AlfalfaGEDB | MS.gene20260

M. sativa cultivar XinJiangDaYe / MS.gene20260

KEGG: K03013
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003899 GO:0005488 GO:0005575 GO:0005634 GO:0006139 GO:0006351 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0032774 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0046483 GO:0071704 GO:0090304 GO:0097159 GO:0097659 GO:0097747 GO:0110165 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576
PFAM: PF01191.22 PF03871.17

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20260.t1	XP_003611642.1	91.7	204	17	0	2	205	127	330	1.40E-98	369

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20260.t1	O81098	69.3	205	63	0	2	206	1	205	5.9e-77	288.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20260.t1	G7K3Y0	91.7	204	17	0	2	205	127	330	1.0e-98	369.0

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene014019	MS.gene20260	PPI
MS.gene014162	MS.gene20260	PPI
MS.gene022098	MS.gene20260	PPI
MS.gene07741	MS.gene20260	PPI
MS.gene20260	MS.gene063673	PPI
MS.gene20260	MS.gene34548	PPI
MS.gene20260	MS.gene55120	PPI
MS.gene20260	MS.gene38787	PPI
MS.gene20260	MS.gene023153	PPI
MS.gene20260	MS.gene67685	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20260.t1	MTR_5g016220	91.220	205	18	0	2	206	127	331	3.13e-136	385
MS.gene20260.t1	MTR_5g016220	81.890	127	22	1	1	127	1	126	2.21e-68	212
MS.gene20260.t1	MTR_5g016200	90.594	202	16	1	1	202	1	199	4.03e-132	369
MS.gene20260.t1	MTR_5g005470	84.466	206	32	0	1	206	1	206	1.12e-129	363
MS.gene20260.t1	MTR_6g042230	62.621	206	76	1	1	206	1	205	6.20e-86	253
MS.gene20260.t1	MTR_6g088010	58.454	207	84	1	1	205	1	207	1.43e-84	249
MS.gene20260.t1	MTR_6g088020	58.654	208	82	2	1	205	1	207	3.89e-81	240
MS.gene20260.t1	MTR_7g090220	40.513	195	113	2	12	205	55	247	1.49e-44	149
MS.gene20260.t1	MTR_3g058520	33.508	191	72	5	4	194	23	158	3.32e-17	75.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20260.t1	AT3G22320	69.268	205	63	0	2	206	1	205	8.19e-106	303
MS.gene20260.t1	AT5G57980	46.117	206	107	2	5	206	5	210	2.22e-60	188
MS.gene20260.t1	AT2G41340	40.394	203	118	2	4	205	17	217	5.16e-45	149
MS.gene20260.t1	AT3G57080	35.784	204	128	2	3	205	20	221	1.70e-38	132
MS.gene20260.t1	AT3G54490	32.836	201	132	2	7	206	35	233	9.36e-32	115
MS.gene20260.t1	AT2G41340	36.313	179	102	3	4	181	17	184	2.41e-29	108
MS.gene20260.t1	AT2G41340	38.650	163	97	2	4	165	17	177	6.32e-29	107
MS.gene20260.t1	AT3G16680	46.753	77	39	1	84	158	2	78	2.16e-16	71.6

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Find 55 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCCTTATACAACAAAT+CGG	0.490397	5.1:+8503675	None:intergenic
AACGGTGTATCTCTCGAGTA+AGG	0.416794	5.1:+8505346	None:intergenic
AACTTCCAAATGAAATTTGG+AGG	0.512170	5.1:+8505540	None:intergenic
AAGACATAAATCTGGTCAGA+GGG	0.592633	5.1:+8505703	None:intergenic
AAGAGTTCAAGGAGAAGTAT+GGG	0.443886	5.1:-8505777	MS.gene20260:CDS
ACATACCGATTTGTTGTATA+AGG	0.225416	5.1:-8503680	None:intergenic
ACGCCAACCTTGTCTTCCTC+CGG	0.433207	5.1:+8505676	None:intergenic
ACTGACCTGAGTTTCTTTAA+CGG	0.397368	5.1:+8505328	None:intergenic
AGAAGACCTTGTCATCAACA+AGG	0.684606	5.1:-8505740	MS.gene20260:CDS
AGAGTTCAAGGAGAAGTATG+GGG	0.574581	5.1:-8505776	MS.gene20260:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATCAATGAAAGAACTGG+AGG	+	chr5.1:8504672-8504691	None:intergenic	30.0%
AAATAGATTCAAAAGCTCTC+AGG	+	chr5.1:8505562-8505581	None:intergenic	30.0%
AAATCAATGAAAGAACTGGA+GGG	+	chr5.1:8504671-8504690	None:intergenic	30.0%
AACTAGATTGTACATCATCA+TGG	+	chr5.1:8505393-8505412	None:intergenic	30.0%
AACTTCCAAATGAAATTTGG+AGG	+	chr5.1:8504037-8504056	None:intergenic	30.0%
AGACAATATGCAAACAAGAA+CGG	+	chr5.1:8505645-8505664	None:intergenic	30.0%
AGCTGACATAAAAATTTCAG+AGG	-	chr5.1:8504778-8504797	MS.gene20260:intron	30.0%
ATGAAATAACGAAGCTGTAT+AGG	-	chr5.1:8503698-8503717	MS.gene20260:CDS	30.0%
CTAGAACAAAATCATCAAAG+AGG	+	chr5.1:8504437-8504456	None:intergenic	30.0%
CTGCTTTAATCTTTGTCTAA+TGG	-	chr5.1:8505614-8505633	MS.gene20260:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	8503682	8505914	8503682	ID=MS.gene20260
chr5.1	mRNA	8503682	8505914	8503682	ID=MS.gene20260.t1;Parent=MS.gene20260
chr5.1	exon	8505534	8505914	8505534	ID=MS.gene20260.t1.exon1;Parent=MS.gene20260.t1
chr5.1	CDS	8505534	8505914	8505534	ID=cds.MS.gene20260.t1;Parent=MS.gene20260.t1
chr5.1	exon	8505334	8505447	8505334	ID=MS.gene20260.t1.exon2;Parent=MS.gene20260.t1
chr5.1	CDS	8505334	8505447	8505334	ID=cds.MS.gene20260.t1;Parent=MS.gene20260.t1
chr5.1	exon	8503682	8503807	8503682	ID=MS.gene20260.t1.exon3;Parent=MS.gene20260.t1
chr5.1	CDS	8503682	8503807	8503682	ID=cds.MS.gene20260.t1;Parent=MS.gene20260.t1

Gene Sequence

>MS.gene20260

ATGGTGTTTTCGGAGGATGAAATAACGAAGCTGTATAGGATCCGGAAGACGGTGATGGAGATGCTTCATGACAGGAATTACCTCGTTGGAGATTTTGAGGTTAATATGTCGAAAGAAGAGTTCAAGGAGAAGTATGGGGAGAACATGAAGAGAGAAGACCTTGTCATCAACAAGGCCAAAAAAGACAATCCCTCTGACCAGATTTATGTCTTCTTCCCGGAGGAAGACAAGGTTGGCGTCCACACCTTCAGGACTTACACCAAACGCATGAACTCCGAGAATGTCTACAGGGCCATCATCGTTTGCCAGACCAGACTTACTAACCAGGCACGGAACAGTATCTCTGATATTGCCTCCAAATTTCATTTGGAAGTTTTTCAGGTAATTCATCAATCGATCAATCAATCAAGTTTTGTGGTGATTGTTAATGGTTGAGGTAACCGTTGTTAGTGTGTGCGTGCTTGCAGGAGGCTGAATTGCTCGTTAATGTAAGATATCATGTGCTTGTGCCAAAACATCAGGTGCTGACTGACAATGAGAAGAAAACCTTACTCGAGAGATACACCGTTAAAGAAACTCAGGTCAGTTATAAAAAAATTGAGGAAATTTTGTGTTATTTAGAATGTGAGTAATCGAATGAGTACACTTGATGGCATTGTAACATTGAAGGAAGGACAAAGTGAAAATGTAAACTATTAGGACTTTGTTAATGTTGTAGTAGCTGATGTTGGATTGTTATTCGTATTTTAGTTCCTCTTTGATGATTTTGTTCTAGCTTATAACATTATGTTAAATTGTTTATTTTCTTTCTTTTAGTGTTTTTGTTGATCTGGAAAATAGTGAATTAACTTCAGAATGAAGTGAGCATGAAAGAGTTTTGCTTATTCCCCTCGCTATCGGTCATCTCAAACCCCCCAACCCCTTTATCCTTTAAGACGCCACTCGGTCCACAGCCCTTTCCTATTCAACTCATGCATTATATCTCCCCCTCCAGTTCTTTCATTGATTTTCTTTGATTGTCTTAAATAATCACTTGGGTGAGTATTTAGATAGAGCTTACTGTTGCAGATTGTCAGCGGCGTACCCTGCAACATCAAGCTGACATAAAAATTTCAGAGGAAATCAGATTTTATAGTGCATAAGTCTGGAAATTAGAGCGATTTTGGTAGTACACACTAAGATTGTTGAGTGATTTAAAGCTGAATATCGTGTATAATACTATTAATTATAGAAAATTAAGTGATCTGTCTAAGCTTTTAGATGTATTCTCAGGTTAGTGGATACTGACTTTCCTAAATGACAGAGTACTTCAAATATAACTTCATCATTTGTACCTCACTCTCACTAGTCTATGATTTCGTAAAAATTAAAATATATGATGTCCTTTGCAATTAATATCACTAGATGGAACTATTCCATTAGGTGAACACCTAAAGAAAGCTAAGAAAGACAATTATTATCATATAAGGGGAAACAACATACTTATTCCAGCCATTTTCCTCTGACTATAAACAATGAATAGCTACAATCAGCCGATCACAGCAAACTGCTATAAAGCATTTCCCTTTTGGGGCATTCTCTTTACTTATCCCAGCCATTTATTTAATTTGTAAAGTTATATAATAGTATTTCACCTAGGCATTTGAAAAATTTCTGCACACTCACACGTGCTTCATCTCTGAATTTTAACCTTTCTTCTTTCCTGGTTCCATGATGATGTACAATCTAGTTCCTGATTCTAATTTAGATTTGGATGTAATTAAGAATGCATGAAAAGCAAAATTAGCATCCATTGTATTTCATGCAAATAAAACTTCTTCATTGTTTATTCCTGTAGTGAAAGTTTGTCAGATTTGTCACCGTTGTCGTTCGCTTGTCCTGAGAGCTTTTGAATCTATTTACATTGGATAGTTCTACTGGTTTTAGTGTTTACTGCTTTAATCTTTGTCTAATGGATGTACCGTTCTTGTTTGCATATTGTCTTGTATGTGCAATTGTATCATTCTACTACAAAATTGTACTGATGCTGACATGGTTCACTATATCTAATATTTGTACTGATGCTGACACGGTTCACTATATCTAATATTTATGGTCTGTTTCTCAGCTACCTAGAATCCAGGTGACGGATCCAGTTGCAAGATATTATGGCATGAAGCGTGGACAAGTTGTTAAGATAATCCGGCCAAGTGAGACTGCAGGAAGATATGTTACATACCGATTTGTTGTATAA

Protein sequence

>MS.gene20260.t1

MVFSEDEITKLYRIRKTVMEMLHDRNYLVGDFEVNMSKEEFKEKYGENMKREDLVINKAKKDNPSDQIYVFFPEEDKVGVHTFRTYTKRMNSENVYRAIIVCQTRLTNQARNSISDIASKFHLEVFQEAELLVNVRYHVLVPKHQVLTDNEKKTLLERYTVKETQLPRIQVTDPVARYYGMKRGQVVKIIRPSETAGRYVTYRFVV