AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071858


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071858.t1 MTR_2g435310 97.500 160 4 0 1 160 1 160 4.40e-114 320
MS.gene071858.t1 MTR_4g120760 74.843 159 40 0 1 159 1 159 9.87e-90 258
MS.gene071858.t1 MTR_4g120760 74.214 159 41 0 1 159 1 159 5.46e-89 256
MS.gene071858.t1 MTR_2g035100 41.026 156 91 1 1 156 1 155 2.67e-39 130
MS.gene071858.t1 MTR_2g035105 39.490 157 94 1 1 157 1 156 8.33e-38 127
MS.gene071858.t1 MTR_2g035150 38.854 157 95 1 1 157 1 156 9.08e-37 124
MS.gene071858.t1 MTR_2g035130 37.580 157 97 1 1 157 1 156 3.29e-36 123
MS.gene071858.t1 MTR_2g035120 37.580 157 97 1 1 157 1 156 6.02e-36 122
MS.gene071858.t1 MTR_6g033450 37.821 156 96 1 1 156 1 155 2.75e-33 115
MS.gene071858.t1 MTR_4g120970 37.821 156 96 1 1 156 1 155 2.75e-33 115
MS.gene071858.t1 MTR_2g035320 32.051 156 105 1 1 156 1 155 1.14e-29 106
MS.gene071858.t1 MTR_2g035170 36.184 152 94 2 7 156 5 155 2.57e-29 105
MS.gene071858.t1 MTR_2g035210 31.410 156 106 1 1 156 1 155 6.11e-29 104
MS.gene071858.t1 MTR_2g035220 31.126 151 103 1 6 156 6 155 9.62e-29 103
MS.gene071858.t1 MTR_2g035190 30.769 156 107 1 1 156 1 155 1.14e-27 101
MS.gene071858.t1 MTR_4g120950 34.177 158 100 3 1 156 1 156 7.51e-26 96.7
MS.gene071858.t1 MTR_2g035320 32.222 90 61 0 67 156 31 120 4.85e-13 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAACAACCTCCATCATTAA+TGG 0.267686 2.2:+53496144 None:intergenic
ACTAGCAAATACAATGCTAT+TGG 0.309101 2.2:-53496112 MS.gene071858:CDS
CTAGCAAATACAATGCTATT+GGG 0.326067 2.2:-53496111 MS.gene071858:CDS
ATGAACTGAGGTAAGAGTTT+TGG 0.327571 2.2:+53496829 None:intergenic
GCTTGAGTCAATTGCTTATG+AGG 0.335592 2.2:-53496179 MS.gene071858:CDS
TTCCAGCTTCTCCATTTCCT+TGG 0.338136 2.2:+53496788 None:intergenic
AGTCAAAGAGGAAGAGATAA+AGG 0.347021 2.2:-53496080 MS.gene071858:CDS
GACTCAAGCTTGTCACCTAA+TGG 0.355452 2.2:+53496193 None:intergenic
ACATACGTGATGGAGCTATA+GGG 0.397093 2.2:+53496887 None:intergenic
AACATACGTGATGGAGCTAT+AGG 0.429571 2.2:+53496886 None:intergenic
AGGAAGAGATAAAGGAAGGA+AGG 0.431527 2.2:-53496072 MS.gene071858:CDS
AGCTCCATCACGTATGTTCA+AGG 0.432574 2.2:-53496881 MS.gene071858:CDS
GTCAACGTAATCCAAGGAAA+TGG 0.442764 2.2:-53496799 MS.gene071858:CDS
TGCTTATGAGGTTAAGTTTG+AGG 0.448209 2.2:-53496167 MS.gene071858:CDS
CACAACTTTGCAAATTCCAA+TGG 0.469640 2.2:+53496042 None:intergenic
AAGTTTGAGGCCATTAATGA+TGG 0.470153 2.2:-53496154 MS.gene071858:CDS
GGAAGAATAATTATTTGTAA+AGG 0.473368 2.2:+53496909 None:intergenic
AGTTGTGGAAGCATACTTGC+TGG 0.476134 2.2:-53496026 MS.gene071858:CDS
ATCCAAGGAAATGGAGAAGC+TGG 0.480647 2.2:-53496790 MS.gene071858:CDS
ATGATTGAAGGAGATCCATT+AGG 0.495029 2.2:-53496208 MS.gene071858:CDS
CATTGGAATTTGCAAAGTTG+TGG 0.510944 2.2:-53496041 MS.gene071858:CDS
GGAAGAGATAAAGGAAGGAA+GGG 0.516313 2.2:-53496071 MS.gene071858:CDS
TGCAACTACACTATGATTGA+AGG 0.519866 2.2:-53496220 MS.gene071858:CDS
GAAGGAAGGGAAAACTCCAT+TGG 0.523267 2.2:-53496058 MS.gene071858:CDS
AAAGAGGAAGAGATAAAGGA+AGG 0.532049 2.2:-53496076 MS.gene071858:CDS
TATGATTGATAATGATAACT+TGG 0.540241 2.2:-53496245 MS.gene071858:CDS
CATACGTGATGGAGCTATAG+GGG 0.547471 2.2:+53496888 None:intergenic
TTTGAGGCCATTAATGATGG+AGG 0.588869 2.2:-53496151 MS.gene071858:CDS
AAAGATGTCAACGTAATCCA+AGG 0.591971 2.2:-53496805 MS.gene071858:CDS
TAGCAAATACAATGCTATTG+GGG 0.598363 2.2:-53496110 MS.gene071858:CDS
AATAACTAAACATAGACTTG+TGG 0.600652 2.2:+53495998 None:intergenic
AAAGCCTTGAACATACGTGA+TGG 0.622247 2.2:+53496877 None:intergenic
TGTAAAGGTTGAGACGACCA+TGG 0.669945 2.2:+53496924 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATGAATGATAAAATTTAA+AGG - chr2.2:53496435-53496454 MS.gene071858:intron 15.0%
!! ACCTCAAATAAAAAATATTT+AGG - chr2.2:53496405-53496424 MS.gene071858:intron 15.0%
!!! AAAAAAAAACATTTTAAAGC+TGG - chr2.2:53496199-53496218 MS.gene071858:CDS 15.0%
!!! TTTTCTTTTTTTCTTTATGT+TGG - chr2.2:53496270-53496289 MS.gene071858:CDS 15.0%
!! AACCCTAAATAAGATTTATT+TGG + chr2.2:53496553-53496572 None:intergenic 20.0%
!! ACCCTAAATAAGATTTATTT+GGG + chr2.2:53496552-53496571 None:intergenic 20.0%
!! CTTTCAAATATTTGAAAAAC+AGG - chr2.2:53496652-53496671 MS.gene071858:intron 20.0%
!! GGAAGAATAATTATTTGTAA+AGG + chr2.2:53496019-53496038 None:intergenic 20.0%
!!! ATAAGTAAGAATTGTTATTG+AGG + chr2.2:53496605-53496624 None:intergenic 20.0%
!!! CTGTTTTTCAAATATTTGAA+AGG + chr2.2:53496654-53496673 None:intergenic 20.0%
!!! GCCTAAATATTTTTTATTTG+AGG + chr2.2:53496409-53496428 None:intergenic 20.0%
!!! TATGATTGATAATGATAACT+TGG - chr2.2:53496680-53496699 MS.gene071858:intron 20.0%
!! GCCCAAATAAATCTTATTTA+GGG - chr2.2:53496548-53496567 MS.gene071858:intron 25.0%
!! TGCCCAAATAAATCTTATTT+AGG - chr2.2:53496547-53496566 MS.gene071858:intron 25.0%
AAAACAACCTCCATCATTAA+TGG + chr2.2:53496784-53496803 None:intergenic 30.0%
ACTAGCAAATACAATGCTAT+TGG - chr2.2:53496813-53496832 MS.gene071858:CDS 30.0%
CTAGCAAATACAATGCTATT+GGG - chr2.2:53496814-53496833 MS.gene071858:CDS 30.0%
TAGCAAATACAATGCTATTG+GGG - chr2.2:53496815-53496834 MS.gene071858:CDS 30.0%
! AATTGTTATTGAGGCAACAT+AGG + chr2.2:53496596-53496615 None:intergenic 30.0%
! GAACAAGTTAATTTTGCTGA+AGG - chr2.2:53496165-53496184 MS.gene071858:CDS 30.0%
! TTGACATCTTTTATGAACTG+AGG + chr2.2:53496111-53496130 None:intergenic 30.0%
AAAGAGGAAGAGATAAAGGA+AGG - chr2.2:53496849-53496868 MS.gene071858:CDS 35.0%
AAAGATGTCAACGTAATCCA+AGG - chr2.2:53496120-53496139 MS.gene071858:CDS 35.0%
AGTCAAAGAGGAAGAGATAA+AGG - chr2.2:53496845-53496864 MS.gene071858:CDS 35.0%
ATGATTGAAGGAGATCCATT+AGG - chr2.2:53496717-53496736 MS.gene071858:intron 35.0%
CACAACTTTGCAAATTCCAA+TGG + chr2.2:53496886-53496905 None:intergenic 35.0%
CATTGGAATTTGCAAAGTTG+TGG - chr2.2:53496884-53496903 MS.gene071858:CDS 35.0%
GTTGCGAGAAATACTTAGTT+TGG - chr2.2:53496378-53496397 MS.gene071858:intron 35.0%
TGCAACTACACTATGATTGA+AGG - chr2.2:53496705-53496724 MS.gene071858:intron 35.0%
TGCTTATGAGGTTAAGTTTG+AGG - chr2.2:53496758-53496777 MS.gene071858:intron 35.0%
! AAGTTTGAGGCCATTAATGA+TGG - chr2.2:53496771-53496790 MS.gene071858:CDS 35.0%
! ATGAACTGAGGTAAGAGTTT+TGG + chr2.2:53496099-53496118 None:intergenic 35.0%
AAAGCCTTGAACATACGTGA+TGG + chr2.2:53496051-53496070 None:intergenic 40.0%
AGGAAGAGATAAAGGAAGGA+AGG - chr2.2:53496853-53496872 MS.gene071858:CDS 40.0%
GCTTGAGTCAATTGCTTATG+AGG - chr2.2:53496746-53496765 MS.gene071858:intron 40.0%
GGAAGAGATAAAGGAAGGAA+GGG - chr2.2:53496854-53496873 MS.gene071858:CDS 40.0%
GTCAACGTAATCCAAGGAAA+TGG - chr2.2:53496126-53496145 MS.gene071858:CDS 40.0%
! AACATACGTGATGGAGCTAT+AGG + chr2.2:53496042-53496061 None:intergenic 40.0%
! ACATACGTGATGGAGCTATA+GGG + chr2.2:53496041-53496060 None:intergenic 40.0%
! TTTGAGGCCATTAATGATGG+AGG - chr2.2:53496774-53496793 MS.gene071858:CDS 40.0%
!!! TGGGGATTTTGAAGTCAAAG+AGG - chr2.2:53496833-53496852 MS.gene071858:CDS 40.0%
AGCTCCATCACGTATGTTCA+AGG - chr2.2:53496044-53496063 MS.gene071858:CDS 45.0%
ATCCAAGGAAATGGAGAAGC+TGG - chr2.2:53496135-53496154 MS.gene071858:CDS 45.0%
GAAGGAAGGGAAAACTCCAT+TGG - chr2.2:53496867-53496886 MS.gene071858:CDS 45.0%
GACTCAAGCTTGTCACCTAA+TGG + chr2.2:53496735-53496754 None:intergenic 45.0%
TTCCAGCTTCTCCATTTCCT+TGG + chr2.2:53496140-53496159 None:intergenic 45.0%
! AGTTGTGGAAGCATACTTGC+TGG - chr2.2:53496899-53496918 MS.gene071858:CDS 45.0%
! CATACGTGATGGAGCTATAG+GGG + chr2.2:53496040-53496059 None:intergenic 45.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 53496003 53496944 53496003 ID=MS.gene071858
chr2.2 mRNA 53496003 53496944 53496003 ID=MS.gene071858.t1;Parent=MS.gene071858
chr2.2 exon 53496761 53496944 53496761 ID=MS.gene071858.t1.exon1;Parent=MS.gene071858.t1
chr2.2 CDS 53496761 53496944 53496761 ID=cds.MS.gene071858.t1;Parent=MS.gene071858.t1
chr2.2 exon 53496003 53496301 53496003 ID=MS.gene071858.t1.exon2;Parent=MS.gene071858.t1
chr2.2 CDS 53496003 53496301 53496003 ID=cds.MS.gene071858.t1;Parent=MS.gene071858.t1
Gene Sequence

>MS.gene071858

ATGGTCGTCTCAACCTTTACAAATAATTATTCTTCCCCTATAGCTCCATCACGTATGTTCAAGGCTTTAATCACAGACTCTAGCAATTTGCTTCCAAAACTCTTACCTCAGTTCATAAAAGATGTCAACGTAATCCAAGGAAATGGAGAAGCTGGAACAATTGAACAAGTTAATTTTGCTGAAGGTTAGCAAAAAAAAAAAAAAACATTTTAAAGCTGGAAGAATTAAAATGCTCTTTTTATTTTTTATTTTTAAATTTAAAGAGATTTTTCTTTTTTTCTTTATGTTGGTTGAATAACTTCTTACTTACATGTTTCATTTTAGAGAGAAAAAATAGACTTATATTTTTATTGAAAAGTGATTTTTTTTTTCTAAGTTGCGAGAAATACTTAGTTTGGCACAACCTCAAATAAAAAATATTTAGGCACATACACATGAATGATAAAATTTAAAGGAAAAATAATTTAGTCACATTGCTCTCAAAAAAAAAAAAAAATTAGTCACATCATTTCTCGTTTAAATTTTCATCAATCATGTGTTTGTGTGCCCAAATAAATCTTATTTAGGGTTGTGTCAATACAAGACTTGCTCCTATGTTGCCTCAATAACAATTCTTACTTATATGTTCTATTTATCATTGCAGCTAGTCCTTTCAAATATTTGAAAAACAGGATTGATATGATTGATAATGATAACTTGGTGTGCAACTACACTATGATTGAAGGAGATCCATTAGGTGACAAGCTTGAGTCAATTGCTTATGAGGTTAAGTTTGAGGCCATTAATGATGGAGGTTGTTTTTGTAAAATGACTAGCAAATACAATGCTATTGGGGATTTTGAAGTCAAAGAGGAAGAGATAAAGGAAGGAAGGGAAAACTCCATTGGAATTTGCAAAGTTGTGGAAGCATACTTGCTGGAGAATCCACAAGTCTATGTTTAG

Protein sequence

>MS.gene071858.t1

MVVSTFTNNYSSPIAPSRMFKALITDSSNLLPKLLPQFIKDVNVIQGNGEAGTIEQVNFAEASPFKYLKNRIDMIDNDNLVCNYTMIEGDPLGDKLESIAYEVKFEAINDGGCFCKMTSKYNAIGDFEVKEEEIKEGRENSIGICKVVEAYLLENPQVYV