AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45370


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 58 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTATTATCAATATCTTTA+AGG 0.122806 8.3:+79897590 None:intergenic
GTTGTTCCCACAGTCAATTC+TGG 0.240799 8.3:+79896771 None:intergenic
AAGTTCTTTATGAAGATAAA+TGG 0.252533 8.3:-79896689 MS.gene45370:CDS
CCTCATATTTGTCTGGTTGA+TGG 0.257369 8.3:+79897650 None:intergenic
AGAGATGTAATACTAGAATA+TGG 0.271881 8.3:-79896933 MS.gene45370:CDS
TCCAAACCATCTAAAAGATA+TGG 0.330391 8.3:-79896847 MS.gene45370:CDS
CATACTGCGGGAAAATCTTC+TGG 0.333743 8.3:+79897286 None:intergenic
AAAATTGCTATTGTAAATAA+AGG 0.339197 8.3:+79897381 None:intergenic
TGCGGGAAAATCTTCTGGTT+TGG 0.341368 8.3:+79897291 None:intergenic
GCGGGAAAATCTTCTGGTTT+GGG 0.360800 8.3:+79897292 None:intergenic
TTGGCTTCCTCATATTTGTC+TGG 0.377614 8.3:+79897643 None:intergenic
CCCACAGTCAATTCTGGTTC+AGG 0.384551 8.3:+79896777 None:intergenic
AAAGATATGGATTGTGCTAA+AGG 0.404578 8.3:-79896834 MS.gene45370:CDS
ATTGATATAACACCTGTTCC+TGG 0.407869 8.3:-79896666 MS.gene45370:CDS
TCTTCTGGTTTGGGAGGATC+AGG 0.413561 8.3:+79897301 None:intergenic
GGGAAACAGGTGATGAATCT+TGG 0.416565 8.3:-79896912 MS.gene45370:CDS
ATAGCTCTCCAGCACTCAAT+TGG 0.417213 8.3:-79897351 MS.gene45370:CDS
ACAATTCCTTGATGAATACT+TGG 0.419418 8.3:+79897565 None:intergenic
AATACTAGAATATGGGAAAC+AGG 0.420325 8.3:-79896925 MS.gene45370:CDS
ATTTATCTTCATAAAGAACT+TGG 0.428540 8.3:+79896691 None:intergenic
TCAAGGAAGCATGTGAAACT+TGG 0.431792 8.3:-79897534 MS.gene45370:CDS
GAATTGATGTCAGAAGCATT+AGG 0.450460 8.3:-79896876 MS.gene45370:CDS
GTTCTTGAGGAGGTGAAAGA+TGG 0.464397 8.3:-79897472 MS.gene45370:CDS
ACTATGAGAGCTCCAGGAAC+AGG 0.475780 8.3:+79896654 None:intergenic
GAACTTGGAGACCACCAATA+TGG 0.476488 8.3:+79896706 None:intergenic
GTGCTTCTACAAGACCATAT+TGG 0.485099 8.3:-79896720 MS.gene45370:CDS
AATAACATGACTTGTGTTGT+TGG 0.488088 8.3:+79897624 None:intergenic
GAGATGTAATACTAGAATAT+GGG 0.488938 8.3:-79896932 MS.gene45370:CDS
CCCTTTGAGTGTTCTTGAGG+AGG 0.493576 8.3:-79897482 MS.gene45370:CDS
CAAGGAAGCATGTGAAACTT+GGG 0.494320 8.3:-79897533 MS.gene45370:CDS
GCTCTCCAGCACTCAATTGG+AGG 0.521161 8.3:-79897348 MS.gene45370:CDS
CTATCCCTCCAATTGAGTGC+TGG 0.521685 8.3:+79897343 None:intergenic
AGGAATTGTTGACGATATCA+AGG 0.530296 8.3:-79897551 MS.gene45370:CDS
GAAACAAAAGCAGGTGTGAA+AGG 0.532657 8.3:-79897715 MS.gene45370:CDS
ACATTAACTATGAGAGCTCC+AGG 0.533138 8.3:+79896648 None:intergenic
CATATTTGTCTGGTTGATGG+TGG 0.543038 8.3:+79897653 None:intergenic
AATCCCTTTGAGTGTTCTTG+AGG 0.545874 8.3:-79897485 MS.gene45370:CDS
ACCTCCTCAAGAACACTCAA+AGG 0.547379 8.3:+79897481 None:intergenic
CCTCCTCAAGAACACTCAAA+GGG 0.551009 8.3:+79897482 None:intergenic
CCATCAACCAGACAAATATG+AGG 0.555026 8.3:-79897650 MS.gene45370:CDS
AGGTGAAAGATGGAGTGAAG+AGG 0.558342 8.3:-79897462 MS.gene45370:CDS
CAATTCCTTGATGAATACTT+GGG 0.576394 8.3:+79897566 None:intergenic
CTCTCCAGCACTCAATTGGA+GGG 0.578608 8.3:-79897347 MS.gene45370:CDS
CAAACATACCTGCATACTGC+GGG 0.579759 8.3:+79897274 None:intergenic
CAGGACAAGAAGGATAGTAA+TGG 0.586186 8.3:+79896796 None:intergenic
AATTCTGGTTCAGGACAAGA+AGG 0.588777 8.3:+79896786 None:intergenic
ATATCTTTAAGGTCGATGAC+AGG 0.590653 8.3:+79897601 None:intergenic
GTGAAAGGACTTGTTGATGC+AGG 0.590786 8.3:-79897700 MS.gene45370:CDS
GCATTTGATGAAACAAAAGC+AGG 0.595830 8.3:-79897724 MS.gene45370:CDS
AAAGACCCAAGTATTCATCA+AGG 0.600194 8.3:-79897571 MS.gene45370:CDS
GGAGCTCTCATAGTTAATGT+TGG 0.602821 8.3:-79896645 MS.gene45370:CDS
GGAAAATCTTCTGGTTTGGG+AGG 0.607287 8.3:+79897295 None:intergenic
GGTTGATGGTGGAATAAGCT+TGG 0.607514 8.3:+79897664 None:intergenic
AAGGAAGCATGTGAAACTTG+GGG 0.628246 8.3:-79897532 MS.gene45370:CDS
CTTCTACAAGACCATATTGG+TGG 0.634375 8.3:-79896717 MS.gene45370:CDS
ACAAACATACCTGCATACTG+CGG 0.682866 8.3:+79897273 None:intergenic
TCCTGAACCAGAATTGACTG+TGG 0.682984 8.3:-79896778 MS.gene45370:CDS
CCTGAACCAGAATTGACTGT+GGG 0.714206 8.3:-79896777 MS.gene45370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATAAAACAAATAAATATT+AGG - chr8.3:79897296-79897315 MS.gene45370:CDS 10.0%
!! CTTTATTATCAATATCTTTA+AGG + chr8.3:79896768-79896787 None:intergenic 15.0%
!!! AAAATTGCTATTGTAAATAA+AGG + chr8.3:79896977-79896996 None:intergenic 15.0%
!! AAGTTCTTTATGAAGATAAA+TGG - chr8.3:79897666-79897685 MS.gene45370:CDS 20.0%
!! ATTTATCTTCATAAAGAACT+TGG + chr8.3:79897667-79897686 None:intergenic 20.0%
!! GTAAGGATTAAATTCAATTT+CGG + chr8.3:79897361-79897380 None:intergenic 20.0%
!!! GTTTTATATTTTGTATCAGT+TGG + chr8.3:79897336-79897355 None:intergenic 20.0%
!!! TTTTTTCAAGTTGTTAATCA+TGG - chr8.3:79896847-79896866 MS.gene45370:CDS 20.0%
! AGAGATGTAATACTAGAATA+TGG - chr8.3:79897422-79897441 MS.gene45370:CDS 25.0%
! AGCAACAAAAAAAAACAGTA+AGG + chr8.3:79897378-79897397 None:intergenic 25.0%
! ATCTTATAAATAGATCTTCC+TGG + chr8.3:79897271-79897290 None:intergenic 25.0%
! GAGATGTAATACTAGAATAT+GGG - chr8.3:79897423-79897442 MS.gene45370:CDS 25.0%
!!! CACTTTAAGATGTGAAATTA+TGG - chr8.3:79897193-79897212 MS.gene45370:intron 25.0%
AATACTAGAATATGGGAAAC+AGG - chr8.3:79897430-79897449 MS.gene45370:CDS 30.0%
ACAATTCCTTGATGAATACT+TGG + chr8.3:79896793-79896812 None:intergenic 30.0%
CAATTCCTTGATGAATACTT+GGG + chr8.3:79896792-79896811 None:intergenic 30.0%
CACATCTTAAAGTGAATAAG+TGG + chr8.3:79897187-79897206 None:intergenic 30.0%
GCATGTGTGTAAAATTTATG+CGG - chr8.3:79897107-79897126 MS.gene45370:intron 30.0%
TATGGATATTGCAACATGTA+GGG - chr8.3:79897138-79897157 MS.gene45370:intron 30.0%
TCCAAACCATCTAAAAGATA+TGG - chr8.3:79897508-79897527 MS.gene45370:CDS 30.0%
! AAAGATATGGATTGTGCTAA+AGG - chr8.3:79897521-79897540 MS.gene45370:CDS 30.0%
! AATAACATGACTTGTGTTGT+TGG + chr8.3:79896734-79896753 None:intergenic 30.0%
! ATGAAATGCATATTTTCTCC+AGG - chr8.3:79897250-79897269 MS.gene45370:intron 30.0%
! CACAATCCATATCTTTTAGA+TGG + chr8.3:79897517-79897536 None:intergenic 30.0%
! TCCATATCTTTTAGATGGTT+TGG + chr8.3:79897512-79897531 None:intergenic 30.0%
!!! GGATAGTTTTAGTTGTTACT+TGG - chr8.3:79897029-79897048 MS.gene45370:intron 30.0%
AAAGACCCAAGTATTCATCA+AGG - chr8.3:79896784-79896803 MS.gene45370:CDS 35.0%
AGACTACTTGACTAAGAAGA+AGG - chr8.3:79897221-79897240 MS.gene45370:intron 35.0%
AGGAATTGTTGACGATATCA+AGG - chr8.3:79896804-79896823 MS.gene45370:CDS 35.0%
ATTGATATAACACCTGTTCC+TGG - chr8.3:79897689-79897708 MS.gene45370:CDS 35.0%
GTAAAATTTATGCGGTCAGT+TGG - chr8.3:79897115-79897134 MS.gene45370:intron 35.0%
GTATGGATATTGCAACATGT+AGG - chr8.3:79897137-79897156 MS.gene45370:intron 35.0%
!! ATATCTTTAAGGTCGATGAC+AGG + chr8.3:79896757-79896776 None:intergenic 35.0%
!! GAATTGATGTCAGAAGCATT+AGG - chr8.3:79897479-79897498 MS.gene45370:CDS 35.0%
AATCCCTTTGAGTGTTCTTG+AGG - chr8.3:79896870-79896889 MS.gene45370:CDS 40.0%
ACAAACATACCTGCATACTG+CGG + chr8.3:79897085-79897104 None:intergenic 40.0%
ACATTAACTATGAGAGCTCC+AGG + chr8.3:79897710-79897729 None:intergenic 40.0%
CAGGACAAGAAGGATAGTAA+TGG + chr8.3:79897562-79897581 None:intergenic 40.0%
CCATCAACCAGACAAATATG+AGG - chr8.3:79896705-79896724 MS.gene45370:CDS 40.0%
CCTCATATTTGTCTGGTTGA+TGG + chr8.3:79896708-79896727 None:intergenic 40.0%
CTTCTACAAGACCATATTGG+TGG - chr8.3:79897638-79897657 MS.gene45370:CDS 40.0%
GAAACAAAAGCAGGTGTGAA+AGG - chr8.3:79896640-79896659 MS.gene45370:CDS 40.0%
GGAGCTCTCATAGTTAATGT+TGG - chr8.3:79897710-79897729 MS.gene45370:CDS 40.0%
GTGCTTCTACAAGACCATAT+TGG - chr8.3:79897635-79897654 MS.gene45370:CDS 40.0%
TAGGGTTGTGGTTCAAATCT+GGG - chr8.3:79897156-79897175 MS.gene45370:intron 40.0%
TATTGCAACATGTAGGGTTG+TGG - chr8.3:79897144-79897163 MS.gene45370:intron 40.0%
TTGGCTTCCTCATATTTGTC+TGG + chr8.3:79896715-79896734 None:intergenic 40.0%
! AAGGAAGCATGTGAAACTTG+GGG - chr8.3:79896823-79896842 MS.gene45370:CDS 40.0%
! ATTTATGCGGTCAGTTGGTA+TGG - chr8.3:79897120-79897139 MS.gene45370:intron 40.0%
! CAAGGAAGCATGTGAAACTT+GGG - chr8.3:79896822-79896841 MS.gene45370:CDS 40.0%
! TCAAGGAAGCATGTGAAACT+TGG - chr8.3:79896821-79896840 MS.gene45370:CDS 40.0%
!! AATTCTGGTTCAGGACAAGA+AGG + chr8.3:79897572-79897591 None:intergenic 40.0%
!! CATATTTGTCTGGTTGATGG+TGG + chr8.3:79896705-79896724 None:intergenic 40.0%
ACCTCCTCAAGAACACTCAA+AGG + chr8.3:79896877-79896896 None:intergenic 45.0%
AGGTGAAAGATGGAGTGAAG+AGG - chr8.3:79896893-79896912 MS.gene45370:CDS 45.0%
ATAGCTCTCCAGCACTCAAT+TGG - chr8.3:79897004-79897023 MS.gene45370:intron 45.0%
CAAACATACCTGCATACTGC+GGG + chr8.3:79897084-79897103 None:intergenic 45.0%
CATACTGCGGGAAAATCTTC+TGG + chr8.3:79897072-79897091 None:intergenic 45.0%
CCTCCTCAAGAACACTCAAA+GGG + chr8.3:79896876-79896895 None:intergenic 45.0%
CCTGAACCAGAATTGACTGT+GGG - chr8.3:79897578-79897597 MS.gene45370:CDS 45.0%
GAACTTGGAGACCACCAATA+TGG + chr8.3:79897652-79897671 None:intergenic 45.0%
GGGAAACAGGTGATGAATCT+TGG - chr8.3:79897443-79897462 MS.gene45370:CDS 45.0%
GTAGGGTTGTGGTTCAAATC+TGG - chr8.3:79897155-79897174 MS.gene45370:intron 45.0%
GTTCTTGAGGAGGTGAAAGA+TGG - chr8.3:79896883-79896902 MS.gene45370:CDS 45.0%
GTTGTTCCCACAGTCAATTC+TGG + chr8.3:79897587-79897606 None:intergenic 45.0%
TCCTGAACCAGAATTGACTG+TGG - chr8.3:79897577-79897596 MS.gene45370:CDS 45.0%
! AAGATTTTCCCGCAGTATGC+AGG - chr8.3:79897073-79897092 MS.gene45370:intron 45.0%
! TGCGGGAAAATCTTCTGGTT+TGG + chr8.3:79897067-79897086 None:intergenic 45.0%
!! GCGGGAAAATCTTCTGGTTT+GGG + chr8.3:79897066-79897085 None:intergenic 45.0%
!! GGAAAATCTTCTGGTTTGGG+AGG + chr8.3:79897063-79897082 None:intergenic 45.0%
!! GGTTGATGGTGGAATAAGCT+TGG + chr8.3:79896694-79896713 None:intergenic 45.0%
!! GTGAAAGGACTTGTTGATGC+AGG - chr8.3:79896655-79896674 MS.gene45370:CDS 45.0%
ACTATGAGAGCTCCAGGAAC+AGG + chr8.3:79897704-79897723 None:intergenic 50.0%
CCCTTTGAGTGTTCTTGAGG+AGG - chr8.3:79896873-79896892 MS.gene45370:CDS 50.0%
CTATCCCTCCAATTGAGTGC+TGG + chr8.3:79897015-79897034 None:intergenic 50.0%
CTCTCCAGCACTCAATTGGA+GGG - chr8.3:79897008-79897027 MS.gene45370:intron 50.0%
!! CCCACAGTCAATTCTGGTTC+AGG + chr8.3:79897581-79897600 None:intergenic 50.0%
!! TCTTCTGGTTTGGGAGGATC+AGG + chr8.3:79897057-79897076 None:intergenic 50.0%
GCTCTCCAGCACTCAATTGG+AGG - chr8.3:79897007-79897026 MS.gene45370:intron 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 79896632 79897745 79896632 ID=MS.gene45370
chr8.3 mRNA 79896632 79897745 79896632 ID=MS.gene45370.t1;Parent=MS.gene45370
chr8.3 exon 79897283 79897745 79897283 ID=MS.gene45370.t1.exon1;Parent=MS.gene45370.t1
chr8.3 CDS 79897283 79897745 79897283 ID=cds.MS.gene45370.t1;Parent=MS.gene45370.t1
chr8.3 exon 79896632 79896953 79896632 ID=MS.gene45370.t1.exon2;Parent=MS.gene45370.t1
chr8.3 CDS 79896632 79896953 79896632 ID=cds.MS.gene45370.t1;Parent=MS.gene45370.t1
Gene Sequence

>MS.gene45370

CATTTGATGAAACAAAAGCAGGTGTGAAAGGACTTGTTGATGCAGGAGTTGAGAAGATACCAAGCTTATTCCACCATCAACCAGACAAATATGAGGAAGCCAACAACACAAGTCATGTTATTCCTGTCATCGACCTTAAAGATATTGATAATAAAGACCCAAGTATTCATCAAGGAATTGTTGACGATATCAAGGAAGCATGTGAAACTTGGGGTTTTTTTCAAGTTGTTAATCATGGAATCCCTTTGAGTGTTCTTGAGGAGGTGAAAGATGGAGTGAAGAGGTTTTATGAACAAGATACAGAAGTCAAAAAAAGTATGTATACAAGAGATTATCAGAGATCCTTTATTTACAATAGCAATTTTGATATTTATAGCTCTCCAGCACTCAATTGGAGGGATAGTTTTAGTTGTTACTTGGCTCCTGATCCTCCCAAACCAGAAGATTTTCCCGCAGTATGCAGGTATGTTTGTATGCATGTGTGTAAAATTTATGCGGTCAGTTGGTATGGATATTGCAACATGTAGGGTTGTGGTTCAAATCTGGGATTCTCCACTTATTCACTTTAAGATGTGAAATTATGGCTACTAGACTACTTGACTAAGAAGAAGGAAAAAAATGAAATGCATATTTTCTCCAGGAAGATCTATTTATAAGATACAAATCATAAAACAAATAAATATTAGGATAAAAACTAAAAACCAACTGATACAAAATATAAAACATCCGAAATTGAATTTAATCCTTACTGTTTTTTTTTGTTGCTTTCTAATTAGCTATTTATTCTTGCAGAGATGTAATACTAGAATATGGGAAACAGGTGATGAATCTTGGAACTTTACTGTTTGAATTGATGTCAGAAGCATTAGGTTTGAATCCAAACCATCTAAAAGATATGGATTGTGCTAAAGGACTGTTTGCTCTTTGCCATTACTATCCTTCTTGTCCTGAACCAGAATTGACTGTGGGAACAACAAAGCATTCTGATAATGATTTTCTCACAGTGCTTCTACAAGACCATATTGGTGGTCTCCAAGTTCTTTATGAAGATAAATGGATTGATATAACACCTGTTCCTGGAGCTCTCATAGTTAATGTTGGTGATCTTTTGCAG

Protein sequence

>MS.gene45370.t1

HLMKQKQVUKDLLMQELRRYQAYSTINQTNMRKPTTQVMLFLSSTLKILIIKTQVFIKELLTISRKHVKLGVFFKLLIMESLUVFLRRUKMEURGFMNKIQKSKKVCIQEIIRDPLFTIAILIFIALQHSIGGIVLVVTWLLILPNQKIFPQYAEMUYUNMGNRUUILELYCLNUCQKHUVUIQTIUKIWIVLKDCLLFAITILLVLNQNULWEQQSILIMIFSQCFYKTILVVSKFFMKINGLIUHLFLELSULMLVIFC