Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53321.t1 | XP_013444432.1 | 92.6 | 353 | 15 | 3 | 1 | 347 | 58 | 405 | 4.00E-101 | 378.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53321.t1 | Q9SLL2 | 52.1 | 211 | 95 | 3 | 132 | 341 | 159 | 364 | 3.8e-36 | 153.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53321.t1 | A0A072TM26 | 92.6 | 353 | 15 | 3 | 1 | 347 | 58 | 405 | 2.9e-101 | 378.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene53321 | 0.871175 | 8.35E-67 | -1.69E-46 |
| MS.gene049159 | MS.gene53321 | 0.842099 | 3.18E-58 | -1.69E-46 |
| MS.gene049254 | MS.gene53321 | 0.849236 | 3.68E-60 | -1.69E-46 |
| MS.gene049272 | MS.gene53321 | 0.808419 | 3.13E-50 | -1.69E-46 |
| MS.gene049343 | MS.gene53321 | 0.805712 | 1.17E-49 | -1.69E-46 |
| MS.gene049350 | MS.gene53321 | 0.839559 | 1.47E-57 | -1.69E-46 |
| MS.gene049368 | MS.gene53321 | 0.832174 | 1.10E-55 | -1.69E-46 |
| MS.gene049404 | MS.gene53321 | 0.816392 | 5.66E-52 | -1.69E-46 |
| MS.gene049501 | MS.gene53321 | 0.839443 | 1.58E-57 | -1.69E-46 |
| MS.gene049584 | MS.gene53321 | 0.808363 | 3.21E-50 | -1.69E-46 |
| MS.gene049585 | MS.gene53321 | 0.804715 | 1.89E-49 | -1.69E-46 |
| MS.gene049647 | MS.gene53321 | 0.806017 | 1.01E-49 | -1.69E-46 |
| MS.gene049711 | MS.gene53321 | 0.854032 | 1.61E-61 | -1.69E-46 |
| MS.gene049825 | MS.gene53321 | 0.804758 | 1.85E-49 | -1.69E-46 |
| MS.gene049850 | MS.gene53321 | 0.843404 | 1.43E-58 | -1.69E-46 |
| MS.gene050026 | MS.gene53321 | 0.80184 | 7.45E-49 | -1.69E-46 |
| MS.gene050137 | MS.gene53321 | 0.835404 | 1.71E-56 | -1.69E-46 |
| MS.gene050156 | MS.gene53321 | 0.849201 | 3.76E-60 | -1.69E-46 |
| MS.gene050251 | MS.gene53321 | 0.813414 | 2.59E-51 | -1.69E-46 |
| MS.gene050273 | MS.gene53321 | 0.803924 | 2.76E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene53321 | 0.805039 | 1.62E-49 | -1.69E-46 |
| MS.gene05045 | MS.gene53321 | 0.805223 | 1.48E-49 | -1.69E-46 |
| MS.gene050559 | MS.gene53321 | 0.817028 | 4.08E-52 | -1.69E-46 |
| MS.gene050561 | MS.gene53321 | 0.824191 | 9.23E-54 | -1.69E-46 |
| MS.gene050562 | MS.gene53321 | 0.808458 | 3.07E-50 | -1.69E-46 |
| MS.gene050657 | MS.gene53321 | 0.8196 | 1.07E-52 | -1.69E-46 |
| MS.gene050680 | MS.gene53321 | 0.801296 | 9.63E-49 | -1.69E-46 |
| MS.gene050759 | MS.gene53321 | 0.800446 | 1.44E-48 | -1.69E-46 |
| MS.gene050807 | MS.gene53321 | 0.831028 | 2.11E-55 | -1.69E-46 |
| MS.gene050826 | MS.gene53321 | 0.825459 | 4.63E-54 | -1.69E-46 |
| MS.gene050844 | MS.gene53321 | 0.842209 | 2.97E-58 | -1.69E-46 |
| MS.gene051180 | MS.gene53321 | 0.821487 | 3.93E-53 | -1.69E-46 |
| MS.gene051190 | MS.gene53321 | 0.805956 | 1.04E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene53321 | 0.812158 | 4.88E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene53321 | 0.867181 | 1.65E-65 | -1.69E-46 |
| MS.gene051265 | MS.gene53321 | 0.822205 | 2.68E-53 | -1.69E-46 |
| MS.gene051279 | MS.gene53321 | 0.833882 | 4.13E-56 | -1.69E-46 |
| MS.gene051332 | MS.gene53321 | 0.807783 | 4.27E-50 | -1.69E-46 |
| MS.gene051388 | MS.gene53321 | 0.805054 | 1.61E-49 | -1.69E-46 |
| MS.gene051490 | MS.gene53321 | 0.800923 | 1.15E-48 | -1.69E-46 |
| MS.gene05157 | MS.gene53321 | 0.807608 | 4.65E-50 | -1.69E-46 |
| MS.gene051594 | MS.gene53321 | 0.824076 | 9.82E-54 | -1.69E-46 |
| MS.gene051700 | MS.gene53321 | 0.829015 | 6.52E-55 | -1.69E-46 |
| MS.gene051719 | MS.gene53321 | 0.8718 | 5.18E-67 | -1.69E-46 |
| MS.gene051720 | MS.gene53321 | 0.866376 | 2.98E-65 | -1.69E-46 |
| MS.gene051744 | MS.gene53321 | 0.848895 | 4.58E-60 | -1.69E-46 |
| MS.gene051800 | MS.gene53321 | 0.824576 | 7.49E-54 | -1.69E-46 |
| MS.gene051957 | MS.gene53321 | 0.832242 | 1.06E-55 | -1.69E-46 |
| MS.gene052000 | MS.gene53321 | 0.803218 | 3.87E-49 | -1.69E-46 |
| MS.gene052012 | MS.gene53321 | 0.815536 | 8.79E-52 | -1.69E-46 |
| MS.gene052020 | MS.gene53321 | 0.827264 | 1.72E-54 | -1.69E-46 |
| MS.gene052042 | MS.gene53321 | 0.834916 | 2.27E-56 | -1.69E-46 |
| MS.gene052190 | MS.gene53321 | 0.81195 | 5.42E-51 | -1.69E-46 |
| MS.gene052323 | MS.gene53321 | 0.807908 | 4.02E-50 | -1.69E-46 |
| MS.gene052333 | MS.gene53321 | 0.804931 | 1.70E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene53321 | 0.840778 | 7.08E-58 | -1.69E-46 |
| MS.gene052488 | MS.gene53321 | 0.80013 | 1.66E-48 | -1.69E-46 |
| MS.gene052516 | MS.gene53321 | 0.861367 | 1.08E-63 | -1.69E-46 |
| MS.gene052519 | MS.gene53321 | 0.856993 | 2.21E-62 | -1.69E-46 |
| MS.gene052534 | MS.gene53321 | 0.834815 | 2.41E-56 | -1.69E-46 |
| MS.gene052582 | MS.gene53321 | 0.829726 | 4.38E-55 | -1.69E-46 |
| MS.gene052584 | MS.gene53321 | 0.83907 | 1.97E-57 | -1.69E-46 |
| MS.gene052586 | MS.gene53321 | 0.804274 | 2.34E-49 | -1.69E-46 |
| MS.gene052611 | MS.gene53321 | 0.832462 | 9.32E-56 | -1.69E-46 |
| MS.gene052773 | MS.gene53321 | 0.855015 | 8.37E-62 | -1.69E-46 |
| MS.gene052863 | MS.gene53321 | 0.856189 | 3.81E-62 | -1.69E-46 |
| MS.gene052867 | MS.gene53321 | 0.808124 | 3.61E-50 | -1.69E-46 |
| MS.gene052896 | MS.gene53321 | 0.852532 | 4.34E-61 | -1.69E-46 |
| MS.gene053008 | MS.gene53321 | 0.832144 | 1.12E-55 | -1.69E-46 |
| MS.gene053079 | MS.gene53321 | 0.812704 | 3.71E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene53321 | 0.813401 | 2.61E-51 | -1.69E-46 |
| MS.gene053164 | MS.gene53321 | 0.813239 | 2.83E-51 | -1.69E-46 |
| MS.gene053166 | MS.gene53321 | 0.833329 | 5.68E-56 | -1.69E-46 |
| MS.gene053221 | MS.gene53321 | 0.821907 | 3.15E-53 | -1.69E-46 |
| MS.gene053226 | MS.gene53321 | 0.868392 | 6.76E-66 | -1.69E-46 |
| MS.gene053296 | MS.gene53321 | 0.81081 | 9.58E-51 | -1.69E-46 |
| MS.gene053331 | MS.gene53321 | 0.805472 | 1.31E-49 | -1.69E-46 |
| MS.gene05350 | MS.gene53321 | 0.81623 | 6.16E-52 | -1.69E-46 |
| MS.gene053621 | MS.gene53321 | 0.80558 | 1.25E-49 | -1.69E-46 |
| MS.gene053641 | MS.gene53321 | 0.801847 | 7.42E-49 | -1.69E-46 |
| MS.gene053966 | MS.gene53321 | 0.810429 | 1.16E-50 | -1.69E-46 |
| MS.gene054139 | MS.gene53321 | 0.806638 | 7.47E-50 | -1.69E-46 |
| MS.gene05414 | MS.gene53321 | 0.814609 | 1.41E-51 | -1.69E-46 |
| MS.gene05415 | MS.gene53321 | 0.821585 | 3.73E-53 | -1.69E-46 |
| MS.gene05422 | MS.gene53321 | 0.809455 | 1.88E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene53321 | 0.839226 | 1.80E-57 | -1.69E-46 |
| MS.gene05423 | MS.gene53321 | 0.837233 | 5.87E-57 | -1.69E-46 |
| MS.gene054291 | MS.gene53321 | -0.829487 | 5.01E-55 | -1.69E-46 |
| MS.gene054467 | MS.gene53321 | 0.839192 | 1.84E-57 | -1.69E-46 |
| MS.gene054468 | MS.gene53321 | 0.83897 | 2.10E-57 | -1.69E-46 |
| MS.gene054472 | MS.gene53321 | 0.821033 | 5.01E-53 | -1.69E-46 |
| MS.gene054576 | MS.gene53321 | 0.815264 | 1.01E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene53321 | 0.812804 | 3.53E-51 | -1.69E-46 |
| MS.gene05458 | MS.gene53321 | 0.842948 | 1.89E-58 | -1.69E-46 |
| MS.gene054651 | MS.gene53321 | 0.853895 | 1.77E-61 | -1.69E-46 |
| MS.gene05468 | MS.gene53321 | 0.844566 | 6.99E-59 | -1.69E-46 |
| MS.gene054775 | MS.gene53321 | 0.864047 | 1.61E-64 | -1.69E-46 |
| MS.gene054801 | MS.gene53321 | 0.820404 | 6.98E-53 | -1.69E-46 |
| MS.gene054991 | MS.gene53321 | 0.836665 | 8.19E-57 | -1.69E-46 |
| MS.gene055083 | MS.gene53321 | 0.800946 | 1.13E-48 | -1.69E-46 |
| MS.gene055144 | MS.gene53321 | 0.820672 | 6.06E-53 | -1.69E-46 |
| MS.gene055166 | MS.gene53321 | 0.809616 | 1.73E-50 | -1.69E-46 |
| MS.gene055227 | MS.gene53321 | 0.836084 | 1.15E-56 | -1.69E-46 |
| MS.gene055228 | MS.gene53321 | 0.82567 | 4.13E-54 | -1.69E-46 |
| MS.gene055268 | MS.gene53321 | 0.80465 | 1.95E-49 | -1.69E-46 |
| MS.gene055310 | MS.gene53321 | 0.804134 | 2.50E-49 | -1.69E-46 |
| MS.gene055313 | MS.gene53321 | 0.815492 | 8.99E-52 | -1.69E-46 |
| MS.gene055356 | MS.gene53321 | 0.812099 | 5.03E-51 | -1.69E-46 |
| MS.gene055357 | MS.gene53321 | 0.813234 | 2.84E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene53321 | 0.852431 | 4.64E-61 | -1.69E-46 |
| MS.gene055466 | MS.gene53321 | 0.856236 | 3.69E-62 | -1.69E-46 |
| MS.gene055468 | MS.gene53321 | 0.83832 | 3.08E-57 | -1.69E-46 |
| MS.gene055469 | MS.gene53321 | 0.807183 | 5.73E-50 | -1.69E-46 |
| MS.gene055512 | MS.gene53321 | 0.82075 | 5.81E-53 | -1.69E-46 |
| MS.gene055513 | MS.gene53321 | 0.831685 | 1.45E-55 | -1.69E-46 |
| MS.gene055537 | MS.gene53321 | 0.800195 | 1.61E-48 | -1.69E-46 |
| MS.gene055580 | MS.gene53321 | -0.807112 | 5.93E-50 | -1.69E-46 |
| MS.gene055586 | MS.gene53321 | 0.82011 | 8.15E-53 | -1.69E-46 |
| MS.gene055589 | MS.gene53321 | 0.810569 | 1.08E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene53321 | 0.805029 | 1.63E-49 | -1.69E-46 |
| MS.gene055778 | MS.gene53321 | 0.82072 | 5.91E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene53321 | 0.859722 | 3.40E-63 | -1.69E-46 |
| MS.gene055926 | MS.gene53321 | 0.857168 | 1.96E-62 | -1.69E-46 |
| MS.gene056005 | MS.gene53321 | 0.80896 | 2.40E-50 | -1.69E-46 |
| MS.gene05604 | MS.gene53321 | 0.868143 | 8.13E-66 | -1.69E-46 |
| MS.gene056050 | MS.gene53321 | 0.841255 | 5.31E-58 | -1.69E-46 |
| MS.gene056162 | MS.gene53321 | 0.804624 | 1.98E-49 | -1.69E-46 |
| MS.gene056173 | MS.gene53321 | 0.837511 | 4.98E-57 | -1.69E-46 |
| MS.gene056174 | MS.gene53321 | 0.819373 | 1.20E-52 | -1.69E-46 |
| MS.gene056308 | MS.gene53321 | 0.841696 | 4.06E-58 | -1.69E-46 |
| MS.gene056366 | MS.gene53321 | 0.835233 | 1.89E-56 | -1.69E-46 |
| MS.gene056373 | MS.gene53321 | 0.816155 | 6.40E-52 | -1.69E-46 |
| MS.gene056506 | MS.gene53321 | 0.819867 | 9.27E-53 | -1.69E-46 |
| MS.gene056507 | MS.gene53321 | 0.80267 | 5.02E-49 | -1.69E-46 |
| MS.gene056553 | MS.gene53321 | 0.827522 | 1.49E-54 | -1.69E-46 |
| MS.gene056595 | MS.gene53321 | 0.840779 | 7.08E-58 | -1.69E-46 |
| MS.gene05686 | MS.gene53321 | 0.836847 | 7.36E-57 | -1.69E-46 |
| MS.gene05690 | MS.gene53321 | 0.805412 | 1.35E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene53321 | 0.848222 | 7.03E-60 | -1.69E-46 |
| MS.gene057034 | MS.gene53321 | 0.800615 | 1.33E-48 | -1.69E-46 |
| MS.gene057060 | MS.gene53321 | 0.805385 | 1.37E-49 | -1.69E-46 |
| MS.gene057132 | MS.gene53321 | 0.827613 | 1.42E-54 | -1.69E-46 |
| MS.gene057208 | MS.gene53321 | 0.823908 | 1.08E-53 | -1.69E-46 |
| MS.gene057360 | MS.gene53321 | 0.808479 | 3.04E-50 | -1.69E-46 |
| MS.gene057444 | MS.gene53321 | 0.819159 | 1.34E-52 | -1.69E-46 |
| MS.gene057446 | MS.gene53321 | 0.80033 | 1.52E-48 | -1.69E-46 |
| MS.gene057598 | MS.gene53321 | 0.816812 | 4.56E-52 | -1.69E-46 |
| MS.gene057693 | MS.gene53321 | 0.825219 | 5.28E-54 | -1.69E-46 |
| MS.gene057855 | MS.gene53321 | 0.800854 | 1.19E-48 | -1.69E-46 |
| MS.gene057915 | MS.gene53321 | 0.858145 | 1.01E-62 | -1.69E-46 |
| MS.gene057921 | MS.gene53321 | 0.804307 | 2.30E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene53321 | 0.830593 | 2.69E-55 | -1.69E-46 |
| MS.gene058037 | MS.gene53321 | 0.807869 | 4.09E-50 | -1.69E-46 |
| MS.gene058339 | MS.gene53321 | 0.824402 | 8.23E-54 | -1.69E-46 |
| MS.gene05847 | MS.gene53321 | 0.811146 | 8.10E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene53321 | 0.873888 | 1.04E-67 | -1.69E-46 |
| MS.gene058636 | MS.gene53321 | 0.816404 | 5.63E-52 | -1.69E-46 |
| MS.gene058647 | MS.gene53321 | 0.801091 | 1.06E-48 | -1.69E-46 |
| MS.gene058829 | MS.gene53321 | 0.833654 | 4.71E-56 | -1.69E-46 |
| MS.gene058979 | MS.gene53321 | 0.837077 | 6.43E-57 | -1.69E-46 |
| MS.gene05897 | MS.gene53321 | 0.824623 | 7.30E-54 | -1.69E-46 |
| MS.gene059272 | MS.gene53321 | 0.825651 | 4.17E-54 | -1.69E-46 |
| MS.gene059283 | MS.gene53321 | 0.804383 | 2.22E-49 | -1.69E-46 |
| MS.gene059438 | MS.gene53321 | 0.801887 | 7.28E-49 | -1.69E-46 |
| MS.gene059439 | MS.gene53321 | 0.850573 | 1.56E-60 | -1.69E-46 |
| MS.gene059570 | MS.gene53321 | 0.838157 | 3.40E-57 | -1.69E-46 |
| MS.gene059584 | MS.gene53321 | 0.800638 | 1.31E-48 | -1.69E-46 |
| MS.gene059601 | MS.gene53321 | 0.801064 | 1.07E-48 | -1.69E-46 |
| MS.gene059602 | MS.gene53321 | 0.805899 | 1.07E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene53321 | 0.845301 | 4.42E-59 | -1.69E-46 |
| MS.gene059725 | MS.gene53321 | 0.834621 | 2.70E-56 | -1.69E-46 |
| MS.gene059842 | MS.gene53321 | 0.83972 | 1.34E-57 | -1.69E-46 |
| MS.gene059937 | MS.gene53321 | 0.803005 | 4.28E-49 | -1.69E-46 |
| MS.gene060011 | MS.gene53321 | 0.810985 | 8.78E-51 | -1.69E-46 |
| MS.gene060167 | MS.gene53321 | 0.817294 | 3.55E-52 | -1.69E-46 |
| MS.gene060312 | MS.gene53321 | 0.814373 | 1.59E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene53321 | 0.850845 | 1.30E-60 | -1.69E-46 |
| MS.gene06052 | MS.gene53321 | 0.850772 | 1.37E-60 | -1.69E-46 |
| MS.gene060587 | MS.gene53321 | 0.808692 | 2.73E-50 | -1.69E-46 |
| MS.gene060637 | MS.gene53321 | 0.80151 | 8.70E-49 | -1.69E-46 |
| MS.gene060781 | MS.gene53321 | 0.81755 | 3.11E-52 | -1.69E-46 |
| MS.gene060802 | MS.gene53321 | 0.804097 | 2.54E-49 | -1.69E-46 |
| MS.gene060803 | MS.gene53321 | 0.831764 | 1.39E-55 | -1.69E-46 |
| MS.gene060935 | MS.gene53321 | 0.803803 | 2.93E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53321.t1 | MTR_8g019660 | 93.484 | 353 | 12 | 3 | 1 | 347 | 58 | 405 | 0.0 | 562 |
| MS.gene53321.t1 | MTR_3g104880 | 42.960 | 277 | 109 | 10 | 73 | 341 | 136 | 371 | 1.64e-39 | 144 |
| MS.gene53321.t1 | MTR_4g125620 | 37.644 | 348 | 139 | 14 | 10 | 347 | 65 | 344 | 4.60e-28 | 112 |
| MS.gene53321.t1 | MTR_1g015275 | 39.373 | 287 | 110 | 8 | 67 | 333 | 123 | 365 | 4.30e-22 | 96.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53321.t1 | AT3G13980 | 51.636 | 275 | 88 | 10 | 78 | 347 | 123 | 357 | 2.51e-64 | 208 |
| MS.gene53321.t1 | AT1G54200 | 48.175 | 274 | 112 | 9 | 76 | 342 | 115 | 365 | 1.69e-54 | 183 |
| MS.gene53321.t1 | AT3G42800 | 37.719 | 228 | 111 | 13 | 125 | 347 | 140 | 341 | 2.39e-25 | 104 |
| MS.gene53321.t1 | AT5G12050 | 39.726 | 219 | 98 | 9 | 134 | 333 | 153 | 356 | 9.48e-12 | 65.9 |
Find 40 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGATAATTTATCAGTAAT+TGG | 0.114503 | 8.4:-73575979 | MS.gene53321:CDS |
| TTCAATCCCAATAGAGTTAT+TGG | 0.119420 | 8.4:-73575910 | MS.gene53321:CDS |
| ATGTGATGCAAGAGAGTTTA+AGG | 0.174902 | 8.4:-73576134 | MS.gene53321:CDS |
| TCAGAAACACTTGTTCTAAT+TGG | 0.223959 | 8.4:+73576669 | None:intergenic |
| ACCATTTCCAATAACTCTAT+TGG | 0.241032 | 8.4:+73575903 | None:intergenic |
| AACATTGATTCATTTCAATA+TGG | 0.291124 | 8.4:-73576639 | MS.gene53321:CDS |
| AACTACTACAAATTCTGTTT+TGG | 0.296788 | 8.4:-73576388 | MS.gene53321:CDS |
| TCTGATTCAGAATCATTCTA+TGG | 0.300272 | 8.4:-73576708 | MS.gene53321:CDS |
| ACACTTGTTCTAATTGGTTT+TGG | 0.300946 | 8.4:+73576675 | None:intergenic |
| CCCAATAGAGTTATTGGAAA+TGG | 0.315105 | 8.4:-73575904 | MS.gene53321:CDS |
| CCATTTCCAATAACTCTATT+GGG | 0.327955 | 8.4:+73575904 | None:intergenic |
| AAGCAAGAAAGGTGGAGAAT+TGG | 0.331562 | 8.4:-73576866 | MS.gene53321:CDS |
| GAACAGAATGGTTATGGTTA+AGG | 0.337246 | 8.4:+73576604 | None:intergenic |
| TGGATAATTTATCAGTAATT+GGG | 0.347307 | 8.4:-73575978 | MS.gene53321:CDS |
| GAGTTTGAACAGAATGGTTA+TGG | 0.352789 | 8.4:+73576598 | None:intergenic |
| TATTGAAATGAATCAATGTT+TGG | 0.355365 | 8.4:+73576642 | None:intergenic |
| TCATCAACAATTACACTAAC+AGG | 0.363408 | 8.4:+73576252 | None:intergenic |
| ATACAAAATTCTCAAAGCTT+TGG | 0.378204 | 8.4:+73576536 | None:intergenic |
| AGAGTAGTAGTGAAGAATTA+AGG | 0.394514 | 8.4:-73576161 | MS.gene53321:CDS |
| GAATTTGCATGAAGGTGAAA+AGG | 0.410666 | 8.4:-73576205 | MS.gene53321:CDS |
| TAGTTCTGATTTGTTTGAAT+TGG | 0.413467 | 8.4:-73575998 | MS.gene53321:CDS |
| TATCAGTAATTGGGATTGAA+AGG | 0.444002 | 8.4:-73575969 | MS.gene53321:CDS |
| TTAGCACCTGATCTTGATGA+AGG | 0.453605 | 8.4:+73576294 | None:intergenic |
| AAAACTCCTTCATCAAGATC+AGG | 0.481984 | 8.4:-73576300 | MS.gene53321:CDS |
| TCTTCAGAATCAAGTTCAGC+TGG | 0.492878 | 8.4:-73576744 | MS.gene53321:CDS |
| AATTTGCATGAAGGTGAAAA+GGG | 0.494062 | 8.4:-73576204 | MS.gene53321:CDS |
| AAGGGTTCTTCAATCTATGA+TGG | 0.496294 | 8.4:-73576186 | MS.gene53321:CDS |
| AAACTTCAGAAAAGCAAGAA+AGG | 0.514735 | 8.4:-73576877 | MS.gene53321:CDS |
| TGCAAATTCTTGTGTCCACA+AGG | 0.543709 | 8.4:+73576219 | None:intergenic |
| TGTGATGCAAGAGAGTTTAA+GGG | 0.547284 | 8.4:-73576133 | MS.gene53321:CDS |
| GATGAAGATGAAGATGATGA+TGG | 0.547702 | 8.4:-73576033 | MS.gene53321:CDS |
| TGGAATGAGAAAGTTGTGAA+TGG | 0.552681 | 8.4:-73576846 | MS.gene53321:CDS |
| AGAAGATGAACTTGAATGCA+TGG | 0.580776 | 8.4:+73576764 | None:intergenic |
| TCAGAATCAAGTTCAGCTGG+TGG | 0.600576 | 8.4:-73576741 | MS.gene53321:CDS |
| AAATGAGTAGTTTCATACAC+AGG | 0.610521 | 8.4:+73575931 | None:intergenic |
| CTTCAGAAAAGCAAGAAAGG+TGG | 0.635352 | 8.4:-73576874 | MS.gene53321:CDS |
| ACAAAGCAACCTATTTCACC+TGG | 0.653006 | 8.4:-73576498 | MS.gene53321:CDS |
| GATGAAGATTGTAGACCTTG+TGG | 0.657954 | 8.4:-73576234 | MS.gene53321:CDS |
| GGACACAAGAATTTGCATGA+AGG | 0.670978 | 8.4:-73576213 | MS.gene53321:CDS |
| CAGGTGCTAAAAGATCAGTG+AGG | 0.837686 | 8.4:-73576281 | MS.gene53321:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTGAAATGAATCAATGTT+TGG | + | chr8.4:73576164-73576183 | None:intergenic | 20.0% |
| !! | TGGATAATTTATCAGTAATT+GGG | - | chr8.4:73576825-73576844 | MS.gene53321:CDS | 20.0% |
| !! | TTGGATAATTTATCAGTAAT+TGG | - | chr8.4:73576824-73576843 | MS.gene53321:CDS | 20.0% |
| !!! | AACATTGATTCATTTCAATA+TGG | - | chr8.4:73576164-73576183 | MS.gene53321:CDS | 20.0% |
| !!! | TGATGGTATTTTTGATTTTT+TGG | + | chr8.4:73576381-73576400 | None:intergenic | 20.0% |
| ! | ATACAAAATTCTCAAAGCTT+TGG | + | chr8.4:73576270-73576289 | None:intergenic | 25.0% |
| ! | CAAAAACCAAAAAATGAGAA+TGG | - | chr8.4:73576227-73576246 | MS.gene53321:CDS | 25.0% |
| !! | AACTACTACAAATTCTGTTT+TGG | - | chr8.4:73576415-73576434 | MS.gene53321:CDS | 25.0% |
| !!! | AAAGCTTTGAGAATTTTGTA+TGG | - | chr8.4:73576269-73576288 | MS.gene53321:CDS | 25.0% |
| !!! | AATTCACTTTTCAATTCTAG+TGG | - | chr8.4:73576350-73576369 | MS.gene53321:CDS | 25.0% |
| !!! | TAGTTCTGATTTGTTTGAAT+TGG | - | chr8.4:73576805-73576824 | MS.gene53321:CDS | 25.0% |
| AAACTTCAGAAAAGCAAGAA+AGG | - | chr8.4:73575926-73575945 | MS.gene53321:CDS | 30.0% | |
| AAATGAGTAGTTTCATACAC+AGG | + | chr8.4:73576875-73576894 | None:intergenic | 30.0% | |
| AATTTGCATGAAGGTGAAAA+GGG | - | chr8.4:73576599-73576618 | MS.gene53321:CDS | 30.0% | |
| ACCATTTCCAATAACTCTAT+TGG | + | chr8.4:73576903-73576922 | None:intergenic | 30.0% | |
| AGAGTAGTAGTGAAGAATTA+AGG | - | chr8.4:73576642-73576661 | MS.gene53321:CDS | 30.0% | |
| CCAAAAAATGAGAATGGTTT+AGG | - | chr8.4:73576233-73576252 | MS.gene53321:CDS | 30.0% | |
| CCATTTCCAATAACTCTATT+GGG | + | chr8.4:73576902-73576921 | None:intergenic | 30.0% | |
| TATCAGTAATTGGGATTGAA+AGG | - | chr8.4:73576834-73576853 | MS.gene53321:CDS | 30.0% | |
| TCAGAAACACTTGTTCTAAT+TGG | + | chr8.4:73576137-73576156 | None:intergenic | 30.0% | |
| TCATCAACAATTACACTAAC+AGG | + | chr8.4:73576554-73576573 | None:intergenic | 30.0% | |
| TCTGATTCAGAATCATTCTA+TGG | - | chr8.4:73576095-73576114 | MS.gene53321:CDS | 30.0% | |
| TTCAATCCCAATAGAGTTAT+TGG | - | chr8.4:73576893-73576912 | MS.gene53321:CDS | 30.0% | |
| !! | ACACTTGTTCTAATTGGTTT+TGG | + | chr8.4:73576131-73576150 | None:intergenic | 30.0% |
| !!! | CCTAAACCATTCTCATTTTT+TGG | + | chr8.4:73576236-73576255 | None:intergenic | 30.0% |
| !!! | GTTTTTGAGTTTGAACAGAA+TGG | + | chr8.4:73576214-73576233 | None:intergenic | 30.0% |
| !!! | TTTCTTGCTTTTCTGAAGTT+TGG | + | chr8.4:73575927-73575946 | None:intergenic | 30.0% |
| AAAACTCCTTCATCAAGATC+AGG | - | chr8.4:73576503-73576522 | MS.gene53321:CDS | 35.0% | |
| AAGGGTTCTTCAATCTATGA+TGG | - | chr8.4:73576617-73576636 | MS.gene53321:CDS | 35.0% | |
| AGAAGATGAACTTGAATGCA+TGG | + | chr8.4:73576042-73576061 | None:intergenic | 35.0% | |
| ATGTGATGCAAGAGAGTTTA+AGG | - | chr8.4:73576669-73576688 | MS.gene53321:CDS | 35.0% | |
| CCCAATAGAGTTATTGGAAA+TGG | - | chr8.4:73576899-73576918 | MS.gene53321:CDS | 35.0% | |
| GAACAGAATGGTTATGGTTA+AGG | + | chr8.4:73576202-73576221 | None:intergenic | 35.0% | |
| GAATTTGCATGAAGGTGAAA+AGG | - | chr8.4:73576598-73576617 | MS.gene53321:CDS | 35.0% | |
| GAGTTTGAACAGAATGGTTA+TGG | + | chr8.4:73576208-73576227 | None:intergenic | 35.0% | |
| GATGAAGATGAAGATGATGA+TGG | - | chr8.4:73576770-73576789 | MS.gene53321:CDS | 35.0% | |
| TGGAATGAGAAAGTTGTGAA+TGG | - | chr8.4:73575957-73575976 | MS.gene53321:CDS | 35.0% | |
| TGTGATGCAAGAGAGTTTAA+GGG | - | chr8.4:73576670-73576689 | MS.gene53321:CDS | 35.0% | |
| ! | ATTTTTGCACCAGGTGAAAT+AGG | + | chr8.4:73576317-73576336 | None:intergenic | 35.0% |
| !! | CATCAGTGTCTTCTTTTTCA+AGG | - | chr8.4:73576468-73576487 | MS.gene53321:CDS | 35.0% |
| !! | GTTTTTGTAGCTGTTGATGA+TGG | + | chr8.4:73576398-73576417 | None:intergenic | 35.0% |
| AAGCAAGAAAGGTGGAGAAT+TGG | - | chr8.4:73575937-73575956 | MS.gene53321:CDS | 40.0% | |
| ACAAAGCAACCTATTTCACC+TGG | - | chr8.4:73576305-73576324 | MS.gene53321:CDS | 40.0% | |
| CTTCAGAAAAGCAAGAAAGG+TGG | - | chr8.4:73575929-73575948 | MS.gene53321:CDS | 40.0% | |
| GATGAAGATTGTAGACCTTG+TGG | - | chr8.4:73576569-73576588 | MS.gene53321:CDS | 40.0% | |
| GGACACAAGAATTTGCATGA+AGG | - | chr8.4:73576590-73576609 | MS.gene53321:CDS | 40.0% | |
| TCTTCAGAATCAAGTTCAGC+TGG | - | chr8.4:73576059-73576078 | MS.gene53321:CDS | 40.0% | |
| TGCAAATTCTTGTGTCCACA+AGG | + | chr8.4:73576587-73576606 | None:intergenic | 40.0% | |
| TTAGCACCTGATCTTGATGA+AGG | + | chr8.4:73576512-73576531 | None:intergenic | 40.0% | |
| ! | AAGCTAGCAATTTTTGCACC+AGG | + | chr8.4:73576326-73576345 | None:intergenic | 40.0% |
| CAGGTGCTAAAAGATCAGTG+AGG | - | chr8.4:73576522-73576541 | MS.gene53321:CDS | 45.0% | |
| TCAGAATCAAGTTCAGCTGG+TGG | - | chr8.4:73576062-73576081 | MS.gene53321:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 73575891 | 73576934 | 73575891 | ID=MS.gene53321 |
| chr8.4 | mRNA | 73575891 | 73576934 | 73575891 | ID=MS.gene53321.t1;Parent=MS.gene53321 |
| chr8.4 | exon | 73575891 | 73576934 | 73575891 | ID=MS.gene53321.t1.exon1;Parent=MS.gene53321.t1 |
| chr8.4 | CDS | 73575891 | 73576934 | 73575891 | ID=cds.MS.gene53321.t1;Parent=MS.gene53321.t1 |
| Gene Sequence |
| Protein sequence |
