AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68999


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68999.t1 MTR_6g088270 66.364 110 5 1 1 78 866 975 2.22e-38 134
MS.gene68999.t1 MTR_6g088270 48.182 110 25 1 1 78 625 734 1.81e-24 95.1
MS.gene68999.t1 MTR_6g488290 64.706 68 6 1 1 50 60 127 4.92e-19 76.6
MS.gene68999.t1 MTR_0428s0020 64.706 68 6 1 1 50 60 127 8.51e-19 76.6
MS.gene68999.t1 MTR_2g039790 56.000 75 14 2 1 56 37 111 2.46e-18 73.9
MS.gene68999.t1 MTR_6g087300 52.439 82 34 2 1 78 226 306 8.15e-15 67.4
MS.gene68999.t1 MTR_6g066260 63.265 49 18 0 26 74 48 96 1.68e-14 62.8
MS.gene68999.t1 MTR_2g039780 61.538 52 6 1 27 78 100 137 4.01e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68999.t1 AT4G29060 57.273 110 15 1 1 78 773 882 4.24e-29 108
MS.gene68999.t1 AT4G29060 50.000 110 23 1 1 78 535 644 1.68e-25 98.2
MS.gene68999.t1 AT4G29060 49.091 110 18 2 1 78 535 638 1.27e-21 87.0

Find 17 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTTCGTCACAATAGTTT+CGG 0.269335 6.2:-87029504 None:intergenic
GCAAGCATACCTCAGGAAAA+AGG 0.286057 6.2:+87029269 MS.gene68999:CDS
TCCTTCGTCACAATAGTTTC+GGG 0.358591 6.2:-87029503 None:intergenic
AGGTCTTCTCATTGCATCTC+AGG 0.406127 6.2:-87029533 None:intergenic
GCAAGTTGGTGCTTGTCCTC+AGG 0.427821 6.2:+87029454 MS.gene68999:CDS
TAATAAAGCTCTTGCTGAAA+CGG 0.448622 6.2:+87029227 MS.gene68999:CDS
AACGGAAGGCGAACTTGAAA+AGG 0.487147 6.2:+87029245 MS.gene68999:CDS
CTCACTTCTGCCAACAAAGT+CGG 0.506846 6.2:-87029399 None:intergenic
TGGCAGAAGTGAGAAATTCA+AGG 0.527151 6.2:+87029409 MS.gene68999:CDS
AAGTGAGAAATTCAAGGAGT+TGG 0.528224 6.2:+87029415 MS.gene68999:CDS
AACTGTGAAACCGACTTTGT+TGG 0.542867 6.2:+87029389 MS.gene68999:CDS
AAAAGGCGCAAGCATACCTC+AGG 0.548541 6.2:+87029262 MS.gene68999:CDS
ATATCTTCAATAGATACAAA+CGG 0.551658 6.2:-87029479 None:intergenic
GATGATCTTGCAATGCAAGT+TGG 0.583957 6.2:+87029440 MS.gene68999:CDS
TCCCGAAACTATTGTGACGA+AGG 0.603689 6.2:+87029502 MS.gene68999:CDS
AAAGCTCTTGCTGAAACGGA+AGG 0.608476 6.2:+87029231 MS.gene68999:CDS
ATAGATACAAACGGAACCTG+AGG 0.668908 6.2:-87029470 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATCTTCAATAGATACAAA+CGG - chr6.2:87029482-87029501 None:intergenic 20.0%
TAATAAAGCTCTTGCTGAAA+CGG + chr6.2:87029227-87029246 MS.gene68999:CDS 30.0%
AAGTGAGAAATTCAAGGAGT+TGG + chr6.2:87029415-87029434 MS.gene68999:CDS 35.0%
TTCCTTCGTCACAATAGTTT+CGG - chr6.2:87029507-87029526 None:intergenic 35.0%
ATAGATACAAACGGAACCTG+AGG - chr6.2:87029473-87029492 None:intergenic 40.0%
CAAGCATACCTCAGGAAAAA+GGG + chr6.2:87029270-87029289 MS.gene68999:CDS 40.0%
GATGATCTTGCAATGCAAGT+TGG + chr6.2:87029440-87029459 MS.gene68999:CDS 40.0%
GTACATACATTCACGATGCA+CGG + chr6.2:87029345-87029364 MS.gene68999:intron 40.0%
GTCTTCGGATTTCTTATCAG+CGG - chr6.2:87029304-87029323 None:intergenic 40.0%
TCCTTCGTCACAATAGTTTC+GGG - chr6.2:87029506-87029525 None:intergenic 40.0%
TGGCAGAAGTGAGAAATTCA+AGG + chr6.2:87029409-87029428 MS.gene68999:CDS 40.0%
! AACTGTGAAACCGACTTTGT+TGG + chr6.2:87029389-87029408 MS.gene68999:CDS 40.0%
!! TTCCTTCAGTTGCTAGTCTT+CGG - chr6.2:87029319-87029338 None:intergenic 40.0%
AAAGCTCTTGCTGAAACGGA+AGG + chr6.2:87029231-87029250 MS.gene68999:CDS 45.0%
AACGGAAGGCGAACTTGAAA+AGG + chr6.2:87029245-87029264 MS.gene68999:CDS 45.0%
ATCCGAAGACTAGCAACTGA+AGG + chr6.2:87029314-87029333 MS.gene68999:intron 45.0%
CTCACTTCTGCCAACAAAGT+CGG - chr6.2:87029402-87029421 None:intergenic 45.0%
GCAAGCATACCTCAGGAAAA+AGG + chr6.2:87029269-87029288 MS.gene68999:CDS 45.0%
TACATTCACGATGCACGGAT+CGG + chr6.2:87029350-87029369 MS.gene68999:intron 45.0%
TCCCGAAACTATTGTGACGA+AGG + chr6.2:87029502-87029521 MS.gene68999:CDS 45.0%
! ATGAGAGACCCTTTTTCCTG+AGG - chr6.2:87029281-87029300 None:intergenic 45.0%
! AAAAGGCGCAAGCATACCTC+AGG + chr6.2:87029262-87029281 MS.gene68999:CDS 50.0%
!! GCAAGTTGGTGCTTGTCCTC+AGG + chr6.2:87029454-87029473 MS.gene68999:CDS 55.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 87029216 87029547 87029216 ID=MS.gene68999
chr6.2 mRNA 87029216 87029547 87029216 ID=MS.gene68999.t1;Parent=MS.gene68999
chr6.2 exon 87029216 87029291 87029216 ID=MS.gene68999.t1.exon1;Parent=MS.gene68999.t1
chr6.2 CDS 87029216 87029291 87029216 ID=cds.MS.gene68999.t1;Parent=MS.gene68999.t1
chr6.2 exon 87029387 87029547 87029387 ID=MS.gene68999.t1.exon2;Parent=MS.gene68999.t1
chr6.2 CDS 87029387 87029547 87029387 ID=cds.MS.gene68999.t1;Parent=MS.gene68999.t1
Gene Sequence

>MS.gene68999

ATGATGGACTGTAATAAAGCTCTTGCTGAAACGGAAGGCGAACTTGAAAAGGCGCAAGCATACCTCAGGAAAAAGGGTCTCTCATCCGCTGATAAGAAATCCGAAGACTAGCAACTGAAGGAAGAATCAGTACATACATTCACGATGCACGGATCGGTGTTCTAATTGAAGTGAACTGTGAAACCGACTTTGTTGGCAGAAGTGAGAAATTCAAGGAGTTGGTTGATGATCTTGCAATGCAAGTTGGTGCTTGTCCTCAGGTTCCGTTTGTATCTATTGAAGATATTCCCGAAACTATTGTGACGAAGGAAAAAGAACCTGAGATGCAATGA

Protein sequence

>MS.gene68999.t1

MMDCNKALAETEGELEKAQAYLRKKVNCETDFVGRSEKFKELVDDLAMQVGACPQVPFVSIEDIPETIVTKEKEPEMQ