AlfalfaGEDB | MS.gene57847

M. sativa cultivar XinJiangDaYe / MS.gene57847

KEGG: K03245
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0005488 GO:0005575 GO:0005737 GO:0005852 GO:0008135 GO:0032991 GO:0045182 GO:0090079 GO:0097159 GO:0110165 GO:1901363
PFAM: PF08597.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57847.t1	XP_013452839.1	98.5	134	2	0	2	135	97	230	1.50E-59	238.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57847.t1	Q54KI0	31.7	142	82	4	2	135	85	219	6.3e-11	68.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57847.t1	I3SKP6	98.5	134	2	0	2	135	97	230	1.1e-59	238.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049140	MS.gene57847	0.809537	1.80E-50	-1.69E-46
MS.gene049166	MS.gene57847	0.839656	1.39E-57	-1.69E-46
MS.gene049756	MS.gene57847	0.809919	1.49E-50	-1.69E-46
MS.gene050069	MS.gene57847	0.820294	7.40E-53	-1.69E-46
MS.gene050114	MS.gene57847	0.844689	6.47E-59	-1.69E-46
MS.gene050277	MS.gene57847	0.808653	2.79E-50	-1.69E-46
MS.gene050317	MS.gene57847	0.850195	1.99E-60	-1.69E-46
MS.gene050320	MS.gene57847	0.814322	1.63E-51	-1.69E-46
MS.gene050838	MS.gene57847	0.857587	1.47E-62	-1.69E-46
MS.gene051003	MS.gene57847	0.848108	7.56E-60	-1.69E-46
MS.gene051233	MS.gene57847	0.801362	9.33E-49	-1.69E-46
MS.gene051245	MS.gene57847	0.806616	7.54E-50	-1.69E-46
MS.gene051343	MS.gene57847	0.82581	3.83E-54	-1.69E-46
MS.gene051676	MS.gene57847	0.810343	1.21E-50	-1.69E-46
MS.gene051700	MS.gene57847	0.801652	8.14E-49	-1.69E-46
MS.gene051746	MS.gene57847	0.823053	1.70E-53	-1.69E-46
MS.gene051748	MS.gene57847	0.832397	9.68E-56	-1.69E-46
MS.gene051756	MS.gene57847	0.847592	1.05E-59	-1.69E-46
MS.gene051757	MS.gene57847	0.801205	1.01E-48	-1.69E-46
MS.gene05188	MS.gene57847	0.931795	2.22E-94	-1.69E-46
MS.gene051902	MS.gene57847	0.819598	1.07E-52	-1.69E-46
MS.gene051903	MS.gene57847	0.85225	5.22E-61	-1.69E-46
MS.gene051975	MS.gene57847	0.878517	2.64E-69	-1.69E-46
MS.gene052342	MS.gene57847	0.801262	9.78E-49	-1.69E-46
MS.gene052434	MS.gene57847	0.851346	9.42E-61	-1.69E-46
MS.gene052818	MS.gene57847	-0.822015	2.97E-53	-1.69E-46
MS.gene052947	MS.gene57847	0.833973	3.92E-56	-1.69E-46
MS.gene053058	MS.gene57847	0.863792	1.93E-64	-1.69E-46
MS.gene053109	MS.gene57847	0.832378	9.78E-56	-1.69E-46
MS.gene053298	MS.gene57847	0.810918	9.07E-51	-1.69E-46
MS.gene053381	MS.gene57847	0.842434	2.59E-58	-1.69E-46
MS.gene053564	MS.gene57847	0.82492	6.21E-54	-1.69E-46
MS.gene053786	MS.gene57847	0.826458	2.68E-54	-1.69E-46
MS.gene053952	MS.gene57847	0.828968	6.69E-55	-1.69E-46
MS.gene053963	MS.gene57847	0.810544	1.09E-50	-1.69E-46
MS.gene05469	MS.gene57847	0.830803	2.39E-55	-1.69E-46
MS.gene05517	MS.gene57847	0.804314	2.29E-49	-1.69E-46
MS.gene055311	MS.gene57847	0.822306	2.54E-53	-1.69E-46
MS.gene055313	MS.gene57847	0.806733	7.13E-50	-1.69E-46
MS.gene055314	MS.gene57847	0.820197	7.79E-53	-1.69E-46
MS.gene055424	MS.gene57847	0.816161	6.38E-52	-1.69E-46
MS.gene055603	MS.gene57847	0.869672	2.60E-66	-1.69E-46
MS.gene056091	MS.gene57847	0.80186	7.38E-49	-1.69E-46
MS.gene056181	MS.gene57847	0.807985	3.87E-50	-1.69E-46
MS.gene056403	MS.gene57847	0.861815	7.87E-64	-1.69E-46
MS.gene056580	MS.gene57847	0.821445	4.02E-53	-1.69E-46
MS.gene056605	MS.gene57847	0.863481	2.41E-64	-1.69E-46
MS.gene056631	MS.gene57847	0.826797	2.23E-54	-1.69E-46
MS.gene056638	MS.gene57847	0.818187	2.23E-52	-1.69E-46
MS.gene056787	MS.gene57847	0.844708	6.40E-59	-1.69E-46
MS.gene05689	MS.gene57847	0.812977	3.23E-51	-1.69E-46
MS.gene056986	MS.gene57847	0.89757	1.24E-76	-1.69E-46
MS.gene056992	MS.gene57847	0.817627	2.99E-52	-1.69E-46
MS.gene057094	MS.gene57847	0.881646	2.02E-70	-1.69E-46
MS.gene057234	MS.gene57847	0.824104	9.67E-54	-1.69E-46
MS.gene05781	MS.gene57847	0.837691	4.48E-57	-1.69E-46
MS.gene057890	MS.gene57847	-0.817867	2.64E-52	-1.69E-46
MS.gene057999	MS.gene57847	0.857775	1.30E-62	-1.69E-46
MS.gene058074	MS.gene57847	0.817328	3.49E-52	-1.69E-46
MS.gene058238	MS.gene57847	0.845318	4.38E-59	-1.69E-46
MS.gene058654	MS.gene57847	0.813578	2.38E-51	-1.69E-46
MS.gene058758	MS.gene57847	0.810022	1.42E-50	-1.69E-46
MS.gene058906	MS.gene57847	0.800958	1.13E-48	-1.69E-46
MS.gene059146	MS.gene57847	0.859593	3.71E-63	-1.69E-46
MS.gene059171	MS.gene57847	0.802326	5.92E-49	-1.69E-46
MS.gene059227	MS.gene57847	0.801467	8.88E-49	-1.69E-46
MS.gene059360	MS.gene57847	0.832636	8.44E-56	-1.69E-46
MS.gene059830	MS.gene57847	0.820963	5.19E-53	-1.69E-46
MS.gene06001	MS.gene57847	0.848091	7.65E-60	-1.69E-46
MS.gene06010	MS.gene57847	0.83822	3.27E-57	-1.69E-46
MS.gene06011	MS.gene57847	0.927433	1.18E-91	-1.69E-46
MS.gene060429	MS.gene57847	0.809264	2.06E-50	-1.69E-46
MS.gene060586	MS.gene57847	0.842133	3.11E-58	-1.69E-46
MS.gene060763	MS.gene57847	0.872584	2.84E-67	-1.69E-46
MS.gene060830	MS.gene57847	0.845561	3.76E-59	-1.69E-46
MS.gene060869	MS.gene57847	0.829265	5.67E-55	-1.69E-46
MS.gene060870	MS.gene57847	0.810543	1.09E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57847.t1	MTR_6g478160	97.778	135	3	0	1	135	96	230	2.33e-93	269

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57847.t1	AT5G37475	68.657	134	40	2	2	135	94	225	2.74e-51	162
MS.gene57847.t1	AT5G37475	68.657	134	40	2	2	135	94	225	2.74e-51	162
MS.gene57847.t1	AT5G37475	68.657	134	40	2	2	135	94	225	2.74e-51	162
MS.gene57847.t1	AT1G66070	66.418	134	43	2	2	135	95	226	6.60e-51	161
MS.gene57847.t1	AT1G66070	66.418	134	43	2	2	135	95	226	6.60e-51	161

Find 19 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAGAAAAGTTATCATTATA+TGG	0.227051	6.1:-68702990	MS.gene57847:intron
GAAATCTGAGAGTGATTTCT+TGG	0.281694	6.1:-68703130	MS.gene57847:CDS
TACAGAAGACGCAATATCTT+TGG	0.316638	6.1:+68702913	None:intergenic
ATATCGATGACTTCTCTAAA+AGG	0.352339	6.1:-68702944	MS.gene57847:CDS
AAGAAATCACTCTCAGATTT+CGG	0.374125	6.1:+68703132	None:intergenic
AGTTGAAGAAGCAGATTATA+AGG	0.395629	6.1:-68703211	MS.gene57847:CDS
AAGGCCACTAAGGAATTGTT+TGG	0.397033	6.1:-68703192	MS.gene57847:CDS
GCAGCAACAAAATCTTCATC+AGG	0.455398	6.1:+68702693	None:intergenic
TGCTGATCGATATGATGCTT+TGG	0.462854	6.1:-68702673	MS.gene57847:CDS
CAGAAAAGTTATCATTATAT+GGG	0.471042	6.1:-68702989	MS.gene57847:CDS
ACCACCAAACAATTCCTTAG+TGG	0.482673	6.1:+68703188	None:intergenic
AGCAGATTATAAGGCCACTA+AGG	0.514749	6.1:-68703202	MS.gene57847:CDS
ATTTCGGTATAAAAGTATCA+AGG	0.522172	6.1:+68703148	None:intergenic
AGAAAGGTTGCGAATGAGGG+AGG	0.540753	6.1:-68703587	None:intergenic
ACTAAGGAATTGTTTGGTGG+TGG	0.543912	6.1:-68703186	MS.gene57847:CDS
GCCACTAAGGAATTGTTTGG+TGG	0.550879	6.1:-68703189	MS.gene57847:CDS
GCAGAGAAAGAAGCCAATGC+TGG	0.560128	6.1:-68702863	MS.gene57847:CDS
GAAAGGTTGCGAATGAGGGA+GGG	0.586449	6.1:-68703586	None:intergenic
ATGCAGAGCTCATCTCACAC+AGG	0.689643	6.1:-68703104	MS.gene57847:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACATTCTACAAATAGTTAAA+AGG	+	chr6.1:68703191-68703210	None:intergenic	20.0%
!!	ATAATAAAAGCAACATAGAA+AGG	+	chr6.1:68703427-68703446	None:intergenic	20.0%
!!	CAGAAAAGTTATCATTATAT+GGG	-	chr6.1:68703238-68703257	MS.gene57847:intron	20.0%
!!	TACAAATAGTTAAAAGGAAA+GGG	+	chr6.1:68703185-68703204	None:intergenic	20.0%
!!!	TCTTTTCCTTTTACTAAATT+TGG	-	chr6.1:68702895-68702914	MS.gene57847:CDS	20.0%
!	AAAATTGAAATCAATGGGAA+AGG	+	chr6.1:68703467-68703486	None:intergenic	25.0%
!	AATAGAGAACAGATATACTT+AGG	+	chr6.1:68702827-68702846	None:intergenic	25.0%
!	CATAGGAAGTAAATTAAATC+AGG	+	chr6.1:68702989-68703008	None:intergenic	25.0%
!	CTACAAATAGTTAAAAGGAA+AGG	+	chr6.1:68703186-68703205	None:intergenic	25.0%
!	GCAGAAAAGTTATCATTATA+TGG	-	chr6.1:68703237-68703256	MS.gene57847:intron	25.0%
!	GGCAAAAAATTGAAATCAAT+GGG	+	chr6.1:68703472-68703491	None:intergenic	25.0%
!!	TTTGATCTTTTCATTTGCAA+TGG	+	chr6.1:68703344-68703363	None:intergenic	25.0%
!!!	ATTTCGGTATAAAAGTATCA+AGG	+	chr6.1:68703082-68703101	None:intergenic	25.0%
!!!	GGAAAAGATAGTTTTACATA+AGG	+	chr6.1:68702883-68702902	None:intergenic	25.0%
!!!	TAGACGTTTTAATCATAGAA+AGG	+	chr6.1:68702778-68702797	None:intergenic	25.0%
	AAGAAATCACTCTCAGATTT+CGG	+	chr6.1:68703098-68703117	None:intergenic	30.0%
	AATAGACTTTGGTCAAAGAA+AGG	+	chr6.1:68702711-68702730	None:intergenic	30.0%
	ACCAAAGTCTATTTAAGTAC+AGG	-	chr6.1:68702718-68702737	MS.gene57847:CDS	30.0%
	ACCTGTACTTAAATAGACTT+TGG	+	chr6.1:68702722-68702741	None:intergenic	30.0%
	AGTTGAAGAAGCAGATTATA+AGG	-	chr6.1:68703016-68703035	MS.gene57847:intron	30.0%
	ATATCGATGACTTCTCTAAA+AGG	-	chr6.1:68703283-68703302	MS.gene57847:intron	30.0%
	CATTCATCAAGAAGAAAGTT+AGG	+	chr6.1:68703218-68703237	None:intergenic	30.0%
	CGGCAAAAAATTGAAATCAA+TGG	+	chr6.1:68703473-68703492	None:intergenic	30.0%
	GAATGAGGACATCATATAAA+AGG	-	chr6.1:68702932-68702951	MS.gene57847:CDS	30.0%
	TGACAGCCAAATTTAGTAAA+AGG	+	chr6.1:68702904-68702923	None:intergenic	30.0%
!	AACTTGCTCAAGTCTAAATT+AGG	+	chr6.1:68702966-68702985	None:intergenic	30.0%
	AATCATAGAAAGGAAGCCAA+AGG	+	chr6.1:68702768-68702787	None:intergenic	35.0%
	AATGCTGGTAAAAAGAAGAC+AGG	-	chr6.1:68703379-68703398	MS.gene57847:intron	35.0%
	ATTGAAATCAATGGGAAAGG+AGG	+	chr6.1:68703464-68703483	None:intergenic	35.0%
	GAAATCTGAGAGTGATTTCT+TGG	-	chr6.1:68703097-68703116	MS.gene57847:CDS	35.0%
	GGACATCATATAAAAGGAAC+AGG	-	chr6.1:68702938-68702957	MS.gene57847:CDS	35.0%
	TACAGAAGACGCAATATCTT+TGG	+	chr6.1:68703317-68703336	None:intergenic	35.0%
!	TGTCTTCTTTTTACCAGCAT+TGG	+	chr6.1:68703380-68703399	None:intergenic	35.0%
!	TTCTTCAACTAGTCTGTCAT+AGG	+	chr6.1:68703006-68703025	None:intergenic	35.0%
!!!	CAATTTTTTGCCGTGTCTTT+AGG	-	chr6.1:68703480-68703499	MS.gene57847:intron	35.0%
	AAGGCCACTAAGGAATTGTT+TGG	-	chr6.1:68703035-68703054	MS.gene57847:intron	40.0%
	ACCACCAAACAATTCCTTAG+TGG	+	chr6.1:68703042-68703061	None:intergenic	40.0%
	AGCAGATTATAAGGCCACTA+AGG	-	chr6.1:68703025-68703044	MS.gene57847:intron	40.0%
	GCAGCAACAAAATCTTCATC+AGG	+	chr6.1:68703537-68703556	None:intergenic	40.0%
	GCTGTCATAGTTTCTGAATG+AGG	-	chr6.1:68702917-68702936	MS.gene57847:CDS	40.0%
	TGCTGATCGATATGATGCTT+TGG	-	chr6.1:68703554-68703573	MS.gene57847:intron	40.0%
	TTTCTTGCCACCTAAAGACA+CGG	+	chr6.1:68703493-68703512	None:intergenic	40.0%
!	ACTAAGGAATTGTTTGGTGG+TGG	-	chr6.1:68703041-68703060	MS.gene57847:intron	40.0%
!!!	TTTTTTGCCGTGTCTTTAGG+TGG	-	chr6.1:68703483-68703502	MS.gene57847:intron	40.0%
	GCCACTAAGGAATTGTTTGG+TGG	-	chr6.1:68703038-68703057	MS.gene57847:intron	45.0%
	ATGCAGAGCTCATCTCACAC+AGG	-	chr6.1:68703123-68703142	MS.gene57847:CDS	50.0%
	GCAGAGAAAGAAGCCAATGC+TGG	-	chr6.1:68703364-68703383	MS.gene57847:intron	50.0%
!	ACACAGGCTTCGTGCTTTTG+AGG	-	chr6.1:68703139-68703158	MS.gene57847:CDS	50.0%
	GCGTCGCTGCAGTAGACCTT+TGG	-	chr6.1:68702749-68702768	MS.gene57847:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	68702653	68703596	68702653	ID=MS.gene57847
chr6.1	mRNA	68702653	68703596	68702653	ID=MS.gene57847.t1;Parent=MS.gene57847
chr6.1	exon	68703587	68703596	68703587	ID=MS.gene57847.t1.exon1;Parent=MS.gene57847.t1
chr6.1	CDS	68703587	68703596	68703587	ID=cds.MS.gene57847.t1;Parent=MS.gene57847.t1
chr6.1	exon	68703089	68703231	68703089	ID=MS.gene57847.t1.exon2;Parent=MS.gene57847.t1
chr6.1	CDS	68703089	68703231	68703089	ID=cds.MS.gene57847.t1;Parent=MS.gene57847.t1
chr6.1	exon	68702849	68703008	68702849	ID=MS.gene57847.t1.exon3;Parent=MS.gene57847.t1
chr6.1	CDS	68702849	68703008	68702849	ID=cds.MS.gene57847.t1;Parent=MS.gene57847.t1
chr6.1	exon	68702653	68702747	68702653	ID=MS.gene57847.t1.exon4;Parent=MS.gene57847.t1
chr6.1	CDS	68702653	68702747	68702653	ID=cds.MS.gene57847.t1;Parent=MS.gene57847.t1

Gene Sequence

>MS.gene57847

ATGAGGGAGGGTGAGTTGTACTGATGATTGATACTTTCATTTGCAAGTTCAAGCACCTTTCTTTGACCAAAGTCTATTTAAGTACAGGTAAACTTAGCGTCGCTGCAGTAGACCTTTGGCTTCCTTTCTATGATTAAAACGTCTAAACTTTAATGATTAGAAATTTCGATTCCTAAGTATATCTGTTCTCTATTTTTCATTGCAATAAACTTTATGTTTAAGTGATACCTTATGTAAAACTATCTTTTCCTTTTACTAAATTTGGCTGTCATAGTTTCTGAATGAGGACATCATATAAAAGGAACAGGTACCTAATTTAGACTTGAGCAAGTTCCTGATTTAATTTACTTCCTATGACAGACTAGTTGAAGAAGCAGATTATAAGGCCACTAAGGAATTGTTTGGTGGTGGTAACGATGAAAAGAACCTTGATACTTTTATACCGAAATCTGAGAGTGATTTCTTGGAGTATGCAGAGCTCATCTCACACAGGCTTCGTGCTTTTGAGGTCAAATACTTCTGTTTCTTACCCTTTCCTTTTAACTATTTGTAGAATGTGCATCCTAACTTTCTTCTTGATGAATGCAGAAAAGTTATCATTATATGGGTCTACTGAAGAATGTAATGAGAATATCGATGACTTCTCTAAAAGGAGCAGATGCCAAAGATATTGCGTCTTCTGTAACTGCCATTGCAAATGAAAAGATCAAAGCAGAGAAAGAAGCCAATGCTGGTAAAAAGAAGACAGGTAAGTTCAACTTAACATTAGAACCTTTCTATGTTGCTTTTATTATTCATGTTATCTTCTCCTCCTTTCCCATTGATTTCAATTTTTTGCCGTGTCTTTAGGTGGCAAGAAAAAGCAGCTTACTGTTGACAAACCTGATGAAGATTTTGTTGCTGCTGATCGATATGATGCTTTGGACGATTATGATTTTATGTGA

Protein sequence

>MS.gene57847.t1

MREVEEADYKATKELFGGGNDEKNLDTFIPKSESDFLEYAELISHRLRAFEKSYHYMGLLKNVMRISMTSLKGADAKDIASSVTAIANEKIKAEKEANAGKKKTGGKKKQLTVDKPDEDFVAADRYDALDDYDFM