AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene029745

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029745.t1 MTR_1g062770 92.611 203 8 1 1 196 1 203 4.12e-133 371
MS.gene029745.t1 MTR_3g009280 34.815 135 52 3 43 176 230 329 8.02e-13 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029745.t1 AT3G09890 58.794 199 73 3 1 190 1 199 1.73e-70 213
MS.gene029745.t1 AT3G09890 56.757 148 55 3 1 139 1 148 3.21e-44 145
MS.gene029745.t1 AT2G17390 32.836 134 56 2 43 176 226 325 2.87e-13 67.4
MS.gene029745.t1 AT4G35450 33.582 134 55 2 43 176 186 285 3.60e-13 67.0
MS.gene029745.t1 AT4G35450 33.582 134 55 2 43 176 224 323 3.71e-13 67.0
MS.gene029745.t1 AT4G35450 33.582 134 55 2 43 176 224 323 3.71e-13 67.0
MS.gene029745.t1 AT4G35450 33.582 134 55 2 43 176 224 323 3.71e-13 67.0
MS.gene029745.t1 AT4G35450 33.582 134 55 2 43 176 232 331 4.14e-13 67.0
MS.gene029745.t1 AT5G40160 30.636 173 111 3 12 176 119 290 2.83e-11 61.6
MS.gene029745.t1 AT4G19150 30.070 143 88 2 40 176 19 155 7.43e-11 59.7

Find 60 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAACCATTGCGTCTTCC+TGG 0.282234 1.2:+47765994 None:intergenic
TCCACAAAGCAAAACTTATA+TGG 0.299371 1.2:-47763443 MS.gene029745:CDS
CTTGCATCTGTGCCAGGTTC+AGG 0.300371 1.2:+47763330 None:intergenic
CTTCATCTGACCTGTTTGTA+TGG 0.360852 1.2:-47765036 MS.gene029745:CDS
ATCACATCAATGGCGGTTCC+AGG 0.366086 1.2:-47766012 None:intergenic
ATTAGATTGTTGCTGTCTAA+TGG 0.400508 1.2:-47763473 MS.gene029745:CDS
CCAGGTTCAGGTAACTCTGC+AGG 0.402815 1.2:+47763342 None:intergenic
AACGCCCTCCGCCTCGCTCT+CGG 0.412363 1.2:-47765853 MS.gene029745:intron
GGAGGAGTATCAGATTCTTC+AGG 0.441287 1.2:+47765919 None:intergenic
AGCCTCCTTGCATCTGTGCC+AGG 0.449249 1.2:+47763324 None:intergenic
GGGTACCGAGAGCGAGGCGG+AGG 0.450691 1.2:+47765848 None:intergenic
GTCACCGTGTGTGGCGGCAT+GGG 0.482621 1.2:+47765879 None:intergenic
CCTTTACACGATGCTTGTGC+AGG 0.483914 1.2:-47764852 MS.gene029745:CDS
TGATGAACCGGTGGAGGATG+GGG 0.487017 1.2:-47765067 MS.gene029745:CDS
ACTAGAAAGAGGAGCCAACT+TGG 0.490839 1.2:-47764901 MS.gene029745:CDS
GAAGAATCGAATGGTCTCTT+CGG 0.491172 1.2:-47765958 MS.gene029745:CDS
TGGTCATTTCGAATGCGCTC+AGG 0.500683 1.2:-47765016 MS.gene029745:intron
AATGGTCTCTTCGGAGAAGA+AGG 0.510479 1.2:-47765949 MS.gene029745:CDS
TTGATGAACCGGTGGAGGAT+GGG 0.512299 1.2:-47765068 MS.gene029745:CDS
TTCGAAATGACCATACAAAC+AGG 0.512636 1.2:+47765026 None:intergenic
CGTGTGTGGCGGCATGGGAG+AGG 0.513094 1.2:+47765884 None:intergenic
TTGGAGGCTACAGATGAAGA+TGG 0.525829 1.2:-47764882 MS.gene029745:CDS
CTCTCCCATGCCGCCACACA+CGG 0.526649 1.2:-47765883 MS.gene029745:CDS
TGGAGGCTACAGATGAAGAT+GGG 0.526662 1.2:-47764881 MS.gene029745:CDS
CAGGTTCAGGTAACTCTGCA+GGG 0.527694 1.2:+47763343 None:intergenic
ATTGATGAACCGGTGGAGGA+TGG 0.527986 1.2:-47765069 MS.gene029745:CDS
GGTCACCGTGTGTGGCGGCA+TGG 0.532031 1.2:+47765878 None:intergenic
GAGTGGTAGTATTGATGAAC+CGG 0.535008 1.2:-47765079 MS.gene029745:CDS
GGCGTTGAGGTCACCGTGTG+TGG 0.535593 1.2:+47765870 None:intergenic
CCTCTTGCAGCATGATGAAG+AGG 0.537041 1.2:+47763512 None:intergenic
CCTGCAGAGTTACCTGAACC+TGG 0.542445 1.2:-47763342 MS.gene029745:CDS
CCTGCACAAGCATCGTGTAA+AGG 0.546613 1.2:+47764852 None:intergenic
AATGCTATATTAGATTTCCA+TGG 0.546731 1.2:+47763281 None:intergenic
TTGCAGCTGCTACTAGAAAG+AGG 0.551764 1.2:-47764912 MS.gene029745:intron
TTCATCTGTAGCCTCCAAGT+TGG 0.553926 1.2:+47764887 None:intergenic
CTCTTCATCATGCTGCAAGA+GGG 0.557025 1.2:-47763511 MS.gene029745:CDS
CCTCTTCATCATGCTGCAAG+AGG 0.568776 1.2:-47763512 MS.gene029745:CDS
TTACACGATGCTTGTGCAGG+AGG 0.583004 1.2:-47764849 MS.gene029745:intron
GCAGTATCCCCATCCTCCAC+CGG 0.583111 1.2:+47765060 None:intergenic
AAAGCAAAACTTATATGGAA+AGG 0.588451 1.2:-47763438 MS.gene029745:intron
TCTTCATCATGCTGCAAGAG+GGG 0.593759 1.2:-47763510 MS.gene029745:CDS
GCTTGAATCAGTTGATTCTG+AGG 0.595184 1.2:-47764087 MS.gene029745:CDS
TGATGCTGCTGCTACTGCCA+TGG 0.597475 1.2:-47763298 MS.gene029745:CDS
TGGGAGAGGTCACGAAGATG+AGG 0.605218 1.2:+47765898 None:intergenic
GTTGAGGTCACCGTGTGTGG+CGG 0.607185 1.2:+47765873 None:intergenic
ACAATGCTGAGCATATAAGA+AGG 0.607273 1.2:-47764112 MS.gene029745:CDS
GAGCGAGGCGGAGGGCGTTG+AGG 0.610700 1.2:+47765857 None:intergenic
AGGTTCAGGTAACTCTGCAG+GGG 0.613740 1.2:+47763344 None:intergenic
CTGAACCTGGCACAGATGCA+AGG 0.614501 1.2:-47763329 MS.gene029745:CDS
GGTACCGAGAGCGAGGCGGA+GGG 0.616661 1.2:+47765849 None:intergenic
TAGTATTGATGAACCGGTGG+AGG 0.618172 1.2:-47765073 MS.gene029745:CDS
GCGGTTCCAGGAAGACGCAA+TGG 0.619848 1.2:-47766000 MS.gene029745:CDS
GAGAGGTCACGAAGATGAGG+AGG 0.622353 1.2:+47765901 None:intergenic
AGAAAGAGGAGCCAACTTGG+AGG 0.623979 1.2:-47764898 MS.gene029745:CDS
CTTGAATCAGTTGATTCTGA+GGG 0.651281 1.2:-47764086 MS.gene029745:CDS
GAAGACGATGAAGAATCGAA+TGG 0.659423 1.2:-47765967 MS.gene029745:CDS
GGAGGCTACAGATGAAGATG+GGG 0.668284 1.2:-47764880 MS.gene029745:CDS
TGGTAGTATTGATGAACCGG+TGG 0.685008 1.2:-47765076 MS.gene029745:CDS
ATGATTGCAGATAACTTGAG+TGG 0.705707 1.2:-47765096 MS.gene029745:intron
AACCTGGCACAGATGCAAGG+AGG 0.718959 1.2:-47763326 MS.gene029745:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATGTTTATAGAAATTT+TGG + chr1.2:47764728-47764747 None:intergenic 10.0%
!!! AAAATGTTTATAGAAATTTT+GGG + chr1.2:47764727-47764746 None:intergenic 10.0%
!!! CGTTTTTTTTTTTTTTTTTT+TGG - chr1.2:47765645-47765664 MS.gene029745:intron 10.0%
!!! GATAAATTGTTTTATTTTTA+TGG - chr1.2:47763628-47763647 MS.gene029745:intron 10.0%
!!! GGTAAAAAAAAATTTTTTTA+CGG + chr1.2:47764818-47764837 None:intergenic 10.0%
!!! TTTTTGATTTTTTTATATGT+GGG + chr1.2:47764694-47764713 None:intergenic 10.0%
!! CTAATTGAACAAAATTATTT+GGG + chr1.2:47765073-47765092 None:intergenic 15.0%
!! TAATTGAACAAAATTATTTG+GGG + chr1.2:47765072-47765091 None:intergenic 15.0%
!! TATAAAATCAACTTATTTGT+GGG + chr1.2:47765039-47765058 None:intergenic 15.0%
!! TATATTTATAAATCCTGATA+TGG + chr1.2:47764551-47764570 None:intergenic 15.0%
!!! AAATAAGTTGATTTTATAGT+GGG - chr1.2:47765041-47765060 MS.gene029745:CDS 15.0%
!!! AAATAATTTTGTTCAATTAG+GGG - chr1.2:47765073-47765092 MS.gene029745:CDS 15.0%
!!! AAATGTTTATAGAAATTTTG+GGG + chr1.2:47764726-47764745 None:intergenic 15.0%
!!! AAATTGATTTTGATACATTT+AGG + chr1.2:47765523-47765542 None:intergenic 15.0%
!!! AATTGATTTTGATACATTTA+GGG + chr1.2:47765522-47765541 None:intergenic 15.0%
!!! AATTTTACGGTAATTTTTTT+TGG + chr1.2:47764944-47764963 None:intergenic 15.0%
!!! ATATATTTTGAAGTTACTAT+AGG + chr1.2:47763572-47763591 None:intergenic 15.0%
!!! ATTTTACGGTAATTTTTTTT+GGG + chr1.2:47764943-47764962 None:intergenic 15.0%
!!! CAAATAATTTTGTTCAATTA+GGG - chr1.2:47765072-47765091 MS.gene029745:CDS 15.0%
!!! CATTGTTTTAAAAATCAAAT+TGG + chr1.2:47764050-47764069 None:intergenic 15.0%
!!! CTTTTTGATTTTTTTATATG+TGG + chr1.2:47764695-47764714 None:intergenic 15.0%
!!! GGTAAAAAATACTTTTTTTT+GGG + chr1.2:47764781-47764800 None:intergenic 15.0%
!!! GTAAAAAATACTTTTTTTTG+GGG + chr1.2:47764780-47764799 None:intergenic 15.0%
!!! TATATTTTGAAGTTACTATA+GGG + chr1.2:47763571-47763590 None:intergenic 15.0%
!!! TTTTTTTTTTTTTTTGGGAA+GGG - chr1.2:47765651-47765670 MS.gene029745:intron 15.0%
!!! TTTTTTTTTTTTTTTTGGGA+AGG - chr1.2:47765650-47765669 MS.gene029745:intron 15.0%
!! CCTAATTGAACAAAATTATT+TGG + chr1.2:47765074-47765093 None:intergenic 20.0%
!! CTATAAAATCAACTTATTTG+TGG + chr1.2:47765040-47765059 None:intergenic 20.0%
!! TACCCAAAAAAAATTAATGT+GGG - chr1.2:47765002-47765021 MS.gene029745:intron 20.0%
!! TGAAAATTCACAAAACAAAA+AGG - chr1.2:47764652-47764671 MS.gene029745:intron 20.0%
!! TTACCCAAAAAAAATTAATG+TGG - chr1.2:47765001-47765020 MS.gene029745:intron 20.0%
!! TTGAGTGTAAAAATAATGTT+TGG - chr1.2:47765426-47765445 MS.gene029745:intron 20.0%
!!! ACATTTGCTAACATAATTTT+TGG - chr1.2:47765922-47765941 MS.gene029745:CDS 20.0%
!!! ATTGATTTTGATACATTTAG+GGG + chr1.2:47765521-47765540 None:intergenic 20.0%
!!! CAAATAAGTTGATTTTATAG+TGG - chr1.2:47765040-47765059 MS.gene029745:CDS 20.0%
!!! CCAAATAATTTTGTTCAATT+AGG - chr1.2:47765071-47765090 MS.gene029745:CDS 20.0%
!!! CGGTAAAAAATACTTTTTTT+TGG + chr1.2:47764782-47764801 None:intergenic 20.0%
!!! GGGGTAAATATTTTTTTTTA+CGG + chr1.2:47764892-47764911 None:intergenic 20.0%
!!! TGGGGTAAAAAAAAATTTTA+CGG + chr1.2:47764957-47764976 None:intergenic 20.0%
!!! TTTACGGTAAAAACTTTTTT+CGG + chr1.2:47764802-47764821 None:intergenic 20.0%
!!! TTTTACGGTAATTTTTTTTG+GGG + chr1.2:47764942-47764961 None:intergenic 20.0%
!!! TTTTTTGGGTAAAAAAATTC+CGG + chr1.2:47764993-47765012 None:intergenic 20.0%
!!! TTTTTTTTTTTTTTGGGAAG+GGG - chr1.2:47765652-47765671 MS.gene029745:intron 20.0%
! AAAAAAAGAATGAATGAGCA+TGG + chr1.2:47764311-47764330 None:intergenic 25.0%
! AAAGCAAAACTTATATGGAA+AGG - chr1.2:47765855-47765874 MS.gene029745:CDS 25.0%
! AATTATGTTAGCAAATGTTG+AGG + chr1.2:47765920-47765939 None:intergenic 25.0%
! AGAAATCATGTACATAAAGT+TGG + chr1.2:47765723-47765742 None:intergenic 25.0%
! AGTGTAAAAATAATGTTTGG+AGG - chr1.2:47765429-47765448 MS.gene029745:intron 25.0%
! CCACTAATTATAACAATGTA+GGG - chr1.2:47765598-47765617 MS.gene029745:intron 25.0%
! GTGTAAAAATAATGTTTGGA+GGG - chr1.2:47765430-47765449 MS.gene029745:intron 25.0%
! TCCACTAATTATAACAATGT+AGG - chr1.2:47765597-47765616 MS.gene029745:intron 25.0%
! TTAATTAGAATAAACCCTAG+AGG - chr1.2:47765473-47765492 MS.gene029745:intron 25.0%
!! ACCACAGAAAAAATTTTTAC+CGG - chr1.2:47764835-47764854 MS.gene029745:intron 25.0%
!! ATCAAATCAACAAGATCTTT+TGG + chr1.2:47764025-47764044 None:intergenic 25.0%
!! TGTTATAATTAGTGGATGTT+GGG + chr1.2:47765593-47765612 None:intergenic 25.0%
!! TTGTTATAATTAGTGGATGT+TGG + chr1.2:47765594-47765613 None:intergenic 25.0%
!!! AAGCCCACATTAATTTTTTT+TGG + chr1.2:47765008-47765027 None:intergenic 25.0%
!!! AATTAATGTGGGCTTTTTTT+AGG - chr1.2:47765013-47765032 MS.gene029745:intron 25.0%
!!! ACGGTACAAAACTTTTTTTT+TGG + chr1.2:47764873-47764892 None:intergenic 25.0%
!!! AGCCCACATTAATTTTTTTT+GGG + chr1.2:47765007-47765026 None:intergenic 25.0%
!!! ATTCCGGTAAATTTTTTTTC+TGG + chr1.2:47764977-47764996 None:intergenic 25.0%
!!! CGGTACAAAACTTTTTTTTT+GGG + chr1.2:47764872-47764891 None:intergenic 25.0%
!!! GGTACAAAACTTTTTTTTTG+GGG + chr1.2:47764871-47764890 None:intergenic 25.0%
!!! TGATGTTACTTTTCTTTGAA+TGG - chr1.2:47764525-47764544 MS.gene029745:intron 25.0%
!!! TTCCGGTAAATTTTTTTTCT+GGG + chr1.2:47764976-47764995 None:intergenic 25.0%
!!! TTTTTGGGGTAAAAAAATTC+CGG + chr1.2:47764857-47764876 None:intergenic 25.0%
!!! TTTTTTGGGGTAAAAAATTC+CGG + chr1.2:47764929-47764948 None:intergenic 25.0%
ACCCCAGAAAAAAAATTTAC+CGG - chr1.2:47764971-47764990 MS.gene029745:intron 30.0%
ACTTGAAAGTGAAATCAGTT+TGG - chr1.2:47763769-47763788 MS.gene029745:intron 30.0%
AGCATCATAAGAATGAAGAA+AGG + chr1.2:47764163-47764182 None:intergenic 30.0%
ATAAAGTTGGAACAGGATTA+GGG + chr1.2:47765710-47765729 None:intergenic 30.0%
ATTAGATTGTTGCTGTCTAA+TGG - chr1.2:47765820-47765839 MS.gene029745:intron 30.0%
CAAGATATGGAGAACAAATA+AGG + chr1.2:47765746-47765765 None:intergenic 30.0%
CCCTACATTGTTATAATTAG+TGG + chr1.2:47765601-47765620 None:intergenic 30.0%
TACAACAATTCTCGATTGTT+TGG - chr1.2:47764077-47764096 MS.gene029745:intron 30.0%
TAGCATACTGGGAAATTATT+CGG - chr1.2:47764134-47764153 MS.gene029745:intron 30.0%
TCCACAAAGCAAAACTTATA+TGG - chr1.2:47765850-47765869 MS.gene029745:intron 30.0%
TTGTACTAAATAGCATATCG+CGG + chr1.2:47763879-47763898 None:intergenic 30.0%
! AATTAGGGGCTTTAATTTAG+TGG - chr1.2:47765087-47765106 MS.gene029745:CDS 30.0%
! AATTTCCCAGTATGCTATTT+AGG + chr1.2:47764131-47764150 None:intergenic 30.0%
! AGTTTAAAACAGTTGAACCA+TGG - chr1.2:47763999-47764018 MS.gene029745:intron 30.0%
! ATTAGGGGCTTTAATTTAGT+GGG - chr1.2:47765088-47765107 MS.gene029745:CDS 30.0%
! TCCATATAAGTTTTGCTTTG+TGG + chr1.2:47765854-47765873 None:intergenic 30.0%
! TTGACTTTGGCTTATTGAAT+AGG + chr1.2:47765275-47765294 None:intergenic 30.0%
! TTTTAATCTGTAACATGTCG+TGG - chr1.2:47764336-47764355 MS.gene029745:intron 30.0%
!!! ATCAGTTTGGTTATTTTGAG+TGG - chr1.2:47763782-47763801 MS.gene029745:intron 30.0%
!!! ATTCCGGTAAATTTTTTCTC+TGG + chr1.2:47764913-47764932 None:intergenic 30.0%
!!! TCCGGTAAAAATTTTTTCTG+TGG + chr1.2:47764839-47764858 None:intergenic 30.0%
!!! TCCGGTAAATTTTTTTTCTG+GGG + chr1.2:47764975-47764994 None:intergenic 30.0%
!!! TTATAGAAATTTTGGGGCTT+GGG + chr1.2:47764720-47764739 None:intergenic 30.0%
!!! TTCCGGTAAATTTTTTCTCT+GGG + chr1.2:47764912-47764931 None:intergenic 30.0%
!!! TTTATAGAAATTTTGGGGCT+TGG + chr1.2:47764721-47764740 None:intergenic 30.0%
ACAATGCTGAGCATATAAGA+AGG - chr1.2:47765181-47765200 MS.gene029745:intron 35.0%
ACCCCAGAGAAAAAATTTAC+CGG - chr1.2:47764907-47764926 MS.gene029745:CDS 35.0%
AGACATCCTAAATAGCATAC+TGG - chr1.2:47764122-47764141 MS.gene029745:CDS 35.0%
ATGATTGCAGATAACTTGAG+TGG - chr1.2:47764197-47764216 MS.gene029745:intron 35.0%
ATTAGGGATTAGGATTTAGC+CGG + chr1.2:47765694-47765713 None:intergenic 35.0%
CATAAAGTTGGAACAGGATT+AGG + chr1.2:47765711-47765730 None:intergenic 35.0%
CATGTACATAAAGTTGGAAC+AGG + chr1.2:47765717-47765736 None:intergenic 35.0%
CTATAGGGTTTAACAGAAAG+AGG + chr1.2:47763556-47763575 None:intergenic 35.0%
CTCTACTGTTAAGTAGAAGT+TGG + chr1.2:47764576-47764595 None:intergenic 35.0%
GACATCCTAAATAGCATACT+GGG - chr1.2:47764123-47764142 MS.gene029745:CDS 35.0%
GCGAAAATAGTCACAAGATA+TGG + chr1.2:47765759-47765778 None:intergenic 35.0%
GCTATATATCTGTTTCGTAG+CGG - chr1.2:47763919-47763938 MS.gene029745:intron 35.0%
TTCGAAATGACCATACAAAC+AGG + chr1.2:47764270-47764289 None:intergenic 35.0%
TTTCTTTGAATGGCCATATC+AGG - chr1.2:47764535-47764554 MS.gene029745:intron 35.0%
! CTTGAATCAGTTGATTCTGA+GGG - chr1.2:47765207-47765226 MS.gene029745:intron 35.0%
! TAGGAACTCACACTTTTAGA+AGG + chr1.2:47765256-47765275 None:intergenic 35.0%
! TGTTTCGTTCAATGTTGGTT+AGG - chr1.2:47763813-47763832 MS.gene029745:intron 35.0%
!! GATGTTGTTTCGTTCAATGT+TGG - chr1.2:47763808-47763827 MS.gene029745:intron 35.0%
!! TCTAGTGGGCTTTGAAATTT+GGG - chr1.2:47765127-47765146 MS.gene029745:intron 35.0%
!! TTCTAGTGGGCTTTGAAATT+TGG - chr1.2:47765126-47765145 MS.gene029745:intron 35.0%
!! TTTAATTTAGTGGGCTTGGT+GGG - chr1.2:47765097-47765116 MS.gene029745:intron 35.0%
!!! AGTTGATTTTATAGTGGGCT+TGG - chr1.2:47765046-47765065 MS.gene029745:CDS 35.0%
!!! TATAGAAATTTTGGGGCTTG+GGG + chr1.2:47764719-47764738 None:intergenic 35.0%
!!! TCCGGTAAATTTTTTCTCTG+GGG + chr1.2:47764911-47764930 None:intergenic 35.0%
AGAGAATTCTGTTTGCCTCT+AGG + chr1.2:47765491-47765510 None:intergenic 40.0%
AGTAGAGCCGTCAATATCAT+GGG - chr1.2:47764589-47764608 MS.gene029745:intron 40.0%
ATCTGCAATCATGCAACACA+GGG + chr1.2:47764189-47764208 None:intergenic 40.0%
CTTCATCTGACCTGTTTGTA+TGG - chr1.2:47764257-47764276 MS.gene029745:intron 40.0%
GAAGAATCGAATGGTCTCTT+CGG - chr1.2:47763335-47763354 MS.gene029745:CDS 40.0%
GAAGACGATGAAGAATCGAA+TGG - chr1.2:47763326-47763345 MS.gene029745:CDS 40.0%
GAGAATTCTGTTTGCCTCTA+GGG + chr1.2:47765490-47765509 None:intergenic 40.0%
GAGACAGAAAATTTGTGCAG+TGG + chr1.2:47763516-47763535 None:intergenic 40.0%
GGGATCAAATGAGTAGATAC+CGG - chr1.2:47765672-47765691 MS.gene029745:intron 40.0%
TATCTGCAATCATGCAACAC+AGG + chr1.2:47764190-47764209 None:intergenic 40.0%
TGGATGTTGGGATTCAGTTT+GGG + chr1.2:47765581-47765600 None:intergenic 40.0%
TTGGAACAGGATTAGGGATT+AGG + chr1.2:47765704-47765723 None:intergenic 40.0%
! GCTTGAATCAGTTGATTCTG+AGG - chr1.2:47765206-47765225 MS.gene029745:intron 40.0%
! TCAACAAGATCTTTTGGCCA+TGG + chr1.2:47764019-47764038 None:intergenic 40.0%
!! ACGAGTCATTGTTTCTGTGT+GGG - chr1.2:47763665-47763684 MS.gene029745:intron 40.0%
!! CTAGTGGGCTTTGAAATTTG+GGG - chr1.2:47765128-47765147 MS.gene029745:intron 40.0%
!! CTTTAATTTAGTGGGCTTGG+TGG - chr1.2:47765096-47765115 MS.gene029745:intron 40.0%
!! GAGTGGTAGTATTGATGAAC+CGG - chr1.2:47764214-47764233 MS.gene029745:intron 40.0%
!! TACGAGTCATTGTTTCTGTG+TGG - chr1.2:47763664-47763683 MS.gene029745:intron 40.0%
!! TTAATTTAGTGGGCTTGGTG+GGG - chr1.2:47765098-47765117 MS.gene029745:intron 40.0%
!!! ATAGAAATTTTGGGGCTTGG+GGG + chr1.2:47764718-47764737 None:intergenic 40.0%
AAGTCAATCCAACGACCTTC+TGG - chr1.2:47765288-47765307 MS.gene029745:intron 45.0%
AATGGTCTCTTCGGAGAAGA+AGG - chr1.2:47763344-47763363 MS.gene029745:CDS 45.0%
ACTAGAAAGAGGAGCCAACT+TGG - chr1.2:47764392-47764411 MS.gene029745:intron 45.0%
ATGTGGGCTTGCATCAATGT+GGG + chr1.2:47764678-47764697 None:intergenic 45.0%
CAGTAGAGCCGTCAATATCA+TGG - chr1.2:47764588-47764607 MS.gene029745:intron 45.0%
CTCTTCATCATGCTGCAAGA+GGG - chr1.2:47765782-47765801 MS.gene029745:intron 45.0%
GGATGTTGGGATTCAGTTTG+GGG + chr1.2:47765580-47765599 None:intergenic 45.0%
GGTTAGGCGTTATAGAGTAC+TGG - chr1.2:47763829-47763848 MS.gene029745:intron 45.0%
GTAGAGCCGTCAATATCATG+GGG - chr1.2:47764590-47764609 MS.gene029745:intron 45.0%
GTGGATGTTGGGATTCAGTT+TGG + chr1.2:47765582-47765601 None:intergenic 45.0%
TATGTGGGCTTGCATCAATG+TGG + chr1.2:47764679-47764698 None:intergenic 45.0%
TCAAGGCCCCATGATATTGA+CGG + chr1.2:47764599-47764618 None:intergenic 45.0%
TCTTCATCATGCTGCAAGAG+GGG - chr1.2:47765783-47765802 MS.gene029745:intron 45.0%
TGGAGGCTACAGATGAAGAT+GGG - chr1.2:47764412-47764431 MS.gene029745:intron 45.0%
TTCAAACCATTGCGTCTTCC+TGG + chr1.2:47763302-47763321 None:intergenic 45.0%
TTCAAGCCCCCTTACTATGT+GGG + chr1.2:47764636-47764655 None:intergenic 45.0%
TTCATCTGTAGCCTCCAAGT+TGG + chr1.2:47764409-47764428 None:intergenic 45.0%
TTGCAGCTGCTACTAGAAAG+AGG - chr1.2:47764381-47764400 MS.gene029745:intron 45.0%
TTGGAGGCTACAGATGAAGA+TGG - chr1.2:47764411-47764430 MS.gene029745:intron 45.0%
TTTCAAGCCCCCTTACTATG+TGG + chr1.2:47764637-47764656 None:intergenic 45.0%
! ATCATGGGGCCTTGAAGTTT+TGG - chr1.2:47764604-47764623 MS.gene029745:intron 45.0%
! GAAGGTCGTTGGATTGACTT+TGG + chr1.2:47765288-47765307 None:intergenic 45.0%
!! GGAGGAGTATCAGATTCTTC+AGG + chr1.2:47763377-47763396 None:intergenic 45.0%
!! GGGCTTTAATTTAGTGGGCT+TGG - chr1.2:47765093-47765112 MS.gene029745:intron 45.0%
!! TAATTTAGTGGGCTTGGTGG+GGG - chr1.2:47765099-47765118 MS.gene029745:intron 45.0%
!! TAGTATTGATGAACCGGTGG+AGG - chr1.2:47764220-47764239 MS.gene029745:intron 45.0%
!! TGGTAGTATTGATGAACCGG+TGG - chr1.2:47764217-47764236 MS.gene029745:intron 45.0%
!! AACATTAAAAATAATAATTA+AGG + chr1.2:47763605-47763624 None:intergenic 5.0%
!!! GTTTTTTTTTTTTTTTTTTT+GGG - chr1.2:47765646-47765665 MS.gene029745:intron 5.0%
AGAAAGAGGAGCCAACTTGG+AGG - chr1.2:47764395-47764414 MS.gene029745:intron 50.0%
AGAACCAACCACACACCAGA+AGG + chr1.2:47765306-47765325 None:intergenic 50.0%
AGAGAGAGAGAGAGACAGAG+GGG + chr1.2:47763469-47763488 None:intergenic 50.0%
AGAGAGAGAGAGAGAGACAG+AGG + chr1.2:47763471-47763490 None:intergenic 50.0%
AGGTTCAGGTAACTCTGCAG+GGG + chr1.2:47765952-47765971 None:intergenic 50.0%
ATTGATGAACCGGTGGAGGA+TGG - chr1.2:47764224-47764243 MS.gene029745:intron 50.0%
CAGGTTCAGGTAACTCTGCA+GGG + chr1.2:47765953-47765972 None:intergenic 50.0%
CCTCTTCATCATGCTGCAAG+AGG - chr1.2:47765781-47765800 MS.gene029745:intron 50.0%
CCTCTTGCAGCATGATGAAG+AGG + chr1.2:47765784-47765803 None:intergenic 50.0%
CCTTTACACGATGCTTGTGC+AGG - chr1.2:47764441-47764460 MS.gene029745:intron 50.0%
GAGAGAGAGAGAGAGACAGA+GGG + chr1.2:47763470-47763489 None:intergenic 50.0%
GGAGGCTACAGATGAAGATG+GGG - chr1.2:47764413-47764432 MS.gene029745:intron 50.0%
TGGTCATTTCGAATGCGCTC+AGG - chr1.2:47764277-47764296 MS.gene029745:intron 50.0%
TTACACGATGCTTGTGCAGG+AGG - chr1.2:47764444-47764463 MS.gene029745:intron 50.0%
TTGATGAACCGGTGGAGGAT+GGG - chr1.2:47764225-47764244 MS.gene029745:intron 50.0%
! CCTGCACAAGCATCGTGTAA+AGG + chr1.2:47764444-47764463 None:intergenic 50.0%
!! TGGTGGGGGCTTTTTCTAGT+GGG - chr1.2:47765113-47765132 MS.gene029745:intron 50.0%
!! TTGGTGGGGGCTTTTTCTAG+TGG - chr1.2:47765112-47765131 MS.gene029745:intron 50.0%
AACCTGGCACAGATGCAAGG+AGG - chr1.2:47765967-47765986 MS.gene029745:CDS 55.0%
ACCACACACCAGAAGGTCGT+TGG + chr1.2:47765299-47765318 None:intergenic 55.0%
ACGACCTTCTGGTGTGTGGT+TGG - chr1.2:47765299-47765318 MS.gene029745:intron 55.0%
CCAGGTTCAGGTAACTCTGC+AGG + chr1.2:47765954-47765973 None:intergenic 55.0%
CCTGCAGAGTTACCTGAACC+TGG - chr1.2:47765951-47765970 MS.gene029745:CDS 55.0%
CTGAACCTGGCACAGATGCA+AGG - chr1.2:47765964-47765983 MS.gene029745:CDS 55.0%
CTTGCATCTGTGCCAGGTTC+AGG + chr1.2:47765966-47765985 None:intergenic 55.0%
GAGAGAGAGAGAGACAGAGG+GGG + chr1.2:47763468-47763487 None:intergenic 55.0%
GAGAGGTCACGAAGATGAGG+AGG + chr1.2:47763395-47763414 None:intergenic 55.0%
TCCAACGACCTTCTGGTGTG+TGG - chr1.2:47765295-47765314 MS.gene029745:intron 55.0%
TGATGAACCGGTGGAGGATG+GGG - chr1.2:47764226-47764245 MS.gene029745:intron 55.0%
TGATGCTGCTGCTACTGCCA+TGG - chr1.2:47765995-47766014 MS.gene029745:CDS 55.0%
TGGGAGAGGTCACGAAGATG+AGG + chr1.2:47763398-47763417 None:intergenic 55.0%
AGCCCCCTTACTATGTGGGC+TGG + chr1.2:47764632-47764651 None:intergenic 60.0%
AGCCTCCTTGCATCTGTGCC+AGG + chr1.2:47765972-47765991 None:intergenic 60.0%
CCCCAGCCCACATAGTAAGG+GGG - chr1.2:47764627-47764646 MS.gene029745:intron 60.0%
GCAGTATCCCCATCCTCCAC+CGG + chr1.2:47764236-47764255 None:intergenic 60.0%
GCCCCAGCCCACATAGTAAG+GGG - chr1.2:47764626-47764645 MS.gene029745:intron 60.0%
GCCCCCTTACTATGTGGGCT+GGG + chr1.2:47764631-47764650 None:intergenic 60.0%
GCGGTTCCAGGAAGACGCAA+TGG - chr1.2:47763293-47763312 MS.gene029745:CDS 60.0%
GGCCCCAGCCCACATAGTAA+GGG - chr1.2:47764625-47764644 MS.gene029745:intron 60.0%
GGGCTGGGGCCAAAACTTCA+AGG + chr1.2:47764616-47764635 None:intergenic 60.0%
GTTGAGGTCACCGTGTGTGG+CGG + chr1.2:47763423-47763442 None:intergenic 60.0%
TGGCCCCAGCCCACATAGTA+AGG - chr1.2:47764624-47764643 MS.gene029745:intron 60.0%
! CCCCCTTACTATGTGGGCTG+GGG + chr1.2:47764630-47764649 None:intergenic 60.0%
CTCTCCCATGCCGCCACACA+CGG - chr1.2:47763410-47763429 MS.gene029745:intron 65.0%
GTCACCGTGTGTGGCGGCAT+GGG + chr1.2:47763417-47763436 None:intergenic 65.0%
! GGCGTTGAGGTCACCGTGTG+TGG + chr1.2:47763426-47763445 None:intergenic 65.0%
AACGCCCTCCGCCTCGCTCT+CGG - chr1.2:47763440-47763459 MS.gene029745:CDS 70.0%
CGTGTGTGGCGGCATGGGAG+AGG + chr1.2:47763412-47763431 None:intergenic 70.0%
GGTCACCGTGTGTGGCGGCA+TGG + chr1.2:47763418-47763437 None:intergenic 70.0%
! GGTACCGAGAGCGAGGCGGA+GGG + chr1.2:47763447-47763466 None:intergenic 70.0%
!! AGGGGGTACCGAGAGCGAGG+CGG + chr1.2:47763451-47763470 None:intergenic 70.0%
!! CAGAGGGGGTACCGAGAGCG+AGG + chr1.2:47763454-47763473 None:intergenic 70.0%
!! GAGCGAGGCGGAGGGCGTTG+AGG + chr1.2:47763439-47763458 None:intergenic 75.0%
!! GGGTACCGAGAGCGAGGCGG+AGG + chr1.2:47763448-47763467 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 47763290 47766025 47763290 ID=MS.gene029745
chr1.2 mRNA 47763290 47766025 47763290 ID=MS.gene029745.t1;Parent=MS.gene029745
chr1.2 exon 47765854 47766025 47765854 ID=MS.gene029745.t1.exon1;Parent=MS.gene029745.t1
chr1.2 CDS 47765854 47766025 47765854 ID=cds.MS.gene029745.t1;Parent=MS.gene029745.t1
chr1.2 exon 47765017 47765108 47765017 ID=MS.gene029745.t1.exon2;Parent=MS.gene029745.t1
chr1.2 CDS 47765017 47765108 47765017 ID=cds.MS.gene029745.t1;Parent=MS.gene029745.t1
chr1.2 exon 47764850 47764928 47764850 ID=MS.gene029745.t1.exon3;Parent=MS.gene029745.t1
chr1.2 CDS 47764850 47764928 47764850 ID=cds.MS.gene029745.t1;Parent=MS.gene029745.t1
chr1.2 exon 47764079 47764152 47764079 ID=MS.gene029745.t1.exon4;Parent=MS.gene029745.t1
chr1.2 CDS 47764079 47764152 47764079 ID=cds.MS.gene029745.t1;Parent=MS.gene029745.t1
chr1.2 exon 47763439 47763534 47763439 ID=MS.gene029745.t1.exon5;Parent=MS.gene029745.t1
chr1.2 CDS 47763439 47763534 47763439 ID=cds.MS.gene029745.t1;Parent=MS.gene029745.t1
chr1.2 exon 47763290 47763367 47763290 ID=MS.gene029745.t1.exon6;Parent=MS.gene029745.t1
chr1.2 CDS 47763290 47763367 47763290 ID=cds.MS.gene029745.t1;Parent=MS.gene029745.t1
Gene Sequence

>MS.gene029745

ATGGCGGTTCCAGGAAGACGCAATGGTTTGAATGAAGAAGACGATGAAGAATCGAATGGTCTCTTCGGAGAAGAAGGTCTTGTTCCTGAAGAATCTGATACTCCTCCTCATCTTCGTGACCTCTCCCATGCCGCCACACACGGTGACCTCAACGCCCTCCGCCTCGCTCTCGGTACCCCCTCTGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCCACTGCACAAATTTTCTGTCTCTGTTAATTCAATATTTACCTCTTTCTGTTAAACCCTATAGTAACTTCAAAATATATCAATCTAAAACCTTAATTATTATTTTTAATGTTTTAGATAAATTGTTTTATTTTTATGGTATTTGATTGTGATACGAGTCATTGTTTCTGTGTGGGTTGATTACATTTTTAATTTTATGTTGATTGAGATGATTTGCAGAGAAAACTTTATAATTATATGATATTTTAAAAAATTGAACTTGAAAGTGAAATCAGTTTGGTTATTTTGAGTGGTTTGATGTTGTTTCGTTCAATGTTGGTTAGGCGTTATAGAGTACTGGAATTTTGAACAAATCAGTATTGTTCCGCGATATGCTATTTAGTACAAAATGTTGTCAAATACTATTAGCTATATATCTGTTTCGTAGCGGAATTTGAACAGAGTTTTATATTTCGCAATCTGTGATTGACACTTGCAGTTTCGTCTCAGTTTAAAACAGTTGAACCATGGCCAAAAGATCTTGTTGATTTGATGTCCAATTTGATTTTTAAAACAATGAATTTTATACAACAATTCTCGATTGTTTGGATTGTTTGATATGTGAACTAAAAGACATCCTAAATAGCATACTGGGAAATTATTCGGTGTCCTTTCTTCATTCTTATGATGCTTATCCCTGTGTTGCATGATTGCAGATAACTTGAGTGGTAGTATTGATGAACCGGTGGAGGATGGGGATACTGCTCTTCATCTGACCTGTTTGTATGGTCATTTCGAATGCGCTCAGGTTTGTTATCCATGCTCATTCATTCTTTTTTTTATGATTTTAATCTGTAACATGTCGTGGATTATTGATCTTGATCAATGAATTGCAGCTGCTACTAGAAAGAGGAGCCAACTTGGAGGCTACAGATGAAGATGGGGCAATTCCTTTACACGATGCTTGTGCAGGAGGTAGTTTAAATTATCATATTTTAATTGTTGAACTTTTTAAATTTATCTGTTAAGATTTGTGATGTTACTTTTCTTTGAATGGCCATATCAGGATTTATAAATATAGTCCAACTTCTACTTAACAGTAGAGCCGTCAATATCATGGGGCCTTGAAGTTTTGGCCCCAGCCCACATAGTAAGGGGGCTTGAAAATTCACAAAACAAAAAGGCCCACATTGATGCAAGCCCACATATAAAAAAATCAAAAAGCCCCCAAGCCCCAAAATTTCTATAAACATTTTTTTTAAACTTTTTTATTTAAAAAAAAGTTACCCCAAAAAAAAGTATTTTTTACCGAAAAAAGTTTTTACCGTAAAAAAATTTTTTTTTACCACAGAAAAAATTTTTACCGGAATTTTTTTACCCCAAAAAAAAAGTTTTGTACCGTAAAAAAAAATATTTACCCCAGAGAAAAAATTTACCGGAATTTTTTACCCCAAAAAAAATTACCGTAAAATTTTTTTTTACCCCAGAAAAAAAATTTACCGGAATTTTTTTACCCAAAAAAAATTAATGTGGGCTTTTTTTAGGCCCACAAATAAGTTGATTTTATAGTGGGCTTGGCCCCAAATAATTTTGTTCAATTAGGGGCTTTAATTTAGTGGGCTTGGTGGGGGCTTTTTCTAGTGGGCTTTGAAATTTGGGGCTCAATTGACAGCTCTACTTAACAGAGCTAACAATGCTGAGCATATAAGAAGGATGCTTGAATCAGTTGATTCTGAGGGTGATACTGTGAGTATTTATTTATCCTTCTAAAAGTGTGAGTTCCTATTCAATAAGCCAAAGTCAATCCAACGACCTTCTGGTGTGTGGTTGGTTCTATGATATTGAAAATTGATTTTGATTGAATTGACTTGTAAAATTTATTTTAGAAAATAATTTATGTCTGAATGAGTTGTAAAAATGAGTTGAACAATAAATTTGAGTGTAAAAATAATGTTTGGAGGGAAAAGCTGAAAATCTTAGCTTTAATTAGAATAAACCCTAGAGGCAAACAGAATTCTCTTTGAGTACCCCTAAATGTATCAAAATCAATTTTAAATCTTCATAAATCATAATCACTTTTGACTCTCCCCAAACTGAATCCCAACATCCACTAATTATAACAATGTAGGGTACTTAATATTAAACTTATTTGTGCGTTTTTTTTTTTTTTTTTTTGGGAAGGGGATCAAATGAGTAGATACCGGCTAAATCCTAATCCCTAATCCTGTTCCAACTTTATGTACATGATTTCTCCTTATTTGTTCTCCATATCTTGTGACTATTTTCGCAGCCTCTTCATCATGCTGCAAGAGGGGAGCATGCTGATGTAATTAGATTGTTGCTGTCTAATGGTGCATCATCCACAAAGCAAAACTTATATGGAAAGGTATAGCTTTTAAACGAATTTAAAAATATCAGTTTCTAGTCCTCAACATTTGCTAACATAATTTTTGGCAGACCCCTGCAGAGTTACCTGAACCTGGCACAGATGCAAGGAGGCTGTTTGATGCTGCTGCTACTGCCATGGAAATCTAA
Protein sequence

>MS.gene029745.t1

MAVPGRRNGLNEEDDEESNGLFGEEGLVPEESDTPPHLRDLSHAATHGDLNALRLALDNLSGSIDEPVEDGDTALHLTCLYGHFECAQLLLERGANLEATDEDGAIPLHDACAGALLNRANNAEHIRRMLESVDSEGDTPLHHAARGEHADVIRLLLSNGASSTKQNLYGKTPAELPEPGTDARRLFDAAATAMEI