AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80853


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80853.t1 MTR_6g084590 89.873 79 7 1 1 79 60 137 1.62e-44 140
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 25 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGGGAGGTGGTGATTATA+AGG 0.308115 6.2:+82975181 MS.gene80853:CDS
TTGAGAAACCGGCGGAGCTC+GGG 0.355635 6.2:+82975085 MS.gene80853:CDS
CCACCACTTTCAGCAGCTTC+CGG 0.372406 6.2:-82975231 None:intergenic
GGAAAAGATTGGTGGGGAGG+TGG 0.451985 6.2:+82975170 MS.gene80853:CDS
CTTGAGAAACCGGCGGAGCT+CGG 0.454816 6.2:+82975084 MS.gene80853:CDS
TCATCGCTGACGACCGCGGC+CGG 0.463819 6.2:+82975130 MS.gene80853:CDS
AAGGAAAAGGTGGAAGAGAA+TGG 0.465732 6.2:+82975052 MS.gene80853:CDS
GAGGGAAACTCTTGAGAAAC+CGG 0.490287 6.2:+82975074 MS.gene80853:CDS
ATTAAGAAGAAGGAAAAGAT+TGG 0.499385 6.2:+82975159 MS.gene80853:CDS
CTGACGACCCCGAGCTCCGC+CGG 0.501839 6.2:-82975093 None:intergenic
CATCGCTGACGACCGCGGCC+GGG 0.510484 6.2:+82975131 MS.gene80853:CDS
TTTGATGACAAAGGAAAAGG+TGG 0.521585 6.2:+82975042 MS.gene80853:CDS
CCGGGAAAATATTAAGAAGA+AGG 0.522927 6.2:+82975149 MS.gene80853:CDS
AGAAGAAGGAAAAGATTGGT+GGG 0.524719 6.2:+82975163 MS.gene80853:CDS
AAAAGGTGGAAGAGAATGGA+GGG 0.528096 6.2:+82975056 MS.gene80853:CDS
GCACCGGAAGCTGCTGAAAG+TGG 0.534306 6.2:+82975228 MS.gene80853:CDS
TGAGAAACCGGCGGAGCTCG+GGG 0.543054 6.2:+82975086 MS.gene80853:CDS
GAAGGAAAAGATTGGTGGGG+AGG 0.560260 6.2:+82975167 MS.gene80853:CDS
GAAAAGGTGGAAGAGAATGG+AGG 0.573388 6.2:+82975055 MS.gene80853:CDS
TGATTTGAATGATCTGGCAC+CGG 0.587113 6.2:+82975212 MS.gene80853:CDS
CCGGAAGCTGCTGAAAGTGG+TGG 0.590260 6.2:+82975231 MS.gene80853:CDS
AAGAAGAAGGAAAAGATTGG+TGG 0.596535 6.2:+82975162 MS.gene80853:CDS
GAAGAAGGAAAAGATTGGTG+GGG 0.603454 6.2:+82975164 MS.gene80853:CDS
GATATCATCGCTGACGACCG+CGG 0.641855 6.2:+82975126 MS.gene80853:CDS
GGAAACTCTTGAGAAACCGG+CGG 0.715924 6.2:+82975077 MS.gene80853:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTAAAATATTTTGATGACAA+AGG + chr6.2:82975033-82975052 MS.gene80853:CDS 20.0%
! ATTAAGAAGAAGGAAAAGAT+TGG + chr6.2:82975159-82975178 MS.gene80853:CDS 25.0%
!!! TATTTTGATGACAAAGGAAA+AGG + chr6.2:82975039-82975058 MS.gene80853:CDS 25.0%
! CCTTCTTCTTAATATTTTCC+CGG - chr6.2:82975152-82975171 None:intergenic 30.0%
!!! AGCTTTTGATTTGAATGATC+TGG + chr6.2:82975206-82975225 MS.gene80853:CDS 30.0%
AAGAAGAAGGAAAAGATTGG+TGG + chr6.2:82975162-82975181 MS.gene80853:CDS 35.0%
AGAAGAAGGAAAAGATTGGT+GGG + chr6.2:82975163-82975182 MS.gene80853:CDS 35.0%
CCGGGAAAATATTAAGAAGA+AGG + chr6.2:82975149-82975168 MS.gene80853:CDS 35.0%
! TTTGATGACAAAGGAAAAGG+TGG + chr6.2:82975042-82975061 MS.gene80853:CDS 35.0%
AAAAGGTGGAAGAGAATGGA+GGG + chr6.2:82975056-82975075 MS.gene80853:CDS 40.0%
AAGGAAAAGGTGGAAGAGAA+TGG + chr6.2:82975052-82975071 MS.gene80853:CDS 40.0%
GAAGAAGGAAAAGATTGGTG+GGG + chr6.2:82975164-82975183 MS.gene80853:CDS 40.0%
TGATTTGAATGATCTGGCAC+CGG + chr6.2:82975212-82975231 MS.gene80853:CDS 40.0%
GAAAAGGTGGAAGAGAATGG+AGG + chr6.2:82975055-82975074 MS.gene80853:CDS 45.0%
GAGGGAAACTCTTGAGAAAC+CGG + chr6.2:82975074-82975093 MS.gene80853:CDS 45.0%
! TCTTAATATTTTCCCGGCCG+CGG - chr6.2:82975146-82975165 None:intergenic 45.0%
GAAGGAAAAGATTGGTGGGG+AGG + chr6.2:82975167-82975186 MS.gene80853:CDS 50.0%
GGAAACTCTTGAGAAACCGG+CGG + chr6.2:82975077-82975096 MS.gene80853:CDS 50.0%
GTGGGGAGGTGGTGATTATA+AGG + chr6.2:82975181-82975200 MS.gene80853:CDS 50.0%
CCACCACTTTCAGCAGCTTC+CGG - chr6.2:82975234-82975253 None:intergenic 55.0%
GATATCATCGCTGACGACCG+CGG + chr6.2:82975126-82975145 MS.gene80853:CDS 55.0%
GGAAAAGATTGGTGGGGAGG+TGG + chr6.2:82975170-82975189 MS.gene80853:CDS 55.0%
CCGGAAGCTGCTGAAAGTGG+TGG + chr6.2:82975231-82975250 MS.gene80853:CDS 60.0%
CTTGAGAAACCGGCGGAGCT+CGG + chr6.2:82975084-82975103 MS.gene80853:CDS 60.0%
GCACCGGAAGCTGCTGAAAG+TGG + chr6.2:82975228-82975247 MS.gene80853:CDS 60.0%
TTGAGAAACCGGCGGAGCTC+GGG + chr6.2:82975085-82975104 MS.gene80853:CDS 60.0%
TGAGAAACCGGCGGAGCTCG+GGG + chr6.2:82975086-82975105 MS.gene80853:CDS 65.0%
TCATCGCTGACGACCGCGGC+CGG + chr6.2:82975130-82975149 MS.gene80853:CDS 70.0%
CATCGCTGACGACCGCGGCC+GGG + chr6.2:82975131-82975150 MS.gene80853:CDS 75.0%
CTGACGACCCCGAGCTCCGC+CGG - chr6.2:82975096-82975115 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 82975021 82975260 82975021 ID=MS.gene80853
chr6.2 mRNA 82975021 82975260 82975021 ID=MS.gene80853.t1;Parent=MS.gene80853
chr6.2 exon 82975021 82975260 82975021 ID=MS.gene80853.t1.exon1;Parent=MS.gene80853.t1
chr6.2 CDS 82975021 82975260 82975021 ID=cds.MS.gene80853.t1;Parent=MS.gene80853.t1
Gene Sequence

>MS.gene80853

ATGAAAGTTGTAGTAAAATATTTTGATGACAAAGGAAAAGGTGGAAGAGAATGGAGGGAAACTCTTGAGAAACCGGCGGAGCTCGGGGTCGTCAGCGACGCAGATGATATCATCGCTGACGACCGCGGCCGGGAAAATATTAAGAAGAAGGAAAAGATTGGTGGGGAGGTGGTGATTATAAGGCAAGCTTTTGATTTGAATGATCTGGCACCGGAAGCTGCTGAAAGTGGTGGCGCGTGA

Protein sequence

>MS.gene80853.t1

MKVVVKYFDDKGKGGREWRETLEKPAELGVVSDADDIIADDRGRENIKKKEKIGGEVVIIRQAFDLNDLAPEAAESGGA