Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85152.t1 | XP_003597685.1 | 93.6 | 188 | 9 | 1 | 1 | 188 | 15 | 199 | 2.20E-74 | 288.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85152.t1 | G7II07 | 93.6 | 188 | 9 | 1 | 1 | 188 | 15 | 199 | 1.6e-74 | 288.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene85152 | -0.812353 | 4.43E-51 | -1.69E-46 |
| MS.gene049171 | MS.gene85152 | -0.827047 | 1.94E-54 | -1.69E-46 |
| MS.gene049217 | MS.gene85152 | -0.802355 | 5.84E-49 | -1.69E-46 |
| MS.gene049222 | MS.gene85152 | -0.831204 | 1.91E-55 | -1.69E-46 |
| MS.gene049285 | MS.gene85152 | -0.815112 | 1.09E-51 | -1.69E-46 |
| MS.gene049320 | MS.gene85152 | -0.827778 | 1.30E-54 | -1.69E-46 |
| MS.gene04932 | MS.gene85152 | 0.813164 | 2.94E-51 | -1.69E-46 |
| MS.gene049549 | MS.gene85152 | 0.837967 | 3.80E-57 | -1.69E-46 |
| MS.gene049633 | MS.gene85152 | -0.819825 | 9.48E-53 | -1.69E-46 |
| MS.gene049634 | MS.gene85152 | 0.801562 | 8.49E-49 | -1.69E-46 |
| MS.gene050163 | MS.gene85152 | -0.815198 | 1.05E-51 | -1.69E-46 |
| MS.gene050165 | MS.gene85152 | -0.821387 | 4.15E-53 | -1.69E-46 |
| MS.gene050170 | MS.gene85152 | -0.830962 | 2.19E-55 | -1.69E-46 |
| MS.gene050323 | MS.gene85152 | -0.811642 | 6.32E-51 | -1.69E-46 |
| MS.gene050333 | MS.gene85152 | -0.803297 | 3.73E-49 | -1.69E-46 |
| MS.gene050338 | MS.gene85152 | 0.814542 | 1.46E-51 | -1.69E-46 |
| MS.gene050444 | MS.gene85152 | -0.847072 | 1.46E-59 | -1.69E-46 |
| MS.gene050504 | MS.gene85152 | -0.826566 | 2.53E-54 | -1.69E-46 |
| MS.gene050577 | MS.gene85152 | -0.844807 | 6.02E-59 | -1.69E-46 |
| MS.gene050612 | MS.gene85152 | -0.836808 | 7.53E-57 | -1.69E-46 |
| MS.gene050699 | MS.gene85152 | -0.813987 | 1.94E-51 | -1.69E-46 |
| MS.gene050747 | MS.gene85152 | -0.801435 | 9.02E-49 | -1.69E-46 |
| MS.gene05081 | MS.gene85152 | -0.806483 | 8.05E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene85152 | -0.822266 | 2.60E-53 | -1.69E-46 |
| MS.gene050871 | MS.gene85152 | -0.819153 | 1.35E-52 | -1.69E-46 |
| MS.gene050904 | MS.gene85152 | -0.830292 | 3.19E-55 | -1.69E-46 |
| MS.gene050986 | MS.gene85152 | -0.800545 | 1.37E-48 | -1.69E-46 |
| MS.gene051138 | MS.gene85152 | 0.836675 | 8.14E-57 | -1.69E-46 |
| MS.gene051317 | MS.gene85152 | -0.85035 | 1.80E-60 | -1.69E-46 |
| MS.gene051439 | MS.gene85152 | 0.803857 | 2.85E-49 | -1.69E-46 |
| MS.gene051440 | MS.gene85152 | 0.812495 | 4.12E-51 | -1.69E-46 |
| MS.gene051517 | MS.gene85152 | 0.810985 | 8.78E-51 | -1.69E-46 |
| MS.gene051651 | MS.gene85152 | 0.844236 | 8.57E-59 | -1.69E-46 |
| MS.gene051838 | MS.gene85152 | -0.819 | 1.46E-52 | -1.69E-46 |
| MS.gene051973 | MS.gene85152 | 0.808935 | 2.42E-50 | -1.69E-46 |
| MS.gene052050 | MS.gene85152 | 0.808089 | 3.68E-50 | -1.69E-46 |
| MS.gene052164 | MS.gene85152 | -0.815094 | 1.10E-51 | -1.69E-46 |
| MS.gene052211 | MS.gene85152 | 0.835175 | 1.96E-56 | -1.69E-46 |
| MS.gene05240 | MS.gene85152 | 0.80484 | 1.78E-49 | -1.69E-46 |
| MS.gene05244 | MS.gene85152 | 0.803062 | 4.17E-49 | -1.69E-46 |
| MS.gene052482 | MS.gene85152 | -0.822416 | 2.40E-53 | -1.69E-46 |
| MS.gene052486 | MS.gene85152 | -0.827374 | 1.62E-54 | -1.69E-46 |
| MS.gene052494 | MS.gene85152 | 0.801594 | 8.36E-49 | -1.69E-46 |
| MS.gene052503 | MS.gene85152 | -0.814993 | 1.16E-51 | -1.69E-46 |
| MS.gene052606 | MS.gene85152 | 0.852503 | 4.42E-61 | -1.69E-46 |
| MS.gene052823 | MS.gene85152 | -0.817817 | 2.71E-52 | -1.69E-46 |
| MS.gene052825 | MS.gene85152 | -0.816055 | 6.74E-52 | -1.69E-46 |
| MS.gene052872 | MS.gene85152 | -0.816523 | 5.29E-52 | -1.69E-46 |
| MS.gene053420 | MS.gene85152 | 0.814613 | 1.41E-51 | -1.69E-46 |
| MS.gene053479 | MS.gene85152 | -0.828394 | 9.21E-55 | -1.69E-46 |
| MS.gene053480 | MS.gene85152 | -0.825939 | 3.56E-54 | -1.69E-46 |
| MS.gene053579 | MS.gene85152 | -0.817547 | 3.12E-52 | -1.69E-46 |
| MS.gene053632 | MS.gene85152 | -0.804527 | 2.07E-49 | -1.69E-46 |
| MS.gene053776 | MS.gene85152 | 0.819328 | 1.23E-52 | -1.69E-46 |
| MS.gene053824 | MS.gene85152 | -0.819881 | 9.20E-53 | -1.69E-46 |
| MS.gene053916 | MS.gene85152 | -0.802019 | 6.84E-49 | -1.69E-46 |
| MS.gene054107 | MS.gene85152 | -0.804836 | 1.78E-49 | -1.69E-46 |
| MS.gene054146 | MS.gene85152 | 0.816874 | 4.42E-52 | -1.69E-46 |
| MS.gene054344 | MS.gene85152 | -0.804028 | 2.63E-49 | -1.69E-46 |
| MS.gene054408 | MS.gene85152 | -0.805774 | 1.13E-49 | -1.69E-46 |
| MS.gene054642 | MS.gene85152 | -0.801469 | 8.87E-49 | -1.69E-46 |
| MS.gene054706 | MS.gene85152 | 0.820525 | 6.55E-53 | -1.69E-46 |
| MS.gene05470 | MS.gene85152 | -0.877945 | 4.18E-69 | -1.69E-46 |
| MS.gene054884 | MS.gene85152 | 0.846528 | 2.05E-59 | -1.69E-46 |
| MS.gene054954 | MS.gene85152 | -0.806883 | 6.62E-50 | -1.69E-46 |
| MS.gene055154 | MS.gene85152 | 0.805259 | 1.46E-49 | -1.69E-46 |
| MS.gene055158 | MS.gene85152 | 0.80332 | 3.69E-49 | -1.69E-46 |
| MS.gene055206 | MS.gene85152 | -0.80148 | 8.83E-49 | -1.69E-46 |
| MS.gene055240 | MS.gene85152 | 0.811499 | 6.79E-51 | -1.69E-46 |
| MS.gene055362 | MS.gene85152 | -0.815245 | 1.02E-51 | -1.69E-46 |
| MS.gene055502 | MS.gene85152 | -0.840616 | 7.81E-58 | -1.69E-46 |
| MS.gene055577 | MS.gene85152 | -0.818376 | 2.02E-52 | -1.69E-46 |
| MS.gene055731 | MS.gene85152 | 0.80452 | 2.08E-49 | -1.69E-46 |
| MS.gene055748 | MS.gene85152 | -0.861263 | 1.16E-63 | -1.69E-46 |
| MS.gene055750 | MS.gene85152 | -0.824432 | 8.10E-54 | -1.69E-46 |
| MS.gene055751 | MS.gene85152 | -0.859822 | 3.17E-63 | -1.69E-46 |
| MS.gene05589 | MS.gene85152 | -0.863044 | 3.30E-64 | -1.69E-46 |
| MS.gene056224 | MS.gene85152 | 0.802711 | 4.93E-49 | -1.69E-46 |
| MS.gene056387 | MS.gene85152 | -0.821474 | 3.96E-53 | -1.69E-46 |
| MS.gene056504 | MS.gene85152 | -0.845389 | 4.19E-59 | -1.69E-46 |
| MS.gene056590 | MS.gene85152 | 0.837706 | 4.44E-57 | -1.69E-46 |
| MS.gene056591 | MS.gene85152 | 0.838445 | 2.86E-57 | -1.69E-46 |
| MS.gene056618 | MS.gene85152 | 0.809784 | 1.59E-50 | -1.69E-46 |
| MS.gene056657 | MS.gene85152 | -0.819304 | 1.25E-52 | -1.69E-46 |
| MS.gene056678 | MS.gene85152 | 0.807022 | 6.19E-50 | -1.69E-46 |
| MS.gene056688 | MS.gene85152 | 0.813693 | 2.25E-51 | -1.69E-46 |
| MS.gene056692 | MS.gene85152 | -0.828763 | 7.50E-55 | -1.69E-46 |
| MS.gene056694 | MS.gene85152 | 0.853529 | 2.25E-61 | -1.69E-46 |
| MS.gene056697 | MS.gene85152 | 0.850251 | 1.92E-60 | -1.69E-46 |
| MS.gene056748 | MS.gene85152 | 0.815139 | 1.08E-51 | -1.69E-46 |
| MS.gene056856 | MS.gene85152 | 0.842563 | 2.40E-58 | -1.69E-46 |
| MS.gene056903 | MS.gene85152 | -0.800451 | 1.43E-48 | -1.69E-46 |
| MS.gene057246 | MS.gene85152 | -0.800327 | 1.52E-48 | -1.69E-46 |
| MS.gene057332 | MS.gene85152 | -0.807118 | 5.91E-50 | -1.69E-46 |
| MS.gene057658 | MS.gene85152 | -0.819904 | 9.09E-53 | -1.69E-46 |
| MS.gene057717 | MS.gene85152 | 0.838599 | 2.61E-57 | -1.69E-46 |
| MS.gene057768 | MS.gene85152 | 0.832794 | 7.71E-56 | -1.69E-46 |
| MS.gene057831 | MS.gene85152 | -0.818002 | 2.46E-52 | -1.69E-46 |
| MS.gene058068 | MS.gene85152 | 0.810211 | 1.29E-50 | -1.69E-46 |
| MS.gene058158 | MS.gene85152 | 0.806168 | 9.38E-50 | -1.69E-46 |
| MS.gene058334 | MS.gene85152 | 0.822032 | 2.94E-53 | -1.69E-46 |
| MS.gene05837 | MS.gene85152 | -0.819137 | 1.36E-52 | -1.69E-46 |
| MS.gene058418 | MS.gene85152 | 0.818227 | 2.19E-52 | -1.69E-46 |
| MS.gene058419 | MS.gene85152 | -0.83819 | 3.33E-57 | -1.69E-46 |
| MS.gene058452 | MS.gene85152 | 0.836735 | 7.86E-57 | -1.69E-46 |
| MS.gene058544 | MS.gene85152 | -0.826728 | 2.31E-54 | -1.69E-46 |
| MS.gene058603 | MS.gene85152 | 0.84984 | 2.50E-60 | -1.69E-46 |
| MS.gene058838 | MS.gene85152 | -0.845964 | 2.92E-59 | -1.69E-46 |
| MS.gene059022 | MS.gene85152 | -0.805531 | 1.28E-49 | -1.69E-46 |
| MS.gene059023 | MS.gene85152 | -0.846302 | 2.37E-59 | -1.69E-46 |
| MS.gene05914 | MS.gene85152 | -0.857581 | 1.48E-62 | -1.69E-46 |
| MS.gene05926 | MS.gene85152 | 0.82911 | 6.18E-55 | -1.69E-46 |
| MS.gene05934 | MS.gene85152 | -0.817378 | 3.40E-52 | -1.69E-46 |
| MS.gene059526 | MS.gene85152 | 0.816278 | 6.00E-52 | -1.69E-46 |
| MS.gene05960 | MS.gene85152 | 0.829418 | 5.21E-55 | -1.69E-46 |
| MS.gene059667 | MS.gene85152 | -0.803199 | 3.91E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene85152 | -0.839373 | 1.65E-57 | -1.69E-46 |
| MS.gene059718 | MS.gene85152 | -0.80356 | 3.29E-49 | -1.69E-46 |
| MS.gene059756 | MS.gene85152 | -0.801499 | 8.75E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene85152 | -0.824347 | 8.48E-54 | -1.69E-46 |
| MS.gene059953 | MS.gene85152 | 0.804961 | 1.68E-49 | -1.69E-46 |
| MS.gene059955 | MS.gene85152 | 0.826498 | 2.62E-54 | -1.69E-46 |
| MS.gene06000 | MS.gene85152 | -0.808363 | 3.21E-50 | -1.69E-46 |
| MS.gene060087 | MS.gene85152 | 0.821025 | 5.03E-53 | -1.69E-46 |
| MS.gene060405 | MS.gene85152 | 0.823106 | 1.66E-53 | -1.69E-46 |
| MS.gene060762 | MS.gene85152 | -0.862778 | 3.98E-64 | -1.69E-46 |
| MS.gene06077 | MS.gene85152 | -0.839522 | 1.51E-57 | -1.69E-46 |
| MS.gene060823 | MS.gene85152 | 0.800269 | 1.56E-48 | -1.69E-46 |
| MS.gene06084 | MS.gene85152 | 0.810243 | 1.27E-50 | -1.69E-46 |
| MS.gene06092 | MS.gene85152 | -0.802582 | 5.24E-49 | -1.69E-46 |
| MS.gene060982 | MS.gene85152 | -0.84219 | 3.01E-58 | -1.69E-46 |
| MS.gene061044 | MS.gene85152 | 0.850794 | 1.35E-60 | -1.69E-46 |
| MS.gene061150 | MS.gene85152 | -0.813044 | 3.12E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85152.t1 | MTR_2g101130 | 96.277 | 188 | 4 | 1 | 1 | 188 | 15 | 199 | 1.29e-134 | 375 |
| MS.gene85152.t1 | MTR_2g101120 | 89.418 | 189 | 12 | 3 | 1 | 189 | 15 | 195 | 9.52e-120 | 337 |
| MS.gene85152.t1 | MTR_8g014030 | 67.196 | 189 | 47 | 6 | 1 | 188 | 15 | 189 | 7.78e-85 | 248 |
| MS.gene85152.t1 | MTR_8g014010 | 62.766 | 188 | 51 | 7 | 1 | 186 | 15 | 185 | 7.42e-73 | 218 |
| MS.gene85152.t1 | MTR_8g014020 | 63.087 | 149 | 40 | 6 | 41 | 188 | 1 | 135 | 9.49e-58 | 178 |
| MS.gene85152.t1 | MTR_6g088525 | 42.268 | 194 | 72 | 11 | 2 | 188 | 19 | 179 | 1.07e-33 | 119 |
| MS.gene85152.t1 | MTR_6g088545 | 39.378 | 193 | 80 | 7 | 2 | 188 | 19 | 180 | 9.02e-33 | 116 |
| MS.gene85152.t1 | MTR_6g088565 | 38.342 | 193 | 82 | 7 | 2 | 188 | 19 | 180 | 4.57e-31 | 112 |
| MS.gene85152.t1 | MTR_0016s0060 | 39.894 | 188 | 75 | 7 | 8 | 188 | 1 | 157 | 5.50e-30 | 108 |
| MS.gene85152.t1 | MTR_6g088505 | 39.691 | 194 | 78 | 9 | 2 | 188 | 19 | 180 | 5.55e-30 | 109 |
| MS.gene85152.t1 | MTR_0016s0050 | 39.888 | 178 | 68 | 8 | 18 | 188 | 1 | 146 | 1.79e-26 | 99.4 |
| MS.gene85152.t1 | MTR_8g102660 | 36.788 | 193 | 83 | 11 | 1 | 188 | 17 | 175 | 2.08e-24 | 94.7 |
| MS.gene85152.t1 | MTR_7g084690 | 33.649 | 211 | 98 | 13 | 1 | 189 | 15 | 205 | 3.69e-16 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85152.t1 | AT1G52140 | 47.000 | 200 | 82 | 7 | 1 | 188 | 14 | 201 | 1.27e-49 | 160 |
| MS.gene85152.t1 | AT3G16330 | 42.647 | 204 | 80 | 8 | 1 | 188 | 14 | 196 | 1.61e-40 | 136 |
| MS.gene85152.t1 | AT4G29110 | 37.895 | 190 | 82 | 6 | 1 | 188 | 28 | 183 | 1.41e-25 | 98.2 |
Find 53 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCCCTTGATGCGGTAAAT+TGG | 0.283815 | 2.4:-3489797 | None:intergenic |
| TGCGGTGACGTTTCCCTATT+GGG | 0.299531 | 2.4:-3489857 | None:intergenic |
| TCTCCAGCACTGCCTGGATT+CGG | 0.302018 | 2.4:+3490014 | MS.gene85152:CDS |
| CTGCGGTGACGTTTCCCTAT+TGG | 0.308179 | 2.4:-3489858 | None:intergenic |
| CTCCAGCACTGCCTGGATTC+GGG | 0.322990 | 2.4:+3490015 | MS.gene85152:CDS |
| ATCAAGCTTGCTTCCTCAAT+TGG | 0.341340 | 2.4:-3490157 | None:intergenic |
| CCCTCAATTGCCTTACCATT+GGG | 0.354815 | 2.4:-3490043 | None:intergenic |
| TTCTCATCATCTTCTCTTAG+AGG | 0.359691 | 2.4:-3490083 | None:intergenic |
| CTGCTGAAGATTTCATAAAC+AGG | 0.373468 | 2.4:+3490126 | MS.gene85152:CDS |
| CGCCCGAATCCAGGCAGTGC+TGG | 0.394166 | 2.4:-3490017 | None:intergenic |
| GGCGTTTGAGCATCATGTTT+AGG | 0.451131 | 2.4:-3489671 | None:intergenic |
| CGTGGCTGTGGTGGCTGTGA+TGG | 0.466817 | 2.4:-3489737 | None:intergenic |
| ACCCTCAATTGCCTTACCAT+TGG | 0.467623 | 2.4:-3490044 | None:intergenic |
| GTCAAAGGGCAAACTAATGA+TGG | 0.467955 | 2.4:+3489647 | MS.gene85152:CDS |
| GCCTTCTTCATGTTGAGAAA+AGG | 0.468789 | 2.4:+3489621 | MS.gene85152:CDS |
| TGAAGAAATTGTTGTGATTG+TGG | 0.472141 | 2.4:-3489887 | None:intergenic |
| AGGTGGAGCCGCCGTGGCTG+TGG | 0.473691 | 2.4:-3489749 | None:intergenic |
| CCCTTGATGCGGTAAATTGG+TGG | 0.485419 | 2.4:-3489794 | None:intergenic |
| GCCCAATGGTAAGGCAATTG+AGG | 0.485585 | 2.4:+3490042 | MS.gene85152:CDS |
| GAAGCATCTCCAGCACTGCC+TGG | 0.487263 | 2.4:+3490008 | MS.gene85152:CDS |
| GAGTGCAATGAAGGCCGTGC+TGG | 0.499574 | 2.4:+3489956 | MS.gene85152:CDS |
| CATCACAGCCACCACAGCCA+CGG | 0.499967 | 2.4:+3489738 | MS.gene85152:CDS |
| TCATCTTCTCTTAGAGGGAA+TGG | 0.502641 | 2.4:-3490077 | None:intergenic |
| CCCAATGGTAAGGCAATTGA+GGG | 0.507064 | 2.4:+3490043 | MS.gene85152:CDS |
| GTCGTGAGAGCGGCTTGAGG+TGG | 0.508313 | 2.4:-3489766 | None:intergenic |
| ATTGGGCTACGCCCGAATCC+AGG | 0.513644 | 2.4:-3490026 | None:intergenic |
| GAAGGACGATCAAGTTGATA+AGG | 0.519057 | 2.4:+3490103 | MS.gene85152:CDS |
| GGCTGTGGTGGCTGTGATGG+TGG | 0.525762 | 2.4:-3489734 | None:intergenic |
| TCTCATCATCTTCTCTTAGA+GGG | 0.530370 | 2.4:-3490082 | None:intergenic |
| TTGAGAAAAGGCATGTCAAA+GGG | 0.534014 | 2.4:+3489633 | MS.gene85152:CDS |
| GCCACCAATTTACCGCATCA+AGG | 0.534674 | 2.4:+3489793 | MS.gene85152:CDS |
| TGGAGCCGCCGTGGCTGTGG+TGG | 0.543982 | 2.4:-3489746 | None:intergenic |
| GTTGAGAAAAGGCATGTCAA+AGG | 0.559027 | 2.4:+3489632 | MS.gene85152:CDS |
| GTGGAACATTAGATTGGCAA+TGG | 0.565737 | 2.4:-3489715 | None:intergenic |
| AAACGCCGAAGCAAGCTCGC+CGG | 0.566114 | 2.4:+3489687 | MS.gene85152:CDS |
| GTGATGGTGGAACATTAGAT+TGG | 0.566670 | 2.4:-3489721 | None:intergenic |
| CACTCATACGCCACCTACAC+AGG | 0.567460 | 2.4:+3489914 | MS.gene85152:CDS |
| TCGGGCGTAGCCCAATGGTA+AGG | 0.575007 | 2.4:+3490033 | MS.gene85152:CDS |
| GGCGACAATGAGTGCAATGA+AGG | 0.584967 | 2.4:+3489947 | MS.gene85152:CDS |
| TGGATTCGGGCGTAGCCCAA+TGG | 0.585672 | 2.4:+3490028 | MS.gene85152:CDS |
| ACATCATCGTCCTGTGTAGG+TGG | 0.587034 | 2.4:-3489924 | None:intergenic |
| TCAAAGGGCAAACTAATGAT+GGG | 0.588410 | 2.4:+3489648 | MS.gene85152:CDS |
| CCACCAATTTACCGCATCAA+GGG | 0.590913 | 2.4:+3489794 | MS.gene85152:CDS |
| TCTAAGAGAAGATGATGAGA+AGG | 0.594411 | 2.4:+3490085 | MS.gene85152:CDS |
| GGCTTGAGGTGGAGCCGCCG+TGG | 0.620681 | 2.4:-3489755 | None:intergenic |
| GGTGGCGTGAGTCGTGAGAG+CGG | 0.626926 | 2.4:-3489776 | None:intergenic |
| TGTGATTGTGGTTGTTGCTG+CGG | 0.635520 | 2.4:-3489875 | None:intergenic |
| GCCACATCATCGTCCTGTGT+AGG | 0.639849 | 2.4:-3489927 | None:intergenic |
| ATTGTTAAGCATCTCCAGCA+CGG | 0.641871 | 2.4:-3489970 | None:intergenic |
| CACAGCCACCACAGCCACGG+CGG | 0.652559 | 2.4:+3489741 | MS.gene85152:CDS |
| ACCTACACAGGACGATGATG+TGG | 0.656484 | 2.4:+3489926 | MS.gene85152:CDS |
| TGAGTCGTGAGAGCGGCTTG+AGG | 0.683296 | 2.4:-3489769 | None:intergenic |
| GAATTCATACTCCCTTGATG+CGG | 0.696823 | 2.4:-3489805 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGAAGAAATTGTTGTGATTG+TGG | - | chr2.4:3489890-3489909 | None:intergenic | 30.0% | |
| CTGCTGAAGATTTCATAAAC+AGG | + | chr2.4:3490126-3490145 | MS.gene85152:CDS | 35.0% | |
| TCAAAGGGCAAACTAATGAT+GGG | + | chr2.4:3489648-3489667 | MS.gene85152:CDS | 35.0% | |
| TCTAAGAGAAGATGATGAGA+AGG | + | chr2.4:3490085-3490104 | MS.gene85152:CDS | 35.0% | |
| TCTCATCATCTTCTCTTAGA+GGG | - | chr2.4:3490085-3490104 | None:intergenic | 35.0% | |
| TTCTCATCATCTTCTCTTAG+AGG | - | chr2.4:3490086-3490105 | None:intergenic | 35.0% | |
| ! | TTGAGAAAAGGCATGTCAAA+GGG | + | chr2.4:3489633-3489652 | MS.gene85152:CDS | 35.0% |
| ATTGTTAAGCATCTCCAGCA+CGG | - | chr2.4:3489973-3489992 | None:intergenic | 40.0% | |
| CCCTATTGGGAAAAAGTGAT+GGG | - | chr2.4:3489847-3489866 | None:intergenic | 40.0% | |
| CCTATTGGGAAAAAGTGATG+GGG | - | chr2.4:3489846-3489865 | None:intergenic | 40.0% | |
| GAATTCATACTCCCTTGATG+CGG | - | chr2.4:3489808-3489827 | None:intergenic | 40.0% | |
| GCCTTCTTCATGTTGAGAAA+AGG | + | chr2.4:3489621-3489640 | MS.gene85152:CDS | 40.0% | |
| GTCAAAGGGCAAACTAATGA+TGG | + | chr2.4:3489647-3489666 | MS.gene85152:CDS | 40.0% | |
| GTGGAACATTAGATTGGCAA+TGG | - | chr2.4:3489718-3489737 | None:intergenic | 40.0% | |
| TCATCTTCTCTTAGAGGGAA+TGG | - | chr2.4:3490080-3490099 | None:intergenic | 40.0% | |
| TCCCTATTGGGAAAAAGTGA+TGG | - | chr2.4:3489848-3489867 | None:intergenic | 40.0% | |
| ! | ACAGGTTTTACAGCCAATTG+AGG | + | chr2.4:3490144-3490163 | MS.gene85152:CDS | 40.0% |
| ! | GCCTTTTCTCAACATGAAGA+AGG | - | chr2.4:3489625-3489644 | None:intergenic | 40.0% |
| ! | GTTGAGAAAAGGCATGTCAA+AGG | + | chr2.4:3489632-3489651 | MS.gene85152:CDS | 40.0% |
| ! | TAGATTGGCAATGGCTTTTC+CGG | - | chr2.4:3489709-3489728 | None:intergenic | 40.0% |
| !! | GAAGGACGATCAAGTTGATA+AGG | + | chr2.4:3490103-3490122 | MS.gene85152:CDS | 40.0% |
| !! | GTGATGGTGGAACATTAGAT+TGG | - | chr2.4:3489724-3489743 | None:intergenic | 40.0% |
| !!! | CCCATCACTTTTTCCCAATA+GGG | + | chr2.4:3489844-3489863 | MS.gene85152:CDS | 40.0% |
| ACCCTCAATTGCCTTACCAT+TGG | - | chr2.4:3490047-3490066 | None:intergenic | 45.0% | |
| ACTCCCTTGATGCGGTAAAT+TGG | - | chr2.4:3489800-3489819 | None:intergenic | 45.0% | |
| CCACCAATTTACCGCATCAA+GGG | + | chr2.4:3489794-3489813 | MS.gene85152:CDS | 45.0% | |
| CCCAATGGTAAGGCAATTGA+GGG | + | chr2.4:3490043-3490062 | MS.gene85152:CDS | 45.0% | |
| CCCTCAATTGCCTTACCATT+GGG | - | chr2.4:3490046-3490065 | None:intergenic | 45.0% | |
| GGCGTTTGAGCATCATGTTT+AGG | - | chr2.4:3489674-3489693 | None:intergenic | 45.0% | |
| TGTGATTGTGGTTGTTGCTG+CGG | - | chr2.4:3489878-3489897 | None:intergenic | 45.0% | |
| !!! | CCCCATCACTTTTTCCCAAT+AGG | + | chr2.4:3489843-3489862 | MS.gene85152:CDS | 45.0% |
| ACATCATCGTCCTGTGTAGG+TGG | - | chr2.4:3489927-3489946 | None:intergenic | 50.0% | |
| ACCTACACAGGACGATGATG+TGG | + | chr2.4:3489926-3489945 | MS.gene85152:CDS | 50.0% | |
| CCCTTGATGCGGTAAATTGG+TGG | - | chr2.4:3489797-3489816 | None:intergenic | 50.0% | |
| GCCACCAATTTACCGCATCA+AGG | + | chr2.4:3489793-3489812 | MS.gene85152:CDS | 50.0% | |
| GCCCAATGGTAAGGCAATTG+AGG | + | chr2.4:3490042-3490061 | MS.gene85152:CDS | 50.0% | |
| GGCGACAATGAGTGCAATGA+AGG | + | chr2.4:3489947-3489966 | MS.gene85152:CDS | 50.0% | |
| TGCGGTGACGTTTCCCTATT+GGG | - | chr2.4:3489860-3489879 | None:intergenic | 50.0% | |
| CACTCATACGCCACCTACAC+AGG | + | chr2.4:3489914-3489933 | MS.gene85152:CDS | 55.0% | |
| CTGCGGTGACGTTTCCCTAT+TGG | - | chr2.4:3489861-3489880 | None:intergenic | 55.0% | |
| GCCACATCATCGTCCTGTGT+AGG | - | chr2.4:3489930-3489949 | None:intergenic | 55.0% | |
| TCTCCAGCACTGCCTGGATT+CGG | + | chr2.4:3490014-3490033 | MS.gene85152:CDS | 55.0% | |
| !! | CTTTTCCGGCGAGCTTGCTT+CGG | - | chr2.4:3489695-3489714 | None:intergenic | 55.0% |
| AAACGCCGAAGCAAGCTCGC+CGG | + | chr2.4:3489687-3489706 | MS.gene85152:CDS | 60.0% | |
| ATTGGGCTACGCCCGAATCC+AGG | - | chr2.4:3490029-3490048 | None:intergenic | 60.0% | |
| CATCACAGCCACCACAGCCA+CGG | + | chr2.4:3489738-3489757 | MS.gene85152:CDS | 60.0% | |
| CTCCAGCACTGCCTGGATTC+GGG | + | chr2.4:3490015-3490034 | MS.gene85152:CDS | 60.0% | |
| GAGTGCAATGAAGGCCGTGC+TGG | + | chr2.4:3489956-3489975 | MS.gene85152:CDS | 60.0% | |
| TCGGGCGTAGCCCAATGGTA+AGG | + | chr2.4:3490033-3490052 | MS.gene85152:CDS | 60.0% | |
| TGAGTCGTGAGAGCGGCTTG+AGG | - | chr2.4:3489772-3489791 | None:intergenic | 60.0% | |
| TGGATTCGGGCGTAGCCCAA+TGG | + | chr2.4:3490028-3490047 | MS.gene85152:CDS | 60.0% | |
| ! | GAAGCATCTCCAGCACTGCC+TGG | + | chr2.4:3490008-3490027 | MS.gene85152:CDS | 60.0% |
| CGTGGCTGTGGTGGCTGTGA+TGG | - | chr2.4:3489740-3489759 | None:intergenic | 65.0% | |
| GGCTGTGGTGGCTGTGATGG+TGG | - | chr2.4:3489737-3489756 | None:intergenic | 65.0% | |
| GGTGGCGTGAGTCGTGAGAG+CGG | - | chr2.4:3489779-3489798 | None:intergenic | 65.0% | |
| GTCGTGAGAGCGGCTTGAGG+TGG | - | chr2.4:3489769-3489788 | None:intergenic | 65.0% | |
| CACAGCCACCACAGCCACGG+CGG | + | chr2.4:3489741-3489760 | MS.gene85152:CDS | 70.0% | |
| CGCCCGAATCCAGGCAGTGC+TGG | - | chr2.4:3490020-3490039 | None:intergenic | 70.0% | |
| AGGTGGAGCCGCCGTGGCTG+TGG | - | chr2.4:3489752-3489771 | None:intergenic | 75.0% | |
| GGCTTGAGGTGGAGCCGCCG+TGG | - | chr2.4:3489758-3489777 | None:intergenic | 75.0% | |
| TGGAGCCGCCGTGGCTGTGG+TGG | - | chr2.4:3489749-3489768 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 3489609 | 3490178 | 3489609 | ID=MS.gene85152 |
| chr2.4 | mRNA | 3489609 | 3490178 | 3489609 | ID=MS.gene85152.t1;Parent=MS.gene85152 |
| chr2.4 | exon | 3489609 | 3490178 | 3489609 | ID=MS.gene85152.t1.exon1;Parent=MS.gene85152.t1 |
| chr2.4 | CDS | 3489609 | 3490178 | 3489609 | ID=cds.MS.gene85152.t1;Parent=MS.gene85152.t1 |
| Gene Sequence |
| Protein sequence |
