AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069871


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069871.t1 MTR_2g101130 96.063 127 2 1 1 127 34 157 6.54e-86 249
MS.gene069871.t1 MTR_2g101120 85.039 127 11 3 1 127 34 152 5.21e-70 209
MS.gene069871.t1 MTR_8g014030 63.281 128 33 5 1 127 34 148 1.73e-49 156
MS.gene069871.t1 MTR_8g014010 62.791 129 35 6 1 127 34 151 7.08e-46 147
MS.gene069871.t1 MTR_8g014020 60.748 107 28 5 22 127 1 94 2.95e-37 124
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069871.t1 AT1G52140 43.802 121 54 4 21 131 45 161 7.71e-23 89.4
MS.gene069871.t1 AT3G16330 44.444 90 33 3 51 126 65 151 3.49e-16 71.6

Find 37 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCAGCACTGCCTGGATT+TGG 0.243350 2.1:+3876123 MS.gene069871:CDS
ACTCCCTTGATGCGGTAAAT+TGG 0.283815 2.1:-3875906 None:intergenic
CTCCAGCACTGCCTGGATTT+GGG 0.291256 2.1:+3876124 MS.gene069871:CDS
TGCGGTGACGTTTCCCTATT+GGG 0.299531 2.1:-3875966 None:intergenic
CTGCGGTGACGTTTCCCTAT+TGG 0.308179 2.1:-3875967 None:intergenic
CGCCCAAATCCAGGCAGTGC+TGG 0.418682 2.1:-3876126 None:intergenic
GGCGTTTGAGCATCATGTTT+AGG 0.451131 2.1:-3875780 None:intergenic
CGTGGCTGTGGTGGCTGTGA+TGG 0.466817 2.1:-3875846 None:intergenic
TGAAGAAATTGTTGTGATTG+TGG 0.472141 2.1:-3875996 None:intergenic
AGGTGGAGCCGCCGTGGCTG+TGG 0.473691 2.1:-3875858 None:intergenic
CCCTTGATGCGGTAAATTGG+TGG 0.485419 2.1:-3875903 None:intergenic
GAAGCATCTCCAGCACTGCC+TGG 0.487263 2.1:+3876117 MS.gene069871:CDS
GAGTGCAATGAAGGCCGTGC+TGG 0.499574 2.1:+3876065 MS.gene069871:CDS
CATCACAGCCACCACAGCCA+CGG 0.499967 2.1:+3875847 MS.gene069871:CDS
GTTGGGCTACGCCCAAATCC+AGG 0.505331 2.1:-3876135 None:intergenic
GTCGTGAGAGCGGCTTGAGG+TGG 0.508313 2.1:-3875875 None:intergenic
TTACTGATTGATTAAGGCAA+AGG 0.523427 2.1:-3877301 None:intergenic
GGCTGTGGTGGCTGTGATGG+TGG 0.525762 2.1:-3875843 None:intergenic
GCCACCAATTTACCGCATCA+AGG 0.534674 2.1:+3875902 MS.gene069871:CDS
TGGAGCCGCCGTGGCTGTGG+TGG 0.543982 2.1:-3875855 None:intergenic
CGCTCATACGCCACCTACAC+AGG 0.551605 2.1:+3876023 MS.gene069871:CDS
AAACGCCGAAGCAAGCTCGC+CGG 0.566114 2.1:+3875796 MS.gene069871:CDS
GGCGACAATGAGTGCAATGA+AGG 0.584967 2.1:+3876056 MS.gene069871:CDS
ACATCATCGTCCTGTGTAGG+TGG 0.587034 2.1:-3876033 None:intergenic
CCACCAATTTACCGCATCAA+GGG 0.590913 2.1:+3875903 MS.gene069871:CDS
GTGGAACATTAGAATGGCAA+TGG 0.596299 2.1:-3875824 None:intergenic
TGGATTTGGGCGTAGCCCAA+CGG 0.614372 2.1:+3876137 MS.gene069871:CDS
GGCTTGAGGTGGAGCCGCCG+TGG 0.620681 2.1:-3875864 None:intergenic
GTGATGGTGGAACATTAGAA+TGG 0.624559 2.1:-3875830 None:intergenic
GGTGGCGTGAGTCGTGAGAG+CGG 0.626926 2.1:-3875885 None:intergenic
TGTGATTGTGGTTGTTGCTG+CGG 0.635520 2.1:-3875984 None:intergenic
GCCACATCATCGTCCTGTGT+AGG 0.639849 2.1:-3876036 None:intergenic
ATTGTTAAGCATCTCCAGCA+CGG 0.641871 2.1:-3876079 None:intergenic
CACAGCCACCACAGCCACGG+CGG 0.652559 2.1:+3875850 MS.gene069871:CDS
ACCTACACAGGACGATGATG+TGG 0.656484 2.1:+3876035 MS.gene069871:CDS
TGAGTCGTGAGAGCGGCTTG+AGG 0.683296 2.1:-3875878 None:intergenic
GAATTCATACTCCCTTGATG+CGG 0.696823 2.1:-3875914 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTGATTATTATTAAGAA+AGG - chr2.1:3876796-3876815 None:intergenic 15.0%
!! CATTAATTATTAACTAAACA+AGG - chr2.1:3876320-3876339 None:intergenic 15.0%
!! TTTAGTTAATAATTAATGTG+TGG + chr2.1:3876322-3876341 MS.gene069871:intron 15.0%
!!! AAAAATTTCAATGAATCTTT+TGG + chr2.1:3877149-3877168 MS.gene069871:intron 15.0%
!!! GTTTTTTCTCAAATAAATTA+CGG + chr2.1:3876576-3876595 MS.gene069871:intron 15.0%
!!! TATATATGAGATTCTTTTTA+AGG - chr2.1:3876648-3876667 None:intergenic 15.0%
!!! TTTTGTTAGAAATTATGAAA+AGG - chr2.1:3876611-3876630 None:intergenic 15.0%
!! AAACTTGAAATATGTGTTAA+GGG + chr2.1:3876943-3876962 MS.gene069871:intron 20.0%
!! AAATAACCAAAAATGCAAAA+TGG - chr2.1:3876374-3876393 None:intergenic 20.0%
!! AAGAATCTCATATATATGTT+TGG + chr2.1:3876652-3876671 MS.gene069871:intron 20.0%
!! ATAAGAGAATAAATTACAGA+TGG + chr2.1:3876519-3876538 MS.gene069871:intron 20.0%
!! TAAACTTGAAATATGTGTTA+AGG + chr2.1:3876942-3876961 MS.gene069871:intron 20.0%
!!! TCAACAATTTTTTTTGAACA+AGG - chr2.1:3876484-3876503 None:intergenic 20.0%
! AACTTTACGAATTAATTAGC+CGG + chr2.1:3877059-3877078 MS.gene069871:intron 25.0%
! TGTTAGAGTACATAATATAC+CGG - chr2.1:3877081-3877100 None:intergenic 25.0%
!! AAGTAACCATTTTGCATTTT+TGG + chr2.1:3876365-3876384 MS.gene069871:intron 25.0%
!! TCAATGAATCTTTTGGATTT+AGG + chr2.1:3877156-3877175 MS.gene069871:intron 25.0%
ATTAACTAAACAAGGTACGT+AGG - chr2.1:3876312-3876331 None:intergenic 30.0%
TGAAGAAATTGTTGTGATTG+TGG - chr2.1:3875999-3876018 None:intergenic 30.0%
! TTGGATTTAGGATATACTCT+TGG + chr2.1:3877168-3877187 MS.gene069871:intron 30.0%
!! TATGATCCAGAGTTGATATA+CGG + chr2.1:3876402-3876421 MS.gene069871:intron 30.0%
AACATGATCGAATCTTTCTG+AGG + chr2.1:3877101-3877120 MS.gene069871:intron 35.0%
AGTACAAAGATGTGTACGAA+GGG - chr2.1:3877129-3877148 None:intergenic 35.0%
ATCAATCTTGCTTCCTCAAT+TGG - chr2.1:3876269-3876288 None:intergenic 35.0%
CTGCTGAAGATTTCATAAAC+AGG + chr2.1:3876235-3876254 MS.gene069871:intron 35.0%
TACCAATTGAGTTAAGCTCA+CGG - chr2.1:3876692-3876711 None:intergenic 35.0%
TAGTACAAAGATGTGTACGA+AGG - chr2.1:3877130-3877149 None:intergenic 35.0%
TCTAAGAGAAGATGATGAGA+AGG + chr2.1:3876194-3876213 MS.gene069871:intron 35.0%
TCTCATCATCTTCTCTTAGA+GGG - chr2.1:3876194-3876213 None:intergenic 35.0%
TTACAACCCAAAATTCTGCA+AGG - chr2.1:3877208-3877227 None:intergenic 35.0%
TTCTCATCATCTTCTCTTAG+AGG - chr2.1:3876195-3876214 None:intergenic 35.0%
! ATAAGCACCTTGCAGAATTT+TGG + chr2.1:3877198-3877217 MS.gene069871:intron 35.0%
! GACGGTGATTTTCTTCTTTT+TGG - chr2.1:3876749-3876768 None:intergenic 35.0%
! GGTGATTTTCTTCTTTTTGG+TGG - chr2.1:3876746-3876765 None:intergenic 35.0%
! TAAGCACCTTGCAGAATTTT+GGG + chr2.1:3877199-3877218 MS.gene069871:intron 35.0%
ACTTTACGTACTCTCGCATT+TGG + chr2.1:3876857-3876876 MS.gene069871:intron 40.0%
ATTGTTAAGCATCTCCAGCA+CGG - chr2.1:3876082-3876101 None:intergenic 40.0%
CCCTATTGGGAAAAAGTGAT+GGG - chr2.1:3875956-3875975 None:intergenic 40.0%
CCTATTGGGAAAAAGTGATG+GGG - chr2.1:3875955-3875974 None:intergenic 40.0%
CGTATAAATCTCCCACATGT+AGG - chr2.1:3876430-3876449 None:intergenic 40.0%
GAATTCATACTCCCTTGATG+CGG - chr2.1:3875917-3875936 None:intergenic 40.0%
GAGAAAAAACACAAGAGCAG+CGG - chr2.1:3876566-3876585 None:intergenic 40.0%
GATATACGGTACCTACATGT+GGG + chr2.1:3876416-3876435 MS.gene069871:intron 40.0%
GTGGAACATTAGAATGGCAA+TGG - chr2.1:3875827-3875846 None:intergenic 40.0%
TCATCTTCTCTTAGAGGGAA+TGG - chr2.1:3876189-3876208 None:intergenic 40.0%
TCCCTATTGGGAAAAAGTGA+TGG - chr2.1:3875957-3875976 None:intergenic 40.0%
TGATATACGGTACCTACATG+TGG + chr2.1:3876415-3876434 MS.gene069871:intron 40.0%
! ACAGGTTTTACAGCCAATTG+AGG + chr2.1:3876253-3876272 MS.gene069871:intron 40.0%
! TAGAATGGCAATGGCTTTTC+CGG - chr2.1:3875818-3875837 None:intergenic 40.0%
! TAGGTACCGTATATCAACTC+TGG - chr2.1:3876411-3876430 None:intergenic 40.0%
!! GAAGGACGATCAAGTTGATA+AGG + chr2.1:3876212-3876231 MS.gene069871:intron 40.0%
!! GTGATGGTGGAACATTAGAA+TGG - chr2.1:3875833-3875852 None:intergenic 40.0%
!! TGTTTTTGTGAGCACGATGA+CGG - chr2.1:3876767-3876786 None:intergenic 40.0%
!!! CCCATCACTTTTTCCCAATA+GGG + chr2.1:3875953-3875972 MS.gene069871:CDS 40.0%
AAAAAACACAAGAGCAGCGG+TGG - chr2.1:3876563-3876582 None:intergenic 45.0%
ACTCCCTTGATGCGGTAAAT+TGG - chr2.1:3875909-3875928 None:intergenic 45.0%
CACCGTGAGCTTAACTCAAT+TGG + chr2.1:3876687-3876706 MS.gene069871:intron 45.0%
CCACCAATTTACCGCATCAA+GGG + chr2.1:3875903-3875922 MS.gene069871:CDS 45.0%
GGCGTTTGAGCATCATGTTT+AGG - chr2.1:3875783-3875802 None:intergenic 45.0%
TGTGATTGTGGTTGTTGCTG+CGG - chr2.1:3875987-3876006 None:intergenic 45.0%
!!! CCCCATCACTTTTTCCCAAT+AGG + chr2.1:3875952-3875971 MS.gene069871:CDS 45.0%
!!! TTTTAAATTCTAAATTTTTT+TGG + chr2.1:3876813-3876832 MS.gene069871:intron 5.0%
ACATCATCGTCCTGTGTAGG+TGG - chr2.1:3876036-3876055 None:intergenic 50.0%
ACCCTCAATTGCCTTACCGT+TGG - chr2.1:3876156-3876175 None:intergenic 50.0%
ACCTACACAGGACGATGATG+TGG + chr2.1:3876035-3876054 MS.gene069871:CDS 50.0%
CCCAACGGTAAGGCAATTGA+GGG + chr2.1:3876152-3876171 MS.gene069871:intron 50.0%
CCCTCAATTGCCTTACCGTT+GGG - chr2.1:3876155-3876174 None:intergenic 50.0%
CCCTTGATGCGGTAAATTGG+TGG - chr2.1:3875906-3875925 None:intergenic 50.0%
GCCACCAATTTACCGCATCA+AGG + chr2.1:3875902-3875921 MS.gene069871:CDS 50.0%
GGCGACAATGAGTGCAATGA+AGG + chr2.1:3876056-3876075 MS.gene069871:CDS 50.0%
TGCGGTGACGTTTCCCTATT+GGG - chr2.1:3875969-3875988 None:intergenic 50.0%
CTCCAGCACTGCCTGGATTT+GGG + chr2.1:3876124-3876143 MS.gene069871:CDS 55.0%
CTGCGGTGACGTTTCCCTAT+TGG - chr2.1:3875970-3875989 None:intergenic 55.0%
GCCACATCATCGTCCTGTGT+AGG - chr2.1:3876039-3876058 None:intergenic 55.0%
GCCCAACGGTAAGGCAATTG+AGG + chr2.1:3876151-3876170 MS.gene069871:intron 55.0%
TCTCCAGCACTGCCTGGATT+TGG + chr2.1:3876123-3876142 MS.gene069871:CDS 55.0%
TGGATTTGGGCGTAGCCCAA+CGG + chr2.1:3876137-3876156 MS.gene069871:CDS 55.0%
!! CTTTTCCGGCGAGCTTGCTT+CGG - chr2.1:3875804-3875823 None:intergenic 55.0%
AAACGCCGAAGCAAGCTCGC+CGG + chr2.1:3875796-3875815 MS.gene069871:CDS 60.0%
CATCACAGCCACCACAGCCA+CGG + chr2.1:3875847-3875866 MS.gene069871:CDS 60.0%
CGCTCATACGCCACCTACAC+AGG + chr2.1:3876023-3876042 MS.gene069871:CDS 60.0%
GAGTGCAATGAAGGCCGTGC+TGG + chr2.1:3876065-3876084 MS.gene069871:CDS 60.0%
GTTGGGCTACGCCCAAATCC+AGG - chr2.1:3876138-3876157 None:intergenic 60.0%
TGAGTCGTGAGAGCGGCTTG+AGG - chr2.1:3875881-3875900 None:intergenic 60.0%
TTGGGCGTAGCCCAACGGTA+AGG + chr2.1:3876142-3876161 MS.gene069871:intron 60.0%
! GAAGCATCTCCAGCACTGCC+TGG + chr2.1:3876117-3876136 MS.gene069871:CDS 60.0%
CGCCCAAATCCAGGCAGTGC+TGG - chr2.1:3876129-3876148 None:intergenic 65.0%
CGTGGCTGTGGTGGCTGTGA+TGG - chr2.1:3875849-3875868 None:intergenic 65.0%
GGCTGTGGTGGCTGTGATGG+TGG - chr2.1:3875846-3875865 None:intergenic 65.0%
GGTGGCGTGAGTCGTGAGAG+CGG - chr2.1:3875888-3875907 None:intergenic 65.0%
GTCGTGAGAGCGGCTTGAGG+TGG - chr2.1:3875878-3875897 None:intergenic 65.0%
CACAGCCACCACAGCCACGG+CGG + chr2.1:3875850-3875869 MS.gene069871:CDS 70.0%
AGGTGGAGCCGCCGTGGCTG+TGG - chr2.1:3875861-3875880 None:intergenic 75.0%
GGCTTGAGGTGGAGCCGCCG+TGG - chr2.1:3875867-3875886 None:intergenic 75.0%
TGGAGCCGCCGTGGCTGTGG+TGG - chr2.1:3875858-3875877 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 3875775 3877312 3875775 ID=MS.gene069871
chr2.1 mRNA 3875775 3877312 3875775 ID=MS.gene069871.t1;Parent=MS.gene069871
chr2.1 exon 3875775 3876158 3875775 ID=MS.gene069871.t1.exon1;Parent=MS.gene069871.t1
chr2.1 CDS 3875775 3876158 3875775 ID=cds.MS.gene069871.t1;Parent=MS.gene069871.t1
chr2.1 exon 3877301 3877312 3877301 ID=MS.gene069871.t1.exon2;Parent=MS.gene069871.t1
chr2.1 CDS 3877301 3877312 3877301 ID=cds.MS.gene069871.t1;Parent=MS.gene069871.t1
Gene Sequence

>MS.gene069871

ATGGGCCTAAACATGATGCTCAAACGCCGAAGCAAGCTCGCCGGAAAAGCCATTGCCATTCTAATGTTCCACCATCACAGCCACCACAGCCACGGCGGCTCCACCTCAAGCCGCTCTCACGACTCACGCCACCAATTTACCGCATCAAGGGAGTATGAATTCAGCTGCAGCAACACTCCCCATCACTTTTTCCCAATAGGGAAACGTCACCGCAGCAACAACCACAATCACAACAATTTCTTCACTTGCGCTCATACGCCACCTACACAGGACGATGATGTGGCGACAATGAGTGCAATGAAGGCCGTGCTGGAGATGCTTAACAATGATCAAGCAATCGTAGAAGCATCTCCAGCACTGCCTGGATTTGGGCGTAGCCCAACGGTAAGGCAATTGAGGGTGACTGACTCACCATTCCCTCTAAGAGAAGATGATGAGAAGGACGATCAAGTTGATAAGGCTGCTGAAGATTTCATAAACAGGTTTTACAGCCAATTGAGGAAGCAAGATTGATTGATTAAATAATTAATTCAACCTACGTACCTTGTTTAGTTAATAATTAATGTGTGGAATCGTTTGTTGTGAATGCTAAGTAACCATTTTGCATTTTTGGTTATTTTCATGATATATGATCCAGAGTTGATATACGGTACCTACATGTGGGAGATTTATACGTCTCATGTTTGATTTAATTGTTGATTATCAACCTTGTTCAAAAAAAATTGTTGATTATCAACAATAATAATAAGAGAATAAATTACAGATGGAATTAATTAATTGATTATCCACCGCTGCTCTTGTGTTTTTTCTCAAATAAATTACGGCATATAATTCCTTTTCATAATTTCTAACAAAATATATAATCTCATGCCTTAAAAAGAATCTCATATATATGTTTGGTGAGTAACTAATCACCGTGAGCTTAACTCAATTGGTAAAAACAATGTATAATATATGTAAATCTCATACCACCAAAAAGAAGAAAATCACCGTCATCGTGCTCACAAAAACAAAATCACCTTTCTTAATAATAATCACTTTTAAATTCTAAATTTTTTTGGTTGTGTACTTTATTTTAGTTAACTTTACGTACTCTCGCATTTGGAGTGCTTTTATTTTGAGTCTATCTATGAATTGAAGAAAAAGACTACACTTTAAATTCGTATATAAACTTGAAATATGTGTTAAGGGTTCAAACATAAGCTTGTTTTTGTTATTATCTTTCATTATTAATAATAGATAGAAAATATTTGTTTTTTAAACAATAATTGAAATATTTTAGTTAACTTTACGAATTAATTAGCCGGTATATTATGTACTCTAACAAACATGATCGAATCTTTCTGAGGTACCCTTCGTACACATCTTTGTACTAAAAATTTCAATGAATCTTTTGGATTTAGGATATACTCTTGGCTATAAGATAAGCACCTTGCAGAATTTTGGGTTGTAACAACAATATTAAATATTTTTTCTTCTCTTTCTAACTAGTTTCTTCATTTTAATTCTTGCTGTTAACCTCTTAGCCTTTGCCTTAA

Protein sequence

>MS.gene069871.t1

MGLNMMLKRRSKLAGKAIAILMFHHHSHHSHGGSTSSRSHDSRHQFTASREYEFSCSNTPHHFFPIGKRHRSNNHNHNNFFTCAHTPPTQDDDVATMSAMKAVLEMLNNDQAIVEASPALPGFGRSPTPLP