Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050069.t1 | XP_003615184.1 | 97.7 | 386 | 9 | 0 | 1 | 386 | 12 | 397 | 3.30E-181 | 644.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050069.t1 | Q9QYW0 | 29.5 | 312 | 179 | 9 | 85 | 357 | 183 | 492 | 1.7e-24 | 115.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050069.t1 | G7KAI8 | 97.7 | 386 | 9 | 0 | 1 | 386 | 12 | 397 | 2.4e-181 | 644.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050069 | MS.gene055603 | 0.8047 | 1.90E-49 | -1.69E-46 |
| MS.gene050069 | MS.gene06131 | 0.81633 | 5.85E-52 | -1.69E-46 |
| MS.gene050069 | MS.gene08767 | 0.801745 | 7.79E-49 | -1.69E-46 |
| MS.gene050069 | MS.gene29719 | 0.829533 | 4.88E-55 | -1.69E-46 |
| MS.gene050069 | MS.gene51111 | 0.831749 | 1.40E-55 | -1.69E-46 |
| MS.gene050069 | MS.gene57847 | 0.820294 | 7.40E-53 | -1.69E-46 |
| MS.gene050069 | MS.gene64140 | 0.808858 | 2.52E-50 | -1.69E-46 |
| MS.gene050069 | MS.gene66341 | 0.807537 | 4.81E-50 | -1.69E-46 |
| MS.gene050069 | MS.gene98889 | 0.830812 | 2.38E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene031356 | MS.gene050069 | PPI |
| MS.gene02854 | MS.gene050069 | PPI |
| MS.gene050069 | MS.gene70303 | PPI |
| MS.gene28161 | MS.gene050069 | PPI |
| MS.gene029825 | MS.gene050069 | PPI |
| MS.gene017174 | MS.gene050069 | PPI |
| MS.gene75013 | MS.gene050069 | PPI |
| MS.gene050069 | MS.gene88378 | PPI |
| MS.gene053381 | MS.gene050069 | PPI |
| MS.gene062301 | MS.gene050069 | PPI |
| MS.gene050069 | MS.gene003175 | PPI |
| MS.gene063568 | MS.gene050069 | PPI |
| MS.gene01984 | MS.gene050069 | PPI |
| MS.gene003846 | MS.gene050069 | PPI |
| MS.gene050069 | MS.gene28303 | PPI |
| MS.gene28304 | MS.gene050069 | PPI |
| MS.gene063209 | MS.gene050069 | PPI |
| MS.gene048301 | MS.gene050069 | PPI |
| MS.gene030707 | MS.gene050069 | PPI |
| MS.gene65479 | MS.gene050069 | PPI |
| MS.gene050069 | MS.gene056617 | PPI |
| MS.gene050069 | MS.gene038409 | PPI |
| MS.gene050069 | MS.gene45448 | PPI |
| MS.gene065496 | MS.gene050069 | PPI |
| MS.gene050069 | MS.gene58159 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050069.t1 | MTR_5g064850 | 97.927 | 386 | 8 | 0 | 1 | 386 | 12 | 397 | 0.0 | 780 |
| MS.gene050069.t1 | MTR_5g064850 | 97.668 | 386 | 7 | 1 | 1 | 386 | 12 | 395 | 0.0 | 772 |
| MS.gene050069.t1 | MTR_7g007890 | 95.562 | 338 | 15 | 0 | 49 | 386 | 60 | 397 | 0.0 | 674 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050069.t1 | AT5G61330 | 60.221 | 362 | 131 | 3 | 30 | 385 | 82 | 436 | 5.14e-142 | 411 |
| MS.gene050069.t1 | AT5G61330 | 60.221 | 362 | 131 | 3 | 30 | 385 | 82 | 436 | 5.14e-142 | 411 |
| MS.gene050069.t1 | AT5G61330 | 60.221 | 362 | 131 | 3 | 30 | 385 | 93 | 447 | 5.40e-142 | 411 |
Find 72 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCACGGCTTGTCCTTTC+AGG | 0.129798 | 5.4:-50500167 | None:intergenic |
| TGAAAAGATAGTAAACTTTA+TGG | 0.146420 | 5.4:+50506302 | MS.gene050069:CDS |
| CTTGCAGTATGGCAACTTTC+AGG | 0.156523 | 5.4:+50503580 | MS.gene050069:intron |
| AGATCAGCTATTAAAATATT+TGG | 0.170387 | 5.4:+50503841 | MS.gene050069:CDS |
| ACTGGTGCTGCTGCAATTAA+AGG | 0.238779 | 5.4:+50503645 | MS.gene050069:CDS |
| TTTAGAAATTTCTAATGATT+TGG | 0.246736 | 5.4:+50502592 | MS.gene050069:CDS |
| GGAGACTTTGGATTCTATAT+TGG | 0.273642 | 5.4:+50500672 | MS.gene050069:CDS |
| CATCTGCTTGATCATTCTAC+TGG | 0.336534 | 5.4:-50503809 | None:intergenic |
| ACAACTTGAGAAAGAGTATA+TGG | 0.343510 | 5.4:+50498450 | MS.gene050069:CDS |
| CATGAAGAAATCACCATTAC+TGG | 0.347783 | 5.4:-50501232 | None:intergenic |
| AACTTCTTCCAAGGAGACTT+TGG | 0.353164 | 5.4:+50500660 | MS.gene050069:CDS |
| TCATATAAGCAGCAACTTGA+TGG | 0.354843 | 5.4:-50503780 | None:intergenic |
| GGACGGTGATCCTGAACTTC+TGG | 0.355336 | 5.4:+50504721 | MS.gene050069:CDS |
| ATGCTATTCGATGATCTGTT+TGG | 0.371540 | 5.4:+50506360 | MS.gene050069:CDS |
| TCAGAATCGTCCAGAAGTTC+AGG | 0.374459 | 5.4:-50504731 | None:intergenic |
| TAGTGAGGTGGAGGAGGAAG+AGG | 0.395864 | 5.4:+50498348 | MS.gene050069:CDS |
| CCACACCTTCTGGGCTTTCA+CGG | 0.403616 | 5.4:-50500181 | None:intergenic |
| GCCATGGGAGGAAGATTTGC+CGG | 0.414884 | 5.4:-50506333 | None:intergenic |
| GAAGAACTTGAAGCATCATA+AGG | 0.425208 | 5.4:+50500134 | MS.gene050069:CDS |
| GAATCAGATGAGACAGTTAG+AGG | 0.431696 | 5.4:+50500619 | MS.gene050069:CDS |
| TTGCAGTATGGCAACTTTCA+GGG | 0.436730 | 5.4:+50503581 | MS.gene050069:intron |
| TGAGGGAAGTGATGAGGAAG+AGG | 0.439395 | 5.4:+50498381 | MS.gene050069:CDS |
| AGCCATTCTCAAGTTCAAAT+AGG | 0.443809 | 5.4:+50500474 | MS.gene050069:CDS |
| AAACAGAAACCACACCTTCT+GGG | 0.444736 | 5.4:-50500190 | None:intergenic |
| ATGACAATCTTGATCAAGAA+TGG | 0.451953 | 5.4:+50502615 | MS.gene050069:CDS |
| TGGATGGAAAGATGATGAAA+TGG | 0.453888 | 5.4:+50498426 | MS.gene050069:CDS |
| ACAATTGATCCTTCATCATC+AGG | 0.454754 | 5.4:+50504788 | MS.gene050069:CDS |
| TAATGATAGTGAGGTGGAGG+AGG | 0.458664 | 5.4:+50498342 | MS.gene050069:CDS |
| AGATTTCTCAGATGCACAAC+AGG | 0.461402 | 5.4:+50502642 | MS.gene050069:CDS |
| AGCCTATTTGAACTTGAGAA+TGG | 0.465447 | 5.4:-50500476 | None:intergenic |
| GGAGGAAGATTTGCCGGCCG+AGG | 0.466535 | 5.4:-50506327 | None:intergenic |
| TAATAATGATAGTGAGGTGG+AGG | 0.473582 | 5.4:+50498339 | MS.gene050069:CDS |
| AAGAACTGTTACCTCGTTGA+AGG | 0.475526 | 5.4:-50498223 | None:intergenic |
| GATGAGAGACAAAAGGAAAG+TGG | 0.478006 | 5.4:+50498406 | MS.gene050069:CDS |
| TATAGAATCCAAAGTCTCCT+TGG | 0.480413 | 5.4:-50500668 | None:intergenic |
| TAATGATAGCTCTAATGAAC+CGG | 0.480771 | 5.4:+50503968 | MS.gene050069:CDS |
| AGAGGAAGATGAGAGACAAA+AGG | 0.483718 | 5.4:+50498399 | MS.gene050069:CDS |
| ATCGAATAGCATTGGTGCCA+TGG | 0.493599 | 5.4:-50506349 | None:intergenic |
| AAACTTTATGGCTCCTCGGC+CGG | 0.497362 | 5.4:+50506314 | MS.gene050069:CDS |
| AGGATGACACAAGTGACAAC+TGG | 0.501309 | 5.4:+50503627 | MS.gene050069:CDS |
| AAACTCACAGCTCTTGCTCC+TGG | 0.502870 | 5.4:-50498483 | None:intergenic |
| GGAAGAGGAAGAAGATGATG+AGG | 0.505649 | 5.4:+50498363 | MS.gene050069:CDS |
| AATTGTTTACCTGATGATGA+AGG | 0.510261 | 5.4:-50504797 | None:intergenic |
| GCCGGCAAATCTTCCTCCCA+TGG | 0.513552 | 5.4:+50506332 | MS.gene050069:CDS |
| ACCATTACTGGCTTGAGCGA+TGG | 0.525229 | 5.4:-50501220 | None:intergenic |
| AGATGATGAGGGAAGTGATG+AGG | 0.528518 | 5.4:+50498375 | MS.gene050069:CDS |
| GGGACAAGTCAATCAACAAA+TGG | 0.528981 | 5.4:+50503601 | MS.gene050069:CDS |
| TGACAATCTTGATCAAGAAT+GGG | 0.532967 | 5.4:+50502616 | MS.gene050069:CDS |
| TTACTGGCTTGAGCGATGGA+TGG | 0.545707 | 5.4:-50501216 | None:intergenic |
| AGAGACAAAAGGAAAGTGGA+TGG | 0.551265 | 5.4:+50498410 | MS.gene050069:CDS |
| ATCTGCTTGATCATTCTACT+GGG | 0.552705 | 5.4:-50503808 | None:intergenic |
| AACAGATCATCGAATAGCAT+TGG | 0.555701 | 5.4:-50506357 | None:intergenic |
| TCGAATAGCATTGGTGCCAT+GGG | 0.559495 | 5.4:-50506348 | None:intergenic |
| TTCTATATTGGAACTGCAAG+AGG | 0.562013 | 5.4:+50500684 | MS.gene050069:CDS |
| AGATTTGATAACTTCTTCCA+AGG | 0.564786 | 5.4:+50500651 | MS.gene050069:CDS |
| TAGTAAACTTTATGGCTCCT+CGG | 0.577305 | 5.4:+50506310 | MS.gene050069:CDS |
| CACGGCCAACTGTGATTACG+AGG | 0.583626 | 5.4:-50506412 | None:intergenic |
| GAAGAGGAAGAAGATGATGA+GGG | 0.587899 | 5.4:+50498364 | MS.gene050069:CDS |
| TCCATCGCTCAAGCCAGTAA+TGG | 0.594131 | 5.4:+50501219 | MS.gene050069:CDS |
| GTATATGGATCTTCATCACC+AGG | 0.598970 | 5.4:+50498465 | MS.gene050069:CDS |
| AAGGATGAAGATCTCCTGAA+AGG | 0.606031 | 5.4:+50500153 | MS.gene050069:CDS |
| AAGTTCAAATAGGCTACCCC+AGG | 0.608312 | 5.4:+50500484 | MS.gene050069:CDS |
| GGTTAATAATGATAGTGAGG+TGG | 0.610351 | 5.4:+50498336 | MS.gene050069:intron |
| AATAGCATTGGTGCCATGGG+AGG | 0.612836 | 5.4:-50506345 | None:intergenic |
| ACAAGCCGTGAAAGCCCAGA+AGG | 0.615247 | 5.4:+50500176 | MS.gene050069:CDS |
| GTAGGTTAATAATGATAGTG+AGG | 0.615459 | 5.4:+50498333 | MS.gene050069:intron |
| ATTAGAGCTATCATTAACCT+CGG | 0.615774 | 5.4:-50503961 | None:intergenic |
| CTTATGTCCCCAGGTTGCCG+AGG | 0.632820 | 5.4:+50503944 | MS.gene050069:intron |
| AGTCAATCAACAAATGGCAG+AGG | 0.633983 | 5.4:+50503607 | MS.gene050069:CDS |
| TTGTGCTCAGGAAACACATG+TGG | 0.674699 | 5.4:+50504700 | MS.gene050069:intron |
| TGATGATATGTCCTTCAACG+AGG | 0.737794 | 5.4:+50498212 | MS.gene050069:CDS |
| GCTCAGGAAACACATGTGGA+CGG | 0.754092 | 5.4:+50504704 | MS.gene050069:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 50498177 | 50506419 | 50498177 | ID=MS.gene050069 |
| chr5.4 | mRNA | 50498177 | 50506419 | 50498177 | ID=MS.gene050069.t1;Parent=MS.gene050069 |
| chr5.4 | exon | 50498177 | 50498233 | 50498177 | ID=MS.gene050069.t1.exon1;Parent=MS.gene050069.t1 |
| chr5.4 | CDS | 50498177 | 50498233 | 50498177 | ID=cds.MS.gene050069.t1;Parent=MS.gene050069.t1 |
| chr5.4 | exon | 50498337 | 50498497 | 50498337 | ID=MS.gene050069.t1.exon2;Parent=MS.gene050069.t1 |
| chr5.4 | CDS | 50498337 | 50498497 | 50498337 | ID=cds.MS.gene050069.t1;Parent=MS.gene050069.t1 |
| chr5.4 | exon | 50500125 | 50500197 | 50500125 | ID=MS.gene050069.t1.exon3;Parent=MS.gene050069.t1 |
| chr5.4 | CDS | 50500125 | 50500197 | 50500125 | ID=cds.MS.gene050069.t1;Parent=MS.gene050069.t1 |
| chr5.4 | exon | 50500431 | 50500505 | 50500431 | ID=MS.gene050069.t1.exon4;Parent=MS.gene050069.t1 |
| chr5.4 | CDS | 50500431 | 50500505 | 50500431 | ID=cds.MS.gene050069.t1;Parent=MS.gene050069.t1 |
| chr5.4 | exon | 50500595 | 50500705 | 50500595 | ID=MS.gene050069.t1.exon5;Parent=MS.gene050069.t1 |
| chr5.4 | CDS | 50500595 | 50500705 | 50500595 | ID=cds.MS.gene050069.t1;Parent=MS.gene050069.t1 |
| chr5.4 | exon | 50501198 | 50501240 | 50501198 | ID=MS.gene050069.t1.exon6;Parent=MS.gene050069.t1 |
| chr5.4 | CDS | 50501198 | 50501240 | 50501198 | ID=cds.MS.gene050069.t1;Parent=MS.gene050069.t1 |
| chr5.4 | exon | 50502570 | 50502663 | 50502570 | ID=MS.gene050069.t1.exon7;Parent=MS.gene050069.t1 |
| chr5.4 | CDS | 50502570 | 50502663 | 50502570 | ID=cds.MS.gene050069.t1;Parent=MS.gene050069.t1 |
| chr5.4 | exon | 50503587 | 50503689 | 50503587 | ID=MS.gene050069.t1.exon8;Parent=MS.gene050069.t1 |
| chr5.4 | CDS | 50503587 | 50503689 | 50503587 | ID=cds.MS.gene050069.t1;Parent=MS.gene050069.t1 |
| chr5.4 | exon | 50503772 | 50503867 | 50503772 | ID=MS.gene050069.t1.exon9;Parent=MS.gene050069.t1 |
| chr5.4 | CDS | 50503772 | 50503867 | 50503772 | ID=cds.MS.gene050069.t1;Parent=MS.gene050069.t1 |
| chr5.4 | exon | 50503957 | 50503989 | 50503957 | ID=MS.gene050069.t1.exon10;Parent=MS.gene050069.t1 |
| chr5.4 | CDS | 50503957 | 50503989 | 50503957 | ID=cds.MS.gene050069.t1;Parent=MS.gene050069.t1 |
| chr5.4 | exon | 50504710 | 50504809 | 50504710 | ID=MS.gene050069.t1.exon11;Parent=MS.gene050069.t1 |
| chr5.4 | CDS | 50504710 | 50504809 | 50504710 | ID=cds.MS.gene050069.t1;Parent=MS.gene050069.t1 |
| chr5.4 | exon | 50506108 | 50506192 | 50506108 | ID=MS.gene050069.t1.exon12;Parent=MS.gene050069.t1 |
| chr5.4 | CDS | 50506108 | 50506192 | 50506108 | ID=cds.MS.gene050069.t1;Parent=MS.gene050069.t1 |
| chr5.4 | exon | 50506290 | 50506419 | 50506290 | ID=MS.gene050069.t1.exon13;Parent=MS.gene050069.t1 |
| chr5.4 | CDS | 50506290 | 50506419 | 50506290 | ID=cds.MS.gene050069.t1;Parent=MS.gene050069.t1 |
| Gene Sequence |
| Protein sequence |
