AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055565

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055565.t1 MTR_1g082760 98.976 293 3 0 1 293 1 293 0.0 607
MS.gene055565.t1 MTR_7g113650 86.301 292 40 0 1 292 1 292 0.0 544
MS.gene055565.t1 MTR_4g131740 71.088 294 84 1 1 293 1 294 1.53e-158 443
MS.gene055565.t1 MTR_6g023590 32.075 265 163 7 32 288 5 260 2.94e-31 117
MS.gene055565.t1 MTR_6g023610 33.962 265 148 8 39 290 12 262 4.17e-30 114
MS.gene055565.t1 MTR_6g023990 32.061 262 153 8 39 288 13 261 4.30e-30 114
MS.gene055565.t1 MTR_6g023600 33.962 265 158 7 32 288 5 260 1.12e-29 113
MS.gene055565.t1 MTR_6g023950 33.208 265 160 7 32 288 5 260 3.12e-29 112
MS.gene055565.t1 MTR_4g052320 34.231 260 155 7 39 292 7 256 3.58e-28 109
MS.gene055565.t1 MTR_3g085740 30.469 256 166 6 39 291 74 320 5.68e-28 110
MS.gene055565.t1 MTR_7g063270 32.031 256 158 7 40 288 16 262 1.11e-27 108
MS.gene055565.t1 MTR_4g052300 33.463 257 157 6 39 291 7 253 1.79e-27 107
MS.gene055565.t1 MTR_6g023910 32.836 268 149 10 35 287 8 259 6.76e-27 106
MS.gene055565.t1 MTR_4g132850 28.854 253 166 6 39 288 6 247 9.64e-26 102
MS.gene055565.t1 MTR_4g052350 32.685 257 159 6 39 291 13 259 1.20e-25 103
MS.gene055565.t1 MTR_3g020670 31.203 266 161 6 36 288 12 268 9.73e-25 100
MS.gene055565.t1 MTR_6g023600 33.208 265 154 8 32 288 5 254 9.74e-25 100
MS.gene055565.t1 MTR_7g063260 29.961 257 163 8 40 288 16 263 5.22e-24 98.6
MS.gene055565.t1 MTR_4g052340 30.233 258 166 5 39 292 13 260 5.45e-24 98.6
MS.gene055565.t1 MTR_4g078630 29.811 265 158 6 39 288 18 269 1.27e-23 97.8
MS.gene055565.t1 MTR_7g071680 27.957 279 158 9 39 288 28 292 2.40e-22 94.7
MS.gene055565.t1 MTR_8g442750 29.699 266 166 7 34 288 9 264 5.04e-22 94.0
MS.gene055565.t1 MTR_4g058810 26.766 269 179 6 35 293 6 266 4.72e-21 90.5
MS.gene055565.t1 MTR_7g105590 32.143 196 119 4 39 230 7 192 6.55e-21 88.6
MS.gene055565.t1 MTR_7g094510 30.385 260 162 8 40 290 6 255 1.99e-20 88.6
MS.gene055565.t1 MTR_4g058840 26.766 269 179 6 35 293 6 266 2.07e-20 89.0
MS.gene055565.t1 MTR_4g058820 26.766 269 179 6 35 293 6 266 2.07e-20 89.0
MS.gene055565.t1 MTR_4g064887 28.516 256 172 6 40 292 15 262 2.32e-20 88.6
MS.gene055565.t1 MTR_1196s0010 30.556 252 153 8 43 288 277 512 4.10e-20 90.5
MS.gene055565.t1 MTR_7g083870 29.278 263 162 7 39 288 34 285 5.73e-20 88.2
MS.gene055565.t1 MTR_7g063320 27.863 262 168 7 40 291 15 265 8.20e-19 84.3
MS.gene055565.t1 MTR_7g110140 28.897 263 158 6 39 288 5 251 1.90e-18 83.2
MS.gene055565.t1 MTR_2g090165 28.826 281 144 12 39 292 5 256 2.23e-18 83.2
MS.gene055565.t1 MTR_7g105460 35.176 199 117 4 39 234 12 201 3.00e-18 82.8
MS.gene055565.t1 MTR_7g063390 25.769 260 176 7 40 291 16 266 7.92e-18 81.6
MS.gene055565.t1 MTR_7g105580 29.741 232 128 6 37 264 5 205 9.89e-18 81.3
MS.gene055565.t1 MTR_7g094520 27.451 255 176 6 40 290 11 260 1.34e-17 80.9
MS.gene055565.t1 MTR_7g110130 27.341 267 167 8 39 292 5 257 2.39e-17 80.1
MS.gene055565.t1 MTR_4g064883 26.394 269 178 9 34 293 5 262 2.59e-17 80.1
MS.gene055565.t1 MTR_7g107670 26.136 264 168 7 39 288 32 282 1.23e-16 79.0
MS.gene055565.t1 MTR_8g096620 28.283 198 133 4 34 230 40 229 2.23e-16 78.6
MS.gene055565.t1 MTR_7g063270 26.772 254 125 6 40 288 16 213 1.05e-15 74.7
MS.gene055565.t1 MTR_1g068955 27.305 282 177 6 20 293 6 267 1.56e-15 75.5
MS.gene055565.t1 MTR_3g068065 27.027 259 174 8 40 293 17 265 2.16e-15 75.1
MS.gene055565.t1 MTR_6g035395 27.612 268 142 8 39 288 4 237 2.68e-15 74.3
MS.gene055565.t1 MTR_7g110160 28.470 281 127 13 39 292 5 238 4.96e-15 73.6
MS.gene055565.t1 MTR_3g068155 27.519 258 174 7 40 293 19 267 6.21e-15 73.6
MS.gene055565.t1 MTR_1g051165 26.238 202 143 4 33 228 7 208 7.78e-15 73.6
MS.gene055565.t1 MTR_8g096580 27.111 225 150 6 37 258 42 255 8.80e-15 73.9
MS.gene055565.t1 MTR_3g068175 26.357 258 177 6 40 293 17 265 1.50e-14 72.4
MS.gene055565.t1 MTR_3g087850 30.256 195 110 6 44 230 30 206 3.13e-14 72.0
MS.gene055565.t1 MTR_7g063360 25.188 266 170 8 40 291 16 266 3.82e-14 71.2
MS.gene055565.t1 MTR_5g008850 26.471 238 158 7 33 261 38 267 3.50e-13 69.3
MS.gene055565.t1 MTR_4g064887 27.411 197 134 4 40 234 15 204 2.00e-11 62.8
MS.gene055565.t1 MTR_4g084800 31.618 136 83 3 39 173 12 138 4.50e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055565.t1 AT1G54870 79.152 283 59 0 11 293 55 337 3.89e-174 484
MS.gene055565.t1 AT1G54870 79.152 283 59 0 11 293 53 335 6.19e-174 484
MS.gene055565.t1 AT3G05260 71.331 293 80 2 1 293 1 289 2.36e-163 456
MS.gene055565.t1 AT3G51680 28.664 307 179 10 8 288 1 293 1.39e-24 100
MS.gene055565.t1 AT4G05530 28.958 259 170 5 33 288 2 249 6.69e-24 98.2
MS.gene055565.t1 AT3G26770 30.189 265 161 7 37 288 38 291 3.35e-23 97.1
MS.gene055565.t1 AT1G24360 27.509 269 182 6 28 291 59 319 3.76e-23 97.4
MS.gene055565.t1 AT5G06060 31.395 258 164 7 40 293 9 257 1.55e-22 94.7
MS.gene055565.t1 AT2G47140 31.179 263 152 7 39 288 5 251 2.42e-22 94.0
MS.gene055565.t1 AT1G52340 30.451 266 160 8 39 288 17 273 2.62e-22 94.4
MS.gene055565.t1 AT1G63380 26.374 273 190 6 28 293 5 273 5.14e-22 93.6
MS.gene055565.t1 AT1G63380 26.692 266 184 6 35 293 14 275 7.50e-22 93.2
MS.gene055565.t1 AT1G63380 26.692 266 184 6 35 293 10 271 7.69e-22 92.8
MS.gene055565.t1 AT1G63380 26.692 266 184 6 35 293 17 278 8.36e-22 93.2
MS.gene055565.t1 AT1G62610 26.007 273 191 6 28 293 2 270 7.60e-21 90.1
MS.gene055565.t1 AT1G62610 26.316 266 185 6 35 293 10 271 8.90e-21 90.1
MS.gene055565.t1 AT1G62610 26.316 266 185 6 35 293 8 269 8.98e-21 90.1
MS.gene055565.t1 AT1G62610 26.316 266 185 6 35 293 14 275 9.77e-21 90.1
MS.gene055565.t1 AT2G29290 29.845 258 168 7 40 293 7 255 1.23e-20 89.4
MS.gene055565.t1 AT2G47120 30.798 263 159 7 38 291 5 253 2.33e-20 88.6
MS.gene055565.t1 AT2G29350 30.233 258 166 7 40 293 15 262 2.70e-20 88.6
MS.gene055565.t1 AT2G47120 30.916 262 158 7 39 291 5 252 2.89e-20 88.2
MS.gene055565.t1 AT3G04000 28.244 262 175 7 40 291 14 272 3.19e-20 88.6
MS.gene055565.t1 AT3G55290 29.000 200 133 4 34 228 12 207 2.06e-19 86.3
MS.gene055565.t1 AT3G55290 29.000 200 133 4 34 228 11 206 2.51e-19 85.9
MS.gene055565.t1 AT3G26760 28.731 268 163 10 37 288 33 288 4.10e-19 85.9
MS.gene055565.t1 AT2G47130 30.038 263 154 8 39 288 5 250 4.39e-19 85.1
MS.gene055565.t1 AT2G29150 29.057 265 170 8 35 293 11 263 4.88e-19 85.1
MS.gene055565.t1 AT2G29260 29.502 261 165 8 40 293 68 316 5.01e-19 85.9
MS.gene055565.t1 AT3G12800 28.044 271 165 10 34 288 5 261 7.78e-19 85.1
MS.gene055565.t1 AT5G50600 26.423 246 166 6 37 280 42 274 8.47e-19 85.5
MS.gene055565.t1 AT5G50700 26.423 246 166 6 37 280 42 274 8.47e-19 85.5
MS.gene055565.t1 AT3G29260 30.682 264 151 8 39 288 5 250 9.18e-19 84.0
MS.gene055565.t1 AT5G18210 28.736 261 166 10 38 287 6 257 3.46e-18 82.4
MS.gene055565.t1 AT3G42960 29.057 265 161 5 39 288 7 259 4.16e-18 82.4
MS.gene055565.t1 AT4G13180 29.213 267 174 9 32 291 5 263 6.24e-18 82.0
MS.gene055565.t1 AT3G03980 27.239 268 177 8 35 290 9 270 8.61e-18 81.6
MS.gene055565.t1 AT3G29250 28.137 263 160 6 39 288 5 251 1.45e-17 80.9
MS.gene055565.t1 AT2G29360 27.925 265 174 7 35 293 11 264 2.25e-17 80.5
MS.gene055565.t1 AT3G29250 28.137 263 160 6 39 288 41 287 2.96e-17 80.5
MS.gene055565.t1 AT2G30670 29.658 263 162 9 40 293 7 255 5.35e-17 79.3
MS.gene055565.t1 AT1G07440 27.586 261 169 7 40 293 12 259 6.13e-17 79.3
MS.gene055565.t1 AT3G29250 28.244 262 159 6 40 288 44 289 6.59e-17 79.7
MS.gene055565.t1 AT4G03140 26.592 267 163 6 39 288 77 327 1.04e-16 79.7
MS.gene055565.t1 AT5G10050 32.292 192 114 5 44 232 9 187 1.16e-16 78.6
MS.gene055565.t1 AT3G55310 25.641 273 191 7 28 293 5 272 1.21e-16 78.6
MS.gene055565.t1 AT5G18210 28.455 246 157 9 38 272 6 243 4.37e-16 77.0
MS.gene055565.t1 AT2G29310 29.588 267 155 11 40 292 7 254 1.35e-15 75.5
MS.gene055565.t1 AT3G47350 24.427 262 171 8 30 274 34 285 1.82e-15 75.5
MS.gene055565.t1 AT2G17845 26.540 211 145 5 39 242 46 253 2.06e-15 75.5
MS.gene055565.t1 AT2G29370 26.296 270 172 8 35 293 11 264 2.63e-15 74.7
MS.gene055565.t1 AT2G29350 32.240 183 117 5 115 293 45 224 2.97e-15 73.9
MS.gene055565.t1 AT1G07450 27.308 260 173 8 39 293 7 255 5.63e-15 73.6
MS.gene055565.t1 AT5G50690 28.788 198 131 5 35 230 40 229 5.99e-15 73.9
MS.gene055565.t1 AT5G50590 28.788 198 131 5 35 230 40 229 5.99e-15 73.9
MS.gene055565.t1 AT5G50690 30.151 199 127 7 35 230 40 229 6.12e-15 73.2
MS.gene055565.t1 AT5G50590 30.151 199 127 7 35 230 40 229 6.12e-15 73.2
MS.gene055565.t1 AT3G47350 24.681 235 163 6 30 260 34 258 1.16e-14 73.6
MS.gene055565.t1 AT2G29290 30.208 192 128 5 106 293 49 238 1.48e-14 72.0
MS.gene055565.t1 AT3G46170 27.136 199 136 5 35 228 21 215 2.53e-14 72.0
MS.gene055565.t1 AT5G65205 30.000 190 117 5 44 230 10 186 2.67e-14 72.0
MS.gene055565.t1 AT2G29320 27.757 263 168 9 40 293 13 262 1.22e-12 67.0
MS.gene055565.t1 AT4G10020 26.486 185 127 4 33 215 40 217 1.41e-12 67.8
MS.gene055565.t1 AT2G29340 28.295 258 172 7 40 293 7 255 2.13e-12 66.2
MS.gene055565.t1 AT2G29310 32.961 179 101 7 125 293 84 253 4.00e-12 65.5
MS.gene055565.t1 AT2G29300 27.444 266 165 11 40 293 7 256 2.93e-11 63.2
MS.gene055565.t1 AT2G29320 32.203 177 106 7 125 293 74 244 7.24e-11 61.6

Find 72 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAAAGATGCTGGTATTAA+TGG 0.214643 1.2:-60096371 None:intergenic
AGCTGGTAAGCAAGGGTATA+AGG 0.257323 1.2:+60096315 MS.gene055565:CDS
TATACTTGAAGTTTGTTTGA+TGG 0.274878 1.2:-60095086 None:intergenic
AATGGCTGTAGCAGCTGATT+TGG 0.283657 1.2:+60095825 MS.gene055565:CDS
GATGAGATTGTTAATGCTTA+TGG 0.332143 1.2:+60095877 MS.gene055565:CDS
TTGTTTGATGGCTTGTAGTC+AGG 0.340288 1.2:-60095074 None:intergenic
GAGGAAGAAACTGCTCAATT+TGG 0.343335 1.2:+60096395 MS.gene055565:CDS
ATGGCTGTAGCAGCTGATTT+GGG 0.347891 1.2:+60095826 MS.gene055565:CDS
TGCAACCGTAGCACCTTCTA+AGG 0.360573 1.2:-60095713 None:intergenic
AAAAGCCACAATTGCACCTT+TGG 0.391395 1.2:-60096273 None:intergenic
ACAGGAGGTGACTCTGGAAT+TGG 0.394290 1.2:+60095667 MS.gene055565:CDS
GCCTGGGAAGGAACATGTTA+TGG 0.396697 1.2:+60095028 MS.gene055565:CDS
GTTGCATTTACATATGTAAA+GGG 0.404353 1.2:+60095730 MS.gene055565:CDS
TGTTAACAAGAATATCAATA+TGG 0.406163 1.2:-60095900 None:intergenic
TAGGATGGAGCAACCTCAAT+AGG 0.420125 1.2:-60096449 None:intergenic
GAGTGGCACCTGGGCCCATA+TGG 0.425158 1.2:+60096345 MS.gene055565:CDS
TGGTATTAATGGTGTCCATA+TGG 0.436531 1.2:-60096360 None:intergenic
CAAAAGCAAGAGACACAGCC+TGG 0.437059 1.2:+60095011 MS.gene055565:CDS
AAAATAAGAGGAACATTGGT+TGG 0.452767 1.2:-60096486 None:intergenic
TTGTTTGTAAGAATGGCTTC+AGG 0.454658 1.2:+60094969 None:intergenic
AGGGTCAATGGAGTGGCACC+TGG 0.463348 1.2:+60096335 MS.gene055565:CDS
GGTAAGGTAGCTGTAATAAC+AGG 0.464066 1.2:+60095649 MS.gene055565:CDS
TCTTCCTCCTTAAAAGATGC+TGG 0.464676 1.2:-60096380 None:intergenic
GTAATAACAGGAGGTGACTC+TGG 0.469701 1.2:+60095661 MS.gene055565:CDS
AAATCAGCTGCTACAGCCAT+TGG 0.473059 1.2:-60095823 None:intergenic
GTTAATTACCATTGGGGTGA+AGG 0.476514 1.2:-60096520 None:intergenic
GGTTGCATTTACATATGTAA+AGG 0.478222 1.2:+60095729 MS.gene055565:CDS
GCTGGTAAGCAAGGGTATAA+GGG 0.491721 1.2:+60096316 MS.gene055565:CDS
AGGACACACTAGAAATGATA+AGG 0.503643 1.2:+60095773 MS.gene055565:CDS
TGCAAAGAGTGCTGATGCTA+AGG 0.507275 1.2:+60095798 MS.gene055565:CDS
AGCAAGGGTATAAGGGTCAA+TGG 0.508243 1.2:+60096323 MS.gene055565:CDS
TGTTTGATGGCTTGTAGTCA+GGG 0.510691 1.2:-60095073 None:intergenic
ATCACTTCAGCTGGTAAGCA+AGG 0.512052 1.2:+60096307 MS.gene055565:CDS
TCCATAACATGTTCCTTCCC+AGG 0.513835 1.2:-60095029 None:intergenic
GGTATTAATGGTGTCCATAT+GGG 0.514396 1.2:-60096359 None:intergenic
TCAGGTGGAACCGTGGTGAA+TGG 0.529354 1.2:+60096616 MS.gene055565:intron
GGGCAAGTAAATTGAGGCAG+TGG 0.536749 1.2:-60095053 None:intergenic
GAAGAAACTGCTCAATTTGG+TGG 0.540906 1.2:+60096398 MS.gene055565:CDS
GACTTGTCCAGTAAAATAAG+AGG 0.544263 1.2:-60096498 None:intergenic
GCACTGAAGCACATGAAGGA+AGG 0.556303 1.2:+60096179 MS.gene055565:CDS
TGCTGATGCTAAGGATCCAA+TGG 0.557285 1.2:+60095807 MS.gene055565:CDS
AATGGTGTCCATATGGGCCC+AGG 0.558819 1.2:-60096353 None:intergenic
GAGGCAAGAAAGACATAGGA+TGG 0.558839 1.2:-60096464 None:intergenic
AAGAGCTGGTCAGCCTATTG+AGG 0.560279 1.2:+60096436 MS.gene055565:CDS
ATCTACCAAAGGTGCAATTG+TGG 0.562333 1.2:+60096268 MS.gene055565:CDS
TGATGAAGACAAGGATGCTA+AGG 0.565763 1.2:+60095753 MS.gene055565:CDS
TGATGAGAATTGCAAGAGAG+TGG 0.569733 1.2:+60095852 MS.gene055565:CDS
CACTGAAGCACATGAAGGAA+GGG 0.573720 1.2:+60096180 MS.gene055565:CDS
AAGGGGACTATCACTTCAGC+TGG 0.574318 1.2:+60096298 MS.gene055565:CDS
CAAGTGCCTATGAAAAGAGC+TGG 0.576070 1.2:+60096422 MS.gene055565:CDS
AAAAGCAAGAGACACAGCCT+GGG 0.578653 1.2:+60095012 MS.gene055565:CDS
ATAAGAGGAACATTGGTTGG+AGG 0.579832 1.2:-60096483 None:intergenic
CAGTAAAATAAGAGGAACAT+TGG 0.583914 1.2:-60096490 None:intergenic
AAGTATGTTAATTACCATTG+GGG 0.584309 1.2:-60096526 None:intergenic
GCAAGAGACACAGCCTGGGA+AGG 0.594984 1.2:+60095016 MS.gene055565:CDS
GTTGGAGGCAAGAAAGACAT+AGG 0.599190 1.2:-60096468 None:intergenic
TTGAGGAGATTGATGAGTCA+AGG 0.599987 1.2:+60095953 MS.gene055565:CDS
TTGATGAGTCAAGGCTAGAG+AGG 0.603682 1.2:+60095962 MS.gene055565:CDS
TCTCATATTTCTTCATGACA+AGG 0.604054 1.2:+60096004 MS.gene055565:CDS
ATATGAGTGCTCATCAGTTG+AGG 0.605741 1.2:+60095936 MS.gene055565:CDS
TAGTCAGGGCAAGTAAATTG+AGG 0.610184 1.2:-60095059 None:intergenic
GGACAAGTCCTTCACCCCAA+TGG 0.617934 1.2:+60096512 MS.gene055565:CDS
TGATGAGTCAAGGCTAGAGA+GGG 0.619047 1.2:+60095963 MS.gene055565:CDS
TCACTTCAGCTGGTAAGCAA+GGG 0.625465 1.2:+60096308 MS.gene055565:CDS
GCATGCACTGAAGCACATGA+AGG 0.636267 1.2:+60096175 MS.gene055565:CDS
TGTAAAGGGTGATGAAGACA+AGG 0.640377 1.2:+60095744 MS.gene055565:CDS
GGGTATAAGGGTCAATGGAG+TGG 0.641256 1.2:+60096328 MS.gene055565:CDS
GGGTCAATGGAGTGGCACCT+GGG 0.643479 1.2:+60096336 MS.gene055565:CDS
TTAGACTATACATCTACCAA+AGG 0.664324 1.2:+60096257 MS.gene055565:CDS
AAGGTAGCTGTAATAACAGG+AGG 0.669413 1.2:+60095652 MS.gene055565:CDS
ACATCAGTGAATGCATACAA+AGG 0.684252 1.2:+60096221 MS.gene055565:CDS
GAGTCTTCAACCATTCACCA+CGG 0.723636 1.2:-60096626 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCTATTATTTACAATATT+TGG + chr1.2:60095404-60095423 MS.gene055565:intron 10.0%
!! TATAAGAAATAATTTGATAC+AGG - chr1.2:60095537-60095556 None:intergenic 15.0%
!! TGATGTTTACATTAATTAAT+TGG + chr1.2:60096562-60096581 MS.gene055565:intron 15.0%
!! TTCAATATAACTATATGAAT+TGG - chr1.2:60095150-60095169 None:intergenic 15.0%
!! TTGATTTAATATATATGACA+AGG + chr1.2:60095321-60095340 MS.gene055565:intron 15.0%
!!! AATATTTCTTTTTCAATTTG+AGG + chr1.2:60095250-60095269 MS.gene055565:intron 15.0%
!!! GTTATTTTTCTAAGTTATTA+TGG - chr1.2:60095515-60095534 None:intergenic 15.0%
!!! TTAAGATTAATATGTTTTTC+AGG + chr1.2:60096598-60096617 MS.gene055565:intron 15.0%
!!! TTTTATAATTTGGAACAAAA+TGG - chr1.2:60095176-60095195 None:intergenic 15.0%
!! ATATAACTATATGAATTGGT+TGG - chr1.2:60095146-60095165 None:intergenic 20.0%
!! CTCAAACTTACTATTAAAAT+AGG - chr1.2:60095355-60095374 None:intergenic 20.0%
!! TAAGTATGTTAATTACCATT+GGG - chr1.2:60096530-60096549 None:intergenic 20.0%
!! TGTTAACAAGAATATCAATA+TGG - chr1.2:60095903-60095922 None:intergenic 20.0%
!! TTAAGTATGTTAATTACCAT+TGG - chr1.2:60096531-60096550 None:intergenic 20.0%
! AAGTATGTTAATTACCATTG+GGG - chr1.2:60096529-60096548 None:intergenic 25.0%
! GTTGCATTTACATATGTAAA+GGG + chr1.2:60095730-60095749 MS.gene055565:CDS 25.0%
! TAAGAAACTTATGATATGTC+CGG + chr1.2:60095467-60095486 MS.gene055565:intron 25.0%
! TTAAAAGATGCTGGTATTAA+TGG - chr1.2:60096374-60096393 None:intergenic 25.0%
!! ATTAATACCAGCATCTTTTA+AGG + chr1.2:60096373-60096392 MS.gene055565:CDS 25.0%
!! TATACTTGAAGTTTGTTTGA+TGG - chr1.2:60095089-60095108 None:intergenic 25.0%
!! TTTTCTTGTGTTAAACTTCA+GGG + chr1.2:60095628-60095647 MS.gene055565:intron 25.0%
!! TTTTTCTTGTGTTAAACTTC+AGG + chr1.2:60095627-60095646 MS.gene055565:intron 25.0%
!!! AGATTAATATGTTTTTCAGG+TGG + chr1.2:60096601-60096620 MS.gene055565:intron 25.0%
!!! TGCAATTTATTTTCCTTAGA+AGG + chr1.2:60095700-60095719 MS.gene055565:CDS 25.0%
!!! TTTTTTATTAGAAGTTGTCC+CGG - chr1.2:60095489-60095508 None:intergenic 25.0%
AAAATAAGAGGAACATTGGT+TGG - chr1.2:60096489-60096508 None:intergenic 30.0%
AAGAAACTTATGATATGTCC+GGG + chr1.2:60095468-60095487 MS.gene055565:intron 30.0%
ATAAATCAACATCAACCCAA+TGG - chr1.2:60095383-60095402 None:intergenic 30.0%
CAGTAAAATAAGAGGAACAT+TGG - chr1.2:60096493-60096512 None:intergenic 30.0%
GATGAGATTGTTAATGCTTA+TGG + chr1.2:60095877-60095896 MS.gene055565:CDS 30.0%
GGTTGCATTTACATATGTAA+AGG + chr1.2:60095729-60095748 MS.gene055565:CDS 30.0%
TAAGTTTGAGTATCACCATT+GGG + chr1.2:60095365-60095384 MS.gene055565:intron 30.0%
TAATTGTACCATATATGTGC+AGG + chr1.2:60096153-60096172 MS.gene055565:intron 30.0%
TCTCATATTTCTTCATGACA+AGG + chr1.2:60096004-60096023 MS.gene055565:CDS 30.0%
TTAGACTATACATCTACCAA+AGG + chr1.2:60096257-60096276 MS.gene055565:CDS 30.0%
! AATTGGTTGGTATGGAAAAA+AGG - chr1.2:60095133-60095152 None:intergenic 30.0%
! ACTATATGAATTGGTTGGTA+TGG - chr1.2:60095141-60095160 None:intergenic 30.0%
!!! CAATGTTCCTCTTATTTTAC+TGG + chr1.2:60096491-60096510 MS.gene055565:CDS 30.0%
ACATCAGTGAATGCATACAA+AGG + chr1.2:60096221-60096240 MS.gene055565:CDS 35.0%
AGGACACACTAGAAATGATA+AGG + chr1.2:60095773-60095792 MS.gene055565:CDS 35.0%
GACTTGTCCAGTAAAATAAG+AGG - chr1.2:60096501-60096520 None:intergenic 35.0%
GGAAAAAAGGTGACAAATGA+AGG - chr1.2:60095120-60095139 None:intergenic 35.0%
GGTATTAATGGTGTCCATAT+GGG - chr1.2:60096362-60096381 None:intergenic 35.0%
GTAAGTTTGAGTATCACCAT+TGG + chr1.2:60095364-60095383 MS.gene055565:intron 35.0%
TCGAATATAACCACATGTGT+TGG - chr1.2:60096079-60096098 None:intergenic 35.0%
TGGTATTAATGGTGTCCATA+TGG - chr1.2:60096363-60096382 None:intergenic 35.0%
TTGTGTTAAACTTCAGGGTA+AGG + chr1.2:60095633-60095652 MS.gene055565:intron 35.0%
! TGCAATTGTGGCTTTTACAA+GGG + chr1.2:60096280-60096299 MS.gene055565:CDS 35.0%
!! AATACCAGCATCTTTTAAGG+AGG + chr1.2:60096376-60096395 MS.gene055565:CDS 35.0%
!!! ATTTTCCTTAGAAGGTGCTA+CGG + chr1.2:60095708-60095727 MS.gene055565:CDS 35.0%
AAAAGCCACAATTGCACCTT+TGG - chr1.2:60096276-60096295 None:intergenic 40.0%
AAGGTAGCTGTAATAACAGG+AGG + chr1.2:60095652-60095671 MS.gene055565:CDS 40.0%
ATATGAGTGCTCATCAGTTG+AGG + chr1.2:60095936-60095955 MS.gene055565:CDS 40.0%
CTACGACGTTGCTTATTCAT+CGG + chr1.2:60096113-60096132 MS.gene055565:intron 40.0%
GAAGAAACTGCTCAATTTGG+TGG + chr1.2:60096398-60096417 MS.gene055565:CDS 40.0%
GAGGAAGAAACTGCTCAATT+TGG + chr1.2:60096395-60096414 MS.gene055565:CDS 40.0%
GGTAAGGTAGCTGTAATAAC+AGG + chr1.2:60095649-60095668 MS.gene055565:CDS 40.0%
GTTAATTACCATTGGGGTGA+AGG - chr1.2:60096523-60096542 None:intergenic 40.0%
TAGTCAGGGCAAGTAAATTG+AGG - chr1.2:60095062-60095081 None:intergenic 40.0%
TCTTCCTCCTTAAAAGATGC+TGG - chr1.2:60096383-60096402 None:intergenic 40.0%
TGATGAAGACAAGGATGCTA+AGG + chr1.2:60095753-60095772 MS.gene055565:CDS 40.0%
TGATGAGAATTGCAAGAGAG+TGG + chr1.2:60095852-60095871 MS.gene055565:CDS 40.0%
TGTAAAGGGTGATGAAGACA+AGG + chr1.2:60095744-60095763 MS.gene055565:CDS 40.0%
TTGAGGAGATTGATGAGTCA+AGG + chr1.2:60095953-60095972 MS.gene055565:CDS 40.0%
! ATAAGAGGAACATTGGTTGG+AGG - chr1.2:60096486-60096505 None:intergenic 40.0%
! ATCTACCAAAGGTGCAATTG+TGG + chr1.2:60096268-60096287 MS.gene055565:CDS 40.0%
! GCAATTGTGGCTTTTACAAG+GGG + chr1.2:60096281-60096300 MS.gene055565:CDS 40.0%
! GTGCAATTGTGGCTTTTACA+AGG + chr1.2:60096279-60096298 MS.gene055565:CDS 40.0%
! TGTTTGATGGCTTGTAGTCA+GGG - chr1.2:60095076-60095095 None:intergenic 40.0%
! TTGTTTGATGGCTTGTAGTC+AGG - chr1.2:60095077-60095096 None:intergenic 40.0%
AAAAGCAAGAGACACAGCCT+GGG + chr1.2:60095012-60095031 MS.gene055565:CDS 45.0%
AAATCAGCTGCTACAGCCAT+TGG - chr1.2:60095826-60095845 None:intergenic 45.0%
AAGCAACGTCGTAGATGCTA+TGG - chr1.2:60096107-60096126 None:intergenic 45.0%
AATGGCTGTAGCAGCTGATT+TGG + chr1.2:60095825-60095844 MS.gene055565:CDS 45.0%
AGTGCATGCCTGCACATATA+TGG - chr1.2:60096164-60096183 None:intergenic 45.0%
ATCACTTCAGCTGGTAAGCA+AGG + chr1.2:60096307-60096326 MS.gene055565:CDS 45.0%
CAAGTGCCTATGAAAAGAGC+TGG + chr1.2:60096422-60096441 MS.gene055565:CDS 45.0%
CACTGAAGCACATGAAGGAA+GGG + chr1.2:60096180-60096199 MS.gene055565:CDS 45.0%
GAGGCAAGAAAGACATAGGA+TGG - chr1.2:60096467-60096486 None:intergenic 45.0%
GTAATAACAGGAGGTGACTC+TGG + chr1.2:60095661-60095680 MS.gene055565:CDS 45.0%
GTTGGAGGCAAGAAAGACAT+AGG - chr1.2:60096471-60096490 None:intergenic 45.0%
TAGGATGGAGCAACCTCAAT+AGG - chr1.2:60096452-60096471 None:intergenic 45.0%
TCACTTCAGCTGGTAAGCAA+GGG + chr1.2:60096308-60096327 MS.gene055565:CDS 45.0%
TCCATAACATGTTCCTTCCC+AGG - chr1.2:60095032-60095051 None:intergenic 45.0%
TGATGAGTCAAGGCTAGAGA+GGG + chr1.2:60095963-60095982 MS.gene055565:CDS 45.0%
TGCTGATGCTAAGGATCCAA+TGG + chr1.2:60095807-60095826 MS.gene055565:CDS 45.0%
TGTTACGTGTCCAACACATG+TGG + chr1.2:60096066-60096085 MS.gene055565:intron 45.0%
TTGATGAGTCAAGGCTAGAG+AGG + chr1.2:60095962-60095981 MS.gene055565:CDS 45.0%
! AGCAAGGGTATAAGGGTCAA+TGG + chr1.2:60096323-60096342 MS.gene055565:CDS 45.0%
! AGCTGGTAAGCAAGGGTATA+AGG + chr1.2:60096315-60096334 MS.gene055565:CDS 45.0%
! ATGGCTGTAGCAGCTGATTT+GGG + chr1.2:60095826-60095845 MS.gene055565:CDS 45.0%
! GCTGGTAAGCAAGGGTATAA+GGG + chr1.2:60096316-60096335 MS.gene055565:CDS 45.0%
!! CTGTGTCTCTTGCTTTTGTG+GGG - chr1.2:60095009-60095028 None:intergenic 45.0%
!! GCTGTGTCTCTTGCTTTTGT+GGG - chr1.2:60095010-60095029 None:intergenic 45.0%
!! TGCAAAGAGTGCTGATGCTA+AGG + chr1.2:60095798-60095817 MS.gene055565:CDS 45.0%
!! TGTGTCTCTTGCTTTTGTGG+GGG - chr1.2:60095008-60095027 None:intergenic 45.0%
!!! ATGTTTTTCAGGTGGAACCG+TGG + chr1.2:60096609-60096628 MS.gene055565:intron 45.0%
!!! ATTGTATAATTTTTATAATT+TGG - chr1.2:60095186-60095205 None:intergenic 5.0%
AAGAGCTGGTCAGCCTATTG+AGG + chr1.2:60096436-60096455 MS.gene055565:CDS 50.0%
AAGGGGACTATCACTTCAGC+TGG + chr1.2:60096298-60096317 MS.gene055565:CDS 50.0%
CAAAAGCAAGAGACACAGCC+TGG + chr1.2:60095011-60095030 MS.gene055565:CDS 50.0%
GCACTGAAGCACATGAAGGA+AGG + chr1.2:60096179-60096198 MS.gene055565:CDS 50.0%
GCATGCACTGAAGCACATGA+AGG + chr1.2:60096175-60096194 MS.gene055565:CDS 50.0%
GCCTGGGAAGGAACATGTTA+TGG + chr1.2:60095028-60095047 MS.gene055565:CDS 50.0%
GGGCAAGTAAATTGAGGCAG+TGG - chr1.2:60095056-60095075 None:intergenic 50.0%
GGGTATAAGGGTCAATGGAG+TGG + chr1.2:60096328-60096347 MS.gene055565:CDS 50.0%
TGCAACCGTAGCACCTTCTA+AGG - chr1.2:60095716-60095735 None:intergenic 50.0%
! GGCTGACCAGCTCTTTTCAT+AGG - chr1.2:60096431-60096450 None:intergenic 50.0%
!! ACAGGAGGTGACTCTGGAAT+TGG + chr1.2:60095667-60095686 MS.gene055565:CDS 50.0%
!! CAGAAGTGTCAGTGCTACAG+AGG - chr1.2:60096036-60096055 None:intergenic 50.0%
!! GGCTGTGTCTCTTGCTTTTG+TGG - chr1.2:60095011-60095030 None:intergenic 50.0%
!! GTGTCTCTTGCTTTTGTGGG+GGG - chr1.2:60095007-60095026 None:intergenic 50.0%
AATGGTGTCCATATGGGCCC+AGG - chr1.2:60096356-60096375 None:intergenic 55.0%
GGACAAGTCCTTCACCCCAA+TGG + chr1.2:60096512-60096531 MS.gene055565:CDS 55.0%
TCAGGTGGAACCGTGGTGAA+TGG + chr1.2:60096616-60096635 MS.gene055565:intron 55.0%
AGGGTCAATGGAGTGGCACC+TGG + chr1.2:60096335-60096354 MS.gene055565:CDS 60.0%
GCAAGAGACACAGCCTGGGA+AGG + chr1.2:60095016-60095035 MS.gene055565:CDS 60.0%
GGGTCAATGGAGTGGCACCT+GGG + chr1.2:60096336-60096355 MS.gene055565:CDS 60.0%
GAGTGGCACCTGGGCCCATA+TGG + chr1.2:60096345-60096364 MS.gene055565:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 60094981 60096642 60094981 ID=MS.gene055565
chr1.2 mRNA 60094981 60096642 60094981 ID=MS.gene055565.t1;Parent=MS.gene055565
chr1.2 exon 60094981 60095103 60094981 ID=MS.gene055565.t1.exon1;Parent=MS.gene055565.t1
chr1.2 CDS 60094981 60095103 60094981 ID=cds.MS.gene055565.t1;Parent=MS.gene055565.t1
chr1.2 exon 60095649 60096025 60095649 ID=MS.gene055565.t1.exon2;Parent=MS.gene055565.t1
chr1.2 CDS 60095649 60096025 60095649 ID=cds.MS.gene055565.t1;Parent=MS.gene055565.t1
chr1.2 exon 60096175 60096533 60096175 ID=MS.gene055565.t1.exon3;Parent=MS.gene055565.t1
chr1.2 CDS 60096175 60096533 60096175 ID=cds.MS.gene055565.t1;Parent=MS.gene055565.t1
chr1.2 exon 60096620 60096642 60096620 ID=MS.gene055565.t1.exon4;Parent=MS.gene055565.t1
chr1.2 CDS 60096620 60096642 60096620 ID=cds.MS.gene055565.t1;Parent=MS.gene055565.t1
Gene Sequence

>MS.gene055565

ATGGCTTCAGGTGAACAGAAATTCCCCCCACAAAAGCAAGAGACACAGCCTGGGAAGGAACATGTTATGGATCCACTGCCTCAATTTACTTGCCCTGACTACAAGCCATCAAACAAACTTCAAGTATATATATTCACCTTCATTTGTCACCTTTTTTCCATACCAACCAATTCATATAGTTATATTGAATCACCATTTTGTTCCAAATTATAAAAATTATACAATTGAATCATCTTGTTTACAAAATGTGAAAATCATTCTTAATTTAAAATATTTCTTTTTCAATTTGAGGATTTTATATTTGATGCTTATAATTTTGAGTATCAAAACAGTTATTTGTTTGATTTAATATATATGACAAGGTTCATAGACCTATTTTAATAGTAAGTTTGAGTATCACCATTGGGTTGATGTTGATTTATTTATCTATTATTTACAATATTTGGATTGATTTATTTGAGTTAAAATTATTTGAGAGTGTTTATATAAGAAACTTATGATATGTCCGGGACAACTTCTAATAAAAAAAATCCATAATAACTTAGAAAAATAACCTGTATCAAATTATTTCTTATATTTTTTACAAAAATAGTTTATGCATAAATACATGTACACAAACGATTAATTAATTTGTTTATACATGTTATTTTTCTTGTGTTAAACTTCAGGGTAAGGTAGCTGTAATAACAGGAGGTGACTCTGGAATTGGACGAGCAGTATGCAATTTATTTTCCTTAGAAGGTGCTACGGTTGCATTTACATATGTAAAGGGTGATGAAGACAAGGATGCTAAGGACACACTAGAAATGATAAGGAATGCAAAGAGTGCTGATGCTAAGGATCCAATGGCTGTAGCAGCTGATTTGGGTTTTGATGAGAATTGCAAGAGAGTGGTTGATGAGATTGTTAATGCTTATGGCCATATTGATATTCTTGTTAACAATGCTGCTGAGCAATATGAGTGCTCATCAGTTGAGGAGATTGATGAGTCAAGGCTAGAGAGGGTGTTCAGAACAAATATCTTCTCATATTTCTTCATGACAAGGTCAGTACCTCTGTAGCACTGACACTTCTGATAAAAGTTATGTTACGTGTCCAACACATGTGGTTATATTCGATTAATCCATAGCATCTACGACGTTGCTTATTCATCGGTAAACATCAAGTATATATAATTGTACCATATATGTGCAGGCATGCACTGAAGCACATGAAGGAAGGGAGCAGCATTATAAACACAACATCAGTGAATGCATACAAAGGAAATGCAAAGCTATTAGACTATACATCTACCAAAGGTGCAATTGTGGCTTTTACAAGGGGACTATCACTTCAGCTGGTAAGCAAGGGTATAAGGGTCAATGGAGTGGCACCTGGGCCCATATGGACACCATTAATACCAGCATCTTTTAAGGAGGAAGAAACTGCTCAATTTGGTGGTCAAGTGCCTATGAAAAGAGCTGGTCAGCCTATTGAGGTTGCTCCATCCTATGTCTTTCTTGCCTCCAACCAATGTTCCTCTTATTTTACTGGACAAGTCCTTCACCCCAATGGTAATTAACATACTTAAATTCTTGCTTCTGATGTTTACATTAATTAATTGGAGAAGTTTACTGATTAAGATTAATATGTTTTTCAGGTGGAACCGTGGTGAATGGTTGA
Protein sequence

>MS.gene055565.t1

MASGEQKFPPQKQETQPGKEHVMDPLPQFTCPDYKPSNKLQGKVAVITGGDSGIGRAVCNLFSLEGATVAFTYVKGDEDKDAKDTLEMIRNAKSADAKDPMAVAADLGFDENCKRVVDEIVNAYGHIDILVNNAAEQYECSSVEEIDESRLERVFRTNIFSYFFMTRHALKHMKEGSSIINTTSVNAYKGNAKLLDYTSTKGAIVAFTRGLSLQLVSKGIRVNGVAPGPIWTPLIPASFKEEETAQFGGQVPMKRAGQPIEVAPSYVFLASNQCSSYFTGQVLHPNGGTVVNG