AlfalfaGEDB | MS.gene48919

M. sativa cultivar XinJiangDaYe / MS.gene48919

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48919.t1	XP_028762943.1	63.8	94	28	1	12	99	255	348	2.00E-26	128.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48919.t1	A0A392P9H0	64.4	90	31	1	2	91	107	195	4.7e-25	123.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene48919	0.822337	2.50E-53	-1.69E-46
MS.gene049272	MS.gene48919	0.826258	2.99E-54	-1.69E-46
MS.gene049501	MS.gene48919	0.814256	1.69E-51	-1.69E-46
MS.gene049711	MS.gene48919	0.80295	4.40E-49	-1.69E-46
MS.gene049850	MS.gene48919	0.813755	2.18E-51	-1.69E-46
MS.gene049873	MS.gene48919	0.805791	1.13E-49	-1.69E-46
MS.gene050026	MS.gene48919	0.851076	1.12E-60	-1.69E-46
MS.gene050029	MS.gene48919	0.800757	1.24E-48	-1.69E-46
MS.gene050156	MS.gene48919	0.817728	2.84E-52	-1.69E-46
MS.gene050221	MS.gene48919	0.828224	1.01E-54	-1.69E-46
MS.gene050355	MS.gene48919	0.829793	4.22E-55	-1.69E-46
MS.gene050559	MS.gene48919	0.80258	5.24E-49	-1.69E-46
MS.gene050561	MS.gene48919	0.807709	4.43E-50	-1.69E-46
MS.gene050562	MS.gene48919	0.802365	5.81E-49	-1.69E-46
MS.gene050657	MS.gene48919	0.804186	2.44E-49	-1.69E-46
MS.gene050750	MS.gene48919	0.805953	1.04E-49	-1.69E-46
MS.gene051198	MS.gene48919	0.808313	3.29E-50	-1.69E-46
MS.gene051247	MS.gene48919	0.811933	5.46E-51	-1.69E-46
MS.gene051279	MS.gene48919	0.81546	9.14E-52	-1.69E-46
MS.gene051710	MS.gene48919	0.800442	1.44E-48	-1.69E-46
MS.gene051720	MS.gene48919	0.806051	9.92E-50	-1.69E-46
MS.gene052208	MS.gene48919	0.807568	4.74E-50	-1.69E-46
MS.gene052333	MS.gene48919	0.802425	5.64E-49	-1.69E-46
MS.gene052519	MS.gene48919	0.839939	1.17E-57	-1.69E-46
MS.gene052582	MS.gene48919	0.806883	6.63E-50	-1.69E-46
MS.gene052584	MS.gene48919	0.81288	3.39E-51	-1.69E-46
MS.gene052863	MS.gene48919	0.801241	9.88E-49	-1.69E-46
MS.gene053145	MS.gene48919	0.812139	4.93E-51	-1.69E-46
MS.gene053226	MS.gene48919	0.800203	1.61E-48	-1.69E-46
MS.gene053592	MS.gene48919	0.804296	2.31E-49	-1.69E-46
MS.gene053646	MS.gene48919	0.805784	1.13E-49	-1.69E-46
MS.gene053974	MS.gene48919	0.804258	2.35E-49	-1.69E-46
MS.gene053998	MS.gene48919	0.805008	1.64E-49	-1.69E-46
MS.gene05414	MS.gene48919	0.807055	6.09E-50	-1.69E-46
MS.gene05415	MS.gene48919	0.834155	3.53E-56	-1.69E-46
MS.gene05422	MS.gene48919	0.801409	9.13E-49	-1.69E-46
MS.gene054236	MS.gene48919	0.823003	1.75E-53	-1.69E-46
MS.gene05423	MS.gene48919	0.816403	5.63E-52	-1.69E-46
MS.gene054291	MS.gene48919	-0.80754	4.81E-50	-1.69E-46
MS.gene054651	MS.gene48919	0.834855	2.35E-56	-1.69E-46
MS.gene054775	MS.gene48919	0.809114	2.22E-50	-1.69E-46
MS.gene055466	MS.gene48919	0.811323	7.42E-51	-1.69E-46
MS.gene055589	MS.gene48919	0.801375	9.28E-49	-1.69E-46
MS.gene055818	MS.gene48919	0.823494	1.34E-53	-1.69E-46
MS.gene056050	MS.gene48919	0.806729	7.14E-50	-1.69E-46
MS.gene056162	MS.gene48919	0.802505	5.44E-49	-1.69E-46
MS.gene056174	MS.gene48919	0.828112	1.08E-54	-1.69E-46
MS.gene056595	MS.gene48919	0.836573	8.65E-57	-1.69E-46
MS.gene056854	MS.gene48919	0.823922	1.07E-53	-1.69E-46
MS.gene05686	MS.gene48919	0.800449	1.43E-48	-1.69E-46
MS.gene05690	MS.gene48919	0.801746	7.78E-49	-1.69E-46
MS.gene057026	MS.gene48919	0.812734	3.65E-51	-1.69E-46
MS.gene057444	MS.gene48919	0.823395	1.42E-53	-1.69E-46
MS.gene058037	MS.gene48919	0.842209	2.97E-58	-1.69E-46
MS.gene058515	MS.gene48919	0.808085	3.68E-50	-1.69E-46
MS.gene058625	MS.gene48919	0.852221	5.32E-61	-1.69E-46
MS.gene058979	MS.gene48919	0.811114	8.23E-51	-1.69E-46
MS.gene059015	MS.gene48919	0.824277	8.81E-54	-1.69E-46
MS.gene059272	MS.gene48919	0.963372	5.27E-122	-1.69E-46
MS.gene059276	MS.gene48919	0.840365	9.08E-58	-1.69E-46
MS.gene059584	MS.gene48919	0.83074	2.48E-55	-1.69E-46
MS.gene059601	MS.gene48919	0.803916	2.77E-49	-1.69E-46
MS.gene059602	MS.gene48919	0.802778	4.77E-49	-1.69E-46
MS.gene059625	MS.gene48919	0.835385	1.73E-56	-1.69E-46
MS.gene059842	MS.gene48919	0.80425	2.36E-49	-1.69E-46
MS.gene060011	MS.gene48919	0.800648	1.31E-48	-1.69E-46
MS.gene060167	MS.gene48919	0.806911	6.53E-50	-1.69E-46
MS.gene060312	MS.gene48919	0.800128	1.67E-48	-1.69E-46
MS.gene060322	MS.gene48919	0.828934	6.82E-55	-1.69E-46
MS.gene060521	MS.gene48919	0.818736	1.68E-52	-1.69E-46
MS.gene06052	MS.gene48919	0.808349	3.24E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48919.t1	MTR_1g036690	58.537	82	27	3	19	99	5	80	2.63e-25	90.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 15 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTTCAAAATAAGGATAAA+AGG	0.234592	4.3:+72774188	MS.gene48919:CDS
CCAACAATCACTTCAAAATA+AGG	0.244294	4.3:+72774180	MS.gene48919:CDS
TATAGGTGGGGTTCTATTCC+TGG	0.331534	4.3:-72774342	None:intergenic
TAAAGATCAACAAGATGTTT+TGG	0.333763	4.3:-72774157	None:intergenic
TGAAGATGATTATGGTAATA+TGG	0.338642	4.3:+72774240	MS.gene48919:CDS
GAAAATGATGAAGATGATTA+TGG	0.394855	4.3:+72774232	MS.gene48919:CDS
TGCTATCGTTGTGGAGGAAA+AGG	0.414829	4.3:+72774109	MS.gene48919:CDS
AGAAATCAGCAGCATCTAAG+TGG	0.464577	4.3:-72774270	None:intergenic
AATGTTGTAAAGAATGATAT+TGG	0.471241	4.3:+72774058	MS.gene48919:CDS
ACTTCAAAATAAGGATAAAA+GGG	0.487291	4.3:+72774189	MS.gene48919:CDS
GTTGTGGAGGAAAAGGTCAT+TGG	0.504176	4.3:+72774116	MS.gene48919:CDS
CATTAGAGATGAAAATATCC+AGG	0.532865	4.3:+72774324	MS.gene48919:CDS
GAGAGTATTTGCTATCGTTG+TGG	0.583351	4.3:+72774100	MS.gene48919:CDS
AGTATTTGCTATCGTTGTGG+AGG	0.599695	4.3:+72774103	MS.gene48919:CDS
GAAATCAGCAGCATCTAAGT+GGG	0.621669	4.3:-72774269	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATGTTGTAAAGAATGATAT+TGG	+	chr4.3:72774058-72774077	MS.gene48919:CDS	20.0%
!!	ACTTCAAAATAAGGATAAAA+GGG	+	chr4.3:72774189-72774208	MS.gene48919:CDS	20.0%
!	CACTTCAAAATAAGGATAAA+AGG	+	chr4.3:72774188-72774207	MS.gene48919:CDS	25.0%
!	GAAAATGATGAAGATGATTA+TGG	+	chr4.3:72774232-72774251	MS.gene48919:CDS	25.0%
!	TGAAGATGATTATGGTAATA+TGG	+	chr4.3:72774240-72774259	MS.gene48919:CDS	25.0%
!!	ATCATTTTCACATGCAAAAT+GGG	-	chr4.3:72774221-72774240	None:intergenic	25.0%
!!	TAAAGATCAACAAGATGTTT+TGG	-	chr4.3:72774160-72774179	None:intergenic	25.0%
	CATTAGAGATGAAAATATCC+AGG	+	chr4.3:72774324-72774343	MS.gene48919:CDS	30.0%
	CCAACAATCACTTCAAAATA+AGG	+	chr4.3:72774180-72774199	MS.gene48919:CDS	30.0%
!	AGATGATCAATCTTTTCATC+AGG	-	chr4.3:72774304-72774323	None:intergenic	30.0%
!	CATCATTTTCACATGCAAAA+TGG	-	chr4.3:72774222-72774241	None:intergenic	30.0%
!!!	CCTTATTTTGAAGTGATTGT+TGG	-	chr4.3:72774183-72774202	None:intergenic	30.0%
	AGAAATCAGCAGCATCTAAG+TGG	-	chr4.3:72774273-72774292	None:intergenic	40.0%
	GAAATCAGCAGCATCTAAGT+GGG	-	chr4.3:72774272-72774291	None:intergenic	40.0%
!!	AGTATTTGCTATCGTTGTGG+AGG	+	chr4.3:72774103-72774122	MS.gene48919:CDS	40.0%
!!	GAGAGTATTTGCTATCGTTG+TGG	+	chr4.3:72774100-72774119	MS.gene48919:CDS	40.0%
	GTTGTGGAGGAAAAGGTCAT+TGG	+	chr4.3:72774116-72774135	MS.gene48919:CDS	45.0%
	TGCTATCGTTGTGGAGGAAA+AGG	+	chr4.3:72774109-72774128	MS.gene48919:CDS	45.0%
!!!	AAGATGTTTTGGCGTACGAC+AGG	-	chr4.3:72774149-72774168	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	72774052	72774351	72774052	ID=MS.gene48919
chr4.3	mRNA	72774052	72774351	72774052	ID=MS.gene48919.t1;Parent=MS.gene48919
chr4.3	exon	72774052	72774351	72774052	ID=MS.gene48919.t1.exon1;Parent=MS.gene48919.t1
chr4.3	CDS	72774052	72774351	72774052	ID=cds.MS.gene48919.t1;Parent=MS.gene48919.t1

Gene Sequence	>MS.gene48919 ATGAAAAATGTTGTAAAGAATGATATTGGTCAAAGCAGCAAAAGTAATGAGAGTATTTGCTATCGTTGTGGAGGAAAAGGTCATTGGAGTCGTACCTGTCGTACGCCAAAACATCTTGTTGATCTTTACCAACAATCACTTCAAAATAAGGATAAAAGGGCTGAAACCCATTTTGCATGTGAAAATGATGAAGATGATTATGGTAATATGGAAAGTACCCACTTAGATGCTGCTGATTTCTTTGTTGATCCTGATGAAAAGATTGATCATCTCATTAGAGATGAAAATATCCAGGAATAG
Protein sequence	>MS.gene48919.t1 MKNVVKNDIGQSSKSNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLQNKDKRAETHFACENDEDDYGNMESTHLDAADFFVDPDEKIDHLIRDENIQE