Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25045.t1 | XP_003627442.1 | 98 | 199 | 4 | 0 | 1 | 199 | 1 | 199 | 3.90E-109 | 404.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25045.t1 | Q9LUM1 | 42.4 | 210 | 100 | 5 | 1 | 195 | 23 | 226 | 7.8e-34 | 145.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25045.t1 | G7L964 | 98.0 | 199 | 4 | 0 | 1 | 199 | 1 | 199 | 2.8e-109 | 404.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene87769 | MS.gene25045 | PPI |
| MS.gene81036 | MS.gene25045 | PPI |
| MS.gene42848 | MS.gene25045 | PPI |
| MS.gene42103 | MS.gene25045 | PPI |
| MS.gene87038 | MS.gene25045 | PPI |
| MS.gene42098 | MS.gene25045 | PPI |
| MS.gene87043 | MS.gene25045 | PPI |
| MS.gene87765 | MS.gene25045 | PPI |
| MS.gene051028 | MS.gene25045 | PPI |
| MS.gene87767 | MS.gene25045 | PPI |
| MS.gene87039 | MS.gene25045 | PPI |
| MS.gene87046 | MS.gene25045 | PPI |
| MS.gene42099 | MS.gene25045 | PPI |
| MS.gene42091 | MS.gene25045 | PPI |
| MS.gene42100 | MS.gene25045 | PPI |
| MS.gene87040 | MS.gene25045 | PPI |
| MS.gene42096 | MS.gene25045 | PPI |
| MS.gene87768 | MS.gene25045 | PPI |
| MS.gene87041 | MS.gene25045 | PPI |
| MS.gene42097 | MS.gene25045 | PPI |
| MS.gene87762 | MS.gene25045 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25045.t1 | MTR_8g023060 | 97.990 | 199 | 4 | 0 | 1 | 199 | 1 | 199 | 4.44e-149 | 412 |
| MS.gene25045.t1 | MTR_0415s0060 | 42.233 | 206 | 106 | 6 | 1 | 199 | 1 | 200 | 1.25e-41 | 139 |
| MS.gene25045.t1 | MTR_8g097400 | 40.845 | 213 | 104 | 7 | 1 | 199 | 1 | 205 | 5.75e-40 | 135 |
| MS.gene25045.t1 | MTR_8g097390 | 37.624 | 202 | 113 | 5 | 1 | 195 | 1 | 196 | 1.71e-37 | 129 |
| MS.gene25045.t1 | MTR_8g097380 | 35.885 | 209 | 120 | 4 | 1 | 199 | 1 | 205 | 8.07e-37 | 127 |
| MS.gene25045.t1 | MTR_8g097410 | 34.615 | 208 | 116 | 5 | 1 | 195 | 1 | 201 | 1.73e-34 | 121 |
| MS.gene25045.t1 | MTR_8g097370 | 36.413 | 184 | 107 | 5 | 1 | 181 | 1 | 177 | 1.88e-29 | 109 |
| MS.gene25045.t1 | MTR_2g103850 | 34.286 | 175 | 108 | 4 | 24 | 195 | 32 | 202 | 1.59e-26 | 101 |
| MS.gene25045.t1 | MTR_4g010180 | 33.333 | 186 | 116 | 5 | 17 | 198 | 12 | 193 | 4.72e-26 | 100 |
| MS.gene25045.t1 | MTR_2g103840 | 33.696 | 184 | 114 | 5 | 16 | 195 | 22 | 201 | 8.70e-26 | 99.8 |
| MS.gene25045.t1 | MTR_8g099000 | 34.300 | 207 | 111 | 8 | 3 | 191 | 28 | 227 | 2.98e-23 | 93.2 |
| MS.gene25045.t1 | MTR_0559s0010 | 39.773 | 88 | 40 | 2 | 1 | 78 | 1 | 85 | 2.20e-14 | 65.9 |
| MS.gene25045.t1 | MTR_7g072270 | 27.273 | 165 | 115 | 4 | 28 | 190 | 38 | 199 | 3.64e-12 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25045.t1 | AT1G53875 | 42.254 | 213 | 100 | 6 | 1 | 199 | 25 | 228 | 1.06e-45 | 150 |
| MS.gene25045.t1 | AT3G14260 | 42.381 | 210 | 100 | 5 | 1 | 195 | 23 | 226 | 9.91e-45 | 148 |
| MS.gene25045.t1 | AT1G53890 | 45.789 | 190 | 87 | 6 | 17 | 199 | 37 | 217 | 4.52e-44 | 146 |
| MS.gene25045.t1 | AT1G53870 | 45.789 | 190 | 87 | 6 | 17 | 199 | 37 | 217 | 4.52e-44 | 146 |
| MS.gene25045.t1 | AT1G53870 | 45.789 | 190 | 87 | 6 | 17 | 199 | 37 | 217 | 4.52e-44 | 146 |
| MS.gene25045.t1 | AT1G53890 | 45.789 | 190 | 87 | 6 | 17 | 199 | 37 | 217 | 4.52e-44 | 146 |
| MS.gene25045.t1 | AT1G63410 | 39.899 | 198 | 107 | 6 | 1 | 196 | 1 | 188 | 1.01e-36 | 127 |
| MS.gene25045.t1 | AT1G63410 | 39.899 | 198 | 107 | 6 | 1 | 196 | 1 | 188 | 2.40e-36 | 126 |
| MS.gene25045.t1 | AT1G53900 | 38.693 | 199 | 99 | 6 | 1 | 185 | 1 | 190 | 3.63e-33 | 124 |
| MS.gene25045.t1 | AT1G53900 | 38.693 | 199 | 99 | 6 | 1 | 185 | 16 | 205 | 3.67e-33 | 125 |
| MS.gene25045.t1 | AT1G53900 | 38.693 | 199 | 99 | 6 | 1 | 185 | 25 | 214 | 4.31e-33 | 124 |
| MS.gene25045.t1 | AT1G53890 | 43.713 | 167 | 78 | 6 | 17 | 176 | 37 | 194 | 4.31e-33 | 117 |
| MS.gene25045.t1 | AT1G53870 | 43.713 | 167 | 78 | 6 | 17 | 176 | 37 | 194 | 4.31e-33 | 117 |
| MS.gene25045.t1 | AT1G53870 | 43.713 | 167 | 78 | 6 | 17 | 176 | 37 | 194 | 4.31e-33 | 117 |
| MS.gene25045.t1 | AT1G53890 | 43.713 | 167 | 78 | 6 | 17 | 176 | 37 | 194 | 4.31e-33 | 117 |
| MS.gene25045.t1 | AT1G53890 | 40.719 | 167 | 71 | 6 | 17 | 176 | 37 | 182 | 1.37e-26 | 102 |
| MS.gene25045.t1 | AT2G38640 | 31.795 | 195 | 120 | 6 | 1 | 190 | 1 | 187 | 5.76e-24 | 94.4 |
| MS.gene25045.t1 | AT1G63410 | 37.975 | 158 | 86 | 6 | 1 | 156 | 1 | 148 | 1.55e-22 | 89.4 |
| MS.gene25045.t1 | AT3G15810 | 32.000 | 175 | 110 | 4 | 24 | 195 | 33 | 201 | 4.73e-21 | 87.0 |
| MS.gene25045.t1 | AT1G80120 | 33.516 | 182 | 111 | 5 | 19 | 195 | 14 | 190 | 1.13e-19 | 83.2 |
| MS.gene25045.t1 | AT5G41590 | 33.520 | 179 | 106 | 6 | 23 | 190 | 38 | 214 | 4.08e-19 | 82.0 |
Find 37 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGAAAGCATGGTCCTTTC+TGG | 0.121895 | 8.1:-77527489 | None:intergenic |
| TATTGATCTTTCTCTAATTA+TGG | 0.126341 | 8.1:+77527840 | MS.gene25045:CDS |
| AAACATATAGCATCTCTAAA+TGG | 0.207239 | 8.1:+77527518 | MS.gene25045:CDS |
| AATATAAGTTGTCTAGGTTT+TGG | 0.215960 | 8.1:+77527434 | MS.gene25045:CDS |
| ATTAGAGAAAGATCAATATT+TGG | 0.259853 | 8.1:-77527835 | None:intergenic |
| TTTGTTTACTATACTTAAAA+AGG | 0.279488 | 8.1:+77527164 | MS.gene25045:CDS |
| ATTGATCTTTCTCTAATTAT+GGG | 0.289911 | 8.1:+77527841 | MS.gene25045:CDS |
| CAAGCCAGCTTGCAAAATAT+TGG | 0.302733 | 8.1:+77527615 | MS.gene25045:CDS |
| TTGGGAAGGTTACAAGCTTC+CGG | 0.354783 | 8.1:+77527453 | MS.gene25045:CDS |
| AGCAAGAGACTTTCACCCTT+TGG | 0.364562 | 8.1:+77527010 | MS.gene25045:CDS |
| AACATATAGCATCTCTAAAT+GGG | 0.428061 | 8.1:+77527519 | MS.gene25045:CDS |
| ATGAAATCTCTTGTATTGAA+TGG | 0.431267 | 8.1:+77527033 | MS.gene25045:CDS |
| TCTCTTGTATTGAATGGAAA+GGG | 0.437349 | 8.1:+77527039 | MS.gene25045:CDS |
| TCAACTTGTGGAATTGATCT+AGG | 0.443197 | 8.1:+77527784 | MS.gene25045:CDS |
| TTATGAAGTTGAACTTGGAT+TGG | 0.444332 | 8.1:+77527549 | MS.gene25045:CDS |
| TGTAGAGGAAGCTAGTGCTT+GGG | 0.456051 | 8.1:-77526968 | None:intergenic |
| TACAAGAGATTTCATCCAAA+GGG | 0.460680 | 8.1:-77527025 | None:intergenic |
| TCTATATGCAATTTGACCAT+TGG | 0.471133 | 8.1:-77527079 | None:intergenic |
| TCAAGTTATGAAGTTGAACT+TGG | 0.473959 | 8.1:+77527544 | MS.gene25045:CDS |
| TTCTGGTCGTTCCTTGTCGC+CGG | 0.485881 | 8.1:-77527472 | None:intergenic |
| AAGGGTGAAAGTCTCTTGCT+TGG | 0.503462 | 8.1:-77527007 | None:intergenic |
| ATCTCTTGTATTGAATGGAA+AGG | 0.536878 | 8.1:+77527038 | MS.gene25045:CDS |
| TTGTAGAGGAAGCTAGTGCT+TGG | 0.543990 | 8.1:-77526969 | None:intergenic |
| AGTGAAATTGCAAGTTGTAG+AGG | 0.544457 | 8.1:-77526983 | None:intergenic |
| TGCAGCAATATAAGTTGTCT+AGG | 0.546244 | 8.1:+77527428 | MS.gene25045:intron |
| TAGAGATCAAGTTCATCTCA+TGG | 0.549861 | 8.1:+77527125 | MS.gene25045:CDS |
| AAAGGACCATGCTTTCAAGT+TGG | 0.568351 | 8.1:+77527493 | MS.gene25045:CDS |
| TGTACCGTCTTTGATTCCAA+TGG | 0.584212 | 8.1:+77527063 | MS.gene25045:CDS |
| AGGAGAAAGAAGTCAACTTG+TGG | 0.584551 | 8.1:+77527772 | MS.gene25045:CDS |
| ATACAAGAGATTTCATCCAA+AGG | 0.591425 | 8.1:-77527026 | None:intergenic |
| TTGACCATTGGAATCAAAGA+CGG | 0.601051 | 8.1:-77527067 | None:intergenic |
| GCGACAAGGAACGACCAGAA+AGG | 0.601912 | 8.1:+77527475 | MS.gene25045:CDS |
| GTTACAAGCTTCCGGCGACA+AGG | 0.606422 | 8.1:+77527461 | MS.gene25045:CDS |
| AAATGGAGTGATAGTTGCAG+AGG | 0.607549 | 8.1:+77527642 | MS.gene25045:CDS |
| GTAGAGGAAGCTAGTGCTTG+GGG | 0.609260 | 8.1:-77526967 | None:intergenic |
| CTCTTGTATTGAATGGAAAG+GGG | 0.645005 | 8.1:+77527040 | MS.gene25045:CDS |
| GTTCATCTCATGGATCAAAG+AGG | 0.650133 | 8.1:+77527135 | MS.gene25045:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTCTATTTTATAATTATA+TGG | - | chr8.1:77527586-77527605 | None:intergenic | 10.0% |
| !! | TTTGTTTACTATACTTAAAA+AGG | + | chr8.1:77527164-77527183 | MS.gene25045:CDS | 15.0% |
| !!! | CTATTTTATAATTATATGGT+TGG | - | chr8.1:77527582-77527601 | None:intergenic | 15.0% |
| !! | ATTAGAGAAAGATCAATATT+TGG | - | chr8.1:77527838-77527857 | None:intergenic | 20.0% |
| !! | ATTGATCTTTCTCTAATTAT+GGG | + | chr8.1:77527841-77527860 | MS.gene25045:CDS | 20.0% |
| !! | TATTGATCTTTCTCTAATTA+TGG | + | chr8.1:77527840-77527859 | MS.gene25045:CDS | 20.0% |
| !!! | ACATTTTTTTCAGAAAAGTT+TGG | + | chr8.1:77527680-77527699 | MS.gene25045:intron | 20.0% |
| ! | AAACATATAGCATCTCTAAA+TGG | + | chr8.1:77527518-77527537 | MS.gene25045:CDS | 25.0% |
| ! | AACATATAGCATCTCTAAAT+GGG | + | chr8.1:77527519-77527538 | MS.gene25045:CDS | 25.0% |
| ! | ATGAAATCTCTTGTATTGAA+TGG | + | chr8.1:77527033-77527052 | MS.gene25045:CDS | 25.0% |
| ! | TACAAACTTTGCAAGTTTAT+AGG | - | chr8.1:77527221-77527240 | None:intergenic | 25.0% |
| ! | TGCAAAATATTGGACAAAAA+TGG | + | chr8.1:77527625-77527644 | MS.gene25045:CDS | 25.0% |
| !! | AATATAAGTTGTCTAGGTTT+TGG | + | chr8.1:77527434-77527453 | MS.gene25045:CDS | 25.0% |
| !! | ATATAAGTTGTCTAGGTTTT+GGG | + | chr8.1:77527435-77527454 | MS.gene25045:CDS | 25.0% |
| ATACAAGAGATTTCATCCAA+AGG | - | chr8.1:77527029-77527048 | None:intergenic | 30.0% | |
| ATCTCTTGTATTGAATGGAA+AGG | + | chr8.1:77527038-77527057 | MS.gene25045:CDS | 30.0% | |
| TACAAGAGATTTCATCCAAA+GGG | - | chr8.1:77527028-77527047 | None:intergenic | 30.0% | |
| TCAAGTTATGAAGTTGAACT+TGG | + | chr8.1:77527544-77527563 | MS.gene25045:CDS | 30.0% | |
| TCTATATGCAATTTGACCAT+TGG | - | chr8.1:77527082-77527101 | None:intergenic | 30.0% | |
| TCTCTTGTATTGAATGGAAA+GGG | + | chr8.1:77527039-77527058 | MS.gene25045:CDS | 30.0% | |
| TGATAAAATAACTGATGTCG+CGG | - | chr8.1:77527258-77527277 | None:intergenic | 30.0% | |
| TTATGAAGTTGAACTTGGAT+TGG | + | chr8.1:77527549-77527568 | MS.gene25045:CDS | 30.0% | |
| !! | CGACATCAGTTATTTTATCA+CGG | + | chr8.1:77527258-77527277 | MS.gene25045:intron | 30.0% |
| AAGTTTGTACAACACGATTG+TGG | + | chr8.1:77527232-77527251 | MS.gene25045:intron | 35.0% | |
| AGTGAAATTGCAAGTTGTAG+AGG | - | chr8.1:77526986-77527005 | None:intergenic | 35.0% | |
| CTCTTGTATTGAATGGAAAG+GGG | + | chr8.1:77527040-77527059 | MS.gene25045:CDS | 35.0% | |
| TAGAGATCAAGTTCATCTCA+TGG | + | chr8.1:77527125-77527144 | MS.gene25045:CDS | 35.0% | |
| TCAACTTGTGGAATTGATCT+AGG | + | chr8.1:77527784-77527803 | MS.gene25045:CDS | 35.0% | |
| TGCAGCAATATAAGTTGTCT+AGG | + | chr8.1:77527428-77527447 | MS.gene25045:intron | 35.0% | |
| TTGACCATTGGAATCAAAGA+CGG | - | chr8.1:77527070-77527089 | None:intergenic | 35.0% | |
| ! | AGATGATGTTTTCACAATGG+TGG | + | chr8.1:77527807-77527826 | MS.gene25045:CDS | 35.0% |
| ! | AGGAGATGATGTTTTCACAA+TGG | + | chr8.1:77527804-77527823 | MS.gene25045:CDS | 35.0% |
| ! | GTTTTACCAACTTGAAAGCA+TGG | - | chr8.1:77527502-77527521 | None:intergenic | 35.0% |
| ! | TTGTCCAATATTTTGCAAGC+TGG | - | chr8.1:77527622-77527641 | None:intergenic | 35.0% |
| !!! | GTTTTTTCTATGAGCAGCTA+AGG | + | chr8.1:77527752-77527771 | MS.gene25045:intron | 35.0% |
| AAATGGAGTGATAGTTGCAG+AGG | + | chr8.1:77527642-77527661 | MS.gene25045:CDS | 40.0% | |
| AGGAGAAAGAAGTCAACTTG+TGG | + | chr8.1:77527772-77527791 | MS.gene25045:CDS | 40.0% | |
| CAAGCCAGCTTGCAAAATAT+TGG | + | chr8.1:77527615-77527634 | MS.gene25045:CDS | 40.0% | |
| GTTCATCTCATGGATCAAAG+AGG | + | chr8.1:77527135-77527154 | MS.gene25045:CDS | 40.0% | |
| TGTACCGTCTTTGATTCCAA+TGG | + | chr8.1:77527063-77527082 | MS.gene25045:CDS | 40.0% | |
| ! | AAAGGACCATGCTTTCAAGT+TGG | + | chr8.1:77527493-77527512 | MS.gene25045:CDS | 40.0% |
| ! | TGATGTTTTCACAATGGTGG+TGG | + | chr8.1:77527810-77527829 | MS.gene25045:CDS | 40.0% |
| !!! | AAGTTGTCTAGGTTTTGGGA+AGG | + | chr8.1:77527439-77527458 | MS.gene25045:CDS | 40.0% |
| AAGGGTGAAAGTCTCTTGCT+TGG | - | chr8.1:77527010-77527029 | None:intergenic | 45.0% | |
| AGCAAGAGACTTTCACCCTT+TGG | + | chr8.1:77527010-77527029 | MS.gene25045:CDS | 45.0% | |
| GACTGATGTAGCTGCAATTG+TGG | - | chr8.1:77527284-77527303 | None:intergenic | 45.0% | |
| TGTAGAGGAAGCTAGTGCTT+GGG | - | chr8.1:77526971-77526990 | None:intergenic | 45.0% | |
| TTGGGAAGGTTACAAGCTTC+CGG | + | chr8.1:77527453-77527472 | MS.gene25045:CDS | 45.0% | |
| TTGTAGAGGAAGCTAGTGCT+TGG | - | chr8.1:77526972-77526991 | None:intergenic | 45.0% | |
| ! | CTTGAAAGCATGGTCCTTTC+TGG | - | chr8.1:77527492-77527511 | None:intergenic | 45.0% |
| !!! | TTAAAATTTTGAATTTTTTT+TGG | + | chr8.1:77527355-77527374 | MS.gene25045:intron | 5.0% |
| GTAGAGGAAGCTAGTGCTTG+GGG | - | chr8.1:77526970-77526989 | None:intergenic | 50.0% | |
| GCGACAAGGAACGACCAGAA+AGG | + | chr8.1:77527475-77527494 | MS.gene25045:CDS | 55.0% | |
| GTTACAAGCTTCCGGCGACA+AGG | + | chr8.1:77527461-77527480 | MS.gene25045:CDS | 55.0% | |
| TTCTGGTCGTTCCTTGTCGC+CGG | - | chr8.1:77527475-77527494 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 77526952 | 77527903 | 77526952 | ID=MS.gene25045 |
| chr8.1 | mRNA | 77526952 | 77527903 | 77526952 | ID=MS.gene25045.t1;Parent=MS.gene25045 |
| chr8.1 | exon | 77526952 | 77527185 | 77526952 | ID=MS.gene25045.t1.exon1;Parent=MS.gene25045.t1 |
| chr8.1 | CDS | 77526952 | 77527185 | 77526952 | ID=cds.MS.gene25045.t1;Parent=MS.gene25045.t1 |
| chr8.1 | exon | 77527433 | 77527663 | 77527433 | ID=MS.gene25045.t1.exon2;Parent=MS.gene25045.t1 |
| chr8.1 | CDS | 77527433 | 77527663 | 77527433 | ID=cds.MS.gene25045.t1;Parent=MS.gene25045.t1 |
| chr8.1 | exon | 77527769 | 77527903 | 77527769 | ID=MS.gene25045.t1.exon3;Parent=MS.gene25045.t1 |
| chr8.1 | CDS | 77527769 | 77527903 | 77527769 | ID=cds.MS.gene25045.t1;Parent=MS.gene25045.t1 |
| Gene Sequence |
| Protein sequence |
