Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64140.t1 | XP_003615184.1 | 97.4 | 389 | 7 | 1 | 1 | 389 | 12 | 397 | 9.00E-179 | 636.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64140.t1 | Q9QYW0 | 29.2 | 312 | 180 | 8 | 88 | 360 | 183 | 492 | 2.9e-24 | 114.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64140.t1 | G7KAI8 | 97.4 | 389 | 7 | 1 | 1 | 389 | 12 | 397 | 6.5e-179 | 636.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene64140 | 0.823423 | 1.40E-53 | -1.69E-46 |
| MS.gene050069 | MS.gene64140 | 0.808858 | 2.52E-50 | -1.69E-46 |
| MS.gene050085 | MS.gene64140 | 0.803041 | 4.21E-49 | -1.69E-46 |
| MS.gene050114 | MS.gene64140 | 0.873079 | 1.94E-67 | -1.69E-46 |
| MS.gene050317 | MS.gene64140 | 0.820502 | 6.63E-53 | -1.69E-46 |
| MS.gene05188 | MS.gene64140 | 0.811284 | 7.56E-51 | -1.69E-46 |
| MS.gene051975 | MS.gene64140 | 0.80272 | 4.91E-49 | -1.69E-46 |
| MS.gene052434 | MS.gene64140 | 0.844979 | 5.41E-59 | -1.69E-46 |
| MS.gene053058 | MS.gene64140 | 0.843074 | 1.75E-58 | -1.69E-46 |
| MS.gene053381 | MS.gene64140 | 0.857472 | 1.59E-62 | -1.69E-46 |
| MS.gene055311 | MS.gene64140 | 0.823654 | 1.23E-53 | -1.69E-46 |
| MS.gene055314 | MS.gene64140 | 0.806553 | 7.78E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene64140 | 0.801856 | 7.39E-49 | -1.69E-46 |
| MS.gene056403 | MS.gene64140 | 0.85251 | 4.40E-61 | -1.69E-46 |
| MS.gene056605 | MS.gene64140 | 0.865774 | 4.63E-65 | -1.69E-46 |
| MS.gene056787 | MS.gene64140 | 0.801327 | 9.49E-49 | -1.69E-46 |
| MS.gene056849 | MS.gene64140 | 0.803459 | 3.45E-49 | -1.69E-46 |
| MS.gene056986 | MS.gene64140 | 0.810173 | 1.31E-50 | -1.69E-46 |
| MS.gene057094 | MS.gene64140 | 0.804363 | 2.24E-49 | -1.69E-46 |
| MS.gene058646 | MS.gene64140 | 0.801575 | 8.44E-49 | -1.69E-46 |
| MS.gene058654 | MS.gene64140 | 0.809169 | 2.16E-50 | -1.69E-46 |
| MS.gene059360 | MS.gene64140 | 0.822464 | 2.34E-53 | -1.69E-46 |
| MS.gene06010 | MS.gene64140 | 0.834369 | 3.12E-56 | -1.69E-46 |
| MS.gene06011 | MS.gene64140 | 0.839206 | 1.82E-57 | -1.69E-46 |
| MS.gene060296 | MS.gene64140 | 0.826841 | 2.17E-54 | -1.69E-46 |
| MS.gene060763 | MS.gene64140 | 0.814392 | 1.58E-51 | -1.69E-46 |
| MS.gene060869 | MS.gene64140 | 0.829806 | 4.19E-55 | -1.69E-46 |
| MS.gene060870 | MS.gene64140 | 0.82666 | 2.40E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene64140 | MS.gene063209 | PPI |
| MS.gene64140 | MS.gene02854 | PPI |
| MS.gene64140 | MS.gene58159 | PPI |
| MS.gene017174 | MS.gene64140 | PPI |
| MS.gene64140 | MS.gene048301 | PPI |
| MS.gene64140 | MS.gene030707 | PPI |
| MS.gene64140 | MS.gene053381 | PPI |
| MS.gene64140 | MS.gene029825 | PPI |
| MS.gene64140 | MS.gene70303 | PPI |
| MS.gene64140 | MS.gene003175 | PPI |
| MS.gene64140 | MS.gene01984 | PPI |
| MS.gene64140 | MS.gene065496 | PPI |
| MS.gene64140 | MS.gene88378 | PPI |
| MS.gene64140 | MS.gene28303 | PPI |
| MS.gene64140 | MS.gene062301 | PPI |
| MS.gene64140 | MS.gene003846 | PPI |
| MS.gene64140 | MS.gene28304 | PPI |
| MS.gene64140 | MS.gene28161 | PPI |
| MS.gene64140 | MS.gene65479 | PPI |
| MS.gene64140 | MS.gene75013 | PPI |
| MS.gene64140 | MS.gene063568 | PPI |
| MS.gene64140 | MS.gene038409 | PPI |
| MS.gene64140 | MS.gene45448 | PPI |
| MS.gene64140 | MS.gene056617 | PPI |
| MS.gene031356 | MS.gene64140 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64140.t1 | MTR_5g064850 | 97.429 | 389 | 7 | 1 | 1 | 389 | 12 | 397 | 0.0 | 775 |
| MS.gene64140.t1 | MTR_5g064850 | 97.172 | 389 | 6 | 2 | 1 | 389 | 12 | 395 | 0.0 | 767 |
| MS.gene64140.t1 | MTR_7g007890 | 96.154 | 338 | 13 | 0 | 52 | 389 | 60 | 397 | 0.0 | 678 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64140.t1 | AT5G61330 | 59.838 | 371 | 135 | 3 | 18 | 388 | 80 | 436 | 1.17e-143 | 415 |
| MS.gene64140.t1 | AT5G61330 | 59.838 | 371 | 135 | 3 | 18 | 388 | 80 | 436 | 1.17e-143 | 415 |
| MS.gene64140.t1 | AT5G61330 | 59.838 | 371 | 135 | 3 | 18 | 388 | 91 | 447 | 1.36e-143 | 416 |
Find 74 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCACAGCTTGTCCTTTC+AGG | 0.086562 | 5.1:-49752116 | None:intergenic |
| TGAAAAGATAGTAAACTTTA+TGG | 0.146420 | 5.1:+49757807 | MS.gene64140:CDS |
| CTTGCAGTATGGCAACTTTC+AGG | 0.156523 | 5.1:+49755090 | MS.gene64140:intron |
| AGATCAGCTATTAAAATATT+TGG | 0.170387 | 5.1:+49755351 | MS.gene64140:CDS |
| ACTGGTGCTGCTGCAATTAA+AGG | 0.238779 | 5.1:+49755155 | MS.gene64140:CDS |
| TTTAGAAATTTCTAATGATT+TGG | 0.246736 | 5.1:+49754541 | MS.gene64140:CDS |
| GGAGACTTTGGATTCTATAT+TGG | 0.273642 | 5.1:+49752617 | MS.gene64140:CDS |
| CATCTGCTTGATCATTCTAC+TGG | 0.336534 | 5.1:-49755319 | None:intergenic |
| ACAACTTGAGAAAGAGTATA+TGG | 0.343510 | 5.1:+49750337 | MS.gene64140:CDS |
| CATGAAGAAATCACCATTAC+TGG | 0.347783 | 5.1:-49753183 | None:intergenic |
| AACTTCTTCCAAGGAGACTT+TGG | 0.353164 | 5.1:+49752605 | MS.gene64140:CDS |
| TCATATAAGCAGCAACTTGA+TGG | 0.354843 | 5.1:-49755290 | None:intergenic |
| ATGCTATTCGATGATCTGTT+TGG | 0.371540 | 5.1:+49757865 | MS.gene64140:CDS |
| AGAAGATGATGGAGAGGTTG+AGG | 0.372751 | 5.1:+49750250 | MS.gene64140:CDS |
| TCAGAATCGTCCAGAAGTTC+AGG | 0.374459 | 5.1:-49756240 | None:intergenic |
| GGATGGTGATCCTGAACTTC+TGG | 0.382113 | 5.1:+49756230 | MS.gene64140:CDS |
| TAGTGAGGTGGAGGAGGAAG+AGG | 0.395864 | 5.1:+49750226 | MS.gene64140:CDS |
| AGAAGAAGATGAGGGACAAA+AGG | 0.412567 | 5.1:+49750286 | MS.gene64140:CDS |
| GCCATGGGAGGAAGATTTGC+CGG | 0.414884 | 5.1:-49757838 | None:intergenic |
| TGATGATAGTGAGGTGGAGG+AGG | 0.415199 | 5.1:+49750220 | MS.gene64140:CDS |
| AAGAATTGTTACCTTGTTGA+AGG | 0.419515 | 5.1:-49750116 | None:intergenic |
| GAAGAACTTGAAGCATCATA+AGG | 0.425208 | 5.1:+49752083 | MS.gene64140:CDS |
| GAATCAGATGAGACAGTTAG+AGG | 0.431696 | 5.1:+49752564 | MS.gene64140:CDS |
| TTGCAGTATGGCAACTTTCA+GGG | 0.436730 | 5.1:+49755091 | MS.gene64140:intron |
| AGCCATTCTCAAGTTCAAAT+AGG | 0.443809 | 5.1:+49752419 | MS.gene64140:CDS |
| AAACAGAAACCACACCTTCT+GGG | 0.444736 | 5.1:-49752139 | None:intergenic |
| ATGACAATCTTGATCAAGAA+TGG | 0.451953 | 5.1:+49754564 | MS.gene64140:CDS |
| GAAGATGATGGAGAGGTTGA+GGG | 0.453482 | 5.1:+49750251 | MS.gene64140:CDS |
| TGGATGGAAAGATGATGAAA+TGG | 0.453888 | 5.1:+49750313 | MS.gene64140:CDS |
| ACAATTGATCCTTCATCATC+AGG | 0.454754 | 5.1:+49756297 | MS.gene64140:CDS |
| AGATTTCTCAGATGCACAAC+AGG | 0.461402 | 5.1:+49754591 | MS.gene64140:CDS |
| AGCCTATTTGAACTTGAGAA+TGG | 0.465447 | 5.1:-49752421 | None:intergenic |
| GGAGGAAGATTTGCCGGCCG+AGG | 0.466535 | 5.1:-49757832 | None:intergenic |
| TATAGAATCCAAAGTCTCCT+TGG | 0.480413 | 5.1:-49752613 | None:intergenic |
| TAATGATAGCTCTAATGAAC+CGG | 0.480771 | 5.1:+49755478 | MS.gene64140:CDS |
| ATCGAATAGCATTGGTGCCA+TGG | 0.493599 | 5.1:-49757854 | None:intergenic |
| AAACTTTATGGCTCCTCGGC+CGG | 0.497362 | 5.1:+49757819 | MS.gene64140:CDS |
| GATGAGGGACAAAAGGAAAG+TGG | 0.500545 | 5.1:+49750293 | MS.gene64140:CDS |
| AGGATGACACAAGTGACAAC+TGG | 0.501309 | 5.1:+49755137 | MS.gene64140:CDS |
| AAACTCACAGCTCTTGCTCC+TGG | 0.502870 | 5.1:-49750370 | None:intergenic |
| AATTGTTTACCTGATGATGA+AGG | 0.510261 | 5.1:-49756306 | None:intergenic |
| GCCGGCAAATCTTCCTCCCA+TGG | 0.513552 | 5.1:+49757837 | MS.gene64140:CDS |
| TAATGATGATAGTGAGGTGG+AGG | 0.516445 | 5.1:+49750217 | MS.gene64140:CDS |
| GAGGAAGAGGAAGAAGATGA+TGG | 0.516558 | 5.1:+49750239 | MS.gene64140:CDS |
| AGAGGAAGAAGATGATGGAG+AGG | 0.518108 | 5.1:+49750244 | MS.gene64140:CDS |
| ACCATTACTGGCTTGAGCGA+TGG | 0.525229 | 5.1:-49753171 | None:intergenic |
| GGGACAAGTCAATCAACAAA+TGG | 0.528981 | 5.1:+49755111 | MS.gene64140:CDS |
| TGACAATCTTGATCAAGAAT+GGG | 0.532967 | 5.1:+49754565 | MS.gene64140:CDS |
| CGATGAGGAAGAAGAAGATG+AGG | 0.533720 | 5.1:+49750277 | MS.gene64140:CDS |
| AGGGACAAAAGGAAAGTGGA+TGG | 0.542308 | 5.1:+49750297 | MS.gene64140:CDS |
| TTACTGGCTTGAGCGATGGA+TGG | 0.545707 | 5.1:-49753167 | None:intergenic |
| ATCTGCTTGATCATTCTACT+GGG | 0.552705 | 5.1:-49755318 | None:intergenic |
| AACAGATCATCGAATAGCAT+TGG | 0.555701 | 5.1:-49757862 | None:intergenic |
| TCGAATAGCATTGGTGCCAT+GGG | 0.559495 | 5.1:-49757853 | None:intergenic |
| CACGGCCAACCGTGATTACG+AGG | 0.561299 | 5.1:-49757917 | None:intergenic |
| TTCTATATTGGAACTGCAAG+AGG | 0.562013 | 5.1:+49752629 | MS.gene64140:CDS |
| AGATTTGATAACTTCTTCCA+AGG | 0.564786 | 5.1:+49752596 | MS.gene64140:CDS |
| GGTTAATGATGATAGTGAGG+TGG | 0.576958 | 5.1:+49750214 | MS.gene64140:intron |
| TAGTAAACTTTATGGCTCCT+CGG | 0.577305 | 5.1:+49757815 | MS.gene64140:CDS |
| AGAGGTTGAGGGAAGCGATG+AGG | 0.589125 | 5.1:+49750262 | MS.gene64140:CDS |
| TCCATCGCTCAAGCCAGTAA+TGG | 0.594131 | 5.1:+49753170 | MS.gene64140:CDS |
| GTATATGGATCTTCATCACC+AGG | 0.598970 | 5.1:+49750352 | MS.gene64140:CDS |
| ACAAGCTGTGAAAGCCCAGA+AGG | 0.601029 | 5.1:+49752125 | MS.gene64140:CDS |
| AAGGATGAAGATCTCCTGAA+AGG | 0.606031 | 5.1:+49752102 | MS.gene64140:CDS |
| AAGTTCAAATAGGCTACCCC+AGG | 0.608312 | 5.1:+49752429 | MS.gene64140:CDS |
| GTAGGTTAATGATGATAGTG+AGG | 0.609411 | 5.1:+49750211 | MS.gene64140:intron |
| AATAGCATTGGTGCCATGGG+AGG | 0.612836 | 5.1:-49757850 | None:intergenic |
| ATTAGAGCTATCATTAACCT+CGG | 0.615774 | 5.1:-49755471 | None:intergenic |
| CTTATGTCCCCAGGTTGCCG+AGG | 0.632820 | 5.1:+49755454 | MS.gene64140:intron |
| AGTCAATCAACAAATGGCAG+AGG | 0.633983 | 5.1:+49755117 | MS.gene64140:CDS |
| TGATGATATGTCCTTCAACA+AGG | 0.635737 | 5.1:+49750105 | MS.gene64140:CDS |
| GCTCAGGAAACACATGCGGA+TGG | 0.644547 | 5.1:+49756213 | MS.gene64140:intron |
| GATGAGGAAGAAGAAGATGA+GGG | 0.660997 | 5.1:+49750278 | MS.gene64140:CDS |
| TTCTGCTCAGGAAACACATG+CGG | 0.661832 | 5.1:+49756209 | MS.gene64140:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 49750070 | 49757924 | 49750070 | ID=MS.gene64140 |
| chr5.1 | mRNA | 49750070 | 49757924 | 49750070 | ID=MS.gene64140.t1;Parent=MS.gene64140 |
| chr5.1 | exon | 49750070 | 49750126 | 49750070 | ID=MS.gene64140.t1.exon1;Parent=MS.gene64140.t1 |
| chr5.1 | CDS | 49750070 | 49750126 | 49750070 | ID=cds.MS.gene64140.t1;Parent=MS.gene64140.t1 |
| chr5.1 | exon | 49750215 | 49750384 | 49750215 | ID=MS.gene64140.t1.exon2;Parent=MS.gene64140.t1 |
| chr5.1 | CDS | 49750215 | 49750384 | 49750215 | ID=cds.MS.gene64140.t1;Parent=MS.gene64140.t1 |
| chr5.1 | exon | 49752074 | 49752146 | 49752074 | ID=MS.gene64140.t1.exon3;Parent=MS.gene64140.t1 |
| chr5.1 | CDS | 49752074 | 49752146 | 49752074 | ID=cds.MS.gene64140.t1;Parent=MS.gene64140.t1 |
| chr5.1 | exon | 49752376 | 49752450 | 49752376 | ID=MS.gene64140.t1.exon4;Parent=MS.gene64140.t1 |
| chr5.1 | CDS | 49752376 | 49752450 | 49752376 | ID=cds.MS.gene64140.t1;Parent=MS.gene64140.t1 |
| chr5.1 | exon | 49752540 | 49752650 | 49752540 | ID=MS.gene64140.t1.exon5;Parent=MS.gene64140.t1 |
| chr5.1 | CDS | 49752540 | 49752650 | 49752540 | ID=cds.MS.gene64140.t1;Parent=MS.gene64140.t1 |
| chr5.1 | exon | 49753149 | 49753191 | 49753149 | ID=MS.gene64140.t1.exon6;Parent=MS.gene64140.t1 |
| chr5.1 | CDS | 49753149 | 49753191 | 49753149 | ID=cds.MS.gene64140.t1;Parent=MS.gene64140.t1 |
| chr5.1 | exon | 49754519 | 49754612 | 49754519 | ID=MS.gene64140.t1.exon7;Parent=MS.gene64140.t1 |
| chr5.1 | CDS | 49754519 | 49754612 | 49754519 | ID=cds.MS.gene64140.t1;Parent=MS.gene64140.t1 |
| chr5.1 | exon | 49755097 | 49755199 | 49755097 | ID=MS.gene64140.t1.exon8;Parent=MS.gene64140.t1 |
| chr5.1 | CDS | 49755097 | 49755199 | 49755097 | ID=cds.MS.gene64140.t1;Parent=MS.gene64140.t1 |
| chr5.1 | exon | 49755282 | 49755377 | 49755282 | ID=MS.gene64140.t1.exon9;Parent=MS.gene64140.t1 |
| chr5.1 | CDS | 49755282 | 49755377 | 49755282 | ID=cds.MS.gene64140.t1;Parent=MS.gene64140.t1 |
| chr5.1 | exon | 49755467 | 49755499 | 49755467 | ID=MS.gene64140.t1.exon10;Parent=MS.gene64140.t1 |
| chr5.1 | CDS | 49755467 | 49755499 | 49755467 | ID=cds.MS.gene64140.t1;Parent=MS.gene64140.t1 |
| chr5.1 | exon | 49756219 | 49756318 | 49756219 | ID=MS.gene64140.t1.exon11;Parent=MS.gene64140.t1 |
| chr5.1 | CDS | 49756219 | 49756318 | 49756219 | ID=cds.MS.gene64140.t1;Parent=MS.gene64140.t1 |
| chr5.1 | exon | 49757613 | 49757697 | 49757613 | ID=MS.gene64140.t1.exon12;Parent=MS.gene64140.t1 |
| chr5.1 | CDS | 49757613 | 49757697 | 49757613 | ID=cds.MS.gene64140.t1;Parent=MS.gene64140.t1 |
| chr5.1 | exon | 49757795 | 49757924 | 49757795 | ID=MS.gene64140.t1.exon13;Parent=MS.gene64140.t1 |
| chr5.1 | CDS | 49757795 | 49757924 | 49757795 | ID=cds.MS.gene64140.t1;Parent=MS.gene64140.t1 |
| Gene Sequence |
| Protein sequence |
