Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051028.t1 | XP_003627442.1 | 98.5 | 199 | 3 | 0 | 1 | 199 | 1 | 199 | 1.30E-109 | 405.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051028.t1 | Q9LUM1 | 43.2 | 206 | 104 | 6 | 1 | 195 | 23 | 226 | 2.1e-34 | 147.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051028.t1 | G7L964 | 98.5 | 199 | 3 | 0 | 1 | 199 | 1 | 199 | 9.6e-110 | 405.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051028 | MS.gene81036 | 0.807602 | 4.67E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene42098 | MS.gene051028 | PPI |
| MS.gene051028 | MS.gene87041 | PPI |
| MS.gene051028 | MS.gene42848 | PPI |
| MS.gene87767 | MS.gene051028 | PPI |
| MS.gene051028 | MS.gene87039 | PPI |
| MS.gene051028 | MS.gene42099 | PPI |
| MS.gene051028 | MS.gene87040 | PPI |
| MS.gene051028 | MS.gene25045 | PPI |
| MS.gene051028 | MS.gene87046 | PPI |
| MS.gene87043 | MS.gene051028 | PPI |
| MS.gene051028 | MS.gene87768 | PPI |
| MS.gene051028 | MS.gene81036 | PPI |
| MS.gene051028 | MS.gene87769 | PPI |
| MS.gene051028 | MS.gene42100 | PPI |
| MS.gene051028 | MS.gene42097 | PPI |
| MS.gene051028 | MS.gene87762 | PPI |
| MS.gene051028 | MS.gene42091 | PPI |
| MS.gene051028 | MS.gene42103 | PPI |
| MS.gene051028 | MS.gene87765 | PPI |
| MS.gene051028 | MS.gene42096 | PPI |
| MS.gene051028 | MS.gene87038 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051028.t1 | MTR_8g023060 | 98.492 | 199 | 3 | 0 | 1 | 199 | 1 | 199 | 1.26e-149 | 413 |
| MS.gene051028.t1 | MTR_0415s0060 | 41.748 | 206 | 107 | 6 | 1 | 199 | 1 | 200 | 6.07e-41 | 137 |
| MS.gene051028.t1 | MTR_8g097400 | 40.376 | 213 | 105 | 7 | 1 | 199 | 1 | 205 | 2.53e-39 | 134 |
| MS.gene051028.t1 | MTR_8g097380 | 35.885 | 209 | 120 | 4 | 1 | 199 | 1 | 205 | 5.66e-37 | 127 |
| MS.gene051028.t1 | MTR_8g097390 | 37.129 | 202 | 114 | 5 | 1 | 195 | 1 | 196 | 9.43e-37 | 127 |
| MS.gene051028.t1 | MTR_8g097410 | 34.615 | 208 | 116 | 5 | 1 | 195 | 1 | 201 | 1.46e-34 | 121 |
| MS.gene051028.t1 | MTR_8g097370 | 35.326 | 184 | 109 | 5 | 1 | 181 | 1 | 177 | 8.09e-29 | 107 |
| MS.gene051028.t1 | MTR_2g103850 | 34.286 | 175 | 108 | 4 | 24 | 195 | 32 | 202 | 2.31e-26 | 101 |
| MS.gene051028.t1 | MTR_4g010180 | 33.333 | 186 | 116 | 5 | 17 | 198 | 12 | 193 | 7.54e-26 | 99.4 |
| MS.gene051028.t1 | MTR_2g103840 | 33.696 | 184 | 114 | 5 | 16 | 195 | 22 | 201 | 1.29e-25 | 99.0 |
| MS.gene051028.t1 | MTR_8g099000 | 34.300 | 207 | 111 | 8 | 3 | 191 | 28 | 227 | 4.74e-23 | 92.8 |
| MS.gene051028.t1 | MTR_0559s0010 | 39.773 | 88 | 40 | 2 | 1 | 78 | 1 | 85 | 2.42e-14 | 65.9 |
| MS.gene051028.t1 | MTR_7g072270 | 27.273 | 165 | 115 | 4 | 28 | 190 | 38 | 199 | 4.64e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051028.t1 | AT3G14260 | 43.204 | 206 | 104 | 6 | 1 | 195 | 23 | 226 | 2.28e-45 | 150 |
| MS.gene051028.t1 | AT1G53875 | 41.784 | 213 | 101 | 6 | 1 | 199 | 25 | 228 | 5.40e-45 | 149 |
| MS.gene051028.t1 | AT1G53890 | 46.316 | 190 | 86 | 6 | 17 | 199 | 37 | 217 | 8.82e-45 | 148 |
| MS.gene051028.t1 | AT1G53870 | 46.316 | 190 | 86 | 6 | 17 | 199 | 37 | 217 | 8.82e-45 | 148 |
| MS.gene051028.t1 | AT1G53870 | 46.316 | 190 | 86 | 6 | 17 | 199 | 37 | 217 | 8.82e-45 | 148 |
| MS.gene051028.t1 | AT1G53890 | 46.316 | 190 | 86 | 6 | 17 | 199 | 37 | 217 | 8.82e-45 | 148 |
| MS.gene051028.t1 | AT1G63410 | 40.404 | 198 | 106 | 6 | 1 | 196 | 1 | 188 | 2.37e-37 | 128 |
| MS.gene051028.t1 | AT1G63410 | 40.404 | 198 | 106 | 6 | 1 | 196 | 1 | 188 | 5.15e-37 | 128 |
| MS.gene051028.t1 | AT1G53890 | 44.311 | 167 | 77 | 6 | 17 | 176 | 37 | 194 | 9.77e-34 | 119 |
| MS.gene051028.t1 | AT1G53870 | 44.311 | 167 | 77 | 6 | 17 | 176 | 37 | 194 | 9.77e-34 | 119 |
| MS.gene051028.t1 | AT1G53870 | 44.311 | 167 | 77 | 6 | 17 | 176 | 37 | 194 | 9.77e-34 | 119 |
| MS.gene051028.t1 | AT1G53890 | 44.311 | 167 | 77 | 6 | 17 | 176 | 37 | 194 | 9.77e-34 | 119 |
| MS.gene051028.t1 | AT1G53900 | 38.191 | 199 | 100 | 6 | 1 | 185 | 1 | 190 | 1.65e-32 | 123 |
| MS.gene051028.t1 | AT1G53900 | 38.191 | 199 | 100 | 6 | 1 | 185 | 16 | 205 | 1.96e-32 | 122 |
| MS.gene051028.t1 | AT1G53900 | 38.191 | 199 | 100 | 6 | 1 | 185 | 25 | 214 | 2.12e-32 | 122 |
| MS.gene051028.t1 | AT1G53890 | 41.317 | 167 | 70 | 6 | 17 | 176 | 37 | 182 | 3.27e-27 | 103 |
| MS.gene051028.t1 | AT2G38640 | 32.474 | 194 | 120 | 5 | 1 | 190 | 1 | 187 | 8.35e-24 | 93.6 |
| MS.gene051028.t1 | AT1G63410 | 38.608 | 158 | 85 | 6 | 1 | 156 | 1 | 148 | 3.51e-23 | 90.9 |
| MS.gene051028.t1 | AT3G15810 | 32.000 | 175 | 110 | 4 | 24 | 195 | 33 | 201 | 7.19e-21 | 86.7 |
| MS.gene051028.t1 | AT1G80120 | 33.516 | 182 | 111 | 5 | 19 | 195 | 14 | 190 | 1.71e-19 | 82.8 |
| MS.gene051028.t1 | AT5G41590 | 35.196 | 179 | 103 | 7 | 23 | 190 | 38 | 214 | 5.52e-19 | 81.6 |
Find 37 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTGATCTTTCTCTAATTA+TGG | 0.126341 | 8.3:+68941390 | MS.gene051028:CDS |
| CTCGAAAGCATGGTCCTTTC+TGG | 0.163823 | 8.3:-68941040 | None:intergenic |
| TCAACTTGTGGAATTGATTT+AGG | 0.189194 | 8.3:+68941334 | MS.gene051028:CDS |
| AAACATATAGCATCTCTAAA+TGG | 0.207239 | 8.3:+68941069 | MS.gene051028:CDS |
| AATATAAGTTGTCTAGGTTT+TGG | 0.215960 | 8.3:+68940985 | MS.gene051028:CDS |
| ATTAGAGAAAGATCAATATT+TGG | 0.259853 | 8.3:-68941385 | None:intergenic |
| TTTGTTTACTATACTTAAAA+AGG | 0.279488 | 8.3:+68940714 | MS.gene051028:CDS |
| ATTGATCTTTCTCTAATTAT+GGG | 0.289911 | 8.3:+68941391 | MS.gene051028:CDS |
| CAAGCCAGCTTGCAAAATAT+TGG | 0.302733 | 8.3:+68941166 | MS.gene051028:CDS |
| TTGGGAAGGTTACAAGCTTC+CGG | 0.354783 | 8.3:+68941004 | MS.gene051028:CDS |
| AGCAAGAGACTTTCACCCTT+TGG | 0.364562 | 8.3:+68940560 | MS.gene051028:CDS |
| AACATATAGCATCTCTAAAT+GGG | 0.428061 | 8.3:+68941070 | MS.gene051028:CDS |
| ATGAAATCTCTTGTATTGAA+TGG | 0.431267 | 8.3:+68940583 | MS.gene051028:CDS |
| TCTCTTGTATTGAATGGAAA+GGG | 0.437349 | 8.3:+68940589 | MS.gene051028:CDS |
| TTATGAAGTTGAACTTGGAT+TGG | 0.444332 | 8.3:+68941100 | MS.gene051028:CDS |
| TGTAGAGGAAGCTAGTGCTT+GGG | 0.456051 | 8.3:-68940518 | None:intergenic |
| TACAAGAGATTTCATCCAAA+GGG | 0.460680 | 8.3:-68940575 | None:intergenic |
| TCAAGTTATGAAGTTGAACT+TGG | 0.473959 | 8.3:+68941095 | MS.gene051028:CDS |
| TTCTGGTCGTTCCTTGTCGC+CGG | 0.485881 | 8.3:-68941023 | None:intergenic |
| TCTATATGCAATTTGACCGT+TGG | 0.486158 | 8.3:-68940629 | None:intergenic |
| AAGGGTGAAAGTCTCTTGCT+TGG | 0.503462 | 8.3:-68940557 | None:intergenic |
| ATCTCTTGTATTGAATGGAA+AGG | 0.536878 | 8.3:+68940588 | MS.gene051028:CDS |
| TTGTAGAGGAAGCTAGTGCT+TGG | 0.543990 | 8.3:-68940519 | None:intergenic |
| AGTGAAATTGCAAGTTGTAG+AGG | 0.544457 | 8.3:-68940533 | None:intergenic |
| TGCAGCAATATAAGTTGTCT+AGG | 0.546244 | 8.3:+68940979 | MS.gene051028:intron |
| TAGAGATCAAGTTCATCTCA+TGG | 0.549861 | 8.3:+68940675 | MS.gene051028:CDS |
| AAAGGACCATGCTTTCGAGT+TGG | 0.581337 | 8.3:+68941044 | MS.gene051028:CDS |
| AGGAGAAAGAAGTCAACTTG+TGG | 0.584551 | 8.3:+68941322 | MS.gene051028:CDS |
| ATACAAGAGATTTCATCCAA+AGG | 0.591425 | 8.3:-68940576 | None:intergenic |
| GCGACAAGGAACGACCAGAA+AGG | 0.601912 | 8.3:+68941026 | MS.gene051028:CDS |
| GTTACAAGCTTCCGGCGACA+AGG | 0.606422 | 8.3:+68941012 | MS.gene051028:CDS |
| AAATGGAGTGATAGTTGCAG+AGG | 0.607549 | 8.3:+68941193 | MS.gene051028:CDS |
| GTAGAGGAAGCTAGTGCTTG+GGG | 0.609260 | 8.3:-68940517 | None:intergenic |
| TGTACCGTCTTTGATTCCAA+CGG | 0.633701 | 8.3:+68940613 | MS.gene051028:CDS |
| TTGACCGTTGGAATCAAAGA+CGG | 0.638241 | 8.3:-68940617 | None:intergenic |
| CTCTTGTATTGAATGGAAAG+GGG | 0.645005 | 8.3:+68940590 | MS.gene051028:CDS |
| GTTCATCTCATGGATCAAAG+AGG | 0.650133 | 8.3:+68940685 | MS.gene051028:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTCTATTTTATAATTATA+TGG | - | chr8.3:68941137-68941156 | None:intergenic | 10.0% |
| !! | TTTGTTTACTATACTTAAAA+AGG | + | chr8.3:68940714-68940733 | MS.gene051028:CDS | 15.0% |
| !!! | CTATTTTATAATTATATGGT+TGG | - | chr8.3:68941133-68941152 | None:intergenic | 15.0% |
| !! | ATTAGAGAAAGATCAATATT+TGG | - | chr8.3:68941388-68941407 | None:intergenic | 20.0% |
| !! | ATTGATCTTTCTCTAATTAT+GGG | + | chr8.3:68941391-68941410 | MS.gene051028:CDS | 20.0% |
| !! | TATTGATCTTTCTCTAATTA+TGG | + | chr8.3:68941390-68941409 | MS.gene051028:CDS | 20.0% |
| !!! | ACATTTTTTTCAGAAAAGTT+TGG | + | chr8.3:68941231-68941250 | MS.gene051028:intron | 20.0% |
| ! | AAACATATAGCATCTCTAAA+TGG | + | chr8.3:68941069-68941088 | MS.gene051028:CDS | 25.0% |
| ! | AACATATAGCATCTCTAAAT+GGG | + | chr8.3:68941070-68941089 | MS.gene051028:CDS | 25.0% |
| ! | ATGAAATCTCTTGTATTGAA+TGG | + | chr8.3:68940583-68940602 | MS.gene051028:CDS | 25.0% |
| ! | TACAAACTTTGCAAGTTTAT+AGG | - | chr8.3:68940771-68940790 | None:intergenic | 25.0% |
| ! | TGCAAAATATTGGACAAAAA+TGG | + | chr8.3:68941176-68941195 | MS.gene051028:CDS | 25.0% |
| !! | AATATAAGTTGTCTAGGTTT+TGG | + | chr8.3:68940985-68941004 | MS.gene051028:CDS | 25.0% |
| !! | ATATAAGTTGTCTAGGTTTT+GGG | + | chr8.3:68940986-68941005 | MS.gene051028:CDS | 25.0% |
| ATACAAGAGATTTCATCCAA+AGG | - | chr8.3:68940579-68940598 | None:intergenic | 30.0% | |
| ATCTCTTGTATTGAATGGAA+AGG | + | chr8.3:68940588-68940607 | MS.gene051028:CDS | 30.0% | |
| TACAAGAGATTTCATCCAAA+GGG | - | chr8.3:68940578-68940597 | None:intergenic | 30.0% | |
| TCAAGTTATGAAGTTGAACT+TGG | + | chr8.3:68941095-68941114 | MS.gene051028:CDS | 30.0% | |
| TCTCTTGTATTGAATGGAAA+GGG | + | chr8.3:68940589-68940608 | MS.gene051028:CDS | 30.0% | |
| TGATAAAATAACTGATGTCG+CGG | - | chr8.3:68940808-68940827 | None:intergenic | 30.0% | |
| TTATGAAGTTGAACTTGGAT+TGG | + | chr8.3:68941100-68941119 | MS.gene051028:CDS | 30.0% | |
| ! | TCAACTTGTGGAATTGATTT+AGG | + | chr8.3:68941334-68941353 | MS.gene051028:CDS | 30.0% |
| !! | CGACATCAGTTATTTTATCA+CGG | + | chr8.3:68940808-68940827 | MS.gene051028:intron | 30.0% |
| AAGTTTGTACAACACGATTG+TGG | + | chr8.3:68940782-68940801 | MS.gene051028:intron | 35.0% | |
| AGTGAAATTGCAAGTTGTAG+AGG | - | chr8.3:68940536-68940555 | None:intergenic | 35.0% | |
| CTCTTGTATTGAATGGAAAG+GGG | + | chr8.3:68940590-68940609 | MS.gene051028:CDS | 35.0% | |
| TAGAGATCAAGTTCATCTCA+TGG | + | chr8.3:68940675-68940694 | MS.gene051028:CDS | 35.0% | |
| TCTATATGCAATTTGACCGT+TGG | - | chr8.3:68940632-68940651 | None:intergenic | 35.0% | |
| TGCAGCAATATAAGTTGTCT+AGG | + | chr8.3:68940979-68940998 | MS.gene051028:intron | 35.0% | |
| ! | AGATGATGTTTTCACAATGG+TGG | + | chr8.3:68941357-68941376 | MS.gene051028:CDS | 35.0% |
| ! | AGGAGATGATGTTTTCACAA+TGG | + | chr8.3:68941354-68941373 | MS.gene051028:CDS | 35.0% |
| ! | TTGTCCAATATTTTGCAAGC+TGG | - | chr8.3:68941173-68941192 | None:intergenic | 35.0% |
| !!! | GTTTTTTCTATGAGCAGCTA+AGG | + | chr8.3:68941302-68941321 | MS.gene051028:intron | 35.0% |
| AAATGGAGTGATAGTTGCAG+AGG | + | chr8.3:68941193-68941212 | MS.gene051028:CDS | 40.0% | |
| AGGAGAAAGAAGTCAACTTG+TGG | + | chr8.3:68941322-68941341 | MS.gene051028:CDS | 40.0% | |
| CAAGCCAGCTTGCAAAATAT+TGG | + | chr8.3:68941166-68941185 | MS.gene051028:CDS | 40.0% | |
| GTTCATCTCATGGATCAAAG+AGG | + | chr8.3:68940685-68940704 | MS.gene051028:CDS | 40.0% | |
| TGTACCGTCTTTGATTCCAA+CGG | + | chr8.3:68940613-68940632 | MS.gene051028:CDS | 40.0% | |
| TTGACCGTTGGAATCAAAGA+CGG | - | chr8.3:68940620-68940639 | None:intergenic | 40.0% | |
| ! | GTTTTACCAACTCGAAAGCA+TGG | - | chr8.3:68941053-68941072 | None:intergenic | 40.0% |
| ! | TGATGTTTTCACAATGGTGG+TGG | + | chr8.3:68941360-68941379 | MS.gene051028:CDS | 40.0% |
| !!! | AAGTTGTCTAGGTTTTGGGA+AGG | + | chr8.3:68940990-68941009 | MS.gene051028:CDS | 40.0% |
| AAGGGTGAAAGTCTCTTGCT+TGG | - | chr8.3:68940560-68940579 | None:intergenic | 45.0% | |
| AGCAAGAGACTTTCACCCTT+TGG | + | chr8.3:68940560-68940579 | MS.gene051028:CDS | 45.0% | |
| GACTGATGTAGCTGCAATTG+TGG | - | chr8.3:68940834-68940853 | None:intergenic | 45.0% | |
| TGTAGAGGAAGCTAGTGCTT+GGG | - | chr8.3:68940521-68940540 | None:intergenic | 45.0% | |
| TTGGGAAGGTTACAAGCTTC+CGG | + | chr8.3:68941004-68941023 | MS.gene051028:CDS | 45.0% | |
| TTGTAGAGGAAGCTAGTGCT+TGG | - | chr8.3:68940522-68940541 | None:intergenic | 45.0% | |
| ! | AAAGGACCATGCTTTCGAGT+TGG | + | chr8.3:68941044-68941063 | MS.gene051028:CDS | 45.0% |
| GTAGAGGAAGCTAGTGCTTG+GGG | - | chr8.3:68940520-68940539 | None:intergenic | 50.0% | |
| ! | CTCGAAAGCATGGTCCTTTC+TGG | - | chr8.3:68941043-68941062 | None:intergenic | 50.0% |
| GCGACAAGGAACGACCAGAA+AGG | + | chr8.3:68941026-68941045 | MS.gene051028:CDS | 55.0% | |
| GTTACAAGCTTCCGGCGACA+AGG | + | chr8.3:68941012-68941031 | MS.gene051028:CDS | 55.0% | |
| TTCTGGTCGTTCCTTGTCGC+CGG | - | chr8.3:68941026-68941045 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 68940502 | 68941453 | 68940502 | ID=MS.gene051028 |
| chr8.3 | mRNA | 68940502 | 68941453 | 68940502 | ID=MS.gene051028.t1;Parent=MS.gene051028 |
| chr8.3 | exon | 68940502 | 68940735 | 68940502 | ID=MS.gene051028.t1.exon1;Parent=MS.gene051028.t1 |
| chr8.3 | CDS | 68940502 | 68940735 | 68940502 | ID=cds.MS.gene051028.t1;Parent=MS.gene051028.t1 |
| chr8.3 | exon | 68940984 | 68941214 | 68940984 | ID=MS.gene051028.t1.exon2;Parent=MS.gene051028.t1 |
| chr8.3 | CDS | 68940984 | 68941214 | 68940984 | ID=cds.MS.gene051028.t1;Parent=MS.gene051028.t1 |
| chr8.3 | exon | 68941319 | 68941453 | 68941319 | ID=MS.gene051028.t1.exon3;Parent=MS.gene051028.t1 |
| chr8.3 | CDS | 68941319 | 68941453 | 68941319 | ID=cds.MS.gene051028.t1;Parent=MS.gene051028.t1 |
| Gene Sequence |
| Protein sequence |
