Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94547.t1 | XP_003631090.1 | 93.8 | 256 | 16 | 0 | 1 | 256 | 1 | 256 | 1.30E-117 | 432.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94547.t1 | Q9LDY5 | 69.4 | 186 | 53 | 1 | 75 | 256 | 62 | 247 | 4.9e-73 | 275.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94547.t1 | Q1SMZ8 | 93.8 | 256 | 16 | 0 | 1 | 256 | 1 | 256 | 9.4e-118 | 432.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene94547 | 0.808223 | 3.44E-50 | -1.69E-46 |
| MS.gene049171 | MS.gene94547 | 0.841292 | 5.19E-58 | -1.69E-46 |
| MS.gene049173 | MS.gene94547 | 0.839484 | 1.54E-57 | -1.69E-46 |
| MS.gene049217 | MS.gene94547 | 0.84123 | 5.39E-58 | -1.69E-46 |
| MS.gene049222 | MS.gene94547 | 0.858563 | 7.56E-63 | -1.69E-46 |
| MS.gene049285 | MS.gene94547 | 0.876054 | 1.89E-68 | -1.69E-46 |
| MS.gene049320 | MS.gene94547 | 0.882696 | 8.39E-71 | -1.69E-46 |
| MS.gene049427 | MS.gene94547 | 0.83189 | 1.29E-55 | -1.69E-46 |
| MS.gene049609 | MS.gene94547 | 0.821757 | 3.41E-53 | -1.69E-46 |
| MS.gene049805 | MS.gene94547 | 0.813915 | 2.01E-51 | -1.69E-46 |
| MS.gene050076 | MS.gene94547 | 0.80783 | 4.17E-50 | -1.69E-46 |
| MS.gene050163 | MS.gene94547 | 0.896998 | 2.15E-76 | -1.69E-46 |
| MS.gene050165 | MS.gene94547 | 0.889455 | 2.39E-73 | -1.69E-46 |
| MS.gene050333 | MS.gene94547 | 0.851344 | 9.44E-61 | -1.69E-46 |
| MS.gene050432 | MS.gene94547 | 0.82115 | 4.70E-53 | -1.69E-46 |
| MS.gene050444 | MS.gene94547 | 0.852384 | 4.78E-61 | -1.69E-46 |
| MS.gene050504 | MS.gene94547 | 0.846678 | 1.87E-59 | -1.69E-46 |
| MS.gene050507 | MS.gene94547 | 0.80815 | 3.57E-50 | -1.69E-46 |
| MS.gene050518 | MS.gene94547 | 0.858998 | 5.61E-63 | -1.69E-46 |
| MS.gene050538 | MS.gene94547 | 0.835404 | 1.71E-56 | -1.69E-46 |
| MS.gene050577 | MS.gene94547 | 0.831583 | 1.54E-55 | -1.69E-46 |
| MS.gene050612 | MS.gene94547 | 0.859825 | 3.16E-63 | -1.69E-46 |
| MS.gene050675 | MS.gene94547 | 0.829257 | 5.70E-55 | -1.69E-46 |
| MS.gene050699 | MS.gene94547 | 0.86661 | 2.51E-65 | -1.69E-46 |
| MS.gene050747 | MS.gene94547 | 0.813307 | 2.74E-51 | -1.69E-46 |
| MS.gene050767 | MS.gene94547 | 0.834455 | 2.97E-56 | -1.69E-46 |
| MS.gene050770 | MS.gene94547 | 0.838548 | 2.69E-57 | -1.69E-46 |
| MS.gene050772 | MS.gene94547 | 0.84412 | 9.20E-59 | -1.69E-46 |
| MS.gene050781 | MS.gene94547 | 0.823641 | 1.24E-53 | -1.69E-46 |
| MS.gene05080 | MS.gene94547 | 0.806889 | 6.61E-50 | -1.69E-46 |
| MS.gene050950 | MS.gene94547 | 0.810683 | 1.02E-50 | -1.69E-46 |
| MS.gene050986 | MS.gene94547 | 0.830012 | 3.73E-55 | -1.69E-46 |
| MS.gene05100 | MS.gene94547 | 0.881209 | 2.90E-70 | -1.69E-46 |
| MS.gene051228 | MS.gene94547 | 0.887313 | 1.59E-72 | -1.69E-46 |
| MS.gene051317 | MS.gene94547 | 0.828492 | 8.73E-55 | -1.69E-46 |
| MS.gene051551 | MS.gene94547 | 0.806904 | 6.56E-50 | -1.69E-46 |
| MS.gene051617 | MS.gene94547 | 0.834349 | 3.16E-56 | -1.69E-46 |
| MS.gene051666 | MS.gene94547 | 0.862478 | 4.93E-64 | -1.69E-46 |
| MS.gene051810 | MS.gene94547 | 0.890592 | 8.57E-74 | -1.69E-46 |
| MS.gene051858 | MS.gene94547 | 0.810456 | 1.14E-50 | -1.69E-46 |
| MS.gene051949 | MS.gene94547 | 0.879502 | 1.18E-69 | -1.69E-46 |
| MS.gene052050 | MS.gene94547 | -0.820066 | 8.35E-53 | -1.69E-46 |
| MS.gene052271 | MS.gene94547 | 0.852333 | 4.94E-61 | -1.69E-46 |
| MS.gene052290 | MS.gene94547 | 0.876243 | 1.63E-68 | -1.69E-46 |
| MS.gene052379 | MS.gene94547 | 0.864388 | 1.26E-64 | -1.69E-46 |
| MS.gene052615 | MS.gene94547 | 0.810432 | 1.16E-50 | -1.69E-46 |
| MS.gene052870 | MS.gene94547 | 0.813455 | 2.54E-51 | -1.69E-46 |
| MS.gene052872 | MS.gene94547 | 0.81858 | 1.82E-52 | -1.69E-46 |
| MS.gene053009 | MS.gene94547 | 0.852728 | 3.82E-61 | -1.69E-46 |
| MS.gene053103 | MS.gene94547 | 0.809709 | 1.65E-50 | -1.69E-46 |
| MS.gene053104 | MS.gene94547 | 0.847042 | 1.49E-59 | -1.69E-46 |
| MS.gene053107 | MS.gene94547 | 0.809102 | 2.23E-50 | -1.69E-46 |
| MS.gene053207 | MS.gene94547 | 0.835777 | 1.38E-56 | -1.69E-46 |
| MS.gene053213 | MS.gene94547 | 0.855909 | 4.60E-62 | -1.69E-46 |
| MS.gene053286 | MS.gene94547 | 0.853503 | 2.29E-61 | -1.69E-46 |
| MS.gene053322 | MS.gene94547 | 0.861456 | 1.01E-63 | -1.69E-46 |
| MS.gene053579 | MS.gene94547 | 0.802658 | 5.05E-49 | -1.69E-46 |
| MS.gene053632 | MS.gene94547 | 0.887553 | 1.29E-72 | -1.69E-46 |
| MS.gene053916 | MS.gene94547 | 0.833959 | 3.95E-56 | -1.69E-46 |
| MS.gene054376 | MS.gene94547 | 0.823713 | 1.19E-53 | -1.69E-46 |
| MS.gene054600 | MS.gene94547 | 0.86369 | 2.08E-64 | -1.69E-46 |
| MS.gene054642 | MS.gene94547 | 0.893045 | 9.05E-75 | -1.69E-46 |
| MS.gene05470 | MS.gene94547 | 0.822943 | 1.81E-53 | -1.69E-46 |
| MS.gene054802 | MS.gene94547 | 0.822001 | 2.99E-53 | -1.69E-46 |
| MS.gene054803 | MS.gene94547 | 0.818976 | 1.48E-52 | -1.69E-46 |
| MS.gene054804 | MS.gene94547 | 0.82553 | 4.46E-54 | -1.69E-46 |
| MS.gene054954 | MS.gene94547 | 0.83418 | 3.48E-56 | -1.69E-46 |
| MS.gene05505 | MS.gene94547 | 0.803671 | 3.12E-49 | -1.69E-46 |
| MS.gene055206 | MS.gene94547 | 0.820284 | 7.44E-53 | -1.69E-46 |
| MS.gene055212 | MS.gene94547 | 0.833147 | 6.30E-56 | -1.69E-46 |
| MS.gene055254 | MS.gene94547 | 0.861792 | 8.00E-64 | -1.69E-46 |
| MS.gene055289 | MS.gene94547 | 0.800264 | 1.56E-48 | -1.69E-46 |
| MS.gene055362 | MS.gene94547 | 0.864603 | 1.08E-64 | -1.69E-46 |
| MS.gene055397 | MS.gene94547 | 0.884361 | 2.05E-71 | -1.69E-46 |
| MS.gene055499 | MS.gene94547 | 0.86887 | 4.73E-66 | -1.69E-46 |
| MS.gene055502 | MS.gene94547 | 0.842242 | 2.91E-58 | -1.69E-46 |
| MS.gene055577 | MS.gene94547 | 0.842102 | 3.17E-58 | -1.69E-46 |
| MS.gene055647 | MS.gene94547 | 0.801589 | 8.38E-49 | -1.69E-46 |
| MS.gene055722 | MS.gene94547 | 0.81738 | 3.40E-52 | -1.69E-46 |
| MS.gene055748 | MS.gene94547 | 0.839718 | 1.34E-57 | -1.69E-46 |
| MS.gene055751 | MS.gene94547 | 0.833477 | 5.22E-56 | -1.69E-46 |
| MS.gene05592 | MS.gene94547 | 0.827956 | 1.17E-54 | -1.69E-46 |
| MS.gene055941 | MS.gene94547 | 0.818475 | 1.92E-52 | -1.69E-46 |
| MS.gene055956 | MS.gene94547 | -0.8336 | 4.86E-56 | -1.69E-46 |
| MS.gene056083 | MS.gene94547 | 0.838455 | 2.85E-57 | -1.69E-46 |
| MS.gene056374 | MS.gene94547 | 0.849922 | 2.37E-60 | -1.69E-46 |
| MS.gene056449 | MS.gene94547 | 0.804622 | 1.98E-49 | -1.69E-46 |
| MS.gene056487 | MS.gene94547 | 0.80883 | 2.55E-50 | -1.69E-46 |
| MS.gene056510 | MS.gene94547 | 0.81043 | 1.16E-50 | -1.69E-46 |
| MS.gene056609 | MS.gene94547 | 0.816808 | 4.57E-52 | -1.69E-46 |
| MS.gene056657 | MS.gene94547 | 0.883949 | 2.91E-71 | -1.69E-46 |
| MS.gene056692 | MS.gene94547 | 0.830283 | 3.20E-55 | -1.69E-46 |
| MS.gene056897 | MS.gene94547 | 0.831352 | 1.75E-55 | -1.69E-46 |
| MS.gene056903 | MS.gene94547 | 0.808501 | 3.00E-50 | -1.69E-46 |
| MS.gene057011 | MS.gene94547 | 0.804385 | 2.22E-49 | -1.69E-46 |
| MS.gene057129 | MS.gene94547 | 0.897819 | 9.71E-77 | -1.69E-46 |
| MS.gene057246 | MS.gene94547 | 0.910506 | 1.74E-82 | -1.69E-46 |
| MS.gene057295 | MS.gene94547 | 0.806389 | 8.42E-50 | -1.69E-46 |
| MS.gene057331 | MS.gene94547 | 0.818852 | 1.58E-52 | -1.69E-46 |
| MS.gene057332 | MS.gene94547 | 0.808517 | 2.98E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene94547 | 0.843233 | 1.59E-58 | -1.69E-46 |
| MS.gene057658 | MS.gene94547 | 0.835291 | 1.83E-56 | -1.69E-46 |
| MS.gene057831 | MS.gene94547 | 0.815167 | 1.06E-51 | -1.69E-46 |
| MS.gene057926 | MS.gene94547 | 0.808345 | 3.24E-50 | -1.69E-46 |
| MS.gene058019 | MS.gene94547 | 0.824489 | 7.85E-54 | -1.69E-46 |
| MS.gene058293 | MS.gene94547 | 0.809862 | 1.53E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene94547 | 0.885948 | 5.24E-72 | -1.69E-46 |
| MS.gene058544 | MS.gene94547 | 0.85659 | 2.90E-62 | -1.69E-46 |
| MS.gene058723 | MS.gene94547 | 0.902708 | 7.35E-79 | -1.69E-46 |
| MS.gene058748 | MS.gene94547 | 0.807896 | 4.04E-50 | -1.69E-46 |
| MS.gene058773 | MS.gene94547 | 0.848382 | 6.35E-60 | -1.69E-46 |
| MS.gene058838 | MS.gene94547 | 0.816399 | 5.64E-52 | -1.69E-46 |
| MS.gene059022 | MS.gene94547 | 0.84137 | 4.95E-58 | -1.69E-46 |
| MS.gene059023 | MS.gene94547 | 0.826518 | 2.60E-54 | -1.69E-46 |
| MS.gene059029 | MS.gene94547 | 0.842645 | 2.28E-58 | -1.69E-46 |
| MS.gene059079 | MS.gene94547 | 0.812945 | 3.28E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene94547 | -0.810429 | 1.16E-50 | -1.69E-46 |
| MS.gene05913 | MS.gene94547 | 0.810121 | 1.35E-50 | -1.69E-46 |
| MS.gene05914 | MS.gene94547 | 0.829212 | 5.84E-55 | -1.69E-46 |
| MS.gene05934 | MS.gene94547 | 0.843707 | 1.19E-58 | -1.69E-46 |
| MS.gene059536 | MS.gene94547 | 0.817944 | 2.54E-52 | -1.69E-46 |
| MS.gene059677 | MS.gene94547 | 0.894959 | 1.51E-75 | -1.69E-46 |
| MS.gene059718 | MS.gene94547 | 0.865702 | 4.87E-65 | -1.69E-46 |
| MS.gene059756 | MS.gene94547 | 0.820828 | 5.58E-53 | -1.69E-46 |
| MS.gene059858 | MS.gene94547 | 0.859471 | 4.04E-63 | -1.69E-46 |
| MS.gene059865 | MS.gene94547 | 0.805823 | 1.11E-49 | -1.69E-46 |
| MS.gene059902 | MS.gene94547 | 0.808886 | 2.49E-50 | -1.69E-46 |
| MS.gene059960 | MS.gene94547 | 0.878814 | 2.07E-69 | -1.69E-46 |
| MS.gene060341 | MS.gene94547 | 0.802752 | 4.83E-49 | -1.69E-46 |
| MS.gene060703 | MS.gene94547 | 0.803937 | 2.75E-49 | -1.69E-46 |
| MS.gene060728 | MS.gene94547 | 0.802532 | 5.37E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene94547 | 0.846736 | 1.80E-59 | -1.69E-46 |
| MS.gene060804 | MS.gene94547 | 0.829683 | 4.49E-55 | -1.69E-46 |
| MS.gene06092 | MS.gene94547 | 0.842409 | 2.63E-58 | -1.69E-46 |
| MS.gene061041 | MS.gene94547 | 0.835043 | 2.11E-56 | -1.69E-46 |
| MS.gene061150 | MS.gene94547 | 0.874241 | 7.88E-68 | -1.69E-46 |
| MS.gene061200 | MS.gene94547 | 0.858153 | 1.00E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94547.t1 | MTR_8g107010 | 98.047 | 256 | 5 | 0 | 1 | 256 | 1 | 256 | 0.0 | 509 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94547.t1 | AT1G18170 | 70.430 | 186 | 51 | 2 | 75 | 256 | 62 | 247 | 1.15e-95 | 281 |
| MS.gene94547.t1 | AT1G73655 | 50.000 | 252 | 100 | 4 | 1 | 251 | 1 | 227 | 1.50e-79 | 239 |
| MS.gene94547.t1 | AT3G10060 | 29.379 | 177 | 101 | 5 | 87 | 255 | 60 | 220 | 7.03e-13 | 66.6 |
Find 79 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGAGAATGGTGCTGATTT+AGG | 0.153499 | 4.1:-447676 | MS.gene94547:CDS |
| GATGAAACAACTGGTTTAAT+AGG | 0.212922 | 4.1:+448332 | None:intergenic |
| CTAAACATGGCCACTATCTT+TGG | 0.258527 | 4.1:-448530 | None:intergenic |
| GAGAGAATGGTGCTGATTTA+GGG | 0.288158 | 4.1:-447675 | MS.gene94547:CDS |
| AGTTATTGTGCCTCCTGAAT+TGG | 0.295641 | 4.1:-447704 | MS.gene94547:CDS |
| TTTGGTTGTGATTGATATAA+TGG | 0.298752 | 4.1:-447881 | MS.gene94547:CDS |
| TTGACTAGACGGTTTGGCCT+TGG | 0.356922 | 4.1:-448272 | MS.gene94547:CDS |
| TTCTCTCTAAATCCCAATTC+AGG | 0.369157 | 4.1:+447691 | None:intergenic |
| GAAACCATTGGCTCTAGTTA+TGG | 0.370613 | 4.1:-447806 | MS.gene94547:CDS |
| AAACCATTGGCTCTAGTTAT+GGG | 0.372920 | 4.1:-447805 | MS.gene94547:CDS |
| ATAATTAACCTTATGCCATT+AGG | 0.374600 | 4.1:+448022 | None:intergenic |
| TCACACACACCCTTACTATA+TGG | 0.378280 | 4.1:+447775 | None:intergenic |
| GTGATGACTGGTTCTGGTAT+GGG | 0.394646 | 4.1:+448484 | None:intergenic |
| GCCTTGGAGCTGGTCTTGCA+TGG | 0.396282 | 4.1:-448256 | MS.gene94547:CDS |
| ACCAGTTGTTTCATCAACTA+AGG | 0.400326 | 4.1:-448324 | MS.gene94547:CDS |
| GTTATTGTGCCTCCTGAATT+GGG | 0.401025 | 4.1:-447703 | MS.gene94547:CDS |
| TGTGATGACTGGTTCTGGTA+TGG | 0.408947 | 4.1:+448483 | None:intergenic |
| GTTGTTGACTGTGGAGATAA+TGG | 0.413539 | 4.1:+448410 | None:intergenic |
| TATGCCAAGGCGAGGTGATT+TGG | 0.421986 | 4.1:-447899 | MS.gene94547:CDS |
| CCTTGGAGCTGGTCTTGCAT+GGG | 0.424931 | 4.1:-448255 | MS.gene94547:CDS |
| TTGGTTGTGATTGATATAAT+GGG | 0.427668 | 4.1:-447880 | MS.gene94547:CDS |
| GAATGTTGAGGAGGAGAAAG+AGG | 0.427854 | 4.1:-448053 | MS.gene94547:CDS |
| AGGAATCTGTTGCTGTGCTT+TGG | 0.442859 | 4.1:+448352 | None:intergenic |
| TGATGACTGGTTCTGGTATG+GGG | 0.448826 | 4.1:+448485 | None:intergenic |
| ACCTTAGTTGATGAAACAAC+TGG | 0.450082 | 4.1:+448323 | None:intergenic |
| AGTGATGTGATGACTGGTTC+TGG | 0.451572 | 4.1:+448478 | None:intergenic |
| GGGGTCAAGGCCATATAGTA+AGG | 0.452971 | 4.1:-447785 | MS.gene94547:CDS |
| AGACGGTTTGGCCTTGGAGC+TGG | 0.454780 | 4.1:-448266 | MS.gene94547:CDS |
| GGAGCTGGTCTTGCATGGGT+TGG | 0.455263 | 4.1:-448251 | MS.gene94547:CDS |
| AGGTACTATGAATTGAAAAT+CGG | 0.465928 | 4.1:-447931 | MS.gene94547:CDS |
| AAAGAGGTGATTCTGCCTAA+TGG | 0.469758 | 4.1:-448037 | MS.gene94547:CDS |
| ACTTCCTTGACTAGACGGTT+TGG | 0.475249 | 4.1:-448278 | MS.gene94547:CDS |
| GTGTTTGTGAACACATTTGA+AGG | 0.494203 | 4.1:-447835 | MS.gene94547:CDS |
| CACAACAAGAATCTAATACA+AGG | 0.494667 | 4.1:-448175 | MS.gene94547:intron |
| GGTTGCGGTTGTTGTGGTGG+AGG | 0.494679 | 4.1:+448440 | None:intergenic |
| GAATTGGGATTTAGAGAGAA+TGG | 0.496442 | 4.1:-447688 | MS.gene94547:CDS |
| AACACTTGAATACGTTGTAC+AGG | 0.502708 | 4.1:-447626 | MS.gene94547:CDS |
| GAGGGTTGCGGTTGTTGTGG+TGG | 0.510547 | 4.1:+448437 | None:intergenic |
| CCTGAGGGTTGCGGTTGTTG+TGG | 0.518773 | 4.1:+448434 | None:intergenic |
| TGATTCTGCCTAATGGCATA+AGG | 0.521446 | 4.1:-448030 | MS.gene94547:intron |
| TTCAAATTTGTTGTTGACTG+TGG | 0.526248 | 4.1:+448401 | None:intergenic |
| AATGGTGCTGATTTAGGGTC+TGG | 0.527304 | 4.1:-447670 | MS.gene94547:CDS |
| ATGGGAAAGGTTGAAAGCAC+AGG | 0.529021 | 4.1:-447862 | MS.gene94547:CDS |
| TGATGAGGAACCAAAGATAG+TGG | 0.529530 | 4.1:+448520 | None:intergenic |
| CCACAACAACCGCAACCCTC+AGG | 0.533605 | 4.1:-448434 | MS.gene94547:CDS |
| TTTAATCAAAATCATCAAGC+AGG | 0.537963 | 4.1:+447583 | None:intergenic |
| TTGCCACTTCCTTGACTAGA+CGG | 0.539954 | 4.1:-448283 | MS.gene94547:CDS |
| CTATGAAGGCAGGAGGGAAG+AGG | 0.543711 | 4.1:-447729 | MS.gene94547:CDS |
| TATAGTAAGGGTGTGTGTGA+AGG | 0.554244 | 4.1:-447772 | MS.gene94547:CDS |
| ATAGTAAGGGTGTGTGTGAA+GGG | 0.556262 | 4.1:-447771 | MS.gene94547:CDS |
| AAATCATCAAGCAGGTGCAA+TGG | 0.556694 | 4.1:+447591 | None:intergenic |
| TTGTATTCATAGGAATGTTG+AGG | 0.558846 | 4.1:-448065 | MS.gene94547:intron |
| TGGCTCTAGTTATGGGGTCA+AGG | 0.567583 | 4.1:-447798 | MS.gene94547:CDS |
| TGAAGGAGACAAGAAACCAT+TGG | 0.568456 | 4.1:-447818 | MS.gene94547:CDS |
| CTAAGGTTGATTCCACAGAC+TGG | 0.569193 | 4.1:-448307 | MS.gene94547:CDS |
| TACTATGAATTGAAAATCGG+TGG | 0.577492 | 4.1:-447928 | MS.gene94547:CDS |
| AAACCGTCTAGTCAAGGAAG+TGG | 0.580205 | 4.1:+448280 | None:intergenic |
| GACCCCATAACTAGAGCCAA+TGG | 0.581524 | 4.1:+447802 | None:intergenic |
| GATAATGGACCTGAGGGTTG+CGG | 0.588310 | 4.1:+448425 | None:intergenic |
| TATTCATAGGAATGTTGAGG+AGG | 0.588540 | 4.1:-448062 | MS.gene94547:intron |
| TGTGATTGATATAATGGGAA+AGG | 0.597131 | 4.1:-447875 | MS.gene94547:CDS |
| GATGACTGGTTCTGGTATGG+GGG | 0.597654 | 4.1:+448486 | None:intergenic |
| ACAACCAAATCACCTCGCCT+TGG | 0.600709 | 4.1:+447895 | None:intergenic |
| AAGGCCAAACCGTCTAGTCA+AGG | 0.607001 | 4.1:+448274 | None:intergenic |
| TATTCAAGTGTTGCAAGTGG+AGG | 0.610335 | 4.1:+447637 | None:intergenic |
| CCCATGCAAGACCAGCTCCA+AGG | 0.610919 | 4.1:+448255 | None:intergenic |
| GGAAGTGGCAATCCAGTCTG+TGG | 0.620097 | 4.1:+448295 | None:intergenic |
| GGGTCAAGGCCATATAGTAA+GGG | 0.620171 | 4.1:-447784 | MS.gene94547:CDS |
| TCGGTGGTGGTGATATGCCA+AGG | 0.624769 | 4.1:-447912 | MS.gene94547:CDS |
| TGAAGAAGTGATGTGATGAC+TGG | 0.628381 | 4.1:+448472 | None:intergenic |
| AACCATTGGCTCTAGTTATG+GGG | 0.638329 | 4.1:-447804 | MS.gene94547:CDS |
| ACGTATTCAAGTGTTGCAAG+TGG | 0.645589 | 4.1:+447634 | None:intergenic |
| TATGAATTGAAAATCGGTGG+TGG | 0.648406 | 4.1:-447925 | MS.gene94547:CDS |
| CTGTGGAGATAATGGACCTG+AGG | 0.664299 | 4.1:+448418 | None:intergenic |
| GGGGAGTAAGAAAATTGATG+AGG | 0.664361 | 4.1:+448505 | None:intergenic |
| TCTCTAAATCCCAATTCAGG+AGG | 0.664404 | 4.1:+447694 | None:intergenic |
| TGCTGATTTAGGGTCTGGTG+TGG | 0.665665 | 4.1:-447665 | MS.gene94547:CDS |
| TGTGGAGATAATGGACCTGA+GGG | 0.682825 | 4.1:+448419 | None:intergenic |
| GGTGGTGATATGCCAAGGCG+AGG | 0.718124 | 4.1:-447907 | MS.gene94547:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATCAAAATCATATATGAA+TGG | - | chr4.1:448007-448026 | MS.gene94547:intron | 15.0% |
| !!! | AATGATATTTTTGTATTCAT+AGG | - | chr4.1:448046-448065 | MS.gene94547:CDS | 15.0% |
| !! | ATATGTACTAAAAACTATGA+AGG | - | chr4.1:448378-448397 | MS.gene94547:CDS | 20.0% |
| ! | AGGTACTATGAATTGAAAAT+CGG | - | chr4.1:448190-448209 | MS.gene94547:CDS | 25.0% |
| ! | ATAATTAACCTTATGCCATT+AGG | + | chr4.1:448102-448121 | None:intergenic | 25.0% |
| ! | TCTCAGAACAAATAAAAACT+CGG | - | chr4.1:447913-447932 | MS.gene94547:CDS | 25.0% |
| ! | TTGGTTGTGATTGATATAAT+GGG | - | chr4.1:448241-448260 | MS.gene94547:CDS | 25.0% |
| ! | TTTGGTTGTGATTGATATAA+TGG | - | chr4.1:448240-448259 | MS.gene94547:CDS | 25.0% |
| CACAACAAGAATCTAATACA+AGG | - | chr4.1:447946-447965 | MS.gene94547:intron | 30.0% | |
| TACTATGAATTGAAAATCGG+TGG | - | chr4.1:448193-448212 | MS.gene94547:CDS | 30.0% | |
| TTGTATTCATAGGAATGTTG+AGG | - | chr4.1:448056-448075 | MS.gene94547:CDS | 30.0% | |
| ! | GATGAAACAACTGGTTTAAT+AGG | + | chr4.1:447792-447811 | None:intergenic | 30.0% |
| ! | TGTGATTGATATAATGGGAA+AGG | - | chr4.1:448246-448265 | MS.gene94547:CDS | 30.0% |
| !! | TTCAAATTTGTTGTTGACTG+TGG | + | chr4.1:447723-447742 | None:intergenic | 30.0% |
| AACACTTGAATACGTTGTAC+AGG | - | chr4.1:448495-448514 | MS.gene94547:CDS | 35.0% | |
| GAATTGGGATTTAGAGAGAA+TGG | - | chr4.1:448433-448452 | MS.gene94547:CDS | 35.0% | |
| GTACTAAAAACTATGAAGGC+AGG | - | chr4.1:448382-448401 | MS.gene94547:CDS | 35.0% | |
| GTGTTTGTGAACACATTTGA+AGG | - | chr4.1:448286-448305 | MS.gene94547:CDS | 35.0% | |
| TAAAAACTATGAAGGCAGGA+GGG | - | chr4.1:448386-448405 | MS.gene94547:CDS | 35.0% | |
| TATGAATTGAAAATCGGTGG+TGG | - | chr4.1:448196-448215 | MS.gene94547:CDS | 35.0% | |
| TATTCATAGGAATGTTGAGG+AGG | - | chr4.1:448059-448078 | MS.gene94547:intron | 35.0% | |
| TTCTCTCTAAATCCCAATTC+AGG | + | chr4.1:448433-448452 | None:intergenic | 35.0% | |
| ! | ACAAATAAAAACTCGGCTTG+AGG | - | chr4.1:447920-447939 | MS.gene94547:CDS | 35.0% |
| ! | ACCAGTTGTTTCATCAACTA+AGG | - | chr4.1:447797-447816 | MS.gene94547:CDS | 35.0% |
| !! | AAACCATTGGCTCTAGTTAT+GGG | - | chr4.1:448316-448335 | MS.gene94547:CDS | 35.0% |
| !! | ACCTTAGTTGATGAAACAAC+TGG | + | chr4.1:447801-447820 | None:intergenic | 35.0% |
| AAAGAGGTGATTCTGCCTAA+TGG | - | chr4.1:448084-448103 | MS.gene94547:intron | 40.0% | |
| AGTTATTGTGCCTCCTGAAT+TGG | - | chr4.1:448417-448436 | MS.gene94547:CDS | 40.0% | |
| CTAAAAACTATGAAGGCAGG+AGG | - | chr4.1:448385-448404 | MS.gene94547:CDS | 40.0% | |
| GGGGAGTAAGAAAATTGATG+AGG | + | chr4.1:447619-447638 | None:intergenic | 40.0% | |
| GTTATTGTGCCTCCTGAATT+GGG | - | chr4.1:448418-448437 | MS.gene94547:CDS | 40.0% | |
| TCACACACACCCTTACTATA+TGG | + | chr4.1:448349-448368 | None:intergenic | 40.0% | |
| TCTCTAAATCCCAATTCAGG+AGG | + | chr4.1:448430-448449 | None:intergenic | 40.0% | |
| TGAAGAAGTGATGTGATGAC+TGG | + | chr4.1:447652-447671 | None:intergenic | 40.0% | |
| TGAAGGAGACAAGAAACCAT+TGG | - | chr4.1:448303-448322 | MS.gene94547:CDS | 40.0% | |
| TGATGAGGAACCAAAGATAG+TGG | + | chr4.1:447604-447623 | None:intergenic | 40.0% | |
| TGATTCTGCCTAATGGCATA+AGG | - | chr4.1:448091-448110 | MS.gene94547:intron | 40.0% | |
| ! | ACGTATTCAAGTGTTGCAAG+TGG | + | chr4.1:448490-448509 | None:intergenic | 40.0% |
| ! | ATAGTAAGGGTGTGTGTGAA+GGG | - | chr4.1:448350-448369 | MS.gene94547:CDS | 40.0% |
| ! | GTTGTTGACTGTGGAGATAA+TGG | + | chr4.1:447714-447733 | None:intergenic | 40.0% |
| ! | TATAGTAAGGGTGTGTGTGA+AGG | - | chr4.1:448349-448368 | MS.gene94547:CDS | 40.0% |
| ! | TATTCAAGTGTTGCAAGTGG+AGG | + | chr4.1:448487-448506 | None:intergenic | 40.0% |
| !! | AACCATTGGCTCTAGTTATG+GGG | - | chr4.1:448317-448336 | MS.gene94547:CDS | 40.0% |
| !! | AGAGAGAATGGTGCTGATTT+AGG | - | chr4.1:448445-448464 | MS.gene94547:CDS | 40.0% |
| !! | CGACTCATTTTTGTCTCTAC+AGG | - | chr4.1:448170-448189 | MS.gene94547:intron | 40.0% |
| !! | GAAACCATTGGCTCTAGTTA+TGG | - | chr4.1:448315-448334 | MS.gene94547:CDS | 40.0% |
| !! | GAGAGAATGGTGCTGATTTA+GGG | - | chr4.1:448446-448465 | MS.gene94547:CDS | 40.0% |
| !!! | TGGGTTGGTTTTCTTGCATT+TGG | - | chr4.1:447885-447904 | MS.gene94547:CDS | 40.0% |
| AAACCGTCTAGTCAAGGAAG+TGG | + | chr4.1:447844-447863 | None:intergenic | 45.0% | |
| ACTTCCTTGACTAGACGGTT+TGG | - | chr4.1:447843-447862 | MS.gene94547:CDS | 45.0% | |
| AGGAATCTGTTGCTGTGCTT+TGG | + | chr4.1:447772-447791 | None:intergenic | 45.0% | |
| GAATGTTGAGGAGGAGAAAG+AGG | - | chr4.1:448068-448087 | MS.gene94547:intron | 45.0% | |
| GGGTCAAGGCCATATAGTAA+GGG | - | chr4.1:448337-448356 | MS.gene94547:CDS | 45.0% | |
| ! | AGTGATGTGATGACTGGTTC+TGG | + | chr4.1:447646-447665 | None:intergenic | 45.0% |
| ! | ATGGGAAAGGTTGAAAGCAC+AGG | - | chr4.1:448259-448278 | MS.gene94547:CDS | 45.0% |
| ! | CTAAGGTTGATTCCACAGAC+TGG | - | chr4.1:447814-447833 | MS.gene94547:CDS | 45.0% |
| ! | TTGCCACTTCCTTGACTAGA+CGG | - | chr4.1:447838-447857 | MS.gene94547:CDS | 45.0% |
| !! | AATGGTGCTGATTTAGGGTC+TGG | - | chr4.1:448451-448470 | MS.gene94547:CDS | 45.0% |
| !! | GTGATGACTGGTTCTGGTAT+GGG | + | chr4.1:447640-447659 | None:intergenic | 45.0% |
| !! | TGATGACTGGTTCTGGTATG+GGG | + | chr4.1:447639-447658 | None:intergenic | 45.0% |
| !! | TGTGATGACTGGTTCTGGTA+TGG | + | chr4.1:447641-447660 | None:intergenic | 45.0% |
| !! | TGTGGAGATAATGGACCTGA+GGG | + | chr4.1:447705-447724 | None:intergenic | 45.0% |
| AAGGCCAAACCGTCTAGTCA+AGG | + | chr4.1:447850-447869 | None:intergenic | 50.0% | |
| ACAACCAAATCACCTCGCCT+TGG | + | chr4.1:448229-448248 | None:intergenic | 50.0% | |
| GACCCCATAACTAGAGCCAA+TGG | + | chr4.1:448322-448341 | None:intergenic | 50.0% | |
| GGGGTCAAGGCCATATAGTA+AGG | - | chr4.1:448336-448355 | MS.gene94547:CDS | 50.0% | |
| TATGCCAAGGCGAGGTGATT+TGG | - | chr4.1:448222-448241 | MS.gene94547:CDS | 50.0% | |
| ! | TTGACTAGACGGTTTGGCCT+TGG | - | chr4.1:447849-447868 | MS.gene94547:CDS | 50.0% |
| !! | CTGTGGAGATAATGGACCTG+AGG | + | chr4.1:447706-447725 | None:intergenic | 50.0% |
| !! | GATAATGGACCTGAGGGTTG+CGG | + | chr4.1:447699-447718 | None:intergenic | 50.0% |
| !! | GATGACTGGTTCTGGTATGG+GGG | + | chr4.1:447638-447657 | None:intergenic | 50.0% |
| !! | TGCTGATTTAGGGTCTGGTG+TGG | - | chr4.1:448456-448475 | MS.gene94547:CDS | 50.0% |
| !! | TGGCTCTAGTTATGGGGTCA+AGG | - | chr4.1:448323-448342 | MS.gene94547:CDS | 50.0% |
| CTATGAAGGCAGGAGGGAAG+AGG | - | chr4.1:448392-448411 | MS.gene94547:CDS | 55.0% | |
| GGAAGTGGCAATCCAGTCTG+TGG | + | chr4.1:447829-447848 | None:intergenic | 55.0% | |
| ! | TCGGTGGTGGTGATATGCCA+AGG | - | chr4.1:448209-448228 | MS.gene94547:CDS | 55.0% |
| !! | CCTTGGAGCTGGTCTTGCAT+GGG | - | chr4.1:447866-447885 | MS.gene94547:CDS | 55.0% |
| CCACAACAACCGCAACCCTC+AGG | - | chr4.1:447687-447706 | MS.gene94547:CDS | 60.0% | |
| CCCATGCAAGACCAGCTCCA+AGG | + | chr4.1:447869-447888 | None:intergenic | 60.0% | |
| CCTGAGGGTTGCGGTTGTTG+TGG | + | chr4.1:447690-447709 | None:intergenic | 60.0% | |
| GAGGGTTGCGGTTGTTGTGG+TGG | + | chr4.1:447687-447706 | None:intergenic | 60.0% | |
| GGTGGTGATATGCCAAGGCG+AGG | - | chr4.1:448214-448233 | MS.gene94547:CDS | 60.0% | |
| GGTTGCGGTTGTTGTGGTGG+AGG | + | chr4.1:447684-447703 | None:intergenic | 60.0% | |
| !! | AGACGGTTTGGCCTTGGAGC+TGG | - | chr4.1:447855-447874 | MS.gene94547:CDS | 60.0% |
| !! | GCCTTGGAGCTGGTCTTGCA+TGG | - | chr4.1:447865-447884 | MS.gene94547:CDS | 60.0% |
| !! | GGAGCTGGTCTTGCATGGGT+TGG | - | chr4.1:447870-447889 | MS.gene94547:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 447597 | 448546 | 447597 | ID=MS.gene94547 |
| chr4.1 | mRNA | 447597 | 448546 | 447597 | ID=MS.gene94547.t1;Parent=MS.gene94547 |
| chr4.1 | exon | 448176 | 448546 | 448176 | ID=MS.gene94547.t1.exon1;Parent=MS.gene94547.t1 |
| chr4.1 | CDS | 448176 | 448546 | 448176 | ID=cds.MS.gene94547.t1;Parent=MS.gene94547.t1 |
| chr4.1 | exon | 448031 | 448075 | 448031 | ID=MS.gene94547.t1.exon2;Parent=MS.gene94547.t1 |
| chr4.1 | CDS | 448031 | 448075 | 448031 | ID=cds.MS.gene94547.t1;Parent=MS.gene94547.t1 |
| chr4.1 | exon | 447597 | 447951 | 447597 | ID=MS.gene94547.t1.exon3;Parent=MS.gene94547.t1 |
| chr4.1 | CDS | 447597 | 447951 | 447597 | ID=cds.MS.gene94547.t1;Parent=MS.gene94547.t1 |
| Gene Sequence |
| Protein sequence |
