Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68946.t1 | XP_003610460.1 | 95.6 | 295 | 7 | 1 | 1 | 295 | 1 | 289 | 4.20E-153 | 550.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68946.t1 | Q5E930 | 51.0 | 302 | 129 | 5 | 27 | 320 | 12 | 302 | 6.4e-70 | 265.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68946.t1 | G7JJA0 | 95.6 | 295 | 7 | 1 | 1 | 295 | 1 | 289 | 3.0e-153 | 550.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene68946 | -0.873005 | 2.05E-67 | -1.69E-46 |
| MS.gene049217 | MS.gene68946 | -0.831098 | 2.02E-55 | -1.69E-46 |
| MS.gene049222 | MS.gene68946 | -0.84706 | 1.47E-59 | -1.69E-46 |
| MS.gene049285 | MS.gene68946 | -0.847101 | 1.43E-59 | -1.69E-46 |
| MS.gene049320 | MS.gene68946 | -0.841112 | 5.79E-58 | -1.69E-46 |
| MS.gene04939 | MS.gene68946 | -0.809478 | 1.86E-50 | -1.69E-46 |
| MS.gene049611 | MS.gene68946 | 0.816618 | 5.04E-52 | -1.69E-46 |
| MS.gene049633 | MS.gene68946 | -0.873126 | 1.87E-67 | -1.69E-46 |
| MS.gene049634 | MS.gene68946 | 0.82343 | 1.39E-53 | -1.69E-46 |
| MS.gene049805 | MS.gene68946 | -0.811371 | 7.24E-51 | -1.69E-46 |
| MS.gene050163 | MS.gene68946 | -0.842691 | 2.22E-58 | -1.69E-46 |
| MS.gene050165 | MS.gene68946 | -0.845024 | 5.26E-59 | -1.69E-46 |
| MS.gene050170 | MS.gene68946 | -0.831609 | 1.51E-55 | -1.69E-46 |
| MS.gene050323 | MS.gene68946 | -0.847717 | 9.69E-60 | -1.69E-46 |
| MS.gene050333 | MS.gene68946 | -0.838798 | 2.32E-57 | -1.69E-46 |
| MS.gene050338 | MS.gene68946 | 0.836791 | 7.61E-57 | -1.69E-46 |
| MS.gene050381 | MS.gene68946 | 0.805333 | 1.40E-49 | -1.69E-46 |
| MS.gene050444 | MS.gene68946 | -0.891269 | 4.64E-74 | -1.69E-46 |
| MS.gene050496 | MS.gene68946 | -0.839892 | 1.21E-57 | -1.69E-46 |
| MS.gene050504 | MS.gene68946 | -0.859582 | 3.74E-63 | -1.69E-46 |
| MS.gene050518 | MS.gene68946 | -0.825159 | 5.46E-54 | -1.69E-46 |
| MS.gene050577 | MS.gene68946 | -0.851672 | 7.62E-61 | -1.69E-46 |
| MS.gene050612 | MS.gene68946 | -0.880181 | 6.78E-70 | -1.69E-46 |
| MS.gene050675 | MS.gene68946 | -0.855149 | 7.66E-62 | -1.69E-46 |
| MS.gene050699 | MS.gene68946 | -0.846134 | 2.63E-59 | -1.69E-46 |
| MS.gene050710 | MS.gene68946 | 0.805025 | 1.63E-49 | -1.69E-46 |
| MS.gene050747 | MS.gene68946 | -0.806678 | 7.32E-50 | -1.69E-46 |
| MS.gene050772 | MS.gene68946 | -0.803937 | 2.75E-49 | -1.69E-46 |
| MS.gene05079 | MS.gene68946 | 0.818271 | 2.14E-52 | -1.69E-46 |
| MS.gene050809 | MS.gene68946 | 0.838662 | 2.52E-57 | -1.69E-46 |
| MS.gene05080 | MS.gene68946 | -0.861196 | 1.22E-63 | -1.69E-46 |
| MS.gene05081 | MS.gene68946 | -0.810377 | 1.19E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene68946 | -0.860843 | 1.56E-63 | -1.69E-46 |
| MS.gene050871 | MS.gene68946 | -0.856475 | 3.14E-62 | -1.69E-46 |
| MS.gene050904 | MS.gene68946 | -0.841577 | 4.37E-58 | -1.69E-46 |
| MS.gene050950 | MS.gene68946 | -0.802146 | 6.44E-49 | -1.69E-46 |
| MS.gene050986 | MS.gene68946 | -0.827184 | 1.80E-54 | -1.69E-46 |
| MS.gene051044 | MS.gene68946 | 0.806856 | 6.71E-50 | -1.69E-46 |
| MS.gene05104 | MS.gene68946 | 0.832059 | 1.17E-55 | -1.69E-46 |
| MS.gene051138 | MS.gene68946 | 0.855446 | 6.28E-62 | -1.69E-46 |
| MS.gene051228 | MS.gene68946 | -0.813674 | 2.27E-51 | -1.69E-46 |
| MS.gene051268 | MS.gene68946 | -0.817269 | 3.60E-52 | -1.69E-46 |
| MS.gene051317 | MS.gene68946 | -0.864657 | 1.04E-64 | -1.69E-46 |
| MS.gene051517 | MS.gene68946 | 0.806146 | 9.48E-50 | -1.69E-46 |
| MS.gene051617 | MS.gene68946 | -0.819547 | 1.10E-52 | -1.69E-46 |
| MS.gene051632 | MS.gene68946 | 0.80247 | 5.53E-49 | -1.69E-46 |
| MS.gene051651 | MS.gene68946 | 0.85305 | 3.09E-61 | -1.69E-46 |
| MS.gene051666 | MS.gene68946 | -0.810509 | 1.11E-50 | -1.69E-46 |
| MS.gene051749 | MS.gene68946 | 0.805697 | 1.18E-49 | -1.69E-46 |
| MS.gene051810 | MS.gene68946 | -0.822626 | 2.14E-53 | -1.69E-46 |
| MS.gene051838 | MS.gene68946 | -0.839614 | 1.43E-57 | -1.69E-46 |
| MS.gene051874 | MS.gene68946 | -0.830085 | 3.58E-55 | -1.69E-46 |
| MS.gene051907 | MS.gene68946 | 0.823384 | 1.43E-53 | -1.69E-46 |
| MS.gene051949 | MS.gene68946 | -0.838846 | 2.26E-57 | -1.69E-46 |
| MS.gene051973 | MS.gene68946 | 0.813131 | 2.99E-51 | -1.69E-46 |
| MS.gene052050 | MS.gene68946 | 0.800593 | 1.34E-48 | -1.69E-46 |
| MS.gene052129 | MS.gene68946 | 0.892603 | 1.36E-74 | -1.69E-46 |
| MS.gene052164 | MS.gene68946 | -0.834655 | 2.64E-56 | -1.69E-46 |
| MS.gene052379 | MS.gene68946 | -0.810929 | 9.03E-51 | -1.69E-46 |
| MS.gene05244 | MS.gene68946 | 0.811472 | 6.88E-51 | -1.69E-46 |
| MS.gene052503 | MS.gene68946 | -0.802441 | 5.60E-49 | -1.69E-46 |
| MS.gene052606 | MS.gene68946 | 0.815629 | 8.38E-52 | -1.69E-46 |
| MS.gene052615 | MS.gene68946 | -0.822682 | 2.08E-53 | -1.69E-46 |
| MS.gene052805 | MS.gene68946 | 0.809222 | 2.10E-50 | -1.69E-46 |
| MS.gene052825 | MS.gene68946 | -0.802592 | 5.22E-49 | -1.69E-46 |
| MS.gene052870 | MS.gene68946 | -0.844174 | 8.90E-59 | -1.69E-46 |
| MS.gene052872 | MS.gene68946 | -0.861661 | 8.77E-64 | -1.69E-46 |
| MS.gene052916 | MS.gene68946 | -0.806672 | 7.34E-50 | -1.69E-46 |
| MS.gene052966 | MS.gene68946 | -0.841174 | 5.58E-58 | -1.69E-46 |
| MS.gene053203 | MS.gene68946 | -0.847632 | 1.02E-59 | -1.69E-46 |
| MS.gene053284 | MS.gene68946 | -0.815995 | 6.95E-52 | -1.69E-46 |
| MS.gene053285 | MS.gene68946 | -0.806119 | 9.60E-50 | -1.69E-46 |
| MS.gene053420 | MS.gene68946 | 0.819288 | 1.26E-52 | -1.69E-46 |
| MS.gene053479 | MS.gene68946 | -0.841603 | 4.30E-58 | -1.69E-46 |
| MS.gene053488 | MS.gene68946 | 0.800646 | 1.31E-48 | -1.69E-46 |
| MS.gene053632 | MS.gene68946 | -0.838325 | 3.07E-57 | -1.69E-46 |
| MS.gene053776 | MS.gene68946 | 0.812277 | 4.60E-51 | -1.69E-46 |
| MS.gene053824 | MS.gene68946 | -0.843888 | 1.06E-58 | -1.69E-46 |
| MS.gene053916 | MS.gene68946 | -0.815123 | 1.09E-51 | -1.69E-46 |
| MS.gene054290 | MS.gene68946 | 0.812102 | 5.02E-51 | -1.69E-46 |
| MS.gene054344 | MS.gene68946 | -0.812353 | 4.42E-51 | -1.69E-46 |
| MS.gene054595 | MS.gene68946 | -0.818838 | 1.59E-52 | -1.69E-46 |
| MS.gene054600 | MS.gene68946 | -0.818785 | 1.64E-52 | -1.69E-46 |
| MS.gene054642 | MS.gene68946 | -0.835416 | 1.70E-56 | -1.69E-46 |
| MS.gene054706 | MS.gene68946 | 0.847435 | 1.16E-59 | -1.69E-46 |
| MS.gene05470 | MS.gene68946 | -0.856141 | 3.93E-62 | -1.69E-46 |
| MS.gene054884 | MS.gene68946 | 0.836358 | 9.81E-57 | -1.69E-46 |
| MS.gene05494 | MS.gene68946 | -0.817966 | 2.51E-52 | -1.69E-46 |
| MS.gene054954 | MS.gene68946 | -0.839815 | 1.26E-57 | -1.69E-46 |
| MS.gene055158 | MS.gene68946 | 0.814177 | 1.76E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene68946 | -0.837411 | 5.28E-57 | -1.69E-46 |
| MS.gene055251 | MS.gene68946 | 0.801267 | 9.76E-49 | -1.69E-46 |
| MS.gene055362 | MS.gene68946 | -0.834239 | 3.36E-56 | -1.69E-46 |
| MS.gene055502 | MS.gene68946 | -0.870171 | 1.78E-66 | -1.69E-46 |
| MS.gene055577 | MS.gene68946 | -0.872819 | 2.37E-67 | -1.69E-46 |
| MS.gene055748 | MS.gene68946 | -0.883903 | 3.02E-71 | -1.69E-46 |
| MS.gene055750 | MS.gene68946 | -0.849482 | 3.14E-60 | -1.69E-46 |
| MS.gene055751 | MS.gene68946 | -0.882487 | 1.00E-70 | -1.69E-46 |
| MS.gene055776 | MS.gene68946 | -0.804212 | 2.41E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene68946 | -0.864199 | 1.44E-64 | -1.69E-46 |
| MS.gene055941 | MS.gene68946 | -0.829344 | 5.42E-55 | -1.69E-46 |
| MS.gene056224 | MS.gene68946 | 0.809593 | 1.75E-50 | -1.69E-46 |
| MS.gene056283 | MS.gene68946 | -0.830506 | 2.83E-55 | -1.69E-46 |
| MS.gene056387 | MS.gene68946 | -0.833094 | 6.50E-56 | -1.69E-46 |
| MS.gene056504 | MS.gene68946 | -0.849018 | 4.23E-60 | -1.69E-46 |
| MS.gene056510 | MS.gene68946 | -0.854818 | 9.56E-62 | -1.69E-46 |
| MS.gene056618 | MS.gene68946 | 0.80276 | 4.82E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene68946 | -0.833561 | 4.97E-56 | -1.69E-46 |
| MS.gene056678 | MS.gene68946 | 0.847814 | 9.11E-60 | -1.69E-46 |
| MS.gene056688 | MS.gene68946 | 0.820618 | 6.24E-53 | -1.69E-46 |
| MS.gene056692 | MS.gene68946 | -0.83792 | 3.91E-57 | -1.69E-46 |
| MS.gene056697 | MS.gene68946 | 0.823089 | 1.67E-53 | -1.69E-46 |
| MS.gene056748 | MS.gene68946 | 0.805812 | 1.11E-49 | -1.69E-46 |
| MS.gene056856 | MS.gene68946 | 0.880975 | 3.52E-70 | -1.69E-46 |
| MS.gene056857 | MS.gene68946 | 0.808539 | 2.95E-50 | -1.69E-46 |
| MS.gene056903 | MS.gene68946 | -0.804392 | 2.21E-49 | -1.69E-46 |
| MS.gene057246 | MS.gene68946 | -0.810726 | 9.99E-51 | -1.69E-46 |
| MS.gene057332 | MS.gene68946 | -0.807699 | 4.45E-50 | -1.69E-46 |
| MS.gene05753 | MS.gene68946 | -0.808298 | 3.32E-50 | -1.69E-46 |
| MS.gene057544 | MS.gene68946 | 0.802513 | 5.41E-49 | -1.69E-46 |
| MS.gene057658 | MS.gene68946 | -0.827735 | 1.33E-54 | -1.69E-46 |
| MS.gene057717 | MS.gene68946 | 0.831403 | 1.70E-55 | -1.69E-46 |
| MS.gene057768 | MS.gene68946 | 0.808631 | 2.82E-50 | -1.69E-46 |
| MS.gene057831 | MS.gene68946 | -0.839814 | 1.26E-57 | -1.69E-46 |
| MS.gene057857 | MS.gene68946 | -0.819162 | 1.34E-52 | -1.69E-46 |
| MS.gene058158 | MS.gene68946 | 0.834316 | 3.22E-56 | -1.69E-46 |
| MS.gene058334 | MS.gene68946 | 0.859824 | 3.17E-63 | -1.69E-46 |
| MS.gene058338 | MS.gene68946 | -0.808712 | 2.71E-50 | -1.69E-46 |
| MS.gene05837 | MS.gene68946 | -0.833518 | 5.09E-56 | -1.69E-46 |
| MS.gene058382 | MS.gene68946 | 0.803344 | 3.65E-49 | -1.69E-46 |
| MS.gene058418 | MS.gene68946 | 0.817535 | 3.14E-52 | -1.69E-46 |
| MS.gene058419 | MS.gene68946 | -0.854851 | 9.34E-62 | -1.69E-46 |
| MS.gene058452 | MS.gene68946 | 0.807656 | 4.54E-50 | -1.69E-46 |
| MS.gene058544 | MS.gene68946 | -0.845431 | 4.08E-59 | -1.69E-46 |
| MS.gene058603 | MS.gene68946 | 0.84661 | 1.95E-59 | -1.69E-46 |
| MS.gene058723 | MS.gene68946 | -0.817541 | 3.13E-52 | -1.69E-46 |
| MS.gene058773 | MS.gene68946 | -0.839455 | 1.57E-57 | -1.69E-46 |
| MS.gene058838 | MS.gene68946 | -0.855733 | 5.17E-62 | -1.69E-46 |
| MS.gene059022 | MS.gene68946 | -0.850319 | 1.83E-60 | -1.69E-46 |
| MS.gene059023 | MS.gene68946 | -0.863698 | 2.07E-64 | -1.69E-46 |
| MS.gene059079 | MS.gene68946 | -0.816671 | 4.90E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene68946 | 0.8179 | 2.59E-52 | -1.69E-46 |
| MS.gene05914 | MS.gene68946 | -0.842066 | 3.24E-58 | -1.69E-46 |
| MS.gene05926 | MS.gene68946 | 0.84535 | 4.29E-59 | -1.69E-46 |
| MS.gene059304 | MS.gene68946 | 0.804416 | 2.18E-49 | -1.69E-46 |
| MS.gene05934 | MS.gene68946 | -0.804866 | 1.76E-49 | -1.69E-46 |
| MS.gene059526 | MS.gene68946 | 0.818072 | 2.37E-52 | -1.69E-46 |
| MS.gene05960 | MS.gene68946 | 0.823869 | 1.10E-53 | -1.69E-46 |
| MS.gene059667 | MS.gene68946 | -0.80428 | 2.33E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene68946 | -0.854373 | 1.29E-61 | -1.69E-46 |
| MS.gene059699 | MS.gene68946 | 0.851341 | 9.45E-61 | -1.69E-46 |
| MS.gene059701 | MS.gene68946 | 0.823012 | 1.74E-53 | -1.69E-46 |
| MS.gene059703 | MS.gene68946 | 0.852802 | 3.63E-61 | -1.69E-46 |
| MS.gene059718 | MS.gene68946 | -0.849329 | 3.47E-60 | -1.69E-46 |
| MS.gene059756 | MS.gene68946 | -0.819204 | 1.31E-52 | -1.69E-46 |
| MS.gene059801 | MS.gene68946 | -0.801231 | 9.93E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene68946 | -0.849517 | 3.07E-60 | -1.69E-46 |
| MS.gene059955 | MS.gene68946 | 0.804022 | 2.64E-49 | -1.69E-46 |
| MS.gene06000 | MS.gene68946 | -0.836397 | 9.58E-57 | -1.69E-46 |
| MS.gene060405 | MS.gene68946 | 0.848881 | 4.62E-60 | -1.69E-46 |
| MS.gene060703 | MS.gene68946 | -0.806094 | 9.72E-50 | -1.69E-46 |
| MS.gene060762 | MS.gene68946 | -0.874925 | 4.61E-68 | -1.69E-46 |
| MS.gene060778 | MS.gene68946 | -0.813555 | 2.41E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene68946 | -0.827851 | 1.25E-54 | -1.69E-46 |
| MS.gene060823 | MS.gene68946 | 0.829647 | 4.58E-55 | -1.69E-46 |
| MS.gene06092 | MS.gene68946 | -0.810949 | 8.94E-51 | -1.69E-46 |
| MS.gene060982 | MS.gene68946 | -0.863922 | 1.76E-64 | -1.69E-46 |
| MS.gene061044 | MS.gene68946 | 0.852547 | 4.30E-61 | -1.69E-46 |
| MS.gene061150 | MS.gene68946 | -0.853715 | 1.99E-61 | -1.69E-46 |
| MS.gene061231 | MS.gene68946 | -0.810678 | 1.02E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68946.t1 | MTR_4g132400 | 92.508 | 307 | 17 | 1 | 1 | 307 | 1 | 301 | 0.0 | 571 |
| MS.gene68946.t1 | MTR_4g049640 | 50.709 | 282 | 138 | 1 | 1 | 282 | 1 | 281 | 3.93e-84 | 258 |
| MS.gene68946.t1 | MTR_3g112530 | 52.941 | 238 | 110 | 2 | 45 | 281 | 43 | 279 | 1.28e-77 | 242 |
| MS.gene68946.t1 | MTR_4g101880 | 39.844 | 256 | 146 | 4 | 39 | 293 | 177 | 425 | 3.37e-54 | 186 |
| MS.gene68946.t1 | MTR_6g034030 | 37.838 | 259 | 153 | 4 | 38 | 296 | 227 | 477 | 2.63e-53 | 182 |
| MS.gene68946.t1 | MTR_6g034030 | 37.984 | 258 | 152 | 4 | 38 | 295 | 227 | 476 | 1.46e-52 | 182 |
| MS.gene68946.t1 | MTR_0549s0020 | 36.364 | 275 | 166 | 6 | 25 | 299 | 143 | 408 | 1.82e-51 | 178 |
| MS.gene68946.t1 | MTR_6g045200 | 37.209 | 258 | 154 | 5 | 38 | 295 | 218 | 467 | 2.21e-50 | 176 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68946.t1 | AT1G62280 | 50.825 | 303 | 130 | 5 | 26 | 320 | 11 | 302 | 6.80e-91 | 276 |
| MS.gene68946.t1 | AT1G62262 | 49.186 | 307 | 126 | 5 | 19 | 320 | 13 | 294 | 2.11e-87 | 267 |
| MS.gene68946.t1 | AT5G24030 | 39.130 | 253 | 146 | 3 | 43 | 295 | 253 | 497 | 1.78e-53 | 183 |
| MS.gene68946.t1 | AT5G24030 | 39.130 | 253 | 146 | 3 | 43 | 295 | 253 | 497 | 1.50e-52 | 183 |
| MS.gene68946.t1 | AT4G27970 | 37.008 | 254 | 146 | 4 | 43 | 293 | 138 | 380 | 1.10e-48 | 167 |
| MS.gene68946.t1 | AT4G27970 | 37.008 | 254 | 146 | 4 | 43 | 293 | 138 | 380 | 1.10e-48 | 167 |
| MS.gene68946.t1 | AT1G12480 | 36.803 | 269 | 162 | 3 | 26 | 293 | 167 | 428 | 1.60e-48 | 171 |
| MS.gene68946.t1 | AT4G27970 | 37.008 | 254 | 146 | 4 | 43 | 293 | 138 | 380 | 1.42e-47 | 167 |
| MS.gene68946.t1 | AT4G27970 | 37.008 | 254 | 146 | 4 | 43 | 293 | 138 | 380 | 1.42e-47 | 167 |
| MS.gene68946.t1 | AT4G27970 | 37.603 | 242 | 137 | 4 | 55 | 293 | 1 | 231 | 6.58e-47 | 162 |
| MS.gene68946.t1 | AT4G27970 | 37.603 | 242 | 137 | 4 | 55 | 293 | 1 | 231 | 6.58e-47 | 162 |
Find 53 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCAACCTGGTTTCTGTTAT+AGG | 0.207016 | 8.2:+1703408 | MS.gene68946:CDS |
| GCTAAGCCAAGGAATGTTAA+AGG | 0.296789 | 8.2:-1703862 | None:intergenic |
| TCAACCTGGTTTCTGTTATA+GGG | 0.297612 | 8.2:+1703409 | MS.gene68946:CDS |
| GACCTACCTGAGAAATAAAA+AGG | 0.303132 | 8.2:-1703692 | None:intergenic |
| GAATGTGATGGCTTGGTGAT+TGG | 0.310351 | 8.2:-1702919 | None:intergenic |
| GCAATTTCAATGTATTCATT+AGG | 0.313578 | 8.2:+1703480 | MS.gene68946:CDS |
| CTGTTGCTTATCTATTACTT+TGG | 0.340533 | 8.2:+1703088 | MS.gene68946:CDS |
| GATGAATTTGCACATCATAT+TGG | 0.345252 | 8.2:+1703189 | MS.gene68946:CDS |
| TCATTCCCTTTAACATTCCT+TGG | 0.356219 | 8.2:+1703856 | MS.gene68946:CDS |
| CAAGCTAGACTTGCCATACT+TGG | 0.362804 | 8.2:-1703609 | None:intergenic |
| CACACAATTATGTCAATTGT+TGG | 0.378332 | 8.2:-1702847 | None:intergenic |
| ACAATGAATCACAAACTCTT+TGG | 0.388818 | 8.2:+1703043 | MS.gene68946:CDS |
| AATGTGATGGCTTGGTGATT+GGG | 0.406822 | 8.2:-1702918 | None:intergenic |
| TGCTTCAAGCATCACCGCCT+TGG | 0.430111 | 8.2:+1703253 | MS.gene68946:CDS |
| GCTCAAGTTATGACAGAAAT+CGG | 0.453671 | 8.2:+1703444 | MS.gene68946:CDS |
| ATAACAGAAACCAGGTTGAC+AGG | 0.456215 | 8.2:-1703405 | None:intergenic |
| GGTTTCTGTTATAGGGAACT+TGG | 0.463993 | 8.2:+1703416 | MS.gene68946:CDS |
| TTGGTGCTCAAGCTTTGCTA+TGG | 0.471950 | 8.2:+1703001 | MS.gene68946:CDS |
| TTAACTACATGTATACTCCT+TGG | 0.476184 | 8.2:+1703214 | MS.gene68946:CDS |
| CTAAGCCAAGGAATGTTAAA+GGG | 0.477768 | 8.2:-1703861 | None:intergenic |
| TGACTCTGAATGTGATGGCT+TGG | 0.485943 | 8.2:-1702926 | None:intergenic |
| GAGCATATAAAGAAAGGATA+AGG | 0.489737 | 8.2:-1703136 | None:intergenic |
| GCATGGATCTTAGTTAAGAA+TGG | 0.489812 | 8.2:-1702952 | None:intergenic |
| TTCAATGTATTCATTAGGAA+TGG | 0.492135 | 8.2:+1703485 | MS.gene68946:CDS |
| CAAGTATGGCAAGTCTAGCT+TGG | 0.498344 | 8.2:+1703610 | MS.gene68946:CDS |
| CTAGCTTGGAAATCCATCTC+AGG | 0.499285 | 8.2:+1703624 | MS.gene68946:CDS |
| GCACTTGAGCATATAAAGAA+AGG | 0.500953 | 8.2:-1703142 | None:intergenic |
| TGTTCAAGAAAACTATGAAA+AGG | 0.514872 | 8.2:+1703809 | MS.gene68946:CDS |
| AGCACTAGTAGTATTGTTCG+TGG | 0.515674 | 8.2:-1702875 | None:intergenic |
| GTGATGGCTTGGTGATTGGG+TGG | 0.520148 | 8.2:-1702915 | None:intergenic |
| AGTAGCAAACCCTGTCAACC+TGG | 0.530614 | 8.2:+1703395 | MS.gene68946:CDS |
| TTCTGTTATAGGGAACTTGG+TGG | 0.536173 | 8.2:+1703419 | MS.gene68946:CDS |
| GAATCACAAACTCTTTGGCA+TGG | 0.539825 | 8.2:+1703048 | MS.gene68946:CDS |
| AATGGTTCACTACTGAAAAG+AGG | 0.541292 | 8.2:+1703361 | MS.gene68946:CDS |
| TAAGCTGGTCTTAGCACTAC+AGG | 0.541582 | 8.2:-1703567 | None:intergenic |
| ACTATAGAATAGAATGGGTG+AGG | 0.549206 | 8.2:+1702813 | None:intergenic |
| GACGAGAAAAGCACCTGAGA+TGG | 0.550784 | 8.2:-1703637 | None:intergenic |
| AAGTTATGACAGAAATCGGT+TGG | 0.554540 | 8.2:+1703448 | MS.gene68946:CDS |
| TGATGGCTTGGTGATTGGGT+GGG | 0.556380 | 8.2:-1702914 | None:intergenic |
| CTTGCACTTTAATGCAGTGA+AGG | 0.559661 | 8.2:+1703167 | MS.gene68946:CDS |
| TAGCAAAGCTTGAGCACCAA+AGG | 0.566448 | 8.2:-1702998 | None:intergenic |
| TTCTTAACTAAGATCCATGC+AGG | 0.573358 | 8.2:+1702955 | MS.gene68946:CDS |
| TGAAAAGGCTAAACGTTACA+TGG | 0.577666 | 8.2:+1703824 | MS.gene68946:CDS |
| TAACAGAAACCAGGTTGACA+GGG | 0.582523 | 8.2:-1703404 | None:intergenic |
| AGTAATAGATAAGCAACAGA+AGG | 0.591520 | 8.2:-1703084 | None:intergenic |
| ATGAGAAGATATGAAATCCA+AGG | 0.593130 | 8.2:-1703231 | None:intergenic |
| CATATAAAGAAAGGATAAGG+TGG | 0.598970 | 8.2:-1703133 | None:intergenic |
| GGTTGTGACTCTGAATGTGA+TGG | 0.605496 | 8.2:-1702931 | None:intergenic |
| ATTCAGCACAAGCTAAGCCA+AGG | 0.637854 | 8.2:-1703873 | None:intergenic |
| CAAGTTCTCGAAACAATCCA+AGG | 0.648724 | 8.2:-1703270 | None:intergenic |
| AAAGGCTAAACGTTACATGG+TGG | 0.692493 | 8.2:+1703827 | MS.gene68946:CDS |
| AGTTCCCTATAACAGAAACC+AGG | 0.725065 | 8.2:-1703413 | None:intergenic |
| GTTCTCGAAACAATCCAAGG+CGG | 0.791324 | 8.2:-1703267 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAGCAACTAAATATATAT+AGG | - | chr8.2:1703727-1703746 | None:intergenic | 15.0% |
| !! | TAAGCAACTAAATATATATA+GGG | - | chr8.2:1703726-1703745 | None:intergenic | 15.0% |
| !!! | AAATATATATAGGGTAATTT+TGG | - | chr8.2:1703717-1703736 | None:intergenic | 15.0% |
| ! | GCAATTTCAATGTATTCATT+AGG | + | chr8.2:1703480-1703499 | MS.gene68946:CDS | 25.0% |
| ! | TGTTCAAGAAAACTATGAAA+AGG | + | chr8.2:1703809-1703828 | MS.gene68946:CDS | 25.0% |
| ! | TTCAATGTATTCATTAGGAA+TGG | + | chr8.2:1703485-1703504 | MS.gene68946:CDS | 25.0% |
| !! | ATGTAAAACTTTTTGGACAA+TGG | + | chr8.2:1703343-1703362 | MS.gene68946:CDS | 25.0% |
| !! | TTTTTCATTTGTGTTTCCTT+TGG | + | chr8.2:1702982-1703001 | MS.gene68946:CDS | 25.0% |
| !!! | TTTGTTCCTTTTTATTTCTC+AGG | + | chr8.2:1703686-1703705 | MS.gene68946:CDS | 25.0% |
| ACAATGAATCACAAACTCTT+TGG | + | chr8.2:1703043-1703062 | MS.gene68946:CDS | 30.0% | |
| AGTAATAGATAAGCAACAGA+AGG | - | chr8.2:1703087-1703106 | None:intergenic | 30.0% | |
| ATGAGAAGATATGAAATCCA+AGG | - | chr8.2:1703234-1703253 | None:intergenic | 30.0% | |
| CACACAATTATGTCAATTGT+TGG | - | chr8.2:1702850-1702869 | None:intergenic | 30.0% | |
| CATATAAAGAAAGGATAAGG+TGG | - | chr8.2:1703136-1703155 | None:intergenic | 30.0% | |
| CCATTTCATTGTAATGATCA+AGG | + | chr8.2:1703756-1703775 | MS.gene68946:intron | 30.0% | |
| CCTTGATCATTACAATGAAA+TGG | - | chr8.2:1703759-1703778 | None:intergenic | 30.0% | |
| GAGCATATAAAGAAAGGATA+AGG | - | chr8.2:1703139-1703158 | None:intergenic | 30.0% | |
| GATGAATTTGCACATCATAT+TGG | + | chr8.2:1703189-1703208 | MS.gene68946:CDS | 30.0% | |
| GCAAAAAACAAGAAATAAGC+TGG | - | chr8.2:1703585-1703604 | None:intergenic | 30.0% | |
| TTAACTACATGTATACTCCT+TGG | + | chr8.2:1703214-1703233 | MS.gene68946:CDS | 30.0% | |
| ! | CTGCTTGATGTAAAACTTTT+TGG | + | chr8.2:1703336-1703355 | MS.gene68946:CDS | 30.0% |
| ! | CTGTTGCTTATCTATTACTT+TGG | + | chr8.2:1703088-1703107 | MS.gene68946:CDS | 30.0% |
| !! | TTCCTTTTTATTTCTCAGGT+AGG | + | chr8.2:1703690-1703709 | MS.gene68946:intron | 30.0% |
| AAATGAAAAAGTAGCCTGCA+TGG | - | chr8.2:1702972-1702991 | None:intergenic | 35.0% | |
| AAGTTATGACAGAAATCGGT+TGG | + | chr8.2:1703448-1703467 | MS.gene68946:CDS | 35.0% | |
| AATGGTTCACTACTGAAAAG+AGG | + | chr8.2:1703361-1703380 | MS.gene68946:CDS | 35.0% | |
| CTAAGCCAAGGAATGTTAAA+GGG | - | chr8.2:1703864-1703883 | None:intergenic | 35.0% | |
| GACCTACCTGAGAAATAAAA+AGG | - | chr8.2:1703695-1703714 | None:intergenic | 35.0% | |
| GATCAAGGCTTATATAATGC+AGG | + | chr8.2:1703771-1703790 | MS.gene68946:intron | 35.0% | |
| GCACTTGAGCATATAAAGAA+AGG | - | chr8.2:1703145-1703164 | None:intergenic | 35.0% | |
| GCATGGATCTTAGTTAAGAA+TGG | - | chr8.2:1702955-1702974 | None:intergenic | 35.0% | |
| GCTCAAGTTATGACAGAAAT+CGG | + | chr8.2:1703444-1703463 | MS.gene68946:CDS | 35.0% | |
| TCAACCTGGTTTCTGTTATA+GGG | + | chr8.2:1703409-1703428 | MS.gene68946:CDS | 35.0% | |
| TCATTCCCTTTAACATTCCT+TGG | + | chr8.2:1703856-1703875 | MS.gene68946:CDS | 35.0% | |
| TGAAAAGGCTAAACGTTACA+TGG | + | chr8.2:1703824-1703843 | MS.gene68946:CDS | 35.0% | |
| TTCTTAACTAAGATCCATGC+AGG | + | chr8.2:1702955-1702974 | MS.gene68946:CDS | 35.0% | |
| ! | ATAGTTTTCTTGAACAGTGC+TGG | - | chr8.2:1703805-1703824 | None:intergenic | 35.0% |
| AAAGGCTAAACGTTACATGG+TGG | + | chr8.2:1703827-1703846 | MS.gene68946:CDS | 40.0% | |
| AGCACTAGTAGTATTGTTCG+TGG | - | chr8.2:1702878-1702897 | None:intergenic | 40.0% | |
| AGTTCCCTATAACAGAAACC+AGG | - | chr8.2:1703416-1703435 | None:intergenic | 40.0% | |
| CAAGTTCTCGAAACAATCCA+AGG | - | chr8.2:1703273-1703292 | None:intergenic | 40.0% | |
| CTTATATAATGCAGGCTTGC+AGG | + | chr8.2:1703779-1703798 | MS.gene68946:intron | 40.0% | |
| CTTGCACTTTAATGCAGTGA+AGG | + | chr8.2:1703167-1703186 | MS.gene68946:CDS | 40.0% | |
| GAATCACAAACTCTTTGGCA+TGG | + | chr8.2:1703048-1703067 | MS.gene68946:CDS | 40.0% | |
| GCTAAGCCAAGGAATGTTAA+AGG | - | chr8.2:1703865-1703884 | None:intergenic | 40.0% | |
| GGTTTCTGTTATAGGGAACT+TGG | + | chr8.2:1703416-1703435 | MS.gene68946:CDS | 40.0% | |
| GTCAACCTGGTTTCTGTTAT+AGG | + | chr8.2:1703408-1703427 | MS.gene68946:CDS | 40.0% | |
| TTCTGTTATAGGGAACTTGG+TGG | + | chr8.2:1703419-1703438 | MS.gene68946:CDS | 40.0% | |
| ! | ATAACAGAAACCAGGTTGAC+AGG | - | chr8.2:1703408-1703427 | None:intergenic | 40.0% |
| ! | TAACAGAAACCAGGTTGACA+GGG | - | chr8.2:1703407-1703426 | None:intergenic | 40.0% |
| !! | AATGTGATGGCTTGGTGATT+GGG | - | chr8.2:1702921-1702940 | None:intergenic | 40.0% |
| !!! | GTTTTTTGCTGCACCAAGTA+TGG | + | chr8.2:1703596-1703615 | MS.gene68946:CDS | 40.0% |
| CAAGCTAGACTTGCCATACT+TGG | - | chr8.2:1703612-1703631 | None:intergenic | 45.0% | |
| CAAGTATGGCAAGTCTAGCT+TGG | + | chr8.2:1703610-1703629 | MS.gene68946:CDS | 45.0% | |
| CTAGCTTGGAAATCCATCTC+AGG | + | chr8.2:1703624-1703643 | MS.gene68946:CDS | 45.0% | |
| GGTTGTGACTCTGAATGTGA+TGG | - | chr8.2:1702934-1702953 | None:intergenic | 45.0% | |
| GTTCTCGAAACAATCCAAGG+CGG | - | chr8.2:1703270-1703289 | None:intergenic | 45.0% | |
| TAGCAAAGCTTGAGCACCAA+AGG | - | chr8.2:1703001-1703020 | None:intergenic | 45.0% | |
| TGACTCTGAATGTGATGGCT+TGG | - | chr8.2:1702929-1702948 | None:intergenic | 45.0% | |
| ! | TTGGTGCTCAAGCTTTGCTA+TGG | + | chr8.2:1703001-1703020 | MS.gene68946:CDS | 45.0% |
| !! | GAATGTGATGGCTTGGTGAT+TGG | - | chr8.2:1702922-1702941 | None:intergenic | 45.0% |
| !! | TAAGCTGGTCTTAGCACTAC+AGG | - | chr8.2:1703570-1703589 | None:intergenic | 45.0% |
| AGTAGCAAACCCTGTCAACC+TGG | + | chr8.2:1703395-1703414 | MS.gene68946:CDS | 50.0% | |
| !! | GACGAGAAAAGCACCTGAGA+TGG | - | chr8.2:1703640-1703659 | None:intergenic | 50.0% |
| !! | TGATGGCTTGGTGATTGGGT+GGG | - | chr8.2:1702917-1702936 | None:intergenic | 50.0% |
| TGCTTCAAGCATCACCGCCT+TGG | + | chr8.2:1703253-1703272 | MS.gene68946:CDS | 55.0% | |
| !! | GTGATGGCTTGGTGATTGGG+TGG | - | chr8.2:1702918-1702937 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 1702826 | 1703893 | 1702826 | ID=MS.gene68946 |
| chr8.2 | mRNA | 1702826 | 1703893 | 1702826 | ID=MS.gene68946.t1;Parent=MS.gene68946 |
| chr8.2 | exon | 1702826 | 1703707 | 1702826 | ID=MS.gene68946.t1.exon1;Parent=MS.gene68946.t1 |
| chr8.2 | CDS | 1702826 | 1703707 | 1702826 | ID=cds.MS.gene68946.t1;Parent=MS.gene68946.t1 |
| chr8.2 | exon | 1703801 | 1703893 | 1703801 | ID=MS.gene68946.t1.exon2;Parent=MS.gene68946.t1 |
| chr8.2 | CDS | 1703801 | 1703893 | 1703801 | ID=cds.MS.gene68946.t1;Parent=MS.gene68946.t1 |
| Gene Sequence |
| Protein sequence |
