AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38127


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38127.t1 MTR_5g098530 92.708 96 7 0 1 96 1 96 1.26e-52 162
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38127.t1 AT4G13500 73.214 56 15 0 41 96 48 103 2.46e-24 91.3
MS.gene38127.t1 AT2G05310 71.429 56 16 0 41 96 48 103 3.48e-24 90.9

Find 24 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCTTATATTATCTTATT+TGG 0.144829 5.1:-79173234 MS.gene38127:intron
CGCTTGCGGCAGTGTAATTT+TGG 0.238006 5.1:-79171978 MS.gene38127:CDS
ATCAAAGTTTGTGCTCAATT+AGG 0.317774 5.1:-79173592 MS.gene38127:intron
TTTGTTATTACATACTGTTC+TGG 0.320587 5.1:+79173377 None:intergenic
TGAGAAAGTGAAGATGATTT+AGG 0.339562 5.1:+79173649 None:intergenic
ATACCAAATAAGATAATATA+AGG 0.355873 5.1:+79173231 None:intergenic
TAAGGAAGTGGCGTCATCAT+TGG 0.409790 5.1:+79173249 None:intergenic
TACACTGCCGCAAGCGACTA+AGG 0.429889 5.1:+79171985 None:intergenic
AGAAGATAGAAGAGTTACTT+TGG 0.480230 5.1:+79173699 None:intergenic
TACAGATATTGGCAGTCAGC+AGG 0.495903 5.1:-79173291 MS.gene38127:intron
TAGAAGAGTTACTTTGGCTA+TGG 0.509481 5.1:+79173705 None:intergenic
AAGAGTTACTTTGGCTATGG+TGG 0.517669 5.1:+79173708 None:intergenic
ATCTACACCTTAGTCGCTTG+CGG 0.523454 5.1:-79171992 MS.gene38127:CDS
TCACTAACTTTCACTGCGTA+GGG 0.546971 5.1:+79171902 None:intergenic
AGCACAAACTTTGATACCAA+GGG 0.547969 5.1:+79173600 None:intergenic
AGCGACTAAGGTGTAGATCA+AGG 0.555002 5.1:+79171997 None:intergenic
AATAAGATAATATAAGGAAG+TGG 0.555321 5.1:+79173237 None:intergenic
GCAGAATACAATGAATTCAC+CGG 0.576375 5.1:-79171956 MS.gene38127:CDS
TTCACTAACTTTCACTGCGT+AGG 0.589826 5.1:+79171901 None:intergenic
CGAGATGAAGAAACCAAGAA+AGG 0.593391 5.1:-79173434 MS.gene38127:CDS
CAGTCAGCAGGAGAGAGAGA+AGG 0.609827 5.1:-79173279 MS.gene38127:CDS
CTGAATGTTAGTCAAGCTAC+CGG 0.614514 5.1:+79171937 None:intergenic
GAGCACAAACTTTGATACCA+AGG 0.618661 5.1:+79173599 None:intergenic
CTAACTTTCACTGCGTAGGG+AGG 0.636821 5.1:+79171905 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAACTAGATA+TGG - chr5.1:79173227-79173246 MS.gene38127:intron 10.0%
!!! TTAGACAATATTTTTTTATA+AGG - chr5.1:79173327-79173346 MS.gene38127:intron 10.0%
!! AAATTGTTTATAAATATGAC+TGG + chr5.1:79172689-79172708 None:intergenic 15.0%
!! ATACCAAATAAGATAATATA+AGG + chr5.1:79172376-79172395 None:intergenic 15.0%
!! GTTATACTATATGATTAATA+TGG - chr5.1:79172117-79172136 MS.gene38127:intron 15.0%
!!! TAGTTTTTTTTTTTTTTGAG+AGG + chr5.1:79173223-79173242 None:intergenic 15.0%
!! AAAAAAAAAACTAGATATGG+AGG - chr5.1:79173230-79173249 MS.gene38127:intron 20.0%
!! AATAAGATAATATAAGGAAG+TGG + chr5.1:79172370-79172389 None:intergenic 20.0%
!! CTTCCTTATATTATCTTATT+TGG - chr5.1:79172370-79172389 MS.gene38127:intron 20.0%
!! TACTATTAGTGAAATTGTTA+AGG - chr5.1:79173103-79173122 MS.gene38127:intron 20.0%
!! TGTTGGTATATATTTGTATT+AGG - chr5.1:79172143-79172162 MS.gene38127:intron 20.0%
!!! AATTTTGTTTTGAACATCAT+CGG - chr5.1:79173040-79173059 MS.gene38127:intron 20.0%
!!! TAAATGTTCAATTTTGTAGA+TGG + chr5.1:79172853-79172872 None:intergenic 20.0%
!!! TTACTTTTTCACATATGTAA+CGG - chr5.1:79172581-79172600 MS.gene38127:intron 20.0%
! AAGAAGAATATGTAATAAGG+AGG + chr5.1:79173467-79173486 None:intergenic 25.0%
! AGAAGAATATGTAATAAGGA+GGG + chr5.1:79173466-79173485 None:intergenic 25.0%
! CAAAACATATACATATCGTT+TGG - chr5.1:79172722-79172741 MS.gene38127:intron 25.0%
! CACTTTGATTAGTAAAAACA+TGG + chr5.1:79172278-79172297 None:intergenic 25.0%
! TATGATTAATATGGTTGTGT+TGG - chr5.1:79172126-79172145 MS.gene38127:intron 25.0%
! TGGTATATATTTGTATTAGG+TGG - chr5.1:79172146-79172165 MS.gene38127:intron 25.0%
!! TTTTGTAAACTATTGCACAA+CGG + chr5.1:79173283-79173302 None:intergenic 25.0%
!! TTTTGTAAATGGTTGGATTA+AGG - chr5.1:79172426-79172445 MS.gene38127:intron 25.0%
!!! AAATGAAGTTTTGAAGGTAA+GGG + chr5.1:79171932-79171951 None:intergenic 25.0%
!!! AGAAGTGCTTACAATAAAAT+TGG + chr5.1:79172516-79172535 None:intergenic 25.0%
!!! TTTCTTTTTTTCTCCTTTCT+TGG + chr5.1:79172186-79172205 None:intergenic 25.0%
!!! TTTGTTATTACATACTGTTC+TGG + chr5.1:79172230-79172249 None:intergenic 25.0%
AGAAGATAGAAGAGTTACTT+TGG + chr5.1:79171908-79171927 None:intergenic 30.0%
ATCAAAGTTTGTGCTCAATT+AGG - chr5.1:79172012-79172031 MS.gene38127:intron 30.0%
ATTTGTGATGCTACAGATAT+TGG - chr5.1:79172302-79172321 MS.gene38127:intron 30.0%
GCGAAGAAGAATATGTAATA+AGG + chr5.1:79173470-79173489 None:intergenic 30.0%
TAGATGGATAACTAACAAGT+GGG + chr5.1:79172837-79172856 None:intergenic 30.0%
TCTCAATCCTAAAGAAATCA+GGG - chr5.1:79173137-79173156 MS.gene38127:intron 30.0%
TGAGAAAGTGAAGATGATTT+AGG + chr5.1:79171958-79171977 None:intergenic 30.0%
TTCTCAATCCTAAAGAAATC+AGG - chr5.1:79173136-79173155 MS.gene38127:intron 30.0%
!! AGGAAGAAATGAAGTTTTGA+AGG + chr5.1:79171938-79171957 None:intergenic 30.0%
!! TTAGCCATTGCTTTTGTAAA+TGG - chr5.1:79172415-79172434 MS.gene38127:intron 30.0%
!!! AATGAAGTTTTGAAGGTAAG+GGG + chr5.1:79171931-79171950 None:intergenic 30.0%
!!! GAAATGAAGTTTTGAAGGTA+AGG + chr5.1:79171933-79171952 None:intergenic 30.0%
AAAATCCATGGATGCAGAAT+TGG + chr5.1:79172940-79172959 None:intergenic 35.0%
AAATCCATGGATGCAGAATT+GGG + chr5.1:79172939-79172958 None:intergenic 35.0%
AAGAATCCCCTGATTTCTTT+AGG + chr5.1:79173147-79173166 None:intergenic 35.0%
AAGGATAGACAAATGTGAGT+AGG - chr5.1:79173346-79173365 MS.gene38127:intron 35.0%
AGCACAAACTTTGATACCAA+GGG + chr5.1:79172007-79172026 None:intergenic 35.0%
AGGTACCAATTAGATAGATG+AGG - chr5.1:79172446-79172465 MS.gene38127:intron 35.0%
CCAACCATTTACAAAAGCAA+TGG + chr5.1:79172422-79172441 None:intergenic 35.0%
CTACACAAATAGTCAACTAC+TGG + chr5.1:79173260-79173279 None:intergenic 35.0%
CTCAATCCTAAAGAAATCAG+GGG - chr5.1:79173138-79173157 MS.gene38127:intron 35.0%
CTTGCATCACATTGATCAAT+AGG - chr5.1:79173068-79173087 MS.gene38127:intron 35.0%
GACAAGTTCCATTCAAAGAA+TGG + chr5.1:79173556-79173575 None:intergenic 35.0%
GCAATGGCTAAGATTACAAA+AGG + chr5.1:79172406-79172425 None:intergenic 35.0%
GCAGAATACAATGAATTCAC+CGG - chr5.1:79173648-79173667 MS.gene38127:CDS 35.0%
GGATTCATATGAAGTTATGC+AGG + chr5.1:79173202-79173221 None:intergenic 35.0%
GTAGATGGATAACTAACAAG+TGG + chr5.1:79172838-79172857 None:intergenic 35.0%
GTGAGAATGAACACATTACA+AGG + chr5.1:79172494-79172513 None:intergenic 35.0%
TGTGACCTCATCTATCTAAT+TGG + chr5.1:79172454-79172473 None:intergenic 35.0%
! AATTCTGCATCCATGGATTT+TGG - chr5.1:79172939-79172958 MS.gene38127:intron 35.0%
! ATACCAAGGGAAGTAGTTTT+TGG + chr5.1:79171994-79172013 None:intergenic 35.0%
! ATGAAAACACGACTAGTTGA+TGG - chr5.1:79172888-79172907 MS.gene38127:intron 35.0%
! ATTCTGCATCCATGGATTTT+GGG - chr5.1:79172940-79172959 MS.gene38127:intron 35.0%
! GTCACGTAATTTTGTTCATG+AGG + chr5.1:79172796-79172815 None:intergenic 35.0%
! TAGAAGAGTTACTTTGGCTA+TGG + chr5.1:79171902-79171921 None:intergenic 35.0%
!! ACACATTACAAGGAAGTGTT+TGG + chr5.1:79172484-79172503 None:intergenic 35.0%
!! CCATTGCTTTTGTAAATGGT+TGG - chr5.1:79172419-79172438 MS.gene38127:intron 35.0%
!! TTACAAGGAAGTGTTTGGTT+TGG + chr5.1:79172479-79172498 None:intergenic 35.0%
!!! GTTTTTGTTGTTCCAACGAT+AGG - chr5.1:79173576-79173595 MS.gene38127:intron 35.0%
AAGGATTGATGTCCTATCGT+TGG + chr5.1:79173591-79173610 None:intergenic 40.0%
CGAGATGAAGAAACCAAGAA+AGG - chr5.1:79172170-79172189 MS.gene38127:intron 40.0%
CTGAATGTTAGTCAAGCTAC+CGG + chr5.1:79173670-79173689 None:intergenic 40.0%
CTGGATCACCATTCTTTGAA+TGG - chr5.1:79173545-79173564 MS.gene38127:intron 40.0%
GAACTGGATATGATACATGC+TGG + chr5.1:79172636-79172655 None:intergenic 40.0%
GACAAATGTGAGTAGGTTAC+TGG - chr5.1:79173353-79173372 MS.gene38127:intron 40.0%
GAGCACAAACTTTGATACCA+AGG + chr5.1:79172008-79172027 None:intergenic 40.0%
GCATGTATCATATCCAGTTC+CGG - chr5.1:79172636-79172655 MS.gene38127:intron 40.0%
TATTCTTACTCGTCCTCTAC+CGG + chr5.1:79172658-79172677 None:intergenic 40.0%
TCACTAACTTTCACTGCGTA+GGG + chr5.1:79173705-79173724 None:intergenic 40.0%
TCTCCAAAAACTACTTCCCT+TGG - chr5.1:79171988-79172007 MS.gene38127:CDS 40.0%
TGTAAAAGCCCCAAAATCCA+TGG + chr5.1:79172952-79172971 None:intergenic 40.0%
TTCACTAACTTTCACTGCGT+AGG + chr5.1:79173706-79173725 None:intergenic 40.0%
! AAGAGTTACTTTGGCTATGG+TGG + chr5.1:79171899-79171918 None:intergenic 40.0%
! TTCTGCATCCATGGATTTTG+GGG - chr5.1:79172941-79172960 MS.gene38127:intron 40.0%
!! AAGGGAAGTAGTTTTTGGAG+AGG + chr5.1:79171989-79172008 None:intergenic 40.0%
!! AGTAGTTTTTGGAGAGGCAA+TGG + chr5.1:79171983-79172002 None:intergenic 40.0%
ACTCAGCTTAACTCTTCAGC+AGG - chr5.1:79172992-79173011 MS.gene38127:intron 45.0%
AGCGACTAAGGTGTAGATCA+AGG + chr5.1:79173610-79173629 None:intergenic 45.0%
ATCATATCCAGTTCCGGTAG+AGG - chr5.1:79172642-79172661 MS.gene38127:intron 45.0%
ATCTACACCTTAGTCGCTTG+CGG - chr5.1:79173612-79173631 MS.gene38127:CDS 45.0%
CTCAGCTTAACTCTTCAGCA+GGG - chr5.1:79172993-79173012 MS.gene38127:intron 45.0%
TAAGGAAGTGGCGTCATCAT+TGG + chr5.1:79172358-79172377 None:intergenic 45.0%
TACAGATATTGGCAGTCAGC+AGG - chr5.1:79172313-79172332 MS.gene38127:intron 45.0%
AGAGAGAGAGAGAGAGAGAG+TGG + chr5.1:79173492-79173511 None:intergenic 50.0%
CTAACTTTCACTGCGTAGGG+AGG + chr5.1:79173702-79173721 None:intergenic 50.0%
CTCTCCCAATTCTGCATCCA+TGG - chr5.1:79172932-79172951 MS.gene38127:intron 50.0%
GACTCTCGCTCTCTACAATC+TGG - chr5.1:79173526-79173545 MS.gene38127:intron 50.0%
GCTTAACTCTTCAGCAGGGT+AGG - chr5.1:79172997-79173016 MS.gene38127:intron 50.0%
! CGCTTGCGGCAGTGTAATTT+TGG - chr5.1:79173626-79173645 MS.gene38127:CDS 50.0%
CAGTCAGCAGGAGAGAGAGA+AGG - chr5.1:79172325-79172344 MS.gene38127:intron 55.0%
TACACTGCCGCAAGCGACTA+AGG + chr5.1:79173622-79173641 None:intergenic 55.0%
TACTCGTCCTCTACCGGAAC+TGG + chr5.1:79172652-79172671 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 79171892 79173734 79171892 ID=MS.gene38127
chr5.1 mRNA 79171892 79173734 79171892 ID=MS.gene38127.t1;Parent=MS.gene38127
chr5.1 exon 79173593 79173734 79173593 ID=MS.gene38127.t1.exon1;Parent=MS.gene38127.t1
chr5.1 CDS 79173593 79173734 79173593 ID=cds.MS.gene38127.t1;Parent=MS.gene38127.t1
chr5.1 exon 79173392 79173461 79173392 ID=MS.gene38127.t1.exon2;Parent=MS.gene38127.t1
chr5.1 CDS 79173392 79173461 79173392 ID=cds.MS.gene38127.t1;Parent=MS.gene38127.t1
chr5.1 exon 79173235 79173308 79173235 ID=MS.gene38127.t1.exon3;Parent=MS.gene38127.t1
chr5.1 CDS 79173235 79173308 79173235 ID=cds.MS.gene38127.t1;Parent=MS.gene38127.t1
chr5.1 exon 79171892 79172028 79171892 ID=MS.gene38127.t1.exon4;Parent=MS.gene38127.t1
chr5.1 CDS 79171892 79172028 79171892 ID=cds.MS.gene38127.t1;Parent=MS.gene38127.t1
Gene Sequence

>MS.gene38127

ATGGCCACCATAGCCAAAGTAACTCTTCTATCTTCTCCCCTTACCTTCAAAACTTCATTTCTTCCTAAATCATCTTCACTTTCTCACACCATTGCCTCTCCAAAAACTACTTCCCTTGGTATCAAAGTTTGTGCTCAATTAGGTAACATTAAAACTCTAGCTTCTAATTATTTAATTTTCATGCAAACATTTTGATTCATTATTTAATTTTTTTTAATTTATTTAGTTATACTATATGATTAATATGGTTGTGTTGGTATATATTTGTATTAGGTGGTCGAGATGAAGAAACCAAGAAAGGAGAAAAAAAGAAATTTATAACTAGAGAACAAGAACCAGAACAGTATGTAATAACAAATTAATAACAATTAATTAATTTAATTCCATGTTTTTACTAATCAAAGTGTAATATTTGTGATGCTACAGATATTGGCAGTCAGCAGGAGAGAGAGAAGGAGAGAATCCAATGATGACGCCACTTCCTTATATTATCTTATTTGGTATGTCAACTCCTTTTGTAATCTTAGCCATTGCTTTTGTAAATGGTTGGATTAAGGTACCAATTAGATAGATGAGGTCACACACCAAACCAAACACTTCCTTGTAATGTGTTCATTCTCACCAATTTTATTGTAAGCACTTCTCACTACTCATTAGTAGTAGTTCTCAAGCTTGAGTAAATAATTTATTTACTTTTTCACATATGTAACGGTACAAATATTATGTCATCAATATTCAATTCCAGCATGTATCATATCCAGTTCCGGTAGAGGACGAGTAAGAATATACTCTATCCAGTCATATTTATAAACAATTTTTGTTTTTCATGTCAAAACATATACATATCGTTTGGTAAAGTAATTTAAATGTACGTGCAGTGTGTAATAGTATAGAACAACGACCTCATGAACAAAATTACGTGACTTAAATTGCACAATGCAGCCCACTTGTTAGTTATCCATCTACAAAATTGAACATTTATATGTACTTAGTATGATGAAAACACGACTAGTTGATGGTATTACTTGCATTTTTTTGTTCTCTCCCAATTCTGCATCCATGGATTTTGGGGCTTTTACAAATTATTCATAGAATATCAAACTCAGCTTAACTCTTCAGCAGGGTAGGAATCAGTTCAATGATTGAATAATTTTGTTTTGAACATCATCGGTTTCACTTGCATCACATTGATCAATAGGTCAGAGTAGAACTACTATTAGTGAAATTGTTAAGGACATAGAAAATTCTCAATCCTAAAGAAATCAGGGGATTCTTTTCTAAATGTTAATAATATAGAAATTTTATTGCCTGCATAACTTCATATGAATCCTCTCAAAAAAAAAAAAAACTAGATATGGAGGTCTACCAGTAGTTGACTATTTGTGTAGCCGTTGTGCAATAGTTTACAAAATGCAATAGATTTAGTGTACTTATATTAGACAATATTTTTTTATAAGGATAGACAAATGTGAGTAGGTTACTGGTTATGTTAACACTTTCTGTTACTACAAACAAACAAAAGAAAGTAATCACACAAAAAGAAAAAAAAAAAAACACAAGAACGAAACAAACCCTCCTTATTACATATTCTTCTTCGCCACTCTCTCTCTCTCTCTCTCTCAAACTATCTCTCTGACTCTCGCTCTCTACAATCTGGATCACCATTCTTTGAATGGAACTTGTCGTTTTTGTTGTTCCAACGATAGGACATCAATCCTTGATCTACACCTTAGTCGCTTGCGGCAGTGTAATTTTGGCAGAATACAATGAATTCACCGGTAGCTTGACTAACATTCAGCAAGCAGTGCCTCCCTACGCAGTGAAAGTTAGTGAATACCAATAA

Protein sequence

>MS.gene38127.t1

MATIAKVTLLSSPLTFKTSFLPKSSSLSHTIASPKTTSLGIKVCAQLGGRDEETKKGEKKKFITREQEPEQYWQSAGEREGENPMMTPLPYIILFGHQSLIYTLVACGSVILAEYNEFTGSLTNIQQAVPPYAVKVSEYQ