AlfalfaGEDB | MS.gene72400

M. sativa cultivar XinJiangDaYe / MS.gene72400

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF13912.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72400.t1	RHN63233.1	83.6	116	15	2	29	144	204	315	3.60E-40	174.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72400.t1	Q9M202	38.2	123	59	3	30	141	170	286	7.7e-15	81.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72400.t1	G7JIV8	83.6	116	15	2	29	144	204	315	2.6e-40	174.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene72400.t1	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene72400	0.81961	1.06E-52	-1.69E-46
MS.gene049159	MS.gene72400	0.846625	1.93E-59	-1.69E-46
MS.gene049190	MS.gene72400	0.801138	1.04E-48	-1.69E-46
MS.gene049254	MS.gene72400	0.867895	9.77E-66	-1.69E-46
MS.gene049343	MS.gene72400	0.809919	1.49E-50	-1.69E-46
MS.gene049404	MS.gene72400	0.865654	5.05E-65	-1.69E-46
MS.gene049435	MS.gene72400	0.827032	1.96E-54	-1.69E-46
MS.gene049452	MS.gene72400	0.831377	1.73E-55	-1.69E-46
MS.gene049501	MS.gene72400	0.837065	6.48E-57	-1.69E-46
MS.gene049711	MS.gene72400	0.821116	4.79E-53	-1.69E-46
MS.gene049850	MS.gene72400	0.82639	2.79E-54	-1.69E-46
MS.gene049983	MS.gene72400	0.813537	2.43E-51	-1.69E-46
MS.gene050026	MS.gene72400	0.842662	2.25E-58	-1.69E-46
MS.gene050073	MS.gene72400	0.809326	2.00E-50	-1.69E-46
MS.gene05016	MS.gene72400	0.80126	9.79E-49	-1.69E-46
MS.gene050251	MS.gene72400	0.80777	4.30E-50	-1.69E-46
MS.gene050273	MS.gene72400	0.826429	2.73E-54	-1.69E-46
MS.gene050442	MS.gene72400	0.820143	8.01E-53	-1.69E-46
MS.gene050559	MS.gene72400	0.843896	1.06E-58	-1.69E-46
MS.gene050561	MS.gene72400	0.847476	1.13E-59	-1.69E-46
MS.gene050562	MS.gene72400	0.841703	4.05E-58	-1.69E-46
MS.gene050657	MS.gene72400	0.822305	2.54E-53	-1.69E-46
MS.gene050719	MS.gene72400	0.805405	1.36E-49	-1.69E-46
MS.gene050750	MS.gene72400	0.801405	9.14E-49	-1.69E-46
MS.gene050759	MS.gene72400	0.866189	3.42E-65	-1.69E-46
MS.gene050780	MS.gene72400	0.803803	2.93E-49	-1.69E-46
MS.gene050826	MS.gene72400	0.800036	1.74E-48	-1.69E-46
MS.gene050962	MS.gene72400	0.857538	1.52E-62	-1.69E-46
MS.gene051181	MS.gene72400	0.816369	5.73E-52	-1.69E-46
MS.gene051198	MS.gene72400	0.854256	1.39E-61	-1.69E-46
MS.gene051247	MS.gene72400	0.839752	1.31E-57	-1.69E-46
MS.gene051279	MS.gene72400	0.879471	1.21E-69	-1.69E-46
MS.gene051388	MS.gene72400	0.8077	4.45E-50	-1.69E-46
MS.gene051490	MS.gene72400	0.812805	3.52E-51	-1.69E-46
MS.gene051543	MS.gene72400	0.805785	1.13E-49	-1.69E-46
MS.gene051594	MS.gene72400	0.80887	2.50E-50	-1.69E-46
MS.gene051719	MS.gene72400	0.820272	7.49E-53	-1.69E-46
MS.gene051720	MS.gene72400	0.825237	5.23E-54	-1.69E-46
MS.gene051744	MS.gene72400	0.885046	1.14E-71	-1.69E-46
MS.gene051800	MS.gene72400	0.827088	1.90E-54	-1.69E-46
MS.gene051859	MS.gene72400	0.818535	1.86E-52	-1.69E-46
MS.gene052037	MS.gene72400	0.824653	7.18E-54	-1.69E-46
MS.gene05215	MS.gene72400	0.829445	5.13E-55	-1.69E-46
MS.gene052239	MS.gene72400	-0.80032	1.52E-48	-1.69E-46
MS.gene052333	MS.gene72400	0.824349	8.47E-54	-1.69E-46
MS.gene052459	MS.gene72400	0.803672	3.12E-49	-1.69E-46
MS.gene052463	MS.gene72400	0.809419	1.91E-50	-1.69E-46
MS.gene052488	MS.gene72400	0.831408	1.70E-55	-1.69E-46
MS.gene052516	MS.gene72400	0.829697	4.45E-55	-1.69E-46
MS.gene052519	MS.gene72400	0.843757	1.15E-58	-1.69E-46
MS.gene052561	MS.gene72400	0.805605	1.23E-49	-1.69E-46
MS.gene052582	MS.gene72400	0.857857	1.23E-62	-1.69E-46
MS.gene052584	MS.gene72400	0.873967	9.75E-68	-1.69E-46
MS.gene052586	MS.gene72400	0.802739	4.86E-49	-1.69E-46
MS.gene052611	MS.gene72400	0.821032	5.01E-53	-1.69E-46
MS.gene052665	MS.gene72400	0.806909	6.54E-50	-1.69E-46
MS.gene052773	MS.gene72400	0.833852	4.20E-56	-1.69E-46
MS.gene052863	MS.gene72400	0.821096	4.84E-53	-1.69E-46
MS.gene053079	MS.gene72400	0.858727	6.76E-63	-1.69E-46
MS.gene053144	MS.gene72400	0.823771	1.16E-53	-1.69E-46
MS.gene053166	MS.gene72400	0.807173	5.75E-50	-1.69E-46
MS.gene053226	MS.gene72400	0.878276	3.20E-69	-1.69E-46
MS.gene053296	MS.gene72400	0.839221	1.80E-57	-1.69E-46
MS.gene053621	MS.gene72400	0.840905	6.56E-58	-1.69E-46
MS.gene05364	MS.gene72400	0.808259	3.38E-50	-1.69E-46
MS.gene053883	MS.gene72400	0.818493	1.91E-52	-1.69E-46
MS.gene053998	MS.gene72400	0.828729	7.65E-55	-1.69E-46
MS.gene05414	MS.gene72400	0.816719	4.78E-52	-1.69E-46
MS.gene05415	MS.gene72400	0.827149	1.83E-54	-1.69E-46
MS.gene054236	MS.gene72400	0.834069	3.71E-56	-1.69E-46
MS.gene05423	MS.gene72400	0.830286	3.20E-55	-1.69E-46
MS.gene054291	MS.gene72400	-0.824933	6.17E-54	-1.69E-46
MS.gene054467	MS.gene72400	0.833185	6.17E-56	-1.69E-46
MS.gene054468	MS.gene72400	0.814151	1.78E-51	-1.69E-46
MS.gene054576	MS.gene72400	0.856162	3.87E-62	-1.69E-46
MS.gene054584	MS.gene72400	0.826806	2.22E-54	-1.69E-46
MS.gene05458	MS.gene72400	0.804613	1.99E-49	-1.69E-46
MS.gene054651	MS.gene72400	0.892396	1.65E-74	-1.69E-46
MS.gene054775	MS.gene72400	0.841989	3.40E-58	-1.69E-46
MS.gene054801	MS.gene72400	0.807771	4.30E-50	-1.69E-46
MS.gene054991	MS.gene72400	0.820771	5.75E-53	-1.69E-46
MS.gene055166	MS.gene72400	0.818598	1.80E-52	-1.69E-46
MS.gene055222	MS.gene72400	0.809243	2.08E-50	-1.69E-46
MS.gene055227	MS.gene72400	0.84312	1.70E-58	-1.69E-46
MS.gene055228	MS.gene72400	0.832151	1.11E-55	-1.69E-46
MS.gene055357	MS.gene72400	0.814087	1.84E-51	-1.69E-46
MS.gene055464	MS.gene72400	0.80048	1.41E-48	-1.69E-46
MS.gene055466	MS.gene72400	0.816175	6.33E-52	-1.69E-46
MS.gene055512	MS.gene72400	0.823543	1.31E-53	-1.69E-46
MS.gene055580	MS.gene72400	-0.80181	7.55E-49	-1.69E-46
MS.gene055586	MS.gene72400	0.846194	2.53E-59	-1.69E-46
MS.gene055589	MS.gene72400	0.836453	9.28E-57	-1.69E-46
MS.gene055778	MS.gene72400	0.813131	2.99E-51	-1.69E-46
MS.gene055818	MS.gene72400	0.816792	4.61E-52	-1.69E-46
MS.gene055926	MS.gene72400	0.835401	1.71E-56	-1.69E-46
MS.gene05604	MS.gene72400	0.81609	6.62E-52	-1.69E-46
MS.gene056050	MS.gene72400	0.853619	2.12E-61	-1.69E-46
MS.gene056162	MS.gene72400	0.816409	5.61E-52	-1.69E-46
MS.gene056173	MS.gene72400	0.829947	3.87E-55	-1.69E-46
MS.gene056174	MS.gene72400	0.853201	2.79E-61	-1.69E-46
MS.gene056252	MS.gene72400	0.80285	4.61E-49	-1.69E-46
MS.gene056366	MS.gene72400	0.839947	1.17E-57	-1.69E-46
MS.gene056506	MS.gene72400	0.841188	5.53E-58	-1.69E-46
MS.gene056507	MS.gene72400	0.814156	1.78E-51	-1.69E-46
MS.gene056595	MS.gene72400	0.823011	1.74E-53	-1.69E-46
MS.gene05659	MS.gene72400	0.801413	9.11E-49	-1.69E-46
MS.gene056776	MS.gene72400	0.806877	6.65E-50	-1.69E-46
MS.gene05686	MS.gene72400	0.85057	1.56E-60	-1.69E-46
MS.gene05690	MS.gene72400	0.809192	2.14E-50	-1.69E-46
MS.gene057034	MS.gene72400	0.815873	7.39E-52	-1.69E-46
MS.gene057060	MS.gene72400	0.810806	9.60E-51	-1.69E-46
MS.gene057208	MS.gene72400	0.825527	4.47E-54	-1.69E-46
MS.gene057314	MS.gene72400	0.824904	6.27E-54	-1.69E-46
MS.gene057414	MS.gene72400	0.821931	3.11E-53	-1.69E-46
MS.gene057444	MS.gene72400	0.87931	1.38E-69	-1.69E-46
MS.gene057693	MS.gene72400	0.807048	6.11E-50	-1.69E-46
MS.gene057855	MS.gene72400	0.804709	1.90E-49	-1.69E-46
MS.gene057869	MS.gene72400	0.83208	1.16E-55	-1.69E-46
MS.gene057915	MS.gene72400	0.826874	2.13E-54	-1.69E-46
MS.gene058008	MS.gene72400	0.81683	4.52E-52	-1.69E-46
MS.gene058009	MS.gene72400	0.836538	8.82E-57	-1.69E-46
MS.gene058010	MS.gene72400	0.863061	3.26E-64	-1.69E-46
MS.gene058037	MS.gene72400	0.820907	5.35E-53	-1.69E-46
MS.gene058294	MS.gene72400	0.819489	1.13E-52	-1.69E-46
MS.gene058548	MS.gene72400	0.812873	3.41E-51	-1.69E-46
MS.gene058625	MS.gene72400	0.84591	3.02E-59	-1.69E-46
MS.gene058636	MS.gene72400	0.809931	1.48E-50	-1.69E-46
MS.gene058783	MS.gene72400	0.804315	2.29E-49	-1.69E-46
MS.gene058829	MS.gene72400	0.80784	4.15E-50	-1.69E-46
MS.gene058862	MS.gene72400	0.801127	1.04E-48	-1.69E-46
MS.gene058979	MS.gene72400	0.859402	4.24E-63	-1.69E-46
MS.gene059015	MS.gene72400	0.811034	8.57E-51	-1.69E-46
MS.gene059272	MS.gene72400	0.817947	2.53E-52	-1.69E-46
MS.gene05954	MS.gene72400	0.80452	2.08E-49	-1.69E-46
MS.gene059570	MS.gene72400	0.815413	9.36E-52	-1.69E-46
MS.gene059584	MS.gene72400	0.818763	1.65E-52	-1.69E-46
MS.gene059601	MS.gene72400	0.848937	4.46E-60	-1.69E-46
MS.gene059602	MS.gene72400	0.805964	1.04E-49	-1.69E-46
MS.gene059625	MS.gene72400	0.843326	1.50E-58	-1.69E-46
MS.gene059842	MS.gene72400	0.813652	2.30E-51	-1.69E-46
MS.gene060011	MS.gene72400	0.826655	2.41E-54	-1.69E-46
MS.gene060137	MS.gene72400	-0.802481	5.50E-49	-1.69E-46
MS.gene060167	MS.gene72400	0.825037	5.83E-54	-1.69E-46
MS.gene060310	MS.gene72400	0.830504	2.83E-55	-1.69E-46
MS.gene060312	MS.gene72400	0.801498	8.75E-49	-1.69E-46
MS.gene060322	MS.gene72400	0.839318	1.70E-57	-1.69E-46
MS.gene060373	MS.gene72400	0.817367	3.42E-52	-1.69E-46
MS.gene060521	MS.gene72400	0.815421	9.32E-52	-1.69E-46
MS.gene06052	MS.gene72400	0.838576	2.65E-57	-1.69E-46
MS.gene060587	MS.gene72400	0.819524	1.11E-52	-1.69E-46
MS.gene060840	MS.gene72400	0.813634	2.32E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72400.t1	MTR_4g100860	93.056	144	5	3	1	144	177	315	2.78e-91	267
MS.gene72400.t1	MTR_0160s0030	41.732	127	54	4	30	144	237	355	1.06e-22	92.0
MS.gene72400.t1	MTR_4g027440	38.686	137	57	3	23	132	210	346	1.35e-20	86.3
MS.gene72400.t1	MTR_5g012630	42.553	94	32	3	36	129	188	259	2.13e-13	65.5
MS.gene72400.t1	MTR_4g109340	50.000	64	26	2	74	137	436	493	3.22e-12	63.2
MS.gene72400.t1	MTR_4g082980	50.000	62	27	1	73	134	393	450	3.93e-12	62.8
MS.gene72400.t1	MTR_7g100100	33.898	118	65	3	31	136	32	148	5.09e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72400.t1	AT3G60580	40.157	127	53	4	29	141	169	286	8.84e-23	91.3
MS.gene72400.t1	AT2G45120	39.735	151	66	5	7	143	173	312	4.82e-21	87.0
MS.gene72400.t1	AT1G02030	38.889	126	54	4	27	141	153	266	1.00e-15	72.0
MS.gene72400.t1	AT1G26610	44.000	75	37	1	60	134	386	455	1.77e-13	66.6
MS.gene72400.t1	AT5G61470	43.902	82	36	3	27	98	218	299	2.98e-12	62.8
MS.gene72400.t1	AT3G53600	34.545	110	59	4	35	135	51	156	2.26e-11	58.9

Find 24 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAAACACCTTAAAACAAAA+TGG	0.311153	8.3:+20228375	None:intergenic
TCTTTAAATCTAGCAGCAAT+AGG	0.373006	8.3:+20228261	None:intergenic
GTTTCGATCTTCAAGAGCAT+TGG	0.459910	8.3:-20228478	MS.gene72400:CDS
TTTCGATCTTCAAGAGCATT+GGG	0.469548	8.3:-20228477	MS.gene72400:CDS
TTTGGTTCTGGTCAAGCACT+TGG	0.469573	8.3:-20228357	MS.gene72400:CDS
GGTTCTGGTCAAGCACTTGG+TGG	0.484093	8.3:-20228354	MS.gene72400:CDS
TGAAGAAGAAGAGGGATCGG+TGG	0.488736	8.3:-20228571	MS.gene72400:CDS
TCATCTTCTTCAAGTGGAGC+TGG	0.500458	8.3:+20228216	None:intergenic
GCGAAGAATGGTAATGGTAA+TGG	0.505680	8.3:-20228423	MS.gene72400:CDS
AAGTTATTGAAACGAGTTCG+TGG	0.531632	8.3:-20228531	MS.gene72400:CDS
GCAGTGGAAGTTGATGAAGA+AGG	0.533864	8.3:+20228306	None:intergenic
TGTCGCGATGAAGCGAAGAA+TGG	0.548591	8.3:-20228435	MS.gene72400:CDS
CAGTGGAAGTTGATGAAGAA+GGG	0.550853	8.3:+20228307	None:intergenic
GGAACAAGATGAAGAAGAAG+AGG	0.558902	8.3:-20228580	MS.gene72400:CDS
AGATCATCATCTTCTTCAAG+TGG	0.559232	8.3:+20228210	None:intergenic
GATGAAGCGAAGAATGGTAA+TGG	0.574456	8.3:-20228429	MS.gene72400:CDS
ATTAACATTAACATTAGCAG+TGG	0.584695	8.3:+20228290	None:intergenic
AGATGAAGAAGAAGAGGGAT+CGG	0.594748	8.3:-20228574	MS.gene72400:CDS
AGTTATTGAAACGAGTTCGT+GGG	0.596995	8.3:-20228530	MS.gene72400:CDS
GAACAAGATGAAGAAGAAGA+GGG	0.633530	8.3:-20228579	MS.gene72400:CDS
AGCATTTGTGTGAAAACTGT+GGG	0.639279	8.3:-20228506	MS.gene72400:CDS
ATCGGTGGAGAAAATATCGA+AGG	0.639870	8.3:-20228556	MS.gene72400:CDS
GATGAACAACATCAACAATG+TGG	0.658258	8.3:-20228601	MS.gene72400:CDS
AAGCATTTGTGTGAAAACTG+TGG	0.714401	8.3:-20228507	MS.gene72400:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATTAACATTAACATTAGCAG+TGG	+	chr8.3:20228501-20228520	None:intergenic	25.0%
!!!	TGAATGTCCATTTTGTTTTA+AGG	-	chr8.3:20228406-20228425	MS.gene72400:CDS	25.0%
	CCAAACACCTTAAAACAAAA+TGG	+	chr8.3:20228416-20228435	None:intergenic	30.0%
	TCTTTAAATCTAGCAGCAAT+AGG	+	chr8.3:20228530-20228549	None:intergenic	30.0%
!!!	CCATTTTGTTTTAAGGTGTT+TGG	-	chr8.3:20228413-20228432	MS.gene72400:CDS	30.0%
	AAGCATTTGTGTGAAAACTG+TGG	-	chr8.3:20228281-20228300	MS.gene72400:CDS	35.0%
	AAGTTATTGAAACGAGTTCG+TGG	-	chr8.3:20228257-20228276	MS.gene72400:CDS	35.0%
	AGATCATCATCTTCTTCAAG+TGG	+	chr8.3:20228581-20228600	None:intergenic	35.0%
	AGCATTTGTGTGAAAACTGT+GGG	-	chr8.3:20228282-20228301	MS.gene72400:CDS	35.0%
	AGTTATTGAAACGAGTTCGT+GGG	-	chr8.3:20228258-20228277	MS.gene72400:CDS	35.0%
	GAACAAGATGAAGAAGAAGA+GGG	-	chr8.3:20228209-20228228	MS.gene72400:CDS	35.0%
!!	TTTCGATCTTCAAGAGCATT+GGG	-	chr8.3:20228311-20228330	MS.gene72400:CDS	35.0%
!!!	TGTTTTAAGGTGTTTGGTTC+TGG	-	chr8.3:20228419-20228438	MS.gene72400:CDS	35.0%
	AGATGAAGAAGAAGAGGGAT+CGG	-	chr8.3:20228214-20228233	MS.gene72400:CDS	40.0%
	ATCGGTGGAGAAAATATCGA+AGG	-	chr8.3:20228232-20228251	MS.gene72400:CDS	40.0%
	GATGAAGCGAAGAATGGTAA+TGG	-	chr8.3:20228359-20228378	MS.gene72400:CDS	40.0%
	GGAACAAGATGAAGAAGAAG+AGG	-	chr8.3:20228208-20228227	MS.gene72400:CDS	40.0%
!	GTTTCGATCTTCAAGAGCAT+TGG	-	chr8.3:20228310-20228329	MS.gene72400:CDS	40.0%
!!	CAGTGGAAGTTGATGAAGAA+GGG	+	chr8.3:20228484-20228503	None:intergenic	40.0%
!!	GCGAAGAATGGTAATGGTAA+TGG	-	chr8.3:20228365-20228384	MS.gene72400:CDS	40.0%
	TCATCTTCTTCAAGTGGAGC+TGG	+	chr8.3:20228575-20228594	None:intergenic	45.0%
!!	GCAGTGGAAGTTGATGAAGA+AGG	+	chr8.3:20228485-20228504	None:intergenic	45.0%
!!	TTTGGTTCTGGTCAAGCACT+TGG	-	chr8.3:20228431-20228450	MS.gene72400:CDS	45.0%
	TGAAGAAGAAGAGGGATCGG+TGG	-	chr8.3:20228217-20228236	MS.gene72400:CDS	50.0%
	TGTCGCGATGAAGCGAAGAA+TGG	-	chr8.3:20228353-20228372	MS.gene72400:CDS	50.0%
!	GGTTCTGGTCAAGCACTTGG+TGG	-	chr8.3:20228434-20228453	MS.gene72400:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	20228188	20228622	20228188	ID=MS.gene72400
chr8.3	mRNA	20228188	20228622	20228188	ID=MS.gene72400.t1;Parent=MS.gene72400
chr8.3	exon	20228188	20228622	20228188	ID=MS.gene72400.t1.exon1;Parent=MS.gene72400.t1
chr8.3	CDS	20228188	20228622	20228188	ID=cds.MS.gene72400.t1;Parent=MS.gene72400.t1

Gene Sequence

>MS.gene72400

ATGAACAACATCAACAATGTGGAACAAGATGAAGAAGAAGAGGGATCGGTGGAGAAAATATCGAAGGTGAAGTTATTGAAACGAGTTCGTGGGAAGCATTTGTGTGAAAACTGTGGGAAGACGTTTCGATCTTCAAGAGCATTGGGAAGTCATAGAAGTATTTGTTGTCGCGATGAAGCGAAGAATGGTAATGGTAATGGTAAAGATGATAAGATTTTTGAATGTCCATTTTGTTTTAAGGTGTTTGGTTCTGGTCAAGCACTTGGTGGTCACAAGAGATCTCATCTCATGATCCCTTCTTCATCAACTTCCACTGCTAATGTTAATGTTAATGTTAATCCTATTGCTGCTAGATTTAAAGAGAGTTTCATAGATCTCAATATGCCAGCTCCACTTGAAGAAGATGATGATCTTAGTGTTGTTTCTGATGCCTAA

Protein sequence

>MS.gene72400.t1

MNNINNVEQDEEEEGSVEKISKVKLLKRVRGKHLCENCGKTFRSSRALGSHRSICCRDEAKNGNGNGKDDKIFECPFCFKVFGSGQALGGHKRSHLMIPSSSTSTANVNVNVNPIAARFKESFIDLNMPAPLEEDDDLSVVSDA