Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76536.t1 | XP_003597685.1 | 95 | 202 | 7 | 1 | 1 | 202 | 1 | 199 | 9.80E-84 | 319.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76536.t1 | G7II07 | 95.0 | 202 | 7 | 1 | 1 | 202 | 1 | 199 | 7.1e-84 | 319.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene76536 | -0.80943 | 1.90E-50 | -1.69E-46 |
| MS.gene049171 | MS.gene76536 | -0.828897 | 6.96E-55 | -1.69E-46 |
| MS.gene049222 | MS.gene76536 | -0.830372 | 3.05E-55 | -1.69E-46 |
| MS.gene049285 | MS.gene76536 | -0.817408 | 3.35E-52 | -1.69E-46 |
| MS.gene049320 | MS.gene76536 | -0.827678 | 1.37E-54 | -1.69E-46 |
| MS.gene04932 | MS.gene76536 | 0.814185 | 1.75E-51 | -1.69E-46 |
| MS.gene04939 | MS.gene76536 | -0.805757 | 1.14E-49 | -1.69E-46 |
| MS.gene049549 | MS.gene76536 | 0.829348 | 5.41E-55 | -1.69E-46 |
| MS.gene049633 | MS.gene76536 | -0.811059 | 8.46E-51 | -1.69E-46 |
| MS.gene050163 | MS.gene76536 | -0.82679 | 2.24E-54 | -1.69E-46 |
| MS.gene050165 | MS.gene76536 | -0.830151 | 3.45E-55 | -1.69E-46 |
| MS.gene050170 | MS.gene76536 | -0.820388 | 7.04E-53 | -1.69E-46 |
| MS.gene050333 | MS.gene76536 | -0.813162 | 2.94E-51 | -1.69E-46 |
| MS.gene050338 | MS.gene76536 | 0.819264 | 1.27E-52 | -1.69E-46 |
| MS.gene050444 | MS.gene76536 | -0.84193 | 3.52E-58 | -1.69E-46 |
| MS.gene050504 | MS.gene76536 | -0.825189 | 5.37E-54 | -1.69E-46 |
| MS.gene050577 | MS.gene76536 | -0.839395 | 1.63E-57 | -1.69E-46 |
| MS.gene050612 | MS.gene76536 | -0.832372 | 9.82E-56 | -1.69E-46 |
| MS.gene050699 | MS.gene76536 | -0.820037 | 8.48E-53 | -1.69E-46 |
| MS.gene050747 | MS.gene76536 | -0.802985 | 4.33E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene76536 | -0.804501 | 2.10E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene76536 | -0.801453 | 8.94E-49 | -1.69E-46 |
| MS.gene050904 | MS.gene76536 | -0.819986 | 8.71E-53 | -1.69E-46 |
| MS.gene050950 | MS.gene76536 | -0.800607 | 1.33E-48 | -1.69E-46 |
| MS.gene051138 | MS.gene76536 | 0.833613 | 4.82E-56 | -1.69E-46 |
| MS.gene051317 | MS.gene76536 | -0.832814 | 7.62E-56 | -1.69E-46 |
| MS.gene051440 | MS.gene76536 | 0.802738 | 4.86E-49 | -1.69E-46 |
| MS.gene051517 | MS.gene76536 | 0.800915 | 1.15E-48 | -1.69E-46 |
| MS.gene051552 | MS.gene76536 | 0.80257 | 5.27E-49 | -1.69E-46 |
| MS.gene051651 | MS.gene76536 | 0.841867 | 3.66E-58 | -1.69E-46 |
| MS.gene051838 | MS.gene76536 | -0.803946 | 2.74E-49 | -1.69E-46 |
| MS.gene051973 | MS.gene76536 | 0.83398 | 3.90E-56 | -1.69E-46 |
| MS.gene052050 | MS.gene76536 | 0.808118 | 3.62E-50 | -1.69E-46 |
| MS.gene052164 | MS.gene76536 | -0.813064 | 3.09E-51 | -1.69E-46 |
| MS.gene052211 | MS.gene76536 | 0.842467 | 2.54E-58 | -1.69E-46 |
| MS.gene05240 | MS.gene76536 | 0.802179 | 6.34E-49 | -1.69E-46 |
| MS.gene05244 | MS.gene76536 | 0.805678 | 1.19E-49 | -1.69E-46 |
| MS.gene052482 | MS.gene76536 | -0.809811 | 1.57E-50 | -1.69E-46 |
| MS.gene052486 | MS.gene76536 | -0.815218 | 1.03E-51 | -1.69E-46 |
| MS.gene052494 | MS.gene76536 | 0.805516 | 1.29E-49 | -1.69E-46 |
| MS.gene052503 | MS.gene76536 | -0.805607 | 1.23E-49 | -1.69E-46 |
| MS.gene052606 | MS.gene76536 | 0.856334 | 3.45E-62 | -1.69E-46 |
| MS.gene052615 | MS.gene76536 | -0.804139 | 2.49E-49 | -1.69E-46 |
| MS.gene052823 | MS.gene76536 | -0.809643 | 1.71E-50 | -1.69E-46 |
| MS.gene052825 | MS.gene76536 | -0.805871 | 1.08E-49 | -1.69E-46 |
| MS.gene053203 | MS.gene76536 | -0.803775 | 2.97E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene76536 | 0.80354 | 3.32E-49 | -1.69E-46 |
| MS.gene053421 | MS.gene76536 | 0.804063 | 2.59E-49 | -1.69E-46 |
| MS.gene053479 | MS.gene76536 | -0.80982 | 1.57E-50 | -1.69E-46 |
| MS.gene053480 | MS.gene76536 | -0.812311 | 4.52E-51 | -1.69E-46 |
| MS.gene053579 | MS.gene76536 | -0.821961 | 3.06E-53 | -1.69E-46 |
| MS.gene053632 | MS.gene76536 | -0.801305 | 9.59E-49 | -1.69E-46 |
| MS.gene053776 | MS.gene76536 | 0.824263 | 8.88E-54 | -1.69E-46 |
| MS.gene053824 | MS.gene76536 | -0.806583 | 7.67E-50 | -1.69E-46 |
| MS.gene054107 | MS.gene76536 | -0.802831 | 4.66E-49 | -1.69E-46 |
| MS.gene054146 | MS.gene76536 | 0.814184 | 1.75E-51 | -1.69E-46 |
| MS.gene054408 | MS.gene76536 | -0.802909 | 4.49E-49 | -1.69E-46 |
| MS.gene054642 | MS.gene76536 | -0.802881 | 4.55E-49 | -1.69E-46 |
| MS.gene054706 | MS.gene76536 | 0.809214 | 2.11E-50 | -1.69E-46 |
| MS.gene05470 | MS.gene76536 | -0.8809 | 3.75E-70 | -1.69E-46 |
| MS.gene054884 | MS.gene76536 | 0.842286 | 2.84E-58 | -1.69E-46 |
| MS.gene054954 | MS.gene76536 | -0.809589 | 1.76E-50 | -1.69E-46 |
| MS.gene055154 | MS.gene76536 | 0.801945 | 7.09E-49 | -1.69E-46 |
| MS.gene055158 | MS.gene76536 | 0.820514 | 6.59E-53 | -1.69E-46 |
| MS.gene055240 | MS.gene76536 | 0.808113 | 3.63E-50 | -1.69E-46 |
| MS.gene055362 | MS.gene76536 | -0.817944 | 2.54E-52 | -1.69E-46 |
| MS.gene055502 | MS.gene76536 | -0.843925 | 1.04E-58 | -1.69E-46 |
| MS.gene055577 | MS.gene76536 | -0.813369 | 2.65E-51 | -1.69E-46 |
| MS.gene055729 | MS.gene76536 | 0.812492 | 4.13E-51 | -1.69E-46 |
| MS.gene055730 | MS.gene76536 | 0.811999 | 5.29E-51 | -1.69E-46 |
| MS.gene055731 | MS.gene76536 | 0.826678 | 2.38E-54 | -1.69E-46 |
| MS.gene055748 | MS.gene76536 | -0.851444 | 8.84E-61 | -1.69E-46 |
| MS.gene055750 | MS.gene76536 | -0.813531 | 2.44E-51 | -1.69E-46 |
| MS.gene055751 | MS.gene76536 | -0.847045 | 1.48E-59 | -1.69E-46 |
| MS.gene05589 | MS.gene76536 | -0.856835 | 2.46E-62 | -1.69E-46 |
| MS.gene056224 | MS.gene76536 | 0.805106 | 1.57E-49 | -1.69E-46 |
| MS.gene056387 | MS.gene76536 | -0.803494 | 3.39E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene76536 | -0.837885 | 3.99E-57 | -1.69E-46 |
| MS.gene056590 | MS.gene76536 | 0.834819 | 2.40E-56 | -1.69E-46 |
| MS.gene056591 | MS.gene76536 | 0.83529 | 1.83E-56 | -1.69E-46 |
| MS.gene056657 | MS.gene76536 | -0.821796 | 3.34E-53 | -1.69E-46 |
| MS.gene056678 | MS.gene76536 | 0.812261 | 4.63E-51 | -1.69E-46 |
| MS.gene056688 | MS.gene76536 | 0.816331 | 5.84E-52 | -1.69E-46 |
| MS.gene056692 | MS.gene76536 | -0.840904 | 6.57E-58 | -1.69E-46 |
| MS.gene056694 | MS.gene76536 | 0.852832 | 3.56E-61 | -1.69E-46 |
| MS.gene056697 | MS.gene76536 | 0.845542 | 3.81E-59 | -1.69E-46 |
| MS.gene056748 | MS.gene76536 | 0.826911 | 2.09E-54 | -1.69E-46 |
| MS.gene056856 | MS.gene76536 | 0.848796 | 4.88E-60 | -1.69E-46 |
| MS.gene056988 | MS.gene76536 | 0.818727 | 1.69E-52 | -1.69E-46 |
| MS.gene057048 | MS.gene76536 | -0.802876 | 4.56E-49 | -1.69E-46 |
| MS.gene057261 | MS.gene76536 | 0.816513 | 5.32E-52 | -1.69E-46 |
| MS.gene057658 | MS.gene76536 | -0.830139 | 3.48E-55 | -1.69E-46 |
| MS.gene057717 | MS.gene76536 | 0.831301 | 1.80E-55 | -1.69E-46 |
| MS.gene057768 | MS.gene76536 | 0.822789 | 1.96E-53 | -1.69E-46 |
| MS.gene057831 | MS.gene76536 | -0.824836 | 6.50E-54 | -1.69E-46 |
| MS.gene058068 | MS.gene76536 | 0.807112 | 5.93E-50 | -1.69E-46 |
| MS.gene058158 | MS.gene76536 | 0.800753 | 1.24E-48 | -1.69E-46 |
| MS.gene058334 | MS.gene76536 | 0.826054 | 3.35E-54 | -1.69E-46 |
| MS.gene05837 | MS.gene76536 | -0.811285 | 7.56E-51 | -1.69E-46 |
| MS.gene058382 | MS.gene76536 | 0.801762 | 7.73E-49 | -1.69E-46 |
| MS.gene058418 | MS.gene76536 | 0.816255 | 6.08E-52 | -1.69E-46 |
| MS.gene058419 | MS.gene76536 | -0.839075 | 1.97E-57 | -1.69E-46 |
| MS.gene058452 | MS.gene76536 | 0.823208 | 1.57E-53 | -1.69E-46 |
| MS.gene058544 | MS.gene76536 | -0.839293 | 1.73E-57 | -1.69E-46 |
| MS.gene058603 | MS.gene76536 | 0.846911 | 1.61E-59 | -1.69E-46 |
| MS.gene058838 | MS.gene76536 | -0.857035 | 2.15E-62 | -1.69E-46 |
| MS.gene059022 | MS.gene76536 | -0.810056 | 1.39E-50 | -1.69E-46 |
| MS.gene059023 | MS.gene76536 | -0.841009 | 6.16E-58 | -1.69E-46 |
| MS.gene05914 | MS.gene76536 | -0.864068 | 1.59E-64 | -1.69E-46 |
| MS.gene05926 | MS.gene76536 | 0.824577 | 7.48E-54 | -1.69E-46 |
| MS.gene05934 | MS.gene76536 | -0.806001 | 1.02E-49 | -1.69E-46 |
| MS.gene059526 | MS.gene76536 | 0.814317 | 1.64E-51 | -1.69E-46 |
| MS.gene05960 | MS.gene76536 | 0.828851 | 7.14E-55 | -1.69E-46 |
| MS.gene059677 | MS.gene76536 | -0.847936 | 8.43E-60 | -1.69E-46 |
| MS.gene059718 | MS.gene76536 | -0.811518 | 6.73E-51 | -1.69E-46 |
| MS.gene059756 | MS.gene76536 | -0.807272 | 5.48E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene76536 | -0.81648 | 5.41E-52 | -1.69E-46 |
| MS.gene059953 | MS.gene76536 | 0.813054 | 3.11E-51 | -1.69E-46 |
| MS.gene059955 | MS.gene76536 | 0.833304 | 5.76E-56 | -1.69E-46 |
| MS.gene060087 | MS.gene76536 | 0.814404 | 1.57E-51 | -1.69E-46 |
| MS.gene060405 | MS.gene76536 | 0.817617 | 3.01E-52 | -1.69E-46 |
| MS.gene060762 | MS.gene76536 | -0.861004 | 1.39E-63 | -1.69E-46 |
| MS.gene06077 | MS.gene76536 | -0.839039 | 2.01E-57 | -1.69E-46 |
| MS.gene06084 | MS.gene76536 | 0.826545 | 2.56E-54 | -1.69E-46 |
| MS.gene060982 | MS.gene76536 | -0.82351 | 1.33E-53 | -1.69E-46 |
| MS.gene061044 | MS.gene76536 | 0.846504 | 2.08E-59 | -1.69E-46 |
| MS.gene061150 | MS.gene76536 | -0.822931 | 1.82E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76536.t1 | MTR_2g101130 | 97.030 | 202 | 3 | 1 | 1 | 202 | 1 | 199 | 1.32e-146 | 405 |
| MS.gene76536.t1 | MTR_2g101120 | 90.148 | 203 | 12 | 3 | 1 | 203 | 1 | 195 | 2.84e-131 | 367 |
| MS.gene76536.t1 | MTR_8g014030 | 68.473 | 203 | 49 | 6 | 1 | 202 | 1 | 189 | 4.02e-96 | 278 |
| MS.gene76536.t1 | MTR_8g014010 | 64.356 | 202 | 53 | 7 | 1 | 200 | 1 | 185 | 3.80e-84 | 247 |
| MS.gene76536.t1 | MTR_8g014020 | 63.758 | 149 | 39 | 6 | 55 | 202 | 1 | 135 | 3.09e-58 | 180 |
| MS.gene76536.t1 | MTR_6g088525 | 42.365 | 203 | 77 | 11 | 7 | 202 | 10 | 179 | 1.98e-38 | 131 |
| MS.gene76536.t1 | MTR_6g088545 | 39.320 | 206 | 88 | 7 | 3 | 202 | 6 | 180 | 1.88e-37 | 129 |
| MS.gene76536.t1 | MTR_6g088565 | 38.350 | 206 | 90 | 7 | 3 | 202 | 6 | 180 | 9.11e-36 | 124 |
| MS.gene76536.t1 | MTR_6g088505 | 39.614 | 207 | 86 | 9 | 3 | 202 | 6 | 180 | 8.17e-35 | 122 |
| MS.gene76536.t1 | MTR_0016s0060 | 39.894 | 188 | 75 | 7 | 22 | 202 | 1 | 157 | 8.99e-30 | 108 |
| MS.gene76536.t1 | MTR_8g102660 | 36.816 | 201 | 88 | 11 | 7 | 202 | 9 | 175 | 1.51e-28 | 105 |
| MS.gene76536.t1 | MTR_0016s0050 | 39.888 | 178 | 68 | 8 | 32 | 202 | 1 | 146 | 2.42e-26 | 99.4 |
| MS.gene76536.t1 | MTR_7g084690 | 34.222 | 225 | 106 | 13 | 1 | 203 | 1 | 205 | 6.04e-21 | 86.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76536.t1 | AT1G52140 | 47.196 | 214 | 88 | 8 | 1 | 202 | 1 | 201 | 1.62e-55 | 175 |
| MS.gene76536.t1 | AT3G16330 | 42.661 | 218 | 87 | 9 | 1 | 202 | 1 | 196 | 7.94e-44 | 145 |
| MS.gene76536.t1 | AT4G29110 | 39.706 | 204 | 87 | 7 | 1 | 202 | 14 | 183 | 2.22e-31 | 113 |
Find 56 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCCCTTGATGCGGTAAAT+TGG | 0.283815 | 2.3:-2766565 | None:intergenic |
| TGCGGTGACGTTTCCCTATT+GGG | 0.299531 | 2.3:-2766625 | None:intergenic |
| TCTCCAGCACTGCCTGGATT+CGG | 0.302018 | 2.3:+2766782 | MS.gene76536:CDS |
| CTGCGGTGACGTTTCCCTAT+TGG | 0.308179 | 2.3:-2766626 | None:intergenic |
| CTCCAGCACTGCCTGGATTC+GGG | 0.322990 | 2.3:+2766783 | MS.gene76536:CDS |
| TTCTCATCATCTTCTCTTAG+AGG | 0.334226 | 2.3:-2766851 | None:intergenic |
| ATCAAGCTTGCTTCCTCAAT+TGG | 0.341340 | 2.3:-2766925 | None:intergenic |
| CCCTCAATTGCCTTACCATT+GGG | 0.354815 | 2.3:-2766811 | None:intergenic |
| CTGCTGAAGATTTCATAAAC+AGG | 0.373468 | 2.3:+2766894 | MS.gene76536:CDS |
| CGCCCGAATCCAGGCAGTGC+TGG | 0.394166 | 2.3:-2766785 | None:intergenic |
| ATCAAAGAGAGTGTGGAGTA+TGG | 0.418765 | 2.3:+2766358 | MS.gene76536:CDS |
| GGCGTTTGAGCATCATGTTT+AGG | 0.451131 | 2.3:-2766439 | None:intergenic |
| CGTGGCTGTGGTGGCTGTGA+TGG | 0.466817 | 2.3:-2766505 | None:intergenic |
| ACCCTCAATTGCCTTACCAT+TGG | 0.467623 | 2.3:-2766812 | None:intergenic |
| GTCAAAGGGCAAACTAATGA+TGG | 0.467955 | 2.3:+2766415 | MS.gene76536:CDS |
| GCCTTCTTCATGTTGAGAAA+AGG | 0.468789 | 2.3:+2766389 | MS.gene76536:CDS |
| TGAAGAAATTGTTGTGATTG+TGG | 0.472141 | 2.3:-2766655 | None:intergenic |
| AGGTGGAGCCGCCGTGGCTG+TGG | 0.473691 | 2.3:-2766517 | None:intergenic |
| GAAGGATGATCAAGTTGATA+AGG | 0.483265 | 2.3:+2766871 | MS.gene76536:CDS |
| CCCTTGATGCGGTAAATTGG+TGG | 0.485419 | 2.3:-2766562 | None:intergenic |
| GCCCAATGGTAAGGCAATTG+AGG | 0.485585 | 2.3:+2766810 | MS.gene76536:CDS |
| GAAGCATCTCCAGCACTGCC+TGG | 0.487263 | 2.3:+2766776 | MS.gene76536:CDS |
| GAGTGCAATGAAGGCCGTGC+TGG | 0.499574 | 2.3:+2766724 | MS.gene76536:CDS |
| CATCACAGCCACCACAGCCA+CGG | 0.499967 | 2.3:+2766506 | MS.gene76536:CDS |
| TCATCTTCTCTTAGAGGGAA+TGG | 0.502641 | 2.3:-2766845 | None:intergenic |
| CCCAATGGTAAGGCAATTGA+GGG | 0.507064 | 2.3:+2766811 | MS.gene76536:CDS |
| GTCGTGAGAGCGGCTTGAGG+TGG | 0.508313 | 2.3:-2766534 | None:intergenic |
| ATTGGGCTACGCCCGAATCC+AGG | 0.513644 | 2.3:-2766794 | None:intergenic |
| GGCTGTGGTGGCTGTGATGG+TGG | 0.525762 | 2.3:-2766502 | None:intergenic |
| TCTCATCATCTTCTCTTAGA+GGG | 0.530370 | 2.3:-2766850 | None:intergenic |
| CACACTCTCTTTGATATCAC+TGG | 0.533500 | 2.3:-2766350 | None:intergenic |
| TTGAGAAAAGGCATGTCAAA+GGG | 0.534014 | 2.3:+2766401 | MS.gene76536:CDS |
| GCCACCAATTTACCGCATCA+AGG | 0.534674 | 2.3:+2766561 | MS.gene76536:CDS |
| TGGAGCCGCCGTGGCTGTGG+TGG | 0.543982 | 2.3:-2766514 | None:intergenic |
| CGCTCATACGCCACCTACAC+AGG | 0.551605 | 2.3:+2766682 | MS.gene76536:CDS |
| GTTGAGAAAAGGCATGTCAA+AGG | 0.559027 | 2.3:+2766400 | MS.gene76536:CDS |
| GTGGAACATTAGATTGGCAA+TGG | 0.565737 | 2.3:-2766483 | None:intergenic |
| AAACGCCGAAGCAAGCTCGC+CGG | 0.566114 | 2.3:+2766455 | MS.gene76536:CDS |
| GTGATGGTGGAACATTAGAT+TGG | 0.566670 | 2.3:-2766489 | None:intergenic |
| TCTAAGAGAAGATGATGAGA+AGG | 0.566713 | 2.3:+2766853 | MS.gene76536:CDS |
| TCGGGCGTAGCCCAATGGTA+AGG | 0.575007 | 2.3:+2766801 | MS.gene76536:CDS |
| GGCGACAATGAGTGCAATGA+AGG | 0.584967 | 2.3:+2766715 | MS.gene76536:CDS |
| TGGATTCGGGCGTAGCCCAA+TGG | 0.585672 | 2.3:+2766796 | MS.gene76536:CDS |
| ACATCATCGTCCTGTGTAGG+TGG | 0.587034 | 2.3:-2766692 | None:intergenic |
| TCAAAGGGCAAACTAATGAT+GGG | 0.588410 | 2.3:+2766416 | MS.gene76536:CDS |
| CCACCAATTTACCGCATCAA+GGG | 0.590913 | 2.3:+2766562 | MS.gene76536:CDS |
| CAGTGATATCAAAGAGAGTG+TGG | 0.593721 | 2.3:+2766351 | MS.gene76536:CDS |
| GGCTTGAGGTGGAGCCGCCG+TGG | 0.620681 | 2.3:-2766523 | None:intergenic |
| GGTGGCGTGAGTCGTGAGAG+CGG | 0.626926 | 2.3:-2766544 | None:intergenic |
| TGTGATTGTGGTTGTTGCTG+CGG | 0.635520 | 2.3:-2766643 | None:intergenic |
| GCCACATCATCGTCCTGTGT+AGG | 0.639849 | 2.3:-2766695 | None:intergenic |
| ATTGTTAAGCATCTCCAGCA+CGG | 0.641871 | 2.3:-2766738 | None:intergenic |
| CACAGCCACCACAGCCACGG+CGG | 0.652559 | 2.3:+2766509 | MS.gene76536:CDS |
| ACCTACACAGGACGATGATG+TGG | 0.656484 | 2.3:+2766694 | MS.gene76536:CDS |
| TGAGTCGTGAGAGCGGCTTG+AGG | 0.683296 | 2.3:-2766537 | None:intergenic |
| GAATTCATACTCCCTTGATG+CGG | 0.696823 | 2.3:-2766573 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGAAGAAATTGTTGTGATTG+TGG | - | chr2.3:2766658-2766677 | None:intergenic | 30.0% | |
| CTGCTGAAGATTTCATAAAC+AGG | + | chr2.3:2766894-2766913 | MS.gene76536:CDS | 35.0% | |
| TCAAAGGGCAAACTAATGAT+GGG | + | chr2.3:2766416-2766435 | MS.gene76536:CDS | 35.0% | |
| TCTAAGAGAAGATGATGAGA+AGG | + | chr2.3:2766853-2766872 | MS.gene76536:CDS | 35.0% | |
| TCTCATCATCTTCTCTTAGA+GGG | - | chr2.3:2766853-2766872 | None:intergenic | 35.0% | |
| TTCTCATCATCTTCTCTTAG+AGG | - | chr2.3:2766854-2766873 | None:intergenic | 35.0% | |
| ! | TTGAGAAAAGGCATGTCAAA+GGG | + | chr2.3:2766401-2766420 | MS.gene76536:CDS | 35.0% |
| !! | GAAGGATGATCAAGTTGATA+AGG | + | chr2.3:2766871-2766890 | MS.gene76536:CDS | 35.0% |
| ATTGTTAAGCATCTCCAGCA+CGG | - | chr2.3:2766741-2766760 | None:intergenic | 40.0% | |
| CACACTCTCTTTGATATCAC+TGG | - | chr2.3:2766353-2766372 | None:intergenic | 40.0% | |
| CAGTGATATCAAAGAGAGTG+TGG | + | chr2.3:2766351-2766370 | MS.gene76536:CDS | 40.0% | |
| CCCTATTGGGAAAAAGTGAT+GGG | - | chr2.3:2766615-2766634 | None:intergenic | 40.0% | |
| CCTATTGGGAAAAAGTGATG+GGG | - | chr2.3:2766614-2766633 | None:intergenic | 40.0% | |
| GAATTCATACTCCCTTGATG+CGG | - | chr2.3:2766576-2766595 | None:intergenic | 40.0% | |
| GCCTTCTTCATGTTGAGAAA+AGG | + | chr2.3:2766389-2766408 | MS.gene76536:CDS | 40.0% | |
| GTCAAAGGGCAAACTAATGA+TGG | + | chr2.3:2766415-2766434 | MS.gene76536:CDS | 40.0% | |
| GTGGAACATTAGATTGGCAA+TGG | - | chr2.3:2766486-2766505 | None:intergenic | 40.0% | |
| TCATCTTCTCTTAGAGGGAA+TGG | - | chr2.3:2766848-2766867 | None:intergenic | 40.0% | |
| TCCCTATTGGGAAAAAGTGA+TGG | - | chr2.3:2766616-2766635 | None:intergenic | 40.0% | |
| ! | ACAGGTTTTACAGCCAATTG+AGG | + | chr2.3:2766912-2766931 | MS.gene76536:CDS | 40.0% |
| ! | ATCAAAGAGAGTGTGGAGTA+TGG | + | chr2.3:2766358-2766377 | MS.gene76536:CDS | 40.0% |
| ! | GCCTTTTCTCAACATGAAGA+AGG | - | chr2.3:2766393-2766412 | None:intergenic | 40.0% |
| ! | GTTGAGAAAAGGCATGTCAA+AGG | + | chr2.3:2766400-2766419 | MS.gene76536:CDS | 40.0% |
| ! | TAGATTGGCAATGGCTTTTC+CGG | - | chr2.3:2766477-2766496 | None:intergenic | 40.0% |
| !! | GTGATGGTGGAACATTAGAT+TGG | - | chr2.3:2766492-2766511 | None:intergenic | 40.0% |
| !!! | CCCATCACTTTTTCCCAATA+GGG | + | chr2.3:2766612-2766631 | MS.gene76536:CDS | 40.0% |
| ACCCTCAATTGCCTTACCAT+TGG | - | chr2.3:2766815-2766834 | None:intergenic | 45.0% | |
| ACTCCCTTGATGCGGTAAAT+TGG | - | chr2.3:2766568-2766587 | None:intergenic | 45.0% | |
| CCACCAATTTACCGCATCAA+GGG | + | chr2.3:2766562-2766581 | MS.gene76536:CDS | 45.0% | |
| CCCAATGGTAAGGCAATTGA+GGG | + | chr2.3:2766811-2766830 | MS.gene76536:CDS | 45.0% | |
| CCCTCAATTGCCTTACCATT+GGG | - | chr2.3:2766814-2766833 | None:intergenic | 45.0% | |
| GGCGTTTGAGCATCATGTTT+AGG | - | chr2.3:2766442-2766461 | None:intergenic | 45.0% | |
| TGTGATTGTGGTTGTTGCTG+CGG | - | chr2.3:2766646-2766665 | None:intergenic | 45.0% | |
| !!! | CCCCATCACTTTTTCCCAAT+AGG | + | chr2.3:2766611-2766630 | MS.gene76536:CDS | 45.0% |
| ACATCATCGTCCTGTGTAGG+TGG | - | chr2.3:2766695-2766714 | None:intergenic | 50.0% | |
| ACCTACACAGGACGATGATG+TGG | + | chr2.3:2766694-2766713 | MS.gene76536:CDS | 50.0% | |
| CCCTTGATGCGGTAAATTGG+TGG | - | chr2.3:2766565-2766584 | None:intergenic | 50.0% | |
| GCCACCAATTTACCGCATCA+AGG | + | chr2.3:2766561-2766580 | MS.gene76536:CDS | 50.0% | |
| GCCCAATGGTAAGGCAATTG+AGG | + | chr2.3:2766810-2766829 | MS.gene76536:CDS | 50.0% | |
| GGCGACAATGAGTGCAATGA+AGG | + | chr2.3:2766715-2766734 | MS.gene76536:CDS | 50.0% | |
| TGCGGTGACGTTTCCCTATT+GGG | - | chr2.3:2766628-2766647 | None:intergenic | 50.0% | |
| CTGCGGTGACGTTTCCCTAT+TGG | - | chr2.3:2766629-2766648 | None:intergenic | 55.0% | |
| GCCACATCATCGTCCTGTGT+AGG | - | chr2.3:2766698-2766717 | None:intergenic | 55.0% | |
| TCTCCAGCACTGCCTGGATT+CGG | + | chr2.3:2766782-2766801 | MS.gene76536:CDS | 55.0% | |
| !! | CTTTTCCGGCGAGCTTGCTT+CGG | - | chr2.3:2766463-2766482 | None:intergenic | 55.0% |
| AAACGCCGAAGCAAGCTCGC+CGG | + | chr2.3:2766455-2766474 | MS.gene76536:CDS | 60.0% | |
| ATTGGGCTACGCCCGAATCC+AGG | - | chr2.3:2766797-2766816 | None:intergenic | 60.0% | |
| CATCACAGCCACCACAGCCA+CGG | + | chr2.3:2766506-2766525 | MS.gene76536:CDS | 60.0% | |
| CGCTCATACGCCACCTACAC+AGG | + | chr2.3:2766682-2766701 | MS.gene76536:CDS | 60.0% | |
| CTCCAGCACTGCCTGGATTC+GGG | + | chr2.3:2766783-2766802 | MS.gene76536:CDS | 60.0% | |
| GAGTGCAATGAAGGCCGTGC+TGG | + | chr2.3:2766724-2766743 | MS.gene76536:CDS | 60.0% | |
| TCGGGCGTAGCCCAATGGTA+AGG | + | chr2.3:2766801-2766820 | MS.gene76536:CDS | 60.0% | |
| TGAGTCGTGAGAGCGGCTTG+AGG | - | chr2.3:2766540-2766559 | None:intergenic | 60.0% | |
| TGGATTCGGGCGTAGCCCAA+TGG | + | chr2.3:2766796-2766815 | MS.gene76536:CDS | 60.0% | |
| ! | GAAGCATCTCCAGCACTGCC+TGG | + | chr2.3:2766776-2766795 | MS.gene76536:CDS | 60.0% |
| CGTGGCTGTGGTGGCTGTGA+TGG | - | chr2.3:2766508-2766527 | None:intergenic | 65.0% | |
| GGCTGTGGTGGCTGTGATGG+TGG | - | chr2.3:2766505-2766524 | None:intergenic | 65.0% | |
| GGTGGCGTGAGTCGTGAGAG+CGG | - | chr2.3:2766547-2766566 | None:intergenic | 65.0% | |
| GTCGTGAGAGCGGCTTGAGG+TGG | - | chr2.3:2766537-2766556 | None:intergenic | 65.0% | |
| CACAGCCACCACAGCCACGG+CGG | + | chr2.3:2766509-2766528 | MS.gene76536:CDS | 70.0% | |
| CGCCCGAATCCAGGCAGTGC+TGG | - | chr2.3:2766788-2766807 | None:intergenic | 70.0% | |
| AGGTGGAGCCGCCGTGGCTG+TGG | - | chr2.3:2766520-2766539 | None:intergenic | 75.0% | |
| GGCTTGAGGTGGAGCCGCCG+TGG | - | chr2.3:2766526-2766545 | None:intergenic | 75.0% | |
| TGGAGCCGCCGTGGCTGTGG+TGG | - | chr2.3:2766517-2766536 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 2766335 | 2766946 | 2766335 | ID=MS.gene76536 |
| chr2.3 | mRNA | 2766335 | 2766946 | 2766335 | ID=MS.gene76536.t1;Parent=MS.gene76536 |
| chr2.3 | exon | 2766335 | 2766946 | 2766335 | ID=MS.gene76536.t1.exon1;Parent=MS.gene76536.t1 |
| chr2.3 | CDS | 2766335 | 2766946 | 2766335 | ID=cds.MS.gene76536.t1;Parent=MS.gene76536.t1 |
| Gene Sequence |
| Protein sequence |
