGTT+TGG + chr6.3:53555431-53555450 MS.gene34548:intron 30.0%
GAGAGAAAATTGACGAAAAA+TGG - chr6.3:53555585-53555604 None:intergenic 30.0%
TAATGTTGATCGAAAAGAAC+TGG + chr6.3:53555412-53555431 MS.gene34548:intron 30.0%
TACAAATATACACCAAATGG+TGG + chr6.3:53554824-53554843 MS.gene34548:intron 30.0%
TGTGTCCAAAATGTATCAAA+TGG - chr6.3:53555830-53555849 None:intergenic 30.0%
TTAAACTGTTTAGCCAAACA+TGG + chr6.3:53553554-53553573 MS.gene34548:intron 30.0%
TTCATACTTCATCAACCAAA+AGG - chr6.3:53554390-53554409 None:intergenic 30.0%
TTGAACATAATCACATGTGT+TGG + chr6.3:53553274-53553293 MS.gene34548:intron 30.0%
! GTTTTGTAGTATGTTACTAG+TGG + chr6.3:53553940-53553959 MS.gene34548:intron 30.0%
!! TCAAATTCTTTAGCTTGCAT+TGG + chr6.3:53555781-53555800 MS.gene34548:intron 30.0%
!!! ACAGAGGTTTTAAGACTTTT+TGG + chr6.3:53553331-53553350 MS.gene34548:intron 30.0%
AAAGTGAATGATCGAAGTTG+GGG - chr6.3:53554692-53554711 None:intergenic 35.0%
AATTGATTGTGGAAGACTCA+TGG + chr6.3:53556097-53556116 MS.gene34548:CDS 35.0%
AGACAGTTTAAAAGGAATGG+AGG + chr6.3:53553880-53553899 MS.gene34548:intron 35.0%
AGTAAAAAAAAAGGGCAACC+CGG - chr6.3:53554891-53554910 None:intergenic 35.0%
AGTATGTGTAATCAGTGGAT+TGG + chr6.3:53555031-53555050 MS.gene34548:intron 35.0%
ATATGGACTTAGACCATGTT+TGG - chr6.3:53553570-53553589 None:intergenic 35.0%
ATTACCACACTCACCAAATT+AGG + chr6.3:53552933-53552952 MS.gene34548:intron 35.0%
ATTGGGGAAAAACCCTAATT+TGG - chr6.3:53552949-53552968 None:intergenic 35.0%
CAAAGTGAATGATCGAAGTT+GGG - chr6.3:53554693-53554712 None:intergenic 35.0%
CACTAGTTAAGGACCTTAAA+TGG + chr6.3:53554994-53555013 MS.gene34548:intron 35.0%
GAATTCCTTACTTCAATCTC+AGG - chr6.3:53554025-53554044 None:intergenic 35.0%
GACAGTTTAAAAGGAATGGA+GGG + chr6.3:53553881-53553900 MS.gene34548:intron 35.0%
TAGATCCTGAGATTGAAGTA+AGG + chr6.3:53554017-53554036 MS.gene34548:intron 35.0%
TGCATTTGAGTATGTCTGAA+GGG + chr6.3:53554513-53554532 MS.gene34548:intron 35.0%
TTACCACACTCACCAAATTA+GGG + chr6.3:53552934-53552953 MS.gene34548:intron 35.0%
! ACTTTCTCTCTCTTTTGCTT+TGG + chr6.3:53555626-53555645 MS.gene34548:intron 35.0%
! ATAATCACATGTGTTGGTGT+TGG + chr6.3:53553280-53553299 MS.gene34548:intron 35.0%
! ATGGAAGTATGTGTAATCAG+TGG + chr6.3:53555026-53555045 MS.gene34548:intron 35.0%
! ATTACCTGATTTGAAGAGCA+CGG - chr6.3:53554286-53554305 None:intergenic 35.0%
! CTGGAACCTTTTCATTTACA+GGG - chr6.3:53555308-53555327 None:intergenic 35.0%
! TAACATGATGTCTTGCCTTT+TGG + chr6.3:53554372-53554391 MS.gene34548:intron 35.0%
! TGGAACCTTTTCATTTACAG+GGG - chr6.3:53555307-53555326 None:intergenic 35.0%
!! AAGTTTTAATGTCCAAAGCC+TGG - chr6.3:53555327-53555346 None:intergenic 35.0%
!! ATATGGTATTGGCCATCAAA+AGG - chr6.3:53555541-53555560 None:intergenic 35.0%
AAAGTTTCTCGTACCTCAAG+TGG - chr6.3:53554450-53554469 None:intergenic 40.0%
AACAAGAAGAGCCAACAGAA+AGG + chr6.3:53554204-53554223 MS.gene34548:CDS 40.0%
AAGTAACGACGGATGGTAAA+GGG - chr6.3:53555919-53555938 None:intergenic 40.0%
AAGTATGAATGCACCTGTCA+TGG + chr6.3:53554401-53554420 MS.gene34548:intron 40.0%
AAGTTTCTCGTACCTCAAGT+GGG - chr6.3:53554449-53554468 None:intergenic 40.0%
AATGAAAAGGTTCCAGGCTT+TGG + chr6.3:53555312-53555331 MS.gene34548:intron 40.0%
AATTGTGCAGCTACGAAGAT+TGG + chr6.3:53556064-53556083 MS.gene34548:intron 40.0%
ACAGAAAACGCATGACATGA+AGG - chr6.3:53556036-53556055 None:intergenic 40.0%
ACTTCAATCTCAGGATCTAC+TGG - chr6.3:53554016-53554035 None:intergenic 40.0%
AGTGGTCTTCATATGGAATG+GGG + chr6.3:53554646-53554665 MS.gene34548:intron 40.0%
ATAGACCAGTGGTCTTCATA+TGG + chr6.3:53554639-53554658 MS.gene34548:intron 40.0%
ATTGTGCAGCTACGAAGATT+GGG + chr6.3:53556065-53556084 MS.gene34548:intron 40.0%
CAACGACAACAATGATGAGA+TGG + chr6.3:53554151-53554170 MS.gene34548:CDS 40.0%
CACAGTAATTACCTTCCATC+AGG - chr6.3:53555943-53555962 None:intergenic 40.0%
CAGTGGTCTTCATATGGAAT+GGG + chr6.3:53554645-53554664 MS.gene34548:intron 40.0%
CCAAAGTGAATGATCGAAGT+TGG - chr6.3:53554694-53554713 None:intergenic 40.0%
CCAACTTCGATCATTCACTT+TGG + chr6.3:53554691-53554710 MS.gene34548:intron 40.0%
CCTGTAAATGAAAAGGTTCC+AGG + chr6.3:53555306-53555325 MS.gene34548:intron 40.0%
CTGCATTTGAGTATGTCTGA+AGG + chr6.3:53554512-53554531 MS.gene34548:intron 40.0%
TATTACCAGTGTAGACGTGT+CGG - chr6.3:53553235-53553254 None:intergenic 40.0%
TTTCTCTCGAAGCTCTTTCA+TGG - chr6.3:53555890-53555909 None:intergenic 40.0%
! AAACCCTAATTTGGTGAGTG+TGG - chr6.3:53552940-53552959 None:intergenic 40.0%
! ACTACAATGACTTGGACATC+GGG + chr6.3:53552877-53552896 MS.gene34548:CDS 40.0%
! CCTGGAACCTTTTCATTTAC+AGG - chr6.3:53555309-53555328 None:intergenic 40.0%
! CTCTTCTTGTTCCTTTTCCT+CGG - chr6.3:53554195-53554214 None:intergenic 40.0%
! GACTACAATGACTTGGACAT+CGG + chr6.3:53552876-53552895 MS.gene34548:CDS 40.0%
! TCCTTTTCCTCGGTTTCAAT+AGG - chr6.3:53554185-53554204 None:intergenic 40.0%
! TTACCTGATTTGAAGAGCAC+GGG - chr6.3:53554285-53554304 None:intergenic 40.0%
! TTTTGTAAGTTCTCCAGGAC+AGG + chr6.3:53553420-53553439 MS.gene34548:intron 40.0%
!! GCTATTTCTATAACCTGTCC+TGG - chr6.3:53553436-53553455 None:intergenic 40.0%
!! GGGAGTTGATGAATTGATTG+TGG + chr6.3:53556086-53556105 MS.gene34548:CDS 40.0%
!! TAGCTTTGGAAACGAGTGAA+AGG + chr6.3:53555986-53556005 MS.gene34548:intron 40.0%
AAATGTTAACTAGTGCCCCC+GGG + chr6.3:53554970-53554989 MS.gene34548:intron 45.0%
AAGGACTCCGTTAACTAGGA+AGG - chr6.3:53554724-53554743 None:intergenic 45.0%
ACTCCGTTAACTAGGAAGGA+GGG - chr6.3:53554720-53554739 None:intergenic 45.0%
AGTCAAGGACTCCGTTAACT+AGG - chr6.3:53554728-53554747 None:intergenic 45.0%
AGTTCATCTTTCCTTTGCCC+TGG + chr6.3:53555281-53555300 MS.gene34548:intron 45.0%
CAAGTAACGACGGATGGTAA+AGG - chr6.3:53555920-53555939 None:intergenic 45.0%
CAAGTCATTGTAGTCGTCGT+CGG - chr6.3:53552871-53552890 None:intergenic 45.0%
CACAGCCATAGTTAGAGTCA+AGG - chr6.3:53554743-53554762 None:intergenic 45.0%
CATATGTCGGTATAGACCAG+TGG + chr6.3:53554628-53554647 MS.gene34548:intron 45.0%
CCAGTGGTCTTCATATGGAA+TGG + chr6.3:53554644-53554663 MS.gene34548:intron 45.0%
CCATTCCATATGAAGACCAC+TGG - chr6.3:53554647-53554666 None:intergenic 45.0%
CTTGACTCTAACTATGGCTG+TGG + chr6.3:53554741-53554760 MS.gene34548:intron 45.0%
GAAATGTTAACTAGTGCCCC+CGG + chr6.3:53554969-53554988 MS.gene34548:intron 45.0%
GACAACAATGATGAGATGGC+TGG + chr6.3:53554155-53554174 MS.gene34548:CDS 45.0%
GACTCATGGAAGAGGCAAAT+TGG + chr6.3:53556111-53556130 MS.gene34548:CDS 45.0%
GCCTATTGAAACCGAGGAAA+AGG + chr6.3:53554181-53554200 MS.gene34548:CDS 45.0%
GGAGTCCTTGACTCTAACTA+TGG + chr6.3:53554735-53554754 MS.gene34548:intron 45.0%
GTGGTCTTCATATGGAATGG+GGG + chr6.3:53554647-53554666 MS.gene34548:intron 45.0%
TACTACCAGTGTCAACGTGT+CGG - chr6.3:53553704-53553723 None:intergenic 45.0%
TCATGGAAGAGGCAAATTGG+TGG + chr6.3:53556114-53556133 MS.gene34548:CDS 45.0%
TGACCCTCCTTCCTAGTTAA+CGG + chr6.3:53554714-53554733 MS.gene34548:intron 45.0%
TGGTCTTCATATGGAATGGG+GGG + chr6.3:53554648-53554667 MS.gene34548:intron 45.0%
TGTCAGTGTTGTGTCTGTGT+CGG - chr6.3:53553681-53553700 None:intergenic 45.0%
TTCCATCAGGCAAGTAACGA+CGG - chr6.3:53555930-53555949 None:intergenic 45.0%
TTGTGCAGCTACGAAGATTG+GGG + chr6.3:53556066-53556085 MS.gene34548:intron 45.0%
TTGTGGAAGACTCATGGAAG+AGG + chr6.3:53556103-53556122 MS.gene34548:CDS 45.0%
! CTTTTCATTTACAGGGGCCA+GGG - chr6.3:53555301-53555320 None:intergenic 45.0%
! TATGAGCGTGCACGGATTTT+GGG + chr6.3:53554257-53554276 MS.gene34548:CDS 45.0%
!! AGGCTGATTTTCTTGTGAGG+CGG + chr6.3:53555170-53555189 MS.gene34548:intron 45.0%
!! AGTATGCTTAAAGAGTGCCC+CGG + chr6.3:53555097-53555116 MS.gene34548:intron 45.0%
!! CTGTCATGGTTGAGTTGGAA+GGG + chr6.3:53554415-53554434 MS.gene34548:CDS 45.0%
!! GGAAGGCTGATTTTCTTGTG+AGG + chr6.3:53555167-53555186 MS.gene34548:intron 45.0%
!! TGTCATGGTTGAGTTGGAAG+GGG + chr6.3:53554416-53554435 MS.gene34548:CDS 45.0%
!! AAAATAATTAATAATCAATT+TGG - chr6.3:53555753-53555772 None:intergenic 5.0%
!!! AATAATAACAAATTTTAATA+TGG + chr6.3:53555145-53555164 MS.gene34548:intron 5.0%
!!! ATAATAACAAATTTTAATAT+GGG + chr6.3:53555146-53555165 MS.gene34548:intron 5.0%
!!! TATATTTTTATTTTGAAATA+TGG - chr6.3:53554806-53554825 None:intergenic 5.0%
AAGGTCCTTAACTAGTGCCC+CGG - chr6.3:53554991-53555010 None:intergenic 50.0%
AATCCGTGCACGCTCATACT+TGG - chr6.3:53554255-53554274 None:intergenic 50.0%
AATGTTAACTAGTGCCCCCG+GGG + chr6.3:53554971-53554990 MS.gene34548:intron 50.0%
ACTAAAGCTCCCGCATATGC+AGG - chr6.3:53554865-53554884 None:intergenic 50.0%
ATCAGGCAAGTAACGACGGA+TGG - chr6.3:53555926-53555945 None:intergenic 50.0%
ATCCGTCGTTACTTGCCTGA+TGG + chr6.3:53555925-53555944 MS.gene34548:CDS 50.0%
CACCAACACCTCTGATTGTC+CGG - chr6.3:53553309-53553328 None:intergenic 50.0%
CCTGGCCCCTGTAAATGAAA+AGG + chr6.3:53555299-53555318 MS.gene34548:intron 50.0%
CCTTCCAACTCAACCATGAC+AGG - chr6.3:53554417-53554436 None:intergenic 50.0%
CGACGACGACTACAATGACT+TGG + chr6.3:53552869-53552888 MS.gene34548:CDS 50.0%
CTAAAGCTCCCGCATATGCA+GGG - chr6.3:53554864-53554883 None:intergenic 50.0%
GACTCCGTTAACTAGGAAGG+AGG - chr6.3:53554721-53554740 None:intergenic 50.0%
GGCGGGGTTGTCTTTAAATC+AGG + chr6.3:53555188-53555207 MS.gene34548:intron 50.0%
GTACCCGTGCTCTTCAAATC+AGG + chr6.3:53554279-53554298 MS.gene34548:CDS 50.0%
GTCGTTACTTGCCTGATGGA+AGG + chr6.3:53555929-53555948 MS.gene34548:CDS 50.0%
GTCTTTCGAGGCCTTTCTGT+TGG - chr6.3:53554218-53554237 None:intergenic 50.0%
TCTTGTTAACAAGTGCCCCC+GGG - chr6.3:53555118-53555137 None:intergenic 50.0%
TGACCAAGTATGAGCGTGCA+CGG + chr6.3:53554249-53554268 MS.gene34548:CDS 50.0%
TGCACCTGTCATGGTTGAGT+TGG + chr6.3:53554410-53554429 MS.gene34548:CDS 50.0%
TGGAGAGCCTATTGAAACCG+AGG + chr6.3:53554175-53554194 MS.gene34548:CDS 50.0%
! CCTTTTCATTTACAGGGGCC+AGG - chr6.3:53555302-53555321 None:intergenic 50.0%
! GCACTGACACATCTGATCGA+AGG - chr6.3:53553819-53553838 None:intergenic 50.0%
! GTATGAGCGTGCACGGATTT+TGG + chr6.3:53554256-53554275 MS.gene34548:CDS 50.0%
! GTCTTGTTAACAAGTGCCCC+CGG - chr6.3:53555119-53555138 None:intergenic 50.0%
! TAGACGTGTCGGTGCTTCAT+AGG - chr6.3:53553224-53553243 None:intergenic 50.0%
! TTGATAGGAAGGTGCAGAGG+TGG + chr6.3:53554118-53554137 MS.gene34548:intron 50.0%
!! AACATGTGTCGGTGTCGTGT+TGG + chr6.3:53553758-53553777 MS.gene34548:intron 50.0%
!! CATGTGTTGGTGTTGGACAC+CGG + chr6.3:53553287-53553306 MS.gene34548:intron 50.0%
!! CCTGTCATGGTTGAGTTGGA+AGG + chr6.3:53554414-53554433 MS.gene34548:CDS 50.0%
!! GCTGATTTTCTTGTGAGGCG+GGG + chr6.3:53555172-53555191 MS.gene34548:intron 50.0%
!! GGCTGATTTTCTTGTGAGGC+GGG + chr6.3:53555171-53555190 MS.gene34548:intron 50.0%
!! GTATGCTTAAAGAGTGCCCC+GGG + chr6.3:53555098-53555117 MS.gene34548:intron 50.0%
!! TATGCTTAAAGAGTGCCCCG+GGG + chr6.3:53555099-53555118 MS.gene34548:intron 50.0%
!! TGGTTGATAGGAAGGTGCAG+AGG + chr6.3:53554115-53554134 MS.gene34548:intron 50.0%
!! TGTCGGTGTCGTGTTGGTAT+CGG + chr6.3:53553764-53553783 MS.gene34548:intron 50.0%
AGGTCCTTAACTAGTGCCCC+GGG - chr6.3:53554990-53555009 None:intergenic 55.0%
ATTTACAGGGGCCAGGGCAA+AGG - chr6.3:53555295-53555314 None:intergenic 55.0%
CTTGTTAACAAGTGCCCCCG+GGG - chr6.3:53555117-53555136 None:intergenic 55.0%
GTTGGACACCGGACAATCAG+AGG + chr6.3:53553298-53553317 MS.gene34548:intron 55.0%
TGACACCGACACGTTGACAC+TGG + chr6.3:53553696-53553715 MS.gene34548:intron 55.0%
! ATGCGGGAGCTTTAGTGCAC+CGG + chr6.3:53554869-53554888 MS.gene34548:intron 55.0%
! CACCGGACAATCAGAGGTGT+TGG + chr6.3:53553304-53553323 MS.gene34548:intron 55.0%
! CATCTGATCGAAGGCGTGTC+TGG - chr6.3:53553810-53553829 None:intergenic 55.0%
!! AAGCACCGACACGTCTACAC+TGG + chr6.3:53553227-53553246 MS.gene34548:intron 55.0%
!! ATGCTTAAAGAGTGCCCCGG+GGG + chr6.3:53555100-53555119 MS.gene34548:intron 55.0%
!! CAGAGGTGTTGGTGCTACAG+AGG + chr6.3:53553315-53553334 MS.gene34548:intron 55.0%
AGGGGAGACTGACCCACTTG+AGG + chr6.3:53554434-53554453 MS.gene34548:CDS 60.0%
CATATGCAGGGTCCGGGAAG+GGG - chr6.3:53554852-53554871 None:intergenic 60.0%
CTTCCCGGACCCTGCATATG+CGG + chr6.3:53554852-53554871 MS.gene34548:intron 60.0%
GCATATGCAGGGTCCGGGAA+GGG - chr6.3:53554853-53554872 None:intergenic 60.0%
GGTCCTTAACTAGTGCCCCG+GGG - chr6.3:53554989-53555008 None:intergenic 60.0%
GTCCTTAACTAGTGCCCCGG+GGG - chr6.3:53554988-53555007 None:intergenic 60.0%
TTCCCGGACCCTGCATATGC+GGG + chr6.3:53554853-53554872 MS.gene34548:intron 60.0%
! CAAATGGTGGGACCCCTTCC+CGG + chr6.3:53554837-53554856 MS.gene34548:intron 60.0%
! TGCGGGAGCTTTAGTGCACC+GGG + chr6.3:53554870-53554889 MS.gene34548:intron 60.0%
CGCATATGCAGGGTCCGGGA+AGG - chr6.3:53554854-53554873 None:intergenic 65.0%
CGGGAAGGGGTCCCACCATT+TGG - chr6.3:53554839-53554858 None:intergenic 65.0%
CTCCCGCATATGCAGGGTCC+GGG - chr6.3:53554858-53554877 None:intergenic 65.0%
GCTCCCGCATATGCAGGGTC+CGG - chr6.3:53554859-53554878 None:intergenic 65.0%
! TGCCCCCGGGGCACTAGTTA+AGG + chr6.3:53554983-53555002 MS.gene34548:intron 65.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 53552864 53556143 53552864 ID=MS.gene34548
chr6.3 mRNA 53552864 53556143 53552864 ID=MS.gene34548.t1;Parent=MS.gene34548
chr6.3 exon 53552864 53552898 53552864 ID=MS.gene34548.t1.exon1;Parent=MS.gene34548.t1
chr6.3 CDS 53552864 53552898 53552864 ID=cds.MS.gene34548.t1;Parent=MS.gene34548.t1
chr6.3 exon 53554000 53554033 53554000 ID=MS.gene34548.t1.exon2;Parent=MS.gene34548.t1
chr6.3 CDS 53554000 53554033 53554000 ID=cds.MS.gene34548.t1;Parent=MS.gene34548.t1
chr6.3 exon 53554125 53554300 53554125 ID=MS.gene34548.t1.exon3;Parent=MS.gene34548.t1
chr6.3 CDS 53554125 53554300 53554125 ID=cds.MS.gene34548.t1;Parent=MS.gene34548.t1
chr6.3 exon 53554404 53554455 53554404 ID=MS.gene34548.t1.exon4;Parent=MS.gene34548.t1
chr6.3 CDS 53554404 53554455 53554404 ID=cds.MS.gene34548.t1;Parent=MS.gene34548.t1
chr6.3 exon 53555883 53555950 53555883 ID=MS.gene34548.t1.exon5;Parent=MS.gene34548.t1
chr6.3 CDS 53555883 53555950 53555883 ID=cds.MS.gene34548.t1;Parent=MS.gene34548.t1
chr6.3 exon 53556074 53556143 53556074 ID=MS.gene34548.t1.exon6;Parent=MS.gene34548.t1
chr6.3 CDS 53556074 53556143 53556074 ID=cds.MS.gene34548.t1;Parent=MS.gene34548.t1
Gene Sequence

>MS.gene34548

ATGGCCGACGACGACTACAATGACTTGGACATCGGGTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCATTACCACACTCACCAAATTAGGGTTTTTCCCCAATTTAATTTTCATTTTTGAAACACCTTATTGATAATAATTAATTCCTGATATCGTTTTATTATTTTTGATTAATTGTAGCTACAAATGTCTTGTTTAGCCATGTTCATTTTTATGTTTTGTTTGTGAAAACCAAAGTAAAATGTAACCTTTTTGTTTTTTCTTCCTATATATTCATGTTTTTAAGCTTGTTTTGCATTATGGGTATGTTTGAATTGGCTTATTTGAGTTTATCTAATGGCATAAGCAGTTGTGACCTATGAAGCACCGACACGTCTACACTGGTAATAATATGAGTAAATGAATTATTTGAACATAATCACATGTGTTGGTGTTGGACACCGGACAATCAGAGGTGTTGGTGCTACAGAGGTTTTAAGACTTTTTGGAAGAACTTATGAAAATAGCTTATGACATAATGATAAGCTATTTTCAGTTTTTTTTTTTTTTTTTTTTTTTGTAAGTTCTCCAGGACAGGTTATAGAAATAGCTTATACAAAAATAATATAACATCATTTTATTTTTTGTTATAGAAATAACTTATACATAAGCCTTTATCATGATAAAGGCTTATGTTATAAGCTGCAAATTAAACTGTTTAGCCAAACATGGTCTAAGTCCATATACATGTCTTTAAAGATTGAAGGTTGTCATGCGCTAAGTTTATTTCAAATTTACACTTAGAATGTAATTCACATATGAACTATGAAGCACCGACACAGACACAACACTGACACCGACACGTTGACACTGGTAGTAATTTGAAAAAAAAAATGACAGAATTGAATGTAATAACATGTGTCGGTGTCGTGTTGGTATCGGACACAGACACGTGTCTGACACCAGACACGCCTTCGATCAGATGTGTCAGTGCTACAAAGCCTTTGAAAGAACAAATTTTTCCTAAAAAAGATCAGACAGTTTAAAAGGAATGGAGGGAGTATGATTATGCAGTTGCCAGTTCATGAGATATGTGTTTTGTAGTATGTTACTAGTGGAACATTTCTCTCATAACAAATTTGAATGGTGATGCAGATATGAAGATGAGCCAGTAGATCCTGAGATTGAAGTAAGGAATTCTATACACACTGACTAACCCAAACATATATGAATATTACGGAATACACTGTTGATGAATTTTTTAAATTATTGGTTGATAGGAAGGTGCAGAGGTGGATGCAGATGACAACGACAACAATGATGAGATGGCTGGAGAGCCTATTGAAACCGAGGAAAAGGAACAAGAAGAGCCAACAGAAAGGCCTCGAAAGACATCGAAATATATGACCAAGTATGAGCGTGCACGGATTTTGGGTACCCGTGCTCTTCAAATCAGGTAATTTTTTTTAGTTTTGATCTTTGTGAAGTTAAAATTTTGTTCTGGTGAATGATTCCTTGTAAATGTTTTAACATGATGTCTTGCCTTTTGGTTGATGAAGTATGAATGCACCTGTCATGGTTGAGTTGGAAGGGGAGACTGACCCACTTGAGGTACGAGAAACTTTTCTTCTGTTAACTCATTTGATGTTTCATTTCACATAGTGTTGCTGCATTTGAGTATGTCTGAAGGGTTATTGAAAATTGTGCTTGCCTAACTTGTTATGCTAGTATCTAATTTCTTTTGTTTTTTTAACAAAAACTAGTATCTGATCTAATTTCAAAACATATGTCGGTATAGACCAGTGGTCTTCATATGGAATGGGGGGAGATTTCTTTAAGACACTCCCCAACTTCGATCATTCACTTTGGTGACCCTCCTTCCTAGTTAACGGAGTCCTTGACTCTAACTATGGCTGTGGAGATTTTATTTCTCTTTAGTTTCTTATCAGTTACCTGATCCATATTTCAAAATAAAAATATACAAATATACACCAAATGGTGGGACCCCTTCCCGGACCCTGCATATGCGGGAGCTTTAGTGCACCGGGTTGCCCTTTTTTTTTACTGCTAATGTATGGGCTATTCGTATGAAATCTCTTTCCATATTTTCATATTACAATAAGGGAAATGTTAACTAGTGCCCCCGGGGCACTAGTTAAGGACCTTAAATGGTAACTTTTTATGGAAGTATGTGTAATCAGTGGATTGGAAATTGAAAACTTTGACATTTTTAAAGAATAATTACTTAATTTAGTATGCTTAAAGAGTGCCCCGGGGGCACTTGTTAACAAGACCCTTACAATAATAACAAATTTTAATATGGGAAGGCTGATTTTCTTGTGAGGCGGGGTTGTCTTTAAATCAGGTCTTTTATCATTCTCTTATTCTTGCCTATAATTTTGTATGTTTAGCTCATAGCAAAGTTTCGTTTCTTATAGTTCATCTTTCCTTTGCCCTGGCCCCTGTAAATGAAAAGGTTCCAGGCTTTGGACATTAAAACTTAAACATTTACTTCGTGGTTCATTGAAGAATTGATGCTCTTTCTAATGTTGAAACTTAAAAGATTATAATGTTGATCGAAAAGAACTGGTTACTTCTATTATGTTTGGTCCGTTGACTTCAAAACATATTTATACTTTTGACATTGAACGTTGATTCTGATAAACCTTTGAATAATAAGATTGTAAATTTCTCCTTTTGATGGCCAATACCATATAGATAAATGATATTTGAACAACCATTTTTCGTCAATTTTCTCTCTCATGCTCACATTATCTTTTTACTTTCTCTCTCTTTTGCTTTGGTTTTTGTGCCAATACCTAATTTTCTTTGTAAAATTTTGATTGTTTCAAAAGTTGTCATATAAGTAAATGTTCAAATAACACAACTCTACTATATATCAATTCCAAATTGATTATTAATTATTTTATATAGTTTCAAATTCTTTAGCTTGCATTGGATTTATGTTCATGTATCATGTAACCATTTGATACATTTTGGACACAAATGTCTGATATCTTGTTCACTCAACTTCACAGATTGCCATGAAAGAGCTTCGAGAGAAAAAGATACCCTTTACCATCCGTCGTTACTTGCCTGATGGAAGGTAATTACTGTGTTTAATTCCTTTTTTTTTTTTTTTAGCTTTGGAAACGAGTGAAAGGTATGTTAAGATTAAGCTGAATTTTCCTTCATGTCATGCGTTTTCTGTTTTTGCAAAATTGTGCAGCTACGAAGATTGGGGAGTTGATGAATTGATTGTGGAAGACTCATGGAAGAGGCAAATTGGTGGTGATTGA
Protein sequence

>MS.gene34548.t1

MADDDYNDLDIGYEDEPVDPEIEEGAEVDADDNDNNDEMAGEPIETEEKEQEEPTERPRKTSKYMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELREKKIPFTIRRYLPDGSYEDWGVDELIVEDSWKRQIGGD